Publications by authors named "Xiao Yang"

2,787 Publications

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Development of Microfluidic Chip-Based Loop-Mediated Isothermal Amplification (LAMP) Method for Detection of Carbapenemase Producing Bacteria.

Microbiol Spectr 2022 Aug 18:e0032222. Epub 2022 Aug 18.

Department of Laboratory Medicine, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China.

The rapid and accurate diagnostic methods to identify carbapenemase-producing organisms (CPO) is of great importance for controlling the CPO infection. Herein, we have developed a microfluidic chip-based technique to detect CPO and assessed its clinical value in detecting CPO directly from blood cultures (BCs). The detection performance of the microfluidic chip-based LAMP amplification method was analyzed retrospectively on a collection of 192 isolates including molecularly characterized 108 CPO and 84 non-CPO and prospectively on a collection of 133 positive BCs with or without CPO suspicion, respectively. In the retrospective study, the microfluidic chip-based LAMP amplification method exhibited 87.5% accuracy (95% CI [82.0-91.5]), 97.7% sensitivity (95% CI [91.2-99.6]), 78.8% specificity (95% CI [69.5-86.0]), 79.6% positive predictive value (PPV) (95% CI [70.6-86.5]) and 97.6% negative predictive value (NPV) (95% CI [90.9-99.6]). Among the 192 isolates, 22 (11.5%) false-positives (FP) and 2 (1.0%) false negatives (FN) were observed. In the prospective study, the 133 routine isolates of positive BCs including 18 meropenem-resistant CPO and 115 non-CPO were assessed, and 4 FP were observed in non-CPO and CPO, respectively. The current method showed a total detection performance of 94.0% accuracy (95% CI [88.4-97.1]), 100.0% sensitivity (95% CI [73.2-100.0]), 93.2% specificity (95% CI [86.7-96.8]), 63.6% PPV (95% CI [40.8-82.0]) and 100.0% NPV (95% CI [95.8-100.0]). In summary, the microfluidic chip-based LAMP amplification method is reliable for the rapid screening and detection of CPO with high accuracy, sensitivity, and specificity, and could easily be implemented in clinical microbiology laboratories. Rapid and accurate identification of CPO may reduce the genetic exchanges among bacteria and prevent further dissemination of carbapenemases to non-CPO. The current method had designed microfluidic chip-based LAMP amplification method for multiplex detection of carbapenemase genes and evaluated the detection performance of the newly method. The current method can rapidly screen and detect CPO with high accuracy, sensitivity, and specificity, and could easily be implemented in clinical microbiology laboratories, as this will reduce the carbapenem resistance issues worldwide.
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http://dx.doi.org/10.1128/spectrum.00322-22DOI Listing
August 2022

Spatial profiling of chromatin accessibility in mouse and human tissues.

Nature 2022 Aug 17. Epub 2022 Aug 17.

Department of Biomedical Engineering, Yale University, New Haven, CT, USA.

Cellular function in tissue is dependent on the local environment, requiring new methods for spatial mapping of biomolecules and cells in the tissue context. The emergence of spatial transcriptomics has enabled genome-scale gene expression mapping, but the ability to capture spatial epigenetic information of tissue at the cellular level and genome scale is lacking. Here we describe a method for spatially resolved chromatin accessibility profiling of tissue sections using next-generation sequencing (spatial-ATAC-seq) by combining in situ Tn5 transposition chemistry and microfluidic deterministic barcoding. Profiling mouse embryos using spatial-ATAC-seq delineated tissue-region-specific epigenetic landscapes and identified gene regulators involved in the development of the central nervous system. Mapping the accessible genome in the mouse and human brain revealed the intricate arealization of brain regions. Applying spatial-ATAC-seq to tonsil tissue resolved the spatially distinct organization of immune cell types and states in lymphoid follicles and extrafollicular zones. This technology progresses spatial biology by enabling spatially resolved chromatin accessibility profiling to improve our understanding of cell identity, cell state and cell fate decision in relation to epigenetic underpinnings in development and disease.
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http://dx.doi.org/10.1038/s41586-022-05094-1DOI Listing
August 2022

Concentrations of heavy metals in water, sediments and aquatic organisms from a closed realgar mine.

Environ Sci Pollut Res Int 2022 Aug 17. Epub 2022 Aug 17.

Key Laboratory of Land Surface Pattern and Simulation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, China.

Mining activities can result in severe heavy metal contamination in freshwater ecosystems and lead to significant health risks. In this study, eight heavy metal concentrations in the water, sediments and aquatic organisms, including eighteen fish species, two shrimp species, one crab species and one amphibian frog species of the abandoned Shimen Realgar Mine area, were analysed. The results showed that most of the heavy metals detected in water, sediments and fish from the mine area were at relatively high levels, and historical realgar mining activities were a major source of arsenic (As) contamination in this area. We concluded that heavy metal bioaccumulation is species- and tissue-specific and is different for each element and sampling site. The concentration of heavy metals in fish was generally lower than that of the other aquatic organism species; these concentrations varied among different species with different feeding habits and habitats. The study showed that heavy metal concentrations were lower in muscle tissue than in other tissues (e.g. liver, skin, exoskeleton). A significant positive correlation between the As concentrations in sediment and fish was observed, indicating that sediment is an important factor affecting As accumulation in fish; thus, for fish protection, controlling the sources of water and sediment contamination is essential. Furthermore, the estimated daily intake (EDI) of all metals was acceptable, and the corresponding target hazard quotient (THQ) and total target hazard quotient (TTHQ) values were less than 1; hence, there was no serious health risk through fish consumption in this area.
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http://dx.doi.org/10.1007/s11356-022-22563-2DOI Listing
August 2022

YY1/ITGA3 pathway may affect trophoblastic cells migration and invasion ability.

J Reprod Immunol 2022 Jul 26;153:103666. Epub 2022 Jul 26.

Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, China. Electronic address:

Recurrent spontaneous abortion (RSA) is a disturbing pregnancy disorder experienced by ~2.5% of women attempting to conceive. The pathogenesis of RSA is still unclear. Previous findings revealed that transcription factor YIN-YANG 1(YY1) was related to the pathogenesis of RSA by influence trophoblastic cell invasion ability. Present study aimed to investigate more specific molecular mechanism of YY1 playing in trophoblastic cells. In our research, RNA-seq and Chip-seq were used to find significant changed genes between si-YY1(Knock down of YY1) HTR-8/SVneo cells(n = 3) and HTR-8/SVneo cells(n = 3). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis results suggested that Integrins related pathway maybe necessary to biological functions of trophoblastic cells. Chip-seq dataset analysis results predict YY1 can regulate ITGA3/7 expression by binding to the promoter region of ITGA3/7. Furthermore, results from chip experiment, RT-PCR, Dual-luciferase reporter gene assay showed that YY1 was able to bind to the promoter region of ITGA3 and regulate ITGA3 mRNA and protein expression. However, ITGA7 could not be significant influenced by YY1. Besides, gene silencing experiment, Western blot and Immunofluorescence assay confirmed that both YY1 and ITGA3 can accelerate phosphorylation focal adhesion kinase and affect cytoskeleton formation in HTR-8/SVneo cells. In conclusion, YY1/ITGA3 play a critical role in trophoblast invasion ability by regulating cytoskeleton formation.
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http://dx.doi.org/10.1016/j.jri.2022.103666DOI Listing
July 2022

Altered expression of Tim family molecules and an imbalanced ratio of Tim-3 to Tim-1 expression in patients with type 1 diabetes.

Front Endocrinol (Lausanne) 2022 28;13:937109. Epub 2022 Jul 28.

Key Laboratory of Diabetes Immunology, National Clinical Research Center for Metabolic Diseases, Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.

Background: T-cell immunoglobulin and mucin domain (Tim) proteins are immunomodulatory molecules that play key roles in the regulation of T-cell activation. Published studies have reported that Tim molecules are involved in the pathogenesis of certain autoimmune diseases. Type 1 diabetes (T1D) is an autoimmune disease in which T cells mediate the destruction of islet β cells. However, the expression of Tim molecules in T1D remains unclear. In this study, we measured the expression of Tim family molecules as well as T-cell subset-specific transcription factors in T1D patients, and we explored the possible involvement of Tim molecules in the pathogenesis of T1D.

Methods: Ninety T1D patients, Thirty-six type 2 diabetes (T2D) patients and forty healthy controls (HCs) were recruited for this study. Peripheral blood mononuclear cells (PBMCs) were isolated, RNA was extracted from the PBMCs and reverse transcribed into cDNA, and gene expression patterns were analysed by RT-qPCR. The expression of Tim molecules in different T-cell subsets was analysed by flow cytometry.

Results: Compared with that in HCs, the mRNA expression of Tim-1 and RORC was increased in T1D patients (=0.0355 and =0.0423, respectively), while the expression of Tim-3 was decreased (=0.0013). In addition, compared with HCs, the ratio of Tim-3 to Tim-1 expression in diabetic patients was decreased (0.0001 for T1D and 0.0387 for T2D). The ratios of T-Bet to GATA3 expression and RORC to FOXP3 expression were higher in T1D patients than in HCs (0.0042 and 0.0066, respectively). Furthermore, the T1D patients with defective islet function had more significant imbalances in the Tim-3/Tim-1 and RORC/FOXP3 ratios (<0.0001, and =0.001, respectively). Moreover, Both Tim-3 expression in CD4 T cells and the Tim-3 to Tim-1 ratio were elevated in T1D in the remission phase compared to T1D.

Conclusion: Our study revealed altered expression of Tim molecules in T1D patients. The imbalanced ratios of Tim-3/Tim-1 expression were more pronounced in T1D patients with defective islet function. However, alterations in Tim molecule expression are mitigated in T1D in the remission phase. All these findings suggest that Tim family molecules may be involved in the pathogenesis of T1D.
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http://dx.doi.org/10.3389/fendo.2022.937109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366857PMC
August 2022

Anti-melanoma effect and action mechanism of a novel chitosan-based composite hydrogel containing hydroxyapatite nanoparticles.

Regen Biomater 2022 29;9:rbac050. Epub 2022 Jul 29.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610041, China.

Hydroxyapatite nanoparticles (HANPs) have been increasingly regarded and reported due to their potential anti-tumor ability. Previously, we found that the rod-like HANPs had good application potential for cutaneous melanoma (CMM). To satisfy the actual requirements in repairing post-operative skin defects and inhibiting CMM recurrence after tumorectomy, we constructed a novel chitosan/alginate (CS/Alg) hydrogel containing the aforementioned HANPs. The cell experiments confirmed that activated mitochondrial-dependent apoptosis was tightly related to the anti-tumor ability of HANPs. Specifically, we further discovered several target proteins might be involved in abnormal activating Wnt, proteoglycans in cancer, oxidative phosphorylation and p53 signaling pathways. The animal experiments demonstrated that the HANPs-loaded CS/Alg hydrogel (CS/Alg/HANPs) had a similar effect on inhibiting tumor growth as HANPs, and CS/Alg hydrogel as well as phosphate buffered saline (PBS) group (control) not showed any effect, proving the key role of HANPs. The immunohistochemical staining demonstrated a tumor inhibition via the mitochondria-mediated apoptosis pathway, consistent with the evaluation. Moreover, CS/Alg/HANPs exhibited no additional biosafety risk to the functions of major organs. Overall, this CS/Alg/HANPs hydrogel has substantial application potential for treating CMM.
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http://dx.doi.org/10.1093/rb/rbac050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362996PMC
July 2022

UAV Localization Algorithm Based on Factor Graph Optimization in Complex Scenes.

Sensors (Basel) 2022 Aug 5;22(15). Epub 2022 Aug 5.

Dengzhou Water Conservancy Bureau, Dengzhou 474150, China.

With the increasingly widespread application of UAV intelligence, the need for autonomous navigation and positioning is becoming more and more important. To solve the problem that UAV cannot perform localization in complex scenes, a new multi-source fusion framework factor graph optimization algorithm is used for UAV localization state estimation in this paper, which is based on IMU/GNSS/VO multi-source sensors. Based on the factor graph model and the iSAM incremental inference algorithm, a multi-source fusion model of IMU/GNSS/VO is established, including the IMU pre-integration factor, IMU bias factor, GNSS factor, and VO factor. Mathematical simulations and validations on the EuRoC dataset show that, when the selected sliding window size is 30, the factor graph optimization (FGO) algorithm can not only meet the requirements of real time and accuracy at the same time, but it also achieves a plug-and-play function in the event of local sensor failures. Finally, compared with the traditional federated Kalman algorithm and the adaptive federated Kalman algorithm, the positioning accuracy of the FGO algorithm in this paper is improved by 1.5-2-fold, and can effectively improve autonomous navigation system robustness and flexibility in complex scenarios. Moreover, the multi-source fusion framework in this paper is a general algorithm framework that can satisfy other scenarios and other types of sensor combinations.
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http://dx.doi.org/10.3390/s22155862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370926PMC
August 2022

Greenhouse Gas Emissions from Beef Cattle Breeding Based on the Ecological Cycle Model.

Int J Environ Res Public Health 2022 Aug 2;19(15). Epub 2022 Aug 2.

College of Biological and Agricultural Engineering, Jilin University, 5988 Renmin Street, Changchun 130022, China.

Over the past few decades, the supply of beef has increasingly become available with the great improvement of the quality of life, especially in developing countries. However, along with the demand for meat products of high quality and the transformation of dietary structure, the impact of massive agricultural greenhouse gas emissions on the environmental load cannot be ignored. Therefore, the objective of this study is to predict the annual greenhouse gas emissions of 10 million heads of beef cattle under both the ecological cycle model (EC model) and the non-ecological cycle model (non-EC model), respectively, in order to compare the differences between these two production models in each process, and thus explore which one is more sustainable and environmentally friendly. To this end, through the life cycle assessment (LCA), this paper performs relevant calculations according to the methodology of 2019 Refinement to the 2006 IPCC Guidelines for National Greenhouse Gas Inventories (2019 IPCC Inventories). The results have shown that the total GHG emissions of the non-EC model were almost 4 times higher than those of the EC model, and feed-grain cultivation and manure management were main emission sources in both models. The non-EC model produced significantly more emissions than the EC model in each kind of GHG, especially the largest gap between these two was in CO emissions that accounted for 68.01% and 56.17% of the respective planting and breeding systems. This study demonstrates that the transformation of a beef cattle breeding model has a significant direct impact on cutting agricultural GHG emissions, and persuades other countries in the similar situation to vigorously advocate ecological cycling breeding model instead of the traditional ones so that promotes coordinated development between planting industry and beef cattle breeding industry.
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http://dx.doi.org/10.3390/ijerph19159481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367784PMC
August 2022

A Deep Learning Model for Detecting Cage-Free Hens on the Litter Floor.

Animals (Basel) 2022 Aug 5;12(15). Epub 2022 Aug 5.

Department of Computer Science, Franklin College of Arts and Sciences, University of Georgia, Athens, GA 30602, USA.

Real-time and automatic detection of chickens (e.g., laying hens and broilers) is the cornerstone of precision poultry farming based on image recognition. However, such identification becomes more challenging under cage-free conditions comparing to caged hens. In this study, we developed a deep learning model () based on , an advanced convolutional neural network (CNN), to monitor hens' behaviors in cage-free facilities. More than 1000 images were used to train the model and an additional 200 images were adopted to test it. One-way ANOVA and Tukey HSD analyses were conducted using JMP software (JMP Pro 16 for Mac, SAS Institute, Cary, North Caronia) to determine whether there are significant differences between the predicted number of hens and the actual number of hens under various situations (i.e., age, light intensity, and observational angles). The difference was considered significant at < 0.05. Our results show that the evaluation metrics (Precision, Recall, F1 and [email protected]) of the model were 0.96, 0.96, 0.96 and 0.95, respectively, in detecting hens on the litter floor. The newly developed was tested with stable performances in detecting birds under different lighting intensities, angles, and ages over 8 weeks (i.e., birds were 8-16 weeks old). For instance, the model was tested with 95% accuracy after the birds were 8 weeks old. However, younger chicks such as one-week old birds were harder to be tracked (e.g., only 25% accuracy) due to interferences of equipment such as feeders, drink lines, and perches. According to further data analysis, the model performed efficiently in real-time detection with an overall accuracy more than 95%, which is the key step for the tracking of individual birds for evaluation of production and welfare. However, there are some limitations of the current version of the model. Error detections came from highly overlapped stock, uneven light intensity, and images occluded by equipment (i.e., drinking line and feeder). Future research is needed to address those issues for a higher detection. The current study established a novel CNN deep learning model in research cage-free facilities for the detection of hens, which provides a technical basis for developing a machine vision system for tracking individual birds for evaluation of the animals' behaviors and welfare status in commercial cage-free houses.
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http://dx.doi.org/10.3390/ani12151983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367364PMC
August 2022

Dietary supplementation with perillartine ameliorates lipid metabolism disorder induced by a high-fat diet in broiler chickens.

Biochem Biophys Res Commun 2022 Aug 2;625:66-74. Epub 2022 Aug 2.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, 530004, PR China. Electronic address:

Lipid metabolism disorders affect the growth and jeopardize the health of poultry, thus, decreasing economic benefits. Perillartine, a sweetener derived from Perilla frutescens, has excellent potential in regulating lipid metabolism. In this study, we explored the effects of perillartine on lipid metabolism in broiler chickens by establishing a nonalcoholic fatty liver model induced by a high-fat diet. By using network pharmacology and molecular docking, we analyzed the potential molecular targets and pathways through which perillartine regulates lipid metabolism and alleviates fatty liver. Perillartine was found to regulate the expression of genes associated with lipogenesis, lipolysis, and lipid transport, including FASN, PPARα, CPT-1, ACCα, APOB, and APOA1 in the liver, and to decrease lipid accumulation in the liver and blood in broilers without affecting growth performance. In addition, we discovered 24 candidate targets of perillartine, including SRD5A2 and XDH, through network pharmacology analysis and successfully constructed a compound-target-pathway-disease network. Our results suggested that perillartine may be a promising, long-lasting therapeutic molecule for modulating lipid metabolism disorders in broilers.
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http://dx.doi.org/10.1016/j.bbrc.2022.07.116DOI Listing
August 2022

Latent neighborhood-based heterogeneous graph representation.

Neural Netw 2022 Jul 30;154:413-424. Epub 2022 Jul 30.

Key Laboratory of Big Data Mining and Knowledge Management, Chinese Academy of Sciences, Beijing, 100190, China. Electronic address:

Graph, as a powerful data structure, has shown superior capability on modeling complex systems. Since real-world objects and their interactions are often multi-modal and multi-typed, compared with traditional homogeneous graphs, heterogeneous graphs can represent real-world objects more effectively. Meanwhile, rich semantic information brings great challenges for learning heterogeneous graph representation (HGR). Most existing HGR methods are based on the concept of meta-path, which is constructed based on direct neighbors and define composite semantic relations in heterogeneous graph. However, when the direct neighbor information is inadequate, which always happens due to insufficient observation, the quality of meta-paths cannot be guaranteed. Therefore, we propose a novel HGR framework based on latent direct neighbors. Specifically, random walks are first utilized to discover the potential candidates from indirect neighbors. Then HodgeRank is introduced to determine the latent direct neighbors according to their importance to the target. After that, neighborhood relationships are augmented with the selected latent direct neighbors, and the adjacency tensor of the heterogeneous graph is refactored correspondingly. Finally, Graph Transformer Network is adopted to construct semantic meta-paths automatically and generate HGR. Numerical experiments on different real-world heterogeneous networks show that our new approach can produce more meta-path instances and introduce more complex and diverse semantic information, and consequently achieves more accurate predictions compared with several state-of-the-art baselines.
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http://dx.doi.org/10.1016/j.neunet.2022.07.028DOI Listing
July 2022

Economic choice and heart rate fractal scaling indicate that cognitive effort is reduced by depression and boosted by sad mood.

Biol Psychiatry Cogn Neurosci Neuroimaging 2022 Aug 7. Epub 2022 Aug 7.

University of Pittsburgh Medical Center, Department of Psychiatry.

Background: People with depression typically exhibit diminished cognitive control. Control is subjectively costly, prompting speculation that control deficits reflect reduced cognitive effort. Evidence that people with depression exert less cognitive effort is mixed, however, and motivation may depend on state affect.

Methods: We use a cognitive effort discounting task to measure propensity to expend cognitive effort and fractal structure in the temporal dynamics of inter-beat intervals to assess on-task effort exertion for 49 healthy controls, 36 people with current depression, and 67 people with remitted depression.

Results: People with depression discount more steeply, indicating that they are less willing to exert cognitive effort than are people with remitted depression and never-depressed controls. Also, steeper discounting predicts worse functioning in daily life. Surprisingly, a sad mood induction selectively boosts motivation among participants with depression, erasing differences between them and controls. During task performance, depressed participants with the lowest cognitive motivation show blunted autonomic reactivity as a function of load.

Conclusions: Discounting patterns support the hypothesis that people with current depression are less willing to exert cognitive effort, and steeper discounting predicts lower global functioning in daily life. Heart rate fractal scaling proves to be a highly sensitive index of cognitive load, and data imply that those with lower motivation for cognitive effort have a diminished physiological capacity to respond to rising cognitive demands. State affect appears to influence motivation among people with current depression given that they are more willing to exert cognitive effort following a sad mood induction.
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http://dx.doi.org/10.1016/j.bpsc.2022.07.008DOI Listing
August 2022

Development, hotspots and trend directions of groundwater salinization research in both coastal and inland areas: a bibliometric and visualization analysis from 1970 to 2021.

Environ Sci Pollut Res Int 2022 Aug 9. Epub 2022 Aug 9.

Institute of Marine Science and Technology, Shandong University, Binhai Road No. 72, Qingdao, 266237, Shandong Province, China.

As a global concern, the issue of groundwater salinization refers to the phenomenon of an increase in the overall chemical content over background levels in the groundwater. It involves a long-term process that could degrade groundwater quality and restrict its availability for drinking, irrigation and industry. For the effective protection and further research of groundwater resources, policy strongly depends on understanding the development, hotspots and trend directions of groundwater salinization research, which involves the degree, sources and processes of global groundwater salinization. However, such a comprehensive and systematic analysis has not been performed, and it is difficult to have a deeper understanding of groundwater salinization. The purpose of this paper is to analyze the knowledge structure, hot topics and trends in the field of groundwater salinization based on 6651 Web of Science (WoS) publications combined with CiteSpace for in-depth bibliometric and visual analysis. The results showed that 292 institutions in 125 countries have published articles in this field from 1970 to 2021. The USA was one of the most prolific contributors, with the largest number of publications and active institutions. Cooperation among authors has become frequent in recent years, and they tend to cooperate in groups. According to the analysis of co-occurrence keywords and co-cited articles, "water resources", "sea level rise" and "variable density flow" were identified as three hot topics. A keyword burst analysis revealed the emerging trends of concerns about global climate change and the sustainable utilization of water resources. In addition, the possible opportunities and challenges were explored that may be faced in groundwater salinization research. The outcomes of this study are significant for future research on groundwater management and pollution control.
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http://dx.doi.org/10.1007/s11356-022-22134-5DOI Listing
August 2022

Analysis of Changes in Weight, Waist Circumference, or Both, and All-Cause Mortality in Chinese Adults.

JAMA Netw Open 2022 Aug 1;5(8):e2225876. Epub 2022 Aug 1.

Department of Epidemiology and Biostatistics, Key Laboratory of Environment and Health, Ministry of Education and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Importance: Although numerous studies have separately investigated the associations of changes in weight or waist circumference with mortality risk, few studies have examined the associations of concurrent changes in these 2 anthropometric parameters with all-cause mortality.

Objective: To assess the associations of changes in body weight, waist circumference, or both, combined with all-cause mortality.

Design, Setting, And Participants: This cohort study used data from 2 longitudinal cohort studies in Dongfeng-Tongji and Kailuan, China. Participants included 58 132 adults (aged 40 years and older) with measures of weight and waist circumference at baseline and follow-up visit. Statistical analysis was performed from June 2020 to September 2021.

Exposures: Changes in weight and waist circumference between 2 visits (2008-2010 to 2013 in the Dongfeng-Tongji cohort, and 2006-2007 to 2010-2011 in the Kailuan study). Stable weight was defined as change in weight within 2.5 kg between the 2 visits and stable waist circumference was defined as changes within 3.0 cm. Changes were categorized as loss, stable, or gain for weight and waist circumference separately, and created a 9-category variable to represent the joint changes.

Main Outcomes And Measures: All-cause mortality from follow-up visit (2013 in Dongfeng-Tongji cohort and 2010-2011 in Kailuan study) until December 31, 2018. Cox proportional hazard regression models were used to estimate the associations with adjustment for potential confounders. Results were obtained in the 2 cohorts separately and pooled via fixed-effect meta-analysis.

Results: A total of 10 951 participants in the Dongfeng-Tongji cohort (median [IQR] age, 62 [56-66] years; 4203 [38.4%] men) and 47 181 participants in the Kailuan study (median [IQR] age, 51 [46-58] years; 36 663 [77.7%] men) were included in the analysis. During 426 072 person-years of follow-up, 4028 deaths (523 in the Dongfeng-Tongji cohort and 3505 in the Kailuan study) were documented. When changes in weight and waist circumference were examined separately, U-shape associations were found: both gain and loss in weight (weight loss: pooled hazard ratio [HR], 1.33; 95% CI, 1.23-1.43; weight gain: HR, 1.10; 95% CI, 1.02-1.19) or waist circumference (waist circumference loss: HR, 1.14; 95% CI, 1.05-1.24; waist circumference gain: HR, 1.11; 95% CI, 1.03-1.21) were associated with higher mortality risk compared with stable weight or waist group. When changes in weight and waist circumference were jointly assessed, compared with participants with stable weight and waist circumference (16.9% of the total population [9828 of 58 132] with 508 deaths), participants with different combinations of weight and waist circumference change all had higher mortality risks except for those with stable weight but significant loss in waist. Notably, those who lost weight but gained waist circumference (6.4% of the total population [3698 of 58 132] with 308 deaths) had the highest risk of all-cause mortality (HR, 1.69; 95% CI, 1.46-1.96; absolute rate difference per 100 000 person-years in the Dongfeng-Tongji cohort: 414; 95% CI, 116-819; and in the Kailuan study: 333; 95% CI, 195-492) among the joint subgroups.

Conclusions And Relevance: In this cohort study, weight loss with concurrent waist circumference gain was associated with a higher mortality risk in middle-aged and older Chinese adults. This study's findings suggest the importance of evaluating the changes in both body weight and waist circumference when assessing their associations with mortality.
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http://dx.doi.org/10.1001/jamanetworkopen.2022.25876DOI Listing
August 2022

Latent Autoimmune Diabetes in Adults (LADA): From Immunopathogenesis to Immunotherapy.

Front Endocrinol (Lausanne) 2022 21;13:917169. Epub 2022 Jul 21.

National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.

Latent autoimmune diabetes in adults (LADA) is a type of diabetes characterized by slow autoimmune damage of pancreatic β cells without insulin treatment in the early clinical stage. There are differences between LADA and classical type 1 diabetes (T1D) and type 2 diabetes (T2D) in genetic background, autoimmune response, rate of islet function decline, clinical metabolic characteristics, and so on. The disease progression and drug response of patients with LADA are closely related to the level of islet autoimmunity, thus exploring the pathogenesis of LADA is of great significance for its prevention and treatment. Previous studies reported that adaptive immunity and innate immunity play a critical role in the etiology of LADA. Recent studies have shown that the intestinal microbiota which impacts host immunity hugely, participates in the pathogenesis of LADA. In addition, the progression of autoimmune pancreatic β cell destruction in LADA is slower than in classical T1D, providing a wider window of opportunities for intervention. Therefore, therapies including antidiabetic drugs with immune-regulation effects and immunomodulators could contribute to promising interventions for LADA. We also shed light on potential interventions targeting the gut microbiota and gut-associated immunity, which may be envisaged to halt or delay the process of autoimmunity in LADA.
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http://dx.doi.org/10.3389/fendo.2022.917169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350734PMC
August 2022

Immune-Related Biomarkers Improve Performance of Risk Prediction Models for Survival in Patients With Hepatocellular Carcinoma.

Front Oncol 2022 22;12:925362. Epub 2022 Jul 22.

Department of Liver Surgery and Liver Transplantation, West China Hospital, Sichuan University, Chengdu, China.

Object: The prediction of hepatocellular carcinoma (HCC) prognosis faced great challenge due to tumor heterogeneity. The purpose of this study was to explore the correlation between the immune infiltrate and prognosis. Moreover, we aimed to establish a risk prediction model for survival in HCC patients based on clinicopathological and immune indicators.

Methods: In this study, 316 patients with HCC who underwent radical resection in West China Hospital from 2009 to 2014 were included. Clinicopathological data and pathological specimens were collected. H&E staining and immunohistochemical staining were performed on the pathological tissue sections. The evaluation of tumor-infiltrating lymphocyte (TIL) density was based on H&E slices, and the assessment of the expressions of CD8, CD68, Lymphocyte activation gene-3 (LAG-3), T cell immunoglobulin domain and mucin domain-3 (TIM-3), Programmed Cell Death Protein 1 (PD-1), Programmed Cell Death Ligand 1 (PD-L1), OX40, CD66b, and Tryptase. was performed on the immunohistochemical slices. A risk prediction model for survival in HCC patients was established by integrating immune-related biomarkers and clinicopathological indicators.

Results: The Barcelona Clinic Liver Cancer (BCLC) stage; the microvascular invasion status; the density of TILs; the expressing levels of CD66b, OX40, and PD-L1 in the immune cell; CD68; and CD8 were the predictors of patients' overall survival (OS). The BCLC stage; the density of TILs; and the expressions of OX40, CD68, and CD8 were associated with disease-free survival (DFS). The expressions of CD66b, CD68, OX40, and CD8 had a cumulative effect on prognosis. The area under the curve of the prediction model for OS based on clinicopathological features was improved from 0.62 to 0.74 by adding to CD8, OX40, CD68, CD66b, and TILs, whereas it was improved from 0.59 to 0.73 for the DFS prediction model.

Conclusion: Our results, if confirmed, indicated that immune-related biomarkers should be taken into account or stratified in survival analysis for HCC.
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http://dx.doi.org/10.3389/fonc.2022.925362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353009PMC
July 2022

Decreased modular segregation of the frontal-parietal network in major depressive disorder.

Front Psychiatry 2022 22;13:929812. Epub 2022 Jul 22.

Center for Cognition and Brain Disorders, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, China.

Major depressive disorder (MDD) is a common psychiatric condition associated with aberrant large-scale distributed brain networks. However, it is unclear how the network dysfunction in MDD patients is characterized by imbalance or derangement of network modular segregation. Fifty-one MDD patients and forty-three matched healthy controls (HC) were recruited in the present study. We analyzed intrinsic brain activity derived from resting-state functional magnetic resonance imaging (R-fMRI) and then examined brain network segregation by computing the participation coefficient (PC). Further intra- and inter-modular connections analysis were preformed to explain atypical PC. Besides, we explored the potential relationship between the above graph theory measures and symptom severity in MDD. Lower modular segregation of the frontal-parietal network (FPN) was found in MDD compared with the HC group. The MDD group exhibited increased inter-module connections between the FPN and cingulo-opercular network (CON), between the FPN and cerebellum (Cere), between the CON and Cere. At the nodal level, the PC of the anterior prefrontal cortex, anterior cingulate cortex, inferior parietal lobule (IPL), and intraparietal sulcus showed larger in MDD. Additionally, the inter-module connections between the FPN and CON and the PC values of the IPL were negatively correlated with depression symptom in the MDD group. These findings might give evidence about abnormal FPN in MDD from the perspective of modular segregation in brain networks.
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http://dx.doi.org/10.3389/fpsyt.2022.929812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353222PMC
July 2022

Individual and joint associations of co-exposure to multiple plasma metals with telomere length among middle-aged and older Chinese in the Dongfeng-Tongji cohort.

Environ Res 2022 Aug 4;214(Pt 3):114031. Epub 2022 Aug 4.

Department of Occupational and Environmental Health, Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, And State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China. Electronic address:

Studies on associations of metals with leucocyte telomere length (LTL) were mainly limited to several most common toxic metals and single-metal effect, but the impact of other common metals and especially the overall joint associations and interactions of metal mixture with LTL are largely unknown. We included 15 plasma metals and LTL among 4906 participants from Dongfeng-Tongji cohort. Multivariable linear regression was used to estimate associations of individual metals with LTL. We also applied Bayesian kernel machine regression (BKMR) and quantile g-computation regression (Q-g) to evaluate the overall association and interactions, and identified the major contributors as well as the potential modifications by major characteristics. Multivariable linear regression found vanadium, copper, arsenic, aluminum and nickel were negatively associated with LTL, and a 2-fold change was related to 1.9%-5.1% shorter LTL; while manganese and zinc showed 3.7% and 4.0% longer LTL (all P < 0.05) in multiple-metal models. BKMR confirmed above metals and revealed a linearly inverse joint association between 15 metals and LTL. Q-g regression further indicated each quantile increase in mixture was associated with 5.2% shorter LTL (95% CI: -8.1%, -2.3%). Furthermore, manganese counteracted against aluminum and vanadium respectively (P<0.05). In addition, associations of vanadium, aluminum and metal mixture with LTL were more prominent in overweight participants. Our results are among the first to provide a new comprehensive view of metal mixture exposure on LTL attrition in the general population, including identifying the major components, metals interactions and the overall effects.
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http://dx.doi.org/10.1016/j.envres.2022.114031DOI Listing
August 2022

In vivo phytotoxic effect of yttrium-oxide nanoparticles on the growth, uptake and translocation of tomato seedlings (Lycopersicon esculentum).

Ecotoxicol Environ Saf 2022 Aug 2;242:113939. Epub 2022 Aug 2.

University of Wuppertal, School of Architecture and Civil Engineering, Institute of Foundation Engineering, Water, and Waste-Management, Laboratory of Soil, and Groundwater-Management, Pauluskirchstraße 7, 42285 Wuppertal, Germany; International Research Centre of Nanotechnology for Himalayan Sustainability (IRCNHS), Shoolini University, Solan 173212, Himachal Pradesh, India. Electronic address:

The potential toxicity and ecological risks of rare-earth nanoparticles in the environment have become a concern due to their widespread application and inevitable releases. The integration of hydroponics experiments, partial least squares structural equation modeling (PLS-SEM), and Transmission Electron Microscopy (TEM) were utilized to investigate the physiological toxicity, uptake and translocation of yttrium oxide nanoparticles (YO NPs) under different hydroponic treatments (1, 5, 10, 20, 50 and 100 mg·L of YO NPs, 19.2 mg·L Y(NO) and control) in tomato (Lycopersicon esculentum) seedlings. The results indicated that YO NPs had a phytotoxic effect on tomato seedlings' germination, morphology, physiology, and oxidative stress. The YO NPs and soluble Y reduced the root elongation, bud elongation, root activity, chlorophyll, soluble protein content and superoxide dismutase and accelerated the proline and malondialdehyde in the plant with increasing concentrations. The phytotoxic effects of YO NPs on tomato seedlings had a higher phytotoxic effect than soluble Y under the all treatments. The inhibition rates of different levels of YO NPs in shoot and root biomass ranged from 0.2% to 6.3% and 1.0-11.3%, respectively. The bioaccumulation and translocation factors were less than 1, which suggested that YO NPs significantly suppressed shoot and root biomass of tomato seedlings and easily bioaccumulated in the root. The observations were consistent with the process of concentration-dependent uptake and translocation factor and confirmed by TEM. YO NPs penetrate the epidermis, enter the cell wall, and exist in the intercellular space and cytoplasm of mesophyll cells of tomato seedlings by endocytic pathway. Moreover, PLS-SEM revealed that the concentration of NPs significantly negatively affects the morphology and physiology, leading to the change in biomass of plants. This study demonstrated the possible pathway of YO NPs in uptake, phytotoxicity and translocation of YO NPs in tomato seedlings.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113939DOI Listing
August 2022

[Family analysis of a child with Short-rib polydactyly syndrome type III due to variant of DYNC2H1 gene].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2022 Aug;39(8):881-883

Reproductive Center and Prenatal Diagnosis Center, the First Hospital of Jilin University, Changchun, Jilin 130021, China.

Objective: To report on the clinical characteristics of a family of short-rib polydactyly syndrome type III and its pathogenic variants.

Methods: Muscle samples from the the third fetus was collected after the induction of labor, and peripheral blood samples of its parents and grandparents were also collected. Whole exome sequencing (WES) was carried out for the pedigree. Candidate variants were verified by Sanger sequencing of the family.

Results: The proband was found to harbor a c.9819+1G>A variant and a c.4625C>A variant of the DYNC2H1 gene, which were respectively inherited from its mother and father. Sanger sequencing verified that the family has fit the autosomal recessive inheritance.

Conclusion: The c.9819+1G>A and c.4625C>A variants of the DYNC2H1 gene probably underlay the short-rib polydactyly syndrome type 3 in the proband.
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http://dx.doi.org/10.3760/cma.j.cn511374-20210628-00549DOI Listing
August 2022

Expandable, biodegradable, bioactive quaternized gelatin sponges for rapidly controlling incompressible hemorrhage and promoting wound healing.

Biomater Adv 2022 May 29;136:212776. Epub 2022 Mar 29.

Department of Intensive Care, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang 325011, China. Electronic address:

Designing expandable sponges with biodegradability and effective antibacterial properties are the urgent challenge for incompressible hemorrhage and wound healing. In the present investigation, based on quaternized gelatin (QG) and oxidized dextran (OD), a series of expandable sponges (ODQG) with high-water absorption capacity and robust mechanical properties were prepared. ODQG had good biodegradability in vitro and in vivo, and had inherent antibacterial activity (90% for E. coli and 99.74% for S. aureus). Due to the synergy effect of electrostatic interaction and blood concentration, ODQG could effectively attract and activate red blood cells/platelets and accelerate the coagulation process. Therefore, ODQG showed better hemostatic performance than Kuaikang® gelatin sponges and gauzes in incompressible hemorrhage model. Furthermore, ODQG could regulate inflammatory factor (TNF-α) and cytokines (TGF-β, VEGF), and greatly promote wound healing process. The biodegradable sponges with excellent antibacterial properties might have potential application prospect for incompressible hemostasis and wound healing in the future.
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http://dx.doi.org/10.1016/j.bioadv.2022.212776DOI Listing
May 2022

Controlling sustained statins release in multi-layered composite scaffolds for healing of osteoporotic bone defects.

Biomater Adv 2022 Jun 5;137:212838. Epub 2022 May 5.

Department of Orthopaedic Surgery, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Science, Affiliated Hospital of University of Electronic Science and Technology, Chengdu 610072, China. Electronic address:

The risk of fragility fracture sharply increases due to the decreased bone mineral density and toughness in patients with osteoporosis (OP). The local use of bone tissue scaffolds with both mechanical stability and drug-delivery functionality is one of the key strategies for the efficient curing of OP. In this work, we reported a layer-by-layer constructing strategy to fabricate 3-D composite bone tissue scaffolds (eSTPS) by assembling β-tri‑calcium phosphate (β-TCP)/polycaprolactone (PCL) microchips and lovastatin-loaded nanofiber membranes (eLOV/PCL). The eSTPS scaffolds show a strong and suited compressive strength as well as long-term delivery of lovastatin. The in vitro tests indicate well biocompatibility and alkaline phosphatase activity of the scaffolds. The eSTPS scaffolds were implanted into the femur of OP modeled rabbits. After 12 weeks curing, the bone parameters are significantly improved, meanwhile ingrowth of new bone and vascular-like tissue were observed. These results suggest the eSTPS scaffolds to be a promising candidate for the local treatment of OP.
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http://dx.doi.org/10.1016/j.bioadv.2022.212838DOI Listing
June 2022

[A comparison of clinical characteristics between acute fatty liver of pregnancy and hemolysis, elevated liver enzymes and low platelets syndrome].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2022 Jun;34(6):624-629

Department of Critical Care Medicine, Yan'an Hospital of Kunming City, Kunming 650051, Yunnan, China. Corresponding author: Xu Wangbin, Email:

Objective: To compare and analyze the clinical characteristics between acute fatty liver of pregnancy (AFLP) and the hemolysis, elevated liver enzymes and low platelets (HELLP) syndrome.

Methods: This is a retrospective cohort study. The clinical data of 13 cases with AFLP and 34 cases with HELLP syndrome were collected from three tertiary referral centers in Yunnan (the First Affiliated Hospital of Kunming Medical University, the Second Affiliated Hospital of Kunming Medical University, and Yan'an Hospital of Kunming City) from January 2016 to December 2021. The patients were diagnosed to AFLP and HELLP syndrome according to the Swansea criteria and the Tennessee classification system. The general characteristics, clinical features, laboratory results within 24 hours after admission, complications, maternal and neonatal outcomes were compared to analysis the differences between the two groups.

Results: (1) Maternal characteristics: compared with HELLP syndrome group, AFLP group had lower body mass index (BMI) and blood pressure at admission (both P < 0.01). (2) Clinical features: the most common symptoms in AFLP patients were skin jaundice, abdominal pain, nausea and vomiting, edema. The main manifestations of patients with HELLP syndrome were albuminuria, hypertension, edema, headache. Some patients had multiple symptoms concurrently. (3) Laboratory results: compared with HELLP syndrome group, the levels of platelet count (PLT), total bilirubin (TBil), direct bilirubin (DBil), γ-glutamyl transferase (γ-GGT), alkaline phosphatase (ALP), total bile acid (TBA), serum creatinine (SCr) and international standardized ratio (INR) in AFLP group were significantly increased within 24 hours after admission [PLT (×10/L): 107.69±51.13 vs.76.71±43.25,TBil (μmol/L): 121.60 (83.20, 170.00) vs.15.25 (7.22, 29.05), DBil (μmol/L): 86.50 (58.60, 104.00) vs. 4.30 (2.22,10.10), γ-GGT (U/L): 87.00 (37.00, 127.00) vs. 41.00 (19.00,64.42), ALP (U/L): 199.10 (109.00, 349.20) vs. 125.50 (90.50, 155.25), TBA (μmol/L): 51.50 (16.20, 117.40) vs. 4.15 (2.02, 6.95), SCr (μmol/L): 155.80 (129.00, 237.00) vs. 79.00 (65.43, 113.70), INR: 1.28 (1.17, 1.63) vs. 0.94 (0.88, 1.08), all P < 0.05], prothrombin time (PT) was significantly prolonged [seconds: 16.10 (14.50, 19.20) vs. 12.40 (11.43, 13.40), P < 0.05]. The level of blood glucose (GLU), fibrinogen (FIB) and the activity of antithrombin III (AT III) decreased significantly [GLU (mmol/L): 5.18±1.33 vs. 6.33±1.19, FIB (g/L): 1.96±1.46 vs. 3.81±1.58, AT III (%): 40.61±25.84 vs. 66.39±24.11, all P < 0.05]; (4) Complications: compared with HELLP syndrome group, the incidence of patients with hypoglycemia [30.77% (4/13) vs. 0% (0/34)], acute liver failure [53.85% (7/13) vs. 5.88% (2/34)], acute renal insufficiency [69.23% (9/13) vs. 8.82% (3/34)], coagulopathy [76.92% (10/13) vs. 38.24% (13/34)], disseminated intravascular coagulation (DIC) [53.85% (7/13) vs. 5.88% (2/34)], and multiple organ dysfunction syndrome (MODS) [53.85% (7/13) vs. 5.88% (2/34)] were significantly higher in AFLP group (all P <0.05). (5) Maternal and neonatal outcome: all patients delivered after admission. The total length of hospital and intensive care unit stay were significantly longer in the AFLP group than in the HELLP syndrome group [days: 17.00 (11.00, 25.00) vs. 9.00 (7.00, 12.00), 12.00 (4.00, 22.00) vs. 3.91 (0, 7.00), both P < 0.01]. Two AFLP patients died, including one due to intracranial venous thrombosis and one due to multiple organ failure and cardiopulmonary arrest. There were no deaths in the HELLP syndrome group.

Conclusions: There are significant differences in maternal characteristics, laboratory results and complications between AFLP and HELLP syndrome. TBil, γ-GGT, SCr, FIB, INR and AT III activity may help to distinguish the two diseases.
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http://dx.doi.org/10.3760/cma.j.cn121430-20220411-00361DOI Listing
June 2022

Characterization of the complete mitochondrial genome of and phylogenetic studies of Sisoridae.

Mitochondrial DNA B Resour 2022 28;7(7):1408-1409. Epub 2022 Jul 28.

Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing, China.

The entire mitochondrial genome (mitogenome) of was sequenced; it spanned 16,536 bp in length and contained 13 protein-coding genes (PCGs), 2 ribosomal RNAs, and 22 transfer RNA genes. A total of 37 genes formed a typical vertebrate mitochondrial gene arrangement. The phylogenetic tree of Sisoridae based on 13 PCGs was constructed and supported that was closely related to , , , and . The mitogenome of described in this study provided molecular evidence for its current taxonomic status and laid a groundwork for further study concerning phylogenetics within Sisoridae.
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http://dx.doi.org/10.1080/23802359.2022.2093666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341339PMC
July 2022

The pattern glare and visual memory are disrupted in patients with major depressive disorder.

BMC Psychiatry 2022 08 2;22(1):518. Epub 2022 Aug 2.

Psychiatric Laboratory and Mental Health Center, West China Hospital of Sichuan University, Chengdu, China.

Background: Visual memory impairment is one of the most commonly complained symptoms in patients with major depressive disorder (MDD). Pattern glare is also a distorted visual phenomenon that puzzles patients with MDD. Nevertheless, how these two phenomena interact in MDD remains unknown. This study investigated the association between pattern glare and visual memory in MDD patients.

Methods: Sixty-two patients with MDD and forty-nine age-, sex- and education level-matched healthy controls (HCs) were included in this study. The Pattern Recognition Memory (PRM) test and the Brief Visual Memory Test-Revised (BVMT-R) were applied to measure visual memory. The pattern glare test including three patterns with different spatial frequencies (SFs) was used to explore pattern glare levels.

Results: Patients with MDD scored lower on the PRM-PCi, BVMT-R1, BVMT-R2, BVMT-R3, and BVMT-Rt and higher on the PRM-MCLd than HCs (all p < 0.05). Pattern glare scores for MDD patients were higher with mid-SF (p < 0.001), high-SF (p = 0.006) and mid-high SF differences (p = 0.01) than for HCs. A positive correlation between mid-SF and PRM-MCLd scores in all participants was observed (p = 0.01, r = 0.246). A negative correlation between mid-high difference scores and BVMT-R2 scores (p = 0.032, r = -0.317) was observed in HCs, but no significant correlation was observed in MDD patients.

Conclusions: The present study showed that visual memory and pattern glare are disrupted in MDD. Visual memory may be associated with pattern glare and needs to be studied in future work.
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http://dx.doi.org/10.1186/s12888-022-04167-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344705PMC
August 2022

Organic covalent modification to improve thermoelectric properties of TaS.

Nat Commun 2022 Jul 29;13(1):4401. Epub 2022 Jul 29.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Organic semiconductors are attracting considerable attention as a new thermoelectric material because of their molecular diversity, non-toxicity and easy processing. The side chains which are introduced into two-dimensional (2D) transition metal dichalcogenides (TMDs) by covalent modification lead to a significant decrease in their thermal conductivity. Here, we describe a simple approach to preparing the side chains covalent modification TaS (SCCM-TaS) organic/inorganic hybrid structures, which is a homogeneous and non-destructive technique that does not depend on defects and boundaries. Electrical conductivity of 3,401 S cm and a power factor of 0.34 mW m K are obtained for a hybrid material of SCCM-TaS, with an in-plane thermal conductivity of 4.0 W m K, which is 7 times smaller than the thermal conductivity of the pristine TaS crystal. The power factor and low thermal conductivity contribute to a thermoelectric figure of merit (ZT) of ~0.04 at 443 K.
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http://dx.doi.org/10.1038/s41467-022-32058-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338255PMC
July 2022

Indocyanine green for targeted imaging of the gall bladder and fluorescence navigation.

J Biophotonics 2022 Jul 29:e202200142. Epub 2022 Jul 29.

Scientific Research Center and Precision Medical Institute, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.

Researchers nowadays have devoted extra attention to the different biomedical applications of indocyanine green (ICG), a US Food and Drug Administration-approved fluorescent compound in the fields such as drug delivery, medical imaging and disease diagnosis. In addition, hepatic function evaluation could be conducted by using ICG before surgical procedures and angiographic assessment of blood. Therefore, ICG will be expected to be excellent imaging and targeting agent in various preclinical and clinical model systems. However, whether ICG possesses the potential for the gall bladder's intraoperative imaging guidance needs to be further explored in vivo animal experiments. Herein, near-infrared fluorophores ICG can display the specific uptake by the gall bladder cells and tissues. The dynamic process of biodistribution and the clearance of ICG in vivo in mice are clearly shown in real-time live-body imaging. Furthermore, ICG was rapidly excreted into the bile and lately biodistributed to the stomach after treatment in mice. Meanwhile, the signal-to-background ratio of the gall bladder demonstrated a tremendously higher level compared to other organs (stomach, heart, liver, lung, pancreas, spleen, intestine and duodenum). In conclusion, fluorescence navigation using ICG fluorescence imaging will provide good visualization and detection of the target lesions (gall bladder) in clinics such as diagnostic medical imaging and intraoperative navigation.
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http://dx.doi.org/10.1002/jbio.202200142DOI Listing
July 2022

Two Children With Steroid-Resistant Significant Proteinuria Due to Nonsense Mutations of the Gene: A Case Report and Literature Review.

Front Pediatr 2022 12;10:918373. Epub 2022 Jul 12.

Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Background: gene mutations have been reported as the genetic basis of autosomal dominant (AD) neuro-renal syndrome in children, which presents with epileptic encephalopathy, focal segmental glomerulosclerosis (FSGS), developmental delay, and mental retardation. In this study, we report the cases of two children with significant proteinuria due to de novo nonsense mutations of the gene.

Case Presentation: Case 1 was a 7-year-old girl who presented with proteinuria and developmental delay, and her renal biopsy showed FSGS. She developed end-stage renal disease (ESRD) 3 years after onset. Case 2 was another 7-year-old girl who developed proteinuria only at age 3, and renal biopsy showed glomerular segmental mesangial proliferative lesions. The two girls underwent genetic testing but we did not find a positive result in the whole exon. However, cluster analysis revealed two new nonsense mutations of the gene (c.1461C>A, p.Tyr 487 and c.1453C>T, p.Gln485).

Conclusions: We reported the clinical manifestation of this neuro-renal syndrome for the first time in China. It is necessary to perform genetic testing in children with steroid-resistant significant proteinuria to identify its etiology and avoid the side effects of immunosuppressants.
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http://dx.doi.org/10.3389/fped.2022.918373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315245PMC
July 2022

Abnormal Dynamic Functional Connectivity of the Left Rostral Hippocampus in Predicting Antidepressant Efficacy in Major Depressive Disorder.

Psychiatry Investig 2022 Jul 21;19(7):562-569. Epub 2022 Jul 21.

Center for Cognition and Brain Disorders, The Affiliated Hospital and Institutes of Psychological Sciences, Hangzhou Normal University, Hangzhou, China.

Objective: Some pharmacological treatments are ineffective in parts of patients with major depressive disorder (MDD), hence this needs prediction of effective treatment responses. The study aims to examine the relationship between dynamic functional connectivity (dFC) of the hippocampal subregion and antidepressant improvement of MDD patients and to estimate the capability of dFC to predict antidepressant efficacy.

Methods: The data were from 70 MDD patients and 43 healthy controls (HC); the dFC of hippocampal subregions was estimated by sliding-window approach based on resting-state functional magnetic resonance imaging (R-fMRI). After 3 months treatment, 36 patients underwent second R-fMRI scan and were then divided into the response group and non-response group according to clinical responses.

Results: The result manifested that MDD patients exhibited lower mean dFC of the left rostral hippocampus (rHipp.l) compared with HC. After 3 months therapy, the response group showed lower dFC of rHipp.l compared with the non-response group. The dFC of rHipp.l was also negatively correlated with the reduction rate of Hamilton Depression Rating Scale.

Conclusion: These findings highlighted the importance of rHipp in MDD from the dFC perspective. Detection and estimation of these changes might demonstrate helpful for comprehending the pathophysiological mechanism and for assessment of treatment reaction of MDD.
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http://dx.doi.org/10.30773/pi.2021.0386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334807PMC
July 2022

Cross-platform analysis reveals cellular and molecular landscape of glioblastoma invasion.

Neuro Oncol 2022 Jul 28. Epub 2022 Jul 28.

Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA.

Background: Improved treatment of glioblastoma (GBM) needs to address tumor invasion, a hallmark of the disease that remains poorly understood. In this study, we profiled GBM invasion through integrative analysis of histological and single-cell RNA sequencing (scRNA-seq) data from ten patients.

Methods: Human histology samples, patient-derived xenograft mouse histology samples, and scRNA-seq data were collected from ten GBM patients. Tumor invasion was characterized and quantified at the phenotypic level using H&E and Ki-67 histology stains. Crystallin alpha B (CRYAB) and CD44 were identified as regulators of tumor invasion from scRNA-seq transcriptomic data and validated in vitro, in vivo, and in a mouse GBM resection model.

Results: At the cellular level, we found that invasive GBM are less dense and proliferative than their non-invasive counterparts. At the molecular level, we identified unique transcriptomic features that significantly contribute to GBM invasion. Specifically, we found that CRYAB significantly contributes to post-operative recurrence and is highly co-expressed with CD44 in invasive GBM samples.

Conclusions: Collectively, our analysis identifies differentially expressed features between invasive and nodular GBM, and describes a novel relationship between CRYAB and CD44 that contributes to tumor invasiveness, establishing a cellular and molecular landscape of GBM invasion.
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http://dx.doi.org/10.1093/neuonc/noac186DOI Listing
July 2022
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