Publications by authors named "Xiao Fu"

264 Publications

Prognostic Nutritional Index identifies risk of early progression and survival outcomes in Advanced Non-small Cell Lung Cancer patients treated with PD-1 inhibitors.

J Cancer 2021 15;12(10):2960-2967. Epub 2021 Mar 15.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, No.277 Yanta West Road, Xi'an, Shaanxi 710061, People's Republic of China.

The prognostic nutritional index (PNI) is related to the prognosis of multiple malignancies. This study investigated whether the PNI has prognostic value in advanced non-small cell lung cancer (NSCLC) patients treated with programmed death 1 (PD-1) inhibitors. We retrospectively analyzed advanced NSCLC patients treated with PD-1 inhibitors from July 2018 to December 2019. Pretreatment PNI was calculated by peripheral lymphocyte count and serum albumin level, and the cut-off value was determined. Subsequently, we investigated the relationship between PNI and early progression, and evaluated its prognostic role on survival outcomes. Ultimately, based on the results of survival analysis, a nomogram was established. A total of 123 patients were included. Of these, 24 (19.5%) patients had experienced early progression. Multivariate logistic analysis indicated that low PNI (odds ratio, 3.709, 95% confidence interval [CI], 1.354-10.161; = 0.011) was closely correlated with early progression. Moreover, multivariate Cox regression analysis confirmed that low PNI was an independent risk factor for progression-free survival (hazard ratio [HR], 2.698, 95% CI, 1.752-4.153; < 0.001) and overall survival (HR, 7.222, 95% CI, 4.081-12.781; < 0.001), respectively. The prediction accuracy of nomogram based on PNI is moderate. PNI was an independent predictor of early progression and survival outcomes in advanced NSCLC patients treated with PD-1 inhibitors.
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http://dx.doi.org/10.7150/jca.55936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040881PMC
March 2021

Thin endometrium is associated with the risk of hypertensive disorders of pregnancy in fresh IVF/ICSI embryo transfer cycles: a retrospective cohort study of 9,266 singleton births.

Reprod Biol Endocrinol 2021 Apr 9;19(1):55. Epub 2021 Apr 9.

Center for Reproductive Medicine, Shandong University, No.157 Jingliu Road, Jinan, 250021, China.

Background: Thin endometrial thickness (EMT) has been suggested to be associated with reduced incidence of pregnancy rate after in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment, but the effect of thin endometrium on obstetric outcome is less investigated. This study aims to determine whether EMT affects the incidence of obstetric complications in fresh IVF/ICSI-embryo transfer (ET) cycles.

Methods: We conducted a retrospective cohort study collecting a total of 9266 women who had singleton livebirths after fresh IVF/ICSI-ET treatment cycles at the Center for Reproductive Medicine Affiliated to Shandong University between January 2014 and December 2018. The women were divided into three groups according to the EMT: 544 women with an EMT ≤8 mm, 6234 with an EMT > 8-12 mm, and 2488 with an EMT > 12 mm. The primary outcomes were the incidence of obstetric complications including hypertensive disorders of pregnancy (HDP), gestational diabetes mellitus (GDM), placental abruption, placenta previa, postpartum hemorrhage (PPH) and cesarean section. Multivariable logistic regression analysis was performed to calculate the odds ratios (ORs) and 95% confidence intervals (CIs) for associations between the EMT measured on the day of human chorionic gonadotropin (HCG) trigger and the risk of the outcomes of interest.

Results: The HDP incidence rate of pregnant women was highest in EMT ≤ 8 mm group and significantly higher than those in EMT from > 8-12 mm and EMT > 12 mm group, respectively (6.8% versus 3.6 and 3.5%, respectively; P = 0.001). After adjustment for confounding variables by multivariate logistic regression analysis, a thin EMT was still statistically significant associated with an increased risk of HDP. Compared with women with an EMT > 8-12 mm, women with an EMT ≤8 mm had an increased risk of HDP (aOR = 1.853, 95% CI 1.281-2.679, P = 0.001).

Conclusion: A thin endometrium (≤8 mm) was found to be associated with an increased risk of HDP after adjustment for confounding variables, indicating that the thin endometrium itself is a risk factor for HDP. Obstetricians should remain aware of the possibility of HDP when women with a thin EMT achieve pregnancy through fresh IVF/ICSI-ET treatment cycles.
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http://dx.doi.org/10.1186/s12958-021-00738-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034143PMC
April 2021

The complete chloroplast genome of leguminous forage .

Mitochondrial DNA B Resour 2021 Mar 15;6(3):898-899. Epub 2021 Mar 15.

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou China.

is mainly distributed in Europe and has been widely cultivated in North and Northwest of China. The complete chloroplast genome was sequenced using the Illumina Hiseq X-Ten platform. The genome lacks an inverted repeat (IR) region, containing 76 protein-coding genes, 30 tRNAs genes and 4 rRNAs. The overall GC content is 34.6%. A phylogenetic tree based on the whole chloroplast genomes of 14 species indicated that belonged to the tribe Hydysareae in IRLC group of the subfamily Papilionoideae (Leguminosae), and it was sister to the genus .
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http://dx.doi.org/10.1080/23802359.2021.1886017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971300PMC
March 2021

Zinc finger protein 91 accelerates tumour progression by activating β-catenin signalling in pancreatic cancer.

Cell Prolif 2021 May 23;54(5):e13031. Epub 2021 Mar 23.

Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Objectives: ZFP91, an E3 ligase, has been reported to possess cancer-promoting functions. This study aimed to elucidate the exact role of ZFP91 in tumour progression of pancreatic cancer and underlying mechanisms.

Materials And Methods: We analysed the correlation between ZFP91 expression and pancreatic cancer through TCGA and GEO data sets. Growth curve, colony formation, wound healing and transwell invasion assays were conducted to evaluate proliferation, migration and invasion of lentivirus transfected pancreatic cancer cells. GSEA and Western blot analysis were performed to validate the regulatory effect of ZFP91 on β-catenin. Drug response curve and orthotopic implantation model reflected the sensitivity of chemotherapies.

Results: ZFP91 overexpression is prevalent in pancreatic cancer and negatively correlated with overall survival. ZFP91 knock-down attenuated proliferation, migration and invasion of pancreatic cancer cells. β-catenin was a downstream gene of ZFP91, and its agonist could reverse the phenotype. ZFP91 promoted EMT and chemoresistance in pancreatic cancer.

Conclusions: We demonstrated that ZFP91 promoted pancreatic cancer proliferation, migration and invasion through activating β-catenin signalling. EMT and chemoresistance were also regulated by ZFP91. ZFP91 might be a potential therapeutic target for pancreatic cancer.
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http://dx.doi.org/10.1111/cpr.13031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088462PMC
May 2021

Poly(ethylene glycol)-Mediated Assembly of Vaccine Particles to Improve Stability and Immunogenicity.

ACS Appl Mater Interfaces 2021 Mar 22;13(12):13978-13989. Epub 2021 Mar 22.

School of Physics and Electronic Science, East China Normal University, Shanghai 200241, China.

We report the one-step assembly of vaccine particles by encapsulating ovalbumin (OVA) and cytosine-phosphate-guanine oligodeoxynucleotides (CpG) into poly(ethylene glycol) (PEG)-mediated zeolitic imidazolate framework-8 nanoparticles (OVA-CpG@ZIF-8 NPs), where PEG improves the stability and dispersity of ZIF-8 NPs and the NPs protect the encapsulated OVA and CpG to circumvent the cold chain issue. Compared with free OVA and OVA-encapsulated ZIF-8 (OVA@ZIF-8) NPs, OVA-CpG@ZIF-8 NPs can enhance antigen uptake, cross-presentation, dendritic cell (DC) maturation, production of specific antibody and cytokines, and CD4 T and CD8 T cell activation. More importantly, the vaccine particles retain their bioactivity against enzymatic degradation, elevated temperatures, and long-term storage at ambient temperature. The study highlights the importance of PEG-mediated ZIF-8 NPs as a vaccine delivery system for the promising application of effective and cold chain-independent vaccination against diseases.
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http://dx.doi.org/10.1021/acsami.1c00706DOI Listing
March 2021

Drug repurposing against breast cancer by integrating drug-exposure expression profiles and drug-drug links based on graph neural network.

Bioinformatics 2021 Mar 19. Epub 2021 Mar 19.

Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai 201203, China.

Motivation: Breast cancer is one of the leading causes of cancer deaths among women worldwide. It is necessary to develop new breast cancer drugs because of the shortcomings of existing therapies. The traditional discovery process is time-consuming and expensive. Repositioning of clinically approved drugs has emerged as a novel approach for breast cancer therapy. However, serendipitous or experiential repurposing cannot be used as a routine method.

Results: In this study, we proposed a graph neural network model GraphRepur based on GraphSAGE for drug repurposing against breast cancer. GraphRepur integrated two major classes of computational methods, drug network-based and drug signature-based. The differentially expressed genes of disease, drug-exposure gene expression data, and the drug-drug links information were collected. By extracting the drug signatures and topological structure information contained in the drug relationships, GraphRepur can predict new drugs for breast cancer, outperforming previous state-of-the-art approaches and some classic machine learning methods. The high-ranked drugs have indeed been reported as new uses for breast cancer treatment recently.

Availability: The source code of our model and datasets are available at: https://github.com/cckamy/GraphRepur and https://figshare.com/articles/software/GraphRepur_Breast_Cancer_Drug_Repurposing/14220050.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab191DOI Listing
March 2021

Identification and validation of an autophagy-related long non-coding RNA signature as a prognostic biomarker for patients with lung adenocarcinoma.

J Thorac Dis 2021 Feb;13(2):720-734

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: Lung adenocarcinoma (LUAD) is the most predominant pathological subtype of lung cancer, accounting for 40-70% of all lung cancer cases. Although significant improvements have been made in the screening, diagnosis, and precise management in recent years, the prognosis of LUAD remains bleak. This study aimed to investigate the prognostic significance of autophagy-related long non-coding RNAs (lncRNAs) and construct an autophagy-related lncRNA prognostic model in LUAD.

Methods: The gene expression data of LUAD patients were obtained from The Cancer Genome Atlas (TCGA) database. All autophagy-related genes were downloaded from the Human Autophagy Database (HADb). Spearman's correlation test was exploited to identify potential autophagy-related lncRNAs. The multivariate Cox regression analysis was used to construct the prognostic signature, which divided LUAD patients into high-risk and low-risk groups. Subsequently, the receiver operating characteristic (ROC) curves were generated to assess the predictive ability of this prognostic model for overall survival (OS) in these individuals. Then, the Gene set enrichment analysis (GSEA) was conducted to execute pathway enrichment analysis. Finally, a multidimensional validation was exploited to verify our findings.

Results: A total of 1,144 autophagy-related lncRNAs were identified to construct the co-expression network via Spearman's correlation test (|R| >0.4 and P≤0.001). Ultimately, a 16 autophagy-related lncRNAs prognostic model was constructed, and the area under the ROC curve (AUC) was 0.775. The results of GSEA enrichment analysis showed that the genes in the high-risk group were mainly enriched in cell cycle and p53 signaling pathways. The results of the multidimensional database validation indicated that the expression level of BIRC5 was significantly correlated with the expression level of TMPO-AS1. Furthermore, both TMPO-AS1 and BIRC5 had a higher expression level in LUAD samples. LUAD patients with high expression levels of TMPO-AS1 and BIRC5 were correlated with advanced disease stage and poor OS.

Conclusions: In summary, our results suggested that the prognostic signature of the 16 autophagy-related lncRNAs has significant prognostic value for LUAD patients. Furthermore, TMPO-AS1 and BIRC5 are potential predictors and therapeutic targets in these individuals.
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http://dx.doi.org/10.21037/jtd-20-2803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947511PMC
February 2021

Anoctamin 1 antagonism potentiates conventional tocolytic-mediated relaxation of pregnant human uterine smooth muscle.

J Physiol Sci 2021 Feb 22;71(1). Epub 2021 Feb 22.

Department of Anesthesiology, Columbia University College of Physicians and Surgeons, 622 W. 168th St. P&S Box 46, New York, NY, 10032, USA.

Background: Currently available tocolytic agents are not effective treatment for preterm labor beyond 48 h. A major reason is the development of maternal side effects which preclude the maintenance of an effective steady-state drug concentration. One strategy that can mitigate these side effects is utilizing synergistic drug combinations to reduce the drug concentrations necessary to elicit a clinical effect. We have previously shown that three anoctamin 1 (ANO1) antagonists mediate potent relaxation of precontracted human uterine smooth muscle (USM). In this study, we aimed to determine whether a combination of sub-relaxatory doses of tocolytic drugs in current clinical use [the L-type voltage-gated calcium channel (VGCC) blocker, nifedipine (NIF); and the β-adrenergic (β2AR) agonist, terbutaline (TRB)] will potentiate USM relaxation with two ANO1 antagonists [benzbromarone (BB) and MONNA (MN)].

Objective: This study sought to examine the synergistic potency and mechanistic basis of two ANO1 antagonists with currently available tocolytic drugs. Functional endpoints assessed included relaxation of pre-contracting pregnant human USM tissue, inhibition of intracellular calcium release, and reduction of spontaneous transient inward current (STIC) recordings in human uterine smooth muscle cells.

Methods: Human myometrial strips and primary human USM cells were used in organ bath and calcium flux experiments with different combinations of sub-threshold doses of ANO1 antagonists and terbutaline or nifedipine to determine if ANO1 antagonists potentiate tocolytic drugs.

Results: The combination of sub-threshold doses of two ANO1 antagonists and current tocolytic drugs demonstrate a significant degree of synergy to relax human pregnant USM compared to the effects achieved when these drugs are administered individually.

Conclusion: A combination of sub-threshold doses of VGCC blocker and β2AR agonist with ANO1 antagonists potentiates relaxation of oxytocin-induced contractility and calcium flux in human USM ex vivo. Our findings may serve as a foundation for novel tocolytic drug combinations.
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http://dx.doi.org/10.1186/s12576-021-00792-3DOI Listing
February 2021

Efficacy Analysis of Adjuvant Chemotherapy with Gemcitabine Plus Platinum or S-1 in Biliary Tract Carcinoma: A Multi-Center Retrospective Study.

Cancer Manag Res 2021 29;13:889-898. Epub 2021 Jan 29.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China.

Purpose: Biliary tract cancers (BTCs) have a poor overall prognosis, as patients who underwent curative surgery frequently experience disease recurrence. At present, there is a paucity of well-documented adjuvant chemotherapy regimen. This study aimed to assess whether gemcitabine plus platinum or S-1 adjuvant chemotherapy have different impact on relapse-free survival (RFS).

Patients And Methods: We selected patients undergoing radical biliary tract cancer surgery, pathologically confirmed adenocarcinoma and received gemcitabine plus platinum (cisplatin or oxaliplatin) or S-1 adjuvant chemotherapy from September 2013 to May 2020. The primary study endpoint was RFS. The secondary endpoint was safety.

Results: Overall 136 patients were enrolled. The median follow-up was 32.3 months and the median RFS was 17.0 months (95% CI 8.9-25.1). The median RFS was 14.1 months (95% CI 6.7-21.5) in gemcitabine plus platinum group and 33.0 months (95% CI 9.3-56.7) in gemcitabine plus S-1 (GS) group, a non-significant difference both in univariate (P=0.092) and in multivariate analysis (P=0.058). Lymph node status (N- vs N+: HR=0.477, 95% CI 0.285-0.799; P=0.005) and chemotherapy cycles (<6 vs 6-8: HR=1.828, 95% CI 1.117-2.993; P=0.016) were independent impact factors for RFS. GS group had lower incidence of adverse reactions.

Conclusion: Compared with gemcitabine plus platinum, GS regimen has a tendency to obtain longer RFS (although there is no statistically significant difference) and less toxic. GS regimen has the potential to be investigated as a standard regimen for adjuvant chemotherapy.
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http://dx.doi.org/10.2147/CMAR.S290083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853243PMC
January 2021

Optimal segmentation scale selection and evaluation of cultivated land objects based on high-resolution remote sensing images with spectral and texture features.

Environ Sci Pollut Res Int 2021 Jan 27. Epub 2021 Jan 27.

State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610065, China.

As the remote sensing technology develops, there are increasingly more kinds of remote sensing images available from different sensors. High-resolution remote sensing images are widely used in the detection of land cover/land change due to their plenty of characteristics of a specific feature in terms of spectrum, shape, and texture. Current studies regarding cultivated land resources that are the material basis for the human beings to survive and develop focus on the method to accurately obtain the quantity of cultivated land in a region and understand the conditions and the trend of change of the cultivated land. Pixel-based method and object-oriented method are the main methods to extract cultivated land in remote sensing field. Pixel-based method ignores high-level image information, while object-oriented method takes the image spot after image segmentation as the basic unit of information extraction, which can make full use of spectral features, spatial features, semantic features, and contextual features. Image segmentation is a key step of object-oriented method; the core problem is how to obtain the optimal segmentation scale. Traditional methods for determining the optimal segmentation scale of features (such as the homogeneity-heterogeneity method, the maximum area method, and the mean variance method), in which only the spectral and geometrical characteristics are considered, while the textural characteristics are neglected. Based on this, the Quickbird and unmanned aerial vehicle (UAV) images obtained in Xiyu Village, Pengzhou City, Sichuan Province, China, were selected as experimental objects, and the texture mean and spectral grayscale mean method (MANC method based on GLCM), which comprehensively considered the spectrum, shape, and texture features, was proposed to calculate the optimal segmentation scale of cultivated land in the study area. The error segment index (ESI) and centroids distance index (CDI) were adopted to evaluate image segmentation quality based on the method of area and position differences. The experimental results show that the MANC method based on GLCM can obtain higher segmentation precision than the traditional methods, and the segmentation results are in good agreement with the cultivated land boundary obtained by visual interpretation.
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http://dx.doi.org/10.1007/s11356-021-12552-2DOI Listing
January 2021

Bias Stress Stability of Solution-Processed Nano Indium Oxide Thin Film Transistor.

Micromachines (Basel) 2021 Jan 22;12(2). Epub 2021 Jan 22.

State Key Laboratory of Luminescent Materials and Devices, Institute of Polymer Optoelectronic Materials and Devices, South China University of Technology, Guangzhou 510640, China.

In this paper, the effects of annealing temperature and other process parameters on spin-coated indium oxide thin film transistors (InO-TFTs) were studied. The research shows that plasma pretreatment of glass substrate can improve the hydrophilicity of glass substrate and stability of the spin-coating process. With Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis, it is found that InO thin films prepared by the spin coating method are amorphous, and have little organic residue when the annealing temperature ranges from 200 to 300 °C. After optimizing process conditions with the spin-coated rotating speed of 4000 rpm and the annealing temperature of 275 °C, the performance of InO-TFTs is best (average mobility of 1.288 cm·V·s, I/I of 5.93 × 10, and SS of 0.84 V·dec). Finally, the stability of InO-TFTs prepared at different annealing temperatures was analyzed by energy band theory, and we identified that the elimination of residual hydroxyl groups was the key influencing factor. Our results provide a useful reference for high-performance metal oxide semiconductor TFTs prepared by the solution method.
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http://dx.doi.org/10.3390/mi12020111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7911419PMC
January 2021

Temperature-Controlled Crystal Size of Wide Band Gap Nickel Oxide and Its Application in Electrochromism.

Micromachines (Basel) 2021 Jan 14;12(1). Epub 2021 Jan 14.

State Key Laboratory of Luminescent Materials and Devices, Institute of Polymer Optoelectronic Materials and Devices, South China University of Technology, Guangzhou 510640, China.

Nickel oxide (NiO) is a wide band gap semiconductor material that is used as an electrochromic layer or an ion storage layer in electrochromic devices. In this work, the effect of annealing temperature on sol-gel NiO films was investigated. Fourier transform infrared spectroscopy (FTIR) showed that the formation of NiO via decomposition of the precursor nickel acetate occurred at about 300 °C. Meanwhile, an increase in roughness was observed by Atomic force microscope (AFM), and precipitation of a large number of crystallites was observed at 500 °C. X-ray Diffraction (XRD) showed that the NiO film obtained at such a temperature showed a degree of crystallinity. The film crystallinity and crystallite size also increased with increasing annealing temperature. An ultraviolet spectrophotometer was used to investigate the optical band gap of the colored NiO films, and it was found that the band gap increased from 3.65 eV to 3.74 eV with the increase in annealing temperature. An electrochromic test further showed that optical modulation density and coloring efficiency decreased with the increase in crystallite size. The electrochromic reaction of the nickel oxide film is more likely to occur at the crystal interface and is closely related to the change of the optical band gap. An NiO film with smaller crystallite size is more conducive to ion implantation and the films treated at 300 °C exhibit optimum electrochromic behavior.
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http://dx.doi.org/10.3390/mi12010080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828803PMC
January 2021

The spatial variation of soil bacterial community assembly processes affects the accuracy of source tracking in ten major Chinese cities.

Sci China Life Sci 2021 Jan 8. Epub 2021 Jan 8.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.

Urban soils harbor billions of bacterial cells and millions of species. However, the distribution patterns and assembly processes of bacterial communities remain largely uncharacterized in urban soils. It is also unknown if we can use the bacteria to track soil sources to certain cities and districts. Here, Illumina MiSeq sequencing was used to survey soil bacterial communities from 529 random plots spanning 61 districts and 10 major cities in China. Over a 3,000 km range, community similarity declined with increasing geographic distance (Mantel r=0.62), and community composition was clustered by city (R=0.50). Within cities (<100 km), the aforementioned biogeographic patterns were weakened. Process analysis showed that homogenizing dispersal and dispersal limitation dominated soil bacterial assembly at small and large spatial scales, respectively. Accordingly, the probabilities of accurately tracking random soil sources to certain cities and districts were 90.0% and 66.7%, respectively. When the tested samples originated from cities that were more than 1,265 km apart, the soil sources could be identified with nearly 100% accuracy. Overall, this study demonstrates the strong distance-decay relationship and the clear geographic zoning of urban soil bacterial communities among cities. The varied importance of different community assembly processes at multiple spatial scales strongly affects the accuracy of microbial source tracking.
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http://dx.doi.org/10.1007/s11427-020-1843-6DOI Listing
January 2021

Comparison of loop extrusion and diffusion capture as mitotic chromosome formation pathways in fission yeast.

Nucleic Acids Res 2021 02;49(3):1294-1312

Chromosome Segregation Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Underlying higher order chromatin organization are Structural Maintenance of Chromosomes (SMC) complexes, large protein rings that entrap DNA. The molecular mechanism by which SMC complexes organize chromatin is as yet incompletely understood. Two prominent models posit that SMC complexes actively extrude DNA loops (loop extrusion), or that they sequentially entrap two DNAs that come into proximity by Brownian motion (diffusion capture). To explore the implications of these two mechanisms, we perform biophysical simulations of a 3.76 Mb-long chromatin chain, the size of the long Schizosaccharomyces pombe chromosome I left arm. On it, the SMC complex condensin is modeled to perform loop extrusion or diffusion capture. We then compare computational to experimental observations of mitotic chromosome formation. Both loop extrusion and diffusion capture can result in native-like contact probability distributions. In addition, the diffusion capture model more readily recapitulates mitotic chromosome axis shortening and chromatin compaction. Diffusion capture can also explain why mitotic chromatin shows reduced, as well as more anisotropic, movements, features that lack support from loop extrusion. The condensin distribution within mitotic chromosomes, visualized by stochastic optical reconstruction microscopy (STORM), shows clustering predicted from diffusion capture. Our results inform the evaluation of current models of mitotic chromosome formation.
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http://dx.doi.org/10.1093/nar/gkaa1270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897502PMC
February 2021

The Construction and Analysis of Tumor-Infiltrating Immune Cells and ceRNA Networks in Bladder Cancer.

Front Genet 2020 18;11:605767. Epub 2020 Dec 18.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: Bladder cancer (BLCA) is the 11th most common malignancy worldwide. Although significant improvements have been made in screening, diagnosis, and precise management in recent years, the prognosis of BLCA remains bleak.

Objectives: This study aimed to investigate the prognostic significance of tumor-infiltrating immune cells and construct ceRNA networks in BLCA patients.

Methods: The expression data of BLCA patients were obtained from The Cancer Genome Atlas (TCGA) database. A competing endogenous RNA (ceRNA) network was constructed to identify the hub genes involved in the prognosis of BLCA. The CIBERSORT algorithm was utilized to investigate the infiltration levels of 22 subsets of immune cells. Ultimately, the nomogram was generated to visualize the survival probability of each patient, with the calibration curve being performed to assess its performance. Furthermore, the Pearson correlation test was used to explore the correlation between the identified hub genes in the ceRNA network and the prognostic-related immune cells.

Results: A total of eight elements in the ceRNA network were considered as key members and correlated with the prognosis of BLCA, including , , , , , , , and . T cells CD8, T cells follicular helper (Tfh), and neutrophils were identified as independent prognostic factors in BLCA. The co-expression analysis showed that there was a significant correlation between the identified hub genes and immune cells.

Conclusion: Our results suggest that the mechanism of regulates the expression of and , and the infiltration of Tfh and neutrophils might play pivotal roles in the progression of BLCA.
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http://dx.doi.org/10.3389/fgene.2020.605767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775311PMC
December 2020

Tumor Mutation Burden, Immune Cell Infiltration, and Construction of Immune-Related Genes Prognostic Model in Head and Neck Cancer.

Int J Med Sci 2021 1;18(1):226-238. Epub 2021 Jan 1.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common malignancy worldwide, and the prognosis of HNSCC remains bleak. Numerous studies revealed that the tumor mutation burden (TMB) could predict the survival outcomes of a variety of tumors. This study aimed to investigate the TMB and immune cell infiltration in these patients and construct an immune-related genes (IRGs) prognostic model. The expression data of 546 HNSCC patients were obtained from The Cancer Genome Atlas (TCGA) database. All patients were divided into high- and low- TMB groups, and the relationship between TMB and clinical relevance was further analyzed. The differentially expressed genes (DEGs) were identified using the R software package, limma. Functional enrichment analyses were conducted to identify the significantly enriched pathways between two groups. CIBERSORT algorithm was adopted to calculate the abundance of 22 leukocyte subtypes. The IRGs prognostic model was constructed via the multivariate Cox regression analysis. Missense mutation and single nucleotide variants (SNV) were the most predominant mutation types in HNSCC. , , and were the most frequently mutated genes. Patients with high TMB were observed with worse survival outcomes. The functional analysis of TMB associated DEGs showed that the identified DEGs mainly involved in spliceosome, RNA degradation, proteasome, and RNA polymerase pathways. We observed that macrophages, T cells CD8, and T cells CD4 memory were the most commonly infiltrated subtypes of immune cells in HNSCC. Finally, an IRGs prognostic model was constructed, and the AUC of the ROC curve was 0.635. Our results suggest that high TMB is associated with poor prognosis in HNSCC patients. The constructed model has potential prognostic value for the prognosis of these individuals, and it needs to be further validated in large-scale and prospective studies.
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http://dx.doi.org/10.7150/ijms.51064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738958PMC
January 2021

LINC00476 Suppresses the Progression of Non-Small Cell Lung Cancer by Inducing the Ubiquitination of SETDB1.

Radiat Res 2021 03;195(3):275-283

Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou; 450000, China.

Long non-coding RNAs are involved in the tumorigenesis of non-small cell lung cancer (NSCLC). Here we investigated whether LINC00476 affects the proliferation, invasion and migration of NSCLC cells via the SETDB1-activated Wnt/β-catenin pathway. The expression of LINC00476, SETDB1, Wnt1 and β-catenin were determined in NSCLC tumor tissues and the paired adjacent tissues, as well as in NSCLC cell lines and bronchial epithelioid cell lines. Cell proliferation, invasion and migration were determined using cell counting kit-8 assay and transwell assay. The relationship between LINC00476 and SETDB1 was elucidated using RNA pull-down, RNA immunoprecipitation and ubiquitination assays. LINC00476 was found to be significantly downregulated, while SETDB1, Wnt1 and β-catenin were upregulated in NSCLC tumor tissues and cell lines compared to the normal ones. Overexpression of LINC00476 promoted the proliferation, invasion and migration of NSCLC cells, and suppressed tumor growth in the mouse xenograft. Meanwhile, overexpression of LINC00476 induced the degradation of SETDB1 by promoting its ubiquitination. The simultaneous overexpression of LINC00476 and SETDB1 negated the inhibition of LINC00476 overexpression on the proliferation, invasion and migration of NSCLC cells. In conclusion, these findings indicate that LINC00476 acts as a tumor suppressor in NSCLC by downregulating SETDB1, which provides a novel target in the treatment of NSCLC.
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http://dx.doi.org/10.1667/RADE-20-00105.1DOI Listing
March 2021

A computational model of liver tissue damage and repair.

PLoS One 2020 21;15(12):e0243451. Epub 2020 Dec 21.

Biocomplexity Institute, Indiana University, Bloomington, IN, United States of America.

Drug induced liver injury (DILI) and cell death can result from oxidative stress in hepatocytes. An initial pattern of centrilobular damage in the APAP model of DILI is amplified by communication from stressed cells and immune system activation. While hepatocyte proliferation counters cell loss, high doses are still lethal to the tissue. To understand the progression of disease from the initial damage to tissue recovery or death, we computationally model the competing biological processes of hepatocyte proliferation, necrosis and injury propagation. We parametrize timescales of proliferation (α), conversion of healthy to stressed cells (β) and further sensitization of stressed cells towards necrotic pathways (γ) and model them on a Cellular Automaton (CA) based grid of lattice sites. 1D simulations show that a small α/β (fast proliferation), combined with a large γ/β (slow death) have the lowest probabilities of tissue survival. At large α/β, tissue fate can be described by a critical γ/β* ratio alone; this value is dependent on the initial amount of damage and proportional to the tissue size N. Additionally, the 1D model predicts a minimum healthy population size below which damage is irreversible. Finally, we compare 1D and 2D phase spaces and discuss outcomes of bistability where either survival or death is possible, and of coexistence where simulated tissue never completely recovers or dies but persists as a mixture of healthy, stressed and necrotic cells. In conclusion, our model sheds light on the evolution of tissue damage or recovery and predicts potential for divergent fates given different rates of proliferation, necrosis, and injury propagation.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0243451PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7752149PMC
January 2021

Vorapaxar-modified polysulfone membrane with high hemocompatibility inhibits thrombosis.

Mater Sci Eng C Mater Biol Appl 2021 Jan 11;118:111508. Epub 2020 Sep 11.

Department of Hematology, Xiangya Hemophilia Diagnosis and Treatment Center, Xiangya Hospital, Central South University, China.

Hemodialysis therapy is intended for patients suffering from renal insufficiency, pancreatitis, and other serious diseases. Platelets are an important active ingredient in the thrombosis induced by hemodialysis membranes. So far, there are few studies of hemodialysis membranes focusing on the effects of protease-activated receptor 1 (PAR1) activation on the platelet membrane. Among various antithrombotic agents, vorapaxar is a novel PAR1 inhibitor with high efficacy. In this study, we constructed a vorapaxar-modified polysulfone (VMPSf) membrane using immersion-precipitation phase transformation methods and characterized the microstructure in terms of hydrophilicity and mechanical properties. The water contact angle of the VMPSf membrane was 22.45% lower than that of the PSf membrane. A focused determination of platelet morphology was obtained using scanning electron microscopy. Meanwhile, we evaluated the effects of a VMPSf membrane on platelet adhesion. We observed that the VMPSf membrane could reduce the number of adhered platelets without altering their spherical or elliptical shape. The PAR1 levels in VMPSf membranes were 7.4 MFI lower than those in PSf membranes, suggesting that this modified membrane can effectively inhibit platelet activation. Activated partial thromboplastin time (APTT, 5.3 s extension) and thrombin time (TT, 2.1 s extension) reflect good anticoagulant properties. Recalcification time (80.6 s extension) and fibrinogen adsorption (9.9 μg/cm reduction) were related to antithrombotic properties. To determine the biosafety of VMPSf membranes, we investigated antianaphylactic and anti-inflammatory properties in vitro and acute toxicity in vivo, it was obvious that C3a and C5a had decreased to 9.6 and 0.8 ng/mL, respectively. The results indicated that the VMPSf membrane has potential for clinical application.
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http://dx.doi.org/10.1016/j.msec.2020.111508DOI Listing
January 2021

Higher content of microcystin-leucine-arginine promotes the survival of intrahepatic cholangiocarcinoma cells via regulating SET resulting in the poorer prognosis of patients.

Cell Prolif 2021 Feb 25;54(2):e12961. Epub 2020 Nov 25.

Department of Hepatopancreatobiliary Surgery, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China.

Background & Aims: Intrahepatic cholangiocarcinoma (ICC) has over the last 10 years become the focus of increasing concern largely due to its rising incidence and high mortality rates worldwide. Microcystin-leucine-arginine (MC-LR) has been reported to be carcinogenic, but there are no data on the linkage between MC-LR and ICC. This study aimed to explore whether the content levels of MC-LR in the tumour tissues of ICC patients be associated with the prognosis and if so, to characterize the mechanism in ICC cells.

Methods: We conducted a retrospective study to evaluate the prognostic value of MC-LR in ICC after resection. All patients were divided into two groups according to the content of MC-LR in tumour via immunohistochemistry: low-MC-LR group (n = 28) and high-MC-LR group (n = 30).

Results: Multivariate analysis showed high-MC-LR level was the prognostic factor for OS and RFS after hepatectomy (P = .011 and .044). We demonstrated that MC-LR could promote the survival of human ICC cell lines and SET was identified as an important mRNA in the progression via RNA array.

Conclusions: We provide evidence that MC-LR was an independent prognostic factor for ICC in humans by modulating the expression of SET in human ICC cells.
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http://dx.doi.org/10.1111/cpr.12961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848955PMC
February 2021

Clinical outcomes and prognostic factors in bloodstream infections due to extended-spectrum β-lactamase-producing Enterobacteriaceae among patients with malignancy: a meta-analysis.

Ann Clin Microbiol Antimicrob 2020 Nov 23;19(1):53. Epub 2020 Nov 23.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, People's Republic of China.

Background: The colonization of Extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) in bloodstream infections (BSIs) has been increased dramatically worldwide, and it was associated with worse clinical outcomes in patients with malignancy. We performed the meta-analysis to investigate the prognosis and risk factors in BSIs caused by ESBL-PE in oncological patients.

Methods: PubMed, EMBASE, and Cochrane Library were searched for related studies. All-cause mortality was considered as the primary outcome. Subgroup analyses, meta-regression analyses, and sensitivity analysis were used to investigate heterogeneity and reliability in results.

Results: 6,729 patients from 25 studies were eligible. Six studies enrolled oncological patients with BSIs caused by ESBL-PE only, while 19 studies both enrolled ESBL-PE and non-ESBL-PE infections. The results showed that BSIs caused by ESBL-PE in patients with malignancy was associated with higher mortality than non-ESBL-PE infections (RR = 2.21, 95% CI: 1.60-3.06, P < 0.001), with a significant between-study heterogeneity (I =78.3%, P < 0.001). Subgroup analyses showed that children (RR = 2.80, 95% CI: 2.29-3.43, P < 0.001) and hematological malignancy (RR = 3.20, 95% CI: 2.54-4.03, P < 0.001) were associated with a higher mortality. Severe sepsis/ septic shock, pneumonia, and ICU admission were the most common predictors of mortality.

Conclusions: Our study identified that BSIs caused by ESBL-PE in patients with malignancy were associated with worse clinical outcomes compared with non-ESBL-PE infections. Furthermore, children and hematological malignancy were associated with higher mortality. Severe sepsis/ septic shock, pneumonia, and ICU admission were the most common predictors of mortality.
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http://dx.doi.org/10.1186/s12941-020-00395-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685587PMC
November 2020

Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance.

Environ Sci Technol 2020 12 15;54(23):15366-15375. Epub 2020 Nov 15.

Department of Biological Systems Engineering, Washington State University, Pullman, Washington 99164, United States.

This study explored the possibility of incorporating extremophilic algal cultivation into dairy wastewater treatment by characterizing a unique algal strain. Results showed that extremophilic microalgae CA1 newly isolated from dairy wastewater tolerated a high level of ammonia nitrogen (2.7 g/L), which was over 20 times the ammonia nitrogen that regular sp. could tolerate. The isolate was mixotrophically cultured in dairy effluent treated by anaerobic digestion (AD) for recycling nutrients and polishing the wastewater. The highest biomass content of 13.3 g/L and protein content of 43.4% were achieved in the culture in AD effluent. Up to 96% of the total nitrogen and 79% of the total phosphorus were removed from the dairy AD effluent. The ability of the algae to tolerate a high level of ammonia nitrogen suggests the potential for direct nutrient recycling from dairy wastewater while producing algal biomass and high value bioproducts.
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http://dx.doi.org/10.1021/acs.est.0c02833DOI Listing
December 2020

PD-L1 Predicts Poor Prognosis in Surgically Resected Limited Stage Small-Cell Lung Cancer.

Cancer Manag Res 2020 30;12:10939-10948. Epub 2020 Oct 30.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, People's Republic of China.

Purpose: Small-cell lung cancer (SCLC) is an aggressive high-grade neuroendocrine tumor with limited treatment strategies. Programmed death 1 (PD-1) and its ligand (PD-L1), delta-like ligand-3 (DLL-3), and poly ADP-ribose polymerase (PARP) inhibitors have shed light on the treatment of extensive stage-SCLC. However, the expression and prognostic role of PD-L1, DLL-3, and PARP are barely explored in surgically resected limited stage-SCLC (LS-SCLC).

Methods: We retrospectively reviewed 404 SCLC patients from 2011 to 2018 in the First Affiliated Hospital of Xi'an Jiaotong University and collected 43 surgically resected LS-SCLC samples with adequate materials and histological specimens containing abundant tumor cells. Immunohistochemistry staining of PD-L1, DLL-3, and PAPR1 was performed by anti-PD-L1 (22C3/Dako), anti-DLL-3, and anti-PAPR1 antibodies, respectively. Positive expression of PD-L1 was characterized as >5% tumor cells and/or tumor-infiltrating immune cells expressing PD-L1. The correlation between PD-L1, DLL-3, PARP1, and clinicopathological characteristics of surgically resected LS-SCLC patients was performed by χ test. The survival curves were calculated by the Kaplan-Meier method and analyzed by the Log rank test and Cox proportional hazards model.

Results And Conclusion: 63.04% patients were positive for PD-L1, 65.12% were positive for DLL-3, and 20.93% were positive for PARP1. DLL-3 was significantly overexpressed in SCLC tissues, compared with matched para-noncancerous tissues. Male, elder than 60 years old, advanced TNM stage, smoking, and positive PD-L1 expression predicted shorter DFS, while patients received adjuvant therapy performed better DFS. Further multivariate analysis revealed that TNM stage (HR=2.51, 95% CI=1.31-4.78, =0.005) was an individual prognostic factor for DFS in LS-SCLC. Moreover, advanced TNM stage and positive PD-L1 expression also indicated worse OS, but adjuvant therapy improved OS in LS-SCLC. Multivariate analysis demonstrated that PD-L1 and TNM stage were independent and significant negative predictive factors for OS (HR=2.89, 95% CI=1.21-6.93, =0.017; HR=2.49, 95% CI=1.25-4.94, =0.009 for PD-L1 and TNM stage, respectively), while adjuvant treatment was an independent positive prognostic factor for OS (HR=0.37, 95% CI=0.17-0.81, =0.012).
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http://dx.doi.org/10.2147/CMAR.S260599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608588PMC
October 2020

Fission yeast condensin contributes to interphase chromatin organization and prevents transcription-coupled DNA damage.

Genome Biol 2020 11 5;21(1):272. Epub 2020 Nov 5.

Chromosome Segregation Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK.

Background: Structural maintenance of chromosomes (SMC) complexes are central organizers of chromatin architecture throughout the cell cycle. The SMC family member condensin is best known for establishing long-range chromatin interactions in mitosis. These compact chromatin and create mechanically stable chromosomes. How condensin contributes to chromatin organization in interphase is less well understood.

Results: Here, we use efficient conditional depletion of fission yeast condensin to determine its contribution to interphase chromatin organization. We deplete condensin in G2-arrested cells to preempt confounding effects from cell cycle progression without condensin. Genome-wide chromatin interaction mapping, using Hi-C, reveals condensin-mediated chromatin interactions in interphase that are qualitatively similar to those observed in mitosis, but quantitatively far less prevalent. Despite their low abundance, chromatin mobility tracking shows that condensin markedly confines interphase chromatin movements. Without condensin, chromatin behaves as an unconstrained Rouse polymer with excluded volume, while condensin constrains its mobility. Unexpectedly, we find that condensin is required during interphase to prevent ongoing transcription from eliciting a DNA damage response.

Conclusions: In addition to establishing mitotic chromosome architecture, condensin-mediated long-range chromatin interactions contribute to shaping chromatin organization in interphase. The resulting structure confines chromatin mobility and protects the genome from transcription-induced DNA damage. This adds to the important roles of condensin in maintaining chromosome stability.
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http://dx.doi.org/10.1186/s13059-020-02183-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643427PMC
November 2020

A Potential Biomarker of Combination of Tumor Mutation Burden and Copy Number Alteration for Efficacy of Immunotherapy in -Mutant Advanced Lung Adenocarcinoma.

Front Oncol 2020 24;10:559896. Epub 2020 Sep 24.

Department of Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Objectives: The () mutation is the commonest oncogenic drive mutation in lung adenocarcinoma (LUAD) and immunotherapy may be quite promising for -mutant LUAD. While the effects of tumor mutation burden (TMB) and copy number alteration (CNA) are poorly understood in this illness, our study aimed to explore the roles TMB and CNA play in the prediction of response to immune checkpoint inhibitor (ICI) therapy in advanced -mutant LUAD.

Methods: Mutation and clinical data were downloaded from cBioPortal. We evaluated mutation status and divided patients into different subgroups based on TMB and CNA cutoffs to investigate the predictive value of these biomarkers on ICI response.

Results: mutation with concurrent or mutations had higher TMB and CNA compared to mutation alone. The G12C and G > T mutation subgroups, with or co-mutation, also had higher TMB and CNA. We found that TMB and CNA were independently associated with progression-free survival (PFS) and durable clinical benefits (DCB); TMB was positively correlated with PFS ( = 0.0074) and DCB ( = 0.0008) while low CNA was associated with prolonged PFS ( = 0.0060) and DCB ( = 0.0018). However, TMB alone did not distinguish benefits among -mutant patients. Notably, when combining TMB and CNA, low TMB and high CNA revealed worse outcomes of ICI therapy (mPFS: 2.20m, = 0.0023; proportion of DCB: 24%, = 0.0001).

Conclusion: The combination of TMB and CNA provides more sensible and accurate prediction of ICI response than individual factors in -mutant LUAD. Moreover, low TMB and high CNA can be utilized as a potential biomarker to predict adverse outcome in -mutant LUAD.
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http://dx.doi.org/10.3389/fonc.2020.559896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541961PMC
September 2020

How Can Manufacturers Promote Green Innovation in Food Supply Chain? Cost Sharing Strategy for Supplier Motivation.

Front Psychol 2020 18;11:574832. Epub 2020 Sep 18.

Department of Leisure and Sport Management, Cheng Shiu University, Kaohsiung, China.

In the innovation of production activities by green product manufacturing or application, food supply chain cooperation is an important method to optimize the allocation of internal and external innovation resources, strengthen their own core capabilities and achieve sustainable development of enterprises. Whether the traditional revenue sharing or cost sharing strategy is still efficient in the food supply chain cooperation aiming at green innovation attracts a lot of attention. Further research about whether the traditional cooperation contract can effectively motivate suppliers to maximize their innovation efforts is required. In this paper, the green innovation effort level parameters are designed and the constraint factor of the green preference of consumers at the market end is applied to discuss the incentive strategy of cost sharing led by manufacturers. Stackelberg equilibrium structure is utilized in the incentive model in this paper to discuss the existence of the optimal cost sharing ratio, the optimal effort level and the optimal income of green innovation cooperation in the food supply chain. The results show that when the supply is interrupted due to the insufficient stimulation of green consumption at the market demand side, manufacturers need to stimulate their green innovation efforts by sharing the cost of suppliers, and the cost sharing proportion is affected by the marginal profit coefficient of manufacturers and suppliers. When the relationship between the marginal profit of suppliers and the marginal profit of manufacturers reaches a certain threshold, manufacturers use the cost sharing contract, which can effectively stimulate the green innovation efforts of suppliers and optimize the overall income of the food supply chain.
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http://dx.doi.org/10.3389/fpsyg.2020.574832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533551PMC
September 2020

[Effects of hypothermia on the repolarization duration of ventricular myocytes in rats and its mechanism].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2020 May;36(3):228-231

School of Anesthesiology, Guizhou Medical University, Guiyang 550001.

To observe the effects of hypothermia on the repolarization duration and the expression of Kir2.1 protein of ventricular myocytes in isolated rat heart and explore the role of Kir2.1 protein. Eighteen healthy adult male Sprague-Dawley rats were randomly divided into three groups (n=6 per group): Control group (C group), 35℃ group (H group), 32℃ group (H group). Langendorff isolated heart models were established. After 15 min 37℃ K-H fluid banlanced perfusion, C group continued to perfuse the K-H solution at 37℃ for 30 minutes, H group continued to perfuse the K-H solution at 35℃ for 30 minutes, H group continued to perfuse the K-H solution at 32℃ for 30 minutes. At 15 min of balanced perfusion (T), and 30 min of continuous perfusion (T), the heart rate,and the MAP in the three layers of the left ventricular anterior wall were recorded, the action potential duration at 50% repolarization (MAPD), the action potential duration at 90% repolarization (MAPD) and transmural dispersion of repolarization(TDR) were calculated. At the same time, the occurrence of arrhythmia was recorded. The expression of Kir2.1 protein was measured by Western blot. The average optical density (AOD) and the distribution of Kir2.1 protein were measured by immunohistochemistry in the ventricular tissue measured by electrophysiology. Compared with T, the heart rate was decreased, MAPD and MAPD were prolonged significantly (P<0.05), and TDR was increased significantly (P<0.05) in H group, H group at T. Compared with C group, the HR was decreased, the MAPD was prolonged significantly (P<0.05), TDR was increased significantly (P<0.05),the expression and the AOD of Kir2.1 protein were decreased significantly (P<0.05) in Hgroup, Hgroup at T. Compared with H group, the heart rate of H group was decreased significantly (P<0.05), MAPD and MAPD were prolonged significantly (P<0.05), and TDR was increased significantly (P<0.05) at T. The distribution of Kir2.1 protein in group C was normal, while the distribution of Kir2.1 in H group and H group was disordered. Hypothermia prolonged the ventricular duration of repolarization and increased the dispersion of repolarization. The mechanism is related to the down-regulation the expression of Kir2.1 protein and the disorder of the distribution of Kir2.1 protein.
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http://dx.doi.org/10.12047/j.cjap.5914.2020.050DOI Listing
May 2020

A Novel Time Delay Estimation Algorithm for 5G Vehicle Positioning in Urban Canyon Environments.

Sensors (Basel) 2020 Sep 11;20(18). Epub 2020 Sep 11.

School of Electronic Engineering, Beijing University of Posts and Telecommunications, Beijing 100876, China.

Vehicle positioning with 5G can effectively compensate for the lack of vehicle positioning based on GNSS (Global Navigation Satellite System) in urban canyons. However, there is also a large ranging error in the non-line of sight (NLOS) propagation of 5G. Aiming to solve this problem, we consider a new time delay estimation algorithm called non-line of sight cancellation multiple signal classification (NC-MUSIC). This algorithm uses cross-correlation to identify and cancel the NLOS signal. Then, an unsupervised multipath estimation method is used to estimate the number of multipaths and extract the noise subspace. The MUSIC spectral function can be calculated by the noise subspace. Finally, the time delay of the direct path is estimated by searching the peak of MUSIC spectral function. This paper adopts the 5G channel model developed by 3GPP TR38.901 for simulation experiments. The experiment results demonstrated that the proposed algorithm has obvious advantages in terms of NLOS propagation for urban canyon environments. It provided a high-precision time delay estimation algorithm for observed time difference of arrival (OTDOA), joint angle of arrival (AOA) ranging, and other positioning methods in the 5G vehicle positioning method, which can effectively improve the positioning accuracy of 5G vehicle positioning in urban canyon environments.
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http://dx.doi.org/10.3390/s20185190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571156PMC
September 2020

De novo biosynthesis of linalool from glucose in engineered Escherichia coli.

Enzyme Microb Technol 2020 Oct 5;140:109614. Epub 2020 Jun 5.

Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Jiangxi Province, Ganzhou 341000, China. Electronic address:

Linalool is an important terpenoids of floral scents and has wide applications. In the past, several groups reported on a strategy to establish biosynthesis of linalool in yeast based on co-expression of Saccharomyces cerevisiae farnesyl diphosphate synthase ERG20 and Actinidia arguta linalool synthase LIS. However, ERG20 has both geranyl diphosphate synthase and farnesyl diphosphate synthase activities, which can lead to metabolic flow to farnesyl diphosphate. In this study, a heterologous linalool biosynthesis pathway was constructed in Escherichia coli and showed that using Abies grandis geranyl diphosphate synthase GPPS2 instead of ERG20 can effectively improve linalool biosynthesis. Subsequently, we further improved the biosynthesis of linalool by overexpression of isopentenyl diphosphate isomerase Idi.
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http://dx.doi.org/10.1016/j.enzmictec.2020.109614DOI Listing
October 2020

A blockchain-based scheme for privacy-preserving and secure sharing of medical data.

Comput Secur 2020 Dec 1;99:102010. Epub 2020 Sep 1.

School of Computer Science, Nanjing University of Posts and Telecommunications, Nanjing 210023, Jiangsu China.

How to alleviate the contradiction between the patient's privacy and the research or commercial demands of health data has become the challenging problem of intelligent medical system with the exponential increase of medical data. In this paper, a blockchain-based privacy-preserving scheme is proposed, which realizes secure sharing of medical data between several entities involved patients, research institutions and semi-trusted cloud servers. And meanwhile, it achieves the data availability and consistency between patients and research institutions, where zero-knowledge proof is employed to verify whether the patient's medical data meets the specific requirements proposed by research institutions without revealing patients' privacy, and then the proxy re-encryption technology is adopted to ensure that research institutions can decrypt the intermediary ciphertext. In addition, this proposal can execute distributed consensus based on PBFT algorithm for transactions between patients and research institutions according to the prearranged terms. Theoretical analysis shows the proposed scheme can satisfy security and privacy requirements such as confidentiality, integrity and availability, as well as performance evaluation demonstrates it is feasible and efficient in contrast with other typical schemes.
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http://dx.doi.org/10.1016/j.cose.2020.102010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462555PMC
December 2020