Publications by authors named "Xianxiao Huang"

5 Publications

  • Page 1 of 1

Effects of germination on the nutritional properties, phenolic profiles, and antioxidant activities of buckwheat.

J Food Sci 2015 May 9;80(5):H1111-9. Epub 2015 Apr 9.

Wilmar (Shanghai) Biotechnology Research and Development Center Co., Ltd, No. 118 Gaodong Rd., Pudong New District, Shanghai, 200137, China.

Germination is considered to be an effective process for improving the nutritional quality and functionality of cereals. In this study, changes of nutritional ingredients, antinutritional components, chemical composition, and antioxidant activities of buckwheat seeds over 72 h of germination were investigated, and the reasons for these changes are discussed. With the prolonged germination time, the contents of crude protein, reducing sugar, total phenolics, total flavonoids, and condensed tannins increased significantly, while the levels of crude fat, phytic acid, and the activity of trypsin inhibitor decreased. Phenolic compounds, such as rutin, vitexin, isovitexin, orientin, isoorientin, chlorogenic acid, trans-3-hydroxycinnamic acid, and p-hydroxybenzoic acid increased significantly during the germination process, which may be due to the activation of phenylalanine ammonialyase. The improvement of flavonoids led to significant enhancement of the antioxidant activities of germinated buckwheat. Germinated buckwheat had better nutritional value and antioxidant activities than ungerminated buckwheat, and it represented an excellent natural source of flavonoids and phenolic compounds, especially rutin and C-glycosylflavones. Therefore, germinated buckwheat could be used as a promising functional food for health promotion.
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http://dx.doi.org/10.1111/1750-3841.12830DOI Listing
May 2015

[Flavone and steroid chemical constituents from rhizome of Paris axialis].

Zhongguo Zhong Yao Za Zhi 2010 Nov;35(22):2994-8

School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.

Objective: To study the chemical constituents from the rhizome of Paris axialis.

Method: The compounds were isolated by column chromatography with silica gel and purified by Sephadex LH-20 column chromatography and preparative RP-HPLC. The structures were identified by means of spectroscopic methods.

Result: Fourteen compounds were isolated from the EtOAc extract and the n-BuOH extract of P. axialis. Their structures were identified as daucosterol (1), stigmasterol-3-O-beta-D-glycopyranoside (2), beta-ecdysterone (3), pennogenin-3-O-alpha-L-arabinofuranosyl (1 --> 4) -[alpha-L -rhamnopyranosyl (1 --> 2)] -beta-D-glycopyranoside (4), diosgenin-3-O-alpha-L- rhamnopy-ranosyl (1 --> 4) -alpha-L-rhamnopyranosyl (1 --> 4) [alpha-L-rhamnopyranosyl (1 --> 2)] -beta-D-glycopyranoside (5), kaempferol (6), rutin (7), myrincitrin (8), 4, 2', 4'-trihydroxychalcone (9), isorhamnetin-3-O-beta-D- glycopyranoside (10), isorhamnetin-3-O-alpha-L-rhamnopyranosyl (1 --> 2) -beta-D-glycopyranoside (11), isorhamnetin-3-O-beta-D-glucpyranosyl (1 --> 6) -beta-D-glycopyranoside (12), kayaflavone (13), amentoflavone (14).

Conclusion: Compounds 1-3 and 6-14 are isolated from P. axialis for the first time; and compounds 7-10, 13, 14 are isolated from the genus Paris for the first time.
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November 2010

[Numerical taxonamy of Paris plants].

Zhongguo Zhong Yao Za Zhi 2010 Jun;35(12):1518-20

School of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

Numerical taxonomic studies were carried out in order to elucidate the taxonomic relationship among 17 species belonging to Paris. Eighteen characters including 10 morphological, 4 pollen morphological, 2 cytotalonomical and 2 habitat characters were used for the analysis. On basis of UPGMA clustering analysis, two subgenus and seven groups were recognized. The classification of the two subgenus was different from the opinion of subgenus Daiswa and subgenus Paris by Li Heng. The classification of sect. Dunnianae, sect. Axiparis and sect. Paris was correspondence with the classification of Li Heng. But sect. Fargesianae, sect. Marmoratae and sect. Thibeticae which were established based on the especially characters by Li Heng were put into cluster 2, cluster 3 and cluster 4.
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http://dx.doi.org/10.4268/cjcmm20101202DOI Listing
June 2010

[Chemical constituents from herbs of Paris verticillata].

Zhongguo Zhong Yao Za Zhi 2009 Jul;34(14):1812-5

School of Pharmaceutical Science and Technolgy, Tianjin University, Tianjin 300072, China.

Objective: To study the chemical constituents in herbs of Paris verticillata.

Method: The compounds were isolated by column chromatography with silica gel and purified by Sephadex LH-20 and RP-HPLC. The structures were identified by means of NMR analysis.

Result: Nine compounds were isolated from the EtOAc extract and the n-BuOH extract of P. verticillata. Their structures were identified as beta-sitosterol (1), stigmasterol (2), daucosterol (3), beta-ecdysterone (4), 4-hydroxymethyl-gamma-butyrolactone (5), diosgenin-3-O-alpha-L-arabinofuranosyl (1-->4)-[alpha-L-rhamnopyranosyl (1-->2)]-beta-D-glycopyranoside (6), pennogenin-3-O-alpha-L-arabinofuranosyl(1-->4)-beta-D-glycopyranoside (7), pennogenin-3-O-alpha-L-arabinofuranosyl (1-->4)-[alpha-L-rhamnopy-ranosyl (1-->2)]-beta-D-glycopyranoside (8), and pennogenin-3-O-alpha-L-rhamno-pyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1--4)-[alpha-L-rhamnopyranosyl (1-->2)]-beta-D-glycopyranoside (9).

Conclusion: Compounds 1-9 are isolated from P. verticillata for the first time, and compounds 3, 5 are isolated from the genus Paris for the first time. The compounds 6-9 showed certain inhibition activeness of LA-795 cells, especially, the effects of compounds 6, 8 and 9 were more significant.
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July 2009

Characterization of steroidal saponins in saponin extract from Paris polyphylla by liquid chromatography tandem multi-stage mass spectrometry.

Anal Bioanal Chem 2009 Sep 6;395(2):495-505. Epub 2009 Aug 6.

School of Pharmaceutical Science and Technology, Tianjin University, Weijin Road, Tianjin, 300072, China.

Rhizoma Paridis saponins are bioactive steroidal saponins derived from Paris polyphylla. Optimization of the ionization process was performed with electrospray ionization tandem mass spectrometry in both positive and negative-ion modes. Negative-ion ESI was adopted for generation of the precursor deprotonated molecules to achieve the best ionization sensitivity for the analytes. Positive ionization was used to choose the most abundant fragment ion. Furthermore, according to the characteristic fragmentation behavior of known steroidal saponins isolated from this plant (polyphyllin D, formosanin C, gracillin, Paris H, Paris VII, and dioscin), 23 constituents were structurally characterized on the basis of their retention time and ESI analyses, including four pairs of naturally occurring isomers. Five of these 23 constituents were new compounds. The analytical method of LC-MS(n) in positive and negative-ion modes has been developed for the direct structural elucidation of steroid saponins of this kind in plant extracts.
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http://dx.doi.org/10.1007/s00216-009-2987-2DOI Listing
September 2009