Publications by authors named "Xianjun Min"

5 Publications

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Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3.

Cancer Manag Res 2021 19;13:1767-1776. Epub 2021 Feb 19.

Department of Thoracic Surgery, Peking University People's Hospital, Beijing, 100044, People's Republic of China.

Objective: This study set out to investigate the effect of on lung cancer progression through targeted regulation of .

Methods: RT-PCR was employed to detect the expression levels in lung cancer cells and its targeted gene mRNA determined by biological information prediction. MTT, invasion and apoptosis rate tests were employed to detect the proliferation, invasion and apoptosis rate of lung cancer cells over-expressing or those with low expression of and the expression of related proteins.

Results: RT-qPCR results manifested that the level was down-regulated in lung cancer tissues and cells, and the expression increased. The and expression levels were negatively correlated. was correlated with tumor differentiation degree, TNM stage and lymph node metastasis of lung cancer patients. Cell tests confirmed that played a tumor-inhibiting function, including inhibiting proliferation and invasion of lung cancer cells and promoting apoptosis. Bioinformatics prediction and subsequent experiments proved that was the direct target of . Moreover, after the expression in lung cancer cells was knocked down, proliferation and invasion of those cells were inhibited dramatically, the apoptosis rate increased markedly, and the expression levels of pro-apoptosis-related proteins and were up-regulated, and the anti-apoptosis-related protein expression was down-regulated.

Conclusion: can inhibit the proliferation and invasion of lung cancer cells and promote their apoptosis by targeting . It can be used as a new potential target for lung cancer treatment.
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http://dx.doi.org/10.2147/CMAR.S251679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903947PMC
February 2021

The safety and feasibility of intraoperative near-infrared fluorescence imaging with indocyanine green in thoracoscopic sympathectomy for primary palmar hyperhidrosis.

Thorac Cancer 2020 04 15;11(4):943-949. Epub 2020 Feb 15.

Department of Thoracic Surgery, Beijing Haidian Hospital (Haidian Section of Peking University Third Hospital), Beijing, China.

Background: We investigated the safety and feasibility of intraoperative near-infrared (NIR) imaging using indocyanine green (ICG) during sympathectomy in the management of primary palmar hyperhidrosis (PPH).

Methods: We performed a retrospective review of 142 patients (ICG group) who underwent endoscopic thoracic sympathectomy (ETS) between February 2018 and April 2019. All patients received a 5 mg/kg infusion of ICG 24 hours preoperatively. The vital signs before and after ICG injection and adverse reactions were recorded. Meanwhile, 498 patients (Non-ICG group) who underwent ETS by normal thoracoscopy during August 2017 to April 2019 were also reviewed to compare the abnormal white blood cell (WBC) counts, alanine transaminase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and creatinine (Cr) levels before and after operation between two groups.

Results: For ICG group, the vital signs including body temperature, heart rate and blood pressure before and after ICG injection were stable. There was no significant difference in the abnormal WBC counts, ALT, AST, BUN, and Cr levels before and after operation between two groups. Only one patient had mild adverse reaction (0.7%) after ICG injection. The visibility rate of all sympathetic ganglions was 96.7% (1369/1415). The visibility rate from T1 to T5 was 98.23% (278/283), 98.23% (278/283), 97.17% (275/283), 95.76% (271/283), and 94.35% (267/283), respectively. There was no significant difference in the visibility rate with regard to age, gender, height, weight, body mass index, and PPH grade.

Conclusions: NIR fluorescence imaging with ICG for identifying sympathetic ganglions is relatively safe and feasible.

Key Points: • Significant findings of the study. NIR fluorescence imaging with ICG for identifying sympathetic ganglions is relatively safe and feasible. • What this study adds. This technology may take the place of the rib-oriented method as standard practice for the precise localization of sympathetic ganglions, and may improve the effect of sympathectomies.
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http://dx.doi.org/10.1111/1759-7714.13345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113049PMC
April 2020

Mechanical pleurodesis does not reduce recurrence of spontaneous pneumothorax: a randomized trial.

Ann Thorac Surg 2014 Nov 16;98(5):1790-6; discussion 1796. Epub 2014 Sep 16.

Department of Thoracic Surgery, Beijing Haidian Hospital (Haidian Section of Peking University Third Hospital), Beijing, P.R. China; Department of Thoracic Surgery, Peking University People's Hospital, Beijing, P.R. China.

Background: Mechanical pleurodesis is widely used to treat primary spontaneous pneumothorax to decrease postoperative recurrence after thoracoscopic bullectomy, but it is unclear whether it actually reduces primary spontaneous pneumothorax recurrence. We aimed to investigate the effectiveness of mechanical pleurodesis after thoracoscopic treatment of primary spontaneous pneumothorax.

Methods: In our parallel-group, prospective, randomized, controlled trail at 2 hospitals in China, 289 patients were enrolled from January 2010 to January 2013. Patients were randomly assigned (1:1) to receive thoracoscopic wedge resection only (WR group) or thoracoscopic wedge resection and mechanical pleurodesis (WR+MP group). This trial is registered with ClinicalTrial.gov (NCT01463553).

Results: Intraoperative bleeding and postoperative pleural drainage were significantly lower in the thoracoscopic WR only group. Postoperative recurrence rate did not significantly differ between groups (log-rank test p=0.791; Breslow test p=0.722). In the thoracoscopic WR only group, no recurrences were found when bullae were isolated or limited; recurrence was 7.5% with the presence of multiple bullae. Younger patients had an increased risk of recurrence (relative risk 3.015; 95% confidence interval 1.092 to 8.324).

Conclusions: Thoracoscopic mechanical pleurodesis did not significantly decrease primary spontaneous pneumothorax recurrence compared with simple wedge resection, but intraoperative bleeding and postoperative pleural drainage rates were higher. Younger age increases the risk of recurrence.
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http://dx.doi.org/10.1016/j.athoracsur.2014.06.034DOI Listing
November 2014

A primary pulmonary glomus tumor complicated with hyperpyrexia and anemia.

Ann Thorac Surg 2013 Feb;95(2):e29-31

Department of Thoracic Surgery, Peking University People's Hospital, Beijing, PR China.

Pulmonary glomus tumors are extremely rare, with only 19 cases having been reported worldwide. The glomus body is considered to be related to the regulation of body temperature, but the reported cases were not associated with hyperpyrexia. Here, we describe a 28-year-old man with hyperpyrexia and anemia complicated with a coin lesion of the right lung. After resection of the upper lobe of the right lung by video-assisted thoracoscopic surgery, all of the patient's symptoms disappeared. The pathologic analysis reported a rare pulmonary glomus tumor. The disease had not recurred by 1 year after operation.
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http://dx.doi.org/10.1016/j.athoracsur.2012.08.117DOI Listing
February 2013

Activation of volume-sensitive Cl(-) channel is involved in carboplatin-induced apoptosis in human lung adenocarcinoma cells.

Cancer Biol Ther 2010 Jun 1;9(11):885-91. Epub 2010 Jun 1.

Department of Thoracic Surgery, Beijing Chao-Yang Hospital, Beijing, China.

The purpose of the present study is to observe the role of volume-sensitive Cl(-) channels in carboplatin-induced apoptosis in the human lung adenocarcinoma cell line A549 cells. Using patch clamp and apoptosis assays, we found that A549 cells underwent the process of apoptotic volume decrease (AVD) and apoptosis when treated with carboplatin or staurosporine (STS). This AVD and apoptosis process were blocked by chloride channel blockers, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) and 5-nitro-2-(3-phenyl propylamino)-benzoate (NPP B). Both carboplatin and STS treatment activated a Cl(-) current, which shows similar properties to hypotonicity-induced volume-sensitive Cl(-) current in A549 cells. In addition, carboplatin pretreatment augmented the magnitude of the hypoosmotic-induced volume-sensitive Cl(-) current. These results suggest that volume-sensitive Cl(-) channels may be responsible for the carboplatin-induced apoptosis in A549 cells by inducing the AVD process.
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http://dx.doi.org/10.4161/cbt.9.11.11666DOI Listing
June 2010