Publications by authors named "Xiangyu Zhu"

71 Publications

Mitochondrial genome variations are associated with amyotrophic lateral sclerosis in patients from mainland China.

J Neurol 2021 Jun 15. Epub 2021 Jun 15.

Department of Neurology, Xiangya Hospital, Central South University, 87 Xiangya Rd, Changsha, Hunan, People's Republic of China.

Background: Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder. Mitochondrial dysfunction is involved in the complex pathophysiology of ALS; however, the role of mitochondrial DNA (mtDNA) variants in ALS is poorly understood. We aimed to elucidate the role of mtDNA variants in the pathogenesis of ALS.

Methods: The mitochondrial haplogroups of 585 ALS patients and 371 healthy controls were determined; 38 ALS patients and 42 controls underwent long-range polymerase chain reaction combined with next-generation sequencing technology to analyze whole mitochondrial genome variants.

Results: A higher percentage of variants accumulated in ALS patients than in controls. Analysis of coding region variations that were further stratified by mtDNA genes revealed that nonsynonymous variants were more vulnerable in ALS patients than in controls, particularly in the ND4L, ND5, and ATP8 genes. Moreover, pathogenic nonsynonymous variants tended to over-represent in ALS patients. Unsurprisingly, nonsynonymous variants were not related to the phenotype. Haplogroup analysis did not found evidence of association between haplogroups with the risk of ALS, however, patients belonging to haplogroup Y and M7c were prone to develop later onset of ALS.

Conclusions: This is the first study to profile mtDNA variants in ALS patients from mainland China. Our results suggest that an increase in the number of nonsynonymous variants is linked to the pathogenesis of ALS. Moreover, haplogroup Y and M7c may modulate the clinical expression of ALS. Our findings provide independent, albeit limited, evidence for the role of mtDNA in the pathogenesis of ALS.
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http://dx.doi.org/10.1007/s00415-021-10659-7DOI Listing
June 2021

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella.

Pest Manag Sci 2021 May 18. Epub 2021 May 18.

State Key Laboratory of Ecological Pest Control for Fujian-Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Backgroud: Insect biogenic amines play important roles in mediating behavioral and physiological processes. They exert their effects by binding to biogenic amine receptors (BARs), which are specific receptor proteins in the G-protein-coupled receptor superfamily. BAR genes have been cloned and characterized from multiple model insects, including Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Apis mellifera and Tribolium castaneum. However, relatively little work has addressed the molecular properties, expression profiles, and pharmacological characterization of BARs from other insects, including important pests.

Results: In this study, we cloned 17 genes encoding putative biogenic amine receptor proteins from Plutella xylostella, a global pest of Brassica crops. These PxBAR genes were five octopamine receptors (PxOA1, PxOA2B1, PxOA2B2, PxOA2B3, and PxOA3), three tyramine receptors (PxTAR1A, PxTAR1B, and PxTAR2), four dopamine receptors (PxDOP1, PxDOP2, PxDOP3, and PxDopEcR), and five serotonin receptors (Px5-HT , Px5-HT , Px5-HT , Px5-HT , and Px5-HT ). All PxBARs showed considerable sequence identity with orthologous BARs, and phylogenetic analysis clustered the receptors within their respective groups while preserving organismal evolutionary relationships. We investigated their molecular properties and expression profiles, and pharmacologically characterized the dopamine receptor, PxDOP2.

Conclusions: Our study provides important information and resources on biogenic amine receptors from P. xylostella, which suggests potential target sites for controlling this pest species.
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http://dx.doi.org/10.1002/ps.6481DOI Listing
May 2021

Factors associated with prior acute pancreatitis episodes.

Authors:
Xiangyu Zhu

Dig Liver Dis 2021 May 6. Epub 2021 May 6.

Department of Hepatobiliary Surgery, YiWu Central Hospital, Zhejiang 322000, China. Electronic address:

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http://dx.doi.org/10.1016/j.dld.2021.04.011DOI Listing
May 2021

A commentary on "comparison on the efficacy and prognosis of different strategies for intrahepatic recurrent hepatocellular carcinoma: A systematic review and Bayesian network meta-analysis" (Int J Surg 2020; 83:196-204).

Int J Surg 2021 06 2;90:105963. Epub 2021 May 2.

Department of Infectious Diseases, YiWu Central Hospital, Zhejiang, 322000, China. Electronic address:

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http://dx.doi.org/10.1016/j.ijsu.2021.105963DOI Listing
June 2021

Placenta-derived IL-32β activates neutrophils to promote preeclampsia development.

Cell Mol Immunol 2021 04 11;18(4):979-991. Epub 2021 Mar 11.

Department of Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

Immune activation at the maternal-fetal interface is a main pathogenic factor of preeclampsia (PE). Neutrophils (PMNs) are activated in PE patients, but the mechanism and consequences of PMN activation need to be further explored. Here, we demonstrated that interleukin-32 (IL-32) expression was significantly upregulated in syncytiotrophoblasts (STBs) and that IL-32β was the major isoform with increased expression in the placenta of severe PE (sPE) patients. Furthermore, the level of IL-32 expression in the placenta was correlated with its level in the serum of sPE patients, indicating that IL-32 in the serum is derived mainly from the placenta. Then, in vitro experiments showed that IL-32β could highly activate PMNs and that these IL-32β-activated PMNs were better able to adhere to endothelial cells (HUVECs) and enhance the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular cell adhesion molecule-1 (ICAM-1) in HUVECs, which could be reversed by preincubation with the NADPH oxidase inhibitor VAS 2870. In addition, we showed that IL-32β mainly activated PMNs by binding to proteinase 3. Finally, IL-32β administration induced a PE-like phenotype in a pregnant mouse model. This study provides evidence of the involvement of IL-32β in the pathogenesis of PE.
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http://dx.doi.org/10.1038/s41423-021-00636-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115232PMC
April 2021

Smell disorders in COVID-19 patients: role of olfactory training: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Feb;100(8):e24862

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine.

Background: As the coronavirus disease 2019 (COVID-19) spread around the world, a surge of evidence suggests that smell disorders are common symptoms in COVID-19 infection. This dysfunction may cause loss of appetite, malnutrition, poisoning, and depression. Obviously, the impairment has a strong impact on the quality of life. Therefore, there is an urgent need to identify effective treatments. Various therapies have been studied to treat smell disorders after infection, and olfactory training (OT) is considered a promising treatment option. Assessing the effectiveness and safety of olfactory training for COVID-19 patients with smell disorders is the main purpose of this systematic review protocol.

Methods: PubMed, EMBASE, MEDLINE, the Cochrane Library, Chinese National Knowledge Infrastructure, Chinese Biomedical Literature Database, Wanfang Database, ClinicalTrials.gov trials registry, and Chinese Clinical Trial Registry will be searched from January 2019 to January 2021. A combination of subject words and free text words will be applied in the searches. The language is limited to Chinese and English. The complete process will include study selection, data extraction, risk of bias assessment, and meta-analyses. Endnote X9.3 will be used to manage data screening. The statistical analysis will be completed by Review Manager V.5.3 (Cochrane Collaboration) or Stata V.16.0 software.

Results: This proposed study will assess the effectiveness and safety of OT for COVID-19 patients with smell disorders.

Conclusion: The conclusion of this study will provide evidence to prove the effectiveness and safety of olfactory training for COVID-19 patients with smell disorders.

Ethics And Dissemination: This protocol will not evaluate individual patient information or infringe patient rights and therefore does not require ethical approval.

Registration: PEROSPERO CRD42020218009.
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http://dx.doi.org/10.1097/MD.0000000000024862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909207PMC
February 2021

Tai Chi for the elderly patients with COVID-19 in recovery period: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jan;100(3):e24111

School of Acupuncture, Moxibustion and Tuina.

Background: The coronavirus disease 2019 (COVID-19) outbreak has caused a great impact in many countries. Older people are more susceptible to the virus than other people. As a good health exercise suitable for the elderly, Tai Chi has a positive impact on heart function, blood pressure, lung function, immunity, etc. It can enhance cardiopulmonary function, increase the elasticity of blood vessels, and improve the body's self-regulation function. For the elder patients with COVID-19, Tai Chi has outstanding merits.

Methods: We will search PubMed, EMBASE, MEDLINE, the Cochrane Library, Chinese National Knowledge Infrastructure, Chinese Biomedical Literature Database, Chinese Science and Technology Periodical Database, Wanfang Database, Clinical Trials and Chinese Clinical Trial Registry. The complete process will include study selection, data extraction, risk of bias assessment and meta-analyses. Endnote X9.3 will be used to manage data screening. The statistical analysis will be completed by Stata/SE 15.1 software.

Results: This proposed study will evaluate the effectiveness and safety of Tai Chi for the improvement of psychological pressure, cardiopulmonary function, and immunity in elderly COVID-19 patients during the recovery period.

Conclusion: The conclusion of this study will provide evidence to prove the safety and effectiveness of Tai Chi on elderly COVID-19 patients during the recovery period.

Ethics And Dissemination: This protocol will not evaluate individual patient information or infringe patient rights and therefore does not require ethical approval.

Registration: PEROSPERO CRD42020220128.
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http://dx.doi.org/10.1097/MD.0000000000024111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837887PMC
January 2021

Botulinum toxin A improves psychological distress in patients with hemifacial spasm.

Acta Neurol Belg 2021 Jan 29. Epub 2021 Jan 29.

Department of Neurology, Huai'an First People's Hospital, The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, No. 1 Huanghe West Road, Huai'an, 223000, Jiangsu, China.

Objective: This study aimed at assessing mental health in patients with hemifacial spasm (HFS) and determined the effect of botulinum toxin type A (BTX-A) on psychological distress in patients with HFS.

Methods: Ninety-five HFS patients and 95 age- and sex-matched healthy controls were enrolled. Symptom checklist-90 (SCL-90) scores were used to measure psychological distress in HFS patients and healthy controls. The mental health status of HFS patients was also evaluated by SCL-90, before and after the injection of BTX-A. Moreover, for those patients with abnormal mental health, efficacy outcomes after treatment with BTX-A were compared with a propensity score-matched historical cohort without BTX-A treatment.

Results: The mean scores for interpersonal sensitivity, phobia, anxiety, depression, and somatization were significantly higher among HFS patients than healthy people (P < 0.05). There was no significant difference between female patients and male patients in HFS group (P > 0.05). There were significant improvements in somatization, interpersonal sensitivity, depression, anxiety, and phobia scores before and after treatment (P < 0.05). At 2 months, more patients experienced an improvement in psychological distress in the BTX-A group (61.29% versus 38.71%; P = 0.03).

Conclusion: Patients with HFS are often accompanied by somatization, interpersonal sensitivity, depression, anxiety, and phobia. Our findings suggest that BTX-A can improve these symptoms. However, further well-designed prospective studies are warranted to validate our findings.
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http://dx.doi.org/10.1007/s13760-021-01601-9DOI Listing
January 2021

Exercise and Circulating Microparticles in Healthy Subjects.

J Cardiovasc Transl Res 2021 Jan 25. Epub 2021 Jan 25.

Dongzhimen Hospital, Key Laboratory of Chinese Internal Medicine of Ministry of Education, Beijing University of Chinese Medicine, Beijing, 100700, People's Republic of China.

This study aimed to explore the relationship between exercise and circulating microparticles (CMPs). PubMed, Web of Science, Embase, and the Cochrane Library databases were searched until August 13, 2020, using the terms "exercise" and "cell-derived microparticles." The Cochrane tool of risk of bias and the Methodological Index for Non-Randomized Studies were used to grade the studies. Twenty-six studies that met criteria were included in this review, including one before-after self-control study, 2 cohort studies, 4 randomized control trials, 5 case-control studies, and 14 descriptive studies. The studies were divided into a single bout and long-term exercise. The types of MPs contained endothelium-derived microparticles (EMPs), leukocyte-derived microparticles (LMPs), platelet-derived microparticles (PMPs), and erythrocyte-derived microparticles (ErMPs). This first systematic review found that the levels of CMPs continued to increase after a single bout of exercise in untrained subjects and were lower in trained subjects. PMPs expressed a transient increase after a single bout of exercise, and the proportion and duration of PMPs increment reduced in long-term exercise. Most studies showed a decline in LMPs in trained subjects after a single bout and long-term exercise, and variable changes were found in EMPs and ErMPs after exercise. A single bout of exercise drives the vessels exposed to high shear stress that promotes the formation of CMPs. However, the decline in CMPs in trained subjects may be attributed to the fact that they have a better ability to adapt to changes in hemodynamics and cellular function during exercise.
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http://dx.doi.org/10.1007/s12265-021-10100-4DOI Listing
January 2021

Aminotransferases disorders associated with venous thromboembolic events in patients infected with COVID-19.

Ann Hepatol 2021 Jul-Aug;23:100306. Epub 2021 Jan 9.

Department of Infectious Diseases, YiWu Central Hospital, Zhejiang 322000, China. Electronic address:

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http://dx.doi.org/10.1016/j.aohep.2021.100306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796653PMC
July 2021

IL-8 exacerbates alcohol-induced fatty liver disease via the Akt/HIF-1α pathway in human IL-8-expressing mice.

Cytokine 2021 02 22;138:155402. Epub 2020 Dec 22.

Key Laboratory of Anti-inflammatory and Immune Medicines, Ministry of Education, School of Pharmacy, Anhui Medical University, 230032 Hefei, China; The Key Laboratory of Major Autoimmune Diseases, Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, 230032 Hefei, China; Institute for Liver Diseases of Anhui Medical University, 230032 Hefei, China. Electronic address:

Alcoholic fatty liver disease (AFLD) is a disease that causes liver damage due to chronic heavy drinking. AFLD is related to lipid accumulation in liver cells caused by alcohol intake. Interleukin-8 (IL-8) is an inflammatory cytokine associated with chemotaxis (deletion in mice) that has robust effects on the occurrence and development of disease by activating related signal transduction pathways to promote inflammation and cell proliferation. There is significantly increased IL-8 expression in liver disease, which may be related to the pathogenesis of AFLD. In this study, we used hydrodynamic injection to deliver the liver-specific expression vector pLIVE-hIL-8 into mice. We found that hIL-8 can exacerbate alcohol-induced fatty liver disease via the Akt/HIF-1α pathway. Exacerbated liver lipid degeneration in mice, which is characterized by excessive accumulation of triglycerides, and liver damage markers were significantly increased. Moreover, hIL-8 could increase the alcohol-induced release of ROS in fatty liver caused by alcohol and exacerbate fatty liver disease. The expression of liver lipid metabolism-related gene sterol regulatory element-binding protein-1c (SREBP-1c) was increased. Furthermore, the expression of peroxisome proliferator-activated receptor alpha (PPARα), which is related to liver fatty acid oxidation, was decreased. The findings obtained in this study of hIL-8 will help identify a potential target for the clinical treatment of AFLD.
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http://dx.doi.org/10.1016/j.cyto.2020.155402DOI Listing
February 2021

Acupuncture for COVID-19 patient after ventilator weaning: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Dec;99(50):e23602

School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine.

Background: COVID-19 has spread globally since its outbreak in late 2019. It mainly attacks people's respiratory system. Many patients with severe COVID-19 require a ventilator to support breathing, and their lung function is often impaired to varying degrees after ventilator weaning. Acupuncture has been reported to improve respiratory function, but there is no evidence that it can improve respiratory function in ventilator users with COVID-19 after they are removed from the machine. The protocol of the systematic review and meta-analysis will clarify safety and effectiveness of acupuncture on respiratory rehabilitation after weaning from the ventilator during the treatment of COVID-19.

Methods: We will search PubMed, EMBASE, MEDLINE, the Cochrane Library, Chinese National Knowledge Infrastructure, Chinese Biomedical Literature Database, Chinese Science and Technology Periodical Database, Wanfang Database, Clinical Trials and Chinese Clinical Trial Registry. Relevant English language and Chinese language literature will be included. A combination of subject words and free text words will be applied in the searches. The complete process will include study selection, data extraction, risk of bias assessment, and meta-analyses. We will use subgroup analysis and sensitivity analysis to explore the sources of heterogeneity if there is heterogeneity. We will use funnel charts to assess the risk of bias. Endnote X9.3 will be used to manage data screening. The statistical analysis will be completed by RevMan5.2 or Stata/SE 15.1 software.

Results: This study will assess safety and effectiveness of acupuncture for rehabilitation on respiratory function after weaning from the ventilator during the treatment of COVID-19.

Conclusions: The conclusion of this study will give evidence to prove safety and effectiveness of acupuncture for rehabilitation on respiratory after weaning from the ventilator during the treatment of COVID-19.

Registration: PROSPERO CRD42020206889.
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http://dx.doi.org/10.1097/MD.0000000000023602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738103PMC
December 2020

The effect of Tai Chi on the quality of life in the elderly patients recovering from coronavirus disease 2019: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Dec;99(49):e23509

School of Acupuncture and Moxibustion and Tuina.

Background: coronavirus disease 2019 (COVID-19) is spreading fast starting late 2019. As their cardiopulmonary and immune functions gradually decline, elderly people are prone to COVID-19. Tai Chi has a positive impact on heart function, blood pressure, lung function, blood circulation, and so on, and it's suitable for the elderly. Quality of life (QoL)can reflect of individuals' physical and mental health, it can also reflects their ability to participate in society. This systematic review and meta-analysis will summarize the current evidence that Tai Chi improve the QoL in the elderly patients recovering from COVID-19.

Methods: We will search PubMed, EMBASE, MEDLINE, the Cochrane Library, Chinese National Knowledge Infrastructure, Chinese Biomedical Literature Database, Chinese Science and Technology Periodical Database, Wanfang Database, Clinical Trials and Chinese Clinical Trial Registry. The complete process will include study selection, data extraction, risk of bias assessment and meta-analyses. Endnote X9.3 will be used to manage data screening. The statistical analysis will be completed by Stata/SE 15.1 software.

Results: This proposed study will evaluate the effectiveness and safety of Tai Chi for the improvement of QoL in elderly COVID-19 patients during the recovery period.

Conclusion: The conclusion of this study will provide evidence to prove the safety and effectiveness of Tai Chi on elderly COVID-19 patients during the recovery period.

Ethics And Dissemination: This protocol will not evaluate individual patient information or infringe patient rights and therefore does not require ethical approval.

Registration: PEROSPERO CRD42020206875.
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http://dx.doi.org/10.1097/MD.0000000000023509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717820PMC
December 2020

Ethanol abolishes vigilance-dependent astroglia network activation in mice by inhibiting norepinephrine release.

Nat Commun 2020 12 2;11(1):6157. Epub 2020 Dec 2.

Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.

Norepinephrine adjusts sensory processing in cortical networks and gates plasticity enabling adaptive behavior. The actions of norepinephrine are profoundly altered by recreational drugs like ethanol, but the consequences of these changes on distinct targets such as astrocytes, which exhibit norepinephrine-dependent Ca elevations during vigilance, are not well understood. Using in vivo two-photon imaging, we show that locomotion-induced Ca elevations in mouse astroglia are profoundly inhibited by ethanol, an effect that can be reversed by enhancing norepinephrine release. Vigilance-dependent astroglial activation is abolished by deletion of α-adrenergic receptor from astroglia, indicating that norepinephrine acts directly on these ubiquitous glial cells. Ethanol reduces vigilance-dependent Ca transients in noradrenergic terminals, but has little effect on astroglial responsiveness to norepinephrine, suggesting that ethanol suppresses their activation by inhibiting norepinephrine release. Since abolition of astroglia Ca activation does not affect motor coordination, global suppression of astroglial networks may contribute to the cognitive effects of alcohol intoxication.
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http://dx.doi.org/10.1038/s41467-020-19475-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710743PMC
December 2020

Zinc oxide nanoparticles effectively regulate autophagic cell death by activating autophagosome formation and interfering with their maturation.

Part Fibre Toxicol 2020 09 18;17(1):46. Epub 2020 Sep 18.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Beibei, Chongqing, 400715, People's Republic of China.

Background: With the development of zinc oxide nanoparticles (ZnO NPs) in the field of nanotechnology, their toxicological effects are attracting increasing attention, and the mechanisms for ZnO NPs neurotoxicity remain obscure. In an attempt to address concerns regarding neurotoxicity of ZnO NPs, we explored the relationship between free zinc ions, reactive oxygen species (ROS) and neurotoxic mechanisms in ZnO NPs-exposed PC12 cells.

Result: This study demonstrated the requirement of free zinc ions shed by ZnO NPs to over generation of intracellular ROS. Next, we identified autophagic cell death was the major mode of cell death induced by ZnO NPs, and autophagosome accumulation resulted from not only induction of autophagy, but also blockade of autophagy flux. We concluded that autophagic cell death, resulting from zinc ions-ROS-c-Jun N-terminal kinase (JNK)-autophagy positive feedback loop and blockade of autophagosomal-lysosomal fusion, played a major role in the neurotoxicity of ZnO NPs.

Conclusion: Our study contributes to a better understanding of the neurotoxicity of ZnO NPs and might be useful for designing and developing new biosafety nanoparticles in the future.
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http://dx.doi.org/10.1186/s12989-020-00379-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501661PMC
September 2020

Importers Drive Leaf-to-Leaf Jasmonic Acid Transmission in Wound-Induced Systemic Immunity.

Mol Plant 2020 10 2;13(10):1485-1498. Epub 2020 Sep 2.

Department of Ecology, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, P. R. China. Electronic address:

The transmission of mobile wound signals along the phloem pathway is essential to the activation of wound-induced systemic response/resistance, which requires an upsurge of jasmonic acid (JA) in the distal undamaged leaves. Among these mobile signals, the electrical signal mediated by the glutamate-dependent activation of several clade three GLUTAMATE RECEPTOR-LIKE (GLR3) proteins is involved in the stimulation of JA production in distal leaves. However, whether JA acts as a mobile wound signal and, if so, how it is transmitted and interacts with the electrical signal remain unclear. Here, we show that JA was translocated from the local to distal leaves in Arabidopsis, and this process was predominantly regulated by two phloem-expressed and plasma membrane-localized jasmonate transporters, AtJAT3 and AtJAT4. In addition to the cooperation between AtJAT3/4 and GLR3.3 in the regulation of long-distance JA translocation, our findings indicate that importer-mediated cell-cell JA transport is important for driving the loading and translocation of JA in the phloem pathway in a self-propagating manner.
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http://dx.doi.org/10.1016/j.molp.2020.08.017DOI Listing
October 2020

Can polymorphisms of AMH/AMHR2 affect ovarian stimulation outcomes? A systematic review and meta-analysis.

J Ovarian Res 2020 Sep 4;13(1):103. Epub 2020 Sep 4.

Center for Reproductive medicine, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, China.

Background: Previous studies have investigated the effects of anti-Müllerian hormone (AMH) and AMH type II receptor (AMHR2) polymorphisms on ovarian stimulation outcomes, but the results were inconsistent.

Methods: We searched PubMed, Web of Science, Embase, and Cochrane Central Register of Controlled Trials databases for the literature used in this meta-analysis. The meta-analysis was performed with a random effects model with RevMan 5.3.5. Results were expressed as the relative risk (RR) for discrete data and the mean difference (MD) for continuous outcomes with a 95% confidence interval (CI).

Results: Seven studies with 2078 participants were included. More metaphase II (MII) oocytes were retrieved in the T allele carrier of AMH (rs10407022) in the dominant model (MD: 1.20, 95% CI: 0.76 to 1.65, I = 0%, P < 0.00001), homozygote model (MD: 1.68, 95% CI: 0.35 to 3.01, I = 70%, P = 0.01) and heterogeneity model (MD: 1.20, 95% CI: 0.74 to 1.66, I = 0%, P < 0.00001). Oocytes retrieved from the Asian region in the TT carrier were significantly lesser than those in the GG/GT carrier in AMH (rs10407022) (MD: -1.41, 95% CI: - 1.75 to - 1.07, I = 0%). Differences in the stimulation duration, gonadotropin (Gn) dosage, and pregnancy rate were insignificant.

Conclusions: Our analysis indicated that the polymorphisms of AMH/AMHR2 could influence the ovarian stimulation outcomes. Prospective studies with a larger sample size and more rigorous design are needed in the future to further confirm these findings.
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http://dx.doi.org/10.1186/s13048-020-00699-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487641PMC
September 2020

Compromised Autophagic Effect of Polystyrene Nanoplastics Mediated by Protein Corona Was Recovered after Lysosomal Degradation of Corona.

Environ Sci Technol 2020 09 25;54(18):11485-11493. Epub 2020 Aug 25.

Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Beibei, Chongqing 400715, People's Republic of China.

The adverse biological and ecological consequences of plastic debris have become a serious problem worldwide. Evidences have uncovered the accumulation of nanoplastics (NPs) in organisms. In a complex biological environment, proteins are prone to adsorbed onto the NPs' surface and form a protein corona layer, which mediates the interaction of NPs with cells. Here, we discovered the interaction of polystyrene (PS) NPs with protein fetal bovine serum (FBS) and altered cytotoxic effects. Mechanistically, prefabricated FBS protein corona mediated the relief of autophagic flux blockage, autophagosomes accumulation, and lysosomal damage in RAW264.7 cells caused by PS NPs. Using an individual fluorescent protein bovine serum albumin (BSA) as a corona surrogate, we demonstrated that coronal BSA remains, at least partially, on the surface of PS NPs during the initial stage of internalization and protects cell membrane from PS NPs-induced damage. However, along with the degradation of corona in lysosomes, reappearance of cytotoxicity was observed. Herein, we provided a proof of principle of the manipulation of corona on NPs' toxicity and we expect the result will promote the further safety assessment of NPs.
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http://dx.doi.org/10.1021/acs.est.0c04097DOI Listing
September 2020

Molecular characteristics of the capsid protein VP2 gene of canine parvovirus type 2 amplified from raccoon dogs in Hebei province, China.

Arch Virol 2020 Nov 7;165(11):2453-2459. Epub 2020 Aug 7.

Key Laboratory of Special Animal Epidemic Disease of Ministry of Agriculture and Rural Affairs, Institute of Special Animals and Plants Sciences, Chinese Academy of Agricultural Sciences, No. 4899 Juye Street, Changchun, 130112, Jilin, China.

Canine parvovirus type 2 (CPV-2) is currently circulating in domestic and wild animals, but our knowledge about CPV-2 infections in raccoon dogs is limited. In this study, VP2 gene sequences of CPV-2 were amplified from rectal swabs of 14 diarrhetic raccoon dogs (Nyctereutes procyonoides) in Hebei province, China, in 2016 and 2017. Phylogenetic analysis of the VP2 gene sequences revealed that most of these sequences (11 of 14) belonged to the same subclade as raccoon dog strain CPV-2/Raccoon_Dog/China/DP-1/16 isolated from Shandong province in 2016. A comparison of deduced amino acid sequences revealed presence of the substitutions S297A and S27T in 11 of those 14 sequences. I418T was observed in a minority of the sequences (4 of 14). In addition, A300D and T301I, P13S and I219V, and N419K were found in three of the sequences. This study shows that CPV-2 strains with different substitutions in their VP2 amino acid sequences were spreading among raccoon dogs in Hebei during 2016 and 2017 and suggests that further studies are needed to monitor the distribution of these strains in China.
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http://dx.doi.org/10.1007/s00705-020-04714-3DOI Listing
November 2020

Melatonin Alleviates Neuronal Damage After Intracerebral Hemorrhage in Hyperglycemic Rats.

Drug Des Devel Ther 2020 2;14:2573-2584. Epub 2020 Jul 2.

Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.

Background: This study sought to investigate a novel effect of melatonin in reducing brain injury in an in vivo hyperglycemic intracerebral hemorrhage (ICH) model and further explore the mechanisms of protection.

Methods: Hyperglycemia ICH was induced in Sprague-Dawley rats by streptozocin injection followed by autologous blood injection into the striatum. A combined approach including RNA-specific depletion, electron microscopy, magnetic resonance, Western blots, and immunohistological staining was applied to quantify the brain injuries after ICH.

Results: Hyperglycemia resulted in enlarged hematoma volume, deteriorated brain edema, and aggravated neuronal mitochondria damage 3 days after ICH. Post-treatment with melatonin 2 hours after ICH dose-dependently improved neurological behavioral performance lasting out to 14 days after ICH. This improved neurological function was associated with enhanced structural and functional integrity of mitochondria. Mechanistic studies revealed that melatonin alleviated mitochondria damage in neurons via activating the PPARδ/PGC-1α pathway. Promisingly, melatonin treatment delayed until 6 hours after ICH still reduced brain edema and improved neurological functions. Melatonin supplementation reduces neuronal damage after hyperglycemic ICH by alleviating mitochondria damage in a PPARδ/PGC-1α-dependent manner.

Conclusion: Melatonin may represent a therapeutic strategy with a wide therapeutic window to reduce brain damage and improve long-term recovery after ICH.
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http://dx.doi.org/10.2147/DDDT.S257333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7342491PMC
June 2021

Intermittent pneumatic compression combined with rehabilitation training improves motor function deficits in patients with acute cerebral infarction.

Acta Neurol Belg 2020 Jul 30. Epub 2020 Jul 30.

Department of Neurology, The Second People's Hospital of Huai'an, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, 223002, Jiangsu, China.

To investigate the effect of intermittent pneumatic compression (IPC) combined with rehabilitation training on patients with acute cerebral infarction and motor impairment, seventy-four patients with acute cerebral infarction and hemiplegia were randomly and equally divided into two groups, the control group and the IPC treatment group. The patients in the control group received conventional drug therapy and rehabilitation training, and the patients in the treatment group received the IPC treatment in addition to the treatment given in the control group. Motor function, the primary outcome, of the two groups was evaluated by Fugl-Meyer motor function scores. The Barthel index assessment scale was used to evaluate the ability to perform activities of daily living of the two groups, as a secondary outcome. All these indicators were collected and compared before treatment and at 7 days, 14 days, and 30 days after treatment. The incidence of adverse reactions associated with treatment was also recorded. At 7, 14, and 30 days after treatment, the Fugl-Meyer scores (27.16 ± 7.37, 33.41 ± 7.16 and 38.72 ± 7.65) and Barthel scores (47.16 ± 7.37, 52.41 ± 7.16, and 56.09 ± 8.32) of the treatment group were also significantly higher than those (23.65 ± 3.11, 26.13 ± 3.25, and 28.75 ± 5.92; 44.15 ± 3.11, 46.63 ± 3.25 and 47.75 ± 4.22) of the control group (all P < 0.05). With the extension of follow-up time, both scores were higher. There were no treatment-related adverse events in either of the two groups of patients during or after treatment. In conclusion, the IPC combined with rehabilitation training can effectively improve motor function deficits, the ability to perform activities of daily living, and quality of life for patients.
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http://dx.doi.org/10.1007/s13760-020-01414-2DOI Listing
July 2020

Lack of association between LGMN and Alzheimer's disease in the Southern Han Chinese population.

Eur J Neurosci 2020 10 28;52(8):4009-4017. Epub 2020 Jun 28.

Department of Geriatrics Neurology, Xiangya Hospital, Central South University, Changsha, China.

Recently, functional studies have demonstrated that legumain (LGMN) cleaves both amyloid β-protein precursor and tau, promoting senile plaques and formation of neurofibrillary tangles, which may play a crucial role in the pathogenesis of Alzheimer's disease (AD). However, the genetic role of LGMN in AD has not been clearly elucidated. Here, we used Sanger sequencing to investigate the single independent (single-variant association test) and cumulative (gene-based association test) effects of variants in the LGMN gene as potential susceptibility factors for AD, in a cohort comprising 676 AD cases and 365 elderly controls from the Han population of South China. In single-variant association analysis, none of the common variants in LGMN were statistically significant. In gene-based analysis, the LGMN gene also showed no association with AD. The results of our replication study in the Alzheimer's Disease Neuroimaging Initiative cohort also showed no association between LGMN and AD. These findings suggest that the LGMN gene may not be a critical factor for AD development.
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http://dx.doi.org/10.1111/ejn.14857DOI Listing
October 2020

Which risk factors are associated with stomal recurrence after total laryngectomy for laryngeal cancer? A meta-analysis of the last 30 years.

Braz J Otorhinolaryngol 2020 Jul - Aug;86(4):502-512. Epub 2020 Apr 11.

Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Department of Head and Neck Surgery, Shenyang, People's Republic of China. Electronic address:

Introduction: Stomal recurrence is a troublesome complication after total laryngectomy. Despite a large number of studies having been performed, there is still controversy about which risk factors are most significant for the development of stomal recurrence.

Objective: The objective of the present meta-analysis was to analyze the potential factors leading to stomal recurrence after total laryngectomy.

Methods: PubMed, Web of Science, Cochrane Library, and Ovid databases were systematically searched using multiple search terms. Eighteen studies with 6462 patients were identified. The quality of evidence was assessed by The National Institute for Health and Clinical Excellence.

Results: The results showed that, tumor subsite (supraglottic vs. subglottic, RR=0.292, 95% CI 0.142-0.600, p=0.001; glottic vs. subglottic, RR=0.344, 95% CI 0.175-0.676, p=0.002), T stage (RR=0.461, 95% CI 0.286-0.742, p=0.001), preoperative tracheotomy (RR=1.959, 95% CI 1.500-2.558, p<0.001) were the high-risk factors associated with the development of stomal recurrence.

Conclusion: From the results of our study, tumor subsite, T stage and preoperative tracheotomy were the significant risk factors for stomal recurrence. Methodologically high-quality comparative investigations are needed for further evaluation.
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http://dx.doi.org/10.1016/j.bjorl.2020.03.002DOI Listing
September 2020

CYP4A11 is involved in the development of nonalcoholic fatty liver disease via ROS‑induced lipid peroxidation and inflammation.

Int J Mol Med 2020 Apr 28;45(4):1121-1129. Epub 2020 Jan 28.

Key Laboratory of Major Autoimmune Diseases, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei, Anhui 230032, P.R. China.

Nonalcoholic fatty liver disease (NAFLD) is a fat metabolism disorder that occurs in liver cells. The development of NAFLD is considered to be associated with hepatic oxidative stress. The present study aimed to investigate the role of cytochrome P450 4A11 (CYP4A11) in the pathogenesis of NAFLD. The levels of plasma CYP4A11 and lipid peroxidation products levels exhibited a high correlation, and were increased significantly compared with those from normal subjects. Further in vitro studies demonstrated that the expression levels of CYP4A11 and the content of reactive oxygen species (ROS) were increased in free fatty acid (FFA)‑stimulated HepG2 cells. Clofibrate, a CYP4A11 inducer, aggravated cell damage. Opposite results were observed for the CYP4A11 inhibitor HET0016, which attenuated apoptosis in FFA‑treated cells. Furthermore, CYP4A11 gene overexpression and silencing were used to investigate the effects on inflammatory cytokine secretion. The data demonstrated that CYP4A11 promoted an increase in the mRNA expression of tumor necrosis factor α, interleukin (IL)‑1β and IL‑6 in response to FFA. In addition, western blot analysis highlighted that CYP4A11 caused an upregulation of phosphorylated p65 levels and therefore affected the NF‑κB signaling pathway. The data demonstrated that CYP4A11 may metabolize fatty acids to promote the production of ROS and accelerate the progression of NAFLD.
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http://dx.doi.org/10.3892/ijmm.2020.4479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053872PMC
April 2020

A study on the efficacy of recombinant human endostatin combined with apatinib mesylate in patients with middle and advanced stage non-small cell lung cancer.

J BUON 2019 Nov-Dec;24(6):2267-2272

Department of Thoracic Surgery, Cancer Hospital of China Medical University, Shenyang 110042, China.

Purpose: To explore the clinical efficacy of recombinant human endostatin combined with apatinib mesylate in patients with middle and advanced non-small cell lung cancer (NSCLC).

Methods: A total of 64 patients with middle and advanced NSCLC were randomly divided into the control group (n=32) and observation group (n=32). The patients in control group received paclitaxel monotherapy, while those in the observation group were treated with recombinant human endostatin combined with apatinib mesylate. The short-term efficacy, the lung function and levels of immunoglobulin and T lymphocyte subsets before and after treatment and the adverse drug reactions of patients were compared between the two groups. All patients were followed up for 5 years, and the survival rate in the two groups was observed.

Results: The short-term efficacy and lung function in observation group were better than those in control group (p<0.05). Compared with those in the control group, the levels of immunoglobulin G (IgG), IgA, IgM, cluster of differentiation 3+ (CD3+), CD4+ and CD4+/CD8+ were increased, while the CD8+ level was lowered in the observation group (p<0.05). The rate of adverse drug reactions in the observation group was lower than that in the control group (p<0.05). The 5-year survival rate was significantly higher in the observation group than that in the control group (p<0.05).

Conclusion: Recombinant human endostatin combined with apatinib mesylate achieves a better therapeutic effect in the treatment of middle and advanced NSCLC, with improved immune resistance of patients and less side effects. Therefore, it is worthy of popularization and application in clinical practice.
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June 2020

Precise control of versatile microstructure and properties of graphene aerogel via freezing manipulation.

Nanoscale 2020 Feb;12(8):4882-4894

State Key Laboratory for Advanced Metals and Materials, University of Science and Technology Beijing, Beijing 100083, China.

A deep understanding of the shaping technique is urgently required to precisely tailor the pore structure of a graphene aerogel (GA) in order to fit versatile application backgrounds. In the present study, the microstructure and properties of GA were regulated by freeze-casting using an ice crystal template frozen from -10 °C to -196 °C. The phase field simulation method was applied to probe the microstructural evolution of the graphene-H2O system during freezing. Both the experimental and simulation results suggested that the undercooling degree was fundamental to the nucleation and growth of ice crystals and dominated the derived morphology of GA. The pore size of GA was largely regulated from 240 to 6 μm via decreasing the freezing temperature from -10 °C to -196 °C but with a constant density of 8.3 mg cm-3. Rapid freeze casting endowed GA with a refined pore structure and therefore better thermal, electrical, and compressive properties, whereas the GA frozen slowly had superior absorption properties owing to the continuous and tube-like graphene lamellae. The GA frozen at -196 °C exhibited the highest Young's modulus of 327 kPa with similar densities to those reported in the literature. These findings demonstrate the diverse potential applications of GA with regulated pore morphologies and also contribute to cryogenic-induced phase separation methods.
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http://dx.doi.org/10.1039/c9nr07861dDOI Listing
February 2020

Co-occurrence patterns and assembly processes of microeukaryotic communities in an early-spring diatom bloom.

Sci Total Environ 2020 Apr 22;711:134624. Epub 2019 Nov 22.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo, 315211, China. Electronic address:

The interaction and assembly processes of microeukaryotic community compositions (MECs) are rarely elucidated in environment with strong disturbance such as harmful algal blooms. To fill this gap, we analyzed changes of MECs induced by a diatom bloom using 18S rRNA gene amplicon sequencing. The MECs were mainly dominated by Cercozoa (average relative abundance, 49.2%), Diatom (25.5%) and Dinoflagellata (15.6%). MECs changed significantly (ANOSIM P < 0.01) in four-bloom stages. Environmental factors including pH, DO, nitrate and phosphate, together with bacterial communities could significantly influence the variation of MECs. Co-occurrence network analysis revealed a complex interaction between microeukaryotic and bacterial communities. Most OTUs in modules of the co-occurrence network were specific to one particular bloom stage. Phylogenetic based β-nearest taxon distance analyses revealed that stochastic processes mainly dominated microeukaryotic community assembly in the initial and after-bloom stage. However, microeukaryotic community assembly in middle and late stage of the bloom were driven by deterministic processes. In conclusion, both stochastic and deterministic processes play important roles in distinct bloom stages. These findings may expand current understandings of assembly mechanisms and microbial interactions underlying microeukaryotic dynamics in eutrophic aquatic ecosystems where harmful algal blooms occurred frequently.
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http://dx.doi.org/10.1016/j.scitotenv.2019.134624DOI Listing
April 2020

[Genetic study of a pedigree affected with oculodentodigital dysplasia].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2019 Dec;36(12):1191-1194

Prenatal Diagnosis Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, China.

Objective: To explore the genetic basis of a pedigree affected with oculodentodigital dysplasia.

Methods: Genomic DNA was extracted from peripheral blood or amniotic fluid samples derived from the pedigree. Exon 2 of the GJA1 gene was amplified for sequencing.

Results: Two pedigree members were found to carry heterozygous missense variation of the GJA1 gene, c.221A>C (p.H74P).

Conclusion: The missense c.221A>C variation of the GJA1 gene probably underlies the oculodentodigital dysplasia in this pedigree.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2019.12.010DOI Listing
December 2019

Negative regulation of cadmium tolerance in Arabidopsis by MMDH2.

Plant Mol Biol 2019 Nov 15;101(4-5):507-516. Epub 2019 Oct 15.

Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, 230009, China.

Key Message: MMDH2 gene negatively regulates Cd tolerance by modulating reactive oxygen species (ROS) levels and the ROS-mediated signaling, thus, affecting the expression of PDR8. The molecular mechanism by which plants respond to stress caused by cadmium (Cd), one of the most toxic heavy metals to plants, is not well understood. Here, we show that MMDH2, a gene encoding mitochondrial malate dehydrogenase, is involved in Cd stress tolerance in Arabidopsis. The expression of MMDH2 was repressed by Cd stress. The mmdh2 knockdown mutants showed enhanced Cd tolerance, while the MMDH2-overexpressing lines were sensitive to Cd. Under normal and Cd stress conditions, lower HO levels were detected in mmdh2 mutant plants than in wild-type plants. In contrast, higher HO levels were found in MMDH2-overexpressing lines, and they were negatively correlated with malondialdehyde levels. In addition, the expression of the PDR8, a gene encoding a Cd efflux pump, increased and decreased in the mmdh2 mutant and MMDH2-overexpressing lines, in association with lower and higher Cd concentrations, respectively. These results suggest that the MMDH2 gene negatively regulates Cd tolerance by modulating reactive oxygen species (ROS) levels and the ROS-mediated signaling, thus, affecting the expression of PDR8.
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http://dx.doi.org/10.1007/s11103-019-00923-wDOI Listing
November 2019

Interaction and assembly processes of abundant and rare microbial communities during a diatom bloom process.

Environ Microbiol 2020 05 11;22(5):1707-1719. Epub 2019 Dec 11.

Environmental Monitoring Center of Ningbo, Ningbo, 315010, China.

Diatom blooms can significantly influence the dynamics of microbial communities, yet little is known about the interaction and assembly mechanisms of abundant and rare taxa during bloom process. Here, using 16S rRNA gene amplicon sequencing, we investigated the co-occurrence patterns and assembly processes of abundant and rare microbial communities during an early spring diatom bloom in Xiangshan bay. Our results showed that α-diversity indices in the rare subcommunity (RS) were significantly higher than those in the abundant and common subcommunities. β-Diversity of the RS was the highest among three subcommunities, and the variation of β-diversity in the three subcommunities was mainly induced by species turnover, which was also the highest in the RS. The assembly of microbial communities was mainly driven by the neutral processes, but the roles of neutral processes might differ in each subcommunity. Co-occurrence network analysis revealed that abundant and common operational taxonomic units were more often located in central positions within the network. Most of the modules in the network were specific to a particular bloom stage, owing to the succession of Skeletonema costatum. Overall, these findings expand current understanding of the microbial interaction and assembly mechanisms in marine environment suffering harmful algal bloom disturbance.
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http://dx.doi.org/10.1111/1462-2920.14820DOI Listing
May 2020
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