Publications by authors named "Xiangrong Li"

70 Publications

Myristoylation-mediated phase separation of EZH2 compartmentalizes STAT3 to promote lung cancer growth.

Cancer Lett 2021 Jun 5. Epub 2021 Jun 5.

Institutes of Biological and Medical Sciences, Soochow University Medical School, 199 Ren'ai Road, Suzhou, Jiangsu, 215123, China. Electronic address:

N-myristoylation is a crucial signaling and pathogenic modification process that confers hydrophobicity to cytosolic proteins. Although different large-scale approaches have been applied, a large proportion of myristoylated proteins remain to be identified. EZH2 is overexpressed in lung cancer cells and exerts oncogenic effects via its intrinsic methyltransferase activity. Using a well-established click chemistry approach, we found that EZH2 can be modified by myristoylation at its N-terminal glycine in lung cancer cells. Hydrophobic interaction is one of the main forces driving or stabilizing liquid-liquid phase separation (LLPS), raising the possibility that myristoylation can modulate LLPS by mediating hydrophobic interactions. Indeed, myristoylation facilitates EZH2 to form phase-separated liquid droplets in lung cancer cells and in vitro. Furthermore, we provide evidence that myristoylation-mediated LLPS of EZH2 compartmentalizes its non-canonical substrate, STAT3, and activates STAT3 signaling, ultimately resulting in accelerated lung cancer cell growth. Thus, targeting EZH2 myristoylation may have significant therapeutic efficacy in the treatment of lung cancer. Altogether, these observations not only extend the list of myristoylated proteins, but also indicate that hydrophobic lipidation may serve as a novel incentive to induce or maintain LLPS.
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http://dx.doi.org/10.1016/j.canlet.2021.05.035DOI Listing
June 2021

Exploring the interactions of naringenin and naringin with trypsin and pepsin: Experimental and computational modeling approaches.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Sep 21;258:119859. Epub 2021 Apr 21.

School of Medical Engineering, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. Electronic address:

Naringenin and naringin are two natural compounds with important health benefits, whether as food or drug. It is necessary to study the interactions between naringenin/naringin and digestive proteases, such as trypsin and pepsin. In this study, the bindings of naringenin and naringin to trypsin and pepsin were investigated using multi-spectroscopy analysis and computational modeling approaches. Fluorescence experiments indicate that both naringenin and naringin can quench the intrinsic fluorescence of trypsin/pepsin via static quenching mechanism. Naringin binds trypsin/pepsin in a more firmly way than naringenin. Thermodynamic analysis reveals that the interactions of naringenin/naringin and trypsin/pepsin are synergistically driven by enthalpy and entropy, and the major driving forces are hydrophobic, electrostatic interactions and hydrogen bonding. Synchronous fluorescence spectroscopy, circular dichroism spectroscopy and FT-IR show that naringenin/naringin may induce microenvironmental and conformational changes of trypsin and pepsin. Molecular docking reveals that naringenin binds in the close vicinity of the active site (Ser-195) of trypsin and Asp-32 (the catalytic activity of pepsin) appears in naringin-pepsin system. The direct interactions between naringenin or naringin and catalytic amino acid residues will inhibit the catalytic activity of trypsin and pepsin, respectively. The results of molecular dynamic simulation validate the reliability of the docking results.
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http://dx.doi.org/10.1016/j.saa.2021.119859DOI Listing
September 2021

Bioassay-guided isolation and characterization of antibacterial compound from HX-1 associated with Clam.

3 Biotech 2021 Apr 25;11(4):193. Epub 2021 Mar 25.

Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, 222005 China.

This study aimed to identify a symbiotic fungus strain HX-1 with anti- activity and isolate and identify the active compound. The HX-1 strain was identified as according to the morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The compound was isolated from the fermentation product of HX-1 strain through ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatography, and semi-preparative HPLC techniques using an antibacterial-guided fractionation method. According to its physicochemical properties and spectral characteristics, the compound was identified as trypacidin having the same anti- activity as streptomycin sulfate, with the minimum inhibitory concentration of 31.25 µg/mL.
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http://dx.doi.org/10.1007/s13205-021-02754-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994498PMC
April 2021

Directing photocatalytic pathway to exceedingly high antibacterial activity in water by functionalizing holey ultrathin nanosheets of graphitic carbon nitride.

Water Res 2021 Jun 8;198:117125. Epub 2021 Apr 8.

Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang, 110016, China; University of Illinois at Urbana-Champaign, Urbana, IL 61801 United States. Electronic address:

Metal-free polymeric carbon nitride (CN) photocatalysts offer attractive technological advantages over the conventional transition metal oxides or sulfides -based photocatalysts in water disinfection, but their antimicrobial activities are limited by their rapid charge carrier recombination and low specific surface areas. By controlling photocatalytic pathways, we obtained in amino-rich holey ultrathin g-CN nanosheets (AHUCN) a highly efficient inactivation rate against E-coli, which is the highest among the monolithic g-CN and exceeds the antibacterial performance of the most of the previously reported g-CN-based photocatalysts. Both the experiments and theoretical calculations demonstrated that the high photocatalytic disinfection performance of AHUCN was derived from the synergistic advantages of their unique holey ultrathin structure and the amino - rich surface in controlling the charge separation and transfer, and most importantly in increasing the photo-production of the dominant antibacterial species, HO. From the analysis of the reactive oxygen species and rotating disk electrode (RDE) measurements, it was found that the presence of abundant surface amino groups enabled the switch of the oxygen-reduction pathway from the two-step single-electron indirect reduction on holey ultrathin g-CN nanosheets (HUCN) to the one-step two-electron direct reduction on AHUCN. The switch of the HO production pathway not only facilitated the separation of photogenerated electron-hole pairs but also promoted the generation of reactive oxygen species, greatly enhancing photocatalytic disinfection efficiency.
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http://dx.doi.org/10.1016/j.watres.2021.117125DOI Listing
June 2021

Relationships of organisational justice, psychological capital and professional identity with job burnout among Chinese nurses: A cross-sectional study.

J Clin Nurs 2021 Apr 7. Epub 2021 Apr 7.

Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, China.

Aims And Objectives: The present study was aimed at describing the status of job burnout and exploring the mediating roles of psychological capital and professional identity on the association between organisational justice and job burnout.

Background: With the shortage of nurses all over the world, nurses' job burnout has become the focus of studies in recent years. However, limited published research has examined the mediating roles of psychological capital and professional identity on the association between organisational justice and job burnout within hospital contexts in China.

Design: A cross-sectional design.

Methods: A total of 1,009 nurses were enrolled from a major general hospital located in Qiqihar City in the northeast of China from March-July 2018, and asked to complete questionnaires regarding organisational justice, psychological capital, professional identity and job burnout. The STROBE checklist was adhered to in this study.

Results: It was observed that the overall proportion of nurses with job burnout was 58.8%. All the correlations among organisational justice, psychological capital, professional identity and job burnout were statistically significant, with coefficients ranging between -0.487-0.863. Psychological capital and professional identity had indirect effects equal to -0.072 and -0.142, respectively, on the association between organisational justice and job burnout, taking up 13.7% and 26.9% of the total effect, respectively. The serial indirect effect of psychological capital and professional identity was -0.129, accounting for 24.5% of the total effect.

Conclusions: This study showed the necessity of addressing the status of job burnout among Chinese nurses. Psychological capital and professional identity may mediate the linkage between organisational justice and job burnout independently and accumulatively.

Relevance To Clinical Practice: Nursing managers should strengthen organisational justice and improve the psychological capital and professional identity of nurses through effective strategies to prevent the job burnout of nurses.
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http://dx.doi.org/10.1111/jocn.15797DOI Listing
April 2021

The complete chloroplast genome sequence of Delile.

Mitochondrial DNA B Resour 2021 Mar 19;6(3):1134-1135. Epub 2021 Mar 19.

Key Laboratory of Crops Genetic Improvement of Guangdong, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

Delile is widely used in folkloric medicine for the treatment of various diseases. In this study, the complete chloroplast genome of Delile was reported, which was assembled and annotated base on genome high-throughput sequencing data. This work provided the clues for the taxonomy of the herb and the potential to utilize the chloroplast genome sequence as a new study target. The length of Delile chloroplast genome was 153,133bp, with two single-copy regions, each has the length of 84,245bp and 13,152bp respectively. This region were separated by two inverted repeat regions with 27,868bp in length. It was predicted to consist of 131 genes in total, including 86 protein-coding genes, 37 tRNA genes, 8 rRNA genes with GC content at 37.68%. Phylogenetic analysis by RAxML (Random Axelerated Maximum Likelikhood) showed Delile is closest to in .
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http://dx.doi.org/10.1080/23802359.2021.1902411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995831PMC
March 2021

Establishment and associated factors of health records among young Chinese migrants.

Environ Health Prev Med 2021 Mar 24;26(1):39. Epub 2021 Mar 24.

Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China.

Background: Most Chinese migrants have been faced with obstacles to getting access to local public health services. Young migrants among internal migrants make a major contribution to the economy. However, the establishment of their health records has been ignored. This research was aimed at exploring the status and associated factors of the establishment of health records among young Chinese migrants.

Methods: Data were obtained from the 2017 China Migrants Dynamic Survey (CMDS). Chi-square test and binary logistic regression were performed to investigate the associated factors of the establishment of health records among young Chinese migrants.

Results: Approximately 30.2% of young migrants had their health records established in inflow communities. Urban residence, medical insurance (insured), and lower average monthly household income were attributed to the establishment of more health records. Young migrants who were in northeast China and across provinces and immigrated for working or engaging in trade were less likely to have health records established. Young migrants who participated in social activities and public affairs activities and took type of people in touch as natives in the inflow area showed a higher possibility of establishing health records. Meanwhile, receiving health education and hearing about national basic public health services (BPHSs) were beneficial for establishing the health records of more young migrants.

Conclusion: This study showed that the health records of young migrants had a relatively low establishment rate. Sociodemographic and migration characteristics, health status, public health services, and social integration factors were all related to the health record establishment of young Chinese migrants.
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http://dx.doi.org/10.1186/s12199-021-00961-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989406PMC
March 2021

Relationships of professional identity and psychological reward satisfaction with subjective well-being among Chinese nurses.

J Nurs Manag 2021 Jan 27. Epub 2021 Jan 27.

Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, China.

Aim: This study aimed at exploring the impact of professional identity and psychological reward satisfaction on subjective well-being and clarifying the effect of psychological reward satisfaction on this relationship.

Background: People know little about the effect of psychological reward satisfaction on the relationship between professional identity and subjective well-being.

Methods: A cross-sectional survey was carried out on 1,009 nurses from Qiqihar City, Heilongjiang Province of China. Professional Identity Scale, Psychological Reward Satisfaction Scale and General Well-Being Schedule were used to assess professional identity, psychological reward satisfaction and subjective well-being, respectively. Associations were explored by using structural equation modelling.

Results: The subjective well-being of 436 (43.2%) nurses was at low and moderate levels. After the adjustment of potential confounding factors, professional identity was still associated with subjective well-being (B = 3.035, β = 0.215, p < .001). Professional identity (r = .308) and psychological reward satisfaction (r = .309) were positively correlated with subjective well-being. Psychological reward satisfaction mediated the association between professional identity and subjective well-being (effect = 0.114, p < .001).

Conclusion: This study suggested that the subjective well-being of nurses should be improved by paying special attention to them and taking targeted support measures.

Implications For Nursing Management: Nursing managers can help enhance the professional identity of nurses by organising nursing education activities, and pay more attention to psychological reward satisfaction to improve the subjective well-being of nurses.
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http://dx.doi.org/10.1111/jonm.13276DOI Listing
January 2021

Encephalomyocarditis Virus Abrogates the Interferon Beta Signaling Pathway via Its Structural Protein VP2.

J Virol 2021 02 24;95(6). Epub 2021 Feb 24.

Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, China

Type I interferon (IFN)-mediated antiviral responses are critical for modulating host-virus responses, and indeed, viruses have evolved strategies to antagonize this pathway. Encephalomyocarditis virus (EMCV) is an important zoonotic pathogen, which causes myocarditis, encephalitis, neurological disease, reproductive disorders, and diabetes in pigs. This study aims to understand how EMCV interacts with the IFN pathway. EMCV circumvents the type I IFN response by expressing proteins that antagonize cellular innate immunity. Here, we show that EMCV VP2 is a negative regulator of the IFN-β pathway. This occurs via the degradation of the MDA5-mediated cytoplasmic double-stranded RNA (dsRNA) antiviral sensing RIG-I-like receptor (RLR) pathway. We show that structural protein VP2 of EMCV interacts with MDA5, MAVS, and TBK1 through its C terminus. In addition, we found that EMCV VP2 could significantly degrade RLRs by the proteasomal and lysosomal pathways. For the first time, EMCV VP2 was shown to play an important role in EMCV evasion of the type I IFN signaling pathway. This study expands our understanding that EMCV utilizes its capsid protein VP2 to evade the host antiviral response. Encephalomyocarditis virus is an important pathogen that can cause encephalitis, myocarditis, neurological diseases, and reproductive disorders. It also causes huge economic losses for the swine industry worldwide. Innate immunity plays an important role in defending the host from pathogen infection. Understanding pathogen microorganisms evading the host immune system is of great importance. Currently, whether EMCV evades cytosolic RNA sensing and signaling is still poorly understood. In the present study, we found that viral protein VP2 antagonized the RLR signaling pathway by degrading MDA5, MAVS, and TBK1 protein expression to facilitate viral replication in HEK293 cells. The findings in this study identify a new mechanism for EMCV evading the host's innate immune response, which provide new insights into the virus-host interaction and help develop new antiviral approaches against EMCV.
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http://dx.doi.org/10.1128/JVI.01590-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094936PMC
February 2021

Host antiviral protein IFITM2 restricts pseudorabies virus replication.

Virus Res 2020 10 31;287:198105. Epub 2020 Jul 31.

Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China. Electronic address:

Pseudorabies virus (PRV) is one of the most destructive swine pathogens and leads to huge economic losses to the global pig industry. Type I interferons (IFNs) plays a pivotal role in the innate immune response to virus infection via induction of a series of interferon-stimulated genes (ISGs) expression. IFN-induced transmembrane (IFITM) proteins, a group of ISGs, are important host self-restriction factors, possessing a broad spectrum of antiviral effects. They are known confer resistance to a variety of RNA and DNA viruses. However, little is known about the role of IFITMs in PRV infection. In this study, we show that IFITM is crucial for controlling PRV infection and that IFITM proteins can interfere with PRV cell binding and entry. Furthermore, we showed that IFITM2-mediated inhibition of PRV entry requires the cholesterol pathway. Collectively, these results provide insight into the anti-PRV role of IFITM proteins and this inhibition possible associated with the change of cholesterol in the endosome, further underlying the importance of cholesterol in virus infection.
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http://dx.doi.org/10.1016/j.virusres.2020.198105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834200PMC
October 2020

Comparative studies on the interaction of nine flavonoids with trypsin.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Sep 4;238:118440. Epub 2020 May 4.

Grade 2018, School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.

In this study, the interaction between nine classic flavonoids (including baicalin, quercetin, myricetin, rutin, puerarin, daidzein, liquiritin and isoliquiritin) and trypsin was investigated by fluorescence spectroscopy and molecular modeling methods. The results reveal that all flavonoids can interact with trypsin to form flavonoid-trypsin complexes. The binding parameters obtained from the data at different temperatures indicate that all flavonoids can spontaneously bind with trypsin with one binding site. The binding constants of trypsin with nine classic flavonoids are in the following order as: baicalin > myricetin > rutin > isoliquiritin > hesperidin > puerarin > quercetin > daidzein > liquiritin. The interaction forces between flavonoids and trypsin may be electrostatic forces (except for rutin/puerarin/daidzein), hydrophobic interactions as well as van der Waals forces. Synchronous fluorescence spectroscopy shows that the interaction between flavonoids and trypsin changes the hydrophobicity of the microenvironment of tryptophan (Trp) residues. All flavonoids close to tyrosine (Tyr) residues but have no effect on the microenvironment around Tyr residues except for hesperidin and liquiritin. Molecular modeling displays that all flavonoids bind directly into trypsin cavity site and lead to a decrease in enzyme activity.
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http://dx.doi.org/10.1016/j.saa.2020.118440DOI Listing
September 2020

Gender difference in the association of coping styles and social support with psychological distress among patients with end-stage renal disease.

PeerJ 2020 26;8:e8713. Epub 2020 Mar 26.

Department of Social Medicine and Health Management, School of Public Health, Jilin University, Changchun, China.

Objectives: The study aimed to explore the association of coping styles and social support with psychological distress among patients with end-stage renal disease (ESRD) as well as gender differences in these associations.

Methods: A cross-sectional study of 769 ESRD patients was conducted at 25 hospitals in Qiqihar City, China. All information was collected using structured questionnaires that were self-administered by the patients. Patients' psychological health status, coping styles and perceived social support were measured using the 12-item General Health Questionnaire, the Medical Coping Modes Questionnaire and the Multidimensional Scale of Perceived Social Support. Student's test, analysis of variance (ANOVA) and binary logistic regression analysis were used for data analysis.

Results: A total of 72.3% of participants reported psychological distress, and the prevalence of distress was higher in females (77.0%) than in males (68.8%). The usage of the acceptance-resignation coping style was positively related to psychological distress (B = 0.459,  < 0.001). Social support level was negatively associated with psychological distress (B = -0.049,  < 0.001). The use of the confrontation style was negatively related to psychological distress in females (OR = 0.87, 95% CI [0.78-0.96]) but not in males (OR = 0.98, 95% CI [0.91-1.06]) ( for interaction term = 0.007).

Conclusions: Greater use of the acceptance-resignation style and lower social support are related to a higher risk of psychological distress. Greater use of the confrontation style is related to a lower risk of psychological distress in females but not in males.
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http://dx.doi.org/10.7717/peerj.8713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103200PMC
March 2020

The Associations of Family Functioning, General Well-Being, and Exercise with Mental Health among End-Stage Renal Disease Patients.

Psychiatry Investig 2020 Apr 8;17(4):356-365. Epub 2020 Apr 8.

Department of Social Medicine and Health Management, School of Public Health, Jilin University, Changchun, China.

Objective: This study aims to explore the relationships of family functioning, general well-being, and exercise with psychological distress. Furthermore, we investigated the special roles of general well-being and exercise on the association between family functioning and psychological distress.

Methods: Of 769 end-stage renal disease (ESRD) patients participated in the cross-sectional study which consisted of the 12-item General Health Questionnaire (GHQ-12), the Family APGAR Scales, and the General Well-Being Schedule. The collected data were analyzed using multiple linear regression analysis and path analysis.

Results: The prevalence of psychological distress was 72.3%. Family functioning, general well-being and exercise were associated factors of psychological distress (p<0.05). The indirect effect of family functioning on psychological distress was partially mediated by general well-being (Effect=-0.08, 95% CI=-0.11, -0.04). In addition, the effect of family functioning on general well-being was moderated by exercise (Index=-0.092, SE=0.033, 95% CI=-0.159, -0.029).

Conclusion: The prevalence of psychological distress among ESRD patients was high. Family functioning, general well-being and exercise were associated with psychological distress. Family functioning could affect psychological distress partially by affecting general well-being. Furthermore, exercise had a significant moderating effect on the relationship between family functioning and general well-being.
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http://dx.doi.org/10.30773/pi.2019.0204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176562PMC
April 2020

Alteration of liver immunity by increasing inflammatory response during co-administration of methamphetamine and atazanavir.

Immunopharmacol Immunotoxicol 2020 Jun 6;42(3):237-245. Epub 2020 Apr 6.

Department of Forensic Science, School of Basic Medical Science, Central South University, Changsha, Hunan, P.R. China.

Use of methamphetamine (METH) is prevalent among HIV-infected individuals. Previous research has shown that both METH and HIV protease inhibitors exert influences on mitochondrial respiratory metabolism and hepatic nervous system. This study aims to study the joint effect of METH and HIV protease inhibitors on hepatic immune function. Based on the differentially expressed genes obtained from RNA-seq of the liver from mouse model, the expression levels of CD48 and Macrophage Receptor with Collagenous Structure (MARCO) were examined using qRT-PCR and flow cytometry, and the expression and secretion of cytokines IL-1β, IL-6, IL-8, IL-10, IFN-γ, IFN-β, and TNF-α were determined using qRT-PCR and ELISA in THP-1-derived macrophages. Our results indicated that compared with the control group, CD48 molecules were significantly down-regulated by METH-atazanavir co-treatment, and the expression level of CD48 decreased as METH concentration increases. MARCO molecules were increased, especially at larger doses of METH and atazanavir treatment. In addition, in the presence of METH-atazanavir, the expression and secretion of a series of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-8 increased while the expression and secretion of anti-inflammatory cytokine IL-10 decreased. These results demonstrated that METH and atazanavir had a combined impact on the liver immunity, suggesting that the co-treatment could enhance inflammatory response and suppress NK cell activation CD48.
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http://dx.doi.org/10.1080/08923973.2020.1745829DOI Listing
June 2020

Enhanced production of questin by marine-derived HN4-13.

3 Biotech 2020 Feb 21;10(2):54. Epub 2020 Jan 21.

Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, 222005 China.

Questin has favorable applications. Fractional factorial design, Box-Behnken design, and response surface methodology were adopted to optimize the fermentation conditions of the marine-derived fungus, HN4-13, thereby enhancing questin production. Optimal fermentation conditions in a 500-mL conical flask with 200 mL of medium were 4% soluble starch, 0.9% beef extract, 4% NaCl, 0.05% NaHPO, pH 6, 2% inoculum size, and shaking at 28 ℃ and 160 rpm/min for 7 days. The production of questin can achieve 64.93 ± 4.55 mg/L, with no significant difference from the predicted value (66.27 mg/L). Thus, this optimized process of questin production is feasible. Such production is 17-fold higher than that of the basal Sabouraud's dextrose medium. Results indicate the potential of . HN4-13 in the large-scale production of questin through fermentation.
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http://dx.doi.org/10.1007/s13205-020-2067-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6974015PMC
February 2020

Transmembrane Protein 39A Promotes the Replication of Encephalomyocarditis Virus Autophagy Pathway.

Front Microbiol 2019 29;10:2680. Epub 2019 Nov 29.

Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, China.

Encephalomyocarditis virus (EMCV) causes encephalitis, myocarditis, neuropathy, reproductive disorders, and diabetes in animals. EMCV is known to induce cell autophagy; however, the molecular mechanisms underlying this remain unclear. Here, we show that the type III-transmembrane protein, transmembrane protein 39A (TMEM39A), plays a critical role in EMCV replication. We showed that EMCV GS01 strain infection upregulated TMEM39A expression. Importantly, EMCV induced autophagy in a range of host cells. The autophagy chemical inhibitor, 3-MA, inhibited EMCV replication and reduced TMEM39A expression. This is the first study demonstrating TMEM39A promoting the replication of EMCV autophagy. Overall, we show that TMEM39A plays a positive regulatory role in EMCV proliferation and that TMEM39A expression is dependent on the autophagy pathway.
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http://dx.doi.org/10.3389/fmicb.2019.02680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901969PMC
November 2019

Electromyography activities in patients with lower lumbar disc herniation.

J Back Musculoskelet Rehabil 2020 ;33(4):589-596

Department of Physical Medicine and Rehabilitation, Qilu Hospital of Shandong University, Jinan, China.

Background: Lumbar disc herniation (LDH) can affect lower limb muscle function resulting in an abnormal gait. This study aims to use surface electromyography (SEMG) to evaluate patients with L4/L5 and L5/S1 LDH throughout muscle movement.

Methods: Twenty L4/L5 LDH patients (L5 Group), twenty L5/S1 LDH patients (S1 Group), and twenty healthy controls (Healthy) were recruited for the study. SEMG of bilateral tibialis anterior (TA) and lateral gastrocnemius (LG) muscles of patients were recorded using the DELSYS Wireless EMG System (TrignoTM Wireless Systems, Delsys Inc., USA). Root-mean-square (RMS), mean power frequency (MPF), and median frequency (MF) were compared between bilateral limbs in each participant.

Results: Reduced MPF and MF was found in TA measurements of the L5 Group and LG measurements of the S1 Group. The MPF and MF of the TA of symptomatic limbs of the L5 Group were reduced when compared to asymptomatic limbs (p= 0.006, p= 0.012, p< 0.05), and there were no significant differences in LG measurements (p> 0.05). The LG MPF and MF of the S1 Group in symptomatic limbs were reduced when compared to asymptomatic limbs (p= 0.006, p= 0.017, p< 0.05), and there were no significant differences in TA measurements (p> 0.05). Although there were no significant differences in RMS between bilateral limbs of the L5 and S1 Groups, we found some changes in RMS curves. First, compared to asymptomatic limbs of L4/L5LDH patients, β-peaks in the TA of symptomatic limbs appeared earlier. Second, two peaks in the LG of symptomatic limbs were found in L5/S1 LDH patients.

Conclusion: TA is affected in patients with LDH of L4/L5, and LG is affected in patients with LDH of L5/S1. As demonstrated, SEMG can identify LDH-related muscle dysfunction.
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http://dx.doi.org/10.3233/BMR-181308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7458508PMC
December 2020

Comparative study of striatum GABA concentrations and magnetic resonance spectroscopic imaging in Parkinson's disease monkeys.

BMC Neurosci 2019 08 8;20(1):42. Epub 2019 Aug 8.

Department of Radiology, The First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Nanning, 530021, Guangxi Province, China.

Background: Parkinson's disease is a progressive degenerative nervous system disease. Recent studies have shown that secondary changes in the GABA system play directly affect the pathogenesis of PD. There is still much debate about GABA concentrations because currently, GABA concentrations in the brain tissue are obtained indirectly by measuring its concentration in the blood and cerebrospinal fluid. These results are unreliable. Magnetic resonance spectroscopy (MRS) is the only noninvasive method for evaluating the concentration of metabolites in living brain tissue and has been widely applied in research and clinical practice. In addition, combining MEGA-PRESS technology with LCModel software for quantitative GABA measurements is largely recognized. At present, the PD monkeys model in primates has been increasingly proficient. Primates are more similar to humans in terms of brain structure and function than other animals. However, 3.0 T MRS studies involving the PD monkey model to measure metabolites in living subjects with PD are still rare. The study was performed at 3.0 T MRI with control monkeys and PD monkeys that were injected methyl-phenyl-tetrahydropyridine (MPTP) in one side of common carotid artery before and 3 months after successful model establishment to measure GABA concentrations in the bilateral striatum. Behavioral observations were performed for all animals, and the behavioral score was recorded. After 3 months, the GABA concentration in the bilateral striatum was measured in both groups by high-performance liquid chromatography (HPLC). The data obtained from magnetic resonance spectroscopy (MRS) were compared with the actual measured GABA concentrations in tissues isolated from the corresponding regions, and their correlations with the behavior score were analyzed. The research objectives are to investigate the changes of γ-aminobutyric acid (GABA) concentration in the bilateral striatum of monkeys with Parkinson's disease (PD) and the value of quantitatively measuring its concentration by noninvasive 3.0 T spectroscopy.

Results: (1) The MRS results showed that the GABA concentration in the injured side of the striatum of the PD monkeys was higher than in the contralateral side, but the difference was not statistically significant (P = 0.154). Compared with that the blank control group, the GABA concentration in the striatum of the PD monkeys increased, but there was no difference between the groups (P = 0.381; P = 0.425). (2) The GABA concentration that determined from the isolated specimens by HPLC in the injured side of the striatum of the PD monkeys was significantly higher than that in the contralateral side (P < 0.01). Compared with the blank control group, the PD monkeys had higher GABA concentrations in both sides of the striatum, and there was a significant difference in the lesion side (P = 0.004), while there was a non-significant difference in the contralateral side (P = 0.475). (3) The mean GABA concentration in the injured striatum of PD monkeys determined by MRS was not significantly correlated with the behavioral score (r = 0.146, P = 0.688). The mean GABA concentration in the injured striatum determined from the isolated specimens was positively correlated with the behavioral score in the same period (r = 0.444, P = 0.038).

Conclusion: The GABA concentration in the injured striatum of PD monkeys is increased and positively correlated with behavioral changes. Validity of noninvasive 3.0 T MRS to detect PD neurotransmitter changes is limited.
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http://dx.doi.org/10.1186/s12868-019-0522-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686405PMC
August 2019

Clinical characteristics of and treatment protocol for trapped temporal horn following resection of lateral ventricular trigone meningioma: a single-center experience.

J Neurosurg 2019 02;132(2):481-490

1Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing; and.

Objective: Trapped temporal horn (TTH) is a rare subtype of loculated hydrocephalus that is often managed surgically. The natural history of TTH is not well understood, and there are few data on the outcomes of conservative management of this condition. The aim of this study was to analyze the clinical features and outcomes of conservatively and surgically managed cases of TTH.

Methods: The authors retrospectively reviewed the clinical data for 19 consecutive cases of TTH that developed after microsurgical resection of lateral ventricular trigone meningioma between 2011 and 2015.

Results: The 19 cases involved 6 male and 13 female patients (mean age [± SD] 39.9 ± 13.8 years). The mean time interval from tumor resection to onset of TTH was 3.2 ± 3.0 months (range 3 days-10 months). Symptoms of intracranial hypertension were the most common complaints at presentation. The mean Karnofsky Performance Scale (KPS) score at onset was 52.1 ± 33.3 (range 10-90). Midline shift was observed in 15 cases (78.9%), and the mean amount of midline shift was 6.0 ± 4.8 mm (range 0-15 mm). Eleven cases (57.9%) were managed with surgical intervention, while 8 cases (42.1%) were managed conservatively. All patients (100%) showed improved clinical status over the course of 4.8 ± 1.0 years (range 2.8-6.3 years) of follow-up. The mean KPS score at last follow-up was 87.9 ± 11.3 (range 60-100). Eighteen patients (94.7%) showed signs of radiographic improvement, and 1 patient (5.3%) exhibited stable size of the temporal horn. Significant differences were observed between the surgical and nonsurgical cohorts for the following variables: KPS score at onset, presence of intracranial hypertension, and midline shift. The mean KPS score at onset was greater (better) in the nonsurgical group than in the surgical group (82.5 ± 8.9 vs 30 ± 25.7, p = 0.001). A greater proportion of patients in the surgical group presented with symptoms of intracranial hypertension (81.8% vs 0%, p = 0.001). The extent of midline shift was greater in the surgical group than in the nonsurgical group (9.0 ± 3.8 mm vs 2.0 ± 2.4 mm, p = 0.001).

Conclusions: The majority of patients with TTH presented in a delayed fashion. TTH is not always a surgical entity. Spontaneous resolution of TTH may be under-reported. Conservative management with clinical and radiological follow-up is effective in selected patients.
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http://dx.doi.org/10.3171/2018.11.JNS182710DOI Listing
February 2019

Risk factors and control of seizures in 778 Chinese patients undergoing initial resection of supratentorial meningiomas.

Neurosurg Rev 2020 Apr 15;43(2):597-608. Epub 2019 Feb 15.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No.119 South 4th Ring West Road, Fengtai District, Beijing, 100070, China.

This retrospective study explored the risk factors for the occurrence of seizures in the pre- and postoperative period in patients undergoing supratentorial meningiomas surgery to investigate those who are likely to benefit from prophylactic antiepileptic drugs (AEDs). We reviewed the medical records of 778 supratentorial meningiomas patients who were operated at our institution between 2011 and 2012. A total of 100 (12.9%) patients experienced preoperative seizures; 41 patients (5.3%) experienced postoperative in-hospital seizures, and 91 (13.5%, n = 673) patients experienced postoperative seizures after discharge. Multivariate analysis revealed that motor cortex involvement (odds ratio [OR] 3.243, P < 0.001) and peritumoral edema ≥ 1 cm (OR 3.936, P < 0.001) were significant risk factors of preoperative seizures. Whereas presenting with headache (OR 0.259, P < 0.001) and age ≥ 55 years at surgery (OR 0.514, P = 0.009) showed decreased incidence of preoperative seizures. The involvement of motor cortex (OR 3.290, P = 0.003), postoperative Karnofsky Performance Scale (KPS) ≤ 70 (OR 5.389, P < 0.001), preoperative seizure (OR 4.003, P < 0.001), and occurrence of any medical/surgical complication (OR 3.925, P = 0.001) were significant risk factors for postoperative in-hospital seizures. Postoperative seizures after discharge were associated with tumor maximal diameter ≥ 3.5 cm (OR 1.903, P = 0.022), preoperative seizures (OR 4.350, P < 0.001), postoperative in-hospital seizures (OR 6.385, P < 0.001), and tumor recurrence/progression (OR 7.642, P < 0.001). The probability of seizure freedom in the 5-year follow-up was roughly 59% among patients with preoperative seizures, and 87% among patients without preoperative seizures. Cox regression analysis showed that tumor recurrence/progression (relative risk 2.987, 95% CI 1.517, 5.879, P = 0.002) was the only predictor of postoperative seizures in patients without a history of preoperative epilepsy. The use of postoperative prophylactic antiepileptic drug (AED) did not reduce the incidence of seizures in our analysis. Understanding the risk factors for seizures might help clinicians to predict their occurrence and develop effective anti-epileptic treatment strategies. Further prospective randomized controlled trials are needed to determine the risk factors for seizures and the efficacy of AED prophylaxis.
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http://dx.doi.org/10.1007/s10143-019-01085-5DOI Listing
April 2020

Characteristic features and proposed classification in 69 cases of intracranial microcystic meningiomas.

Neurosurg Rev 2019 Jun 2;42(2):443-453. Epub 2018 May 2.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 6 TiantanXili, Dongcheng District, Beijing, 100050, China.

Microcystic meningioma (MM) is a rare subtype of intracranial meningiomas, with clinical and radiologic features not well characterized in the literature. Based on our experience, we propose a classification system of intracranial MMs. We reviewed the medical records, radiographic studies, and operative notes of a group of consecutive patients with intracranial MM. The mean age of the 69 patients was 46.8 ± 10.6 years (range, 21-75 years). Three types of intracranial MMs could be identified. Type 1 MMs presented as a solid lesion, hypointense or isointense on T1WI, hyperintense on T2WI, and homogeneous or heterogeneous enhancement, and were found in 43 patients (67.2%). Type 2 MMs represented signals similar to CSF both on T1WI and T2WI, and faint reticular enhancement with marginal enhancement, and these were found in 7 patients (10.9%). Type 3 MMs consisted of cystic-solid or cystic lesion and were found in 14 patients (21.9%). Significant differences were observed among the different types of MMs for the following variables: sex, presence of severe peritumoral brain edema (PTBE), and extent of tumor resection. Females were found in all of patients with type 2 MMs, but were only 35.7% of those with type 3 MMs (P = 0.018). Severe PTBEs were more common among patients with type 1 MMs (55.8%) than among those with type 2 (14.3%) and type 3 MMs (14.3%) (P = 0.007). Type 1 MMs (97.7%) were associated with a significantly higher rate of gross total resection compared with the other two types (71.4 and 78.6%) (P = 0.019). Total length of hospital stay after craniotomy ranged from 4 to 30 days (median, 8 days). There were no significant differences in progression-free survival among the three types of MMs (P = 0.788). The current classification identifies three distinct types of intracranial MM based on their radiological findings and growth patterns. The type 1 MMs are more commonly associated with severe PTBE. Type 2 and Type 3 MMs have a higher predilection towards parasaggital location with venous involvement and therefore have a lower rate of gross total resection.
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http://dx.doi.org/10.1007/s10143-018-0982-9DOI Listing
June 2019

Binding behaviors and kinetics studies on the interaction of silver nanoparticles with trypsin.

Int J Biol Macromol 2018 Jul 29;114:836-843. Epub 2018 Mar 29.

Grade 2017, Clinical Pharmacy, School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.

The interaction of nanoparticles (NPs) with proteins is a topic of high relevance for the medical application of nanomaterials. In this study, a comprehensive investigation was performed to clarify the binding mechanism, adsorption isotherms and kinetics of the interaction between silver nanoparticles (AgNPs) and trypsin. The experimental results indicate that the binding of AgNPs to trypsin seems to be a static quenching mechanism. Thermodynamic analysis reveals that AgNPs binding to trypsin is synergistically driven by enthalpy and entropy, and the major driving forces are hydrophobic and electrostatic interactions. The adsorption of trypsin on AgNPs was analyzed by Langmuir and Freundlich models, suggesting that the equilibrium adsorption data fit well with Freundlich model. The kinetics of adsorption data were modeled using the pseudo-first-order and pseudo-second-order kinetic equations. The results indicate that a pseudo-second-order kinetic equation describes better. The conformational change at the secondary structural level of trypsin induced by AgNPs was investigated with the circular dichroism (CD) measurements and no obvious changes in trypsin secondary structural elements are observed. These fundamental works will provide some new insights into the safe and effective application of AgNPs in biological and medical areas.
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http://dx.doi.org/10.1016/j.ijbiomac.2018.03.172DOI Listing
July 2018

Exploring the interaction of silver nanoparticles with pepsin and its adsorption isotherms and kinetics.

Chem Biol Interact 2018 Apr 10;286:52-59. Epub 2018 Mar 10.

Grade 2017, Clinical Pharmacy, School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, PR China.

The interaction of nanoparticles (NPs) with proteins is a topic of high relevance for the medical application of nanomaterials. In the study, a comprehensive investigation was performed for the binding properties of silver nanoparticles (AgNPs) to pepsin. The results indicate that the binding of AgNPs to pepsin may be a static quenching mechanism. Thermodynamic analysis reveals that AgNPs binds to pepsin is synergistically driven by enthalpy and entropy, and the major driving forces are hydrophobic and electrostatic interactions. Synchronous fluorescence spectroscopy shows that AgNPs may induce microenvironmental changes of pepsin. The hydrophobicity of Trp is increased while the hydrophility of Tyr is increased. The adsorption of pepsin on AgNPs was analyzed by Langmuir and Freundlich models, suggesting that the equilibrium adsorption data fit well with Freundlich model. The equilibrium adsorption data were modeled using the pseudo-first-order and pseudo-second-order kinetic equations. The results indicate that pseudo-second-order kinetic equation better describes the adsorption kinetics. The study provides an accurate and full basic data for clarifying the binding mechanism, adsorption isotherms and kinetic behaviors of AgNPs with pepsin. These fundamental works will provide some new insights into the safe and effective application of AgNPs in biological and medical areas.
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http://dx.doi.org/10.1016/j.cbi.2018.03.004DOI Listing
April 2018

Construction, expression, and characterization of a single-chain variable fragment (ScFv) antibody targeting to the encephalomyocarditis virus.

J Med Virol 2018 07 30;90(7):1184-1191. Epub 2018 Mar 30.

The Key Bio-Engineering and Technology Laboratory of SEAC, Northwest Minzu University, Lanzhou, PR China.

Encephalomyocarditis virus (EMCV) is as a potential zoonotic agent with a wide host range. Here, applying gene splicing by overlap extension PCR (SOE-PCR), we describe a simple method for producing single-chain variable fragment (scFv) antibody against EMCV that configurates in the orientation of VH-(GGGGS) -VL. DNA template was resverse transcribed by total RNA that derived from hyperimmunized antibody positive mice spleen after inoculation inactivated EMCV-PV21 as antigen. Using the degenerate primers designed for the variable regions of IgG of murine antibody, the 417 bp of gene encoding VH-linker (VHL) and 360 bp of gene encoding linker-VL (LVL) of the anti-EMCV was individually amplified from DNA template by PCR, repectively. The 762 bp gene encoding anti-EMCV scFv was constructed by SOE-PCR when the mixed VHL and LVL genes were used as the template. The amplified gene subcloned into pGEX-6P1 to yield pGEX-6P1/EMCV-scFv. Recombinant vector transformed into the Escherichia coli BL21 (DE3) and a 53 KDa GST-scFv fusion protein was obtained by SDS-PAGE electrophoresis. Animal experiment results showed that the pretective rate of mice in group A which challenged 500 μL 10 TCID EMCV per mouse for 7 d post-inoculation scFv 3 d (0.5 mg purified recombinant scFv per mouse) was 91.67% (11/12). The serum anti-EMCV antibody titer in group A mice was most significantly higher than that in positive control mouse (P < 0.01), coversely the serum relative mRNA copies were significantly lower than that in positive control mouse (P < 0.05). These findings indicated that recombinant anti-EMCV scFv has remarkable anti-EMCV effect in mice.
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http://dx.doi.org/10.1002/jmv.25065DOI Listing
July 2018

Risk of cancer death by comorbidity severity and use of adjuvant chemotherapy among women with locoregional breast cancer.

J Geriatr Oncol 2018 05 23;9(3):214-220. Epub 2017 Nov 23.

Louisiana State University Health Sciences Center, 2020 Gravier Street, New Orleans, LA 70112, USA.

Objectives: To examine the associations of comorbidity and chemotherapy with breast cancer- and non-breast cancer-related death.

Materials And Methods: Included were women with invasive locoregional breast cancer diagnosed in 2004 from seven population-based cancer registries. Data were abstracted from medical records and verified with treating physicians when there were inconsistencies and missing information on cancer treatment. Comorbidity severity was quantified using the Adult Comorbidity Evaluation 27. Treatment guideline concordance was determined by comparing treatment received with the National Comprehensive Cancer Network guidelines. Kaplan-Meier method and multivariable Cox proportional hazards regressions were employed for statistical analyses.

Results: Of 5852 patients, 76% were under 70years old and 69% received guideline concordant adjuvant chemotherapy. Comorbidity was more prevalent in women age 70 and older (79% vs. 51%; p<0.001). After adjusting for tumor characteristics and treatment, severe comorbidity burden was associated with significantly higher cancer-related mortality in older patients (Hazard Ratio [HR]=2.38, 95% CI 1.08-5.24), but not in younger patients (HR=1.78, 95% CI 0.87-3.64). Among patients receiving guideline adjuvant chemotherapy, cancer-related mortality was significantly higher in older patients (HR=2.35, 95% CI 1.52-3.62), and those with severe comorbidity (HR=3.79, 95% CI 1.72-8.33).

Conclusions: Findings suggest that, compared to women with no comorbidity, patients with breast cancer age 70 and older with severe comorbidity are at increased risk of dying from breast cancer, even after adjustment for adjuvant chemotherapy and other tumor and treatment differences. This information adds to risk-benefit discussions and emphasizes the need for further study of the role for adjuvant chemotherapy in these patient groups.
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http://dx.doi.org/10.1016/j.jgo.2017.11.004DOI Listing
May 2018

Fluorescence spectroscopic analysis of the interaction of papain and bromelain with l-ascorbic acid, α-tocopherol, β-carotene and astaxanthin.

Int J Biol Macromol 2018 Feb 6;107(Pt A):144-156. Epub 2017 Sep 6.

Grade 2012, Class 25, Clinical speciality, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.

In this study, the interaction between four classic dietary antioxidants (including l-ascorbic acid, α-tocopherol, β-carotene and astaxanthin) and papain/bromelain was investigated by fluorescence spectroscopy. The results show that the quenching mechanisms are all static quenching at lower concentrations of antioxidants, but at higher concentrations of antioxidants, predominantly by the "sphere of action" quenching mechanisms. The binding processes of the four antioxidants to papain/bromelain are all synergistically driven by enthalpy and entropy, and the major driving forces are electrostatic effect and hydrophobic interactions. The binding constants of papain/bromelain with the four antioxidants are in the following order as: astaxanthin-papain >β-carotene-papain > astaxanthin-bromelain >l-ascorbic acid-papain >l-ascorbic acid-bromelain >β-carotene-bromelain >α-tocopherol-papain >α-tocopherol-bromelain. Synchronous fluorescence spectroscopy shows the interaction between l-ascorbic acid/β-carotene/astaxanthin and papain/bromelain decreases the hydrophobicity of the microenvironment of tryptophan (Trp) and tyrosine (Tyr) residues. The hydrophobicity of Trp is increased while the hydrophility of Tyr is increased in the presence of α-tocopherol.
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http://dx.doi.org/10.1016/j.ijbiomac.2017.08.150DOI Listing
February 2018

A modified nonmonotone BFGS algorithm for unconstrained optimization.

J Inequal Appl 2017 9;2017(1):183. Epub 2017 Aug 9.

Guangxi Colleges and Universities Key Laboratory of Mathematics and Its Applications, College of Mathematics and Information Science, Guangxi University, Nanning, Guangxi P.R. China.

In this paper, a modified BFGS algorithm is proposed for unconstrained optimization. The proposed algorithm has the following properties: (i) a nonmonotone line search technique is used to obtain the step size [Formula: see text] to improve the effectiveness of the algorithm; (ii) the algorithm possesses not only global convergence but also superlinear convergence for generally convex functions; (iii) the algorithm produces better numerical results than those of the normal BFGS method.
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http://dx.doi.org/10.1186/s13660-017-1453-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550551PMC
August 2017

ANTIOXIDANT AND ANTI-INFLAMMATORY EFFECTS OF RHAMNAZIN ON LIPOPOLYSACCHARIDE-INDUCED ACUTE LUNG INJURY AND INFLAMMATION IN RATS.

Afr J Tradit Complement Altern Med 2017 5;14(4):201-212. Epub 2017 Jun 5.

Department of respiratory medicine, Changzhou Jintan District People's Hospital, Changzhou, Jiangsu 213200, China.

Background: Acute Lung Injury (ALI) results into severe inflammation and oxidative stress to the pulmonary tissue. Rhamnazin is a natural flavonoid and known for its antioxidant and anti-inflammatory properties.

Materials And Methods: The antioxidative and anti-inflammatory properties rhamnazin were tested for protection against the acute lung injury. We investigated whether rhamnazin improves the lipopolysaccharide (LPS)-induced ALI in an animal model (rat). We also studied the probable molecular mechanism of action of rhamnazin. Rhamnazin was injected intraperitoneally (i.p.) (5, 10 and 20 mg/kg) two days before intratracheal LPS challenge (5mg/kg). The changes in lung wet-to-dry weight ratio, LDH activity, pulmonary histopathology, BALF protein concentration, MPO activity, oxidative stress, cytokine production were estimated.

Results: The results showed a significant attenuation of all the inflammatory parameters and a marked improvement in the pulmonary histopathology in the animal groups pretreated with rhamnazin. The rhamnazin pretreated group also showed activation of Nrf2 pathway and attenuation of ROS such as HO, MDA and hydroxyl ion. These results indicated that rhamnazin could attenuate the symptoms of ALI in rats due to its strong antioxidant and anti-inflammatory properties.

Conclusion: The results strongly demonstrated that rhamnazin provides protection against LPS-induced ALI. The underlying mechanisms of its anti-inflammatory action may include inhibition of Nrf2 mediated antioxidative pathway.
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http://dx.doi.org/10.21010/ajtcam.v14i4.23DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5471467PMC
August 2017

Transcriptional profiling of host cell responses to encephalomyocarditis virus (EMCV).

Virol J 2017 03 4;14(1):45. Epub 2017 Mar 4.

The Key Bio-engineering and Technology Laboratory of SEAC, Northwest Minzu University, No. 1 Xibeixincun, Lanzhou, 730030, People's Republic of China.

Backgroud: Encephalomyocarditis virus (EMCV) has been discovered on pig farms worldwide and can cause myocarditis in piglets and reproductive failure in sows. However, little is known about the host transcriptional responses to infection and host-pathogen interactions.

Methods: In this study, transcription profiling was performed by Illumina RNA-Sequencing (RNA-seq) to identify EMCV induced differentially expressed genes in BHK-21 cells at serial time points (12, 24, and 30 h post infection (hpi)), using mock infected cells as control.

Results: We identified 237, 241, and 207 differentially expressed genes (DEGs) respectively, majority of which were up-regulated. A large number of DEGs clustered into host defense, cellular signaling and metabolism categories. Moreover, short time series expression analysis revealed that 12 hpi was an important time point for expression change, indicating host virus resistance.

Conclusions: This RNA-seq analysis provides the first data for understanding the network of virus host interactions under EMCV infection in vitro, and for identifying host components which involved in the virus infection course.
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http://dx.doi.org/10.1186/s12985-017-0718-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336634PMC
March 2017

Clinical Features, Radiologic Findings, and Surgical Outcomes of 65 Intracranial Psammomatous Meningiomas.

World Neurosurg 2017 Apr 27;100:395-406. Epub 2017 Jan 27.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. Electronic address:

Objective: Intracranial psammomatous meningiomas (PMs) are rarely reported due to their extremely low incidence. Knowledge about intracranial PMs is therefore poor. This study aimed to analyze the incidence, clinical features, radiologic findings, and surgical outcomes of intracranial PMs.

Methods: Approximately 5651 intracranial meningiomas were surgically treated at Beijing Tiantan Hospital between November 2011 and December 2015. The authors identified all cases in which patients underwent resection of an intracranial tumor that was found to be a PM on pathologic examination and analyzed the data from these cases.

Results: PMs accounted for 1.15% of the entire series of intracranial meningiomas. Sixty-two (95.4%) of these 65 intracranial PMs were female. The median maximal tumor diameter was 2.8 cm (range, 1.4-8.4 cm). On computed tomography scan, all PMs (23/23) showed various calcification. Gross total resection was achieved in 56 cases, near total resection in 5, subtotal resection in 3, and partial resection in 1. Seventeen (26.2%) of the patients experienced new or exacerbated deficits immediately after operation. Three patients (17.6%, 3/17) got alleviation when assessed at last follow-up. Eleven patients (16.9%) experienced additional surgical complications. At the time of discharge, Karnofsky Performance Scale (KPS) score and neurologic function showed improvement in 50 cases (76.9%) and worsening in 15 cases (23.1%). At a median follow-up of 37 months (range, 7-56 months), 1 patient had experienced tumor regrowth 50 months after surgery and the 4-year progression-free survival rates were 100.0%. No patient died of recurrence. At the latest follow-up, the KPS score showed improvement in 56 cases (86.2%) and worsening in 9 cases (13.8%). Tumor located in the skull base is significantly associated with an increased risk of worsened long-term outcomes. Preoperative KPS score <90, peritumoral brain edema (PTBE), and hypointensity on T2-weighted imaging had a statistically significant effect on the extent of tumor resection.

Conclusions: Intracranial PM is a rare subtype of meningioma with marked female predominance and low tendency toward growth and recurrence. Most intracranial PMs exhibit highly calcified imaging characteristics, particularly as seen by computed tomography. Microsurgical treatment of skull base PM remains a formidable challenge due to the hard tumor consistency and critical neurovascular structure encasement. It is unnecessary for radical tumor resection at the cost of severe neurologic deficits.
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http://dx.doi.org/10.1016/j.wneu.2017.01.056DOI Listing
April 2017