Publications by authors named "Xiang Xu"

577 Publications

Chlorin e6 mediated photodynamic therapy triggers resistance through ATM-related DNA damage response in lung cancer cells.

Photodiagnosis Photodyn Ther 2021 Nov 22:102645. Epub 2021 Nov 22.

Department of Respiratory, Taihe Hospital of Shiyan, Hubei University of Medicine, Renmin road No. 30, Shiyan, Hubei, 442000, China; School of Basic Medical Sciences, Hubei University of Medicine, Renmin road No. 30, Shiyan, Hubei, 442000, China; Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, Renmin road No. 30, Shiyan, Hubei, 442000, China. Electronic address:

Objective: Photodynamic therapy (PDT) has emerged as a promising strategy in the treatment of malignant tumors due to its high selectivity, non-toxicity, and non-invasiveness. However, PDT can also induce DNA damage and subsequent repair response, which may reduce the efficacy of PDT. In the present study, we sought to explore the effect of chlorin e6 (Ce6)-mediated PDT on DNA damage and DNA damage response (DDR) in lung cancer cells. In addition, the effect of PDT combined with ATM inhibitor on molecules of DDR and the possibility of improving the efficacy of PDT were further investigated.

Materials And Methods: In the in vitro study, lewis cells were submitted to Ce6 treatment (2, 4, 8, 16, 32 μg/mL). To determine the concentration of Ce6, uptake and toxicity of Ce6 mediated PDT were detected using flow cytometry (FACS), Confocal microscopy, and CCK-8. In the subsequent research, 8 μg/mL of Ce6 was the treatment condition for inducing PDT. The different post-irradiation placement times were further grouped under this condition (2, 4, 6, 12 h). Cellular reactive oxygen species (ROS), damage of DNA were measured by DCFH-DA probe, comet assay respectively. Then the expression of p-ATM, p53, and γ-H2A.X proteins related to DNA damage response, was detected by WB. The efficacy of Ce6 induced PDT was also demonstrated by Annexin-V/PI staining as well as the expression of PCNA, cleaved-caspase-3. On this basis, ATM inhibitor was applied to treat lewis cells combined with Ce6 (2, 4 h) to investigate whether the efficacy of PDT induced by Ce6 can be improved after the ATM-related DDR was blocked. The cell viability, apoptosis, and expression of associated proteins were assayed.

Results: At 2-4 h after PDT treatment, ROS was dramatically elevated in lewis cells, DNA double-strand breaks (DDSB) occurred, as well as up-regulation of DDR proteins γ-H2A.X, p-ATM, and p53. At the same time, lewis cells did not undergo significant apoptosis. After ATM inhibition, the DDR was significantly blocked within 2-4 hours after Ce6 induced PDT, along with a pronounced decrease in cell viability followed by a prominent increase of apoptosis.

Conclusion: Ce6-mediated PDT generates ROS in a short period time, thus inducing DNA damage, ATM-related DDR as well as promoting resistance of lung cancer cells to PDT. Combining ATM inhibitor with PDT could effectively inhibit the DDR induced by PDT, thereby enhancing the efficacy. This study reveals a new resistance mechanism of PDT and proposes an intervention strategy.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102645DOI Listing
November 2021

Hydration and plasticization effects of maltodextrin on the structure and cooking quality of extruded whole buckwheat noodles.

Food Chem 2021 Nov 16:131613. Epub 2021 Nov 16.

College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, China. Electronic address:

In order to improve the structure and cooking quality of extruded whole buckwheat noodles (EWBN), maltodextrin (MD), the homologous substances of starch, was added to buckwheat flour to prepare the EWBN. Hydrogen bonds formed between MD and buckwheat starch molecules and the crystallinity of EWBN decreased as determined by FT-IR and X-ray diffraction, which indicated plasticization effects of MD on buckwheat starch. The content of tightly bound water first increased and then decreased with the increasing amount of MD and the cooking time of EWBM decreased from 5.4 to 3.1 min due to the hydration effects of MD. The cooking loss first decreased and then increased, and showed a minimum value of 9.22% when adding 1 wt% of MD. For texture properties, the hardness, stickiness, chewiness and elongation at break of EWBN first increased and then decreased with the addition of MD, and all reached the maximum value at 3 wt% of MD. These findings showed the potential of adding MD, especially at the appropriate concentration, for improving structure and cooking quality of EWBN.
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http://dx.doi.org/10.1016/j.foodchem.2021.131613DOI Listing
November 2021

Three species of hackled-orb web spider genus Miagrammopes from China (Araneae, Uloboridae).

Zootaxa 2021 Jul 22;5004(4):564-576. Epub 2021 Jul 22.

State Key Laboratory of Developmental Biology of Freshwater Fish, The National Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Normal University, Changsha 410081, Hunan, China.

Three species of the genus Miagrammopes O. Pickard-Cambridge, 1870 are described from China, including two new species, M. rutundus Liang Xu, n. sp. from Guangxi Zhuang Autonomous Region and M. auriculatus Cai Xu, n. sp. from Tibet Autonomous Region, and one known species, M. bifurcatus Dong, Yan, Zhu Song, 2004. The female of M. bifurcatus is described for the first time. Both detailed illustration and a distribution map of the three species are provided.
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http://dx.doi.org/10.11646/zootaxa.5004.4.5DOI Listing
July 2021

New species of the pirate spider genus Mimetus Hentz, 1832 from China with a cladistic hypothesis on their phylogenetic placement (Araneae, Mimetidae).

Zootaxa 2021 Aug 11;5020(1):1-30. Epub 2021 Aug 11.

State Key Laboratory of Developmental Biology of Freshwater Fish, The National Local Joint Engineering Laboratory of Animal Peptide Drug Development, Hunan Normal University, Changsha 410081, Hunan, China .

Five new species of mimetid spiders from China are described: Mimetus subulatus n. sp., M. clavatus n. sp., M. dentatus n. sp., M. niveosignatus n. sp. and M. uncatus n. sp. The phylogenetic placement of these new species is inferred based on a cladistic analysis of an expanded version of the morphological dataset of Benavides and Hormiga (2020). The new species form a clade that can be distinguished from other Mimetus species by the presence of a subtegular apophysis between paracymbium and subtegulum, pilose cuticular projections on the membranous part of the conductor in the male palp and by a bicameral structure of the spermathecae in females. The new species are part of a clade that includes Mimetus syllepsicus Hentz, 1832, the type species of the genus. The genus Mimetus as currently circumscribed is not monophyletic, as the clade that includes all the Mimetus species also includes the genera Australomimetus Heimer, 1986 and Anansi Benavides and Hormiga, 2017, corroborating the results of Benavides and Hormiga (2020).
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http://dx.doi.org/10.11646/zootaxa.5020.1.1DOI Listing
August 2021

Porous Carbon Nanofibers with Heteroatoms Doped by Electrospinning Exhibit Excellent Acetone and Carbon Dioxide Adsorption Performance: The Contributions of Pore Structure and Functional Groups.

ACS Omega 2021 Nov 4;6(45):30716-30725. Epub 2021 Nov 4.

School of Energy Science and Engineering, Central South University, Changsha 410083, Hunan, China.

Rich chemical properties and a well-developed pore structure are the key factors of porous materials for gas storage. Herein, rich heteroatom-doped porous carbon nanofibers (UK-X) with a large surface area were prepared by electrospinning followed by potassium hydroxide (KOH) activation. Low-cost urea was chosen as the nitrogen source and structural guiding agent. UK-X have a high specific surface area (628-2688 m g), excellent pore volume (0.468-1.571 cm g), and abundant nitrogen (2.5-12.8 atom %) and oxygen (4.5-12.5 atom %) contents. Acetone and carbon dioxide were used as target adsorbents to evaluate the adsorption properties of UK-X by experiments. These UK-X exhibit excellent adsorption performance (260.03-955.74 mg g, 25 °C, 18 kPa) and multilayer adsorption (the adsorption layer number > 2) for acetone, which is mainly attributed to the large specific surface area and pore volume. Besides this, the carbon dioxide uptake reached 2.73-3.34 mmol g at 25 °C. This was attributed to the combination of high nitrogen-oxygen contents and microporous structure. Furthermore, UK-X show the desirable repeatability. This study provides a new direction for the preparation of heteroatom-doped porous carbon nanofibers, which will be a promising material for gas adsorption.
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http://dx.doi.org/10.1021/acsomega.1c04618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600650PMC
November 2021

Novel Targets in a High-Altitude Pulmonary Hypertension Rat Model Based on RNA-seq and Proteomics.

Front Med (Lausanne) 2021 3;8:742436. Epub 2021 Nov 3.

Laboratory of Translational Medicine, Medical Innovation Research Division of Chinese PLA General Hospital, Beijing, China.

High-altitude pulmonary hypertension (HAPH) is a complication arising from an inability to acclimatize to high altitude and is associated with high morbidity and mortality. We aimed to analyze the effects of macitentan, selexipag, riociguat, and reoxygenation on HAPH, and to screen possible targets of these treatments for future drug screening. Rats were subjected to hypobaric hypoxia for 35 days to induce HAPH, and treated with vehicle or selexipag, macitentan, riociguat, or with reoxygenation, from days 21 to 35. Selexipag, macitentan, and reoxygenation prevented an increase in mean pulmonary artery pressure and hypoxia-induced right ventricular hypertrophy, compared to the vehicle. Riociguat had little effect. RNA-seq and proteomics revealed strong correlations between responses to the three drugs, which had almost identical effects. GO-enrichment revealed that the differentially expressed genes included those involved in metabolic regulation, transcription, and translation. Various molecular pathways were annotated. Selexipag, macitentan, and reoxygenation ameliorated HAPH. Serpina1, Cryz, and Cmc1 were identified, multi-omics screening, as key genes involved in HAPH. These findings provide new insights into the targeted drug mechanisms in HAPH.
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http://dx.doi.org/10.3389/fmed.2021.742436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595261PMC
November 2021

Atrazine exposure and recovery alter the intestinal structure, bacterial composition and intestinal metabolites of male Pelophylax nigromaculatus.

Sci Total Environ 2021 Nov 16:151701. Epub 2021 Nov 16.

College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, Hunan, China.

The pesticide atrazine poses a potential threat to the health of frogs living in farmland areas. The exposure concentration in traditional pesticide experiments is usually constant, while pesticide pollution in actual water may fluctuate due to periodic or seasonal application. We examined the effects of different concentrations of atrazine (50, 100 and 500 μg/L) over a 14-day exposure and a 7-day recovery on intestinal histology, bacterial composition and intestinal metabolites of male Pelophylax nigromaculatus. HE staining revealed that after a 14-day atrazine exposure, the 100 μg/L and 500 μg/L groups showed obvious cysts and significantly decreased intestinal crypt depth and villus height. After a 7-day recovery, the damaged intestine in the 100 μg/L group was partially recovered, while in the 500 μg/L exposure group there was no improvement. 16S rRNA gene analysis of intestinal bacteria showed that 500 μg/L atrazine exposure significantly caused a persistent decrease in bacterial α diversity. Compared to the control and other atrazine exposure groups, the 500 μg/L group showed significant changes in the relative abundance of predominant bacteria. In addition, most dominant bacteria in the 500 μg/L recovery group showed significant differences with the 50 μg/L and 100 μg/L recovery groups. Nontargeted metabolomics profiling based on UPLC/MS analysis showed that atrazine exposure and recovery induced changes in the intestinal metabolic profile. The changes in metabolites were mainly related to purine/pyrimidine metabolism, glycine, serine and threonine metabolism, and arginine and proline metabolism. In general, these pathways were closely related to energy metabolism and amino acid metabolism. These results suggest that the short-term exposure to 500 μg/L atrazine causes persistent harm to intestinal health. This study is an important step toward a better understanding of the toxic effects of atrazine exposure and recovery in frog intestines.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151701DOI Listing
November 2021

Dynamic contrast-enhanced CEST MRI using a low molecular weight dextran.

NMR Biomed 2021 Nov 15:e4649. Epub 2021 Nov 15.

Department of Radiology, Johns Hopkins University, Baltimore, Maryland, USA.

Natural and synthetic sugars have great potential for developing highly biocompatible and translatable chemical exchange saturation transfer (CEST) MRI contrast agents. In this study, we aimed to develop the smallest clinically available form of dextran, Dex1 (molecular weight, MW ~ 1 kDa), as a new CEST agent. We first characterized the CEST properties of Dex1 in vitro at 11.7 T and showed that the Dex1 had a detectable CEST signal at ~1.2 ppm, attributed to hydroxyl protons. In vivo CEST MRI studies were then carried out on C57BL6 mice bearing orthotopic GL261 brain tumors (n = 5) using a Bruker BioSpec 11.7 T MRI scanner. Both steady-state full Z-spectral images and single offset (1.2 ppm) dynamic dextran-enhanced (DDE) images were acquired before and after the intravenous injection of Dex1 (2 g/kg). The steady-state Z-spectral analysis showed a significantly higher CEST contrast enhancement in the tumor than in contralateral brain (∆MTR  = 0.010 ± 0.006 versus 0.002 ± 0.008, P = 0.0069) at 20 min after the injection of Dex1. Pharmacokinetic analyses of DDE were performed using the area under the curve (AUC) in the first 10 min after Dex1 injection, revealing a significantly higher uptake of Dex1 in the tumor than in brain tissue for tumor-bearing mice (AUC[0-10 min] = 21.9 ± 4.2 versus 5.3 ± 6.4%·min, P = 0.0294). In contrast, no Dex1 uptake was foundling in the brains of non-tumor-bearing mice (AUC[0-10 min] = -1.59 ± 2.43%·min). Importantly, the CEST MRI findings were consistent with the measurements obtained using DCE MRI and fluorescence microscopy, demonstrating the potential of Dex1 as a highly translatable CEST MRI contrast agent for assessing tumor hemodynamics.
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http://dx.doi.org/10.1002/nbm.4649DOI Listing
November 2021

A theoretical study on the photodegradation mechanism of the endocrine disrupting chemical p-nonylphenol induced by OH in water.

Mar Pollut Bull 2021 Nov 4;173(Pt B):113107. Epub 2021 Nov 4.

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China.

Nonylphenol (NP) has attracted significant attention because of its widespread environmental presence, toxicity, and pseudo-estrogen properties. Photolysis is the main degradation pathway of many organic pollutants in water. In this study, the photodegradation mechanism of p-nonylphenol (p-NP) induced by OH in water was investigated using the density functional theory (DFT). Based on the calculations of charges using natural population analysis (NPA), the reaction of OH with NP included the carbon addition of a benzene ring and the hydrogen extraction of a phenolic hydroxyl group. The structures of all reactants, products, and intermediates were optimized at the B3LYP/6-31+G(d,p) level. Using vibrational frequency and intrinsic reaction coordinate analyses, the transition state and its reaction pathway were confirmed. Additionally, the solvent effect results showed the reaction preferably took place in water. Our results provide a theoretical basis for understanding the transformative process and assessing the potential risk of NP in the environment.
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http://dx.doi.org/10.1016/j.marpolbul.2021.113107DOI Listing
November 2021

Synchronous conjugation of i-motif DNA and therapeutic siRNA on the vertexes of tetrahedral DNA nanocages for efficient gene silence.

Acta Pharm Sin B 2021 Oct 12;11(10):3286-3296. Epub 2021 Feb 12.

Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing 210009, China.

The functionality of DNA biomacromolecules has been widely excavated, as therapeutic drugs, carriers, and functionalized modification derivatives. In this study, we developed a series of DNA tetrahedron nanocages (Td), synchronous conjugating different numbers of i-(X) and therapeutic siRNA on four vertexes of tetrahedral DNA nanocage ([email protected], + = 4). This i-motif-conjugated Td exhibited good endosomal escape behaviours in A549 tumor cells, and the escape efficiency was affected by the number of i-motif. Furthermore, the downregulating mRNA and protein expression level of epidermal growth factor receptor (EGFR) caused by this siRNA embedded Td were verified in A549 cells. The tumor growth inhibition efficiency of the [email protected] treated group in tumor-bearing mice was significantly higher than that of non-i-motif-conjugated [email protected] (3.14-fold) and free siRNA (3.63-fold). These results demonstrate a general strategy for endowing DNA nanostructures with endosomal escape behaviours to achieve effective gene delivery and therapy.
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http://dx.doi.org/10.1016/j.apsb.2021.02.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546665PMC
October 2021

New way, new recommendation: Individualized treatment of novel antidiabetic drugs for people living with type 2 diabetes based on the cardiorenal risks.

Authors:
Xiang Xu Yan Bi

J Evid Based Med 2021 Nov 1. Epub 2021 Nov 1.

Department of Endocrinology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

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http://dx.doi.org/10.1111/jebm.12452DOI Listing
November 2021

Fraxinellone ameliorates intracerebral hemorrhage-induced secondary brain injury by regulating Krüppel-like transcription factor 2 expression in rats.

Brain Res Bull 2021 Dec 28;177:340-351. Epub 2021 Oct 28.

Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, Jiangsu Province, China.

Damage to the blood-brain barrier (BBB) is an important factor leading to intracerebral hemorrhage (ICH)-induced secondary brain injury (SBI). Krüppel-like transcription factor 2 (KLF2) plays an important role in the maintenance of the BBB. This study aims to detect the changes of KLF2 after ICH and evaluate the potential effects of fraxinellone on ICH-induced SBI and its correlation with KLF2. An ICH model was established by injecting autologous blood into the right basal ganglia of Sprague-Dawley (SD) rats. First, after ICH induction, the protein levels of KLF2 were reduced. Then, we found that the decrease of KLF2 protein levels induced by ICH could be effectively reversed with the treatment of fraxinellone in vascular endothelial cells. Furthermore, fraxinellone treatment effectively alleviated brain edema, decreased the levels of TNF-α and IL-1β, and improved neuronal cell degeneration induced by ICH. Meanwhile, fraxinellone ameliorated neurobehavioral disorders, motor and sensory impairments, and neurobehavioral disorders and memory loss caused by ICH. Collectively, these findings reveal that KLF2 may be a potential target for fraxinellone to exert neuroprotective effects after ICH, and fraxinellone could be a potential therapeutic agent for SBI after ICH.
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http://dx.doi.org/10.1016/j.brainresbull.2021.10.018DOI Listing
December 2021

Oncolytic Vaccinia Virus Harboring Lectin Inhibits the Growth of Cervical Cancer Cells Hela S3.

Mar Drugs 2021 Sep 23;19(10). Epub 2021 Sep 23.

College of Life Sciences and Medicine, Zhejiang Sci-Tch University, Hangzhou 310018, China.

lectin (AVL) is a C-type marine lectin produced by sponges. Our previous study demonstrated that genes encoding AVL enhanced the cytotoxic effect of oncolytic vaccinia virus (oncoVV) in a variety of cancer cells. In this study, the inhibitory effect of oncoVV-AVL on Hela S3 cervical cancer cells, a cell line with spheroidizing ability, was explored. The results showed that oncoVV-AVL could inhibit Hela S3 cells growth both in vivo and in vitro. Further investigation revealed that AVL increased the virus replication, promote the expression of OASL protein and stimulated the activation of Raf in Hela S3 cells. This study may provide insight into a novel way for the utilization of lection AVL.
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http://dx.doi.org/10.3390/md19100532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537278PMC
September 2021

Evidence and assessment of parenchymal patterns of ultrasonography for breast cancer detection among Chinese women: a cross-sectional study.

BMC Med Imaging 2021 Oct 19;21(1):152. Epub 2021 Oct 19.

Department of Ultrasound, First Affiliated Hospital of Fujian Medical University, No 20 Cha zhong Road, Taijiang District, Fuzhou, 350000, Fujian, China.

Background: Screening of breast cancer in asymptomatic women is important to evaluate for early diagnosis. In China ultrasound is a more frequently used method than mammography for the detection of breast cancer. The objectives of the study were to provide evidence and assessment of parenchymal patterns of ultrasonography for breast cancer detection among Chinese women.

Methods: Breast ultrasound examinations including the parenchymatous pattern of cytopathological confirmed breast cancer (n = 541) and age-matched cytopathological not confirmed breast cancer (n = 849) women were retrospectively reviewed by seven sonographer physicians. According to compositions of ducts, the thickness of the breast, diameter of ducts, fat lobules, and fibro glandular tissues, the breast parenchymatous pattern was categorized into heterogeneous (high percentage of fatty tissues), ductal (the inner diameters of ducts > 50% of the thick mass of the breast), mixed (the inner diameters of ducts was 50% of the thick mass of the breast), and fibrous categories (a dense classification of the breast).

Results: Heterogeneous (p < 0.0001, OR = 3.972) and fibrous categories (p < 0.0001, OR = 2.702) were higher among women who have cytopathological confirmed breast cancer than those who have not cytopathological confirmed breast cancer. The heterogeneous category was high-risk ultrasonographic examination category followed by the fibrous category. Agreements between sonographer physicians for categories of ultrasonic examinations were fair to good (Cohen's k = 0.591).

Conclusions: Breast cancer risk in Chinese asymptomatic women differ according to the ultrasonographic breast parenchymal pattern.

Level Of Evidence: III. Technical efficacy stage: 2.
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http://dx.doi.org/10.1186/s12880-021-00687-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527662PMC
October 2021

Influencing Factors and Cumulative Risk Analysis of Cervical Lymph Node Metastasis of Papillary Thyroid Microcarcinoma.

Front Oncol 2021 30;11:644645. Epub 2021 Sep 30.

Department of Endocrine and Metabolic Diseases, The Affiliated Hospital of Qingdao University, Qingdao, China.

Objective: To explore the influencing factors and cumulative risk of lymph node metastasis (LNM) in papillary thyroid microcarcinoma (PTMC) patients.

Methods: 607 patients confirmed PTMC pathologically after thyroidectomy were enrolled in this retrospective study. The rate of LNM was calculated. Different clinicopathological characteristics were compared in PTMC patients with and without LNM and in different subgroups of LNM, respectively. Correlation between clinicopathological characteristics and LNM was analyzed and the cumulative risk of LNM according to different clinicopathological characteristics was calculated.

Results: (1) There were 228 cases (37.56%) of PTMC combined with LNM. Compared with the non-lymph node metastasis group, the proportion of age <55 years, male, multiple foci, bilateral foci, diameter>0.5cm, extracapsular invasion, HT and intermediate-to-high risk stratification for recurrence of the LNM group was significantly increased (all <0.05);(2) Multivariate logistic regression analysis showed that age <55years, male, multiple foci, diameter>0.5cm,HT were independently positively correlated with LNM (all 0.05); (3) Subgroup analysis showed that women aged <55 years combined with HT and aged≥55 years combined with BMI≥25 kg/m were independently positively associated with LNM; (4) With the increase of the tumor diameter, the cumulative risk of LNM in group of age <55 years, males, and multiple foci increased gradually, and was higher than those of age≥55 years, females and single foci, respectively. (5) Among the 228 cases of LNM, the proportion of lymph nodes (LN) >5 and the positive rate of LN were both higher in male group than that in the female group. The proportion of multiple foci and HT in LLNM group was higher than that in CLNM group (all P<0.05).

Conclusion: Age <55 years, males, multiple foci, diameter >0.5cm and HT were independent risk factors of LNM; HT was an independent risk factor for LNM in female <55 years old, and BMI≥25 kg/m was an independent risk factor for LNM in female ≥55 years old; The increase of tumor diameter in age <55 years, males, multiple foci, and bilateral foci increased the cumulative risk of LNM, respectively; The number of LNM and the positive rate of LNM were both higher in male, and patients with multiple foci or HT were more likely to develop into LLNM.
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http://dx.doi.org/10.3389/fonc.2021.644645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514816PMC
September 2021

FAK-targeting PROTAC demonstrates enhanced antitumor activity against KRAS mutant non-small cell lung cancer.

Exp Cell Res 2021 11 12;408(2):112868. Epub 2021 Oct 12.

Department of Cardiothoracic Surgery, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. Electronic address:

Focal adhesion kinase (FAK) has been established as a promising therapeutic target for KRAS mutant non-small cell lung cancer (NSCLC). However, phase II clinical trials of a FAK inhibitor (Defactinib) have only shown modest antitumor activity. To address this challenge, here we report the use of a FAK-targeting proteolysis targeting chimera (D-PROTAC) to treat KRAS mutant NSCLC. We validated that D-PROTAC could efficiently eliminate FAK protein via the ubiquitin-proteasome pathway in KRAS mutant NSCLC A427 cells, causing over 90% degradation at 800 nM. After comparing both in vitro and in vivo therapeutic efficacies, we demonstrated that D-PRTOAC outperformed Defactinib in inhibiting tumor growth. Specifically, D-PROTAC at 800 nM reduced cell viability, migration, and invasion by ∼80%. Furthermore, a ∼85% suppression of tumor growth was elicited by D-PROTAC when intratumorally administrated at 10 mg/kg in subcutaneous A427-bearing mice. These results thus demonstrate for the first time that PROTACs may serve as promising therapeutic agents for the intractable NSCLC harboring KRAS mutations.
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http://dx.doi.org/10.1016/j.yexcr.2021.112868DOI Listing
November 2021

heterozygosity ameliorates chronic hypoxia-induced pulmonary hypertension and right ventricular hypertrophy in male rats.

Clin Exp Hypertens 2021 Oct 14:1-11. Epub 2021 Oct 14.

Laboratory of Translational Medicine, Medical Innovation Research Division of Chinese Pla General Hospital, Beijing, China.

Background: Pulmonary hypertension (PH) is a rare and deadly disease characterized by remodeling of the pulmonary vasculature and increased pulmonary artery pressure. hypobaric pulmonary hypertension (HPH) is clinically classified as group 4 of pulmonary hypertension and has a poor prognosis . Previous reports showed that HPH was associated with increased endoplasmic reticulum (ER) stress. The protein kinase R-like endoplasmic reticulum kinase (PERK) is an ER-associated stress protein. However, to date, its physiological effects on HPH and RVF development remains unknown. This study aimed to assess PERK's role in HPH and RV function using experimental model.

Methods: -knockout male Sprague-Dawley rats were generated and were housed in either a hypobaric chamber or in a normoxic environment. After stimulation for 4 weeks, the hemodynamic parameters of the rats were measured. The heart and lungs were harvested for pathological observation. Blood was collected for the detection of inflammatory indexes. The right ventricle tissue was collected to assess phosphorylated-AKT, ROCK1, ET1, and MMP2 protein expression.

Results: We Firstly Generated Perk+/− Rats,: Under normal conditions, rats showed no changes in mPAP(mean pulmonary artery pressure), RVHI(Right ventricular hypertrophy index), cardiomyocyte size and interstitial fibrosis, and pulmonary vascular remodeling. However, in response to chronic hypoxia, rats exhibited decreased in mPAP, RVHI, ventricular fibrosis, and lung remodeling compared to wild-type rats. rats also showed lower expression of phosphor-AKT, ROCK1, ET1, and MMP2 protein in response to chronic hypoxia.

Conclusions: These findings suggest that heterozygosity protects against HPH and may be a suitable target for treating HPH.
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http://dx.doi.org/10.1080/10641963.2021.1984501DOI Listing
October 2021

New role of sertraline against Toxoplasma gondii-induced depression-like behaviours in mice.

Parasite Immunol 2021 Dec 20;43(12):e12893. Epub 2021 Oct 20.

Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian University, Yanji, Jilin, China.

Toxoplasma gondii (T. gondii) is a neurotropic protozoan parasite, which can cause mental and behavioural disorders. The present study aimed to elucidate the effects and underlying molecular mechanisms of sertraline (SERT) on T. gondii-induced depression-like behaviours. In the present study, a mouse model and a microglial cell line (BV2 cells) model were established by infecting with the T. gondii RH strain. In in vivo and in vitro experiments, the underlying molecular mechanisms of SERT in inhibiting depression-like behaviours and cellular perturbations caused by T. gondii infection were investigated in the mouse brain and BV2 cells. The administration of SERT significantly ameliorated depression-like behaviours in T. gondii-infected mice. Furthermore, SERT inhibited T. gondii proliferation. Treatment with SERT significantly inhibited the activation of microglia and decreased levels of pro-inflammatory cytokines such as tumour necrosis factor-alpha, and interferon-gamma, by down-regulating tumour necrosis factor receptor 1/nuclear factor-kappa B signalling pathway, thereby ameliorating the depression-like behaviours induced by T. gondii infection. Our study provides insight into the underlying molecular mechanisms of the newly discovered role of SERT against T. gondii-induced depression-like behaviours.
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http://dx.doi.org/10.1111/pim.12893DOI Listing
December 2021

Leader-Following Consensus of Heterogeneous Linear Multiagent Systems With Communication Time-Delays via Adaptive Distributed Observers.

IEEE Trans Cybern 2021 Oct 12;PP. Epub 2021 Oct 12.

This article investigates the leader-following consensus problem of heterogeneous linear multiagent systems under switching and directed topologies. It is assumed that the communication between agents suffers from time-varying delays and only the neighboring agents of the leader are able to get access to the information of the leader agent, including its agent matrices. A key technical lemma on the input to state stability of time-delayed systems is first established with which the main results of this article can be obtained. An adaptive distributed observer, taking into consideration of communication time delays, is proposed for each follower to estimate the leader's system matrices and its state. Then, a distributed controller based on this adaptive observer is developed. We show that the resulting closed-loop multiagent system achieves the leader-following output consensus. Two examples are finally given to illustrate the effectiveness of the proposed controller.
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http://dx.doi.org/10.1109/TCYB.2021.3115124DOI Listing
October 2021

A five-year prospective evaluation of anticholinergic cognitive burden and falls in the Malaysian elders longitudinal research (MELoR) study.

Arch Gerontol Geriatr 2021 Sep 30;98:104535. Epub 2021 Sep 30.

Ageing and Age-Associated Disorders Research Group, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Sciences, Faculty of Healthcare and Medical Sciences, Sunway University, Bandar Sunway, Selangor. Electronic address:

Background: While anticholinergic use is associated with stroke, dementia and mortality, few have evaluated its potential link with falls. To determine the relationship between anticholinergic cognitive burden (ACB) and falls over five years using the Malaysian Elders Longitudinal Research (MELoR).

Methods: Community-dwelling adults aged 55 years and over were recruited through electoral roll sampling. Data obtained at baseline and follow-up (FU) at two and five years were included. Falls in the preceding 12 months were recorded.

Results: Of the 1499 individuals (mean (SD) age= 68.9(7.5) yrs and 53.3% female) with information on baseline ACB exposure, 575(38.4%) had ACB scores of 1-2 and 117(7.8%) had ACB scores ≥3. Differences in age, ethnicity, smoking status, diabetes, hypertension, cardiovascular disease, arthritis and education existed between ACB groups. Fall occurrence differed between ACB groups at recruitment (p = 0.004) and 2-year FU (p = 0.001) but not at 5-year FU (p = 0.053). Logistic regression revealed an independent association between ACB 1-2 and falls at baseline (odds ratio, OR (95% confidence interval, CI) =1.412(1.035-1.926)) and ACB≥3 and falls at 2-yr FU (OR (95%CI) =2.098(1.032-4.263)) following adjustment for confounders.

Conclusion: Low level exposure to drugs with anticholinergic properties was associated cross-sectionally with falls, while exposure to higher levels were prospectively associated with falls at 2-year but not at 5-year FU. Future studies should determine whether avoidance of drugs with anticholinergic effects will lead to reduction in falls.
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http://dx.doi.org/10.1016/j.archger.2021.104535DOI Listing
September 2021

Alterations in Peripheral B Cell Subsets Correlate with the Disease Severity of Human Glaucoma.

J Inflamm Res 2021 21;14:4827-4838. Epub 2021 Sep 21.

Clinical Immunology Translational Medicine Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.

Background: Glaucoma is a group of retinal neurodegenerative diseases causing irreversible visual impairment. The pathogenesis of this disease is complicated. Studies have shown that the immune system is involved in the neurodegenerative process of glaucoma. There are continuous evidences that autoantibodies play a crucial role in the pathogenesis of glaucoma. However, focuses on B cells, the antibody-producing cells in glaucoma are surprisingly limited.

Methods: Fresh peripheral blood samples were collected from 44 glaucoma patients (38 with primary angle-closure glaucoma (PACG) and 6 with (primary open-angle glaucoma POAG)) and 36 age-matched healthy donors (HD). Density gradient centrifugation was performed to obtain peripheral blood mononuclear cells (PBMC). Flow cytometry was performed to determine B cell phenotypes. The severity of glaucoma was determined based on the mean deviation (MD) of visual field.

Results: In this study, we demonstrated that total B cells was significantly increased in glaucoma patients compared to HD. Next, we checked changes of different B cell subsets in glaucoma. Glaucoma patients were found to have a significant increase in the frequencies of antibody-secreting cells (ASC)/plasmablasts, naïve, and CD19 CD27 IgD double negative (DN) subpopulations, but a decrease in the CD27 IgD unswitched memory compartment. Notably, we found that the increment of CD27 IgD DN B cells was significantly magnified according to the clinical severity.

Conclusion: We demonstrate, for the first time, that peripheral B cell subsets are altered and unveil the correlation of a newly identified pro-inflammatory CD27 IgD DN subset with clinical features of glaucoma, suggesting that these B cell subsets could serve as potential biomarkers to monitor the disease progression of glaucoma patients.
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http://dx.doi.org/10.2147/JIR.S329084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464325PMC
September 2021

Investigation of a complete squeeze-film damping model for MEMS devices.

Microsyst Nanoeng 2021 22;7:54. Epub 2021 Jul 22.

Ningbo Institute of Northwestern Polytechnical University, Frontiers Science Center for Flexible Electronics (FSCFE), MIIT Key Laboratory of Flexible Electronics (KLoFE), Shaanxi Key Laboratory of Flexible Electronics (KLoFE), Institute of Flexible Electronics (IFE), Northwestern Polytechnical University, 710072 Xi'an, Shaanxi China.

Dynamic performance has long been critical for micro-electro-mechanical system (MEMS) devices and is significantly affected by damping. Different structural vibration conditions lead to different damping effects, including border and amplitude effects, which represent the effect of gas flowing around a complicated boundary of a moving plate and the effect of a large vibration amplitude, respectively. Conventional models still lack a complete understanding of damping and cannot offer a reasonably good estimate of the damping coefficient for a case with both effects. Expensive efforts have been undertaken to consider these two effects, yet a complete model has remained elusive. This paper investigates the dynamic performance of vibrated structures via theoretical and numerical methods simultaneously, establishing a complete model in consideration of both effects in which the analytical expression is given, and demonstrates a deviation of at least threefold lower than current studies by simulation and experimental results. This complete model is proven to successfully characterize the squeeze-film damping and dynamic performance of oscillators under comprehensive conditions. Moreover, a series of simulation models with different dimensions and vibration statuses are introduced to obtain a quick-calculating factor of the damping coefficient, thus offering a previously unattainable damping design guide for MEMS devices.
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http://dx.doi.org/10.1038/s41378-021-00279-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433295PMC
July 2021

Kynurenine derivative 3-HAA is an agonist ligand for transcription factor YY1.

J Hematol Oncol 2021 09 25;14(1):153. Epub 2021 Sep 25.

Basic Medical Institute, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The 3-hydroxyanthranilic acid (3-HAA), a derivative of kynurenine, was reported to suppress tumor growth. However, the function of 3-HAA largely remains unclear. Here, we report that 3-hydroxyanthranilic acid (3-HAA) is lower in tumor cells, while adding exogenous 3-HAA induces apoptosis in hepatocellular carcinoma by binding YY1. This 3-HAA binding of YY1 leads to phosphorylation of YY1 at the Thr 398 by PKCζ, concomitantly enhances YY1 chromatin binding activity to increase expression of target genes. These findings demonstrate that 3-HAA is a ligand of YY1, suggesting it is a promising therapeutic candidate for HCC.
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http://dx.doi.org/10.1186/s13045-021-01165-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465765PMC
September 2021

Macrophages mediated delivery of chlorin e6 and treatment of lung cancer by photodynamic reprogramming.

Int Immunopharmacol 2021 Nov 22;100:108164. Epub 2021 Sep 22.

Department of respiratory, Taihe Hospital of Shiyan, Hubei University of Medicine, Renmin road No. 30, Shiyan, Hubei 442000, China; Department of Pharmacology, School of Basic Medical Sciences, Hubei University of Medicine, Renmin road No. 30, Shiyan, Hubei 442000, China; Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, Renmin road No. 30, Shiyan, Hubei 442000, China. Electronic address:

Photodynamic therapy (PDT) is an emerging anti-tumor strategy.Photosensitizer chlorin e6 (Ce6) can induce photodynamic effect to selectively damage lung cancer cells.In order to further improve its tumor targeting ability, macrophages can be applied as carrier to deliver Ce6 to lung cancer.Tumor associated macrophages (TAM) are important immunocytes in lung cancer immune microenvironment. TAM play crucial role in tumor promotion due to the Immunosuppressive property, reprogramming phenotype of TAM therefore has become a promising strategy.Based on this, in the present study, we suppose that TAM can be used as carrier to deliver Ce6 to lung cancer and be reprogrammed to M1 phenotype by photodynamic action to mediate anti-lung cancer efficacy.The results showed TAM could load with Ce6 and keep viability in the absence of near infrared irradiation (NIR).Moreover, Its viability decreased little within 10 h after NIR.Ce6-loaded TAM could deliver Ce6 to lung cancer cells and retain some drugs in TAM per se.After NIR, phagocytosis of macrophages was enhanced. The expressions of GBP5, iNOS and MHC-II was up-regulated, which indicated TAM were polarized to M1 phenotype.Finally, the study also found the reprogrammed macrophages could inhibit the proliferation and promote the apoptosis of lung cancer cells.These results suggested that macrophages could deliver Ce6 to lung cancer and exhibit anti-lung cancer effect through photodynamic reprogramming.This study provides a novel approach for combining photodynamic action with anti-tumor immunotherapy.
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http://dx.doi.org/10.1016/j.intimp.2021.108164DOI Listing
November 2021

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

Cell Res 2021 Sep 24. Epub 2021 Sep 24.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.

The AMP-activated protein kinase (AMPK) is a central regulator of energy homeostasis. Although much has been learned on how low energy status and glucose starvation activate AMPK, how AMPK activity is properly controlled in vivo is still poorly understood. Here we report that UHRF1, an epigenetic regulator highly expressed in proliferating and cancer cells, interacts with AMPK and serves to suppress AMPK activity under both basal and stressed conditions. As a nuclear protein, UHRF1 promotes AMPK nuclear retention and strongly suppresses nuclear AMPK activity toward substrates H2B and EZH2. Importantly, we demonstrate that UHRF1 also robustly inhibits AMPK activity in the cytoplasm compartment, most likely as a consequence of AMPK nucleocytoplasmic shuttling. Mechanistically, we found that UHRF1 has no obvious effect on AMPK activation by upstream kinases LKB1 and CAMKK2 but inhibits AMPK activity by acting as a bridging factor targeting phosphatase PP2A to dephosphorylate AMPK. Hepatic overexpression of UHRF1 showed profound effects on glucose and lipid metabolism in wild-type mice but not in those with the liver-specific knockout of AMPKα1/α2, whereas knockdown of UHRF1 in adipose tissue led to AMPK activation and reduced sizes of adipocytes and lipogenic activity, highlighting the physiological significance of this regulation in glucose and lipid metabolism. Thus, our study identifies UHRF1 as a novel AMPK gate-keeper with critical roles in cellular metabolism.
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http://dx.doi.org/10.1038/s41422-021-00565-yDOI Listing
September 2021

Correction to: Serum IFN-γ levels predict the therapeutic effect of mesenchymal stem cell transplantation in active rheumatoid arthritis.

J Transl Med 2021 Sep 23;19(1):402. Epub 2021 Sep 23.

First Department, State Key Laboratory of Trauma, Burn and Combined Injury, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing, 400042, People's Republic of China.

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http://dx.doi.org/10.1186/s12967-021-02917-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461920PMC
September 2021

The Flavonoid Kurarinone Regulates Macrophage Functions via Aryl Hydrocarbon Receptor and Alleviates Intestinal Inflammation in Irritable Bowel Syndrome.

J Inflamm Res 2021 3;14:4347-4359. Epub 2021 Sep 3.

Clinical Immunology Translational Medicine Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.

Background: Irritable bowel syndrome (IBS) is characterized with abdominal pain, bloating, and changes in bowel habits, and dealing with IBS is still a clinical challenge. The pathogenesis of IBS has been reported to be linked to low-grade mucosal inflammation, and macrophages contribute to the pathological process of this disease. Kurarinone (KAR), a flavanoid derived from , has been found medically effective in many inflammatory conditions and cancers. KAR was previously reported to inhibit LPS-induced expression of inflammatory cytokines in macrophages, whether and how KAR regulates the functions of macrophage in IBS remains to be elusive.

Methods: We established a TNBS-induced IBS mouse model, in which KAR was administrated, and mucosal cytokine expression was measured by qRT-PCR. Additionally, mouse macrophages were generated in vitro and their responses to LPS were evaluated by flow cytometry and qRT-PCR. AhR or AhR macrophages were transferred into DTx-treated CD11b-DTR transgenic mice to investigate the role of AhR in IBS. We collected colonic biopsies and peripheral blood samples from 64 patients with IBS, and analyzed AhR expression by qRT-PCR.

Results: We found KAR effectively alleviated visceral hypersensitivity and maintained intestinal barrier functions in mice with IBS. KAR inhibited LPS-induced macrophage activation and expression of pro-inflammatory genes, while increased anti-inflammatory gene expression including IL-10 in an AhR-dependent manner. Using macrophage-depleted mice, we found that chimera mice with AhR macrophages were more susceptible to TNBS-induced IBS and the therapeutic effect of KAR on IBS was significantly impaired in mice with AhR macrophages. Additionally, we found AhR expression in macrophages of IBS patients was associated with the disease severity.

Conclusion: Our findings provide new evidences that KAR regulates IBS development via macrophage-intrinsic AhR. KAR might show promise as an immunomodulatory therapeutic agent in treating IBS.
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http://dx.doi.org/10.2147/JIR.S329091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446718PMC
September 2021

Linking nuclear matrix-localized PIAS1 to chromatin SUMOylation via direct binding of histones H3 and H2A.Z.

J Biol Chem 2021 10 17;297(4):101200. Epub 2021 Sep 17.

Shanghai Key Laboratory of Regulatory Biology, Fengxian District Central Hospital-ECNU Joint Center of Translational Medicine, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China. Electronic address:

As a conserved posttranslational modification, SUMOylation has been shown to play important roles in chromatin-related biological processes including transcription. However, how the SUMOylation machinery associates with chromatin is not clear. Here, we present evidence that multiple SUMOylation machinery components, including SUMO E1 proteins SAE1 and SAE2 and the PIAS (protein inhibitor of activated STAT) family SUMO E3 ligases, are primarily associated with the nuclear matrix rather than with chromatin. We show using nuclease digestion that all PIAS family proteins maintain nuclear matrix association in the absence of chromatin. Of importance, we identify multiple histones including H3 and H2A.Z as directly interacting with PIAS1 and demonstrate that this interaction requires the PIAS1 SAP (SAF-A/B, Acinus, and PIAS) domain. We demonstrate that PIAS1 promotes SUMOylation of histones H3 and H2B in both a SAP domain- and an E3 ligase activity-dependent manner. Furthermore, we show that PIAS1 binds to heat shock-induced genes and represses their expression and that this function also requires the SAP domain. Altogether, our study reveals for the first time the nuclear matrix as the compartment most enriched in SUMO E1 and PIAS family E3 ligases. Our finding that PIAS1 interacts directly with histone proteins also suggests a molecular mechanism as to how nuclear matrix-associated PIAS1 is able to regulate transcription and other chromatin-related processes.
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http://dx.doi.org/10.1016/j.jbc.2021.101200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496182PMC
October 2021

Resveratrol modulates Toxoplasma gondii infection induced liver injury by intervening in the HMGB1/TLR4/NF-κB signaling pathway.

Eur J Pharmacol 2021 Nov 9;910:174497. Epub 2021 Sep 9.

Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Molecular Medicine Research Center, College of Pharmacy, Yanbian University, Yanji, 133002, Jilin, China; College of Integration Science, Yanbian University, Yanji, 133002, Jilin Province, China. Electronic address:

Toxoplasma gondii (T. gondii) is an obligate intracellular parasite that can cause liver diseases in the host, including hepatitis and hepatomegaly. High mobility group box 1 (HMGB1) is the main inflammatory mediator causing cell injury or necrosis. HMGB1 binds to toll like receptor 4 (TLR4), then activates the nuclear factor-κB (NF-κB) signaling pathway, which promotes the release of inflammatory factors. Our previous studies showed that HMGB1 mediated TLR4/NF-κB signaling pathway plays an important role in liver injury induced by T. gondii infection. Resveratrol (RSV) is a small polyphenol, which has anti-inflammatory, anti-cancer, anti-T. gondii effect. However, the effect of RSV on liver injury caused by T. gondii infection is unclear. This study used the RH strain tachyzoites of T. gondii to infect murine liver line, NCTC-1469 cells to establish an in vitro model and acute infection of mice for the in vivo model to explore the protective effect of RSV on liver injury induced by T. gondii infection. The results showed that RSV inhibited the proliferation of T. gondii in the liver, reduced the alanine aminotransferase/aspartate aminotransferase levels and pathological liver damage. Additionally, RSV inhibited the production of tumor necrosis factor-α, inducible nitric oxide synthase and HMGB1 by interfering with the TLR4/NF-κB signaling pathway. These results indicate that RSV can protect liver injury caused by T. gondii infection by intervening in the HMGB1/TLR4/NF-κB signaling pathway. This study will provide a theoretical basis for RSV treatment of T. gondii infection induced liver injury.
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http://dx.doi.org/10.1016/j.ejphar.2021.174497DOI Listing
November 2021

Role of hyperbaric oxygen in glioma: a narrative review.

Med Gas Res 2022 Jan-Mar;12(1):1-5

Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.

Gliomas are common brain mass with a high mortality rate. Patients with gliomas have a severely bad outcome, with an average survive duration less 15 months because of high recurrent rate and being resistant to radio-therapy and chemistry drugs therapy. Hyperbaric oxygen is extensively taken as an adjuvant treatment for various disease conditions. To know the characteristics of hyperbaric oxygen as a remedy for gliomas, we find that, in general, hyperbaric oxygen shows an obviously positive effect on the treatment of gliomas, and it can also relieve the complications caused by postoperative radiotherapy and chemotherapy of gliomas. Whereas, several researches have shown that hyperbaric oxygen promotes glioma progression.
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http://dx.doi.org/10.4103/2045-9912.324589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447952PMC
October 2021
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