Publications by authors named "Xiang Ma"

528 Publications

Effect of orlistat on live birth rate in overweight or obese women undergoing IVF-ET: A randomized clinical trial.

J Clin Endocrinol Metab 2021 May 15. Epub 2021 May 15.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University; The Key Laboratory of Reproductive Endocrinology of Ministry of Education; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, China.

Context: Obesity management prior to infertility treatment remains a challenge. To date, results from randomized clinical trials involving weight loss by lifestyle interventions have shown no evidence of improved live birth rate.

Objective: To determine whether pharmacologic weight-loss intervention before in vitro fertilization and embryo transfer (IVF-ET) can improve live birth rate among overweight or obese women.

Design, Setting, And Participants: We conducted a randomized, double-blinded, placebo-controlled trial across 19 reproductive medical centers in China, from July 2017 to January 2019. A total of 877 infertile women scheduled for IVF who had a body mass index of 25kg/m 2 or greater were randomly assigned.

Interventions: The participants were randomized to receive orlistat (n=439) or placebo (n=438) treatment for 4-12 weeks.

Main Outcomes And Measures: Live birth rate after fresh embryo transfer.

Results: The live birth rate was not significantly different between the two groups (112 of 439 [25.5%] with orlistat and 112 of 438 [25.6%] with placebo; P=.984). No significant differences existed between the groups as to the rates of conception, clinical pregnancy, and pregnancy loss. A statistically significant increase in singleton birthweight was observed after orlistat treatment (3487.50g versus 3285.17g in the placebo group; P=.039). The mean change in body weight during the intervention was -2.49kg in the orlistat group, as compared to -1.22kg in the placebo group, with a significant difference (P=.005).

Conclusions: Orlistat treatment, prior to IVF-ET, did not improve live birth rate among overweight or obese women, although it was beneficial for weight reduction.
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http://dx.doi.org/10.1210/clinem/dgab340DOI Listing
May 2021

miR-1301-3p Promotes Cell Proliferation and Facilitates Cell Cycle Progression Targeting SIRT1 in Gastric Cancer.

Front Oncol 2021 27;11:664242. Epub 2021 Apr 27.

Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

So far, many existing evidences indicate that microRNAs (miRNA) are closely associated with the tumorigenesis and progression of various tumors. It has been reported that miR-1301-3p is abnormally expressed in several malignant tumors. However, the role of miR-1301-3p in gastric cancer (GC) remains unclear and is worth studying. Through qRT-PCR, the expression of miR-1301-3p and SIRT1 were detected in GC tissues and cells. The cell proliferation and cell cycle were measured through CCK-8 assay and clone formation assay. Dual luciferase reporter assay was used to determine the target of miR-1301-3p. Though tumorigenesis assay, we monitored the effect of miR-1301-3p on GC cell growth . miR-1301-3p was upregulated in GC tissues and cells in our study. Overexpression of miR-1301-3p accelerated GC cell proliferation, cell cycle progression and tumorigenesis. Notably, altering the expression miR-1301-3p caused deregulation of Cyclin D1, CDK4, c-Myc and P21. Furthermore, SIRT1 was the direct target of miR-1301-3p by luciferase reporter assay. After transfecting with miR-1301-3p inhibitor, we found that knockdown of SIRT1 could enhance the ability of proliferation. Our results identify miR-1301-3p as a novel potential therapeutic target that is associated with the tumorigenesis and progression of gastric cancer.
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http://dx.doi.org/10.3389/fonc.2021.664242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112236PMC
April 2021

Controlled single-mode emission in quantum dot micro-lasers.

Opt Express 2021 Apr;29(9):13193-13203

In this paper, we demonstrate an efficient and easy fabrication method for whispering-gallery-mode (WGM) manipulation and report the first electrically driven single-mode quantum dot micro-ring (QDMR) lasers. Using self-assembled InAs/InAlGaAs QD active layers with deeply etched azimuthal gratings, continuous-wave (CW) lasing with controllable single-mode emission wavelengths covering 1300 nm to 1370 nm has been achieved. A record-high side-mode-suppression-ratio (SMSR) value of 49 dB is obtained. These QDMR lasers exhibit excellent single-mode lasing stabilities over the current and temperature tuning range with a thermal tunability of 0.092 nm/°C. The concept is applicable to other wavelength bands depending on the gain spectrum, demonstrating a feasible solution in realizing energy-efficient and densely integrated photonic building blocks.
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http://dx.doi.org/10.1364/OE.421037DOI Listing
April 2021

Engendering persistent organic room temperature phosphorescence by trace ingredient incorporation.

Sci Adv 2021 May 7;7(19). Epub 2021 May 7.

Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.

Pure organic persistent room temperature phosphorescence (RTP) has shown great potential in information encryption, optoelectronic devices, and bio-applications. However, trace impurities are generated in synthesis, causing unpredictable effects on the luminescence properties. Here, an impurity is isolated from a pure organic RTP system and structurally characterized that caused an unusual ultralong RTP in matrix even at 0.01 mole percent content. Inspired by this effect, a series of compounds are screened out to form the bicomponent RTP system by the trace ingredient incorporation method. The RTP quantum yields reach as high as 74.2%, and the lifetimes reach up to 430 ms. Flexible application of trace ingredients to construct RTP materials has become an eye-catching strategy with high efficiency, economy, and potential for applications as well as easy preparation.
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http://dx.doi.org/10.1126/sciadv.abf9668DOI Listing
May 2021

MicroRNA-653-5p Promotes Gastric Cancer Proliferation and Metastasis by Targeting the SOCS6-STAT3 Pathway.

Front Mol Biosci 2021 15;8:655580. Epub 2021 Apr 15.

Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

MicroRNAs (miRNAs) are emerging as significant regulators of the tumorigenesis of gastric cancer (GC), and may be effective biomarkers for diagnosis, prognosis, and therapeutic targeting for GC. In this study, miR-653-5p was found to be significantly upregulated in GC tissues, serum, and cell lines and was strongly associated with poor prognosis in patients with GC. Furthermore, miR-653-5p promoted GC cell proliferation and metastasis and . Suppressor of cytokine signaling 6 (SOCS6) was directly targeted by miR-653-5p, and SOCS6 attenuated miR-653-5p-mediated GC cell growth, migration, and invasion. In addition, SOCS6-mediated inactivation of the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway was also reversed by the administration of miR-653-5p. The findings from this study support a novel regulatory axis between miR-653-5p, SOCS6, and JAK2/STAT3 that may be a target for diagnosis and therapeutic intervention for GC.
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http://dx.doi.org/10.3389/fmolb.2021.655580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082248PMC
April 2021

[Cathepsin-B involved in effect of electroacupuncture by inhibiting the activation of NLRP3 inflammasome in rats with acute gouty arthritis].

Zhen Ci Yan Jiu 2021 Apr;46(4):295-300

College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016,China.

Objective: To observe the effect of electroacupuncture (EA) on Cathepsin-B in the synovium of the knee joint of acute gouty arthritis(AGA) rats, so as to explore the mechanism of EA in the treatment of AGA.

Methods: A total of 60 male Wistar rats were randomly divided into normal control,model, medication and EA groups, with 15 rats in each group. Rat model of AGA was established by injection of 0.2 mL sodium urate crystal suspension into the left knee joint cavity. The rats in the medication group were treated with colchicine by gavage(0.3 mg·kg·d), and the rats in the EA group were treated with EA at the left "Sanyinjiao" (SP6) and "Zusanli"(ST36) for 10 min each time, once a day for a week. The Coderre gait grading standard was used to score the gait of rats. The pathological morphology of synovial tissue of the left knee joint was observed by H.E. staining. The expression levels of Cathepsin-B protein and Nod-like receptor pyrin domain 3(NLRP3), apoptosis-associated speck-like protein containing CARD (ASC),Caspase-1, interleukin-1β(IL-1β) and IL-18 mRNAs were detected by Western blot and real-time fluorescence quantitative PCR, respectively.

Results: Compared with the normal control group, the degree of synovitis infiltration in the model group was more serious. And the gait score,the protein expression level of Cathepsin-B and the mRNA expression levels of NLRP3,ASC,Caspase-1, IL-1β,IL-18 were significantly increased (<0.01).After the interventions, the degree of inflammatory infiltration was mild, The gait score, the protein expression level of Cathepsin-B and the mRNA expression levels of NLRP3 and ASC,Caspase-1,IL-1β,IL-18 were significantly decreased in both medication and EA groups in contrast to the model group (<0.01, <0.05). Compared with medication group, the mRNA expression levels of Caspase-1 and IL-18 in the EA group were increased (<0.05).

Conclusion: EA may inhibit the activation of NLRP3 inflammasome by reducing the activity of Cathepsin-B in the synovium of the knee joint, so as to treat AGA.
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http://dx.doi.org/10.13702/j.1000-0607.200240DOI Listing
April 2021

Correlations of SNHG5 genetic polymorphisms with susceptibility and prognosis to gastric cancer in a Chinese population.

Genomics 2021 Apr 15;113(4):1754-1760. Epub 2021 Apr 15.

Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China; Department of General Surgery, Liyang People's Hospital, Liyang Branch Hospital of Jiangsu Province Hospital, Liyang, Jiangsu Province, China. Electronic address:

The most studied genetic polymorphisms associated with gastric cancer (GC) risk are located in protein-coding genes. However, these sited in long noncoding RNA (lncRNA) are not adequately explored yet. Here, we designed a case-control study of 848 cases and 880 controls to investigate the associations of polymorphisms (rs61396151, rs1059307, rs11961028, rs9351065) in lncRNA SNHG5 with the risk and prognosis of GC. The results indicate rs61396151 associated with decreased risk of GC (OR = 0.78, 95% CI = 0.62-0.96), but there were no correlations observed with the clinicopathological features of GC (P > 0.05). However, the CA genotype of rs61396151 was correlated with poor overall survival rate in a multivariate cox regression model (HR = 1.91, P = 0.040), but it was reversed with adjustment for age, gender and TNM stage (HR = 1.35, P = 0.213). Collectively, our results highlight the importance of SNHG5-related polymorphisms to GC susceptibility and prognosis.
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http://dx.doi.org/10.1016/j.ygeno.2021.04.025DOI Listing
April 2021

Genetic polymorphism of IDOL gene was associated with the susceptibility of coronary artery disease in Han population in Xinjiang, China.

Hereditas 2021 Apr 12;158(1):12. Epub 2021 Apr 12.

Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, P.R. China.

Background: Coronary artery disease (CAD) is the leading cause of death worldwide. In this study, we aimed to explore whether some genetic variants of the human IDOL gene were associated with CAD among Chinese population in Xinjiang.

Methods: We designed two independent case-control studies. The first one included in the Han population (448 CAD patients and 343 controls), and the second one is the Uygur population (304 CAD patients and 318 controls). We genotyped three SNPs (rs2072783, rs2205796, and rs909562) of the IDOL gene.

Results: Our results revealed that, in the Han female subjects, for rs2205796, the distribution of alleles, dominant model (TT vs. GG + GT) and the additive model (GG + TT vs. GT) showed significant differences between CAD patients and the control subjects (P = 0.048, P = 0.014, and P = 0.032, respectively).

Conclusions: The rs2205796 polymorphism of the IDOL gene is associated with CAD in the Chinese Han female population in Xinjiang, China.
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http://dx.doi.org/10.1186/s41065-021-00178-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042894PMC
April 2021

First Report of Grammothele lineata Causing Stem Rot of Areca catechu in Hainan Province, China.

Plant Dis 2021 Apr 8. Epub 2021 Apr 8.

Haikou, China;

Areca catechu L. (areca) belongs to the Arecaceae family, which is composed of 181 genera and 2,600 species (Christenhusz and Byng 2016), is cultivated extensively in Southern and Southeastern Asia (Peng et al. 2015). Areca has a long history for its important economic and medicinal benefits and is one of the most important commercial crops in Hainan province, China. In recent years, root rot and stem rot diseases have occurred, causing areca plants to wither and even die. The serious symptoms mainly appeared in the Hainan province (Li et al. 2006). In March 2018, the rotten tissues of the diseased plants were observed to become brittle, brown, and even black from the stem base to the root; the outer leaves turned yellow, dry, and dropping in areca plantations of Qionghai county. The disease can spread from individual plants to the whole plantation in one to two years, with the characteristics of large-scale occurrence and rapid transmission, causing huge economic losses. Diseased tissues (5 × 5 mm) were disinfected with 75% ethanol for 30 s, 1% HgCl2 for 1 min, washed in sterile water, placed on potato dextrose agar (PDA) medium and incubated at 28°C (Gao et al. 2019). Pure isolates were obtained by transferring the mycelium around the diseased tissues to PDA several times. The colonies were white and cottony after culturing for 7 days. The reverse side of the colony was yellowish white. Basidiospores were hyaline, thin-walled, smooth, 1.7-1.8 x 1.6-1.7 μm (n=30) in size and circular or ellipse in shape, in addition to a dimitic hyphal system (Das et al. 2017). For molecular identification, the genomic DNA of the isolate was extracted using the thermolysis method (Zhang et al. 2010). The ribosomal internal transcribed spacer (ITS) region was amplified using the primer pairs ITS1/ITS4 (White et al. 1990), and the amplified DNA fragments were sequenced. The obtained ITS sequence (GenBank accession No. MW534416) had 99.36% identity with the reference sequence (GenBank accession No. KX013157) of Grammothele lineata Berk. & M.A. Curtis. A phylogenetic tree was constructed with software MEGA7 using the neighbor-joining method, showing that the isolate was grouped in the same clade as G. lineata. To fulfil Koch's postulates, a pathogenicity test was performed using the stems of 6-month-old healthy areca seedlings. Stem surfaces were sterilized with 70% ethanol for 30 s, rinsed three times with sterile water, and gently stabbed with a sterile needle, and then inoculated with a 1-cm-diameter colonized PDA disk from a 7-day culture on wounds, moistened with wet cotton, and wrapped with a fresh plastic wrap. Plants inoculated with sterile PDA medium plugs were used as a control. The inoculation assay was carried out twice, with five plants in both control and treatment in each test. After 20 days, the stems of the plants inoculated with the pathogen exhibited rotten symptoms, and the leaves began to become yellow and shrunken, while the control plants had only the surface of the stems discolored slightly and the inner tissue was undamaged. The fungus was re-isolated from the infected stems. Based on the morphological observations and ITS sequence analysis, the isolate was identified as G. lineata. As far as we know, this is the first report of G. lineata causing the stem rot of areca in China.
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http://dx.doi.org/10.1094/PDIS-01-21-0218-PDNDOI Listing
April 2021

HPV E7 affects the function of cervical cancer cells via the TAL1/lnc‑EBIC/KLHDC7B axis.

Oncol Rep 2021 May 24;45(5). Epub 2021 Mar 24.

Institute of Maternal and Child Health, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430016, P.R. China.

High‑risk human papillomavirus (HPV)16 and 18 are the primary cause of cervical cancer (CC) and long non‑coding RNAs (lncRNAs/lncs) are often abnormally expressed in patients with CC. The authors' previous study indicated that oncogenic enhancer of zeste homolog 2 (EZH2)‑binding lncRNA in cervical cancer (lnc‑EBIC) serves a role in the tumorigenic activity of the HPV E6 protein in patients with CC. However, whether HPV E7 affects the development of CC through lnc‑EBIC, and the potential mechanisms underlying this remains unclear. Therefore, the present study investigated the effects of lnc‑EBIC and HPV E7 in cervical cancer cell lines HeLa, CaSki and C33A . CCK‑8, EdU and DAPI staining assays, flow cytometry, RT‑qPCR, western blotting and Transwell assay were performed on these cell lines. The results revealed that exogenous expression of HPV16/18 E7 significantly promoted lnc‑EBIC expression, and conversely, lnc‑EBIC was downregulated by silencing endogenous HPV16/18 E7 expression in corresponding CaSki and HeLa cells. Overexpression of lnc‑EBIC significantly increased cellular proliferation, migration and invasion, and inhibited apoptosis in HPV‑ C33A cells. The tumorigenic effects of HPV16/18 E7 in corresponding CaSki and HeLa cells were significantly blocked by the silencing of lnc‑EBIC expression. Molecular analysis revealed that HPV16/18 E7 depended on TAL BHLH transcription factor 1, erythroid differentiation factor inhibition to promote lnc‑EBIC expression, which also resulted in the upregulation of oncogenic Kelch domain‑containing 7B (KLHDC7B) in corresponding CaSki and HeLa cells. Additionally, KLHDC7B knockdown blocked the tumor‑promotive effects of lnc‑EBIC in HPV C33A cells. Collectively, the results of the present study indicated that lnc‑EBIC acts as an oncogenic lncRNA by enhancing KLHDC7B expression in HPV and HPV CC cells, and can be exploited by HPV16/18 E7 to accelerate tumorigenic activity in CC. These results further revealed that the lnc‑EBIC/KLHDC7B axis represents a novel molecular mechanism and potential therapeutic target for CC.
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http://dx.doi.org/10.3892/or.2021.8002DOI Listing
May 2021

Butterfly-packaged multi-channel interference widely tunable semiconductor laser with improved performance.

Opt Express 2021 Mar;29(5):6344-6355

A multi-channel interference (MCI) widely tunable semiconductor laser is described in detail with improved performance in this paper. The MCI laser without the common phase section was packaged into a standard 14-pin butterfly package. The device realized a tuning range of more than 40 nm with side mode suppression ratios (SMSRs) higher than 48 dB and about 7 dBm fiber power. By making the gain section and the phase sections to be surface ridge waveguides, threshold currents of the laser have become less than 18 mA across the tuning range. Besides, tuning characteristics of the MCI laser were experimentally studied in detail for the first time. The MCI laser can be treated as a combination of eight Fabry-Pérot (FP) cavity lasers which share the same gain section. It is found that when the eight arm phase sections are completely in phase at the lasing wavelength, the operating currents are at maxima of the output power curves. The relationship between the lasing wavelength and the injection currents of the eight arm phase sections has been introduced and analyzed.
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http://dx.doi.org/10.1364/OE.414376DOI Listing
March 2021

A novel homozygous nonsense mutation in zona pellucida 1 (ZP1) causes human female empty follicle syndrome.

J Assist Reprod Genet 2021 Mar 5. Epub 2021 Mar 5.

State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.

Purpose: To identify a pathogenic gene mutation in a female infertility proband characterized by empty follicle syndrome (EFS) and explore the genetic cause of EFS.

Methods: Whole exome sequencing (WES) was performed to identify the candidate pathogenic mutation. Sanger sequencing was used to validate the mutation in family members. The pathogenicity of the identified variant and its possible effects on the protein were evaluated with in silico tools. Immunofluorescence staining was used to study the possible mechanism of the mutation on affected oocyte.

Results: We identified a family with a novel homozygous nonsense mutation in zona pellucida 1 (ZP1) (c.199G > T [p.Glu67Ter]). Based on bioinformatics analysis, the mutation was predicted to be pathogenic. This variant generates a premature stop codon in exon 2 at the 199th nucleotide, and was inferred to result in a truncated ZP1 protein of 67 amino acids at the ZP-N1 domain. An in vitro study showed that the oocyte of the EFS proband was degenerated and the zona pellucida was absent. Additionally, the mutant ZP1 proteins were localized in the cytoplasm of the degenerated oocyte but not at the surface.

Conclusions: The novel mutation in ZP1 is a genetic cause of female infertility characterized by EFS. Our finding expands the genetic spectrum for EFS and will help justify the EFS diagnosis in patients.
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http://dx.doi.org/10.1007/s10815-021-02136-xDOI Listing
March 2021

Synthesis, structure, electrochemistry and magnetism of cobalt-, nickel- and zinc-containing [M(OH)(HO)(α-SiWO)] (M = Co, Ni, and Zn).

Dalton Trans 2021 Mar 26;50(11):3923-3930. Epub 2021 Feb 26.

Jacobs University, Department of Life Sciences and Chemistry, Campus Ring 1, 28759 Bremen, Germany.

Interaction of the trilacunary 9-tungstosilicate [A-α-SiWO] with cobalt(ii), nickel(ii) and zinc(ii) ions in pH 9 aqueous medium at room temperature led to the formation of the respective M-containing heteropolytungstates [M(OH)(HO)(α-SiWO)] (M = Co (1), Ni (2), and Zn (3)). Polyanions 1-3 were characterized in the solid state by single-crystal XRD, FT-IR spectroscopy, and thermogravimetric and elemental analyses. Electrochemical studies showed that the Co ions in 1 can be oxidized to Co and the CVs of the W centers of the polyanions feature well-defined and chemically reversible reduction waves. Magnetic measurements on 1 and 2 showed paramagnetism with complex ferromagnetic and antiferromagnetic interactions. A model was presented for extracting the exchange constants for the magnetic exchange interaction.
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http://dx.doi.org/10.1039/d0dt03392hDOI Listing
March 2021

Reversible room-temperature phosphorescence in response to light stimulation based on a photochromic copolymer.

Chem Commun (Camb) 2021 Mar 26;57(25):3154-3157. Epub 2021 Feb 26.

Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.

In the field of intelligent luminescence materials, it is a great challenge to regulate organic room-temperature phosphorescence by light irradiation. Herein, benzothiadiazole was modified with viologen derivatives and copolymerized to achieve amorphous RTP emission. The photo-stimulated color change in both emission and absorption has a good reversibility after cycles of light irradiation.
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http://dx.doi.org/10.1039/d1cc00613dDOI Listing
March 2021

The Recurrent Mutation in PATL2 Inhibits Its Degradation Thus Causing Female Infertility Characterized by Oocyte Maturation Defect Through Regulation of the Mos-MAPK Pathway.

Front Cell Dev Biol 2021 4;9:628649. Epub 2021 Feb 4.

State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Suzhou Affiliated Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Nanjing, China.

PAT1 homolog 2 (PATL2), encoding an RNA-binding protein, is a repressor involved in the translational regulation of maternal mRNAs during oocyte maturation. Previous studies have reported mutations in those led to female infertility with oocyte maturation arrest; however, the mechanisms by which mutations affected meiotic maturation remained unclear. Here, we identified several novel and recurrent mutations of in patients with similar phenotype, and chose the missense mutation c.649 T>A p.Tyr217Asn in (PATL2) as a typical to investigate the underlying mechanisms. We confirmed that this mutation disturbed oocyte maturation and observed morphological defects of large polar body, symmetrical division and abnormal spindle after microinjection of corresponding mutated mRNA. We further evaluated the effect of the PATL2 mutation in 293T cells, and found this mutation decreased the ubiquitination level and degradation of PATL2. Then, abnormally increased PATL2 bound mRNAs of Mos, an upstream activator of mitogen activated protein kinase (MAPK), to regulate its translational activity and subsequently impaired MAPK signaling pathway and oocyte meiosis. These results dissented from the previous view that mutations reduced their expression and highlight the role of PATL2 in translational regulation of Mos and its association with MAPK signaling pathway during oocyte meiotic maturation.
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http://dx.doi.org/10.3389/fcell.2021.628649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890943PMC
February 2021

Insight-HXMT observations of jet-like corona in a black hole X-ray binary MAXI J1820+070.

Nat Commun 2021 Feb 15;12(1):1025. Epub 2021 Feb 15.

Department of Physics, Tsinghua University, Beijing, PR China.

A black hole X-ray binary produces hard X-ray radiation from its corona and disk when the accreting matter heats up. During an outburst, the disk and corona co-evolves with each other. However, such an evolution is still unclear in both its geometry and dynamics. Here we report the unusual decrease of the reflection fraction in MAXI J1820+070, which is the ratio of the coronal intensity illuminating the disk to the coronal intensity reaching the observer, as the corona is observed to contrast during the decay phase. We postulate a jet-like corona model, in which the corona can be understood as a standing shock where the material flowing through. In this dynamical scenario, the decrease of the reflection fraction is a signature of the corona's bulk velocity. Our findings suggest that as the corona is observed to get closer to the black hole, the coronal material might be outflowing faster.
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http://dx.doi.org/10.1038/s41467-021-21169-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884741PMC
February 2021

Body mass index and all-cause mortality in patients with cardiogenic shock: A systematic review and meta-analysis.

Am J Emerg Med 2021 May 29;43:97-102. Epub 2021 Jan 29.

Department of Cardiology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China; Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, PR China. Electronic address:

Background: The association between body mass index (BMI) and all-cause mortality of patients with Cardiogenic Shock (CS) is still controversial. The objective of this analysis is to summarize the available evidence of this association and perform meta-analysis using adjusted estimates.

Methods: PubMed, Embase and Cochrane databases were systematically searched for eligible studies up to July 2020. Studies were considered eligible if they described the association between BMI and all-cause mortality of patients with CS, and those reporting adjusted estimates were included in the meta-analysis.

Results: Three studies were identified and included total 345,281 participants. The pooled hazard ratio of all-cause mortality was 0.88(95% confidence interval (CI): 0.71-1.08, P = 0.23) when compared obesity with non-obese. In subgroup analysis, A subgroup analysis based on geographic region showed that obese patients had lower mortality compared with non-obese patients (OR = 0.71,95% CI 0.65-0.77, P < 0.00001) in USA, developed country and the retrospective study. Heterogeneity was not explained in pre-specified subgroups analysis.

Conclusion: Obesity was associated with increased adjusted all-cause mortality of patients with Cardiogenic Shock when compared to non-obese. Unexplained heterogeneity and suboptimal quality of studies limit the strength of the results. This seemingly paradoxical finding needs to be confirmed with further research.
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http://dx.doi.org/10.1016/j.ajem.2021.01.003DOI Listing
May 2021

Genetic etiologic analysis in 74 Chinese Han women with idiopathic premature ovarian insufficiency by combined molecular genetic testing.

J Assist Reprod Genet 2021 Apr 4;38(4):965-978. Epub 2021 Feb 4.

State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.

Purpose: To identify the disease-causing genes of Chinese Han women with idiopathic premature ovarian insufficiency (POI).

Methods: Seventy-four Chinese Han women with idiopathic POI were collected to analyze the genetic etiology. Triplet repeat-primed polymerase chain reaction (TP-PCR) was performed to screen the FMR1 (CGG)n premutation, and then 60 POI-related genes were sequenced by targeted next-generation sequencing (NGS) in POI patients with normal FMR1.

Results: A total of one patient (1/74) with FMR1 premutation was identified. Targeted NGS revealed that 15.07% (11/73) patients had pathogenic or likely pathogenic variants of Mendelian genes (FOXL2, EIF2B2, CYP17A1, CLPP, MCM9, GDF9, MSH5, ERCC6, POLG). Ten novel variants in six Mendelian genes were identified, such as CLPP c.355A>C (p.I119L) and c.688A>C (p.M230L), MCM9 c.1157C>T (p.T386M) and c.1291A>G (p.M431V), GDF9 c. 238C>T (p.Q80X), MSH5 c.604G>C (p.G202R) and c.2063T>C (p.I688T), ERCC6 c.C1769C>T (p.P590L), POLG c.2832G>C (p.E944D), and c.2821A>G (p.I941V).

Conclusion: This study suggested targeted NGS was an efficient etiologic test for idiopathic POI patients without FMR1 premutation and enriched the variant spectrum of POI-related genes.
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http://dx.doi.org/10.1007/s10815-021-02083-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079602PMC
April 2021

Biomedical Photoacoustic Imaging with Unknown Spatially Distributed Ultrasound Sensor Array.

IEEE Trans Biomed Eng 2021 Feb 3;PP. Epub 2021 Feb 3.

Objective: With the growth of interest in different medical study on biological function, non-invasive photoacoustic imaging of biological tissue attracts the interests for researchers. To eliminate the limited angle effect of photoacoustic imaging based on ultrasound linear array, spatially distributed ultrasound sensor array is applied. The accurate sensor array position determines the quality of the imaging results. In this study, we proposed three methods based on photoacoustic and ultrasound signals to enhance the imaging quality using a 256-element full-ring array.

Methods: Groups of photoacoustic and ultrasound signals are used to regress the position of each element sensor.

Result: In phantom study and mouse brain study, photoacoustic imaging results can both yield details clearly with average error rate of less than 1% (50 μm).

Conclusion: The performance of our three methods have proved that they can be potentially applied to other ultrasound-based medical imaging studies with unknown distributed positions of sensor array to enhance the imaging quality.

Significance: The proposed methods can contribute to precise biomedical imaging with unknown distributed positions of sensor array in different application scenarios.
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http://dx.doi.org/10.1109/TBME.2021.3056715DOI Listing
February 2021

Octave-spanning Kerr frequency comb generation with stimulated Raman scattering in an AlN microresonator.

Opt Lett 2021 Feb;46(3):540-543

Octave-spanning optical frequency combs (OFCs) are essential for various applications, such as precision metrology and astrophysical spectrometer calibration. In this Letter, we demonstrate, for the first time to our knowledge, the generation of octave-spanning Kerr frequency combs ranging from 1150 to 2400 nm in aluminum nitride (AlN) microring resonators, by pumping the modes at 250 mW on-chip power. By simply adjusting the pump detuning, we observe the transition and coexistence of Kerr OFC and stimulated Raman scattering. For the mode in the same device, a broadband Raman-assisted frequency comb is demonstrated by adjusting the pump power and tuning. These results indicate a crucial development for the fundamentals of nonlinear dynamics and comb applications in AlN.
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http://dx.doi.org/10.1364/OL.416460DOI Listing
February 2021

Clinical characteristics, rates of blindness, and geographic features of PACD in China.

Can J Ophthalmol 2021 Jan 21. Epub 2021 Jan 21.

The Eye Hospital of Wenzhou Medical University, Wenzhou, China.

Objective: To analyze the rates of blindness with the demographics and clinical characteristics of patients with primary angle-closure disease (PACD) to provide a comprehensive epidemiologic reference in China.

Methods: A retrospective analysis was conducted in the Chinese Glaucoma Study Consortium database, which is a national multicenter glaucoma research alliance of 111 hospitals participating between December 21, 2015 and September 9, 2018. The diagnosis of PACD was made by qualified physicians through examination. Comparison of sex, age, family history, subtypes of PACD, and blindness were analyzed.

Results: A total of 5762 glaucoma patients were included, of which 4588 (79.6%) had PACD. Of PACD patients, 72.1% were female with the sex ratio (F/M) of 2.6, and the average age of patients was 63.8±9.3 years with the majority between 60 and 70 years. Additionally, 30% of these patients had low vision in one eye, 8.8% had low vision in both eyes, 1.7% had blindness in one eye, and 0.3% had blindness in both eyes. There were statistical differences with regards to age between male and female patients with PACD, with male patients being older on average. Primary angle-closure glaucoma was more commonly diagnosed in males (60%) compared to females (35.9%), whereas acute primary angle closure (APAC) was more commonly diagnosed in females (54.3%) compared to males (37.7%). The visual acuity in APAC patients was lower and the rate of low vision and blindness was higher than other subtypes.

Conclusion: PACD was the major type of glaucoma in Chinese hospitals. There were more female patients with PACD, mostly between 60 and 70 years old, with higher rates of APAC in women. APAC resulted in the worst visual outcomes of all PACD subtypes.
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http://dx.doi.org/10.1016/j.jcjo.2020.12.010DOI Listing
January 2021

The complete chloroplast genome of a critically endangered tree species, (Dipterocarpaceae).

Mitochondrial DNA B Resour 2020 Oct 21;5(3):3575-3576. Epub 2020 Oct 21.

College of Ecology and Environment, Center for Terrestrial Biodiversity of the South China Sea, Hainan University, Haikou, China.

Tardieu is a critically endangered tree species distributed in limited regions in Thailand, Vietnam and Hainan Island of China. In this study, we reported the complete chloroplast genome sequence of . assembled from pair-end reads generated by Illumina sequencing. The circular chloroplast genome is 151,407 bp in length and comprises a large single copy region of 84,352 bp, a small single copy region of 19,733 bp, and a pair of inverted repeats of 23,661 bp each. The complete genome encodes 129 functional genes, including 83 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. The overall GC content of the chloroplast genome was 37.4%. Phylogenetic analysis showed that . is sister to . with strong bootstrap support.
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http://dx.doi.org/10.1080/23802359.2020.1829123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782000PMC
October 2020

The complete chloroplast genome sequence of (Clusiaceae).

Mitochondrial DNA B Resour 2020 Aug 25;5(3):3206-3207. Epub 2020 Aug 25.

College of Ecology and Environment, Hainan University, Haikou, China.

Champ. ex Benth is rich in bioactive molecules with immense remedial qualities and has pharmaceutical potentials. In this study, we reported the complete chloroplast genome (cpDNA) of , which is 156,577 bp in length, including a large single copy region (LSC) of 85,393 bp, a small single copy region (SSC) of 17,064 bp, and a pair of inverted repeats (IR) regions of 27,060 bp inserted between LSC and SSC. 129 genes are encoded, including 83 protein-encoding genes, 8 ribosomal RNA genes, and 36 transfer RNA genes. The overall GC content of the chloroplast genome is 36.2%, wherein the corresponding values in the LSC, SSC and IR regions are 33.6%, 30.3%, and 42.2%, respectively. Phylogenetic analysis showed that is sister to with strong bootstrap support.
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http://dx.doi.org/10.1080/23802359.2020.1810162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782034PMC
August 2020

Isolation and characterization of twelve polymorphic microsatellite markers in the endangered (Dipterocarpaceae).

Ecol Evol 2021 Jan 2;11(1):4-10. Epub 2020 Dec 2.

College of Ecology and Environment Hainan University Haikou China.

Microsatellite markers were isolated and characterized for Merrill & Chun, an endangered tree species with scattered distribution in Hainan Island and northern Vietnam. Twenty-six microsatellite markers were developed based on next-generation sequencing data and were genotyped by capillary electrophoresis on an ABI 3730xl DNA Analyzer. Twelve markers were found to be polymorphic in . GENODIVE analyses indicated that the number of alleles ranged from 2 to 6 per locus, and the observed and expected heterozygosity varied from 0 to 0.755 and from 0.259 to 0.779, respectively. Primer transferability was tested with Hand.-Mazz. and Tardieu, in which 3 and 7 microsatellite markers were found to be polymorphic, separately. The results showed that and had similar levels of genetic diversity. A neighbor joining dendrogram clustered all individuals into two major groups, one of which was exclusively constituted by , while the other consisted of two subgroups, corresponding to and , respectively. The 12 polymorphic microsatellite markers could be applied to study genetic diversity, population differentiation, mating system, and fine-scale spatial genetic structures of as well as its close relatives, facilitating the conservation and restoration of these endangered but valuable species.
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http://dx.doi.org/10.1002/ece3.7077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790650PMC
January 2021

Roles of DNA Methylation in Cold Priming in Tartary Buckwheat.

Front Plant Sci 2020 7;11:608540. Epub 2020 Dec 7.

Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.

Plants experience a wide array of environmental stimuli, some of which are frequent occurrences of cold weather, which have priming effects on agricultural production and agronomic traits. DNA methylation may act as an epigenetic regulator for the cold response of Tartary buckwheat (). Combined with long-term field observation and laboratory experiments, comparative phenome, methylome, and transcriptome analyses were performed to investigate the potential epigenetic contributions for the cold priming of Tartary buckwheat variety Dingku1. Tartary buckwheat cv. Dingku1 exhibited low-temperature resistance. Single-base resolution maps of the DNA methylome were generated, and a global loss of DNA methylation was observed during cold responding in Dingku1. These sites with differential methylation levels were predominant in the intergenic regions. Several hundred genes had different DNA methylation patterns and expressions in different cold treatments (cold memory and cold shock), such as , , and . The application of a DNA methylation inhibitor caused a change of the free lysine content, suggesting that DNA methylation can affect metabolite accumulation for Tartary buckwheat cold responses. The results of the present study suggest important roles of DNA methylation in regulating cold response and forming agronomic traits in Tartary buckwheat.
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http://dx.doi.org/10.3389/fpls.2020.608540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750358PMC
December 2020

A solid lipid coated calcium peroxide nanocarrier enables combined cancer chemo/chemodynamic therapy with O/HO self-sufficiency.

Acta Biomater 2021 03 6;122:354-364. Epub 2021 Jan 6.

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

The unfavorable factors in tumor microenvironment such as hypoxia and limited HO levels greatly impede the anticancer efficacy of chemotherapy and chemodynamic therapy (CDT). To address these issues and achieve O/HO-sufficient chemo/chemodynamic combination therapy, we synthesized a solid lipid monostearin coated calcium peroxide (CaO) nanocarrier for the co-delivery of a chemotherapeutic drug doxorubicin (DOX) and a biocompatible Fenton catalyst iron-oleate complex. Specifically, the solid lipid shells of nanoparticles could disintegrate in lipase-overexpressed cancer cells to release iron-oleate and expose CaO cores. Afterwards, the uncovered CaO responded to the acidic aqueous environment within cancer cells, leading to the release of DOX molecules and generation of HO. Based on Fenton reactions, Fe liberated from iron-oleate reacted with HO to produce O for hypoxia-relieved chemotherapy, and Fe for the catalytic generation of hydroxyl radical to initiate CDT. Both treatments synergistically contribute to the enhanced antitumor outcomes.
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http://dx.doi.org/10.1016/j.actbio.2020.12.036DOI Listing
March 2021

Room-Temperature Phosphorescence Enabled through Nacre-Mimetic Nanocomposite Design.

Adv Mater 2021 Feb 21;33(5):e2005973. Epub 2020 Dec 21.

A3BMS Lab-Active, Adaptive and Autonomous Bioinspired Materials, Institute for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Straße 31, Freiburg, 79104, Germany.

A generic, facile, and waterborne strategy is introduced to fabricate flexible, low-cost nanocomposite films with room-temperature phosphorescence (RTP) by incorporating waterborne RTP polymers into self-assembled bioinspired polymer/nanoclay nanocomposites. The excellent oxygen barrier of the lamellar nanoclay structure suppresses the quenching effect from ambient oxygen (k ) and broadens the choice of polymer matrices towards lower glass transition temperature (T ), while providing better mechanical properties and processability. Moreover, the oxygen permeation and diffusion inside the films can be fine-tuned by varying the polymer/nanoclay ratio, enabling programmable retention times of the RTP signals, which is exploited for transient information storage and anti-counterfeiting materials. Additionally, anti-interception materials are showcased by tracing the interception-induced oxygen history that interferes with the preset self-erasing time. Merging bioinspired nanocomposite design with RTP materials contributes to overcoming the inherent limitations of molecular design of organic RTP compounds, and allows programmable temporal features to be added into RTP materials by controlled mesostructures. This will assist in paving the way for practical applications of RTP materials as novel anti-counterfeiting materials.
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http://dx.doi.org/10.1002/adma.202005973DOI Listing
February 2021

Interphotoreceptor Retinol-Binding Protein Ameliorates Diabetes-Induced Retinal Dysfunction and Neurodegeneration Through Rhodopsin.

Diabetes 2021 Mar 17;70(3):788-799. Epub 2020 Dec 17.

Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK

Patients with diabetes often experience visual defects before any retinal pathologies are detected. The molecular mechanism for the visual defects in early diabetes has not been elucidated. Our previous study reported that in early diabetic retinopathy (DR), rhodopsin levels were reduced due to impaired 11--retinal regeneration. Interphotoreceptor retinol-binding protein (IRBP) is a visual cycle protein and important for 11--retinal generation. IRBP levels are decreased in the vitreous and retina of DR patients and animal models. To determine the role of IRBP downregulation in the visual defects in early DR, we induced diabetes in transgenic mice overexpressing IRBP in the retina. IRBP overexpression prevented diabetes-induced decline of retinal function. Furthermore, IRBP overexpression also prevented decreases of rhodopsin levels and 11--retinal generation in diabetic mice. Diabetic IRBP transgenic mice also showed ameliorated retinal oxidative stress, inflammation, apoptosis, and retinal degeneration compared with diabetic wild-type mice. These findings suggest that diabetes-induced IRBP downregulation impairs the regeneration of 11--retinal and rhodopsin, leading to retinal dysfunction in early DR. Furthermore, increased 11--retinal-free opsin constitutively activates the phototransduction pathway, leading to increased oxidative stress and retinal neurodegeneration. Therefore, restored IRBP expression in the diabetic retina may confer a protective effect against retinal degeneration in DR.
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http://dx.doi.org/10.2337/db20-0609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897347PMC
March 2021

MicroRNA-22 Inhibits the Apoptosis of Vascular Smooth Muscle Cell by Targeting p38MAPKα in Vascular Remodeling of Aortic Dissection.

Mol Ther Nucleic Acids 2020 Dec 25;22:1051-1062. Epub 2020 Aug 25.

Department of Vascular Surgery, Changhai Hospital, Navy Medical University, Shanghai 200433, China.

MicroRNA 22 (miR-22) was found in diverse cardiovascular diseases to have a role in regulating multiple cellular processes. However, the regulatory role of miR-22 in aortic dissection (AD) was still unclear. The miR-22 expression in human aorta was explored. A series of mimic, inhibitor, or small interfering RNA (siRNA) plasmids were delivered into vascular smooth muscle cells (VSMCs) to explore the effects of miR-22 and p38 mitogen-activated protein kinase α (p38MAPKα) in controlling VSMC apoptosis . In addition, a mouse AD model was established, and histopathologic analyses were performed to evaluate the regulatory effects of miR-22. Reduced miR-22 and increased apoptosis of VSMCs was seen in human AD aorta. Downregulation of miR-22 increased the apoptosis of VSMCs . Bioinformatics analyses revealed that p38MAPKα was a target of miR-22. Inhibiting p38MAPKα expression could reverse the apoptosis of VSMCs induced by miR-22 downregulation. Knockdown of miR-22 in the AD mouse model significantly promoted the development of AD. Our data underscore the importance of vascular remodeling and VSMC function in AD. miR-22 may represent a new therapeutic approach for AD by regulating the apoptosis of VSMCs through the MAPK signaling pathway.
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http://dx.doi.org/10.1016/j.omtn.2020.08.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691156PMC
December 2020

TRPS1 and YAP1 Regulate Cell Proliferation and Drug Resistance of Osteosarcoma via Competitively Binding to the Target of circTADA2A - miR-129-5p.

Onco Targets Ther 2020 1;13:12397-12407. Epub 2020 Dec 1.

Department of Orthopaedics, CR & WISCO General Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei 430000, People's Republic of China.

Introduction: The yes-associated protein (YAP) and trichorhinophalangeal syndrome 1 (TRPS1) have been reported to account for the pathogenesis of cancers and may play an important role in osteosarcoma (OS). This study intended to investigate the modulatory effect and relationship of TRPS1 and YAP1 in OS cells.

Methods: The expression difference of YAP1 and TRPS1 in OS cells was measured. Then, the effect of circTADA2A silence on YAP1 and TRPS1 expression as well as OS proliferation and drug resistance was estimated.

Results: TRPS1 and YAP1 were upregulated in OS cell lines, and TRPS1 and YAP1 were highly expressed in MG63 and U2OS cells, respectively. The cell proliferation of MG63 was lower than that of U2OS, but the opposite result was observed in the presence of cisplatin (DDP). CircTADA2A was upregulated while miR-129-5p was downregulated in MG63 and U2OS cells compared. Besides, circTADA2A knockdown inhibited cell proliferation and reduced DDP resistance in both MG63 and U2OS. MiR-129-5p was increased but TRPS1 and YAP1 were decreased by circTADA2A knockdown. Meanwhile, circTADA2A knockdown reduced TRPS1 protein expression but enhanced phosphorylated (p)-YAP1. In xenograft OS tumor mice, circTADA2A knockdown inhibited tumor growth in the absence or presence of DDP. Finally, miR-129-5p could bind to circTADA2A, TRPS1 and YAPS.

Discussion: CircRNA TADA2A could target miR-129-5p, which was competitively bound by TRPS1 and YAP1, thereby regulating OS cell proliferation and drug resistance.
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http://dx.doi.org/10.2147/OTT.S276953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719346PMC
December 2020