Publications by authors named "Xiang Liu"

1,340 Publications

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[Progress in enhancing electron transfer rate between exoelectrogenic microorganisms and electrode interface].

Sheng Wu Gong Cheng Xue Bao 2021 Feb;37(2):361-377

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Exoelectrogenic microorganisms are the research basis of microbial electrochemical technologies such as microbial fuel cells, electrolytic cells and electrosynthesis. However, their applications are restricted in organic degradation, power generation, seawater desalination, bioremediation, and biosensors due to the weak ability of biofilm formation and the low extracellular electron transfer (EET) efficiency between exoelectrogenic microorganisms and electrode. Therefore, engineering optimization of interaction between exoelectrogenic microorganisms and electrode interface recently has been the research focus. In this article, we review the updated progress in strategies for enhancing microbe-electrode interactions based on microbial engineering modifications, with a focus on the applicability and limitations of these strategies. In addition, we also address research prospects of enhancing the interaction between electroactive cells and electrodes.
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http://dx.doi.org/10.13345/j.cjb.200281DOI Listing
February 2021

Alkyl Titanate-Modified Graphene Oxide as Friction and Wear Reduction Additives in PAO Oil.

ACS Omega 2021 Feb 28;6(5):3840-3846. Epub 2021 Jan 28.

PetroChina Lubricant Company, Chaoyang District, Beijing 100020, P R of China.

Diacetoxy-stearoyloxy-titanium-modified graphene oxide (Titanate-GO) was successfully prepared using titanium tetraisopropanolate, stearic acid, acetic acid, and graphene oxide (GO). The morphology and structure of the as-prepared materials were tested by FT-IR, Raman, TG/DSC, SEM, and TEM instruments. The results indicate that long alkyl titanium chains have been grafted on the surface of a GO sheet, which guaranteed the dispersibility of Titanate-GO in PAO10 base oil. Then, the lubrication properties of Titanate-GO as a lubricating additive in PAO10 base oil were evaluated on a four-ball machine. The results show that the average coefficient of friction and wear scar diameter were reduced by 49.5 and 28.2%, respectively, compared with bare PAO10 base oil. Finally, the lubrication mechanism was discussed based on the Raman analysis, which was carried out on the worn surface of the steel ball.
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http://dx.doi.org/10.1021/acsomega.0c05604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906493PMC
February 2021

A plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID-19 research.

PLoS Biol 2021 02 25;19(2):e3001091. Epub 2021 Feb 25.

Emerging Viruses, Inflammation and Therapeutics Group, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia.

The recent emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the underlying cause of Coronavirus Disease 2019 (COVID-19), has led to a worldwide pandemic causing substantial morbidity, mortality, and economic devastation. In response, many laboratories have redirected attention to SARS-CoV-2, meaning there is an urgent need for tools that can be used in laboratories unaccustomed to working with coronaviruses. Here we report a range of tools for SARS-CoV-2 research. First, we describe a facile single plasmid SARS-CoV-2 reverse genetics system that is simple to genetically manipulate and can be used to rescue infectious virus through transient transfection (without in vitro transcription or additional expression plasmids). The rescue system is accompanied by our panel of SARS-CoV-2 antibodies (against nearly every viral protein), SARS-CoV-2 clinical isolates, and SARS-CoV-2 permissive cell lines, which are all openly available to the scientific community. Using these tools, we demonstrate here that the controversial ORF10 protein is expressed in infected cells. Furthermore, we show that the promising repurposed antiviral activity of apilimod is dependent on TMPRSS2 expression. Altogether, our SARS-CoV-2 toolkit, which can be directly accessed via our website at https://mrcppu-covid.bio/, constitutes a resource with considerable potential to advance COVID-19 vaccine design, drug testing, and discovery science.
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http://dx.doi.org/10.1371/journal.pbio.3001091DOI Listing
February 2021

Quantitative Trait Locus Mapping and Identification of Candidate Genes Controlling Flowering Time in L.

Front Plant Sci 2020 3;11:626205. Epub 2021 Feb 3.

State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling, China.

Flowering time plays a vital role in determining the life-cycle period, yield, and seed quality of rapeseed ( L.) in certain environments. Quantitative trait locus (QTL) mapping to identify the genetic architecture of genes controlling flowering time helps accelerate the early maturity breeding process. In this study, simple sequence repeats (SSR) and specific-locus amplified fragment sequencing (SLAF-seq) technologies were adopted to map the QTLs for flowering time in four environments. As a result, three target intervals, , , and were identified. Among this, was considered as a novel interval, and as stable regions. Based on the parental re-sequencing data, 7,022 single nucleotide polymorphisms (SNPs) and 2,195 insertion-deletions (InDels) between the two parents were identified in these three target regions. A total of 186 genes possessed genetic variations in these intervals, 14 of which were related to flowering time involved in photoperiod, circadian clock, vernalization, and gibberellin pathways. Six InDel markers linked to flowering time were developed in the three target intervals, indicating that the results were credible in this study. These results laid a good foundation for further genetic studies on flowering-time regulation in L.
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http://dx.doi.org/10.3389/fpls.2020.626205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886670PMC
February 2021

Anesthetic effect of ultrasound-guided multiple-nerve blockade in modified radical mastectomy in patients with breast cancer.

Medicine (Baltimore) 2021 Feb;100(7):e24786

Department of Anesthesiology, The Yancheng Clinical College of Xuzhou Medical University, The First People's Hospital of Yancheng, No. 166 West Yulong Road, Yancheng, P. R. China.

Introduction: Routine anesthesia modality for modified radical mastectomy (MRM) includes general anesthesia (GA), epidural blockade-combined GA and nerve blockade-combined GA. However, GA has been associated with postoperative adverse effects such as vertigo, postoperative nausea and vomiting and requirement for postoperative analgesia, which hinders recovery and prognosis. Moreover, combined blockade of thoracic paravertebral nerves or intercostal nerves and adjuvant basic sedation for massive lumpectomy provided perfect anesthesia and reduced opioid consumption, whereas the excision coverage did not attain the target of MRM. Regional anesthesia strategies involving supplementation of analgesics in ultrasound-guided multiple nerve blocks have garnered interests of clinicians. Nevertheless, the precise effects of intercostal nerves, brachial plexus and supraclavicular nerves in MRM in patients with breast cancer remain obscure.

Methods: Eighty female patients with breast cancer scheduled for MRM were recruited in the present trial between May, 2019 and Dec., 2019 in our hospital. The patients ranged from 30 to 65 years of age and 18∼30 kg/m2 in body-mass index, with the American Society of Anesthesiologists I or II. The patients were randomized to ultrasound-guided multiple nerve blocks group and GA group. The patients in multiple nerve blocks group underwent ultrasound guided multiple intercostal nerve blocks, interscalene brachial plexus and supraclavicular nerve blocks, (local anesthesia with 0.3% ropivacaine: 5 ml for each intercostal nerve block, 8 ml for brachial plexus block, 7 mL for supraclavicular nerve block) and basic sedation and intraoperative mask oxygen inhalation. The variations of hemodynamic parameters such as mean arterial pressure, heart rate (HR) and pulse oxygen saturation were monitored. The visual analog scale scores were recorded at postoperative 0 hour, 3 hour, 6 hour, 12 hour and 24 hour in resting state. The postoperative adverse effects, including vertigo, postoperative nausea, and vomiting, pruritus, and urinary retention and so on, as well as the analgesic consumption were recorded.

Conclusions: The ultrasound guided multiple intercostal nerve blocks, brachial plexus and supraclavicular nerve blocks could provide favorable anesthesia and analgesia, with noninferiority to GA and the reduced incidence of adverse effects and consumption of postoperative analgesics.
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http://dx.doi.org/10.1097/MD.0000000000024786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7899908PMC
February 2021

Effectively removing tetracycline from water by nanoarchitectured carbons derived from CO: Structure and surface chemistry influence.

Environ Res 2021 Feb 16:110883. Epub 2021 Feb 16.

School of Resource and Environmental Science, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Wuhan University, Wuhan 430072, China; State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan 430072, PR China. Electronic address:

Understanding of the correlation between physico-chemical property of adsorbent and the adsorption performance of contaminant is very significant for developing high-efficient materials to remove antibiotic contamination from water. In this work, a novel kind of carbon adsorbent (EC) derived from CO and activated ECs with modified structure via a facile chemical method using H and KOH were prepared. The synthetic carbon materials (EC, EC-H, and EC-KOH) were then applied to remove tetracycline (TC). The kinetics of adsorption for these three carbon materials all well fitted the pseudo-second-order kinetic model. The experimental data of adsorption isotherm had good compatibility with Langmuir and Freundlich models (R > 0.90), but the Temkin model was the most applicable for all adsorbents (R > 0.98). A super-high adsorption capacity of EC-KOH obtained from Langmuir fitting was 933.56 mg g, which was much higher than that of EC-H (538.91 mg g) and EC (423.30 mg g), possibly due to a larger specific surface area (S), pore volume, and specific surface chemical structure. Moreover, it was found that surface functional groups and large aperture of adsorbents had a positive effect on adsorption rate. More adsorption sites and surface functional groups of adsorbents were beneficial to enhance the adsorption affinity. These results are of great benefit to the directional control of carbon structure to increase the adsorption performance in rate, capacity, and affinity of antibiotics.
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http://dx.doi.org/10.1016/j.envres.2021.110883DOI Listing
February 2021

Rhynchophylline improves trophocyte mobility potential by upregulating ZEB1 level via the inhibition of miR-141-3p level.

Biosci Biotechnol Biochem 2021 Feb;85(2):280-286

Department of Gynecology, The First People's Hospital of Wenling, Wenling, Zhejiang, China.

Preeclampsia (PE) is characterized by the impaired invasive ability of trophocytes, which can be modulated by microRNAs (miRs). In the current study, the effects of rhynchophylline (Rhy) on the viability and invasive ability of trophocytes were explored by focusing on miR-141-3p/ZEB1 axis. The level of miR-141-3p was modulated in human trophocytes and the changes in cell viability, apoptosis, invasive ability, and ZEB1 level were detected. Then the trophocytes with miR-141-3p overexpression were treated with Rhy and the effects on trophocyte phenotypes were assessed. The induced miR-141-3p level suppressed cell viability, induced apoptosis, and inhibited invasion and ZEB1 level in trophocytes. The treatment of Rhy restored the viability and invasive ability of trophocytes under the overexpression of miR-141-3p, indicating the protective effects of Rhy on trophocytes. The findings in the current study highlighted the protective effects of Rhy on trophocytes during PE progression, which was associated with the inhibition of miR-141-3p.
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http://dx.doi.org/10.1093/bbb/zbaa016DOI Listing
February 2021

WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps.

Front Cell Dev Biol 2020 1;8:630340. Epub 2021 Feb 1.

Department of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Cilia loss and dysfunction is one of the typical pathological features of chronic rhinosinusitis with nasal polyps (CRSwNP). Tryptophan-aspartic acid (W-D) repeat containing planar cell polarity effector (WDPCP) has been proven to be an essential element for ciliogenesis in human nasal epithelium, but its role in the beating of cilia remains unclear. In this study, we sought to investigate the role of WDPCP and its underlying mechanism behind the dysfunction in the beating of cilia in nasal polyp tissue. We demonstrated WDPCP expression in the epithelium of nasal polyps. We also investigated the MAPK/ERK pathway in primary human sinonasal epithelial cells to explore the function of WDPCP. The air-liquid interface culture system was used as a model to verify the role of WDPCP and the MAPK/ERK pathway in the beating of cilia. With the dysfunction of cilia beating, we observed a low expression of WDPCP in the epithelium of nasal polyp tissues. Within the study, we found that WDPCP was critical for mitochondrial biogenesis and mitochondrial function in human sinonasal epithelial cells, possibly due to the activation of the MAPK/ERK pathway. The mitochondrial dysfunction caused by U0126 or lacking WDPCP could be partially recovered by dexamethasone. The low expression of WDPCP in nasal epithelium could affect mitochondria via the MAPK/ERK pathway, which may contribute to the dysfunction in the beating of cilia in CRSwNP.
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http://dx.doi.org/10.3389/fcell.2020.630340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882705PMC
February 2021

Association of UBASH3A polymorphism with chronic spontaneous urticaria in a Chinese Han population.

J Cosmet Dermatol 2021 Feb 14. Epub 2021 Feb 14.

Department of Dermatology, Affiliated Hospital of Jining Medical University, Jining, China.

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http://dx.doi.org/10.1111/jocd.14003DOI Listing
February 2021

Host plant environmental filtering drives foliar fungal community assembly in symptomatic leaves.

Oecologia 2021 Feb 13. Epub 2021 Feb 13.

State Key Laboratory of Grassland Agro-Ecosystem, Institute of Innovation Ecology, Lanzhou University, 222 Tianshui South Road, Lanzhou, 730000, People's Republic of China.

Foliar fungi (defined as all fungal species in leaves after surface sterilization; hereafter, 'FF') are of great importance to host plant growth and health, and can also affect ecosystem functioning. Despite this importance, few studies have explicitly examined the role of host filtering in shaping local FF communities, and we know little about the differences of FF community assembly between symptomatic (caused by fungal pathogens) and asymptomatic leaves, and whether there is phylogenetic congruence between host plants and FF. We examined FF communities from 25 host plant species (for each species, symptomatic and asymptomatic leaves, respectively) in an alpine meadow of the Tibetan Plateau using MiSeq sequencing of ITS1 gene biomarkers. We evaluated the phylogenetic congruence of FF-plant interactions based on cophylogenetic analysis, and examined α- and β-phylogenetic diversity indices of the FF communities. We found strong support for phylogenetic congruence between host plants and FF for both asymptomatic and symptomatic leaves, and a host-caused filter appears to play a major role in shaping FF communities. Most importantly, we provided independent lines of evidence that host environmental filtering (caused by fungal infections) outweighs competitive exclusion in driving FF community assembly in symptomatic leaves. Our results help strengthen the foundation of FF community assembly by demonstrating the importance of host environmental filtering in driving FF community assembly.
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http://dx.doi.org/10.1007/s00442-021-04849-3DOI Listing
February 2021

Children's erythrocyte fatty acids are associated with the risk of islet autoimmunity.

Sci Rep 2021 Feb 11;11(1):3627. Epub 2021 Feb 11.

Health and Well-Being Promotion Unit, Public Health and Welfare Department, Finnish Institute for Health and Welfare, P.O. Box 30, 00271, Helsinki, Finland.

Our aim was to investigate the associations between erythrocyte fatty acids and the risk of islet autoimmunity in children. The Environmental Determinants of Diabetes in the Young Study (TEDDY) is a longitudinal cohort study of children at high genetic risk for type 1 diabetes (n = 8676) born between 2004 and 2010 in the U.S., Finland, Sweden, and Germany. A nested case-control design comprised 398 cases with islet autoimmunity and 1178 sero-negative controls matched for clinical site, family history, and gender. Fatty acids composition was measured in erythrocytes collected at the age of 3, 6, and 12 months and then annually up to 6 years of age. Conditional logistic regression models were adjusted for HLA risk genotype, ancestry, and weight z-score. Higher eicosapentaenoic and docosapentaenoic acid (n - 3 polyunsaturated fatty acids) levels during infancy and conjugated linoleic acid after infancy were associated with a lower risk of islet autoimmunity. Furthermore, higher levels of some even-chain saturated (SFA) and monounsaturated fatty acids (MUFA) were associated with increased risk. Fatty acid status in early life may signal the risk for islet autoimmunity, especially n - 3 fatty acids may be protective, while increased levels of some SFAs and MUFAs may precede islet autoimmunity.
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http://dx.doi.org/10.1038/s41598-021-82200-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878879PMC
February 2021

Metal-modified sludge-based biochar enhance catalytic capacity: Characteristics and mechanism.

J Environ Manage 2021 Apr 9;284:112113. Epub 2021 Feb 9.

Department of Environmental Science and Engineering, Fudan University, Shanghai, 200438, China. Electronic address:

The improvement of the catalytic performance of sludge-based biochar plays an important role in the catalytic application of biochar. This work aimed to use transition metals and rare earth elements (Fe, Ce, La, Al, Ti) to modify sludge and prepare modified biochar with better catalytic performance through pyrolysis. Through the Fourier transform infrared spectrometer, Raman spectrometer, and X-ray photoelectron spectroscopy, the effects of different metal modifications on the surface morphology, molecular structure, element compositions, and valence of elements of biochar were comprehensively investigated. The results showed that metal elements were successfully modified onto the surface of biochar as metal oxides. Although the highest intensity of persistent free radicals was detected in blank-biochar by electron spin resonance, the intensities of hydroxyl radicals catalyzed by modified biochars in HO system were higher than that catalyzed by blank-biochar, indicating that the catalytic performance of modified biochar was mainly related to the metal oxide loaded and the defect structure on the surface of metal-modified biochar. Furthermore, in the HO system, the degradation efficiencies of tetracycline catalyzed by the biochars within 4 h were 51.7% (blank-biochar), 90.7% (Fe-biochar), 69.0% (Ce-biochar), 59.9% (La-biochar), 58.0% (Al-biochar), 58.0% (Ti-biochar), respectively, suggesting that Fe-biochar not only possessed the best catalytic performance but also shortened the reaction time. This research not only provided the possibility for recycling the waste activated sludge, but also proposed a modification method to improve the catalytic performance of biochar.
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http://dx.doi.org/10.1016/j.jenvman.2021.112113DOI Listing
April 2021

Effects of a Caregiver Training Program on Oral Hygiene of Alzheimer's Patients in Institutional Care.

J Am Med Dir Assoc 2021 Feb 9. Epub 2021 Feb 9.

Department of Geriatric Medicine, Henan Provincial People's Hospital, Henan University, Zhengzhou, China. Electronic address:

Objectives: To investigate the effects of a caregiver training program on the oral hygiene of caregivers and patients with Alzheimer's disease (AD) and to identify program components and parameters for accurate assessment of outcomes.

Design: Single-blinded prospective cohort study.

Setting And Participants: Patients with AD and caregivers in nursing homes in the Greater Zhengzhou Area, China.

Methods: Initially 168 AD patient/caregiver pairs were recruited and randomly assigned to control, limited training, and comprehensive training groups. The mini-mental state examination, global deterioration scale, and Katz activities of daily living scale were conducted for patients with AD. Information on participants' oral hygiene habits and general oral health was collected. The modified Quigley-Hein Plaque Index (PI) and Gingival Index (GI) were used to assess oral hygiene and gingival health. Intervention included (1) an educational video showing the role of dental plaque and the modified Bass technique; and (2) caregivers practicing toothbrushing on themselves and patients with AD under professional guidance. Changes in oral hygiene and correlations between patient PI/GI and caregiver PI/GI were analyzed.

Results: After 6 weeks, complete data for 146 AD patient/caregiver pairs were collected. Before enrollment, most patients with AD had very poor oral hygiene. Compared with controls and limited training, only comprehensive training was able to achieve steady reduction in PI and GI scores in patients with AD, which still fell short of desirable levels (PI: 2.46 ± 0.52, GI: 1.24 ± 0.24, week 6). PI and GI scores in caregivers saw steady improvement only through comprehensive training (PI: 1.41 ± 0.38, GI: 0.88 ± 0.19, week 6). Number of training sessions had the greatest influence on both patient PI and GI scores.

Conclusions And Implications: Comprehensive caregiver training on toothbrushing skills is effective in improving the oral hygiene of caregivers and patients with AD in nursing homes. Additional evidence is needed to establish the optimal program structure.
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http://dx.doi.org/10.1016/j.jamda.2020.12.042DOI Listing
February 2021

Obesity paradox in pulmonary hypertension due to left ventricular systolic dysfunction.

Herz 2021 Feb 5. Epub 2021 Feb 5.

Department of Cardiology, The Second Affiliated Hospital of Soochow University, No 1055, Sanxiang Road, 215004, Suzhou City, China.

Objective: Pulmonary hypertension (PH) due to left ventricular systolic dysfunction (PH-HFrEF) is a common heart disease with poor prognosis. In this study, we explored the risk factors for PH-HFrEF and investigated the related factors affecting the prognosis of PH-HFrEF patients.

Methods: The study recruited consecutive patients with PH-HFrEF and systolic pulmonary artery pressure (sPAP) of more than 40 mm Hg with left ventricular ejection fraction (LVEF) of less than 45% on echocardiography. Patients with left ventricular systolic dysfunction (HFrEF) but without PH (sPAP < 30 mmHg and LVEF < 45%) were chosen as the control group. Patients were followed up for 18 months, and major adverse cardiac events (MACE) were recorded.

Results: In total, 93 patients with PH-HFrEF formed the study group and 93 LVEF-matched patients with HFrEF were enrolled as controls. Body mass index (BMI) in PH-HFrEF patients was significantly lower compared with the control group (p < 0.05). Multivariate logistic regression analysis revealed that low BMI was an independent predictor of the presence of PH in patients with HFrEF (p < 0.05). There were 23 (24.7%) MACE in the PH-HFrEF group and 18 (19.4%) MACE in the control group. Cox regression analysis showed that low BMI was an independent predictor of MACE occurrence in the PH-HFrEF group (p < 0.05).

Conclusion: Low BMI appear to be significantly associated with PH occurrence in patients with HFrEF, and is an independent predictor of MACE in patients with PH-HFrEF.
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http://dx.doi.org/10.1007/s00059-021-05023-4DOI Listing
February 2021

Data-driven Boolean Network Inference Using a Genetic Algorithm with Marker-based Encoding.

IEEE/ACM Trans Comput Biol Bioinform 2021 Jan 29;PP. Epub 2021 Jan 29.

The inference of Boolean networks is crucial for analyzing the topology and dynamics of gene regulatory networks. Many data-driven approaches using evolutionary algorithms have been proposed based on time-series data. However, the ability to infer both network topology and dynamics is restricted by their inflexible encoding schemes. To address this problem, we propose a novel Boolean network inference algorithm for inferring both network topology and dynamics simultaneously. The main idea is that, we use a marker-based genetic algorithm to encode both regulatory nodes and logical operators in a chromosome. By using the markers and introducing more logical operators, the proposed algorithm can infer more diverse candidate Boolean functions. The proposed algorithm is applied to five networks, including two artificial Boolean networks and three real-world gene regulatory networks. Compared with other algorithms, the experimental results demonstrate that our proposed algorithm infers more accurate topology and dynamics.
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http://dx.doi.org/10.1109/TCBB.2021.3055646DOI Listing
January 2021

Comparison of Telemedicine Versus In-Person Visits on Impact of Downstream Utilization of Care.

Telemed J E Health 2021 Jan 29. Epub 2021 Jan 29.

Institute for Healthcare Policy and Innovation, University of Michigan, Ann Arbor, Michigan, USA.

Telemedicine use has expanded substantially in recent years. Studies evaluating the impact of telemedicine modalities on downstream office visits have demonstrated mixed results. We evaluated insurance claims of a large commercial payer, Blue Cross Blue Shield of Michigan (BCBSM), to assess the frequency of follow-up visits following encounters initiated via telemedicine versus in-person. We used the BCBSM claim-level data set (2011-2017) to assess encounters in the following places of service: hospital outpatient, doctor's office, patient's home, or psychiatric daycare facility. We identified the primary diagnostic category for 30-day episodes of care using clinical classifications software (CCS) and multilevel clinical classifications software (ML-CCS). Our intervention group consisted of episodes initiated via telemedicine; our control group consisted of episodes initiated in-person. Our primary outcome was the percentage of 30-day episodes with a related visit (encounters occurring within the same period and CCS categories) across CCS categories. Our secondary outcome was the mean related visit rate. The final data set included 4,982,456 patients and 68,148,070 claims, of which 53,853 were telemedicine related. Many episodes did not have related visits (the mean related visit rate was 16%). Telemedicine visits had a higher frequency of related visits across all CCS categories. Episodes of care initiated via telemedicine more frequently generate related visits within a 30-day period. This increased health care utilization could represent excessive care or could reflect expanded access to care. Further research should explore the cause of this increased utilization and potential unintended consequences.
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http://dx.doi.org/10.1089/tmj.2020.0286DOI Listing
January 2021

Polygoni Multiflori Radix Preparat Delays Skin Aging by Inducing Mitophagy.

Biomed Res Int 2021 13;2021:5847153. Epub 2021 Jan 13.

Medical Cosmetology Center, Affiliated Hospital of Traditional Chinese Medicine, Southwest Medical University, Luzhou, Sichuan 64600, China.

Background: As the skin is the largest organ of the human body, it is aging inevitably and produces cosmetic and psychological problems, and even disease. Therefore, the molecular mechanisms related to the prevention of skin aging need to be further explored.

Methods: Aging models were constructed by D-galactose. Mice were administrated with polygoni multiflori radix preparat (PMRP), PMRP and 3-methyladenine, or PMRP and rapamycin intragastrically. The apparent and viscera index of aged rats was measured. Then, the physicochemical property, antioxidant ability, histological structure, mitochondrial membrane potential, ATP and ROS levels, and mitophagy of aged skins were determined. Finally, the expression of PINK1, Parkin, P62, and LC3II/I; apoptosis-related proteins; and the percentage of apoptotic cells were measured.

Results: PMRP relieved skin aging with reducing of thymus index, improvement of pathological damage and histological structure, increase of the expression area of fibrous tissue, the ratio of type I to type III collagen, and antioxidant ability of aged skins. Importantly, PMRP also improved mitochondrial dysfunction with an increase in the content of mitochondrial membrane potential and ATP and a decrease of ROS levels. Moreover, mitophagy was enhanced with the treatment of PMRP when observed using electron microscopy, and the expression of PINK1, Parkin, and LC3I/II was increased with PMRP treatment but P62 expression was decreased. Meanwhile, PMRP alleviated apoptosis with a decrease of apoptotic cell and the expression of Cleaved-cas3, Bax, Cyt-c, AIF, and Smac as well as an increase of Bcl-2 expression.

Conclusion: The results demonstrated that the polygoni multiflori radix preparata may delay skin aging by inducing mitophagy.
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http://dx.doi.org/10.1155/2021/5847153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822667PMC
January 2021

Normalized Protein Catabolic Rate Is a Superior Nutritional Marker Associated With Dialysis Adequacy in Continuous Ambulatory Peritoneal Dialysis Patients.

Front Med (Lausanne) 2020 12;7:603725. Epub 2021 Jan 12.

Division of Nephrology, Department of Medicine, West China Hospital, Sichuan University, Chengdu, China.

Current knowledge of the relationship between normalized protein catabolic rate (nPCR) and dialysis adequacy is limited. Our study aimed to explore the potential relationship between nPCR and dialysis adequacy. In this cross-sectional study, we analyzed the association of nPCR with peritoneal dialysis adequacy in 266 continuous ambulatory peritoneal dialysis (CAPD) patients (mean age 48.6 ± 13.1 years; 50.8% male). The patients were divided into two groups: a dialysis inadequacy group (total weekly Kt/V urea < 1.70) and a dialysis adequacy group (total weekly Kt/V urea≥1.70). We then analyzed the correlation between dialysis adequacy and the patients' primary cause of end-stage renal disease, nutritional and inflammatory markers, and biochemical parameters. Multivariable logistic regression analysis was also used to identify risk factors for inadequate dialysis. We observed a significantly higher level of nPCR (0.98 ± 0.22 vs. 0.79 ± 0.18 g/kg/day, < 0.001) in the dialysis adequacy group, whereas we observed no significant differences among other nutritional markers such as albumin, prealbumin, and transferrin. Correlation analyses revealed that dialysis adequacy was positively associated with residual glomerular filtration rate (rGFR), hemoglobin, serum calcium, and body mass index (BMI), while dialysis adequacy was negatively associated with leak-protein, uric acid, high-sensitivity C-reactive protein, interleukin-6, and serum phosphorus. Furthermore, a logistic regression analysis revealed that gender (male), nPCR <0.815 g/kg/day, higher weight, and rGFR <2.43 mL/min/1.73 m were independent risk factors for inadequate dialysis. Nutritional status is closely associated with dialysis adequacy. Among common nutritional markers, nPCR may be superior for predicting CAPD dialysis adequacy. Gender (male), nPCR <0.815 g/kg/day, higher weight, and rGFR <2.43 mL/min/1.73 m are independent risk factors for dialysis inadequacy in CAPD patients.
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http://dx.doi.org/10.3389/fmed.2020.603725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835658PMC
January 2021

Atomic-Scale Mechanism of Grain Boundary Effects on the Magnetic and Transport Properties of FeO Bicrystal Films.

ACS Appl Mater Interfaces 2021 Feb 26;13(5):6889-6896. Epub 2021 Jan 26.

Tianjin Key Laboratory of Low Dimensional Materials Physics and Processing Technology, School of Science, Tianjin University, Tianjin 300354, China.

In strongly correlated materials, change of the local lattice configuration is expected to tune or even generate new properties otherwise in the ideal bulk materials. For highly spin-polarized materials, the spin-dependent transport is sensitive to the local magnetic structure. Here, the artificial grain boundaries (GBs) with different tilt angles are produced in FeO films using SrTiO bicrystal substrates. The saturation magnetization of FeO bicrystal films is enhanced. The detailed atomic structural results combining with density functional theory calculations reveal that the elongated Fe-O bond length at GBs resulting in the reduction of charge transfer reduces the Fe magnetic moments, which enhances the total magnetic moments of FeO. The in-plane rotation of the FeO lattice on bicrystal substrates alters the magnetization processes. Especially, the FeO bicrystal film with a tilt angle of 22.6° shows strong in-plane magnetic anisotropy due to the zigzag GBs. The altered magnetic anisotropy of FeO bicrystal films enhances the anisotropic magnetoresistance. The findings reveal the mechanism of GBs on the magnetic and transport properties and manifest that the strategy of utilizing GBs can tune the physical properties in highly spin-polarized materials.
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http://dx.doi.org/10.1021/acsami.0c21705DOI Listing
February 2021

Acid treatment enhances phosphorus release and recovery from waste activated sludge: Performances and related mechanisms.

Sci Total Environ 2021 Apr 14;763:142947. Epub 2020 Oct 14.

Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China. Electronic address:

Waste activated sludge (WAS) has attracted considerable attention as an excellent material for P recovery from sewage. This study took concentrated phosphorus removal sludge as objective, and aimed at providing an effective route to promote the transformation of polyphosphate in sludge pellets to dissolved phosphate. After acid pH adjustment, total dissolved phosphate at pH 3.0 was 37.3-fold higher than that at natural pH. The P distribution results illustrated that acid pH accelerated the degradation of short chain polyphosphate into orthophosphate. Furthermore, cell staining results confirmed that the polyphosphate was sourced from the leakage of intracellular matters. Low field NMR and rheology properties analysis were adopted to illustrate that acid pH treatment further improved WAS dewatering performances. Accompanied with the reduction of heavy metals in WAS pellets, the acid pH treatment was also beneficial for WAS subsequent treatment or final disposal. Lastly, Fe addition was proposed as favorable P recovery method, and spherical nanometric materials of FePO crystal was obtained accordingly.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142947DOI Listing
April 2021

Electric field induced reversal of spin polarization, magnetic anisotropy and tailored Dzyaloshinskii-Moriya interaction in underoxidized SrRuO/SrTiO heterostructures.

Phys Chem Chem Phys 2021 Feb;23(4):3008-3018

Tianjin Key Laboratory of Low Dimensional Materials Physics and Preparation Technology, School of Science, Tianjin University, Tianjin 300354, China.

Electric field tailored magnetic properties of the perovskite-type oxide heterostructures are important in spintronic devices with low energy consumption and small size. Here, the electric field modulated magnetic properties of underoxidized SrRuO3 (SRO)/SrTiO3 (STO) heterostructures are investigated using first-principles calculations. The spin polarization of underoxidized SRO/STO heterostructures turns from negative to positive as the electric field changes from -0.2 to 0.2 V nm-1. The underoxidized SRO/STO heterostructure with 7 SRO atomic layers turns from perpendicular magnetic anisotropy to in-plane magnetic anisotropy as the electric field turns from -0.2 to 0.2 V nm-1, which can be attributed to the in-plane dx2-y2 and out-of-plane dxz, dyz orbitals. The Dzyaloshinskii-Moriya interaction of underoxidized SRO/STO heterostructures can also be effectively tailored using an electric field. These results indicate that the use of electric field is an effective method to modulate magnetic properties of perovskite-type oxide heterostructures, which is beneficial for the development of the high-performance spintronic devices.
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http://dx.doi.org/10.1039/d0cp06362bDOI Listing
February 2021

Hypergraph-based persistent cohomology (HPC) for molecular representations in drug design.

Brief Bioinform 2021 Jan 22. Epub 2021 Jan 22.

Division of Mathematical Sciences, School of Physical and Mathematical Sciences, Nanyang Technological University, 637371, Singapore.

Artificial intelligence (AI) based drug design has demonstrated great potential to fundamentally change the pharmaceutical industries. Currently, a key issue in AI-based drug design is efficient transferable molecular descriptors or fingerprints. Here, we present hypergraph-based molecular topological representation, hypergraph-based (weighted) persistent cohomology (HPC/HWPC) and HPC/HWPC-based molecular fingerprints for machine learning models in drug design. Molecular structures and their atomic interactions are highly complicated and pose great challenges for efficient mathematical representations. We develop the first hypergraph-based topological framework to characterize detailed molecular structures and interactions at atomic level. Inspired by the elegant path complex model, hypergraph-based embedded homology and persistent homology have been proposed recently. Based on them, we construct HPC/HWPC, and use them to generate molecular descriptors for learning models in protein-ligand binding affinity prediction, one of the key step in drug design. Our models are tested on three most commonly-used databases, including PDBbind-v2007, PDBbind-v2013 and PDBbind-v2016, and outperform all existing machine learning models with traditional molecular descriptors. Our HPC/HWPC models have demonstrated great potential in AI-based drug design.
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http://dx.doi.org/10.1093/bib/bbaa411DOI Listing
January 2021

Development and validation of a novel score to predict dialysis inadequacy in continuous ambulatory peritoneal dialysis patients.

J Int Med Res 2021 Jan;49(1):300060520984591

Division of Nephrology, Department of Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan, China.

Objective: Adequate dialysis is of great importance for continuous ambulatory peritoneal dialysis (CAPD) patients. This study aimed to develop and validate an easily applicable quantitative dialysis adequacy risk scoring system in CAPD patients based on laboratory parameters from a single blood draw.

Methods: A total of 634 CAPD patients from four study centers were enrolled in this study (345 and 289 patients in development and validation groups, respectively). A risk score model for inadequate dialysis was developed based on multivariate regression analysis, which was validated by the area under the receiver operator curve and calibrated by a calibration curve.

Results: Seven independent predictors for inadequate dialysis were identified in the development group (male sex, hypoalbuminemia, anemia, being overweight, hyperuricemia, estimated glomerular filtration rate <4.7 mL/min/1.73 m, and serum creatinine >800 μmol/L). A risk prediction score model was established and validated in the development and validation groups. Further analysis indicated that this model is suitable for CAPD patients with a wide range of clinical manifestations.

Conclusion: An easily applicable novel risk scoring system was established to detect inadequate dialysis in CAPD patients.
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http://dx.doi.org/10.1177/0300060520984591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829543PMC
January 2021

Removal of acetaminophen through direct electron transfer by reactive MnO: Efficiency, mechanism and pathway.

Sci Total Environ 2020 Dec 26;769:144377. Epub 2020 Dec 26.

Department of Environmental Science and Engineering, Hubei Environmental Remediation Material Engineering Technology Research Center, School of Resource and Environmental Sciences, Wuhan University, Wuhan 430079, China. Electronic address:

MnO with certain oxidative reactivity, was fabricated via sol-gel method and applied for the removal of acetaminophen (APAP). The mechanism of APAP oxidation was revealed in depth through electrochemical tests and X-ray photoelectron spectroscopy (XPS). Moreover, the selective abatement of various organic contaminants contained different functional groups by MnO was investigated through linear free energy relationship (LFER) estimated with peak potentials (E) of these organic contaminants. Under acidic condition, APAP could be effectively eliminated by MnO. The open circuit potential (OCP) and zeta potential tests illustrate that the oxidative reactivity of MnO is associated to the surface acid-base behavior of MnO and its surface charge situation. The XPS experiments and Mn leaching results imply that Mn(III) could capture electron from APAP and release Mn to aqueous phase. The intermediates could be ascribed to fragmentation of acetamido radicals and phenoxy radicals, both of which were formed through electron transfer from APAP to MnO. The reactive MnO shows selective oxidation of different contaminants in the electron transfer process. LFER analysis indicates good negative linear correlation between lnk and E of various pollutants. The efficiency of MnO in the elimination of APAP and selective oxidation of different contaminants suggest some new insights for transformation of APAP and other electron-rich pollutants in the environments.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144377DOI Listing
December 2020

Multiple 'omics'-analysis reveals the role of prostaglandin E2 in Hirschsprung's disease.

Free Radic Biol Med 2021 Feb 16;164:390-398. Epub 2021 Jan 16.

State Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166, China. Electronic address:

The etiology and pathogenesis of Hirschsprung's disease (HSCR) remain largely unknown. We examined colon tissues from three independent populations with a combined analysis of metabolomics, transcriptomics and proteomics to understand HSCR pathogenesis, according to which mouse model was used to examine prostaglandin E2 (PGE2) induced clinical presentation of HSCR. SH-SY5Y and SK-N-BE(2) cell lines were studied for PGE2 inhibited cell migration through EP2. Our integrated multiple 'omics'-analysis suggests that the levels of PGE2, the expression of the gene encoding PGE2 receptor (EP2), and PGE2 synthesis enzyme genes (PTGS1 and PTGES) increased in HSCR colon tissues, together with a decreased synthesis of PGE2-related byproducts. In vivo, the pregnant mice treated with PGE2 gave birth to offspring with the decrease of ganglion cells in their colon and gut function. In in vitro study, when EP2 was blocked, the PGE2-inhibited cell migration was recovered. Our study identified a novel pathway highlighting the link between expression of PTGS1 and PTGES, levels of PGE2, expression of PTGER2, and neural crest cell migration in HSCR, providing a novel strategy for future diagnosis and prevention of HSCR.
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http://dx.doi.org/10.1016/j.freeradbiomed.2020.12.456DOI Listing
February 2021

Quantitative evaluation of chronically obstructed kidneys from noncontrast computed tomography based on deep learning.

Eur J Radiol 2021 Mar 10;136:109535. Epub 2021 Jan 10.

Department of Radiology, Peking University First Hospital, Peking University, 8 XiShiKu Street, Beijing 100034, People's Republic of China. Electronic address:

Objective: To quantitatively report renal parenchymal volume (RPV), renal sinus volume (RSV), and renal parenchymal density (RPD) for chronically obstructed kidneys from noncontrast computed tomography (NCCT).

Methods: This retrospective study was approved by the institutional review board of our hospital with a waiver of informed consent. We retrospectively collected 304 consecutive NCCT scans of urinary obstruction and constructed two datasets: one with 167 patient scans for parenchyma and sinus segmentation (segmentation dataset) and the other containing 137 scans from different patients diagnosed with chronic urinary obstruction (CUO dataset) and paired with split glomerular filtration rate (sGFR). A cascaded three-dimensional (3D) U-Net model was developed and validated for parenchyma and sinus segmentation. The RPV, RSV, and RPD of the CUO dataset were calculated by the model with manual editing. A multivariate analysis was performed to show the association between all parameters and the sGFR.

Results: In the test dataset, the Dice values for parenchyma and sinus segmentation were 0.95 ± 0.04 and 0.90 ± 0.05, respectively. Compared with those of nonobstructed kidneys, the RSV and RPD of obstructed kidneys increased, but RPV and sGFR decreased (P < .001). For chronically obstructed kidneys, age (r = -0.292, P < .001), RPV (r = 0.849, P < .001), RSV (r = -0.331, P < .001), and RPD (r = -0.296, P < .001) were significantly correlated with sGFR. The fitted regression model was sGFR = 10.873-0.111 Age + 0.211 RPV - 0.022 RSV (r = 0.712).

Conclusions: NCCT combined with deep learning has the potential to be a single radiological procedure for morphological and functional evaluation of chronically obstructed kidneys.
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http://dx.doi.org/10.1016/j.ejrad.2021.109535DOI Listing
March 2021

The effect of hydrodynamics on the succession of autotrophic and heterotrophic organisms of biofilms in river ecosystems.

Water Sci Technol 2021 Jan;83(1):63-76

College of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224003, Jiangsu Province, China E-mail:

Biofilms were cultivated for a 68-day period under different hydrodynamic conditions, and the effect of hydrodynamics on the succession of autotrophic and heterotrophic organisms of biofilms was investigated. Five obvious stages were observed during biofilm formation. At Stage I, the attachment of algae was delayed, especially under turbulent conditions. After Stage II, algal density and heterotrophic biomass of biofilms increased, which were obvious under turbulent flow. Therefore, the algal density and heterotrophic biomass of biofilms were largest under turbulent condition, followed by laminar condition, and then transitional condition. Diatoms were dominant in all flumes and were most abundant under turbulent conditions. The proportion of cyanobacteria was highest under laminar conditions. The ratio of aerobic to anaerobic bacteria decreased and their co-existence could facilitate the nitrification and denitrification in the biofilm. The ratio of monounsaturated fatty acids to saturated fatty acids was highest under turbulent conditions on the 15th day. While the ratio was highest under laminar condition on the 48th day, the high ratio indicates the high ability of biofilm to obtain nutrients, which affect the growth of algae. The regulation of hydrodynamics is a useful technology which can affect the growth of the microorganisms of biofilm, and further improve water quality.
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http://dx.doi.org/10.2166/wst.2020.536DOI Listing
January 2021

The complete chloroplast genome of (Berberidaceae), a narrowly distributed plant species in China.

Mitochondrial DNA B Resour 2020 Jul 2;5(3):2631-2633. Epub 2020 Jul 2.

Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, China.

L. is the largest herbaceous genus of Berberidaceae which comprises more than 60 species. is a rare plant species only narrowly inhabited in the Puding County in the Guizhou Province of China. Here, we first report the complete chloroplast genome of assembled from Illumina short-read sequencing data. The chloroplast genome of was 157,325 bp in length, with a total GC content of 36.11%. A total of 112 unique genes were identified, among which are 78 protein-coding genes, 30 tRNA genes and four rRNA genes. The phylogenetic analysis revealed that closely related to . Our study will provide useful fundamental data for further phylogenetic and evolutionary studies of the genus.
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http://dx.doi.org/10.1080/23802359.2020.1781560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782884PMC
July 2020

The complete chloroplast genome sequence of L.

Mitochondrial DNA B Resour 2020 May 22;5(3):2139-2140. Epub 2020 May 22.

Chongqing Institute of Traditional Chinese Medicine, Chongqing, China.

is a traditional natural plant pharmaceutical with antimicrobial, antioxidant, and antidiarrheal effects and mainly distributed in China and Southeast Asian countries. The complete chloroplast sequence of has been determined in this study. The cpDNA was 158,981 bp in length, containing a pair of inverted repeats of 30,872 bp each separated by a large and small single-copy region of 84,347 and 12,890 bp, respectively. The genome contained 86 protein-coding genes, eight rRNA genes, and 37 tRNA genes. The overall GC content of the chloroplast genome was 38%. Phylogenetic tree demonstrated that closely related to and
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http://dx.doi.org/10.1080/23802359.2019.1693931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782515PMC
May 2020

The complete chloroplast genome of B. L. Guo et Hsiao (Berberidaceae).

Mitochondrial DNA B Resour 2020 May 12;5(3):2045-2046. Epub 2020 May 12.

School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, China.

The genus of belongs to Berberidaceae family, which is famous for their medicinal and ornamental value. In recent years, has attracted increasing attention due to their medicinal and nutritive value. In this research, we reported the complete chloroplast (cp) genome of The complete chloroplast of this species is 157,076 bp in length, including a pair of invert repeat regions (IRS) (25,833 bp) that is divided by a large single copy area (LSC) (88,340 bp) and a small single copy area (SSC) (17,070 bp). The circular chloroplast genome of . contains 112 unique genes, composing of 78 protein-coding genes, 30 tRNA, and four rRNA genes. Phylogenetic analysis indicates that . has a closer relationship with .
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http://dx.doi.org/10.1080/23802359.2020.1730269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782069PMC
May 2020