Publications by authors named "Xiang Li"

5,396 Publications

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Au-FeO heterodimer multifunctional nanoparticles-based platform for ultrasensitive naked-eye detection of Salmonella typhimurium.

J Hazard Mater 2022 May 13;436:129140. Epub 2022 May 13.

College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, PR China. Electronic address:

In this work, we developed an ultrasensitive colorimetry for Salmonella typhimurium detection with multifunctional Au-FeO dumbbell-like nanoparticles (DBNPs) which possessed easy bio-modifiability, excellent LSPR characteristics, superparamagnetic properties and super peroxidase-like activity. In the detection, the anti-S. typhimurium antibody modified DBNPs (IDBNPs) bound with S. typhimurium and aggregated on their surfaces in a large number, which showed much quicker magnetic response than free IDBNPs. By controlling appropriate separation conditions, [email protected] typhimurium composites were captured, while free IDBNPs were remained in the supernatant. Therefore, by detecting the absorbance of the supernatant, quantitative detection was achieved from 10 to 1000 CFU/mL. Moreover, utilizing the peroxidase-like activity of IDBNPs, we further realized semi-quantitative naked-eye detection. By adding ABTS into the above supernatant, which was oxidized to green chelate (OxABTS), colorimetric signal was amplified significantly, and meanwhile, the green chelates and the wine-red IDBNPs engendered mixed color, enhancing the range of color gradation and greatly improving the visual resolution. Finally, a detection limit (10 CFU/mL) comparable with that of above spectrum measurement was achieved. Besides, our method exhibited efficient capture capability (nearly 100% even for rare S. typhimurium), and had good stability and specificity, and acceptable anti-interference ability in fetal bovine serum and milk samples.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129140DOI Listing
May 2022

Thioredoxin 1 regulates the pentose phosphate pathway via ATM phosphorylation after experimental subarachnoid hemorrhage in rats.

Brain Res Bull 2022 May 16;185:162-173. Epub 2022 May 16.

Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China; Institute of Stroke Research, Soochow University, Suzhou 215006, China. Electronic address:

Subarachnoid hemorrhage (SAH), a type of hemorrhagic stroke, is a neurological emergency with high morbidity and mortality. Early brain injury (EBI) after SAH is the leading cause of poor prognosis in SAH patients. TRX system is a NADPH-dependent antioxidant system which is composed of thioredoxin reductase (TRXR), thioredoxin (TRX). The pentose phosphate pathway (PPP), a pathway through which glucose can be metabolized, is a major source of NADPH. Thioredoxin 1 (TRX1) is a member of thioredoxin system mainly located in cytoplasm. Serine/threonine kinases ataxia telangiectasia mutated (ATM) is an important oxidative stress receptor, and TRX1 can regulate ATM phosphorylation and then affect the activity of PPP key enzyme glucose 6-phosphate dehydrogenase (G6PD). However, whether TRX1 is involved in the regulation of PPP pathway after subarachnoid hemorrhage remains unclear. The results showed that after SAH, the level of TRX1 and phosphor-ATM decreased while the level of TRXR1 increased. G6PD protein level remained unchanged but the activity decreased, and the NADPH contents decreased. Overexpression of TRX1 by lentivirus upregulates the level of phosphor-ATM, G6PD activity and NADPH content. TRX1 overexpression improved short-term and long-term neurobehavioral outcomes and alleviated neuronal impairment in rats. Nissl staining showed that upregulation of TRX1 reduced cortical neuron injury. Our study shows that TRX1 participates in the PPP pathway by regulating phosphorylation ATM, which is accomplished by affecting G6PD activity. TRX1 may be an important target for EBI intervention after SAH.
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http://dx.doi.org/10.1016/j.brainresbull.2022.05.008DOI Listing
May 2022

Dangling Octahedra Enable Edge States in 2D Lead Halide Perovskites.

Adv Mater 2022 May 18:e2201666. Epub 2022 May 18.

School of Materials Science and Engineering, Tianjin Key Laboratory of Metal and Molecule-Based Material Chemistry, Nankai University, Tianjin, 300350, China.

The structural reconstruction at the crystal layer edges of 2D lead halide perovskites (LHPs) leads to unique edge states (ES) which are manifested by prolonged carrier lifetime and reduced emission energy. These special ES could effectively enhance the optoelectronic performance of devices, but their intrinsic origin and working mechanism remain elusive. Here we demonstrate that the ES of a family of 2D Ruddlesden-Popper LHPs [BA CsPb Br , BA MAPb Br and BA MA Pb Br (BA = butylammonium; MA = methylammonium)] arise from the rotational symmetry elevation of the PbBr octahedra dangling at the crystal layer edges. These dangling octahedra give rise to localized electronic states that enable an effective transport of electrons from the interior to layer edges, and the population of electrons in both the intrinsic states and ES can be manipulated via controlling the external fields. Moreover, the abundant phonons, activated by the dangling octahedra, can interact with electrons to facilitate radiative recombination, counterintuitive to the suppressive role commonly observed in conventional semiconductors. This work unveils the intrinsic atomistic and electronic origins of ES in 2D LHPs, which could stimulate the exploration of ES-based exotic optoelectronic properties and corresponding design of high-performance devices for these emergent low-dimensional semiconductors. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202201666DOI Listing
May 2022

Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon α2b.

J Pharm Anal 2022 Apr 8;12(2):308-316. Epub 2021 Jul 8.

National Institutes for Food and Drug Control, Beijing, 100050, China.

Recombinant human interferon α2b (rhIFNα2b) is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C. The current identification test for rhIFNα2b is complex. In this study, an anti-rhIFNα2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNα2b. RhIFNα2b was used to immunize an alpaca, which established a phage nanobody library. After five steps of enrichment, the nanobody I22, which specifically bound rhIFNα2b, was isolated and inserted into the prokaryotic expression vector pET28a. After subsequent purification, the physicochemical properties of the nanobody were determined. A semiquantitative detection and rapid identification assay of rhIFNα2b was developed using this novel nanobody. To develop a rapid test, the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips. The developed rhIFNα2b detection assay had a limit of detection of 1 μg/mL. The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products. The principle of this novel assay is generally applicable for the rapid testing of other commercial products, with a great potential for routine use in detecting counterfeit recombinant protein products.
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http://dx.doi.org/10.1016/j.jpha.2021.07.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091920PMC
April 2022

Advancements on Small Molecule PROTACs Containing an Indole/Spiro-fused Oxoindole Scaffolds: An Emerging Degrader Targeting Cancers.

Anticancer Agents Med Chem 2022 May 9. Epub 2022 May 9.

State Key Laboratory of Southwestern Chinese Medicine Resources, Hospital of Chengdu University of Traditional Chinese Medicine, School of Pharmacy and College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

Indole and spiro-fused oxoindole frameworks widely exist in a variety of natural bioactive products, pharmaceuticals and drug candidates, featuring unique functions in the regulation of proliferation, infiltration, and metastasis of cancer cells. In recent years, significant progress of proteolysis targeting chimeric (PROTAC) technology that employs ubiquitin-proteasome system (UPS) to eliminate disease-associated proteins has been witnessed, thus opening a promising avenue to the discovery of new indole-related drugs. In this review, we focus on summarizing the achievements on small molecule PROTACs that involve indole/spiro-fused oxoindole scaffolds in the part of ligands of the protein of interest (POI). Current challenges and future directions in this promising field are discussed at the end of this review. For the convenience of readers, our review is divided into five parts according to the types of target proteins. We hope this review could bring a quick look and some inspiration to researchers in relevant fields.
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http://dx.doi.org/10.2174/1871520622666220509175305DOI Listing
May 2022

Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis.

Chin Med 2022 May 17;17(1):57. Epub 2022 May 17.

College of Acupuncture, Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, Sichuan, China.

Background: Myocardial fibrosis changes the structure of myocardium, leads to cardiac dysfunction and induces arrhythmia and cardiac ischemia, threatening patients' lives. Electroacupuncture at PC6 (Neiguan) was previously found to inhibit myocardial fibrosis. Long non-coding RNAs (lncRNAs) play a variety of regulatory functions in myocardial fibrosis, but whether electroacupuncture can inhibit myocardial fibrosis by regulating lncRNA has rarely been reported.

Methods: In this study, we constructed myocardial fibrosis rat models using isoproterenol (ISO) and treated rats with electroacupuncture at PC6 point and non-point as control. Hematoxylin-eosin, Masson and Sirius Red staining were performed to assess the pathological changes and collagen deposition. The expression of fibrosis-related markers in rat myocardial tissue were detected by RT-qPCR and Western blot. Miat, an important long non-coding RNA, was selected to study the regulation of myocardial fibrosis by electroacupuncture at the transcriptional and post-transcriptional levels. In post-transcriptional level, we explored the myocardial fibrosis regulation effect of Miat on the sponge effect of miR-133a-3p. At the transcriptional level, we studied the formation of heterodimer PPARG-RXRA complex and promotion of the TGF-β1 transcription.

Results: Miat was overexpressed by ISO injection in rats. We found that Miat can play a dual regulatory role in myocardial fibrosis. Miat can sponge miR-133a-3p in an Ago2-dependent manner, reduce the binding of miR-133a-3p target to the 3'UTR region of CTGF mRNA and improve the protein expression level of CTGF. In addition, it can also directly bind with PPARG protein, inhibit the formation of heterodimer PPARG-RXRA complex and then promote the transcription of TGF-β1. Electroacupuncture at PC6 point, but not at non-points, can reduce the expression of Miat, thus inhibiting the expression of CTGF and TGF-β1 and inhibiting myocardial fibrosis.

Conclusion: We revealed that electroacupuncture at PC6 point can inhibit the process of myocardial fibrosis by reducing the expression of lncRNA Miat, which is a potential therapeutic method for myocardial fibrosis.
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http://dx.doi.org/10.1186/s13020-022-00615-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112552PMC
May 2022

Multifunctional metal-organic framework-based nanoreactor for starvation/oxidation improved indoleamine 2,3-dioxygenase-blockade tumor immunotherapy.

Nat Commun 2022 May 16;13(1):2688. Epub 2022 May 16.

Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.

Inhibited immune response and low levels of delivery restrict starvation cancer therapy efficacy. Here, we report on the co-delivery of glucose oxidase (GOx) and indoleamine 2,3-dioxygenase (IDO) inhibitor 1-methyltryptophan using a metal-organic framework (MOF)-based nanoreactor, showing an amplified release for tumor starvation/oxidation immunotherapy. The nanosystem significantly overcomes the biobarriers associated with tumor penetration and improves the cargo bioavailability owing to the weakly acidic tumor microenvironment-activated charge reversal and size reduction strategy. The nanosystem rapidly disassembles and releases cargoes in response to the intracellular reactive oxygen species (ROS). GOx competitively consumes glucose and generates ROS, further inducing the self-amplifiable MOF disassembly and drug release. The starvation/oxidation combined IDO-blockade immunotherapy not only strengthens the immune response and stimulates the immune memory through the GOx-activated tumor starvation and recruitment of effector T cells, but also effectively relieves the immune tolerance by IDO blocking, remarkably inhibiting the tumor growth and metastasis in vivo.
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http://dx.doi.org/10.1038/s41467-022-30436-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110376PMC
May 2022

CLEC14A was up-regulated in hepatocellular carcinoma and may function as a potential diagnostic biomarker.

Clinics (Sao Paulo) 2022 13;77:100029. Epub 2022 May 13.

Chongqing University Three Gorges Hospital, Chongqing, China. Electronic address:

Objective: The current work aimed to investigate the expression and potential clinical significance of C-type Lectin domain family 14 (CLEC14A) in hepatocellular carcinoma.

Methods: The relative expressions of CLEC14A in the Hepatocellular Carcinoma (HCC) tissue and adjacent normal tissue of 105 HCC patients were examined using RT-qPCR methods. Furthermore, Receiver Operating Characteristic (ROC) curve was drawn for exploring the diagnostic value of CLEC14A. Next, the expressions of CLEC14A in HCC cell lines and normal liver epithelial cells were compared, and the effects of knockdown of CLEC14A on the growth and apoptosis of HCC cells were examined.

Results: The authors found that the expression of CLEC14A was markedly increased in hepatocellular carcinoma tumors in comparison with the adjacent tissue, and the expression level of CLEC14A was positively correlated with the size and differentiation of the tumor. Moreover, results of ROC analysis showed CLEC14A might function as a sensitive diagnostic biomarker for HCC. Furthermore, CLEC14A was up-regulated in HCC cell lines, and transient over-expression of CLEC14A decreased the proliferation and increased the apoptosis of HCC cells in vitro.

Conclusions: Our results suggested that CLEC14A was up-regulated in HCC and might function as a potential diagnostic marker.
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http://dx.doi.org/10.1016/j.clinsp.2022.100029DOI Listing
May 2022

Glutathione-Triggered Mitochondria-Targeting Reassembly from Polymeric Micelles to Nanofibers for a Synergistic Anticancer Effect.

ACS Macro Lett 2022 Apr 1;11(4):543-548. Epub 2022 Apr 1.

CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China.

Nanofibers self-assembled from peptides have attracted much attention to inhibit cancer cells. However, there are still some disadvantages, including high concentration for self-assembly and incapability to load drugs, which limit their applications. In this report, we rationally integrate self-assembled peptides, glutathione-sensitive disulfide bonds, and mitochondrial targeting moieties into the amphiphilic block copolymer to construct the nanocarriers, which can be used to load anticancer drug doxorubicin (DOX). After cellular internalization, the nanocarriers can reassemble from micelles to nanofibers under the trigger by glutathione and locate in mitochondria. The released DOX and nanofibers induce mitochondrial dysfunction and activate the apoptosis pathway to synergistically inhibit tumor cells. This organelle-specific drug delivery system with reassembly capability from micelles to nanofibers shows great potential for effectively killing cancer cells.
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http://dx.doi.org/10.1021/acsmacrolett.2c00059DOI Listing
April 2022

Transition metal copper composite ionic liquid self-built ratiometric sensor for the detection of paracetamol.

Anal Chim Acta 2022 May 2;1209:338992. Epub 2021 Sep 2.

Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China; Institute of Engineering Biology and Health, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, China. Electronic address:

In this paper, a MOF derivative Cu-PF-MOF composed of transition metal copper and ionic liquid [BMIM] PF was used to construct a ratiometric electrochemical sensor for paracetamol detection. Cu-PF-MOF was synthesized by chemical bath method and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET), X-ray Photoelectron Spectroscopy (XPS) and X-ray powder diffraction (XRD). Owing to the introduction of ionic liquid [BMIM] PF and the synthetic effect on transition metal copper and ionic liquid [BMIM] PF, Cu-PF-MOF has the higher conductivity, larger electroactive surface area and better intrinsic catalytic properties of the skeletal transition metal, exhibiting enhanced electrocatalytic response to the reduction of paracetamol. The stable reduction peak at -104 mV was used as the ratiometric signal for analytical paracetamol detection using differential pulse voltammetry (DPV), with a linear range of 0.1-100 μM and a detection limit of 0.03 μM (S/N = 3). In addition, the constructed sensor showed good reproducibility, stability and interference resistance, as well as ideal recoveries (98.20%-104.40%) for the analysis of paracetamol in water samples.
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http://dx.doi.org/10.1016/j.aca.2021.338992DOI Listing
May 2022

Carotenoid Profile in Maternal/Cord Plasma and Changes in Breast Milk along Lactation and Its Association with Dietary Intake: A Longitudinal Study in a Coastal City in Southern China.

Nutrients 2022 May 9;14(9). Epub 2022 May 9.

Abbott Nutrition Research & Development Centre, Shanghai 200233, China.

In this study, changes of carotenoids in breast milk were observed longitudinally for up to one year. Our study aimed to analyze the profile of carotenoids in breast milk and maternal/cord plasma and its correlation with dietary intake in Guangzhou. Plasma and breast milk samples of five stages during lactation (i.e., colostrum; transitional milk; and early, medium, and late mature milk) were collected from lactating mothers. The food frequency questionnaire (FFQ) was used for collecting data on dietary intake in the corresponding stages. Levels of lutein, zeaxanthin, β-cryptoxanthin, β-carotene, and lycopene were analyzed by high-performance liquid chromatography. We found that the total carotenoid level decreased gradually with the extension of lactation and eventually stabilized. Among them, the content of lutein increased from colostrum to transitional milk and decreased thereafter until it plateaued in the mature milk. Furthermore, lutein was reported as the dominant nutrient in maternal plasma, cord plasma, transitional milk, and mature milk at up to 400 days postpartum, while beta-carotene was predominant in colostrum. The content of β-carotenoid in middle and late mature breast milk was related to dietary intake (r = 1.690, < 0.05). Carotenoid level in cord blood was lower than that in the mother's plasma and was related to the carotenoid intake in the mother's diet. Correlation of carotenoids between maternal and umbilical cord blood, breast milk, and maternal blood could well reflect the transport of carotenoids. These findings may help to guide mothers' diets during breastfeeding.
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http://dx.doi.org/10.3390/nu14091989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100325PMC
May 2022

Transcriptome Revealed Exposure to the Environmental Ammonia Induced Oxidative Stress and Inflammatory Injury in Spleen of Fattening Pigs.

Animals (Basel) 2022 May 7;12(9). Epub 2022 May 7.

College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.

Ammonia is one of the major environmental pollutants that seriously threaten human health. Although many studies have shown that ammonia causes oxidative stress and inflammation in spleen tissue, the mechanism of action is still unclear. In this study, the ammonia poisoning model of fattening pigs was successfully established. We examined the morphological changes and antioxidant functions of fattening pig spleen after 30-day exposure to ammonia. Effects of ammonia in the fattening pig spleen were analyzed from the perspective of oxidative stress, inflammation, and histone methylation via transcriptome sequencing technology (RNA-seq) and real-time quantitative PCR validation (qRT-PCR). We obtained 340 differential expression genes (DEGs) by RNA-seq. Compared with the control group, 244 genes were significantly upregulated, and 96 genes were significantly downregulated in the ammonia gas group. Some genes in Gene Ontology (GO) terms were verified and showed significant differences by qRT-PCR. The KEGG pathway revealed significant changes in the MAPK signaling pathway, which is strongly associated with inflammatory injury. To sum up, the results indicated that ammonia induces oxidative stress in pig spleen, activates the MAPK signaling pathway, and causes spleen necrosis and injury. In addition, some differential genes encoding epigenetic factors were found, which may be involved in the response mechanism of spleen tissue oxidative damage. The present study provides a transcriptome database of ammonia-induced spleen poisoning, providing a reference for risk assessment and comparative medicine of ammonia.
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http://dx.doi.org/10.3390/ani12091204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101760PMC
May 2022

Stability, aggregation, and sedimentation behaviors of typical nano metal oxide particles in aqueous environment.

J Environ Manage 2022 May 10;316:115217. Epub 2022 May 10.

School of Environmental and Engineering, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

The wide use of nano metal oxide particles (NMOPs) brings about their inevitable release into the water environment, affecting the environment and human health. Therefore, the stability, aggregation, and sedimentation process of four typical NMOPs (ZnO NPs, CeO NPs, TiO NPs, and CuO NPs) were investigated in artificial water and real municipal sewage to reveal their complicated behavior. Results showed that NMOPs aggregated at the pH of zero-charge point, and their hydrodynamic diameters and aggregation rates could reach the maximum values. The hydrodynamic diameters and aggregation rates of ZnO NPs, CeO NPs, TiO NPs, and CuO NPs at the zero-charge point were 617, 1760, 870, 1502 nm, and 31.7, 1158.1, 48.3, 115.7 nm/min, respectively. In addition, the dissolution of NMOPs led to the sedimentation rates under acidic conditions being much lower than those under neutral and alkaline conditions. The aggregation and sedimentation performance of NMOPs were affected by not only pH but also ionic strength (IS) and species. The aggregation rates of NMOPs increased with the increase of IS (0-10 mM), and the maximum aggregation rate of CeO NPs was 470.1 nm/min (pH = 7 and CaCl = 10 mM). According to Coulomb's law, divalent cations (Mg, Ca) were more competitively adsorbed on the surface of NMOPs than monovalent cations (K, Na), which increased the zeta potential and aggregation rate of NMOPs. Furthermore, the NMOPs were easier to aggregate in municipal sewage because of the homogeneous aggregation between nanoparticles and heterogeneous aggregation with natural colloids. The total interaction energy between NMOPs was calculated by the classical Derjaguin-Landau-Verwey-Overbeek (DLVO) theoretical formula, which was consistent with the experimental results.
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http://dx.doi.org/10.1016/j.jenvman.2022.115217DOI Listing
May 2022

Gamma knife radiosurgery for trigeminal schwannomas: A systematic review and meta-analysis.

J Clin Neurosci 2022 May 10;101:67-79. Epub 2022 May 10.

Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China. Electronic address:

Background: Gamma knife radiosurgery (GKRS) has been deemed as the gold standard stereotactic radiosurgery (SRS) mode for the treatment of intracranial tumors, cerebrovascular diseases and brain functional diseases. Our study was aimed to systematically evaluate the efficacy, safety, and complications of gamma knife radiosurgery for trigeminal schwannomas.

Method: We performed a systematic review and meta-analysis to analyze the clinical outcomes of patients with trigeminal schwannomas treated primarily or adjunctly with GKRS. We searched two databases, Pubmed and Embase, for studies published before January 1, 2021, using GKRS for trigeminal schwannomas. Studies reporting treatment of other schwannomas, or other forms of SRS such as linear accelerator and Cyberknife were excluded to reduce its heterogeneity.

Results: Our search achieved 351 studies, of which 35 were assessed for full-text eligibility. 19 studies were included in the meta-analysis. 456 of 504 patients (0.94, 95% CI 0.91-0.96, I = 3.02%, p < 0.01) from 18 studies had local control, and 278 of 489 patients (0.63, 95%CI 0.48-0.78, I = 88.75%, p < 0.01) from 16 studies experienced tumor regression or disappearance. 231 of 499 patients (0.50, 95% CI 0.37-0.62; I = 83.89%, P < 0.01) from 17 studies had clinical symptoms improved. There was no significant difference in tumor control between those treated with GKRS as either primary treatment or adjuvant to surgery(p = 0.390).

Conclusion: GKRS is an efficacious primary and adjuvant method of treating trigeminal schwannomas, with reliable tumor control rates. Randomized controlled trials are needed to further and comprehensively evaluate the benefit-to-risk ratio of gamma knife radiosurgery.
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http://dx.doi.org/10.1016/j.jocn.2022.05.001DOI Listing
May 2022

Vesicular Ganglioside GM1 From Breast Tumor Cells Stimulated Epithelial-to-Mesenchymal Transition of Recipient MCF-10A Cells.

Front Oncol 2022 26;12:837930. Epub 2022 Apr 26.

School of Medicine, Northwest University, Xi'an, China.

Small extracellular vesicles (sEVs) are a type of membrane structure secreted by cells, which are involved in physiological and pathological processes by participating in intercellular communication. Glycosphingolipids (GSLs) are enriched in sEV and can be delivered to recipient cells. In this study, we found that overexpression of B3GALT4, the glycosyltransferase responsible for ganglioside GM1 synthesis, can induce the epithelial-mesenchymal transition (EMT) process in MCF-10A cells. Moreover, GM1 was verified to be presented on sEV from breast cancer cells. Overexpression of B3GALT4 resulted in elevated vesicular GM1 levels and increased sEV secretion in breast cancer cells. Proteomic analysis revealed that eleven sEV secretion-related proteins were differentially expressed, which might contribute to the altered sEV secretion. Of the identified proteins, 15 oncogenic differentially expressed proteins were documented to be presented in sEV. With the treatment of GM1-enriched sEV from breast cancer cells, the EMT process was induced in recipient non-tumorigenic epithelial MCF-10A cells. Our findings demonstrated that GM1-enriched sEVs derived from breast cancer cells induced the EMT process of recipient cells, which might provide essential information on the biological function of vesicular GM1.
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http://dx.doi.org/10.3389/fonc.2022.837930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086854PMC
April 2022

On the relation between crowding and ensemble perception: Examining the role of attention.

Psych J 2022 May 12. Epub 2022 May 12.

School of Psychology, Jiangxi Normal University, Nanchang, China.

Ensemble perception of a crowd of stimuli is very accurate, even when individual stimuli are invisible due to crowding. The ability of high-precision ensemble perception can be an evolved compensatory mechanism for the limited attentional resolution caused by crowding. Thus the relationship of crowding and ensemble coding is like two sides of the same coin wherein attention may play a critical factor for their coexistence. The present study investigated whether crowding and ensemble coding were similarly modulated by attention, which can promote our understanding of their relation. Experiment 1 showed that diverting attention away from the target harmed the performance in both crowding and ensemble perception tasks regardless of stimulus density, but crowding was more severely harmed. Experiment 2 showed that directing attention toward the target bar enhanced the performance of crowding regardless of stimulus density. Ensemble perception of high-density bars was also enhanced but to a lesser extent, while ensemble perception of low-density bars was harmed. Together, our results indicate that crowding is strongly modulated by attention, whereas ensemble perception is only moderately modulated by attention, which conforms to the adaptive view.
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http://dx.doi.org/10.1002/pchj.559DOI Listing
May 2022

The Application of Neural Network Technology Based on Mea-Bp Algorithm in the Prediction of Microdosimetric Qualities.

Radiat Prot Dosimetry 2022 May 12. Epub 2022 May 12.

School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, PR China.

The most abundant products of the interaction between radiation and matter are low-energy electrons, and the collisions between these electrons and biomolecules are the main initial source of radiation-based biological damage. To facilitate the rapid and accurate quantification of low-energy electrons (0.1-10 keV) in liquid water at different site diameters (1-2000 nm), this study obtained ${\overline{y}}_{\mathrm{F}}$ and ${\overline{y}}_{\mathrm{D}}$data for low-energy electrons under these conditions. This paper proposes a back-propagation (BP) neural network optimized by the mind evolutionary algorithm (MEA) to construct a prediction model and evaluate the corresponding prediction effect. The results show that the ${\overline{y}}_{\mathrm{F}}$ and ${\overline{y}}_{\mathrm{D}}$ values predicted by the MEA-BP neural network algorithm reach a training precision on the order of ${10}^{-8}$. The relative error range between the prediction results of the validated model and the Monte Carlo calculation results is 0.03-5.98% (the error range for single-energy electrons is 0.1-5.98%, and that for spectral distribution electrons is 0.03-4.4%).
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http://dx.doi.org/10.1093/rpd/ncac062DOI Listing
May 2022

Overexpression of MCAM induced by SMYD2-H3K36me2 in breast cancer stem cell properties.

Breast Cancer 2022 May 12. Epub 2022 May 12.

Department of Medical Oncology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, No. 44, Xiaoheyan Road, Dadong District, Shenyang, 110042, Liaoning, People's Republic of China.

Background: Melanoma cell adhesion molecule (MCAM) is highly expressed in various malignancies. However, studies on the effects of MCAM on stemness of cancer stem cells are limited. Here, we aimed to explore the relationship between MCAM and stem cell phenotype in breast cancer (BC).

Methods: We analyzed the genes differentially expressed in BC from the oncomine database, followed by TCGA-BRCA database validation. We then used gene set enrichment analysis to analyze the signaling pathways enriched to the relevant genes, followed by loss-of-function experiments to analyze the role of MCAM in the growth of BC cells and the maintenance of stem cell properties. We analyzed the cause for the MCAM overexpression using ChIP-seq and clarified the upstream mechanism by constructing SE-Deleted cells. Finally, the role of SMYD2 in the growth of BC cells and the maintenance of stem cell properties were verified by rescue experiments.

Results: MCAM was significantly overexpressed in BC, which predicted somber prognosis in patients. Knockdown of MCAM drastically hindered the growth and metastasis of BC cells in vitro and in vivo. Subsequently, the MCAM promoter was observed to have significant H3K36me2 modification and that SMYD2 could significantly promote the expression of MCAM. In addition, further overexpression of SMYD2 in cells with MCAM knockdown increased MCAM expression and promoted the growth as well as stemness of BC cells.

Conclusion: SMYD2 can elevate the expression of MCAM by promoting its H3K36me2 modification, which in turn expedites the growth and stem cell properties of BC cells.
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http://dx.doi.org/10.1007/s12282-022-01365-xDOI Listing
May 2022

Screening and identification of key microRNAs and regulatory pathways associated with the renal fibrosis process.

Mol Omics 2022 May 13. Epub 2022 May 13.

Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China.

Renal fibrosis (RF) is a chronic and fatal disease related to the gradual deterioration of kidney function. MicroRNAs (miRNAs) play a key role in cellular functions and several of them related to the pathogenesis of RF have been identified, although the underlying mechanisms are unclear. In order to explore the miRNAs involved in RF progression, we established a model in rats by the unilateral ureteral ligation method. The animals were randomly divided into the control group, and the 2 week, 4 week and 6 week model groups. The indices of renal function were measured using routine biochemical assays. The differentially expressed miRNAs (DE-miRNAs) between the sham-operated and modelled rats were screened, and their putative target genes were identified using the miRanda software and functionally annotated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The expression of transforming growth factor β1 (TGF-β1), Smad3 and Smad7 was confirmed by RT-PCR. Compared to the sham-operated group, the model groups showed a decrease in SOD activity, along with the increased renal coefficient, and higher MDA, HYP, Scr, BUN and ALB levels. In addition, TGF-β1, Smad3 and Smad7 were also upregulated in the RF groups. We identified 274 known and 11 novel DE-miRNAs in the 2 week, 114 known and 6 novel DE-miRNAs in the 4 week, and 41 known and 1 novel DE-miRNAs in the 6 week model groups. The putative target genes of these DE-miRNAs were enriched in metabolic processes, apoptosis, pyrimidine metabolism, and TNF and VEGF signalling pathways. Based on our findings, we surmise that miR-146a-3p, miR-148a-3p, miR-130a-5p, miR-362-3p and miR-122-5p are likely to be involved in the occurrence and development of RF, and miR-122-5p may play an inhibitory role. The underlying mechanisms need further investigation.
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http://dx.doi.org/10.1039/d1mo00498kDOI Listing
May 2022

Developing a novel Bifidobacterium phage quantitative polymerase chain reaction-based assay for tracking untreated wastewater.

Sci Total Environ 2022 May 9:155815. Epub 2022 May 9.

School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China; Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China. Electronic address:

Microbial source tracking (MST) tools provide insights on fecal pollution levels in aquatic environments using predominantly quantitative PCR (qPCR) assays that target host-associated fecal marker genes. Existing wastewater-associated marker genes have shown limited or significant cross-reactions with non-human fecal samples. In this study, we mined the current Gut Phage Database (GPD) and designed a novel untreated wastewater-specific Bifidobacterium phage qPCR assay (i.e., Bifi assay). The sensitivity and specificity of the Bifi marker genes were assessed by collectively analyzing untreated (n = 33) and treated (n = 15) wastewater and non-human fecal samples (i.e., Rabbit, mouse, cow, horse, pig, chicken, sheep, dog, deer, kangaroos; n = 113) in Shenzhen, Guangdong Province, China and Brisbane, Australia. Bifi assay revealed 100% host-specificity against non-human fecal samples collected from Shenzhen and Brisbane. Furthermore, this marker gene was also detected in all untreated and treated wastewater samples, whose concentrations ranged from 5.54 to 6.83 log GC/L. In Shenzhen, the concentrations of Bifi marker gene were approximately two orders of magnitude lower than Bacteroides (HF183/BacR287 assay) and CrAssphage (CPQ_56 assay). The concentration of Bifi marker gene in untreated wastewater from Brisbane was 1.35 log greater than those in Shenzhen. Our results suggest that Bifi marker gene has the potential to detect and quantify the levels of human fecal pollution in Shenzhen and Brisbane. If additional detection sensitivity is required for environmental studies, Bifi marker gene should be paired with either CrAssphage or HF183/BacR287.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155815DOI Listing
May 2022

Early prediction of moderate-to-severe condition of inhalation-induced acute respiratory distress syndrome via interpretable machine learning.

BMC Pulm Med 2022 May 12;22(1):193. Epub 2022 May 12.

College of Pulmonary and Critical Care Medicine, Chinese People's Liberation Army General Hospital, Beijing, 100853, China.

Background: Several studies have investigated the correlation between physiological parameters and the risk of acute respiratory distress syndrome (ARDS), in addition, etiology-associated heterogeneity in ARDS has become an emerging topic quite recently; however, the intersection between the two, which is early prediction of target conditions in etiology-specific ARDS, has not been well-studied. We aimed to develop and validate a machine-learning model for the early prediction of moderate-to-severe condition of inhalation-induced ARDS.

Methods: Clinical expertise was applied with data-driven analysis. Using data from electronic intensive care units (retrospective derivation cohort) and the three most accessible vital signs (i.e. heart rate, temperature, and respiratory rate) together with feature engineering, we applied a random forest approach during the time window of 90 h that ended 6 h prior to the onset of moderate-to-severe respiratory failure (the ratio of partial pressure of arterial oxygen to fraction of inspired oxygen ≤ 200 mmHg).

Results: The trained random forest classifier was validated using two independent validation cohorts, with an area under the curve of 0.9127 (95% confidence interval 0.8713-0.9542) and 0.9026 (95% confidence interval 0.8075-1), respectively. A Stable and Interpretable RUle Set (SIRUS) was used to extract rules from the RF to provide guidelines for clinicians. We identified several predictive factors, including resp_96h_6h_min < 9, resp_96h_6h_mean ≥ 16.1, HR_96h_6h_mean ≥ 102, and temp_96h_6h_max > 100, that could be used for predicting inhalation-induced ARDS (moderate-to-severe condition) 6 h prior to onset in critical care units. ('xxx_96h_6h_min/mean/max': the minimum/mean/maximum values of the xxx vital sign collected during a 90 h time window beginning 96 h prior to the onset of ARDS and ending 6 h prior to the onset from every recorded blood gas test).

Conclusions: This newly established random forest‑based interpretable model shows good predictive ability for moderate-to-severe inhalation-induced ARDS and may assist clinicians in decision-making, as well as facilitate the enrolment of patients in prevention programmes to improve their outcomes.
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http://dx.doi.org/10.1186/s12890-022-01963-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098141PMC
May 2022

Optimized mobilization of MHC class I- and II- restricted immunity by dendritic cell vaccine potentiates cancer therapy.

Theranostics 2022 24;12(7):3488-3502. Epub 2022 Apr 24.

College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, P. R. China.

The participation of major histocompatibility complex (MHC) in antigen presentation shapes both the breadth and magnitude of specific T cell response. Dendritic cells (DCs) activated with nucleic acid or protein that encodes/incorporates multiple antigenic epitopes elicit MHC class I- and II- biased immunity, respectively. Studies demonstrate that an elevated MHC class I-directed CD8 cytotoxicity T lymphocyte (CTL) response is able to provide survival benefits to patient with malignant tumor. However, a fully effective cancer therapy must elicit a diverse repertoire of both CD4 and CD8 T cell responses, raising demands on a multifaceted activation of the MHC system. Current therapeutic strategies usually lack an orchestrated mobilization of the MHC class I and II responses. Vaccines with little synergistic effect or unmanageable elicitation of the CD4 and CD8 T cell immunity usually fail to induce a potent and durable anti-tumor protection. Here, cationic nanoemulsions (CNEs) complexed with full-length tumor model antigen ovalbumin (OVA) in the form of mRNA or protein were constructed and used as two antigenic platforms to prepare DCs vaccines with tailored MHC participation (i.e., mRNA-DCs and protein-DCs). In exploring a vaccine regimen with optimal tumor suppressing effect, the mixing ratio of mRNA-DCs and protein-DCs was manipulated. Therapeutic DCs vaccines involving both antigenic platforms induced better anti-tumor immunity in murine E.G7-OVA lymphoma model and B16-OVA melanoma model, which can be further augmented upon a meticulous reallocation of the MHC class I and II responses. This work indicated that a simultaneous and coordinated mobilization of the MHC-restricted immunity might potentiate cancer therapy.
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http://dx.doi.org/10.7150/thno.71760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065178PMC
May 2022

Roles of Negatively Charged Histone Lysine Acylations in Regulating Nucleosome Structure and Dynamics.

Front Mol Biosci 2022 25;9:899013. Epub 2022 Apr 25.

Greater Bay Biomedical InnoCenter, Shenzhen Bay Laboratory (SZBL), Shenzhen, China.

The nucleosome, the basic repeating unit of chromatin, is a dynamic structure that consists of DNA and histones. Insights derived from biochemical and biophysical approaches have revealed that histones posttranslational modifications (PTMs) are key regulators of nucleosome structure and dynamics. Mounting evidence suggests that the newly identified negatively charged histone lysine acylations play significant roles in altering nucleosome and chromatin dynamics, subsequently affecting downstream DNA-templated processes including gene transcription and DNA damage repair. Here, we present an overview of the dynamic changes of nucleosome and chromatin structures in response to negatively charged histone lysine acylations, including lysine malonylation, lysine succinylation, and lysine glutarylation.
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http://dx.doi.org/10.3389/fmolb.2022.899013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081332PMC
April 2022

Intracellular Activity of Poly (DL-lactide-co-glycolide) Nanoparticles Encapsulated with Prothionamide, Pyrazinamide, Levofloxacin, Linezolid or Ethambutol on Multidrug-Resistant Mycobacterium Tuberculosis.

Curr Drug Deliv 2022 May 11. Epub 2022 May 11.

School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China.

Background: Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) is a major cause of death amongst tuberculosis patients. Nanomedicine avoids some limitations of conventional drug treatment and increases therapeutic efficacy against bacterial infections. However, the effect of anti-TB drug nanoparticle (NP) compounds in anti-TB regimens against MDR-TB remains unclear.

Objective: The objective of this article is to prepare levofloxacin, linezolid, ethambutol, prothionamide, and pyrazinamide encapsulated NPs and to evaluate their therapeutic efficacy against MDR-TB in macrophages.

Methods: Drug-loaded PLGA NPs were prepared by the multiple emulsion method. The colocalization, intracellular release, and anti-TB activity of these NPs were investigated on cultured macrophages. The immune phenotype of the macrophages, including their mitochondrial membrane potential, reactive oxygen species (ROS), and nitric oxide (NO) production, was evaluated following treatment with NPs or free drug compounds.

Results: All drug-loaded PLGA NPs were spherical in shape, 150 to 210 nm in size, and showed 14.22% to 43.51% encapsulation efficiencies and long-duration release. Drug-loaded PLGA NPs were mainly distributed in the cytoplasm of macrophages, showed high cellular compatibility, and maintained their concentration for at least 13 days. Compared with the free drug compounds, the number of colonies after exposure to PLGA NP compounds was significantly less. The enhanced antibacterial activity of the NP compounds may be due to the enhanced levels of ROS and NO and the increased early apoptosis stress within M. tuberculosis-infected macrophages additionally.

Conclusion: The application of PLGA NP compounds not only enhances drug efficacy but also induces innate bactericidal events in macrophages, confirming this as a promising approach for MDR-TB therapy.
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http://dx.doi.org/10.2174/1567201819666220511120215DOI Listing
May 2022

[Warming acupuncture combined with "three steps and seven methods" of for chronic nonspecific low back pain of deficiency and cold-dampness blockage: a randomized controlled trial].

Zhongguo Zhen Jiu 2022 May;42(5):505-10

Department of Tuina, Rehabilitation Hospital Affiliated to Fujian University of TCM, Fuzhou 350003, China; Fujian Provincial Key Laboratory of Rehabilitation Technology, Fuzhou 350003.

Objective: To compare the clinical efficacy and possible mechanism of warming acupuncture combined with "three steps and seven methods" of and simple "three steps and seven methods" of in treatment of chronic nonspecific low back pain (NLBP) of deficiency and cold-dampness blockage.

Methods: A total of 138 patients were randomized into an observation group (69 cases, 5 cases dropped off) and a control group (69 cases, 7 cases dropped off). In the control group, "three steps and seven methods" of was applied. On the basis of the treatment in the control group, warming acupuncture was applied at Shenshu (BL 23), Yaoyangguan (GV 3), Mingmen (GV 4), Weizhong (BL 40) and points. The treatment was given once a day, 6 times a week for 3 weeks in both groups. Before and after treatment, the short form of McGill pain questionnaire (SF-MPQ) score, Oswestry disability index (ODI) score, finger-to-floor distance (FFD), Schober test distance, fear-avoidance beliefs questionnaire (FABQ) score and deficiency and cold-dampness blockage score were observed, the serum levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and thromboxane B (TXB) were detected in both groups. The recurrence rate was evaluated in follow-up of 6 months after treatment.

Results: After treatment, the scores of PRI, PPI, VAS, ODI, FABQ and FFD, deficiency and cold-dampness blockage scores were decreased compared before treatment in both groups (<0.01), and those in the observation group were lower than the control group (<0.01); the Schober test distances were increased compared before treatment in both groups (<0.01), and that in the observation group was larger than the control group (<0.01). After treatment, the serum levels of TNF-α, IL-1β, IL-6 and TXB were decreased compared before treatment in both groups (<0.01), and those in the observation group were lower than the control group (<0.01). In follow-up, the recurrence rate was 12.8% (6/47) in the observation group, which was lower than 34.3% (12/35) in the control group (<0.05).

Conclusion: Warming acupuncture combined with "three steps and seven methods" of can effectively alleviate pain in patients with chronic NLBP of deficiency and cold-dampness blockage, improve activity and dysfunction of waist, the clinical efficacy is superior to simple "three steps and seven methods" of , its mechanism may be relate to the inhibition of inflammatory reaction.
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http://dx.doi.org/10.13703/j.0255-2930.20210622-k0001DOI Listing
May 2022

The Aryl Sulfide Synthesis via Sulfide Transfer.

Chemistry 2022 May 11:e202200869. Epub 2022 May 11.

College of Materials Science and Opto-Electronic Technology &, Center of Materials Science and Optoelectronics Engineering &, CAS Center for Excellence in Topological Quantum Computation &, CAS Key Laboratory of Vacuum Physic, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.

Aryl sulfides are in great demands in drugs and materials sciences. To avoid using nucleophilic and noxious thiols, many efforts have been focused on exploring novel sulfide resources. Herein, a reductive Pd-catalyzed, Ni-mediated method to synthesize aryl sulfides via a sulfide transfer reaction is developed. The utility and scope of this reaction is exemplified by various aryl electrophiles and aryl sulfides. Mechanistic studies reveal two competing catalytic cycles of sulfide transfer and aryl transfer in this reaction, where the former one is favored over the later one because of the large energy barrier difference during the transmetalation. Moreover, two important chemicals are late-stage functionalized by this method, exhibiting the potential applications in drugs and materials science.
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http://dx.doi.org/10.1002/chem.202200869DOI Listing
May 2022

Effectiveness of containment strategies in preventing SARS-CoV-2 transmission.

J Infect Public Health 2022 Apr 29;15(6):609-614. Epub 2022 Apr 29.

Department of Microbiology and Immunology, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates. Electronic address:

Background: Despite substantial resources deployed to curb SARS-CoV-2 transmission, controlling the COVID-19 pandemic has been a major challenge. New variants of the virus are frequently emerging leading to new waves of infection and re-introduction of control measures. In this study, we assessed the effectiveness of containment strategies implemented in the early phase of the pandemic.

Methods: Real-world data for COVID-19 cases was retrieved for the period Jan 1 to May 1, 2020 from a number of different sources, including PubMed, MEDLINE, Facebook, Epidemic Forecasting and Google Mobility Reports. We analyzed data for 18 countries/regions that deployed containment strategies such as travel restrictions, lockdowns, stay-at-home requests, school/public events closure, social distancing, and exposure history information management (digital contact tracing, DCT). Primary outcome measure was the change in the number of new cases over 30 days before and after deployment of a control measure. We also compared the effectiveness of centralized versus decentralized DCT. Time series data for COVID-19 were analyzed using Mann-Kendall (M-K) trend tests to investigate the impact of these measures on changes in the number of new cases. The rate of change in the number of new cases was compared using M-K z-values and Sen's slope.

Results: In spite of the widespread implementation of conventional strategies such as lockdowns, travel restrictions, social distancing, school closures, and stay-at-home requests, analysis revealed that these measures could not prevent the spread of the virus. However, countries which adopted DCT with centralized data storage were more likely to contain the spread.

Conclusions: Centralized DCT was more effective in containing the spread of COVID-19. Early implementation of centralized DCT should be considered in future outbreaks. However, challenges such as public acceptance, data security and privacy concerns will need to be addressed.
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http://dx.doi.org/10.1016/j.jiph.2022.04.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052634PMC
April 2022

Effectiveness and safety of acupuncture for angina pectoris: An overview of systematic reviews.

Integr Med Res 2022 Sep 6;11(3):100864. Epub 2022 Apr 6.

Acupuncture and Tuina School The 3rd Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.

Background: The number of systematic reviews meta-analyses (SRs/MAs) on the effectiveness of acupuncture for angina pectoris (AP) is increasing. Due to the inconsistent conclusions and unknown quality of these SRs/MAs, this overview aimed to systematically evaluate and synthesize the existing SRs/MAs, attempting to provide more reliable evidence for the effectiveness and safety of acupuncture in the treatment of AP.

Methods: SRs/MAs were searched via eight databases from inception to March 14, 2022. The risk of bias was evaluated using the Risk of Bias in Systematic reviews (ROBIS) tool. The quality of the methodology, reporting, and evidence were assessed by the Assess Systematic Reviews 2 (AMSTAR-2), the Preferred Reporting Item for Systematic Review and Meta-analysis for Acupuncture (PRISMA-A), and the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) system, respectively.

Results: Sixteen SRs/MAs were included and fifteen SRs/MAs were considered being of critically low quality according to AMSTAR-2. Only three SRs/MAs were rated at low risk of bias. No study reported all the items listed in the PRISMA-A checklist. No high-quality evidence with GRADE assessment was found. With the low-quality evidence, acupuncture combined with other interventions was superior to monotherapy (medications or Chinese medicine) in the angina symptom and electrocardiogram recovery. No adverse effects owing to acupuncture were reported.

Conclusions: Owing to the lack of high-quality evidence provided by the current SRs/MAs, the effectiveness of acupuncture for AP still warrants further proof. Further researches with more critical design and methodology are needed for providing more convincing evidence.

Registration: This review was registered at PROSPERO (www.crd.york.ac.uk/prospero/): CRD42021219367.
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http://dx.doi.org/10.1016/j.imr.2022.100864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077518PMC
September 2022

Ultrasound-Guided Quadratus Lumborum Block Enhances the Quality of Recovery after Gastrointestinal Surgery: A Randomized Controlled Trial.

Pain Res Manag 2022 30;2022:8994297. Epub 2022 Apr 30.

Department of Anesthesiology, Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, China.

Background: Quadratus lumborum block (QLB) has been used to reduce postoperative acute pain and opioid consumption. However, the efficacy of QLB on the quality of recovery (QoR) after gastrointestinal surgery has not been established. The aim of this study was to evaluate the ability of QLB to enhance the postoperative QoR in patients undergoing open gastrointestinal surgery.

Methods: Eighty-four patients undergoing open gastrointestinal surgery were randomized to receive ultrasound-guided QLB with either 20 ml of 0.375% ropivacaine or saline. The primary outcome was the QoR-15 score at 24 h after surgery. The secondary outcomes were the postoperative pain intensity, opioid consumption, the incidence of nausea, vomiting, and chronic pain.

Results: The global QoR-15 score at 24 h postoperatively was significantly higher in the QLB group than in the control group (mean difference: 16.9; 95% CI: 11.9-21.9). Additionally, the QoR-15 scores for five dimensions were significantly higher in the QLB group than in the control group. The cumulative oxycodone consumption was significantly lower in the QLB group during 0-6, 6-24, 0-24, 24-48, and 0-48 h postoperatively than in the control group. At rest or during coughing, the pain verbal rating scale scores were significantly lower at 1, 3, 6, 12, and 24 h after surgery in the QLB group than in the control group. The incidence of postoperative nausea was significantly different between the groups, but postoperative vomiting was not.

Conclusion: Single-injection posteromedial QLB with ropivacaine enhanced the QoR at 48 h after surgery and improved analgesia during the early postoperative period in patients undergoing gastrointestinal surgery.
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http://dx.doi.org/10.1155/2022/8994297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078840PMC
May 2022

Highly stretchable, elastic, antimicrobial conductive hydrogels with environment-adaptive adhesive property for health monitoring.

J Colloid Interface Sci 2022 Apr 26;622:612-624. Epub 2022 Apr 26.

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada. Electronic address:

Hypothesis: Development of soft conductive materials has enabled the promising future of wearable electronics for motion sensing. However, conventional soft conductive materials typically lack robust adhesive and on-demand removable properties for a target substrate. Therefore, it is believed that the integration of superior mechanical properties, electrical conductivity, and tunable adhesive properties into hydrogels would support and improve their reliable sensing performance.

Experiments: A hydrogel ionic conductor composed of cationic micelles crosslinked in the polyacrylamide (PAM) network was designed and fabricated. The viscoelastic, mechanical, adhesion, electrical, and antimicrobial properties of the hydrogel were systematically characterized.

Findings: The developed ionic conductor possesses a range of desirable properties including mechanical performances such as excellent stretchability (>1100%), toughness, elasticity (recovery from 1000% strain), conductivity (2.72 S·m), and antimicrobial property, owing to the multiple non-covalent supramolecular interactions (e.g., hydrogen bonding, hydrophobic, and π-π/cation-π interactions) present in the cross-linked network. Meanwhile, the developed hydrogel is incorporated with different stimuli-responsive polymers and exhibits a tunable adhesive property (triggerable attachment and on-demand removable capabilities) in adapt to the surrounding environmental conditions (i.e., pH, temperature). With all these significant features, the resulting hydrogel ionic conductor serves as a proof-of-concept motion-sensing system with excellent sensitivity and enhanced reliability for the detection of a wide range of motions.
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http://dx.doi.org/10.1016/j.jcis.2022.04.119DOI Listing
April 2022
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