Publications by authors named "Xiang Gao"

2,248 Publications

  • Page 1 of 1

Cumulative Consumption of Sulfur Amino Acids and Risk of Diabetes: a Prospective Cohort Study.

J Nutr 2022 Aug 9. Epub 2022 Aug 9.

Orentreich Foundation for the Advancement of Science, Inc., Cold Spring, NY.

Background: Cross-sectional studies suggested that consumption of sulfur amino acids (SAAs) including methionine and cysteine is associated with a higher risk of type 2 diabetes (T2D) in humans and T2D-related biomarkers in animals. But, whether higher long-term SAA intake increases the risk of T2D in humans remains unknown.

Objectives: We aimed to investigate the association between long-term dietary SAA intake and risk of T2D.

Methods: We analyzed data collected from two different cohorts of the Framingham Heart Study, a long-term, prospective, and ongoing study. The Offspring cohort (1991-2014) included participants from fifth through ninth exam, and Third-Generation cohort (2002-2011) included participants from first and second exam. After excluding participants with a clinical history of diabetes, missing dietary data or implausible total energy intake, 3,222 participants in the Offspring and 3,205 participants in the Third-Generation cohort were included. Dietary intake was assessed using a validated food frequency questionnaire. The relations between energy-adjusted total SAA (methionine and cysteine) intake or individual SAA intake (in quintiles) and risk of incident T2D were estimated via Cox proportional hazards models after adjusting for dietary and non-dietary risk factors. Associations across the two cohorts were determined by direct combination and meta-analysis.

Results: During the 23 years follow-up, 472 participants reported a new diagnosis of T2D in the two cohorts. In the meta-analysis, the Hazard Ratios (95% Confidence Interval) of T2D comparing the highest with the lowest intake of total SAAs, methionine, and cysteine were 1.8 (1.3, 2.5), 1.7 (1.2, 2.3), and 1.4 (1.0, 2.1), respectively. The association of SAA intake with T2D was attenuated after adjusting animal protein intake in sensitivity analyses.

Conclusions: Our findings show that excess intake of SAA is associated with higher risk of T2D. Dietary patterns that are low in SAA diet may help in preventing T2D.
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http://dx.doi.org/10.1093/jn/nxac172DOI Listing
August 2022

Artificial Intelligence Model for Antiinterference Cataract Automatic Diagnosis: A Diagnostic Accuracy Study.

Front Cell Dev Biol 2022 22;10:906042. Epub 2022 Jul 22.

Health Management Institute, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, China.

Cataract is the leading cause of blindness worldwide. In order to achieve large-scale cataract screening and remarkable performance, several studies have applied artificial intelligence (AI) to cataract detection based on fundus images. However, the fundus images they used are original from normal optical circumstances, which is less impractical due to the existence of poor-quality fundus images for inappropriate optical conditions in actual scenarios. Furthermore, these poor-quality images are easily mistaken as cataracts because both show fuzzy imaging characteristics, which may decline the performance of cataract detection. Therefore, we aimed to develop and validate an antiinterference AI model for rapid and efficient diagnosis based on fundus images. The datasets (including both cataract and noncataract labels) were derived from the Chinese PLA general hospital. The antiinterference AI model consisted of two AI submodules, a quality recognition model for cataract labeling and a convolutional neural networks-based model for cataract classification. The quality recognition model was performed to distinguish poor-quality images from normal-quality images and further generate the pseudo labels related to image quality for noncataract. Through this, the original binary-class label (cataract and noncataract) was adjusted to three categories (cataract, noncataract with normal-quality images, and noncataract with poor-quality images), which could be used to guide the model to distinguish cataract from suspected cataract fundus images. In the cataract classification stage, the convolutional-neural-network-based model was proposed to classify cataracts based on the label of the previous stage. The performance of the model was internally validated and externally tested in real-world settings, and the evaluation indicators included area under the receiver operating curve (AUC), accuracy (ACC), sensitivity (SEN), and specificity (SPE). In the internal and external validation, the antiinterference AI model showed robust performance in cataract diagnosis (three classifications with AUCs >91%, ACCs >84%, SENs >71%, and SPEs >89%). Compared with the model that was trained on the binary-class label, the antiinterference cataract model improved its performance by 10%. We proposed an efficient antiinterference AI model for cataract diagnosis, which could achieve accurate cataract screening even with the interference of poor-quality images and help the government formulate a more accurate aid policy.
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http://dx.doi.org/10.3389/fcell.2022.906042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355278PMC
July 2022

The biochemical and molecular investigation of flower color and scent sheds lights on further genetic modification of ornamental traits in .

Hortic Res 2022 17;9:uhac114. Epub 2022 May 17.

Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun 130024, China.

is renowned for its evergreen and strap-like leaves, whereas its floral color and scent are lacking diversity. Here, anthocyanin, volatile terpene, and carotenoid metabolisms were integrally investigated in flowers. The results showed that pelargonidins and lutein might cooperate to confer orange or yellow color to flowers, but only a trace amount of (+)-limonene was detected. The expression levels of and appeared to be responsible for the ratio of cyanidin and pelargonidin derivatives in , and the low expression of was responsible for the lack of cyanidins in flowers. Moreover, the promoter could not be activated by CmMYBAs, suggesting that it was controlled by novel regulators. Only two CmTPSs were functional, with CmTPS2 responsible for (+)-limonene synthesis, contributing to the monotonous flower volatile terpenes of . CmCCD1a and CmCCD1b were able to cleave carotenoids at the 5,6 (5',6'), and 9,10 (9',10') positions to generate volatile apocarotenoids, whereas the substrates found in low-quantities or specific subcellular localizations of CmCCD1s might constrain volatile apocarotenoid release. Consequently, activating and introducing novel or versatile may be effective ways to modify the floral color and scent, respectively. Alternatively, modifying the carotenoid flux or CCD1 localization might affect floral color and scent simultaneously. Taking these results together, the present study provides a preliminary deciphering of the genetic constraints underlying flower color and scent development, and proposes possible schemes for further genetic modification of ornamental traits in and other plants.
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http://dx.doi.org/10.1093/hr/uhac114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343915PMC
May 2022

Cavernous sinus dural arteriovenous fistula complicated by hippocampus venous congestion: A case report.

J Stroke Cerebrovasc Dis 2022 Jul 27;31(9):106666. Epub 2022 Jul 27.

Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, Zhejiang 315010, China. Electronic address:

Background: Hippocampal venous congestion is a rare complication associated with cavernous sinus dural arteriovenous fistulas (CS-DAVFs).

Case Description: A 74-year-old woman was admitted to the hospital with a swollen left eye. Isolated lesions were found in the left hippocampus and the middle cerebellar peduncle. Cerebral angiography revealed retrograde venous drainage of the bilateral inferior petrosal sinuses from the left CS-DAVF. The patient underwent transcatheter arterial embolization, resulting in complete resolution of the hippocampal lesions and neurological symptoms.

Conclusion: Hippocampal injury is a rare complication of CS-DAVF. Attentive diagnosis and treatment can effectively prevent adverse consequences.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2022.106666DOI Listing
July 2022

DNA Methylation of Patatin-Like Phospholipase Domain-Containing Protein 6 Gene Contributes to the Risk of Intracranial Aneurysm in Males.

Front Aging Neurosci 2022 11;14:885680. Epub 2022 Jul 11.

Department of Neurosurgery, Ningbo First Hospital, Ningbo, China.

: This study is aimed to investigate the contribution of patatin-like phospholipase domain-containing protein 6 () DNA methylation to the risk of intracranial aneurysm (IA) in the Han Chinese population. : A total of 96 age- and sex-matched participants were recruited to evaluate methylation bisulfite pyrosequencing. The mRNA expression in the plasma was determined using real-time quantitative reverse transcription-polymerase chain reaction. Human primary artery smooth muscle cells (HPCASMC) were used for the in vitro function study. : methylation was significantly higher in patients with IA than in healthy controls ( < 0.01). Sex group analysis showed that this correlation appeared in the male group ( < 0.01) but not in the female group ( > 0.05). methylation was significantly associated with age in all participants ( = 0.306, = 0.003) and in the control group ( = 0.377, = 0.008) but not in the IA group ( = 0.127, = 0.402). Furthermore, the mRNA expression significantly decreased in patients with IA than that in the controls ( = 0.016). expression was significantly inversely correlated with elevated DNA methylation in participants (r = -0.825, < 0.0001). In addition, transcription was significantly enhanced following treatment with 5-aza-2'-deoxycytidine methylation inhibitor in HPCASMC.The receiver operating characteristic analyses of curves showed that the mean methylation [area under the curve (AUC) = 0.74, < 0.001] and mRNA expression (AUC = 0.86, < 0.001) could have a diagnostic value for patients with IA. : Although future functional experiments are required to test our hypothesis, our study demonstrated that methylation and mRNA expression were significantly associated with the risk of IA; thus, they show potential for use in the early diagnosis of IA.
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http://dx.doi.org/10.3389/fnagi.2022.885680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309567PMC
July 2022

A Novel Necroptosis-Related Prognostic Signature of Glioblastoma Based on Transcriptomics Analysis and Single Cell Sequencing Analysis.

Brain Sci 2022 Jul 26;12(8). Epub 2022 Jul 26.

Department of Neurosurgery, Ningbo First Hospital, Zhejiang University School of Medicine, Ningbo 315010, China.

Background: Glioblastoma (GBM) is the most common and deadly brain tumor. The clinical significance of necroptosis (NCPS) genes in GBM is unclear. The goal of this study is to reveal the potential prognostic NCPS genes associated with GBM, elucidate their functions, and establish an effective prognostic model for GBM patients.

Methods: Firstly, the NCPS genes in GBM were identified by single-cell analysis of the GSE182109 dataset in the GEO database and weighted co-expression network analysis (WGCNA) of The Cancer Genome Atlas (TCGA) data. Three machine learning algorithms (Lasso, SVM-RFE, Boruta) combined with COX regression were used to build prognostic models. The subsequent analysis included survival, immune microenvironments, and mutations. Finally, the clinical significance of NCPS in GBM was explored by constructing nomograms.

Results: We constructed a GBM prognostic model composed of NCPS-related genes, including CTSD, AP1S1, YWHAG, and IER3, which were validated to have good performance. According to the above prognostic model, GBM patients in the TCGA and CGGA groups could be divided into two groups according to NCPS, with significant differences in survival analysis between the two groups and a markedly worse prognostic status in the high NCPS group ( <0.001). In addition, the high NCPS group had higher levels of immune checkpoint-related gene expression, suggesting that they may be more likely to benefit from immunotherapy.

Conclusions: Four genes (CTSD, AP1S1, YWHAG, and IER3) were screened through three machine learning algorithms to construct a prognostic model for GBM. These key and novel diagnostic markers may become new targets for diagnosing and treating patients with GBM.
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http://dx.doi.org/10.3390/brainsci12080988DOI Listing
July 2022

Ergonomics Design and Assistance Strategy of A-Suit.

Micromachines (Basel) 2022 Jul 15;13(7). Epub 2022 Jul 15.

Wuhan Second Ship Design and Research Institute, Wuhan 430205, China.

Concerning the biomechanics and energy consumption of the lower limbs, a soft exoskeleton for the powered plantar flexion of the ankle, named A-Suit, was developed to improve walking endurance in the lower limbs and reduce metabolic consumption. The method of ergonomics design was used based on the biological structures of the lower limbs. A profile of auxiliary forces was constructed according to the biological force of the Achilles tendon, and an iterative learning control was applied to shadow this auxiliary profile by iteratively modifying the traction displacements of drive units. During the evaluation of the performance experiments, four subjects wore the A-Suit and walked on a treadmill at different speeds and over different inclines. Average heart rate was taken as the evaluation index of metabolic consumption. When subjects walked at a moderate speed of 1.25 m/s, the average heart rate under the Power-ON condition was 7.25 ± 1.32% (mean ± SEM) and 14.40 ± 2.63% less than the condition of No-suit and Power-OFF. Meanwhile, the additional mass of A-Suit led to a maximum increase of 7.83 ± 1.44%. The overall reduction in with Power-ON over the different inclines was 6.93 ± 1.84% and 13.4 ± 1.93% compared with that of the No-Suit and Power-OFF condition. This analysis offers interesting insights into the viability of using this technology for human augmentation and assistance for medical and other purposes.
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http://dx.doi.org/10.3390/mi13071114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316755PMC
July 2022

Puerarin Attenuates Oxidative Stress and Ferroptosis via AMPK/PGC1α/Nrf2 Pathway after Subarachnoid Hemorrhage in Rats.

Antioxidants (Basel) 2022 Jun 27;11(7). Epub 2022 Jun 27.

Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo 315010, China.

Puerarin was shown to exert anti-oxidative and anti-ferroptosis effects in multiple diseases. The goal of this study was to explore the neuroprotective effect of puerarin on early brain injury (EBI) after subarachnoid hemorrhage (SAH) in rats. A total of 177 adult male Sprague Dawley rats were used. SAH was included via endovascular perforation. Intranasal puerarin or intracerebroventricular dorsomorphin (AMPK inhibitor) and SR18292 (PGC1α inhibitor) were administered. The protein levels of pAMPK, PGC1α, Nrf2, 4HNE, HO1, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere were significantly increased, whereas SOD, GPX4, and GSH were decreased at 24 h after SAH. Moreover, puerarin treatment significantly increased the protein levels of pAMPK, PGC1α, Nrf2, HO1, SOD, GPX4, and GSH, but decreased the levels of 4HNE, MDA, ACSL4, GSSG, and iron concentration in the ipsilateral hemisphere at 24 h after SAH. Dorsomorphin or SR18292 partially abolished the beneficial effects of puerarin exerted on neurological dysfunction, oxidative stress injury, and ferroptosis. In conclusion, puerarin improved neurobehavioral impairments and attenuated oxidative-stress-induced brain ferroptosis after SAH in rats. The neuroprotection acted through the activation of the AMPK/PGC1α/Nrf2-signaling pathway. Thus, puerarin may serve as new therapeutics against EBI in SAH patients.
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http://dx.doi.org/10.3390/antiox11071259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312059PMC
June 2022

Polydopamine-based nanozyme with dual-recognition strategy-driven fluorescence-colorimetric dual-mode platform for Listeria monocytogenes detection.

J Hazard Mater 2022 Jul 12;439:129582. Epub 2022 Jul 12.

Research Unit of Food Safety, Chinese Academy of Medical Sciences (No. 2019RU014), NHC Key Lab of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment (CFSA), Beijing 100022, China. Electronic address:

Development of a simple and efficient dual-mode analytical technique with the built-in cross reference correction feature is benefit to achieve the highly accurate detection of the target pollutants and avoid the false-positive outputs in environmental media. Here, we synthesized a Fe-doped polydopamine ([email protected])-based nanozyme with prominent peroxide-mimetic enzyme activity and high fluorescence emission ability. On this basis, we designed a dual-recognition strategy-driven fluorescence-colorimetric dual-mode detection platform, consisting of Listeria monocytogenes (L. monocytogenes) recognition aptamer-modified [email protected] (apt/[email protected]) and vancomycin-functionalized FeO (van/FeO), for L. monocytogenes. Owing to van/FeO-powered magnetic separation, there was a L. monocytogenes concentration-dependent fluorescence enhancement of apt/[email protected] for performing fluorescence assay in the precipitate. In this case, the prominent peroxide-mimetic enzyme activity of the residual apt/[email protected] in the precipitation could catalyze HO to further oxidate colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue oxTMB, which displayed a L. monocytogenes concentration-dependent absorbance enhancement for carrying out colorimetric assay as well. As a result, a fluorescence-colorimetric dual-mode analytical platform was proposed to successfully detect the residual L. monocytogenes in real environmental media with acceptable results. This work showed the great prospects by integrating dual-recognition strategy into fluorescence nanozyme to develop efficient and reliable dual-mode analytical platforms for safeguarding environmental health.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129582DOI Listing
July 2022

Conjugated polymers based on metalla-aromatic building blocks.

Proc Natl Acad Sci U S A 2022 Jul 13;119(29):e2203701119. Epub 2022 Jul 13.

State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials (iChEM), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Conjugated polymers usually require strategies to expand the range of wavelengths absorbed and increase solubility. Developing effective strategies to enhance both properties remains challenging. Herein, we report syntheses of conjugated polymers based on a family of metalla-aromatic building blocks via a polymerization method involving consecutive carbyne shuttling processes. The involvement of metal orbitals in aromatic systems efficiently reduces band gaps and enriches the electron transition pathways of the chromogenic repeat unit. These enable metalla-aromatic conjugated polymers to exhibit broad and strong ultraviolet-visible (UV-Vis) absorption bands. Bulky ligands on the metal suppress π-π stacking of polymer chains and thus increase solubility. These conjugated polymers show robust stability toward light, heat, water, and air. Kinetic studies using NMR experiments and UV-Vis spectroscopy, coupled with the isolation of well-defined model oligomers, revealed the polymerization mechanism.
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http://dx.doi.org/10.1073/pnas.2203701119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303910PMC
July 2022

Cardiac-specific Trim44 knockout in rat attenuates isoproterenol-induced cardiac remodeling via inhibition of AKT/mTOR pathway.

Dis Model Mech 2022 Jul 20. Epub 2022 Jul 20.

Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China (NHC), Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

When pathological hypertrophy progresses to heart failure (HF), the prognosis is often very poor. Therefore, it is crucial to find new and effective intervention targets. Here, myocardium-specific Trim44 knockout rat was generated using CRISPR-Cas9 technology. Cardiac phenotypic observations revealed that Trim44 knockout affects cardiac morphology in baseline. Meanwhile, rats with Trim44 deficiency exhibited resistance to cardiac pathological changes in response to stimulation via ISO treatment, including the improvement of cardiac remodeling and dysfunction reflecting by morphological and functional observation, myocardial fibrosis and expression of molecular marker of cardiac stress. Furthermore, signal transduction validation associated with growth and hypertrophy development in vivo and in vitro demonstrated that Trim44 deficiency inhibited the activation of signaling pathways involved in myocardial hypertrophy, especially response to pathological stress. In conclusion, the present study indicates that Trim44 knockout exhibited attenuated role in ISO-induced pathological cardiac remodeling through blocking AKT/mTOR/GSK3β/P70S6K signaling pathway. This is the first study to demonstrate the function and importance of Trim44 on heart under baseline and pathological stress. Trim44 could be a novel therapeutic target for prevention of cardiac hypertrophy and HF.
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http://dx.doi.org/10.1242/dmm.049444DOI Listing
July 2022

Associates of Perceived Quality of Life in Chinese Older Adults Living with Cognitive Impairment.

J Gerontol Soc Work 2022 Jul 19:1-13. Epub 2022 Jul 19.

University Library, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

The aim of this study is to examine perceived quality of life in Chinese older adults living with cognitive impairment and explore its associations with caregivers' characteristics. Questionnaires were administered in person to 271 caregiver-care recipient dyads from urban communities in mainland China in 2019. We used the 40-item Alzheimer's Disease-related Quality of Life tool and asked caregiver respondents to indicate care recipients' life conditions. The questionnaire asked caregivers about their sociodemographic characteristics, levels of informal social support, caregiver burden, and depressive symptoms. Caregivers' higher levels of caregiver burden (β = > -0.19,  < .01) and depressive symptoms (β = > -0.19,  < .01) amongst caregivers were significantly associated with lower quality of life among care recipients. Informal support from relatives and friends to caregivers did not significantly affect quality of life of care recipients. The results suggested that reducing caregivers' burden and depressive symptoms are essential to promote quality of life of care recipients. Formal support from health professionals, service organizations, and communities are urgently called to promote the wellbeing of Chinese families affected by cognitive impairment.
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http://dx.doi.org/10.1080/01634372.2022.2103865DOI Listing
July 2022

Multiple valence bands convergence and strong phonon scattering lead to high thermoelectric performance in p-type PbSe.

Nat Commun 2022 Jul 19;13(1):4179. Epub 2022 Jul 19.

School of Materials Science and Engineering, Beihang University, Beijing, 100191, China.

Thermoelectric generators enable the conversion of waste heat to electricity, which is an effective way to alleviate the global energy crisis. However, the inefficiency of thermoelectric materials is the main obstacle for realizing their widespread applications and thus developing materials with high thermoelectric performance is urgent. Here we show that multiple valence bands and strong phonon scattering can be realized simultaneously in p-type PbSe through the incorporation of AgInSe. The multiple valleys enable large weighted mobility, indicating enhanced electrical properties. Abundant nano-scale precipitates and dislocations result in strong phonon scattering and thus ultralow lattice thermal conductivity. Consequently, we achieve an exceptional ZT of ~ 1.9 at 873 K in p-type PbSe. This work demonstrates that a combination of band manipulation and microstructure engineering can be realized by tuning the composition, which is expected to be a general strategy for improving the thermoelectric performance in bulk materials.
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http://dx.doi.org/10.1038/s41467-022-31939-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296461PMC
July 2022

Biological Effects and Mechanisms of Caspases in Early Brain Injury after Subarachnoid Hemorrhage.

Oxid Med Cell Longev 2022 5;2022:3345637. Epub 2022 Jul 5.

Department of Neurosurgery, Ningbo First Hospital, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, Zhejiang 315010, China.

Caspases are an evolutionarily conserved family of proteases responsible for mediating and initiating cell death signals. In the past, the dysregulated activation of caspases was reported to play diverse but equally essential roles in neurodegenerative diseases, such as brain injury and neuroinflammatory diseases. A subarachnoid hemorrhage (SAH) is a traumatic event that is either immediately lethal or induces a high risk of stroke and neurological deficits. Currently, the prognosis of SAH after treatment is not ideal. Early brain injury (EBI) is considered one of the main factors contributing to the poor prognosis of SAH. The mechanisms of EBI are complex and associated with oxidative stress, neuroinflammation, blood-brain barrier disruption, and cell death. Based on mounting evidence, caspases are involved in neuronal apoptosis or death, endothelial cell apoptosis, and increased inflammatory cytokine-induced by apoptosis, pyroptosis, and necroptosis in the initial stages after SAH. Caspases can simultaneously mediate multiple death modes and regulate each other. Caspase inhibitors (including XIAP, VX-765, and Z-VAD-FMK) play an essential role in ameliorating EBI after SAH. In this review, we explore the related pathways mediated by caspases and their reciprocal regulation patterns after SAH. Furthermore, we focus on the extensive crosstalk of caspases as a potential area of research on therapeutic strategies for treating EBI after SAH.
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http://dx.doi.org/10.1155/2022/3345637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277153PMC
July 2022

Hsa-let-7d-5p Promotes Gastric Cancer Progression by Targeting PRDM5.

J Oncol 2022 7;2022:2700651. Epub 2022 Jul 7.

Research Center for Qinghai Healthy Development, Medical College, Qinghai University, Xining, Qinghai 810001, China.

Gastric cancer (GC) is a common malignant tumor in the digestive system and a significant health burden worldwide. In this study, we found that hsa-let-7d-5p was upregulated in GC cells, promoted GC cell proliferation, migration, and invasion, and reduced apoptosis. Moreover, we found that the expression of PRDM5 (PR domain protein 5) was downregulated in GC cells and upregulated in GC cells treated with hsa-let-7d-5p inhibitor. Further investigation showed that hsa-let-7d-5p was the target of PRDM5, and the functions of hsa-let-7d-5p on GC progression were rescued by PRDM5 overexpression in GC cells. Collectively, our findings suggested that hsa-let-7d-5p promoted the development of GC by targeting PRDM5, indicating that hsa-let-7d-5p could be a promising therapeutic molecule for the treatment of gastric cancer.
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http://dx.doi.org/10.1155/2022/2700651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283079PMC
July 2022

An anti-inflammatory and neuroprotective biomimetic nanoplatform for repairing spinal cord injury.

Bioact Mater 2022 Dec 2;18:569-582. Epub 2022 Jun 2.

Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215004, China.

Spinal cord regeneration after a spinal cord injury (SCI) remains a difficult challenge due to the complicated inflammatory microenvironment and neuronal damage at the injury sites. In this study, retinoic acid (RA) and curcumin (Cur) were co-loaded with bovine serum albumin (BSA) self-assembly to obtain [email protected]@Cur nanoparticles (NPs) for SCI treatment. Cur, as an antioxidant drug, facilitated reactive oxygen species (ROS) scavenging, and decreased the amount of inflammatory factors secreted by macrophages, while RA could enhance neurite extensions and neural differentiation. The constructed [email protected]@Cur NPs not only induced polarization of macrophages toward pro-regenerative phenotypes and markedly reduced the inflammatory response of macrophages or microglia, but also increased neurite length in PC12 cells and neuronal differentiation of bone marrow mesenchymal stem cells, improved the differentiation of neural stem cells (NSCs) into β3-tubulin neurons, and reversed the pro-astrocyte differentiation effect of inflammatory cytokines on NSCs. experiments revealed that [email protected]@Cur NPs regulated the phenotypic polarization of macrophages, inhibited the release of inflammatory mediators, promoted functional neuron regeneration and motor function, and further inhibited scar tissue formation. This study highlighted that the BSA-based biomimetic nanomaterials could be used as ROS scavengers and nerve regeneration promoters for treating SCI.
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http://dx.doi.org/10.1016/j.bioactmat.2022.05.026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256979PMC
December 2022

The effect of androstenedione supplementation on testosterone, estradiol, body composition, and lipid profile: a systematic review and meta-analysis of randomized controlled trials.

Hormones (Athens) 2022 Jul 16. Epub 2022 Jul 16.

Weifang Medical University, Shandong, 261000, China.

Objective: To date, no meta-analysis has been carried out to collect evidence from randomized placebo-controlled trials (RCTs) for the purpose of comprehensively summarizing the effect of androstenedione supplementation. Therefore, the aim of this research was to perform a systematic review and meta-analysis of all RCTs that explored the effect of androstenedione supplementation on individual hormonal, lipid, and anthropometric indices.

Methods: We searched five databases (Web of Science, SCOPUS, Embase, PubMed/MEDLINE, and Google Scholar) using a combination of medical subject headings (MeSH) and non-MeSH terms. Using the random-effects model, we summarized the outcomes as weighted mean difference (WMD) with 95% confidence interval (CI).

Results: Eight eligible articles were included in the meta-analysis. The pooled effect sizes suggested a significant effect of androstenedione supplementation on serum estradiol concentrations (WMD: 20.82 ng/ml, 95% CI: 7.25 to 34.38, p = 0.003), triglycerides (TG, WMD: -0.19 mg/dl, 95% CI: - 0.96, 0.57, p = 0.000), and high-density lipoprotein (HDL)-cholesterol (WMD: - 0.13 mg/dl, 95% CI: - 0.23 to - 0.03, p = 0.009); however, it had no effect on testosterone (WMD: 0.098 ng/ml, 95% CI: - 0.499 to 0.696, p = 0.748), body weight (WMD: 0.579 kg, 95% CI: - 4.02 to 5.17, p = 0.805), body mass index (BMI, WMD: - 0.73 kg/m, 95% CI: - 2.98, 1.50, p = 0.519), low-density lipoprotein (LDL)-cholesterol (WMD: - 0.074 mg/dl, 95% CI: - 0.37 to 0.22, p = 0.622), and total cholesterol (TC, WMD: - 0.15 mg/dl, 95% CI: - 0.49, 0.17, p = 0.198).

Conclusion: These findings indicate that androstenedione supplementation can lower TG and HDL-cholesterol and increase estradiol concentrations.
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http://dx.doi.org/10.1007/s42000-022-00385-8DOI Listing
July 2022

SENP1 protects cisplatin-induced AKI by attenuating apoptosis through regulation of HIF-1α.

Exp Cell Res 2022 Jul 15;419(1):113281. Epub 2022 Jul 15.

Department of Nephrology, TCM Institute of Kidney Disease, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, No. 528 ZhangHeng Road, Shanghai, 201203, China. Electronic address:

Background: Acute kidney injury is a clinical syndrome with both high morbidity and mortality. However, the underlying molecular mechanism of AKI is still largely unknown. The role of SENP1 in AKI is unclear, while one of its substrates, HIF-1α possesses nephroprotective effect in AKI. Herein, this study aimed to reveal the role of SENP1/HIF-1α axis in AKI by using both cell and animal models.

Methods: We investigated the effects of AKI on SENP1 expression using clinical samples, and cisplatin-induced AKI model based on mice or HK-2 cells. The influence of SENP1 knockdown or over-expression on cisplatin-induced AKI was studied in vitro and in vivo. Following the exploration of the change in HIF-1α expression brought by AKI, the synergistic effects of SENP1 knockdown and HIF-1α over-expression on AKI were examined.

Results: The results showed the up-regulation of SENP1 in clinical specimens, as well as cell and animal models. The knockdown or over-expression of SENP1 in HK-2 cells could promote or inhibit AKI through regulating cell apoptosis, respectively. Moreover, SENP1 mice suffered from much more serious AKI compared with mice in wild type group. Furthermore, we found that HIF-1α over-expression could attenuate the promoted cell apoptosis as well as AKI induced by SENP1 knockdown.

Conclusions: we showed that SENP1 provided protection for kidney in AKI via regulating cell apoptosis and through the regulation of HIF-1α. This study could benefit for the understanding of the pathogenesis of AKI and provide potential therapeutic target for AKI treatment.
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http://dx.doi.org/10.1016/j.yexcr.2022.113281DOI Listing
July 2022

Curcumin mitigates deoxynivalenol-induced intestinal epithelial barrier disruption by regulating Nrf2/p53 and NF-κB/MLCK signaling in mice.

Food Chem Toxicol 2022 Sep 8;167:113281. Epub 2022 Jul 8.

Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China. Electronic address:

Deoxynivalenol (DON) induces intestinal epithelial barrier disruption, posing a threat to the body. Curcumin (Cur) possesses pharmacological bioactivities such as antioxidant and anti-inflammatory effects that help maintain intestinal health. Here, the protective effects of Cur against DON-induced intestinal epithelial barrier disruption were explored. Cur (75 or 150 mg/kg body weight [B.W.]) alleviated DON-induced (2.4 mg/kg B.W.) inhibition of growth performance and morphological damage to intestinal epithelium in mice. Cur also significantly attenuated DON-induced intestinal epithelial barrier disruption and structural damage to the tight junctions (TJs), as assessed by ultrastructure observation, serum FITC-dextran concentrations and diamine oxidase activity. Cur mitigated the DON-induced increase in reactive oxygen species, malondialdehyde and 8-hydroxy-2'-deoxyguanosine levels; p53, cytoplasmic cytochrome c, Bax, and Bcl-2 expression; and TUNEL-positive cell rate and caspase-3 activity. It decreased the total antioxidant capacity and expression of nuclear Nrf2 and its downstream target genes. Lastly, Cur attenuated the DON-induced increase in MLCK, p-MLC, nuclear NF-κB p65 expression, and the NF-κB downstream target genes; decrease in the expression of TJs proteins (claudin-1, occludin, and zonula occludens-1 [ZO-1]); and abnormal ZO-1 distribution. Overall, Cur mitigated the DON-induced disruption of the intestinal epithelial barrier by regulating the Nrf2/p53-mediated apoptotic pathway and NF-κB/MLCK-mediated TJs pathway in mice.
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http://dx.doi.org/10.1016/j.fct.2022.113281DOI Listing
September 2022

Anti-breast cancer sinomenine derivatives via mechanisms of apoptosis induction and metastasis reduction.

J Enzyme Inhib Med Chem 2022 Dec;37(1):1870-1883

Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.

Sinomenine, a morphinane-type isoquinoline-derived alkaloid, was first isolated from stems and roots of (Miq.) in 1920. Later discovery by researchers confirmed various essential biological efficacy sinomenine exerted and . In this study, a series of 15 sinomenine/furoxan hybrid compounds were designed and synthesised in search of a TNBC drug candidate. Some of the target compounds exhibited strong antiproliferative activities against cancer cell lines, especially for TNBC cells, compared to positive controls. Among them, hybrid exerted superior cytotoxic effects on cancer cell lines with exceptionally low IC (0.82 μM) against MDA-MB-231 cells with the highest safety index score. Further studies in mechanism displayed that could induce an S phase cell cycle arrest, stimulate apoptosis in MDA-MB-231 cells, disrupt mitochondrial membrane potential and exert a genotoxic effect on DNA in cancer cells. In addition, also notably inhibited MDA-MB-231 cells in both migration, invasion and adhesion.
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http://dx.doi.org/10.1080/14756366.2022.2096020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272937PMC
December 2022

Secular Difference in Body Mass Index From 2014 to 2020 in Chinese Older Adults: A Time-Series Cross-Sectional Study.

Front Nutr 2022 21;9:923539. Epub 2022 Jun 21.

Department of Clinical Nutrition, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Body mass index (BMI) is the most widely used parameter to assess the body weight status. Both the increase of BMI (overweight and obesity) and decrease of BMI (underweight) has been associated with high risk of adverse outcome, such as stroke, disability, and even death. However, recent data on secular differences in BMI in the Chinese aged population are limited. The present study provides robust new evidence about the evolving epidemic of obesity among aged adults in China.

Objective: Evaluating secular difference in BMI in a group of Chinese older adults.

Materials And Methods: We analyzed 7 continuous survey years (2014-2020), including 50,192 Chinese aged participants (25,505 men and 24,687 women, aged 71.9 ± 6.1 years, age range: 65-99 years). Information on sex, age, height, and body weight, was collected based on medical history. Participants were classified into four groups: underweight (BMI < 18.5 kg/m), normal weight (18.5 kg/m ≤ BMI < 25 kg/m), overweight (25 kg/m ≤ BMI < 30 kg/m), and obesity (BMI ≥ 30 kg/m). Linear regressions were used to assess the secular difference in BMI. Sex and age differences were also evaluated by stratified analyses.

Results: From 2014 to 2020, age-adjusted mean BMI increased by 0.3 kg/m (95% : 0.1, 0.5 kg/m) in men, and 0.5 kg/m (95% : 0.2, 0.7 kg/m) in women. Age-standardized prevalence of underweight decreased from 3.0 to 2.3% in men, and from 3.0 to 2.1% in women. Age-standardized prevalence of overweight increased in both men (from 40.1 to 41.7%) and women (from 37.8 to 39.8%), and so as obesity (men: from 4.1 to 6.1%; women: from 5.8 to 8.7%).

Conclusion: Our results confirmed that BMI gradually increased from 2014 to 2020. The age-adjusted mean BMI increased by 0.3 kg/m in older men, and 0.5 kg/m in older women. The age- and sex-standardized prevalence of overweight and obesity significantly increased, especially in 70-79-year age group, while the prevalence of underweight decreased. The combination of a balanced-diet and physical exercise is needed to maintain optimal BMI range for the aged population.
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http://dx.doi.org/10.3389/fnut.2022.923539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253615PMC
June 2022

Biomimetic [email protected] nanozyme platform with Fenton catalytic activity as efficient antibacterial agent.

J Mater Chem B 2022 Jul 27;10(29):5582-5593. Epub 2022 Jul 27.

Key Laboratory of Marine Chemistry Theory and Technology (Ocean University of China), Ministry of Education, Qingdao, 266100, P. R. China.

The multidrug resistance of bacteria caused by the abuse of traditional antibiotics poses a great threat to public health, so it is urgent to develop effective antibacterial agents to deal with this dilemma. Biomimetics and nanotechnology are expected to provide new strategies for solving this problem. This study takes inspiration from the adhesive protein properties of mussels to design and synthesise biomimetic polydopamine nanospheres ([email protected] NPs), which have strong adhesion and catalytic Fenton reactive enzyme activity. The antibacterial activity of [email protected] NPs against () and () is significantly better than that of traditional antibiotics daptomycin (Dap) and vancomycin (Van). PDA NPs with an intrinsic hierarchical structure have the ability to adhere to bacterial surfaces and improve the loading rate of metal ions Fe/Co. In addition, due to the dual effects of strong adhesion and Co, [email protected] NPs can destroy the bacterial membrane structure and release endogenous hydrogen peroxide, which increases the generation of reactive oxygen species by synergistic catalysis of bimetal ions Fe/Co to further kill bacteria thoroughly. The cytotoxicity test results show that [email protected] NPs have good cytocompatibility. The impressive antibacterial properties and good biocompatibility of [email protected] NPs make them a potential antibacterial drug.
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http://dx.doi.org/10.1039/d2tb00588cDOI Listing
July 2022

Spike-mediated ACE2 down-regulation was involved in the pathogenesis of SARS-CoV-2 infection.

J Infect 2022 Jul 3. Epub 2022 Jul 3.

Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital the Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong 518112, China; Guangdong Key laboratory for anti-infection Drug Quality Evaluation, Shenzhen, Guangdong 518112, China; Shenzhen Research Center for Communicable Disease Diagnosis, Treatment of Chinese Academy of Medical Science, Shenzhen, Guangdong 518112, China. Electronic address:

The ongoing global pandemic of Coronavirus disease 2019 (COVID-19) poses a serious threat to human health, with patients reportedly suffering from thrombus, vascular injury and coagulation in addition to acute and diffuse lung injury and respiratory diseases. Angiotensin converting enzyme 2 (ACE2) as the receptor for SARS-CoV-2 entry, is also an important regulator of renin-angiotensin system (RAS) homeostasis, which plays an unsettled role in the pathogenesis of COVID-19. Here, we demonstrated that SARS-CoV-2 Spike protein activated intracellular signals to degrade ACE2 mRNA. The decrease of ACE2 and higher level of angiotensin (Ang) II were verified in COVID-19 patients. High dose of Ang II induced pulmonary artery endothelial cell death in vitro, which was also observed in the lung of COVID-19 patients. Our finding indicates that the downregulation of ACE2 potentially links COVID-19 to the imbalance of RAS.
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http://dx.doi.org/10.1016/j.jinf.2022.06.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250808PMC
July 2022

Clinical features and outcomes of patients with wheat-dependent exercise-induced anaphylaxis: a retrospective study.

Allergy Asthma Clin Immunol 2022 Jul 5;18(1):61. Epub 2022 Jul 5.

Department of Allergy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuaifuyuan Street, Wangfujing, Beijing, 100730, China.

Background: Wheat-dependent exercise-induced anaphylaxis (WDEIA) is a serious and potentially life-threatening form of wheat allergy. Further episodes can only be prevented by avoiding wheat ingestion or avoiding exercise after wheat intake. Anaphylaxis may recur in some patients post-diagnosis. This study aimed to analyze the clinical features and management/outcomes of WDEIA in China.

Methods: We retrospectively analyzed the clinical characteristics, and laboratory testing of 197 patients with WDEIA. After diagnosis, the patients were followed up as outpatients to evaluate dietary/exercise choice and clinical outcomes.

Results: Among the 197 WDEIA patients (median age, 37 years), 53.8% were male and 28.4% had other allergic disorders. The median duration of anaphylaxis before diagnosis was 16 months. Significant delays in diagnosis (> 1 years) were recorded in 52.7% of the patients, which has not decreased by years (P = 0.064). Exercise (83.8%), alcohol (12.2%), and nonsteroidal anti-inflammatory drugs (7.1%) were the most common cofactors. The most common clinical features were urticaria (100%), loss of consciousness (82.7%), dyspnea (50.8%), and hypotension (47.2%). Of the 197 eligible patients, 155 responded (78.7%), and 124 (80.0%) of which had no anaphylaxis post-diagnosis. A wheat-free diet prevented future anaphylaxis in 91.7% of the patients, followed by the avoidance of wheat combined with exercise (87%) and reduced wheat intake combined with exercise avoidance (80.5%).

Conclusion: The diagnosis of WDEIA is frequently delayed. Therefore, when patients present with unexplained anaphylaxis, the possibility of WDEIA should be considered. A wheat-free diet or avoiding wheat combined with exercise or reduced wheat combined with exercise avoidance helps to significantly reduce the onset of future anaphylaxis. However, approximately one-fifth of patients continue to experience anaphylaxis post-diagnosis. Thus, these patients must always carry epinephrine autoinjectors.
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http://dx.doi.org/10.1186/s13223-022-00702-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254488PMC
July 2022

Engineering consortia by polymeric microbial swarmbots.

Nat Commun 2022 Jul 5;13(1):3879. Epub 2022 Jul 5.

CAS Key Laboratory of Quantitative Engineering Biology, Center for Materials Synthetic Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Synthetic microbial consortia represent a new frontier for synthetic biology given that they can solve more complex problems than monocultures. However, most attempts to co-cultivate these artificial communities fail because of the winner-takes-all in nutrients competition. In soil, multiple species can coexist with a spatial organization. Inspired by nature, here we show that an engineered spatial segregation method can assemble stable consortia with both flexibility and precision. We create microbial swarmbot consortia (MSBC) by encapsulating subpopulations with polymeric microcapsules. The crosslinked structure of microcapsules fences microbes, but allows the transport of small molecules and proteins. MSBC method enables the assembly of various synthetic communities and the precise control over the subpopulations. These capabilities can readily modulate the division of labor and communication. Our work integrates the synthetic biology and material science to offer insights into consortia assembly and serve as foundation to diverse applications from biomanufacturing to engineered photosynthesis.
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http://dx.doi.org/10.1038/s41467-022-31467-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256712PMC
July 2022

[Advances in the biomedical application research of halophilic microorganisms].

Sheng Wu Gong Cheng Xue Bao 2022 Jun;38(6):2153-2168

Research Center of Basic Medical Sciences, Medical College, Qinghai University, Xining 810016, Qinghai, China.

In recent years, antibiotic resistance has become increasingly serious, and the number of cancer patients keeps increasing. There is an urgent need to develop new drugs with antibacterial and antitumor effects. Halophilic microorganisms are a special group of microorganisms living in extreme environment. They have the characteristics of metabolic diversity, low nutritional requirements and adaptability to harsh conditions, thus can serve as promising candidates for new drug discovery. To date, researchers have isolated a variety of metabolites and enzymes with antibacterial and/or antitumor activities from halophilic microorganisms. This review summarized the functions and potential biomedical applications of halophilic microorganisms and their related products, such as antibacterial, anti-inflammatory, antitumor, antioxidant, biomedical materials and drug carriers. In particular, novel antibacterial and antitumor substances recently discovered in halophilic microorganisms, as well as the biomedical applications of ectoine, a unique metabolite found in halophilic microorganisms, were introduced. Finally, future development and utilization of halophilic microorganisms in biomedical and industrial fields were prospected.
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http://dx.doi.org/10.13345/j.cjb.210928DOI Listing
June 2022

MiR-1306-5p promotes cell proliferation and inhibits cell apoptosis in acute myeloid leukemia by downregulating PHF6 expression.

Leuk Res 2022 Jun 23;120:106906. Epub 2022 Jun 23.

Department of Medical Basic, Jiangsu College of Nursing, No. 9, Science and Technology Avenue, Huai'an 223005, Jiangsu, China. Electronic address:

Background: Deregulated expression of miRNAs contributes to the development of numerous malignancies, including acute myeloid leukemia (AML). The present work focused on investigating the role of miR-1306-5p in AML pathogenesis and the possible mechanisms.

Methods: The expression levels of miR-1306-5p and PHF6 were assessed in 30 healthy controls and 48 newly diagnosed AML patients. CCK-8 assay, EdU staining, quantitative real-time PCR, TUNEL assay, western blots, and flow cytometry were used to characterize the changes induced by miR-1306-5p or PHF6 overexpression or inhibition. In addition, Starbase and miRWalk databases were adopted to predict the miR-1306-5p target genes.

Results: Here we reported that upregulation of miR-1306-5p was a frequent event in both primary leukemic cells from AML patients and AML cell lines. Functional assays indicated that downregulation of miR-1306-5p leads to AML cell growth arrest, less proliferation, and elevated rates of apoptosis. Mechanistically, miR-1306-5p targets PHF6, a protein that plays a key role in gene transcription regulation. Our data further showed that PHF6 was downregulated in AML patients and cell lines, and depletion of PHF6 expression using RNA interference further enhanced the proliferation while reducing the apoptosis of those leukemic cells.

Conclusion: Our findings show that miR-1306-5p promotes proliferation and prevents apoptosis in AML by directly modulating PHF6 expression and consequently contributes to AML development and progression. miR-1306-5p may function as an oncogene in AML, providing a promising therapeutic target for AML patients.
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http://dx.doi.org/10.1016/j.leukres.2022.106906DOI Listing
June 2022

Identification of priority areas for water ecosystem services by a techno-economic, social and climate change modeling framework.

Water Res 2022 Aug 16;221:118766. Epub 2022 Jun 16.

College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address:

Water scarcity and quality deterioration often occur in economically developing regions, particularly during crises related to climate change or increasing human activities. The assignment of priority areas is considered a suitable strategy for stakeholders to mitigate water crises and cope with water stress. However, most studies focused on protecting water bodies in priority areas and did not consider the hydrological/hydrochemical/hydroecological interaction between aquatic and terrestrial ecosystems. We divided a watershed into manageable areas to select priority areas for multiple water-related ecosystem services (WES-priority areas), considering the aquatic-terrestrial interactions to predict the effects of climate change and human activities. The proposed novelty framework couples the soil and water assessment tool and maximum entropy models with a systematic conservation planning tool. It uses the gross domestic product as the economic cost to assess dynamic changes and social-environmental driving forces. A case study is conducted in the Xiangjiang River basin, a modified watershed of the main tributary of the Yangtze River, China. Results revealed that most of the WES-priority areas were located in the southern and southeastern regions of the upper reaches in all climatic scenarios. The conservation efficiency of the WES-priority areas decreased from 1.264 to 0.867 in 50 years, indicating that the level of protection declined as climate change accelerated. The precipitation was positively correlated with the WES-priority area selection in all climate scenarios. The temperature was only negatively correlated with the WES-priority areas when it exceeded 20 °C, and this effect became more pronounced as the temperature increased. The topographic factors had the most crucial impacts on the upstream priority areas selection. The water flow regulation service played a leading role in identifying WES-priority areas in the middle reaches because the priority areas' distribution here was closely related to the water yield, and its proportion decreased with the acceleration of global warming. The number of WES-priority areas was relatively low in the lower reaches. It was positively associated with the gross domestic product and the amount of built-up land. The proposed framework for WES-priority areas identification enables a sound trade-off between environmental protection and economic development.
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http://dx.doi.org/10.1016/j.watres.2022.118766DOI Listing
August 2022

PEDOT:PSS/CuCl Composite Hole Transporting Layer for Enhancing the Performance of 2D Ruddlesden-Popper Perovskite Solar Cells.

J Phys Chem Lett 2022 Jul 27;13(26):6101-6109. Epub 2022 Jun 27.

State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

Poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) is a popular hole transport layer (HTL) in 2D Ruddlesden-Popper (RP) perovskite solar cell (PSCs) due to its highly conductive, transparent, and solution-processable characteristics. However, fundamental questions such as its strong acidity or mismatched energy level with the 2D RP photoactive layer often restrict the performance and stability of devices. Herein, copper chloride (CuCl), a common direct band gap semiconductor, is doped into PEDOT:PSS, lowering the acidity and tuning the work function of PEDOT:PSS. Due to the improved wettability and the existing chloride in the PEDOT:PSS/CuCl composite substrate, the coated 2D perovskite films exhibit uniform morphology, vertically oriented crystal growth, and enhanced crystallinity. In comparison with controlled devices, the PEDOT:PSS/CuCl based inverted 2D RP PSCs show a maximum power conversion efficiency of 13.36% and long-term stability. The modified PEDOT:PSS overcomes intrinsic imperfections by doping CuCl, indicating that it has a lot of promise for mass production in electrical devices.
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http://dx.doi.org/10.1021/acs.jpclett.2c01399DOI Listing
July 2022
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