Publications by authors named "Xiang Fu"

81 Publications

Adsorption and Desorption Characteristics of Total Flavonoids from on Macroporous Adsorption Resins.

Molecules 2021 Jul 8;26(14). Epub 2021 Jul 8.

College of Agronomy, Liaocheng University, Liaocheng 252000, China.

We examined the application of six different resins with the aim of selecting a macroporous resin suitable for purifying total flavonoids (ASTFs) from crude extract (EAS) by comparing their adsorption/desorption capacities, which led to the selection of HPD-600. Research on the adsorption mechanism showed that the adsorption process had pseudo-second-order kinetics and fit the Freundlich adsorption model. Moreover, the analysis of thermodynamic parameters indicated that the adsorption process is spontaneous and endothermic. The optimal conditions for purification of ASTFs were determined as sample pH of 3, 60% ethanol concentration, and 3 BV·h flow rate, for both adsorption and desorption, using volumes of 2.5 and 4 BV, respectively. The application of macroporous resin HPD-600 to enrich ASTFs resulted in an increase in the purity of total flavonoids, from 28.79% to 50.57%. Additionally, the antioxidant capacity of ASTFs was higher than that of EAS, but both were lower than that of L-ascorbic acid. The changes in ASTFs compositions were determined using ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), with the results illustrating that the levels of seven major flavonoids of ASTFs were increased compared to that in the crude extract.
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http://dx.doi.org/10.3390/molecules26144162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306655PMC
July 2021

Highly Stretchable Shape Memory Self-Soldering Conductive Tape with Reversible Adhesion Switched by Temperature.

Nanomicro Lett 2021 May 11;13(1):124. Epub 2021 May 11.

State Key Laboratory and Institute of Elemento-Organic Chemistry, Centre of Nanoscale Science and Technology and Key Laboratory of Functional Polymer Materials, College of Chemistry, Nankai University, Weijin Road 94, Tianjin, 300071, People's Republic of China.

Highlights: Shape memory self-soldering tape used as conductive interconnecting material. Perfect shape and conductivity memory performance and anti-fatigue performance. Reversible strong-to-weak adhesion switched by temperature. With practical interest in the future applications of next-generation electronic devices, it is imperative to develop new conductive interconnecting materials appropriate for modern electronic devices to replace traditional rigid solder tin and silver paste of high melting temperature or corrosive solvent requirements. Herein, we design highly stretchable shape memory self-soldering conductive (SMSC) tape with reversible adhesion switched by temperature, which is composed of silver particles encapsulated by shape memory polymer. SMSC tape has perfect shape and conductivity memory property and anti-fatigue ability even under the strain of 90%. It also exhibits an initial conductivity of 2772 S cm and a maximum tensile strain of ~ 100%. The maximum conductivity could be increased to 5446 S cm by decreasing the strain to 17%. Meanwhile, SMSC tape can easily realize a heating induced reversible strong-to-weak adhesion transition for self-soldering circuit. The combination of stable conductivity, excellent shape memory performance, and temperature-switching reversible adhesion enables SMSC tape to serve two functions of electrode and solder simultaneously. This provides a new way for conductive interconnecting materials to meet requirements of modern electronic devices in the future.
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http://dx.doi.org/10.1007/s40820-021-00652-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113436PMC
May 2021

Effect of Sleep-Disordered Breathing During Rapid Eye Movement Sleep and Non-Rapid Eye Movement sleep on Acute Ischemic Stroke.

J Stroke Cerebrovasc Dis 2021 Aug 12;30(8):105913. Epub 2021 Jun 12.

Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Department of Neurology, Taicang Affiliated Hospital of Soochow University, The first People's Hospital of Taicang, Taicang 215400, China; Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou 215123, China. Electronic address:

Objectives: Sleep-disordered breathing adversely impacts stroke outcomes. We investigated whether sleep-disordered breathing during rapid eye movement sleep and non-rapid eye movement sleep differentially influenced stroke outcomes.

Materials And Methods: Acute ischemic stroke patients who finished polysomnography within 14 days of stroke onset from April 2010 to August 2018 were reviewed. Patients were divided into four groups according to apnea-hypopnea index during rapid eye movement sleep and non-rapid eye movement sleep. The modified Rankin Scale was used to evaluate short-term outcome. During January and April 2019, another follow-up was performed for long-term outcomes, including stroke-specific quality-of-life scale, modified Rankin Scale, stroke recurrence and death.

Results: Of 140 patients reviewed, 109 were finally recruited. Although patients with sleep-disordered breathing during non-rapid eye movement sleep only and with sleep-disordered breathing during both rapid eye movement sleep and non-rapid eye movement sleep had higher apnea-hypopnea indices and more disrupted sleep structures, short-term and long-term outcomes did not significantly different between four groups. In Logistic regression analysis, apnea-hypopnea index (p = 0.013, OR 1.023, 95%CI 1.005-1.042) was found independently associated with short-term outcome. Rapid eye movement sleep latency (p = 0.045, OR 0.994, 95%CI 0.987-1.000) was found independently associated with quality of life. Apnea-hypopnea indices during rapid eye movement sleep or non-rapid eye movement sleep were not significantly associated with short-term or long-term outcomes.

Conclusions: Apnea-hypopnea index is an independent risk factor of short-term outcome of acute ischemic stroke while sleep-disordered breathing during rapid eye movement sleep and non-rapid eye movement sleep do not affect stroke outcomes differently.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2021.105913DOI Listing
August 2021

Identification of Potent, Selective, and Orally Bioavailable Small-Molecule GSPT1/2 Degraders from a Focused Library of Cereblon Modulators.

J Med Chem 2021 06 27;64(11):7296-7311. Epub 2021 May 27.

Department of Chemical Biology and Therapeutics, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, United States.

Whereas the PROTAC approach to target protein degradation greatly benefits from rational design, the discovery of small-molecule degraders relies mostly on phenotypic screening and retrospective target identification efforts. Here, we describe the design, synthesis, and screening of a large diverse library of thalidomide analogues against a panel of patient-derived leukemia and medulloblastoma cell lines. These efforts led to the discovery of potent and novel GSPT1/2 degraders displaying selectivity over classical IMiD neosubstrates, such as IKZF1/3, and high oral bioavailability in mice. Taken together, this study offers compound (SJ6986) as a valuable chemical probe for studying the role of GSPT1/2 and , and it supports the utility of a diverse library of CRBN binders in the pursuit of targeting undruggable oncoproteins.
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http://dx.doi.org/10.1021/acs.jmedchem.0c01313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201443PMC
June 2021

A Novel TNM Classification for Colorectal Cancers based on the Metro-ticket Paradigm.

J Cancer 2021 5;12(11):3299-3306. Epub 2021 Apr 5.

Department of Gastrointestinal Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China.

Several revisions of the TNM classifications for colorectal cancer (CRC) have acknowledged that the oncological outcomes of stage IIB/IIC CRC are worse than those of stage IIIA. We aimed to develop a novel TNM (nTNM) classification based on the metro-ticket paradigm. We identified eligible CRC patients from the Surveillance, Epidemiology, and End Results database. The nTNM was developed using distance from the origin on a Cartesian plane incorporating the pN (x-axis) and pT (y-axis) stages, and was compared with the AJCC TNM classification. The areas under the curves (AUCs), calibration curves, and Akaike's information criterion (AIC) were used to evaluate the predictive performances of the two classifications. Clinical benefits were further estimated by decision curve analyses. The validation cohort was applied to validate these findings. A total of 58,192 CRC patients (40,736 training cohort, 17,456 validation cohort) were finally included. In the training cohort, 18,476 patients (45.4%) experienced upstaging and 15,907 patients (39.0%) experienced downstaging in the nTNM classification compared with the TNM classification. Taking the prognosis of stage I as the reference, survival decreased with increasing nTNM stage. The nTNM classification showed better discrimination (AUC, 0.678 vs. 0.667, <0.001), model-fitting (AIC, 236,525 vs. 237,741), and clinical benefits than the TNM classification. Similar results were found in the validation cohort. The nTNM classification for CRC has better predictive performances and superior accuracy for predicting prognosis compared with the TNM classification. The nTNM classification should therefore be considered in future revisions of the TNM classification.
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http://dx.doi.org/10.7150/jca.55097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100802PMC
April 2021

Implementing graph-theoretic quantum algorithms on a silicon photonic quantum walk processor.

Sci Adv 2021 Feb 26;7(9). Epub 2021 Feb 26.

Institute for Quantum Information and State Key Laboratory of High Performance Computing, College of Computer Science and Technology, National University of Defense Technology, 410073 Changsha, China.

Applications of quantum walks can depend on the number, exchange symmetry and indistinguishability of the particles involved, and the underlying graph structures where they move. Here, we show that silicon photonics, by exploiting an entanglement-driven scheme, can realize quantum walks with full control over all these properties in one device. The device we realize implements entangled two-photon quantum walks on any five-vertex graph, with continuously tunable particle exchange symmetry and indistinguishability. We show how this simulates single-particle walks on larger graphs, with size and geometry controlled by tuning the properties of the composite quantum walkers. We apply the device to quantum walk algorithms for searching vertices in graphs and testing for graph isomorphisms. In doing so, we implement up to 100 sampled time steps of quantum walk evolution on each of 292 different graphs. This opens the way to large-scale, programmable quantum walk processors for classically intractable applications.
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http://dx.doi.org/10.1126/sciadv.abb8375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909884PMC
February 2021

Sleep disorders and acute stroke: profile and research opportunities.

Chin Med J (Engl) 2021 Feb 23;134(6):631-633. Epub 2021 Feb 23.

Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China.

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http://dx.doi.org/10.1097/CM9.0000000000001426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990017PMC
February 2021

Quantitative analysis of excipient dominated drug formulations by Raman spectroscopy combined with deep learning.

Anal Methods 2021 01 11;13(1):64-68. Epub 2020 Dec 11.

Kongjiang Hospital of Shanghai, Yangpu District, Shanghai, China.

Owing to the growing interest in the application of Raman spectroscopy for quantitative purposes in solid pharmaceutical preparations, an article on the identification of compositions in excipient dominated drugs based on Raman spectra is presented. We proposed label-free Raman spectroscopy in conjunction with deep learning (DL) and non-negative least squares (NNLS) as a solution to overcome the drug fast screening bottleneck, which is not only a great challenge to drug administration, but also a major scientific challenge linked to falsified and/or substandard medicines. The result showed that Raman spectroscopy remains a cost effective, rapid, and user-friendly method, which if combined with DL and NNLS leads to fast implantation in the identification of lactose dominated drug (LDD) formulations. Meanwhile, Raman spectroscopy with the peak matching method allows a visual interpretation of the spectral signature (presence or absence of active pharmaceutical ingredients (APIs) and low content APIs).
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http://dx.doi.org/10.1039/d0ay01874kDOI Listing
January 2021

Remote-controlled quantum computing by quantum entanglement.

Opt Lett 2020 Nov;45(22):6298-6301

Quantum entanglement enables measurement on one party to affect the other's state. Based on this peculiar feature, we propose a model of remote-controlled quantum computing and design an optical scheme to realize this model for a single qubit. As an experimental demonstration of this scheme, we further implement three Pauli operators, Hardmard gate, phase gate, and /8 gate. The minimal fidelity obtained by quantum process tomography reaches 82%. Besides, as a potential application, our model contributes to secure remote quantum information processing.
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http://dx.doi.org/10.1364/OL.401921DOI Listing
November 2020

A Modified Tumor-Node-Metastasis Classification for Stage III Colorectal Cancers Based on Treating Tumor Deposits as Positive Lymph Nodes.

Front Med (Lausanne) 2020 15;7:571154. Epub 2020 Oct 15.

Department of Gastrointestinal Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.

The tumor-node-metastasis classification of the American Joint Committee on Cancer classified tumor deposits (TDs) in patients with colorectal cancer (CRC) without lymph node (LN) metastasis as N1c, but the classification of TDs in patients with LN metastases remains controversial. This study investigated the probability of regarding TDs as positive LNs (pLNs) in pN stage and estimated its prognostic ability in CRC. We used the Surveillance, Epidemiology, and End Results program to analyze CRC patients who underwent surgical therapy (14,906 training cohort, 6,384 validation cohort). A modified pN stage (mpN) was identified using the number of pLNs plus TDs. Overall survival (OS) was analyzed using the Kaplan-Meier survival curves, and significant prognostic factors were identified by univariate and multivariate analyses. Prognostic ability was estimated using the area under the curve (AUC), calibration curve, and the Akaike's information criterion (AIC). Clinical benefit was measured by the decision curve analyses (DCA). The results were validated using the validation cohort. Both the pN and mpN stages were independent prognostic factors in CRC according to univariate and multivariate analyses. The AUC analysis showed that the mpN stage had better prognostic discrimination for OS than the pN stage (0.612 vs. 0.605, < 0.001). The AIC demonstrated that the mpN stage also showed superior model-fitting compared with the pN stage (49,756 vs. 49,841). The DCA further revealed that the mpN stage had better clinical benefits than the pN stage. The validation cohort showed similar findings. We concluded that counting TDs as pLNs may be superior to the pN stage when assessing the prognosis of CRC patients.
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http://dx.doi.org/10.3389/fmed.2020.571154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593244PMC
October 2020

Hacking single-photon avalanche detectors in quantum key distribution via pulse illumination.

Opt Express 2020 Aug;28(17):25574-25590

Quantum key distribution (QKD) has been proved to be information-theoretically secure in theory. Unfortunately, the imperfect devices in practice compromise its security. Thus, to improve the security property of practical QKD systems, a commonly used method is to patch the loopholes in the existing QKD systems. However, in this work, we show an adversary's capability of exploiting the imperfection of the patch itself to bypass the patch. Specifically, we experimentally demonstrate that, in the detector under test, the patch of photocurrent monitor against the detector blinding attack can be defeated by the pulse illumination attack proposed in this paper. We also analyze the secret key rate under the pulse illumination attack, which theoretically confirmed that Eve can conduct the attack to learn the secret key. This work indicates the importance of inspecting the security loopholes in a detection unit to further understand their impacts on a QKD system. The method of pulse illumination attack can be a general testing item in the security evaluation standard of QKD.
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http://dx.doi.org/10.1364/OE.397962DOI Listing
August 2020

Effect of infrahepatic inferior vena cava partial clamping on central venous pressure and intraoperative blood loss during laparoscopic hepatectomy.

Surg Endosc 2021 Jun 30;35(6):2773-2780. Epub 2020 Jun 30.

Department of Hepatobiliary Surgery, Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, 400013, China.

Background: Infrahepatic inferior vena cava (IVC) clamping is considered to be an effective method to reduce central venous pressure (CVP) and intraoperative bleeding in liver resection. However, its efficacy and safety during laparoscopic hepatectomy (LH) remain unclear. We perform this retrospective study to evaluate its efficacy and safety during LH.

Methods: Consecutive patients scheduled for LH from September 2014 to August 2019 were retrospectively reviewed. The intraoperative parameters and postoperative outcomes were analyzed.

Results: All patients in the infrahepatic IVC clamping group were able to tolerate partial clamping of IVC. The CVP was significantly decreased after infrahepatic IVC clamping without hemodynamic instability (8.7 ± 1.4 cmHO vs. 2.1 ± 1.3 cmHO, P = 0.000). Infrahepatic IVC clamping did not significantly reduce total blood loss (287.3 ± 112.5 mL vs. 301.4 ± 127.6 mL, P = 0.133) and blood loss during parenchymal transection (273.2 ± 107.9 mL vs. 296.5 ± 118.1 mL, P = 0.618) compared with the non-clamping group. In subgroup analysis, total blood loss and blood loss during parenchymal transection were significantly reduced in patients with moderate to severe cirrhosis in the clamping group (363.6 ± 71.2 mL vs. 473.4 ± 95.6 mL, P = 0.021), (358.7 ± 70.9 mL vs. 466.9 ± 94.5 mL, P = 0.016), respectively. The complications and hospital stay were comparable.

Conclusions: In conclusion, these data suggest that infrahepatic IVC clamping may be safe and effective.
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http://dx.doi.org/10.1007/s00464-020-07709-yDOI Listing
June 2021

Photocatalytic properties, mechanical strength and durability of TiO/cement composites prepared by a spraying method for removal of organic pollutants.

Chemosphere 2020 Sep 18;254:126813. Epub 2020 Apr 18.

School of Mechanical Engineering, Jiangxi University of Technology, Nanchang, 330098, China. Electronic address:

TiO/cement composites were prepared by a spraying method to degrade organic pollutants. After coated with waterproof liquid, pure cement pastes/mortars were sprayed with TiO suspensions with different TiO contents and spraying times. Photocatalytic properties, mechanical strength and durability were studied. Maximum photocatalytic activity and uniform TiO distribution were achieved at the optimal conditions of 10 wt% TiO content in suspension and 3 spraying times. The TiO/cement pastes had better degradation performance over Rhodamine B (RhB) and methylene blue (MB) than that over methyl orange (MO). After 20 times of cycling degradation, the photocatalytic efficiencies had no significant reduction. The TiO/cement mortars had good mechanical strengths, meeting the mechanical demands of wastewater treatment tanks. In durability, the TiO/cement mortars had better water penetration resistance, chloride penetration resistance and anti-carbonation than pure cement mortars.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126813DOI Listing
September 2020

Metal ion-responsive nanocarrier derived from phosphonated calix[4]arenes for delivering dauricine specifically to sites of brain injury in a mouse model of intracerebral hemorrhage.

J Nanobiotechnology 2020 Apr 19;18(1):61. Epub 2020 Apr 19.

Clinical Research Center for Neurological Diseases of Guangxi Province, The Affiliated Hospital of Guilin Medical University, Guilin, 541001, China.

Primary intracerebral hemorrhage (ICH) is a leading cause of long-term disability and death worldwide. Drug delivery vehicles to treat ICH are less than satisfactory because of their short circulation lives, lack of specific targeting to the hemorrhagic site, and poor control of drug release. To exploit the fact that metal ions such as Fe are more abundant in peri-hematomal tissue than in healthy tissue because of red blood cell lysis, we developed a metal ion-responsive nanocarrier based on a phosphonated calix[4]arene derivative in order to deliver the neuroprotective agent dauricine (DRC) specifically to sites of primary and secondary brain injury. The potential of the dauricine-loaded nanocarriers for ICH therapy was systematically evaluated in vitro and in mouse models of autologous whole blood double infusion. The nanocarriers significantly reduced brain water content, restored blood-brain barrier integrity and attenuated neurological deficits by inhibiting the activation of glial cells, infiltration by neutrophils as well as production of pro-inflammatory factors (IL-1β, IL-6, TNF-α) and matrix-metalloprotease-9. These results suggest that our dauricine-loaded nanocarriers can improve neurological outcomes in an animal model of ICH by reducing inflammatory injury and inhibiting apoptosis and ferroptosis.
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http://dx.doi.org/10.1186/s12951-020-00616-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168846PMC
April 2020

RASS-Enabled S/P-C and S-N Bond Formation for DEL Synthesis.

Angew Chem Int Ed Engl 2020 05 11;59(19):7377-7383. Epub 2020 Mar 11.

Department of Chemistry, Scripps Research, 10550 N. Torrey Pines Road, La Jolla, CA, 92037, USA.

DNA encoded libraries (DEL) have shown promise as a valuable technology for democratizing the hit discovery process. Although DEL provides relatively inexpensive access to libraries of unprecedented size, their production has been hampered by the idiosyncratic needs of the encoding DNA tag relegating DEL compatible chemistry to dilute aqueous environments. Recently reversible adsorption to solid support (RASS) has been demonstrated as a promising method to expand DEL reactivity using standard organic synthesis protocols. Here we demonstrate a suite of on-DNA chemistries to incorporate medicinally relevant and C-S, C-P and N-S linkages into DELs, which are underrepresented in the canonical methods.
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http://dx.doi.org/10.1002/anie.201915493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7486030PMC
May 2020

Reduced cortical arousability to nocturnal apneic episodes in patients with wake-up ischemic stroke.

Sleep Med 2020 02 19;66:252-258. Epub 2019 Sep 19.

Department of Neurology, Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Department of Sleeping Center, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China; Jiangsu Key Laboratory of Neuropsychiatric Diseases, Institute of Neuroscience, Soochow University, Suzhou 215123, China. Electronic address:

Study Objectives: Sleep breathing disorders (SBD) have been linked to wake-up stroke (WUS). Respiratory arousals have an important role in responding to danger during sleep, yet currently no studies have investigated respiratory arousability in WUS. In this study, we used a clinical tool to predict low respiratory arousal threshold (ArTH), and then compared respiratory arousability in patients with WUS and non-WUS.

Methods: We enrolled 119 patients with acute ischemic stroke and assigned them into WUS (n = 34) and non-WUS (n = 85) groups. All participants underwent polysomnography (PSG) during the acute phase of stroke. The respiratory ArTH predictive tool assigns one point for each of the following: apnea-hypopnea index (AHI) < 30/h, nadir oxygen saturation (SaO) > 82.5%, and fraction of hypopneas > 58.3%. An ArTH score ≥2 represents low respiratory ArTH.

Results: Our results reconfirmed the association between moderate-to-severe sleep apnea syndrome and WUS (OR 2.879, 95% CI 1.17-7.089, p = 0.021). Significantly fewer participants with obstructive sleep apnea (AHI ≥ 5/h) had low respiratory ArTH in the WUS group than in the non-WUS group (34.8% vs. 68.1%, respectively, p = 0.008). High respiratory ArTH was independently associated with WUS (OR 5.556, 95% CI 1.959-15.761, p = 0.001).

Conclusions: The correlation between SBD and WUS suggests that sleep apnea might induce acute physiological changes that trigger the onset of stroke. We show that reduced respiratory arousability is associated with WUS, and hypothesize that reduced cortical capability to generate respiratory arousal may have a role in triggering stroke during sleep.
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http://dx.doi.org/10.1016/j.sleep.2019.09.007DOI Listing
February 2020

1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.

Biochem Pharmacol 2020 02 11;172:113752. Epub 2019 Dec 11.

School of Life Sciences, Anhui University, Hefei 230601, China; Center for Stem Cell and Translational Medicine, Anhui University, Hefei 230601, China. Electronic address:

14-3-3τ plays a critical role in tumorigenesis and metastasis of breast cancer and can be used as new drug target protein. Dicaffeoylquinic acids (DCQAs), natural products, have antioxidant, antimicrobial, and anti-inflammatory activities. In this study, the anticancer effects of DCQAs on breast cancer cells MCF-7, MDA-MB-231 cell lines and mechanism in triple negative breast cancer (TNBC) were investigated. First, we screened for DCQAs that could bind to 14-3-3τ and had a significant inhibitory effect on breast cancer cells. MTT, colony formation, transwell migration, and flow cytometric assays revealed that 1,3-DCQA was the best one of 14-3-3τ binding protein from DCQAs against breast cancer cell proliferation and metastasis but safe for normal cells. Through molecular docking simulation, overexpression and knockdown assays, we confirmed that 14-3-3τ was one of 1,3-DCQA target protein. Eukaryotic transcriptome sequencing and western blot analysis demonstrated that 1,3-DCQA binds to 14-3-3τ to prevent breast cancer proliferation and metastasis via Jak/PI3K/Akt and Raf/ERK pathway, which promote IL6 and CSF3 expression raised by CREB (CREBBP, CREB5) and induced cell apoptosis via Bad/Bax/caspase 9 signaling pathway. Our results provided evidence that 1,3-DCQA can be used as a novel lead compound against breast cancer by inhibition of 14-3-3 protein.
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http://dx.doi.org/10.1016/j.bcp.2019.113752DOI Listing
February 2020

General-Purpose Quantum Circuit Simulator with Projected Entangled-Pair States and the Quantum Supremacy Frontier.

Phys Rev Lett 2019 Nov;123(19):190501

Institute for Quantum Information & State Key Laboratory of High Performance Computing, College of Computer, National University of Defense Technology, Changsha 410073, China.

Recent advances on quantum computing hardware have pushed quantum computing to the verge of quantum supremacy. Here, we bring together many-body quantum physics and quantum computing by using a method for strongly interacting two-dimensional systems, the projected entangled-pair states, to realize an effective general-purpose simulator of quantum algorithms. The classical computing complexity of this simulator is directly related to the entanglement generation of the underlying quantum circuit rather than the number of qubits or gate operations. We apply our method to study random quantum circuits, which allows us to quantify precisely the memory usage and the time requirements of random quantum circuits. We demonstrate our method by computing one amplitude for a 7×7 lattice of qubits with depth (1+40+1) on the Tianhe-2 supercomputer.
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http://dx.doi.org/10.1103/PhysRevLett.123.190501DOI Listing
November 2019

Polyethylene glycol-based ultrasonic-assisted enzymatic extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from leaves.

Food Sci Nutr 2019 Nov 13;7(11):3452-3462. Epub 2019 Sep 13.

Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains Huanggang Normal University Huangzhou China.

In this paper, polyethylene glycol (PEG)-based ultrasonic-assisted enzymatic extraction (UAEE) was employed in polysaccharides extraction from leaves (LJLP). The optimal extraction conditions (extraction time of 33 min, PEG concentration of 30%, and ultrasonic power of 191 W) were obtained by Box-Behnken design (BBD). Under this condition, the LJLP yield was 14.76%. Furthermore, LJLP was identified as a typical heteropolysaccharide considering the main constitutive monosaccharides include galactose (32.3%), glucose (20.9%), and ribose (15.2%). Moreover, LJLP exhibited high total reducing power and considerable scavenging activities on superoxide radicals, in a concentration-dependent manner in vitro. In addition, antioxidant analysis in vivo revealed that for the LJLP-treated mice, the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities, and total antioxidant capacity (TAOC) were significantly increased, while the level of malondialdehyde (MDA) was decreased in both serum and liver.
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http://dx.doi.org/10.1002/fsn3.1186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848850PMC
November 2019

[Development and application of the plant phenomics analysis platform].

Yi Chuan 2019 Nov;41(11):1060-1066

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100101, China.

With the completion of the whole genome sequencing of major important crops, researchers have an increasing demand for high-throughput, accurate and nondestructive phenotyping technologies. The Plant Phenomics Analysis Platform (PPAP) was established in 2017 at the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences. The platform has the most up-to-date comprehensive phenotyping analysis facility in China with a full spectrum of imaging systems consisting of eight units including visible light, infrared, near-infrared, root near-infrared, fluorescence, chlorophyll fluorescence, high spectral and lidar imaging. The platform has also specifically established phenotyping technologies for complex traits, such as root phenotype collection and analysis, spike and spikelet feature collection and analysis and responses under stress conditions. PPAP is dedicated to providing all-possible services for domestic and international academic communities and industrial partners engaged in plant sciences.
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http://dx.doi.org/10.16288/j.yczz.19-277DOI Listing
November 2019

Total saponins extracted from Abrus cantoniensis Hance suppress hepatitis B virus replication in vitro and in rAAV8-1.3HBV transfected mice.

J Ethnopharmacol 2020 Mar 31;249:112366. Epub 2019 Oct 31.

Research Center for Clinical Pharmacology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. Electronic address:

Ethnopharmacological Relevance: Hepatitis B, an infectious disease caused by hepatitis B virus (HBV), is still a serious problem affecting global public health. Abrus cantoniensis Hance (AC), a traditional Chinese medicinal herb, has been used as a folk medicine for treating hepatitis in China from ancient times. However, its active ingredients are still unclear.

Aim Of Study: Our previous study indicated that saponins extracted from AC (ACS) were the active anti-HBV ingredients in AC. This study aimed to further investigate the anti-HBV effect of ACS in vitro and in vivo.

Materials And Methods: HepG2.2.15 cells which consecutively produce HBV DNA and HBV antigens were used for in vitro test, and C57BL/6 mice infected by a recombinant adeno-associated virus 8 vector carrying 1.3 copies of HBV genome (rAAV8-HBV1.3) were used for in vivo test. The histopathological changes and the immune indices were evaluated in mice model. Genechip was conducted to identify genes and pathways regulated by ACS in HepG2.2.15 cells.

Results: In this study, we confirmed that ACS treatment prominently inhibited production of HBV DNA, Hepatitis Be Antigen (HBeAg), and Hepatitis B surface antigen (HBsAg) in HepG2.2.15 cells. ACS treatment also decreased serum HBsAg, HBeAg, and HBV DNA level in rAAV8-1.3HBV transfected mice, which is in accordance with the in vitro results. Moreover, HBV infection-induced liver inflammation was significantly relieved by ACS, which could be observed in H&E staining and immunohistochemistry of HBcAg. ACS treatment elevated IFN-γ level in mice serum and increased CD4 T cell percentage in splenocytes. KEGG pathway analysis showed that phenylalanine metabolism pathway and tyrosine metabolism pathway were greatly regulated by ACS treatment.

Conclusion: ACS exerted potent inhibitory effects on HBV replication both in vivo and in vitro, which may provide basis for its potential clinical usage.
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http://dx.doi.org/10.1016/j.jep.2019.112366DOI Listing
March 2020

Synthesis and Kinetics of the -(2-Methyl-6-ethyl phenyl)-1-methoxypropyl-2-imine Schiff Base Catalyzed by NKC-9 Cation Exchange Resin.

ACS Omega 2019 Sep 5;4(12):14750-14758. Epub 2019 Sep 5.

School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.

The kinetics of condensation reaction of methoxyacetone with 2-methyl-6-ethyl aniline catalyzed by NKC-9 cation exchange resin was studied for the first time. The reaction temperature of Schiff base synthesis was determined in the range of 367.15 to 401.15 K by the batch experiments, and influences of reactant molar ratio, temperature, catalyst dosage, and particle size on the ultimate conversion were also studied. The dynamic data were used to be relevant with PH, ER(1), ER(2), and Langmuir Hinshelwood Hougen Watson homogeneity models. Model parameters, including reaction equilibrium constants, activation energy, enthalpy change, entropy change, and rate constants, were solved. The accuracy of the model was validated by means of both experimental proofs and standard deviation between the predicted and experimental data. Finally, a series of characterization tests such as Fourier transform infrared spectroscopy, X-ray diffraction, and polarizing microscopy were performed to investigate the structure and properties of NKC-9.
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http://dx.doi.org/10.1021/acsomega.9b01197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756530PMC
September 2019

Asymmetric Multicomponent Reactions for Efficient Construction of Homopropargyl Amine Carboxylic Esters.

Org Lett 2019 07 1;21(14):5737-5741. Epub 2019 Jul 1.

Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, School of Pharmaceutical Sciences , Sun Yat-sen University , Guangzhou 510006 , China.

Developing an efficient and highly enantioselective protocol to access homopropargyl amines is of high interest to the synthetic community and also remains a formidable challenge for organic chemists. Here, we present integrated Rh(OAc)- and BINOL-derived chiral phosphoric acid cooperatively catalyzed three-component reactions of alkynyldiazoacetates, imines with various nucleophiles including alcohols, indoles, and N,N-disubstituted anilines, affording the corresponding homopropargyl amines containing two vicinal chiral centers in satisfactory yields with high to excellent diastereo- and enantioselectivities.
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http://dx.doi.org/10.1021/acs.orglett.9b02139DOI Listing
July 2019

Targeting PARP and autophagy evoked synergistic lethality in hepatocellular carcinoma.

Carcinogenesis 2020 05;41(3):345-357

Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, P. R. China.

Hepatocellular carcinoma (HCC), one of the most lethal malignancies worldwide, has limited efficient therapeutic options. Here, we first demonstrated that simultaneously targeting poly (ADP-ribose) polymerase (PARP) and autophagy could evoke striking synergistic lethality in HCC cells. Specifically, we found that the PARP inhibitor Niraparib induced cytotoxicity accompanied by significant autophagy formation and autophagic flux in HCC cells. Further experiments showed that Niraparib induced suppression of the Akt/mTOR pathway and activation of the Erk1/2 cascade, two typical signaling pathways related to autophagy. In addition, the accumulation of reactive oxygen species was triggered, which was involved in Niraparib-induced autophagy. Blocking autophagy by chloroquine (CQ) in combination with Niraparib further enhanced cytotoxicity, induced apoptosis and inhibited colony formation in HCC cells. Synergistic inhibition was also observed in Huh7 xenografts in vivo. Mechanistically, we showed that autophagy inhibition abrogated Niraparib-induced cell-cycle arrest and checkpoint activation. Cotreatment with CQ and Niraparib promoted the formation of γ-H2AX foci while inhibiting the recruitment of the homologous recombination repair protein RAD51 to double-strand break sites. Thus, the present study developed a novel promising strategy for the management of HCC in the clinic and highlighted a potential approach to expand the application of PARP inhibitors.
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http://dx.doi.org/10.1093/carcin/bgz104DOI Listing
May 2020

Stretchable and sensitive sensor based on carbon nanotubes/polymer composite with serpentine shapes via molding technique.

J Biomater Sci Polym Ed 2019 09 13;30(13):1227-1241. Epub 2019 Jun 13.

a School of Engineering, Computer and Mathematical Sciences, Institute of Biomedical Technologies, Auckland University of Technology , Auckland , New Zealand.

With the rapid development of wearable devices in recent years, stretchable strain sensors based on electrically conductive composites have attracted a great deal of attention owing to their good stretchability and piezoresistivity. However, due to the intrinsic restriction of these types of composites, the conventional stretchable strain sensors cannot do well in all aspect of sensing performance. A stretchable strain sensor based on carbon nanotubes/poly(dimethylsiloxane) composite with the serpentine shape was devised and fabricated. The sensor was readily manufactured through a molding technique. Not only can this sensor distinguish tension strain from transverse or longitudinal direction, but also exhibits good linearity of response to tensile strain. In terms of sensitivity, hysteresis and response time, the stretchable strain sensor showed significant performance. The sensing performance of this proposed stretchable sensor has been demonstrated to be good in this work and it also shows a good prospect for utilization in multifunctional wearable devices.
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http://dx.doi.org/10.1080/09205063.2019.1627649DOI Listing
September 2019

Asymmetric Bargaining Model for Water Resource Allocation over Transboundary Rivers.

Int J Environ Res Public Health 2019 05 16;16(10). Epub 2019 May 16.

State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan 430072, China.

Sustainable transboundary water governance is often challenged by conflicts between agents, which necessitates the design of cooperative and self-enforcing alternatives to facilitate equitable water distribution. The Nash bargaining approach, which originated from game theory, could offer a good mathematical framework to simulate strategic interactions among involved agents by considering individual rational benefits. Given that river-sharing problems often involve multiple self-interested agents, the asymmetric Nash bargaining solution (ANBS) could be used to describe agents' powers, as determined by disparate social, economic, and political as well as military status, and ensure win-win strategies based on individual rationality. This paper proposed an asymmetric bargaining model by combining multi-criteria decision making, bankruptcy theory, and the ANBS for water distribution in the transboundary river context. The Euphrates River Basin (ERB) with three littoral states was used as a case study. Turkey has the highest bargaining power in ERB negotiation since it dominates in terms of economic strength, political influence, and military capacity, whereas in the two downstream countries these aspects are limited due to their internal political fragmentation and weaker military status. The water satisfaction percentages of Turkey, Syria, and Iraq under the best alternative are 96.30%, 84.23%, and 40.88%, respectively. The findings highlight the necessity for synthetically considering the agent's disagreement utility and asymmetrical power when negotiating over water allocation.
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http://dx.doi.org/10.3390/ijerph16101733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571634PMC
May 2019

Simulation of UV power absorbed by follicular stem cells during sun exposure and possible implications for melanoma development.

J Opt Soc Am A Opt Image Sci Vis 2019 Apr;36(4):628-635

The incidence of melanoma in children is extremely rare. However, there is convincing evidence supporting a higher risk of developing melanoma in individuals who have experienced excessive sun exposure during their childhood. A possible explanation for this phenomenon is that prior to puberty, the melanocyte stem cells in the bulge region of hair follicles are much shallower in the dermis, resulting in their increased vulnerability to sun exposure. To validate this hypothesis, a Monte Carlo simulation of light transport is applied to quantify the dose of solar UV power absorbed in the stem-cell layers at different depths in both child and adult skins. The simulated results suggest that the stem cells in vellus hair follicles would absorb over 250 times higher UV photons than those in the terminal hair follicles. Due to the thinner epidermis in child skin, the stem cells in vellus hair follicles absorb about 1.9 and 3.2 times greater ultraviolet A and ultraviolet B, respectively, than those in adult skin. These findings provide a possible explanation to why children are particularly vulnerable to sun exposure.
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http://dx.doi.org/10.1364/JOSAA.36.000628DOI Listing
April 2019

Role of the HIF-1 signaling pathway in chronic obstructive pulmonary disease.

Exp Ther Med 2018 Dec 21;16(6):4553-4561. Epub 2018 Sep 21.

Department of Respiratory Medicine, The No. 5 Hospital of Xiamen, Xiamen, Fujian 361100, P.R. China.

Chronic obstructive pulmonary disease (COPD) is the most common cause of chronic morbidity and mortality. However, the molecular mechanisms underlying COPD remain largely unknown. The purpose of the present study was to investigate the expression patterns of hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF), and VEGF receptor 2 (R2) in regard to the HIF-1 signaling pathway in COPD. The expressions of HIF-1α, VEGF and VEGFR2 were examined and quantified in the human lung tissues of 102 subjects with a defined smoking status, with or without COPD. The expressions of HIF-1α, VEGF and VEGFR2 were observed to be increased in the lung tissues collected from smoking COPD subjects when compared with those tissues from smoking subjects without COPD and non-smoking subjects without COPD. The expression of HIF-1α was shown to be positively associated with the expression of VEGF and VEGFR2. In addition, increased expression of HIF-1α, VEGF and VEGFR2 reflected the disease severity of COPD. The key findings obtained from the present study indicated that high expression of HIF-1α, VEGF and VEGFR2 may be associated with decreased lung function and reduced quality of life, contributing to disease progression in COPD.
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http://dx.doi.org/10.3892/etm.2018.6785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257248PMC
December 2018

Diversity and Niche of Archaea in Bioremediation.

Archaea 2018 3;2018:3194108. Epub 2018 Sep 3.

School of Civil and Environmental Engineering, College of Engineering, Architecture, and Technology, Oklahoma State University, Stillwater, OK 74078, USA.

Bioremediation is the use of microorganisms for the degradation or removal of contaminants. Most bioremediation research has focused on processes performed by the domain ; however, are known to play important roles in many situations. In extreme conditions, such as halophilic or acidophilic environments, are well suited for bioremediation. In other conditions, collaboratively work alongside during biodegradation. In this review, the various roles that have in bioremediation is covered, including halophilic hydrocarbon degradation, acidophilic hydrocarbon degradation, hydrocarbon degradation in nonextreme environments such as soils and oceans, metal remediation, acid mine drainage, and dehalogenation. Research needs are addressed in these areas. Beyond bioremediation, these processes are important for wastewater treatment (particularly industrial wastewater treatment) and help in the understanding of the natural microbial ecology of several genera.
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http://dx.doi.org/10.1155/2018/3194108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6140281PMC
August 2019

The reasonable drainage option after laparoscopic common bile duct exploration for the treatment of choledocholithiasis.

Clin Res Hepatol Gastroenterol 2018 12 23;42(6):564-569. Epub 2018 Aug 23.

Department of hepatobiliary surgery, Chongqing general hospital, 400013 Chongqing, People's Republic of China. Electronic address:

Objective: To obtain a reasonable drainage after laparoscopic common bile duct exploration (LCBDE) for the treatment of choledocholithiasis.

Methods: Data of 350 consecutive patients who underwent LCBDE in our hospital from January 2014 to December 2016 were retrospectively reviewed. All the patients were divided into three groups according to different drainage types after LCBDE, including T-tube group with 116 cases, primary closure (PC) group with 114 cases and stent insertion group with 120 cases. Operative parameters and outcomes were compared.

Results: The operative time was no significant difference between the T-tube group (106.71 ± 5.19 min), PC group (105.46 ± 5.77 min) and stent insertion group (106.88 ± 5.91 min) (F = 2.175, P = 0.115). The postoperative hospital stay was significantly shorter in the stent insertion group (5.62 ± 0.70 d) than in the T-tube group (7.79 ± 0.85 d) and PC group (7.60 ± 0.80 d) (F = 279.649, P = 0.000). The hospitalization cost was significantly less in the stent insertion group (19,432.78 ± 661.74 yuan) than in the T-tube group (22,059.90 ± 697.98 yuan) and PC group (21,927.20 ± 772.02 yuan) (F = 512.492, P = 0.000). The incidence of postoperative biliary-specific complications was 2.59% (3/116 cases) in the T-tube group, 2.63% (3/114 cases) in the PC group, and 0% (0/120 cases) in the stent insertion group, but this difference was not statistically significant (χ = 3.177, P = 0.204). The return to normal levels of postoperative liver function tests (LFTs) was significantly faster in the stent insertion group and T-tube group than in the PC group (P < 0.05). The number of 314 patients were followed up for a median time of 20 months (range from 1-48 months), and no biliary stricture, cholangitis or stone recurrence occurred in these patients during that time.

Conclusions: Stent insertion shows better results when compared with T-tube drainage and primary duct closure in terms of postoperative hospital stay and hospitalization cost. It is the prior option for the choledochotomy closure after LCBDE in suitable patients.
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http://dx.doi.org/10.1016/j.clinre.2018.07.005DOI Listing
December 2018
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