Publications by authors named "Xiang Chen"

1,608 Publications

  • Page 1 of 1

Clinical Significance of Plasma D-Dimer in COVID-19 Mortality.

Front Med (Lausanne) 2021 25;8:638097. Epub 2021 May 25.

Hunan Key Laboratory of Skin Cancer and Psoriasis, Department of Dermatology, Hunan Engineering Research Center of Skin Health and Disease, Xiangya Hospital, Central South University, Changsha, China.

It is not clear whether D-dimer can be an independent predictor of coronavirus disease 2019 (COVID-19) mortality, and the cut-off of D-dimer for clinical use remains to be determined. Therefore, a comprehensive analysis is still necessary to illuminate the clinical significance of plasma D-dimer in COVID-19 mortality. We searched PubMed, Embase, Cochrane Library, and Scopus databases until November 2020. STATA software was used for all the statistical analyses. The identifier of systematic review registration was PROSPERO CRD42020220927. A total of 66 studies involving 40,614 COVID-19 patients were included in our meta-analysis. Pooled data showed that patients in high D-dimer group had poor prognosis than those in low D-dimer group [OR = 4.52, 95% CI = (3.61, 5.67), < 0.001; HR = 2.81, 95% CI = (1.85, 4.27), < 0.001]. Sensitivity analysis, pooled data based on different effect models and the Duval and Tweedie trim-and-fill method did not change the conclusions. Subgroup analyses stratified by different countries, cutoffs, sample size, study design, and analysis of OR/HR still keep consistent conclusions. D-dimer was identified as an independent predictor for COVID-19 mortality. A series of values including 0.5 μg/ml, 1 μg/ml, and 2 μg/ml could be determined as cutoff of D-dimer for clinic use. Measurement and monitoring of D-dimer might assist clinicians to take immediate medical actions and predict the prognosis of COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2021.638097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185282PMC
May 2021

Advanced Devices for Tumor Diagnosis and Therapy.

Small 2021 Jun 10:e2100003. Epub 2021 Jun 10.

Institute of Optoelectronics & Nanomaterials, MIIT Key Laboratory of Advanced Display Materials and Devices, School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.

At present, tumor diagnosis is performed using common procedures, which are slow, costly, and still presenting difficulties in diagnosing tumors at their early stage. Tumor therapeutic methods also mainly rely on large-scale equipment or non-intelligent treatment approaches. Thus, an early and accurate tumor diagnosis and personalized treatment may represent the best treatment option for a successful result, and the efforts in finding them are still in progress and mainly focusing on non-destructive, integrated, and multiple technologies. These objectives can be achieved with the development of advanced devices and smart technology that represent the topic of the current investigations. Therefore, this review summarizes the progress in tumor diagnosis and therapy and briefly explains the advantages and disadvantages of the described microdevices, finally proposing advanced micro smart devices as the future development trend for tumor diagnosis and therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202100003DOI Listing
June 2021

Accurate genomic variant detection in single cells with primary template-directed amplification.

Proc Natl Acad Sci U S A 2021 Jun;118(24)

Department of Pediatrics, Stanford University, Stanford, CA 94304;

Improvements in whole genome amplification (WGA) would enable new types of basic and applied biomedical research, including studies of intratissue genetic diversity that require more accurate single-cell genotyping. Here, we present primary template-directed amplification (PTA), an isothermal WGA method that reproducibly captures >95% of the genomes of single cells in a more uniform and accurate manner than existing approaches, resulting in significantly improved variant calling sensitivity and precision. To illustrate the types of studies that are enabled by PTA, we developed direct measurement of environmental mutagenicity (DMEM), a tool for mapping genome-wide interactions of mutagens with single living human cells at base-pair resolution. In addition, we utilized PTA for genome-wide off-target indel and structural variant detection in cells that had undergone CRISPR-mediated genome editing, establishing the feasibility for performing single-cell evaluations of biopsies from edited tissues. The improved precision and accuracy of variant detection with PTA overcomes the current limitations of accurate WGA, which is the major obstacle to studying genetic diversity and evolution at cellular resolution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2024176118DOI Listing
June 2021

Establishment of cultured primary acral melanoma cells and animal models for Chinese patients.

Pigment Cell Melanoma Res 2021 Jun 7. Epub 2021 Jun 7.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Acral melanoma is the major subtype of melanoma in Chinese patients. However, a majority of current studies focused on non-acral melanoma. Most immortalized melanoma cell lines and primary cells were not from acral melanoma. Besides, there are rarely reports about methods for establishing primary acral melanoma cell cultures and related animal models. Here, we present four new human primary acral melanoma cell lines. To determine the mutational profile of the established primary melanoma cells for future targeted use, we performed exome sequencing. We next examined cell proliferation of the primary acral melanoma cells by colony-formation assays and CCK8 assay. We also evaluated the proliferative and metastatic potential of XYAM-4 in vivo. We report a detailed protocol for establishing cultured primary acral melanoma cells for Chinese patients and related animal models. We also summarize the features in our acral melanoma cell lines and the exist acral melanoma cell lines. This will provide an effective research tool for research on drug responses and individualized treatment for Chinese patients and comparative studies of melanomas between western and Chinese populations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/pcmr.12996DOI Listing
June 2021

Electrodeposition of (hydro)oxides for an oxygen evolution electrode.

Chem Sci 2020 Apr 20;11(39):10614-10625. Epub 2020 Apr 20.

Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), Renewable Energy Conversion and Storage Center, College of Chemistry, Nankai University Tianjin 300071 China

Electrochemical water splitting is a promising technology for hydrogen production and sustainable energy conversion, but the electrolyzers that are currently available do not have anodic electrodes that are robust enough and highly active for the oxygen evolution reaction (OER). Electrodeposition provides a feasible route for preparing freestanding OER electrodes with high active site utilization, fast mass transport and a simple fabrication process, which is highly attractive from both academic and commercial points of view. This minireview focuses on the recent electrodeposition strategies for metal (hydro)oxide design and water oxidation applications. First, the intrinsic advantages of electrodeposition in comparison with traditional technologies are introduced. Then, the unique properties and underlying principles of electrodeposited metal (hydro)oxides in the OER are unveiled. In parallel, illustrative examples of the latest advances in materials structural design, controllable synthesis, and mechanism understanding through the electrochemical synthesis of (hydro)oxides are presented. Finally, the latest representative OER mechanism and electrodeposition routes for OER catalysts are briefly overviewed. Such observations provide new insights into freestanding (hydro)oxides electrodes prepared electrodeposition, which show significant practical application potential in water splitting devices. We hope that this review will provide inspiration for researchers and stimulate the development of water splitting technology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0sc01532fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162381PMC
April 2020

CXCL2/10/12/14 are prognostic biomarkers and correlated with immune infiltration in hepatocellular carcinoma.

Biosci Rep 2021 Jun;41(6)

Department of Science and Education, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, China.

Background: C-x-C motif chemokine ligands (CXCLs) are critical regulators of cancer immunity and angiogenesis, which affect disease progression and treatment responses. The character of each CXCL in the prognosis and immune infiltration of hepatocellular carcinoma (HCC) patients is unclear yet.

Methods: Differentially expressed CXCLs between HCC and normal control were screened by Oncomine and GEPIA2. Genetic alternations of CXCLs in HCC were analyzed by cBioPortal. Clinicopathological relevance of CXCLs in HCC patients was analyzed using UALCAN. The prognostic value of CXCLs was evaluated using univariate and multivariate analyses. Correlations of CXCLs' expression with immune infiltration, chemokines and their receptors were assessed integrating TIMER, TISIDB, and GEPIA2. The co-expressed genes of CXCLs were discovered, and functional enrichment analysis was performed for them.

Results: CXCL9/10 was significantly higher expressed while CXCL2/12/14 was lower expressed in HCC than normal tissues, but they didn't show significant clinicopathological relevance in HCC patients. High-expression of CXCL2/10/12/14 indicated favorable outcomes of HCC patients. The expression of CXCL9/10/12/14 was significantly positively correlated with not only the infiltration and biomarkers' expression of various tumor-infiltrating immune cells but also the abundance of chemokines and their receptors. The co-expressed genes of the five CXCLs were extracellular components and regulated immune or inflammatory responses and signaling pathways of chemokine, Toll-like receptor and tumor necrosis factor might be involved.

Conclusion: The present study proposed CXCL2/10/12/14 might predict outcomes of HCC patients and were extensively related with the immune microenvironment in HCC. It would be a prospective therapeutic strategy for HCC to enhance effective immunity surveillance through intervening in these CXCLs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1042/BSR20204312DOI Listing
June 2021

A Bayesian Network Meta-analysis of Comparison of Cancer Therapeutic Vaccines for Melanoma.

J Eur Acad Dermatol Venereol 2021 Jun 2. Epub 2021 Jun 2.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.

Several approaches to active immunotherapy for melanoma, including peptide-based vaccines (PVs), autologous tumor cell vaccines (TCVs), allogeneic TCVs, and autologous dendritic cell vaccines (DCVs), have been investigated in clinical trials. However, comprehensive evidence comparing these interventions remains unavailable. The objective of this study was to expand previous work to compare and rank the immunotherapeutic strategies for melanoma in terms of overall survival and toxic effects with a Bayesian network meta-analysis. Methodologically, we performed a network meta-analysis of head-to-head randomized controlled trials comparing and ranking cancer vaccine approaches for patients with melanoma. PubMed, MEDLINE, Embase, the Cochrane Central Register of Controlled Trials, the WHO International Clinical Trials Registry Platform, and ClinicalTrials.gov were searched up to July 31, 2020. We estimated summary hazard ratios for death and risk ratios for toxicity. The effects of the underlying prognostic variable on survival benefits were examined by meta-regression. We performed subgroup analysis for the outcomes based on metastatic categories. Overall, we identified 4776 citations, of which 15 head-to-head randomized controlled trials (3162 participants) were included in the analysis. In terms of efficacy, allogeneic tumor cell vaccines plus immunotherapy adjuvants, peptide-based vaccines plus immunotherapy adjuvants, and standard therapy were more effective than peptide vaccines. The proportion of women was inversely associated with mortality risk. For safety, all treatments were inferior to allogeneic tumor cell vaccines except for allogeneic tumor cell vaccines plus chemotherapy. Peptide vaccines plus immunotherapy adjuvants led to an increased risk of adverse events compared to allogeneic tumor cell vaccines plus immunotherapy adjuvants. These results suggest that allogeneic TCV and autologous DCV are better than standard therapy. PV plus immune modulators is the most effective strategy among all comparable strategies but is associated with increased toxicity. Any combination regimens for cancer therapeutic vaccines need to be balanced between risk and benefit profiles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jdv.17437DOI Listing
June 2021

Laser-assisted photodynamic therapy vs. conventional photodynamic therapy in non-melanoma skin cancers: Systematic review and meta-analysis of randomized controlled trials.

Photodermatol Photoimmunol Photomed 2021 Jun 2. Epub 2021 Jun 2.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/phpp.12700DOI Listing
June 2021

cGAS restricts colon cancer development by protecting intestinal barrier integrity.

Proc Natl Acad Sci U S A 2021 Jun;118(23)

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390;

The DNA-sensing enzyme cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) regulates inflammation and immune defense against pathogens and malignant cells. Although cGAS has been shown to exert antitumor effects in several mouse models harboring transplanted tumor cell lines, its role in tumors arising from endogenous tissues remains unknown. Here, we show that deletion of cGAS in mice exacerbated chemical-induced colitis and colitis-associated colon cancer (CAC). Interestingly, mice lacking cGAS were more susceptible to CAC than those lacking stimulator of interferon genes (STING) or type I interferon receptor under the same conditions. cGAS but not STING is highly expressed in intestinal stem cells. cGAS deficiency led to intestinal stem cell loss and compromised intestinal barrier integrity upon dextran sodium sulfate-induced acute injury. Loss of cGAS exacerbated inflammation, led to activation of STAT3, and accelerated proliferation of intestinal epithelial cells during CAC development. Mice lacking cGAS also accumulated myeloid-derived suppressive cells within the tumor, displayed enhanced Th17 differentiation, but reduced interleukin (IL)-10 production. These results indicate that cGAS plays an important role in controlling CAC development by defending the integrity of the intestinal mucosa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2105747118DOI Listing
June 2021

Versatile nanocellulose-based nanohybrids: A promising-new class for active packaging applications.

Int J Biol Macromol 2021 May 28;182:1915-1930. Epub 2021 May 28.

Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.

The food packaging industry is rapidly growing as a consequence of the development of nanotechnology and changing consumers' preferences for food quality and safety. In today's globalization of markets, active packaging has achieved many advantages with the capability to absorb or release substances for prolonging the food shelf life over the traditional one. Therefore, it is critical to developing multifunctional active packaging materials from biodegradable polymers with active agents to decrease environmental challenges. This review article addresses the recent advances in nanocelluloses (NCs)- baseds nanohybrids with new function features in packaging, focusing on the various synthesis methods of NCs-based nanohybrids, and their reinforcing effects as active agents on food packaging properties. The applications of NCs-based nanohybrids as antioxidants, antimicrobial agents, and UV blocker absorbers for prolonging food shelf-life are also reviewed. Overall, these advantages make the CNs-based nanohybrids with versatile properties promising in food and packaging industries, which will impact more readership with concern for future research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.05.169DOI Listing
May 2021

Differential Metabolites in Chinese Autistic Children: A Multi-Center Study Based on Urinary H-NMR Metabolomics Analysis.

Front Psychiatry 2021 11;12:624767. Epub 2021 May 11.

Department of Neurology, Children's Hospital of Fudan University, Shanghai, China.

Autism spectrum disorder (ASD) is a group of early-onset neurodevelopmental disorders. However, there is no valuable biomarker for the early diagnosis of ASD. Our large-scale and multi-center study aims to identify metabolic variations between ASD and healthy children and to investigate differential metabolites and associated pathogenic mechanisms. One hundred and seventeen autistic children and 119 healthy children were recruited from research centers of 7 cities. Urine samples were assayed by H-NMR metabolomics analysis to detect metabolic variations. Multivariate statistical analysis, including principal component analysis (PCA), and orthogonal projection to latent structure discriminant analysis (OPLS-DA), as well as univariate analysis were used to assess differential metabolites between the ASD and control groups. The differential metabolites were further analyzed by receiver operating characteristics (ROC) curve analysis and metabolic pathways analysis. Compared with the control group, the ASD group showed higher levels of glycine, guanidinoacetic acid, creatine, hydroxyphenylacetylglycine, phenylacetylglycine, and formate and lower levels of 3-aminoisobutanoic acid, alanine, taurine, creatinine, hypoxanthine, and N-methylnicotinamide. ROC curve showed relatively significant diagnostic values for hypoxanthine [area under the curve (AUC) = 0.657, 95% CI 0.588 to 0.726], creatinine (AUC = 0.639, 95% CI 0.569 to 0.709), creatine (AUC = 0.623, 95% CI 0.552 to 0.694), N-methylnicotinamide (AUC = 0.595, 95% CI 0.523 to 0.668), and guanidinoacetic acid (AUC = 0.574, 95% CI 0.501 to 0.647) in the ASD group. Combining the metabolites creatine, creatinine and hypoxanthine, the AUC of the ROC curve reached 0.720 (95% CI 0.659 to 0.777). Significantly altered metabolite pathways associated with differential metabolites were glycine, serine and threonine metabolism, arginine and proline metabolism, and taurine and hypotaurine metabolism. Urinary amino acid metabolites were significantly altered in children with ASD. Amino acid metabolic pathways might play important roles in the pathogenic mechanisms of ASD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyt.2021.624767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144639PMC
May 2021

High levels of truncated RHAMM cooperate with dysfunctional p53 to accelerate the progression of pancreatic cancer.

Cancer Lett 2021 Aug 24;514:79-89. Epub 2021 May 24.

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, 10065, USA. Electronic address:

Pancreatic cancer has the lowest survival rate out of all types of cancer. Pancreatic cancer patients are often diagnosed at advanced stages, hence an urgent need for a better therapeutic development of this devastating disease. Receptor for hyaluronan-mediated motility (RHAMM), not expressed in adult normal pancreas, has been suggested as a prognostic factor and a potential therapeutic target for pancreatic ductal adenocarcinoma (PDAC) and pancreatic neuroendocrine tumor (PNET). In this study, we initially sought to determine whether genetic deletion of RHAMM would slow down pancreatic cancer progression using Rhamm mice. However, we found that Rhamm mice expressed a truncated HMMR protein at higher abundance levels than wild-type RHAMM. While HMMR did not enable malignant progression of pancreatic intraepithelial neoplasia in p48-Cre; LSL-KRAS mice, it accelerated the formation of invasive PDAC and shortened the survival of p48-Cre; LSL-KRAS mice with heterozygous p53 knockout. Kras PDAC mice with homozygous p53 knockout mice died around 10 weeks, and the effect of HMMR was not apparent in these short lifespan mice. In addition, HMMR shortened the survival of PNET-bearing RIP-Tag mice, which had inactivated p53. In our analysis of TCGA dataset, pancreatic cancer patients with mutant TP53 or loss of one copy of TP53 had higher RHAMM expression, which, combined, predicted worse outcomes. Taken together, by collaborating with dysfunctional p53, high levels of HMMR , which lacks the centrosome targeting domain and degrons for interaction with the Anaphase-Promoting Complex (APC), accelerated pancreatic cancer progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2021.05.011DOI Listing
August 2021

Application of 3D printing technology in endophytic reninoma: A case report and literature review.

Clin Nephrol 2021 May 25. Epub 2021 May 25.

Background: A reninoma is a rare benign renal tumor that leads to secondary aldosteronism, secondary hypertension, and hypokalemia due to excessive renin secretion.

Case Presentation: We report a case of a patient presenting with typical endophytic reninoma and describe her diagnosis and treatment. Following 3D reconstruction from computed tomography images combined with pre-operative planning via 3D printing technology, partial nephrectomy was performed; the scope of resection was accurately evaluated to achieve precise tumor excision. After surgery, the patient's plasma renin and aldosterone levels decreased rapidly; her serum potassium level normalized 1 week later, and her blood pressure also gradually returned to normal.

Discussion And Conclusion: Our objective is to highlight the diagnostic evaluation for reninoma. To the best of our knowledge, this is the first case report to demonstrate the advantages of 3D printing technology for endophytic reninoma resection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5414/CN110469DOI Listing
May 2021

Association between transferrin saturation and celiac disease: a two-sample Mendelian randomization study.

Pediatr Allergy Immunol 2021 May 24. Epub 2021 May 24.

Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha, China, 410078.

Celiac disease (CD) refers to a chronic bowel disorder related to the disturbance of immune systems and intolerance to gluten. Globally, CD affects about 1.4% of the total population with various gastrointestinal and extraintestinal symptoms . While genetical background plays significant roles in the occurrence of CD, emerging evidence from observational studies demonstrate strong associations of CD with other chronic diseases, indicating shared etiology or risk factors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/pai.13565DOI Listing
May 2021

The effect of eIF3a on anthracycline-based chemotherapy resistance by regulating DSB DNA repair.

Biochem Pharmacol 2021 May 20;190:114616. Epub 2021 May 20.

Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, PR China; Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, PR China; National Clinical Research Center for Geriatric Disorder, Xiangya Hospital, Central South University, PR China; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, 110 Xiangya Road, Changsha 410078, PR China; Hunan Key Laboratory of Precise Diagnosis and Treatment of Gastrointestinal Tumor, Changsha 410078, PR China. Electronic address:

Background: Anthracycline are inhibitors of topoisomerase II leading to DNA double strand breaks, and it is widely used for treatment of breast cancer. eIF3a is the largest subunit of eukaryotic translation initiation factor 3 (eIF3) and highly expressed in breast cancer. In this study, we investigated the role of eIF3a in DSB DNA repair and the response of breast cancer patients to anthracycline-based chemotherapy.

Methods: MTT assay was used to detect anthracycline sensitivity in cell lines. Real-time reverse transcriptase PCR, western blotting and immunofluorescence were performed to assess changes in gene expression levels. Cometassay and end-joining activity assay were conducted to explore the effect of eIF3a in NHEJ repair. Luciferase reporter assay was performed to detect LIG4 5'UTR activity. Immunohistochemistry was used to detect eIF3a, LIG4 and DNA-PKcs expression levels in breast cancer tissues.

Results: The results showed that eIF3a increased cellular response to anthracyclines by regulating DSB repair activity via influencing the expression of LIG4 and DNA-PKcs at translational level. Breast cancer patients with high level of eIF3a or low level of LIG4 or low level of DNA-PKcs had better anthracycline-based chemotherapy prognosis compared. Moreover, Combined expressions of eIF3a, LIG4 and DNA-PKcs could be better to predict PFS in breast cancer patients with anthracycline-based chemotherapy.

Conclusion: Our findings suggest that eIF3a effects anthracycline-based chemotherapy response by regulating DSB DNA repair.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bcp.2021.114616DOI Listing
May 2021

Prevalence of acne in Chinese college students and its associations with social determinants and quality of life: a population-based cross-sectional study.

Chin Med J (Engl) 2021 Jan 11;134(10):1239-1241. Epub 2021 Jan 11.

Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/CM9.0000000000001292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143762PMC
January 2021

Evaluation of Beta-Cyfluthrin Resistance of Cigarette Beetle (Coleoptera: Anobiidae) from Cigarette Manufacturing Factories of China and Underlying Metabolic Mechanisms Responsible for Resistance.

J Econ Entomol 2021 May 18. Epub 2021 May 18.

Department of Applied Chemistry, University of Science and Technology of China, Hefei, 230026, China.

Beta-cyfluthrin, as a synthetic pyrethroid, has been widely used in cigarette manufacturing factories in China to control Lasioderma serricorne (F.) (Coleoptera: Anobiidae). In this study, spray toxicity bioassays and filter paper residual contact toxicity bioassays were conducted to investigate the beta-cyfluthrin sensitivity level of five field strains of L. serricorne collected from cigarette manufacturing factories in China. Bioassay results indicated that five field strains had developed different levels of resistance to beta-cyfluthrin with RR50 of 3.51-10.20 at 2 hr after application and 4.05-49.50 at 24 hr after application in spray toxicity bioassays, and RR50 of 4.74-14.47 at 2 hr exposure in filter paper residual contact bioassays. In addition, we examined CarE, GST, and CYP450 enzyme activity and content of L. serricorne adults and larvae. Enzyme-linked immunosorbent assay results suggested that there was no significant difference in GST, CYP450, and CarE content of L. serricorne adults between field strains and reference sensitive strain. Biochemical assay results indicated that CYP450 activity of L. serricorne adults and larvae of five field strains was significantly higher than that of reference sensitive strain, with increased CYP450 activity of 1.08-1.82-fold in adults and 1.08-2.12-fold in larvae. The results implied that elevated CYP450 activity may contribute to metabolic resistance of L. serricorne to pyrethroid. Our study indicated that there was no clear evidence that the enhanced CarE and GST activity was associated with pyrethroid resistance of L. serricorne.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jee/toab093DOI Listing
May 2021

Exploring the possible targeting strategies of liposomes against methicillin-resistant Staphylococcus aureus (MRSA).

Eur J Pharm Biopharm 2021 Aug 8;165:84-105. Epub 2021 May 8.

Centre for Drug Delivery Technology, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. Electronic address:

Multi antibiotic-resistant bacterial infections are on the rise due to the overuse of antibiotics. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the pathogens listed under the category of serious threats where vancomycin remains the mainstay treatment despite the availability of various antibacterial agents. Recently, decreased susceptibility to vancomycin from clinical isolates of MRSA has been reported and has drawn worldwide attention as it is often difficult to overcome and leads to increased medical costs, mortality, and longer hospital stays. Development of antibiotic delivery systems is often necessary to improve bioavailability and biodistribution, in order to reduce antibiotic resistance and increase the lifespan of antibiotics. Liposome entrapment has been used as a method to allow higher drug dosing apart from reducing toxicity associated with drugs. The surface of the liposomes can also be designed and enhanced with drug-release properties, active targeting, and stealth effects to prevent recognition by the mononuclear phagocyte system, thus enhancing its circulation time. The present review aimed to highlight the possible targeting strategies of liposomes against MRSA bacteremia systemically while investigating the magnitude of this effect on the minimum inhibitory concentration level.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejpb.2021.04.021DOI Listing
August 2021

[Cloning and expression of the growth hormone gene and its effect on the proliferation of skeletal muscle cells of Guizhou cattle].

Sheng Wu Gong Cheng Xue Bao 2021 Apr;37(4):1249-1259

Key Laboratory of Animal Genetics and Breeding and Reproduction of Plateau and Mountain Animals of Guizhou University, Guizhou Key Laboratory of Animal Genetics and Breeding and Reproduction, Guiyang 550025, Guizhou, China.

The aim of this study was to investigate the expression of growth hormone (GH) gene on skeletal muscle cell proliferation of Guizhou cattle. The coding sequence of cattle GH gene was amplified by reverse transcription PCR, cloned into the pUCM-T vector and then used to construct the GH gene overexpression vector pEGFP-N3-GH. The expression of the GH gene in skeletal muscle-related tissues (psoas major and longissimus dorsi) of Guizhou cattle was determined by real-time fluorescent quantitative PCR (RT-qPCR). This was followed by culturing and identification of the bovine primary skeletal muscle cells. Subsequently, we introduced the GH gene overexpression vector into the cells to investigate its effect on the proliferation of bovine skeletal muscle cells and the expression of insulin like growth factor 1 and 2 genes related to skeletal muscle growth and development. RT-qPCR results showed that the expression level of GH gene was higher in the psoas major than in the longissimus dorsi of Guizhou cattle, and the expression level in the psoas major of Guanling cattle and Weining cattle was significantly higher than in the longissimus dorsi (P<0.05). The transfection and proliferation results showed that pEGFP-N3-GH significantly increased the expression of GH, IGF-1, and IGF-2 genes in skeletal muscle cells compared to pEGFP-N3 (PP<0.05), and that overexpression of the GH gene also significantly increased the proliferation rate of skeletal muscle cells at the four periods examined (PP<0.01). Our results suggest that GH gene can promote the proliferation of skeletal muscle cells of Guizhou cattle and exerts a positive regulatory effect. This lays the foundation for further exploring the mechanism by which the GH gene affects the growth and development of Guizhou cattle.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13345/j.cjb.200460DOI Listing
April 2021

HIF1 may promote glycolysis in psoriasis vulgaris via upregulation of CD147 and GLUT1.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Apr;46(4):333-344

Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha 410008.

Objectives: To analyze the expressions and distributions of hypoxia-inducible factor-1α (HIF-1α), CD147, and glucose transporter 1 (GLUT1) in epidermis from psoriasis vulgaris and normal people, and to explore the associations among these proteins and their roles in hypoxic HaCaT cell line.

Methods: The expression levels of HIF-1α, CD147, and GLUT1 were determined by immunohistochemistry staining in skin biopsies from 48 psoriasis vularis patients and 33 healthy subjects. Cobalt chloride (CoCl) was added into the culture media of HaCaT cells to mimic hypoxia while RNA interference and transfection technologies were used to explore the association among these proteins by quantitative real-time polymerase chain reaction and Western blotting. Glycolytic capacity was detected by ATP and lactate measurements.

Results: HIF-1α, CD147, and GLUT1 were highly expressed and the glycolytic capacity was increased in lesions of psoriasis vulgaris; HIF-1α upregulated the expression of CD147 and GLUT1, increased the lactate production and decreased the ATP level in CoCl-treated HaCaT cells, while CD147 and GLUT1 directly or indirectly bound to each other.

Conclusions: Glycolytic capacity increases in the injured keratinocytes of psoriasis vulgaris, suggesting that HIF-1α, CD147, and GLUT1 are associated with glycolysis, which can be considered as the promising targets for psoriasis therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200010DOI Listing
April 2021

Molecular characterization of cathelicidin in tiger frog (Hoplobatrachus rugulosus): Antimicrobial activity and immunomodulatory activity.

Comp Biochem Physiol C Toxicol Pharmacol 2021 May 7;247:109072. Epub 2021 May 7.

College of Ecology, Lishui University, Lishui 323000, China. Electronic address:

Cathelicidins are an important antimicrobial peptide family and are expressed in many different vertebrates. They play an important role in the innate immune system of the host. However, amphibian cathelicidins are poorly understood. In this study, the cDNA of the cathelicidin gene was obtained from the skin transcriptome of tiger frog (Hoplobatrachus rugulosus). The predicted amino acid sequence of tiger frog cathelicidin (HR-CATH) comprises a signal peptide, a cathelin domain, and a mature peptide. The HR-CATH amino acid sequence alignment with other frog cathelicidins showed that the functional mature peptide is highly variable in amphibians, whereas the cathelin domain is conserved. A phylogenetic tree analysis showed that HR-CATH is most closely related to cathelicidin-NV from Nanorana ventripunctata. HR-CATH was chemically synthesized and its in vitro activity was determined. It had high antibacterial activity against Vibrio parahaemolyticus, Staphylococcus aureus, and the pathogenic bacterium Aeromonas hydrophila. HR-CATH damaged the cell membrane integrity of A. hydrophila according to a lactate dehydrogenase release assay and was able to hydrolyze the genomic DNA from A. hydrophila in a dose-dependent manner. Furthermore, in RAW264.7 cells (mouse leukemic monocyte/macrophage cell line), HR-CATH induced chemotaxis and enhanced respiratory burst. Our study shows that amphibian cathelicidin has antimicrobial activity and an immunomodulatory effect on immune cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpc.2021.109072DOI Listing
May 2021

A Novel User Emotional Interaction Design Model Using Long and Short-Term Memory Networks and Deep Learning.

Front Psychol 2021 20;12:674853. Epub 2021 Apr 20.

School of Design, Jiangnan University, Wuxi, China.

Emotional design is an important development trend of interaction design. Emotional design in products plays a key role in enhancing user experience and inducing user emotional resonance. In recent years, based on the user's emotional experience, the design concept of strengthening product emotional design has become a new direction for most designers to improve their design thinking. In the emotional interaction design, the machine needs to capture the user's key information in real time, recognize the user's emotional state, and use a variety of clues to finally determine the appropriate user model. Based on this background, this research uses a deep learning mechanism for more accurate and effective emotion recognition, thereby optimizing the design of the interactive system and improving the user experience. First of all, this research discusses how to use user characteristics such as speech, facial expression, video, heartbeat, etc., to make machines more accurately recognize human emotions. Through the analysis of various characteristics, the speech is selected as the experimental material. Second, a speech-based emotion recognition method is proposed. The mel-Frequency cepstral coefficient (MFCC) of the speech signal is used as the input of the improved long and short-term memory network (ILSTM). To ensure the integrity of the information and the accuracy of the output at the next moment, ILSTM makes peephole connections in the forget gate and input gate of LSTM, and adds the unit state as input data to the threshold layer. The emotional features obtained by ILSTM are input into the attention layer, and the self-attention mechanism is used to calculate the weight of each frame of speech signal. The speech features with higher weights are used to distinguish different emotions and complete the emotion recognition of the speech signal. Experiments on the EMO-DB and CASIA datasets verify the effectiveness of the model for emotion recognition. Finally, the feasibility of emotional interaction system design is discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2021.674853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093774PMC
April 2021

Can Aqueous Zinc-Air Batteries Work at Sub-Zero Temperatures?

Angew Chem Int Ed Engl 2021 May 3. Epub 2021 May 3.

Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.

Efficient energy storage at low temperatures starves for competent battery techniques. Herein, inherent advantages of zinc-air batteries on low-temperature electrochemical energy storage are discovered. The electrode reactions are resistive against low temperatures to render feasible working zinc-air batteries under sub-zero temperatures. The relatively reduced ionic conductivity of electrolyte is identified as the main limiting factor, which can be addressed by employing a CsOH-based electrolyte through regulating the solvation structures. Accordingly, 500 cycles with a stable voltage gap of 0.8 V at 5.0 mA cm is achieved at -10 °C. This work reveals the promising potential of zinc-air batteries for low-temperature electrochemical energy storage and inspires advanced battery systems under extreme working conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202104171DOI Listing
May 2021

Electrolyte Structure of Lithium Polysulfides with Anti-Reductive Solvent Shells for Practical Lithium-Sulfur Batteries.

Angew Chem Int Ed Engl 2021 Apr 29. Epub 2021 Apr 29.

Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, P. R. China.

The lithium-sulfur (Li-S) battery is regarded as a promising secondary battery. However, constant parasitic reactions between the Li anode and soluble polysulfide (PS) intermediates significantly deteriorate the working Li anode. The rational design to inhibit the parasitic reactions is plagued by the inability to understand and regulate the electrolyte structure of PSs. Herein, the electrolyte structure of PSs with anti-reductive solvent shells was unveiled by molecular dynamics simulations and nuclear magnetic resonance. The reduction resistance of the solvent shell is proven to be a key reason for the decreased reactivity of PSs towards Li. With isopropyl ether (DIPE) as a cosolvent, DIPE molecules tend to distribute in the outer solvent shell due to poor solvating power. Furthermore, DIPE is more stable than conventional ether solvents against Li metal. The reactivity of PSs is suppressed by encapsulating PSs into anti-reductive solvent shells. Consequently, the cycling performance of working Li-S batteries was significantly improved and a pouch cell of 300 Wh kg was demonstrated. The fundamental understanding in this work provides an unprecedented ground to understand the electrolyte structure of PSs and the rational electrolyte design in Li-S batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202103470DOI Listing
April 2021

Insulin receptor is implicated in triple-negative breast cancer by decreasing cell mobility.

J Biomed Res 2020 Aug;35(3):189-196

Department of Medical Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, Jiangsu 211166, China.

Triple-negative breast cancer (TNBC) has a poor prognosis and typically earlier onset of metastasis in comparison with other breast cancer subtypes. It has been reported that insulin receptor (INSR) is downregulated in TNBC, however, its clinical significance and functions in TNBC remain to be elucidated. In this study, we found that INSR expression was significantly downregulated in TNBC, and overexpression of INSR suppressed cell migration and invasion in TNBC. In addition, the survival rate of breast cancer patients with low INSR expression was lower than that of patients with high INSR expression. INSR expression was significantly correlated with lymph node metastasis, clinical tumor stages, ER status, PR status, and the proliferation index Ki-67 expression. In summary, our study suggests that INSR may serve as a biomarker for breast cancer prognosis and it may be a potential target for TNBC treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7555/JBR.34.20200082DOI Listing
August 2020

Effects of Anisotropy and In-Plane Grain Boundary in Cu/Pd Multilayered Films with Cube-on-Cube and Twinned Interface.

Nanoscale Res Lett 2021 Apr 28;16(1):69. Epub 2021 Apr 28.

College of Mechanical Engineering, Chongqing University, Chongqing, 400044, China.

In crystalline materials, grain boundary and anisotropy of crystal structure affect their mechanical properties. The effects of interfacial structure on the mechanical properties may be diverse when the multilayer film is loaded along different directions. In this work, we performed a series of molecular dynamics simulations of the tension of in-plane single and polycrystalline Cu/Pd multilayered films with cube-on-cube (COC) and twinned interfaces to explore the effects of the interfacial structure, loading direction and in-plane grain boundaries on their mechanical properties. The interfacial misfit dislocation lines become bent after relaxation, and the high temperature of 300 K was found as a necessary condition. When stretched along 〈110〉 direction, the strengthening effect of the COC interface is more noticeable; however, when stretched along 〈112〉 direction, the twin interface's strengthening effect is more visible, showing the anisotropic effect of interfacial structure on mechanical properties. However, in the in-plane honeycomb polycrystalline sample, the twin interface showed a pronounced strengthening effect, and no jogged dislocations were observed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s11671-021-03528-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081810PMC
April 2021

ER stress modulates apoptosis in A431 cell subjected to EtNBSe-PDT via the PERK pathway.

Photodiagnosis Photodyn Ther 2021 Apr 24;34:102305. Epub 2021 Apr 24.

Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan Province, China. Electronic address:

Photodynamic therapy (PDT) is a promising modality against various cancers including squamous cell carcinoma (SCC) with which the induction of apoptosis is an effective mechanism. Here, we initially describe the preclinical activity of 5-ethylamino-9-diethylaminobenzo [a] phenoselenazinium(EtNBSe)-mediated PDT treatment in SCC. Results of our studies suggest that EtNBSe-PDT provokes a cellular state of endoplasmic reticulum (ER) stress triggering the PERK/ eIF2α signaling pathway and induces the appearance of apoptosis in A431 cells at the meantime. With ER stress inhibitor 4-PBA or eIF2α inhibitor ISRIB, suppressing the EtNBSe-PDT induced ER stress substantially promotes apoptosis of A431 cells. Furthermore, we demonstrate that ATF4, whose expression is ER-stress-inducible and elevated in response to the PERK/eIF2α signaling pathway activation, contributes to cytoprotection against EtNBSe-PDT induced apoptosis. In a mouse model bearing A431 cells, EtNBSe shows intense phototoxicity and when associated with decreased ER stress, EtNBSe-PDT ameliorates tumor growth. Taken together, our study reveals an antagonistic activity of ER stress against EtNBSe-PDT treatment via inhibiting apoptosis in A431 cells. With further development, these results provide a proof-of-concept that downregulation of ER stress response has a therapeutic potential to improve EtNBSe-PDT sensitivity in SCC patients via the promotion of induced apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pdpdt.2021.102305DOI Listing
April 2021

ADMETlab 2.0: an integrated online platform for accurate and comprehensive predictions of ADMET properties.

Nucleic Acids Res 2021 Apr 24. Epub 2021 Apr 24.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, Hunan, China.

Because undesirable pharmacokinetics and toxicity of candidate compounds are the main reasons for the failure of drug development, it has been widely recognized that absorption, distribution, metabolism, excretion and toxicity (ADMET) should be evaluated as early as possible. In silico ADMET evaluation models have been developed as an additional tool to assist medicinal chemists in the design and optimization of leads. Here, we announced the release of ADMETlab 2.0, a completely redesigned version of the widely used AMDETlab web server for the predictions of pharmacokinetics and toxicity properties of chemicals, of which the supported ADMET-related endpoints are approximately twice the number of the endpoints in the previous version, including 17 physicochemical properties, 13 medicinal chemistry properties, 23 ADME properties, 27 toxicity endpoints and 8 toxicophore rules (751 substructures). A multi-task graph attention framework was employed to develop the robust and accurate models in ADMETlab 2.0. The batch computation module was provided in response to numerous requests from users, and the representation of the results was further optimized. The ADMETlab 2.0 server is freely available, without registration, at https://admetmesh.scbdd.com/.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/nar/gkab255DOI Listing
April 2021

Effects of nFeO capping on phosphorus release from sediments in a eutrophic lake.

Environ Sci Pollut Res Int 2021 Apr 22. Epub 2021 Apr 22.

College of Hydrology and Water Resources, Hohai University, Nanjing, 210098, China.

This study applied the techniques of high-resolution dialysis (HR-Peeper) and diffusive gradients in thin films (DGT) to explore the effects and the behind mechanism for inhibition phosphorus (P) releasing from sediments by nFeO capping. The highest decreasing rates of SRP and labile P (i.e., 49% and 47%, respectively) and the decreased flux of SRP showed that nFeO capping can successfully control sediment internal P release. Adsorption by Fe(III) hydroxides with the oxidation of Fe(II) was one of the reasons for the decrease of P concentrations in nFeO capping sediments. This was supported by the increase of Eh and significant negative correlation between Eh with Fe(II) (soluble and labile Fe(II)) and P (SRP and labile P) and significant positive correlation between Fe(II) and P in sediments by nFeO capping. An outer-sphere complex between positively charged nFeO surface groups and P formation was the other reason to decrease the concentrations of P in the nFeO capping sediments. This was supported by the decrease of pH value in sediments by the capping of nFeO. This study shows that nFeO, when used as capping agent, can effectively control the sediment internal P release, which is expected to be used as a potential material for repairing lake eutrophication.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-14010-5DOI Listing
April 2021

Photoelectrochemical detection of human epidermal growth factor receptor 2 (HER2) based on CoO-ascorbic acid oxidase as multiple signal amplifier.

Mikrochim Acta 2021 Apr 20;188(5):166. Epub 2021 Apr 20.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, 410008, China.

A sensitive photoelectrochemical (PEC) sensor based on hexagonal carbon nitride tubes (HCNT) as photoactive material was prepared for the detection of human epidermal growth factor receptor 2 (HER2). Magnetic FeO nanospheres (MNs) modified with anti-HER2 antibodies were employed for highly efficient capture of HER2 from serum sample, and CoO nanoparticles (CoO NPs) modified with ascorbic acid oxidase (AAO) as well as HER2 aptamer were used for signal amplification. When the aptamer-CoO-AAO probe was captured onto the electrode surface through the specific binding of the aptamer with HER2, the photocurrent intensity decreased. This was because CoO NPs competed with HCNT for consumption of the excitation energy. As a consequence AAO catalyzed the oxidation of the electron donor (AA), and the aptamer-CoO-AAO probe increased the steric hindrance at the electrode surface, leading to significant photocurrent intensity decrease, thus realizing multiple signal amplification. Based on this signal amplification strategy, at 0 V (vs Ag/AgCl), the PEC sensor shows a wide linear response ranging from 1 pg mL to 1 ng mL with a low detection limit of 0.026 pg mL for HER2. Importantly, the prepared PEC sensor was applied for detection of HER2 in human serum samples with recoveries between 98.8 and 101%. Sensitive photoelectrochemical sensor based on CoO nanoparticles modified with ascorbic acid oxidase for signal amplification is reported.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-021-04829-7DOI Listing
April 2021