Publications by authors named "Xia Zhang"

1,454 Publications

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Circ_0001658 regulates gefitinib resistance of non-small cell lung cancer through miR-409-3p/TWIST1 axis.

Anticancer Drugs 2021 Oct 22. Epub 2021 Oct 22.

School of Pharmacy, Ningxia Medical University Department of Stomatology, Yinchuan Stomatological Hospital, Yinchuan, Ningxia, China.

Background: To investigate the function and mechanism of circular RNA circ_0001658 on gefitinib resistance of non-small cell lung cancer (NSCLC) cells.

Methods: Circ_0001658, microRNA (miRNA, miR)-409-3p and twist family bHLH transcription factor 1 (TWIST1) expression levels in NSCLC tissues and cell lines were probed by quantitative real-time PCR and Western blot assays. Cell counting kit-8 assay was adopted to examine the inhibitory effect of different concentrations of gefitinib on the viability of NSCLC cells, with the 50% concentration of inhibition (IC50) value calculated. Besides, BrdU assay and flow cytometry assay were used to detect the proliferative and apoptotic rate of NSCLC cells. What's more, the binding relationships between miR-409-3p and circ_0001658, miR-409-3p and TWIST1 mRNA 3' untranslated region (3' UTR) were confirmed by dual-luciferase reporter gene assay and RNA immunoprecipitation assay.

Results: Circ_0001658 expression was raised in NSCLC tissue samples and cell lines, which was significantly associated with TNM stage and the differentiation degree of NSCLC tissues. Knocking down circ_0001658 could restrain the viability of NSCLC cells, promote the apoptosis, and reduce the IC50 of gefitinib, while transfection of miR-409-3p inhibitors could partially reverse these impacts. Additionally, circ_0001658 directly targeted miR-409-3p and negatively modulated its expression. TWIST1 was the target of miR-409-3p, which could be indirectly and positively modulated by circ_0001658. Moreover, circ_0001658 expression was negatively interrelated with miR-409-3p expression, while positively correlated with TWIST1 expression in NSCLC samples.

Conclusion: Circ_0001658 promotes the malignant phenotypes and the resistance to gefitinib of NSCLC cells by regulating the miR-409-3p/TWIST1 axis.
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http://dx.doi.org/10.1097/CAD.0000000000001257DOI Listing
October 2021

TDG is a pig-specific epigenetic regulator with insensitivity to H3K9 and H3K27 demethylation in nuclear transfer embryos.

Stem Cell Reports 2021 Oct 4. Epub 2021 Oct 4.

Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction (Huazhong Agricultural University), Ministry of Education, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China. Electronic address:

Pig cloning by somatic cell nuclear transfer (SCNT) frequently undergoes incomplete epigenetic remodeling during the maternal-to-zygotic transition, which leads to a significant embryonic loss before implantation. Here, we generated the first genome-wide landscapes of histone methylation in pig SCNT embryos. Excessive H3K9me3 and H3K27me3, but not H3K4me3, were observed in the genomic regions with unfaithful embryonic genome activation and donor-cell-specific gene silencing. A combination of H3K9 demethylase KDM4A and GSK126, an inhibitor of H3K27me3 writer, were able to remove these epigenetic barriers and restore the global transcriptome in SCNT embryos. More importantly, thymine DNA glycosylase (TDG) was defined as a pig-specific epigenetic regulator for nuclear reprogramming, which was not reactivated by H3K9me3 and H3K27me3 removal. Both combined treatment and transient TDG overexpression promoted DNA demethylation and enhanced the blastocyst-forming rates of SCNT embryos, thus offering valuable methods to increase the cloning efficiency of genome-edited pigs for agricultural and biomedical purposes.
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http://dx.doi.org/10.1016/j.stemcr.2021.09.012DOI Listing
October 2021

Direct laser writing of vapour-responsive photonic arrays.

J Mater Chem C Mater 2021 Sep 2;9(35):11674-11678. Epub 2021 Sep 2.

School of Chemistry & AMBER, The SFI Research Centre for Advanced Materials and BioEngineering Research, Trinity College Dublin Dublin 2 Ireland

Using direct laser writing, arrays of optically responsive ionogel structures were fabricated. To demonstrate their responsive nature, visible colour changes in the presence of different solvent vapours were investigated. This represents a new departure for photonic structural colouration, in which the fabricating structure shows a programmable, controllable, and dynamic stimuli response.
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http://dx.doi.org/10.1039/d1tc01796aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444305PMC
September 2021

Application Effect of Medical Care Integration Combined with Family Intervention under the Evidence-Based Nursing Mode on Child Patients with Severe Hand-Foot-Mouth Disease and Its Influence on Intestinal Function.

Evid Based Complement Alternat Med 2021 11;2021:9599711. Epub 2021 Oct 11.

Endoscopy Room, The Fourth People's Hospital of Jinan, The Third Affiliated Hospital of Shandong First Medical University, Jinan 250031, Shandong Province, China.

Objective: To explore the application effect of medical care integration combined with family intervention under the evidence-based nursing mode on child patients with severe hand-foot-mouth disease (HFMD) and its influence on intestinal function.

Methods: 120 child patients with severe HFMD admitted to Qilu Children's Hospital of Shandong University from January 2019 to January 2020 were selected as the research object and randomly divided into group A and group B, with 60 cases each. Conventional nursing was performed on patients in group B, and medical care integration combined with family intervention under the evidence-based nursing mode was performed on patients in group A. Patients were assessed after the intervention using the hospital-made treatment adherence scale, PedsQLTM 4.0 (Pediatric Quality of Life Inventory Version 4.0) scale, and the faces pain scale-revised (FPS-R). The levels of gastrointestinal function indicators such as serum endotoxin (ET), diamine oxidase (DAO), and d-lactic acid (D-LA) were measured before and after the intervention, and recovery such as time to clear fever and time to relief of oral pain were recorded in both groups.

Results: Children in group A had significantly higher compliance in diet, behaviour, exercise, and medication than group B ( < 0.05); the time to clear fever, time to relief of oral pain, time to healing of oral ulcers, time to relief of skin herpes, time to hospitalization, and time to eating were shorter in group A than those in group B ( < 0.001); all scores on the PedsQLTM 4.0 scale were higher in group A than those in group B after the intervention ( < 0.001); ET, DAO, and D-LA levels decreased in both groups after the intervention, with group A having lower levels than group B ( < 0.001), in addition, group A had lower eating pain scores after the intervention ( < 0.05).

Conclusion: Medical care integration combined with family intervention under the evidence-based nursing mode can effectively improve the treatment compliance of child patients with severe HFMD, accelerate their recovery progress, ensure a better prognostic quality of life and gastrointestinal tract function, and reduce the eating pain, indicating that such comprehensive nursing intervention mode should be promoted in practice.
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http://dx.doi.org/10.1155/2021/9599711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523275PMC
October 2021

How to Achieve Standardization? Diluted Russell Viper Venom Test for Lupus Anticoagulant Detection in a Chinese Female Population.

Clin Appl Thromb Hemost 2021 Jan-Dec;27:10760296211048897

Department of Laboratory Medicine, West China Second University Hospital, Sichuan University, Chengdu, China.

On an international scale, guidelines and proposals for lupus anticoagulant detection have been published over the last 20 years, but until now, standardization has not been completely realized. The aim of this study was to evaluate the different ways of interpreting the results of lupus anticoagulant detection for standardization. A retrospective review of 15 447 instances of lupus anticoagulant detection by the diluted Russell Viper Venom test for female patients presenting with problems relating to the areas of reproduction, gynecology and obstetrics was performed. Lupus anticoagulant data were compared between different departments, months, reagent lots and cutoffs. Significant differences were found in patient data between different reagent lots, especially between lots of screening reagents (monthly average: highest 37.96 s vs lowest 33.88 s) and in the positive rates of lupus anticoagulant by different detection cutoffs (47.58% by using LA1/LA2 > 1.20 without normalization as a cutoff in Lot 1 vs 1.52% by using LA1 > 44 s as a cutoff in Lot 3). Compared with the cutoff using the value above the 99th percentile of LA1 for the healthy donors per lot, the cutoff using integrated tests with normalization had the smaller deviation of positive rate between different reagent lots. Pregnant women had higher LA1/LA2 levels than nonpregnant women. Based on the results, normalization is needed because there are significant lot-to-lot variations. Integrated tests with normalization might be a better standard by which to confirm lupus anticoagulant. Pregnant women should have population-specific cutoffs because they have higher LA1/LA2 levels.
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http://dx.doi.org/10.1177/10760296211048897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521729PMC
October 2021

The correlation of the miR-29a/MMP9 axis with infection in gastric cancer.

Am J Transl Res 2021 15;13(9):10155-10162. Epub 2021 Sep 15.

The Sixth People's Hospital of Jinan Jinan, Shandong Province, China.

Objective: This study was designed to investigate the association between the miR-29a/MMP9 axis expression levels and (HP) infection in gastric cancer patients.

Methods: A total of 100 gastric cancer patients referred to our hospital from June 2017 to June 2019 were recruited as the study cohort. Among them, 50 HP-positive patients were included in the experimental group and 50 HP-negative patients were included in the control group. The changes in the patients' conditions were compared, the miR-29a/MMP9 axis expression levels were recorded, and the correlation between the miR-29a/MMP9 axis and the HP infections was analyzed. All the discharged patients were followed up for one year to analyze the correlation between the HP infections and the serum miR-29a and MMP9 expression levels with the disease progression.

Results: The experimental group had higher miR-29a expression levels and higher MMP9 chromogenic scores than the control group (P<0.05). A negative correlation was found between the miR-29 expression level and the MMP9 expression level (r=-5.369, P<0.05). One year after discharge, there were 27 patients with severe disease in the experimental group and 6 in the control group, with a significant difference between the two groups. Moreover, the expression levels of the miR-29a/MMP9 axis were significantly higher in the discharged patients than in the patients with severe disease (P<0.05). A receiver operating characteristic (ROC) curve was used to analyze the predictive value of miR-29/MMP9 in the diagnosis of gastric cancer, and the area under the curve was found to be 0.97.

Conclusion: The miR-29a/MMP9 axis levels were increased in the HP positive patients but not in the HP negative patients. HP infection is considered to be closely related to gastric cancer cell spread, disease relapse, and high miR-29a/MMP9 axis expression levels.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506987PMC
September 2021

Synergistic function of Au NPs/GeO nanozymes with enhanced peroxidase-like activity and SERS effect to detect choline iodide.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Oct 6;266:120467. Epub 2021 Oct 6.

Key Laboratory of Infrared Imaging Materials and Detector, Shanghai Institute of Technical Physics, Chinese Academy of Science, Shanghai 200083, China.

A novel Au NPs/GeO nanozymes are developed as Surface-Enhanced Raman Scattering (SERS) substrates with the promising prospect for detection ChI. Herein, it is discovered that both Au NPs and GeO nanozymes have peroxidase-like activity, catalyzing colorless 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue TMB. Interestingly, compared with single Au NPs or GeO nanozymes, the Au NPs/GeO nanozymes show stronger peroxidase-like activity, and significantly ameliorated SERS signal of TMB. The mentioned two enhancements are ascribed to a positive synergistic function of Au NPs/GeO nanozymes. Surprisingly, choline iodide (ChI) can inhibit the positive synergy in Au NPs/GeO nanozymes, and slow down the reaction of TMB-HO-Au NPs/GeO system. On this foundation, a new Au NPs/GeO SERS technique with high sensitivity, label-free detection method of choline iodide (ChI) is established, suggesting that Au NPs/GeO nanozymes have the potential application of water environment.
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http://dx.doi.org/10.1016/j.saa.2021.120467DOI Listing
October 2021

Comparative transcriptomic analysis of THP-1-derived macrophages infected with Mycobacterium tuberculosis H37Rv, H37Ra and BCG.

J Cell Mol Med 2021 Oct 10. Epub 2021 Oct 10.

State Key Laboratory of Analytical Chemistry for Life Science, Medical School of Nanjing University, Nanjing, China.

Tuberculosis (TB) remains a worldwide healthcare concern, and the exploration of the host-pathogen interaction is essential to develop therapeutic modalities and strategies to control Mycobacterium tuberculosis (M.tb). In this study, RNA sequencing (transcriptome sequencing) was employed to investigate the global transcriptome changes in the macrophages during the different strains of M.tb infection. THP-1 cells derived from macrophages were exposed to the virulent M.tb strain H37Rv (Rv) or the avirulent M.tb strain H37Ra (Ra), and the M.tb BCG vaccine strain was used as a control. The cDNA libraries were prepared from M.tb-infected macrophages and then sequenced. To assess the transcriptional differences between the expressed genes, the bioinformatics analysis was performed using a standard pipeline of quality control, reference mapping, differential expression analysis, protein-protein interaction (PPI) networks, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Q-PCR and Western blot assays were also performed to validate the data. Our findings indicated that, when compared to BCG or M.tb H37Ra infection, the transcriptome analysis identified 66 differentially expressed genes in the M.tb H37Rv-infected macrophages, out of which 36 genes were up-regulated, and 30 genes were down-regulated. The up-regulated genes were associated with immune response regulation, chemokine secretion, and leucocyte chemotaxis. In contrast, the down-regulated genes were associated with amino acid biosynthetic and energy metabolism, connective tissue development and extracellular matrix organization. The Q-PCR and Western blot assays confirmed increased expression of pro-inflammatory factors, altered energy metabolic processes, enhanced activation of pro-inflammatory signalling pathways and increased pyroptosis in H37Rv-infected macrophage. Overall, our RNA sequencing-based transcriptome study successfully identified a comprehensive, in-depth gene expression/regulation profile in M.tb-infected macrophages. The results demonstrated that virulent M.tb strain H37Rv infection triggers a more severe inflammatory immune response associated with increased tissue damage, which helps in understanding the host-pathogen interaction dynamics and pathogenesis features in different strains of M.tb infection.
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http://dx.doi.org/10.1111/jcmm.16980DOI Listing
October 2021

Reasonable Timing of Radiotherapy for Stage IV Non-Small-Cell Lung Cancer During Targeted Therapy Based on Tumour Volume Change.

Front Oncol 2021 23;11:705303. Epub 2021 Sep 23.

Department of Oncology, Affiliated Hospital of Guizhou Medical University, Guiyang, China.

Purpose: The aim of this study was to investigate the reasonable timing of radiotherapy for stage IV non-small-cell lung cancer (NSCLC) with EGFR-positive mutations during targeted therapy based on tumour volume change (TVC).

Patients And Methods: Simulation Computed Tomography Scan (SCTS) measurements were taken to test TVC in patients with stage IV NSCLC during targeted therapy at intervals of 10 days. The SCTS measurement was terminated when the tumour volume shrinkage rate in the latter simulation compared with the previous simulation was ≤5% or when the time after treatment was 90 days. Then, primary tumour radiotherapy was performed. Related parameters of the radiotherapy plan were compared between the implementation and simulation plans.

Results: Twenty-seven patients were enrolled in the analysis. After treatment, shrinkage of the primary tumour was observed in all patients, but the rate and speed were inconsistent. The average tumour volume decreased obviously within 40 days and was significantly different every 10 days (P ≤ 0.001). The average volume decreased slowly and tended to be stable (P>0.05) after 40 days. After the termination of SCTSs, 21 patients accepted primary tumour radiotherapy. No patients experienced grade 3+ acute radiation toxicity. The implementation radiotherapy plan was significantly better than that before treatment (all P<0.05) but not better than that on the 40th day after treatment (all P>0.05).

Conclusions: To obtain a high radiation dose and control radiation toxicity, the 40th day after targeted therapy may be a reasonable time to start radiotherapy for stage IV NSCLC with EGFR-positive mutations.

Clinical Trial Registration: https://www.clinicaltrials.gov/ct2/show/NCT03258671, identifier, NCT03258671.
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http://dx.doi.org/10.3389/fonc.2021.705303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496348PMC
September 2021

Values of Contrast-Enhanced Ultrasound in Classification and Diagnosis of Common Bile Duct and Superficial Organ Lesions under Compression Algorithm.

J Healthc Eng 2021 29;2021:9577440. Epub 2021 Sep 29.

Department of Ultrasound, Minzu Hospital of Guangxi Zhuang Autonomous Region, Affiliated Minzu Hospital of Guangxi Medical University, Nanning 530001, China.

This work aimed to investigate values of contrast-enhanced ultrasound (CEUS) under DEFLATE in the classification and diagnosis of the common bile duct and superficial lymphoid lesions. 88 patients with lower common bile duct lesions and 126 patients with superficial lymphoid lesions were selected as the subjects investigated and examined by CEUS under DEFLATE to compare characteristics and diagnostic efficiency of CEUS in different types of lesions. The time-intensity curve (TIC) was for quantitative analysis on CEUS results. The results showed that there were statistically significant differences in the comparison of time to peak (TTP), area under the curve (AUC), and gradient (Grad) of common bile duct walls in patients from the malignant group ( < 0.05), while the comparison of three indicators of patients in the benign group was not statistically remarkable ( > 0.05). In addition, there were statistically great differences in TTP, AUC, and Grad among patients in the benign and malignant groups ( < 0.05). The sensitivity, specificity, accuracy, and positive/negative predictive value of CEUS + ultrasound (US) in the diagnosis of benign and malignant lymph nodes were 92.83%, 87.14%, 89.54%, 91.23%, and 86.43%, respectively. The values of maximal intensity ( ) in the reactive hyperplasia group (group A), lymphoma group (group B), and metastatic lymph nodes group (group C) were compared, showing statistical differences ( < 0.05). The TTP and AUC of group B were higher than those of groups A and C, respectively ( < 0.05), and the base-to-peak ascending slope ( ) and the absolute value of the semidescending slope ( ) in group C increased hugely compared to group A ( < 0.05). It indicated that CEUS examination under DEFLATE could be applied in the qualitative diagnosis of lower common bile duct lesions and superficial lymphoid lesions, which was worthy of clinical application.
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http://dx.doi.org/10.1155/2021/9577440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494555PMC
September 2021

Competing Nomogram for Late-Period Breast Cancer-Specific Death in Patients with Early-Stage Hormone Receptor-Positive Breast Cancer.

Clin Breast Cancer 2021 Aug 31. Epub 2021 Aug 31.

Department of Medical Oncology, Jinhua hospital of Zhejiang University, Jinhua , Zhejiang Province, China.

Background: More than half of early breast cancer recurrences occur after 5 years from the initial diagnosis. An individualized estimate of the risk of late-period breast cancer-specific death (LP-BCSD) after 5 years of endocrine therapy (ET) can improve decision-making for extended endocrine therapy (EET).

Materials And Methods: A total of 147,059 eligible patients with breast cancer who survived 5+ years after diagnosis between 1990 and 2007 were identified from the Surveillance, Epidemiology, and End Results (SEER) database. Univariate and multivariate analyses based on the competing risk regression model were used to evaluate predictive factors for high risk of LP-BCSD or late-period non-breast cancer-specific death (LP-non-BCSD). Significant factors were used to build a nomogram to individualize estimates of LP-BCSD or LP-non-BCSD.

Results: The 5- and 10-year LP-BCSD rates were 5.7% and 10.1%, respectively, and the 5- and 10-year LP-non-BCSD rates were 6.7% and 15.5%, respectively. Young age, black race, single marital status, poor differentiation, large tumor size, lymph node metastasis, and estrogen receptor-positive/progesterone receptor-negative (ER+/PR-) status were independent predictive factors for high risk of LP-BCSD. Age was the most important factor for predicting high risk of LP-non-BCSD. The nomograms, which were based on significant factors identified by the competing risk regression model. A risk score system based on the competing risk nomogram was established to describe the relative risk of LP-BCSD and LP-non-BCSD.

Conclusion: This study explored the novel endpoint of LP-BCSD for further clinical trials. The risk score system might be highly useful for patient counseling, especially in discussing EET options with elderly or comorbid patients.
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http://dx.doi.org/10.1016/j.clbc.2021.08.008DOI Listing
August 2021

Accuracy of ultrasonography in diagnosis of fetal central nervous system malformation.

World J Clin Cases 2021 Sep;9(27):8027-8034

Department of Ultrasound, The First Affiliated Hospital of Wannan Medical College, Wuhu 241000, Anhui Province, China.

Background: Prenatal examination is an important measure for the screening and diagnosis of fetal malformations.

Aim: To investigate the accuracy of ultrasonography in the diagnosis of fetal central nervous system (CNS) malformations.

Methods: One hundred and thirteen pregnant women suspected of having fetal CNS malformations were examined at our hospital from December 2018 to October 2020 using two-dimensional ultrasonography and three-dimensional ultrasonography, respectively.

Results: According to the pathological results, there were 79 cases of CNS malformations and 34 cases of non-CNS malformations among the 113 pregnant women suspected of having fetal CNS malformation. Fifty-one cases of CNS malformation and 26 cases of non-CNS malformation were detected by two-dimensional ultrasonography, and 73 cases of CNS malformation and 30 cases of non-CNS malformation were detected by three-dimensional ultrasonography. The diagnostic sensitivity (92.41%) and accuracy (91.15%) of three-dimensional ultrasonography were higher than those of two-dimensional ultrasonography (64.56% and 68.14%, respectively) ( = 0.000). The specificity of three-dimensional ultrasonography (88.24%) was higher than that of two-dimensional ultrasonography (76.47%); however, the difference was not significant ( = 0.203).

Conclusion: Three-dimensional ultrasonography has high application value in the diagnosis of fetal CNS malformations. In addition, the image quality is clear, and the diagnostic sensitivity and accuracy are high.
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http://dx.doi.org/10.12998/wjcc.v9.i27.8027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8462195PMC
September 2021

Low-dose IL-2 therapy invigorates CD8+ T cells for viral control in systemic lupus erythematosus.

PLoS Pathog 2021 Oct 7;17(10):e1009858. Epub 2021 Oct 7.

Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135), Beijing, China.

Autoimmune diseases are often treated by glucocorticoids and immunosuppressive drugs that could increase the risk for infection, which in turn deteriorate disease and cause mortality. Low-dose IL-2 (Ld-IL2) therapy emerges as a new treatment for a wide range of autoimmune diseases. To examine its influence on infection, we retrospectively studied 665 patients with systemic lupus erythematosus (SLE) including about one third receiving Ld-IL2 therapy, where Ld-IL2 therapy was found beneficial in reducing the incidence of infections. In line with this clinical observation, IL-2 treatment accelerated viral clearance in mice infected with influenza A virus or lymphocytic choriomeningitis virus (LCMV). Noticeably, despite enhancing anti-viral immunity in LCMV infection, IL-2 treatment exacerbated CD8+ T cell-mediated immunopathology. In summary, Ld-IL2 therapy reduced the risk of infections in SLE patients and enhanced the control of viral infection, but caution should be taken to avoid potential CD8+ T cell-mediated immunopathology.
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http://dx.doi.org/10.1371/journal.ppat.1009858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525737PMC
October 2021

Low-Dose Interleukin-2 as an Alternative Therapy for Refractory Lupus Nephritis.

Rheumatol Ther 2021 Oct 7. Epub 2021 Oct 7.

Department of Rheumatology and Immunology, Peking University People's Hospital, 11 Xizhimen South street, Beijing, 100044, China.

Introduction: Low-dose interleukin-2 (IL-2) selectively restores disturbances of regulatory T cells (Treg) and conventional T cells, resulting in the induction of remission in patients with systemic lupus erythematosus. However, to date no research has been carried out on the efficacy of low-dose IL-2 in the treatment of refractory lupus nephritis (LN). The aim of the study reported here was to investigate the renal response to low-dose IL-2 in patients with refractory LN.

Methods: The study population comprised ten patients with refractory LN who failed to achieve complete response or who had relapsed while being treated with at least two conventional immunosuppressive agents. One treatment cycle consisted of IL-2 at a dose of 1 million IU administered subcutaneously every other day for 2 weeks followed by a 2-week break. All patients received three cycles of IL-2 and were then followed up for another 12 weeks without any increase in the dose of previous immunosuppressive agents and steroids.

Results: Of the ten patients enrolled in the study, seven (70%) achieved ≥ 50% improvement in proteinuria at 12 weeks after initiating treatment with IL-2. Median proteinuria was significantly reduced by 50.3% at week 12, from 1.83 (interquartile range [IQR] 1.23-3.21)  g/24 h at baseline to 0.91 (IQR 0.52-1.60)  g/24 h at 12 weeks (P = 0.005). This was accompanied by a 71% reduction in urine erythrocytes, from 64/µl (IQR 24-102/µl) at baseline to 18/µl (IQR 2-20/µl) at 12 weeks (P = 0.018). Anti-dsDNA was decreased from 27.9 (IQR 7.6-40.28) IU/ml at baseline to 14.1 (IQR 7.3-20.12) IU/ml (P = 0.021) at week 12, while complements C3 and C4 were slightly increased (P = 0.445, P = 0.241, respectively). A significant expansion of Treg cells, from 9.3% at baseline to 16.6% at 12 weeks, was also found (P < 0.05). No serious adverse events occurred during the treatment period.

Conclusions: Low-dose IL-2 therapy may have a promising role in the treatment of refractory LN as an alternative and safe therapeutic approach. It may be used as multi-target combination therapy in clinical practice.
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http://dx.doi.org/10.1007/s40744-021-00381-1DOI Listing
October 2021

Study on the Mechanism of treating COVID-19 with Shenqi Wan based on Network Pharmacology.

Drug Dev Ind Pharm 2021 Oct 4:1-22. Epub 2021 Oct 4.

School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.

Purpose: Through the method of network pharmacology, the active components and targets of Shenqi wan (SQW) were excavated, the relationship with COVID-19 was discussed, and the possible mechanism of SQW in the treatment of COVID-19 was revealed from the aspects of multi-components, multi-targets, and multi-pathways.

Methods: Firstly, the active components of SQW were screened from TCMSP and the 2020 edition of Chinese Pharmacopoeia, and the related targets of the components were obtained. Then the disease targets related to COVID-19 were screened from GeneCards and OMIM. Venny was used to map the relationship between component-target and disease-target, and String was used to analyzing the interaction of common targets. The network was constructed and analyzed by Cytoscape, the function of GO and KEGG genes was enriched by Metascape, and the molecular docking was verified by CB-Dock.

Results: Finally, 45 active components of SQW were obtained, and 72 potential targets were related to COVID-19, ACE2, IL6, NOS3, and CRP may be the key targets. GO enrichment of 1715 projects, such as lipopolysaccharide stress response, active oxygen metabolism, positive regulation of cell migration, and other GO enrichment. 136 KEGG pathways were obtained, TNF signaling pathway, IL-17 signaling pathway, HIF-1 signaling pathway. Molecular docking showed that kaempferol, quercetin, luteolin, astragaloside, calyx isoflavone glucoside, matrine, and other COVID-19-related targets such as ACE2, 3CLpro, PLpro, PTGS2 have good binding ability.

Conclusion: According to the above results, it is suggested that SQW may play a role in the treatment of COVID-19 by directly or indirectly combining kaempferol, quercetin, and luteolin with ACE2, 3CLpro, PLpro, and PTGS2 to regulate multiple biological functions and signaling pathways.
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http://dx.doi.org/10.1080/03639045.2021.1989453DOI Listing
October 2021

Analysis of Genomic Copy Number Variation in Miscarriages During Early and Middle Pregnancy.

Front Genet 2021 17;12:732419. Epub 2021 Sep 17.

Center for Precision Medicine, Meizhou People's Hospital (Huangtang Hospital), Meizhou Academy of Medical Sciences, Meizhou, China.

The purpose of this study was to explore the copy number variations (CNVs) associated with miscarriage during early and middle pregnancy and provide useful genetic guidance for pregnancy and prenatal diagnosis. A total of 505 fetal specimens were collected and CNV sequencing (CNV-seq) analysis was performed to determine the types and clinical significance of CNVs, and relevant medical records were collected. The chromosomal abnormality rate was 54.3% (274/505), among which the numerical chromosomal abnormality rate was 40.0% (202/505) and structural chromosomal abnormality rate was 14.3% (72/505). Chromosomal monosomy mainly occurred on sex chromosomes, and chromosomal trisomy mainly occurred on chromosomes 16, 22, 21, 15, 13, and 9. The incidence of numerical chromosomal abnormalities in ≥35 year-old age pregnant women was significantly higher than <35 year-old age group. The highest incidence of pathogenic CNV (pCNV) was found in fetuses at ≤6 weeks of pregnancy (5.26%), and the incidence of variants of unknown significance (VOUS) CNVs decreased gradually with the increase of gestational age. The rate of chromosomal abnormalities of fetuses in early pregnancy (59.5%) was higher than that of fetuses in middle pregnancy (27.2%) ( < 0.001). There were 168 genes in VOUS + pCNV regions. 41 functions and 12 pathways ( < 0.05) were enriched of these genes by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Some meaningful genetic etiology information such as genes and pathways has been obtained, it may provide useful genetic guidance for pregnancy and prenatal diagnosis.
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http://dx.doi.org/10.3389/fgene.2021.732419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484914PMC
September 2021

Dampening Effects of Perceived Teacher Enthusiasm on Class-Related Boredom in College Students: Longitudinal Mediation Effects of Perceived Task Value.

Front Psychol 2021 16;12:712441. Epub 2021 Sep 16.

Department of Students' Affairs, Wenzhou University of Technology, Wenzhou, China.

This study aims to explore the longitudinal mediation effects of college students' perceived task value (PTV) between perceived teacher enthusiasm (PTE) and class-related boredom (CB). We conducted a longitudinal survey among college students from five colleges at the second (T1), sixth (T2), tenth (T3), and fourteenth week (T4) in a semester, and overall 1,371 students completed all the measurements. In the survey, a battery of questionnaires was used to measure the students' PTE, perception of task difficulty, perception of task value, and CB. At T1, boredom proneness was measured as a control variable. Analysis of the longitudinal data showed that after controlling for the effects of boredom proneness and perceived task difficulty, students' PTE was a significant predictor of CB, and students' PTV played a significant mediating role in this causation relationship. The study supported the importance of the control-value theory in explaining the mitigating effect of students' PTE on CB, especially highlighting the role of PTV.
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http://dx.doi.org/10.3389/fpsyg.2021.712441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481690PMC
September 2021

Clinical assessment of cardiac impairment favored by two-dimensional speckle tracking echocardiology in patients with systemic sclerosis.

Rheumatology (Oxford) 2021 Oct 2. Epub 2021 Oct 2.

Departments of Rheumatology, and Immunology, People's Hospital, Peking University, Beijing, China.

Background: Cardiac involvement is a major cause of death in systemic sclerosis (SSc), while early detection remains a challenge.

Objectives: The purpose of this study was to investigate the prevalence and clinical implications of cardiac impairment in SSc.

Methods: 95 consecutive SSc patients (55.6 ± 13.8 years old, 5.3 ± 8.1 years from diagnosis) were included in the study. Patients with heart diseases onset prior to SSc were excluded. All patients underwent two-dimensional speckle-tracking echocardiology (2D-STE) with measuring left and right ventricular global longitudinal strain (GLS/RGLS). Clinical manifestation, laboratory evaluation (CRP, cTnI, antibodies, etc.) and ECG were collected at the same time. Comparisons between the SSc subgroups (lcSSc and dcSSc) were performed using Student's t test, Mann-Whitney U or Fisher's exact test. Binary logistic regression was applied to determine the independent effects of variables in cardiac impairment.

Results: Early left and right ventricular impairment measured by GLS and RGLS were detected in 22.1% and 24.2% of the SSc patients, respectively. In comparison, only 2.1% showed reduced left ventricular ejection fraction (LVEF). Impaired GLS was mainly observed in the basal and medial segments of anterior, lateral, and posterior left ventricle walls, and more profound in dcSSc. Elevated CRP (OR 3.561 95% CI (1.071-11.839), p< 0.05) was associated with reduced GLS/RGLS. The adoption of GLS/RGLS enhanced the efficacy of routine screening for cardiac impairment that 52.6% of patients showed potential cardiac impairment.

Conclusions: Cardiac impairment is a common manifestation in SSc. Increasing awareness of early cardiac impairment is warranted with elevated CRP and dcSSc.
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http://dx.doi.org/10.1093/rheumatology/keab724DOI Listing
October 2021

Quasi-Guided Modes in Titanium Dioxide Arrays Fabricated via Soft Nanoimprint Lithography.

ACS Appl Mater Interfaces 2021 Oct 30;13(40):47860-47870. Epub 2021 Sep 30.

School of Physics and CRANN, Trinity College Dublin, Dublin 2, Ireland.

Reversible quasi-guided modes (QGMs) are observed in titanium dioxide (TiO) metasurface arrays fabricated via soft nanoimprint lithography. A TiO layer between the nanopillar array and the substrate can facilitate the propagation of QGMs. This layer is porous, allowing for the tuning of the layer properties by incorporating another material. The presence of the QGMs is strongly dependent on the refractive index of the TiO layer. QGMs are not supported if the refractive index of the porous TiO is too low. It is demonstrated that after depositing R6G on the array QGMs can be observed as very strong and narrow reflectance peaks and transmittance dips. Furthermore, as the second material can penetrate through the pores into the layer it can experience the regions of high field enhancement associated with the QGMs. These results are of interest for a wide range of applications including but not limited to sensing, nonlinear optics, and emission control.
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http://dx.doi.org/10.1021/acsami.1c11456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517955PMC
October 2021

Neurotransmitters and Electrophysiological Changes Might Work as Biomarkers for Diagnosing Affective Disorders.

Dis Markers 2021 18;2021:9116502. Epub 2021 Sep 18.

Institute of Brain and Psychological Science, Sichuan Normal University, Chengdu, Sichuan 610000, China.

Affective disorders are the leading causes of human disability worldwide; however, the diagnosis is still hard to define, because emotion is the least study subjects in psychology. Recent emotional studies suggest that human emotions are developed from basic emotions, which are evolved for fundamental human lives. . In our previous papers, we suggested that there are three basic emotions: joy, fear, and disgust. . Further tests with event-related potentials (ERP) found that joy, fear, and disgust showed the fastest response compared with other emotions, suggesting that they are fast automatic responses, which confirmed that these three emotions are prototypical emotions. Other basic emotions, anger and sadness, are due to object induced behaviors instead of sensation of object, so they developed secondary to prototypical emotions. Thus, we concluded that only joy, fear, and disgust are prototypical emotions, which can mix into other emotions, like the primary colors. In all, the neural substrates for all emotions, including the affections, are possibly monoamine neuromodulators: joy-dopamine (peptides), fear (anger)-norepinephrine, and disgust-serotonin. We hope these basic emotional studies will offer some neural mechanisms for emotional processing and shed lights on the diagnosis of affective disorders.
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http://dx.doi.org/10.1155/2021/9116502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476281PMC
September 2021

First Report of Sclerotinia Blight on Peanut Caused by in Qinghai Province, China.

Plant Dis 2021 Sep 27. Epub 2021 Sep 27.

Shandong Peanut Research Institute , Plant Protect, No. 126 Wannianquan Road, Licang, Qingdao, Shandong, 266100, China, Qingdao, Shandong Province, China, 266100.

Historically, peanut has not been produced in Qinghai province located in Northwest China because of the high elevation and cold climates. However, since 2020 field studies have been conducted to evaluate peanut cultivars for suitability to field production. In 2020, peanut cultivation was successful for the first time in Haidong city, Qinghai province, China. In August 2020, brown, irregular-shaped lesions were observed on peanut stems from Qinghai province in China. In the early stage, the watersoaked spots were formed on the stems, then lesions expanded rapidly and became brown. In advanced stages of the disease, stems became bleached and eventually died. The inside of the stems was rotten and hollow, and the diseased stem wilted and died. White hyphae and black irregular shaped sclerotia were observed on the infected stems. Finally, local or whole plant rotted and died at the end. Approximately 10% of the plants in a field were infected. Symptomatic stems were cut into small pieces, disinfected with 75% ethanol for 1 minute, 0.5% NaClO for two minutes, and sterile water for three times. Pieces then were plated on potato dextrose agar (PDA) media and incubated at 25°C in darkness. Fungal colonies were initially white, becoming gray, then black sclerotia (2.4 to 6.0 mm in diameter) were appeared at the edge of colonies. Genomic DNA of the pure cultures of an isolate (ZHX7) was extracted and PCR was carried out using glyceraldehydes-3-phosphate dehydrogenase gene (G3PDH) region primers G3PDH-F/G3PDH-R, heat-shock protein 60 gene (HSP60) region primers HSP60-F/HSP60-R, and DNA-dependent RNA polymerase subunit gene (RPB2) region primers RPB2-F/RPB2-R (Staats et al., 2005), respectively. G3PDH region (Accession No. MZ388475) showed 99.44% sequence identity (887 bp out of 909 bp) to Sclerotinia sclerotiorum (Accession No. AJ705044, 887 bp out of 887 bp). HSP60 region (Accession No. MZ388476) showed 99.90% sequence identity (972 bp out of 984bp) to S. sclerotiorum (Accession No. AJ716048, 972 bp out of 980 bp). RPB2 region (Accession No. MZ388477) showed 100.00% sequence identity (1096 bp out of 1129 bp) to S. sclerotiorum (Accession No. AJ745716, 1096 bp out of 1096 bp). Phylogenetic analysis was done using Neighbor-Joining (NJ) analysis based on those gene sequences. The isolate was identified as S. sclerotiorum based on molecular analysis and morphological characteristics. For pathogenicity assay, ten-days-old potted peanut (Luhua No.12) seedlings were inoculated with one mycelial plug (8 mm in diameter ) by placing the inoculum on the base of the stem in a growth chamber (30°C in the day and 25°C at night, a 12-h photoperiod and 80% RH). All inoculated seedlings exhibited typical basal stem rot, and root showed different degrees of damage, and wilted 5 days after inoculation. No symptoms were observed on control plants treated with sterile distilled mycelial plugs, and S. sclerotiorum was consistently re-isolated from symptomatic tissue. S. sclerotiorum has been reported on peanut in Northeastern China (Yan et al., 2005). To our knowledge, this is the first report of S. sclerotiorum causing Sclerotinia Blight on peanut in Qinghai province, China. The peanut planting area in Qinghai has been further expanded this year, and S. sclerotiorum has a broad host range (Boland and Hall, 1994), so Sclerotinia Blight is a potential threat to peanut production, and as a result, it is critical for commercial producers to monitor plants for S. sclerotiorum.
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http://dx.doi.org/10.1094/PDIS-07-21-1503-PDNDOI Listing
September 2021

Dual-Frequency Polarized Reconfigurable Terahertz Antenna Based on Graphene Metasurface and TOPAS.

Micromachines (Basel) 2021 Sep 9;12(9). Epub 2021 Sep 9.

State Key Laboratory of Information Photonics and Optional Communications, Beijing University of Posts and Telecommunications, Beijing 100876, China.

A hybrid dual-frequency polarized reconfigurable terahertz antenna is designed and studied. Graphene and TOPAS are employed as the polarization conversion metasurface and dielectric substrate, respectively, enabling tunable polarization conversion and circular polarization. TOPAS is a good substrate material for broadband THz components due to its low absorption. By adjusting the chemical potential of graphene between 0 eV and 0.5 eV, the polarization state in the band of 1 THz (0.76-1.02 THz) and 2.5 THz (2.43-2.6 THz) can be reconstructed. Thanks to the multilayer graphene structure and low absorption TOPAS, the graphene metasurface exhibits a broad bandwidth of 0.26 and 0.17 THz, respectively, in the band of 1 THz and 2.5 THz. The working state of the circularly polarized antenna and linearly polarized antenna can be switched in the bands around 1 THz (0.7-0.75 THz, 0.96-1.04 THz) and 2.5 THz (2.42-2.52 THz), respectively, without changing the physical geometry. Moreover, the graphene antenna, metasurface, and hybrid structure are tested, respectively, to verify that the components do not interfere with each other in performance. The hybrid antenna shows great potential in tunable terahertz devices and related applications.
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http://dx.doi.org/10.3390/mi12091088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469653PMC
September 2021

[Anatomical study on reconstruction of calcaneus fibula ligament].

Zhongguo Gu Shang 2021 Sep;34(9):847-50

Department of Orthopaedics, the Third Affiliated Hospital of Wenzhou Medical College, Wenzhou 325200, Zhejiang, China.

Objective: To measure anatomical data of calcaneofibular ligament (CFL), relevant data of CFL attachment to provide an anatomical basis for CFL reconstruction.

Methods: Twenty-seven adult ankle specimens were selected, including 11 males and 16 females, aged from 22 to 71 years old with an average of (41.6±17.2) years old;9 cases on the left side and 18 cases on the right side. The specimens reserved at least 20 cm above ankle joint and a complete foot, and exclude deformities, fractures, incomplete development and degenerative lesions. CFL was performed detailed anatomical observation, morphological parameters of CFL was measured, and coordinates of fibula side and calcaneal side of CFL in the coordinate axis were measured. The distance between fibula insertion of CFL and fibula tip, distance between calcaneal insertion of CFL and lateral calcaneal nodule, and Angle between CFL and long axis of fibula were also measured.

Results: In these 27 specimens, CFL cases were all single bundles and the length of CFL was (32.83 ± 8.19) mm. The center point of fibula attachment in CFL was(2.87± 1.21) mm proximal with a coefficient of variation of 42.16% and (2.08±1.34) mm anteriorly with a coefficient of variation of 64.42%. The center point of calcaneal attachment region of CFL was located on coordinate axis on the distal end (15.32±5.33) mm, with a coefficient of variation of 34.79%, and the posterior part (6.38±2.15) mm, with a coefficient of variation of 33.86%. The distance between center point of fibula attachment and fibula tip was (4.81±0.82) mm. The distance between center point of calcaneal attachment area of CFL and lateral calcaneal nodules was(17.25±3.12) mm. Angle between CFL and fibula axis is (43 ±18)° .

Conclusion: According to anatomical studies, we could locate the fibula and calcaneal attachment of CFL by anatomical markers around ankle joint. However, the location of CFL attachment has a large variation, and the anatomical characteristics need to be considered in anatomical reconstruction.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.09.011DOI Listing
September 2021

Characterization and structural analysis of a thermophilic GH11 xylanase from compost metatranscriptome.

Appl Microbiol Biotechnol 2021 Oct 23;105(20):7757-7767. Epub 2021 Sep 23.

College of Science, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Xylanase is efficient for xylan degradation and widely applied in industries. We found a GH11 family xylanase (Xyn11A) with high thermostability and catalytic activity from compost metatranscriptome. This xylanase has the optimal reaction temperature at 80 °C with the activity of 2907.3 U/mg. The X-ray crystallographic structure shows a typical "right hand" architecture, which is the characteristics of the GH11 family enzymes. Comparing it with the mesophilic XYN II, a well-studied GH11 xylanase from Trichoderma reesei, Xyn11A is more compact with more H-bonds. Our mutagenic results show that the electrostatic interactions in the thumb and palm region of Xyn11A could result in its high thermostability and activity. Introducing a disulfide bond at the N-terminus further increased its optimal reaction temperature to 90 °C with augmented activity. KEY POINTS: • A hyperthermophilic xylanase with high activity was discovered using the metatranscriptomic method. • The mechanisms of thermophilicity and high activity were revealed using X-ray crystallography, mutagenesis, and molecular dynamics simulations. • The thermostability and activity were further improved by introducing a disulfide bond.
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http://dx.doi.org/10.1007/s00253-021-11587-2DOI Listing
October 2021

The Associations of Lymphocyte Ratio and Neutrophil Ratio on Liver Dysfunction in COVID-19 Patients.

Front Immunol 2021 6;12:717461. Epub 2021 Sep 6.

Department of Hepatology, Affiliated Hangzhou Xixi Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Data on the impact of lymphocytes and neutrophils on the incidence of liver dysfunction in COVID-19 patients are limited. This study aimed to investigate the lateral and longitudinal associations of lymphocyte ratio (LR) and neutrophil ratio (NR) on liver dysfunction in COVID-19 patients. We tested 1,409 blood samples from 245 COVID-19 patients in China between January 2020 and June 2021. The lateral U-shaped relationships, determined by smooth curve fitting and the piecewise-linear mixed-effect model, were observed between LR, NR, and AST and the incidence of AST-linked liver dysfunction, with the threshold cutoffs of 26.1 and 62.0, respectively. Over the 1,409 tests, the LR ≤ 26.1 and NR ≥ 62.0 related to the occurrence of mild liver dysfunction (HR: 1.36; 95% CI: 1.01, 1.82), moderate liver dysfunction (HR: 1.37; 95% CI: 1.01, 1.85), and severe liver dysfunction (HR: 1.72; 95% CI: 1.02, 2.90). For the patients with preexisting AST ≥ 35 U/L, the baseline LR ≤ 26.1 and NR ≥ 62.0 (b.LLCHN) groups had a fully adjusted 8.85-, 7.88-, and 5.97-fold increased risk of mild and moderate liver dysfunction after being hospitalized of 3, 6, and 9 days compared to the baseline LR > 26.1 and NR < 62.0 (b.normal) groups. Severe liver dysfunction only presents significant differences after being adjusted for age, sex, and BMI. Consistently, Kaplan-Meier analyses showed that b.LLCHN reflects a better predictive value for different subsequent magnitude liver dysfunctions after admission of 3 and 6 days. To improve liver function in patients with preexisting AST ≥35 U/L, future management strategies should pay more attention to baseline LR ≤ 26.1 and NR ≥ 62.0 patients.
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http://dx.doi.org/10.3389/fimmu.2021.717461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450365PMC
October 2021

Reaction Behaviors and Crystal Transformation of Industrial Vanadium-Phosphorus-Oxygen (VPO) Catalysts for -Butane Oxidation.

ACS Omega 2021 Sep 1;6(36):23558-23563. Epub 2021 Sep 1.

Dalian Research Institute of Petroleum and Petrochemicals, SINOPEC, Lvshun District, Dalian 116045, Liaoning, China.

A long-time evaluation of -butane oxidation over an industrial vanadium-phosphorus-oxygen (VPO) catalyst was implemented. The catalytic performances for -butane oxidation during this period were obtained. It was shown that the conversion of -butane increased with the evaluation time, but the selectivity of the maleic anhydride (MA) product decreased gradually. To investigate the crystal transformation of the VPO catalyst, the properties of fresh and evaluated VPO catalysts were measured by a series of characterization methods, including X-ray diffraction (XRD), N adsorption and desorption, NH-temperature programmed desorption (TPD), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). The results showed that the acidities and valence of vanadium increased after evaluation due to the appearance of a β-VOPO phase. The crystal transformation would increase the activity for -butane oxidation. Meanwhile, during the evaluation period, the decrease in selectivity of the MA product should be related to the decreasing percentage of Lat-O species in the VPO catalyst.
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http://dx.doi.org/10.1021/acsomega.1c03652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444314PMC
September 2021

Insight into sulfur dioxide and its derivatives metabolism in living system with visualized evidences via ultra-sensitive fluorescent probe.

J Hazard Mater 2021 Sep 11;423(Pt B):127179. Epub 2021 Sep 11.

CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China; School of Pharmacy, Binzhou Medical University, Yantai 264003, China. Electronic address:

Sulfur dioxide (SO) and its derivatives have long been considered as hazardous environmental pollutants but commonly used as food additives in safe dose range. They also could be produced from biological metabolism process of sulfur-containing amino acids. However, their physiological roles remain extremely obscure mainly due to lack of efficient tools for monitoring and imaging strategy establishment. Furthermore, most of current studies of this aspect focus on novel probe design or just imaging them rather than on the ins and outs. Therefore, there is a high significance of establishing highly sensitive detection strategy for monitoring SO derivatives in living systems, food and environment. Herein, we design a fluorescent probe MS-Bindol for sensitively detecting SO derivatives with a low detection limit (0.2 nM). We have established an imaging strategy for investigation of SO derivatives metabolism in living cells and zebrafish, providing visualize evidences and verified that SO derivatives could be synthetized from thiosulfate and glutathione(GSH) and be hardly consumed by using sulfite oxidase inhibitors (ferricyanide or arsenite). Moreover, the probe also exhibits excellent practicability in food as well as environmental samples. Our studies may help biologist for better understanding SO derivatives metabolism and deeply explore their physiological roles in biological systems.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127179DOI Listing
September 2021

Therapeutic effect of on defecation in senile constipation rat model through stem cell factor/C-kit signaling pathway.

World J Gastroenterol 2021 Aug;27(32):5392-5403

Department of Anorectal, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China.

Background: Constipation is one of the chronic gastrointestinal functional diseases. It seriously affects the quality of life. () can treat constipation obviously, but its mechanism has not been clarified. We supposed that mechanism of it improved the intestinal motility by stimulating interstitial Cajal cells (ICC). Activation of the C-kit receptor on the surface of ICC is closely related to ICC function, and the stem cell factor (SCF)/C-kit signaling pathways plays an important role on it. To investigate the mechanism of how treats constipation, this study aimed to establish a constipation model in rats and explore the role of SCF/C-kit signaling pathway in the treatment.

Aim: To explore the SCF/C-kit signaling pathways in the role of for treatment of constipation by a constipation rat model.

Methods: Forty-eight 8-mo-old Sprague-Dawley rats were divided into 4 groups by random weight method: Normal group ( = 12), model group ( = 12), group ( = 12) and blocker group ( = 12). The normal group received normal saline by gavage; the model group received loperamide by gavage; the blocker group received loperamide and by gavage, and STI571 was injected by intraperitoneally. During treatment, the weight, fecal granules and fecal quality were recorded every 10 d. On day 20 after model induction, the colon tissues of each group were removed. Hematoxylin and eosin staining was used to observe pathological changes. Expression levels of SCF, C-kit and genes were detected by immunohistochemistry, western blotting, and real-time-quantitative polymerase chain reaction. The colonic epithelial mitochondria and goblet cells were observed by transmission electron microscopy.

Results: Compared with the normal group, as treatment progressed, the weight of rats in the model and blocker groups decreased significantly, the stool weight decreased, and the stool quality was dry ( < 0.05). reversed the decrease in body weight and stool weight and improved stool quality. Histopathological analysis indicated that the colonic mucosal epithelium in the model group was incomplete, and the arrangement of the glands was irregular or damaged. Treatment with improved the integrity and continuity of the epithelial cells and regular arrangement of the glands. The blocking agents inhibited the effects of Immunohistochemistry and real-time-quantitative polymerase chain reaction showed that expression of SCF and C-kit protein or genes in the colonic tissue of the model group was decreased ( < 0.05), while treatment with increased protein or gene expression ( < 0.05). Western blotting showed that expression of aquaporin APQ3 was increased, while the expression of Cx43 decreased in the model group. Treatment with inhibited expression of APQ3 and promoted expression of Cx43. Transmission electron microscopy showed that the mitochondria of the colonic epithelium in the model group were swollen and arranged disorderly, and microvilli were sparse. The condition was better in the group. Mice treated with STI571 blocker confirmed that blocking the SCF/C-kit pathway inhibited the improvement of constipation by .

Conclusion: improved defecation in rats with constipation, and the SCF/C-kit signaling pathway, which is a key link of ICC function, played an important role of the treatment.
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http://dx.doi.org/10.3748/wjg.v27.i32.5392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409157PMC
August 2021

Nanosized CuO encapsulated Ni/Co bimetal Prussian blue with high anti-interference and stability for electrochemical non-enzymatic glucose detection.

Dalton Trans 2021 Oct 12;50(39):13748-13755. Epub 2021 Oct 12.

Department of Chemistry, College of Sciences, Northeastern University, Shenyang, Liaoning 110819, PR China.

Non-enzymatic glucose sensors based on metal oxides are receiving remarkable attention owing to their outstanding characteristics of being easy-to use, low cost, and reusability. However, the disadvantage of weak anti-interference associated with poor selectivity significantly restricts their applicability. Herein, we report a two-step fabrication of nanosized CuO encapsulated Ni/Co bimetal Prussian blue (PB) with a typical core-shell structure, which can be efficiently used for non-enzymatic glucose detection, ascribing to the permeability and abundant active sites of out-shelled crystalline porous Ni/Co PB and the high catalytic activity and conductivity of embedded CuO nanoparticles, afforded by their mutual synergistic interactions. The glassy carbon electrode modified with the hybrid of the CuO-encapsulated Ni/Co PB (simplified as the Ni/Co-PB/CuO/GCE electrode) exhibited a high glucose sensitivity of 600 μA mM cm with a low detection limit of 0.69 μM (S/N = 3), a fast response time (less than 3 s), and excellent long-term stability. In addition, the CuO-encapsulated Ni/Co PB showed favorable anti-interference ability in the presence of ascorbic acid (AA), L-lysine (Lys), dopamine (DA), cysteine (Cys), dopamine (DA), and KCl interferences. The reusability and long-term stability, as well as the practicability of the Ni/Co-PB/CuO/GCE sensing electrode verified by testing real serum samples were also investigated, and the experimental results demonstrated the applicability of the core-shell NiCo-PB/CuO based flexible electrochemical sensor for non-enzymatic glucose sensing in practical applications.
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http://dx.doi.org/10.1039/d1dt02361fDOI Listing
October 2021

Modulating the in vitro gastrointestinal digestibility of crystalline oil-in-water emulsion: Different fat crystal sizes and polymorphic forms under the same SFC.

Food Chem 2022 Jan 28;368:130723. Epub 2021 Jul 28.

School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China. Electronic address:

The effects of the fat crystal structure on lipid droplets digestion behaviors were investigated using an in vitro digestion model. The crystalline oil-in-water emulsions containing the same solid fat content (SFC) with different fat crystal sizes and polymorphic forms were fabricated by different storage protocols: constant-temperature and inconstant-temperature storage. Oral and gastric processing led to a significant increase (p < 0.05) in the d values of the two emulsions, and the two emulsions underwent partial coalescence and flocculation/aggregation. The free fatty acid (FFA) release profiles showed that the lipolysis extent decreased due to a larger crystal size. In addition, the two emulsions showed differences in beta polymorphism. This work further demonstrated that the FFA release could be modulated by the physical properties of the fat.
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http://dx.doi.org/10.1016/j.foodchem.2021.130723DOI Listing
January 2022
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