Publications by authors named "Xia Yang"

1,456 Publications

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Associations between long term exposures to outdoor air pollution and indoor solid fuel use and depression in China.

J Environ Manage 2021 Oct 23;302(Pt A):113982. Epub 2021 Oct 23.

Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, Heping District, Sanhao Street, No. 36, Shenyang, Liaoning Province, 110004, China. Electronic address:

Depression is one of the most common mental disorders. Effects of air pollution from outdoor and indoor on depression were inconsistent. We assessed 30,139 participants from Northeast China to explore the associations between long term exposures of outdoor and indoor solid fuel use and depressive symptoms. Multiple logistic regressions models as well as multiplicative interaction and additive interaction analysis were used. Outdoor exposures to air pollutants of particulate matter (with an aerodynamic diameter <2.5 μm, [PM], odds ratio [OR] = 1.98 per standard deviation [SD], 95% confidence interval [CI]: 1.78, 2.19; with an aerodynamic diameter <10 μm, [PM], OR = 1.83, 95% CI:1.68, 2.00), sulfur dioxide (SO, OR = 1.42, 95% CI: 1.33, 1.52), and nitrogen dioxide (NO, OR = 1.62, 95% CI: 1.49, 1.76) were significantly associated with higher occurrence of depressive symptoms. A significant linear trend for increased occurrence of depressive symptoms was observed in participants using both solid fuels for cooking and heating (P = 0.04). Indoor air pollution exposures from solid fuel use for heating (OR = 1.16, 95%CI: 1.00, 1.35) and high cooking frequency (OR = 1.17, 95%CI: 1.00, 1.37) were significantly associated with increased occurrence of depressive symptoms. We observed significant interactions of indoor solid fuel use and outdoor air pollution exposures on depressive symptoms (indoor fuel use for cooking and SO, P value = 0.04; solid fuel use for heating and NO, P value = 0.02). Solid fuel use for cooking weakened the associations between SO(relative excess risk due to interaction [RERI] = -1.37, 95% CI: -1.88, -0.86) and depressive symptoms. Solid fuel use for heating weakened the associations between NO (RERI = -1.91, 95% CI: -2.55, -1.27) and depressive symptoms. Compared with individual associations, antagonistic interactions of outdoor air pollution and indoor solid fuel use on depressive symptoms might exist. Our findings contribute to better understandings for the associations between air pollution and depressive symptoms, which might be useful for developing effective strategies for depression prevention and air pollution control.
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http://dx.doi.org/10.1016/j.jenvman.2021.113982DOI Listing
October 2021

Generation of Distal Renal Segments Involves a Unique Population of Aqp2+ Progenitor cells.

J Am Soc Nephrol 2021 Oct 19. Epub 2021 Oct 19.

W Zhang, Department of Regenerative & Cancer Cell Biology, Albany Medical College, Albany, United States.

See article for abstract.
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http://dx.doi.org/10.1681/ASN.2021030399DOI Listing
October 2021

Synergistic killing effects of PD-L1-CAR T cells and colorectal cancer stem cell-dendritic cell vaccine-sensitized T cells in ALDH1-positive colorectal cancer stem cells.

J Cancer 2021 13;12(22):6629-6639. Epub 2021 Sep 13.

The First People's Hospital of Hefei/The Third Affiliated Hospital of Anhui Medical University, Hefei 230061, P.R. China.

Cancer stem cells (CSCs) are characterized by self-renewal and unlimited proliferation, providing a basis for tumor occurrence, metastasis, and recurrence. Because CSCs are highly resistant to conventional chemotherapy and radiotherapy, various immunotherapies, particularly chimeric antigen receptor T cell (CAR-T) therapy and dendritic cell (DC)-based vaccine therapy, are currently being developed. Accordingly, in this study, we evaluated programmed cell death ligand-1 (PD-L1) expression in colorectal CSCs (CCSCs) and non-CCSCs and designed a combination immunotherapy synchronously utilizing PD-L1-CAR-T cells together with CCSC-DC vaccine-sensitized T cells for the treatment of colorectal cancer. PD-L1-CAR-T cells specifically recognized the PD-L1 molecule on CCSCs by binding to the extracellular domain of programmed cell death-1. The CCSC-DC vaccine was prepared using CCSC lysates. We found that aldehyde dehydrogenase 1 (ALDH1)-positive CCSCs were abundant in samples from patient tumor tissues and cancer cell lines. Moreover, PD-L1 was highly expressed in ALDH1-positive CCSCs compared with that in non-CCSCs. Monotherapy with PD-L1-CAR-T cells or CCSC-DC vaccine only elicited moderate tumor remission both and . However, combination therapy markedly killed cancer cells and relieved the tumor burden in mice. Our findings may provide a novel strategy for the clinical treatment of colorectal malignancy.
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http://dx.doi.org/10.7150/jca.62123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517999PMC
September 2021

MicroSPECT Imaging-Guided Treatment of Idiopathic Pulmonary Fibrosis in Mice with a Vimentin-Targeting Tc-Labeled -Acetylglucosamine-Polyethyleneimine.

Mol Pharm 2021 Oct 16. Epub 2021 Oct 16.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China.

Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease with poor prognosis. Evidence has shown that vimentin is a key regulator of lung fibrogenesis. Tc-labeled -acetylglucosamine-polyethyleneimine (NAG-PEI), a vimentin-targeting radiotracer, was used for the early diagnosis of IPF, and NAG-PEI was also used as a therapeutic small interfering RNA (siRNA) delivery vector for the treatment of IPF in this study. Single-photon emission-computed tomography (SPECT) imaging of bleomycin (BM)- and silica-induced IPF mice with Tc-labeled NAG-PEI was performed to visualize pulmonary fibrosis and monitor the treatment efficiency of siRNA-loaded NAG-PEI, lipopolysaccharide (LPS, a tolerogenic adjuvant), or zymosan (ZYM, an immunostimulant). The lung uptakes of Tc-NAG-PEI in the BM- and silica-induced IPF mice were clearly and directly correlated with IPF progression. The lung uptake of Tc-NAG-PEI in the NAG-PEI/TGF-β1-siRNA treatment group or LPS treatment group was evidently lower than that in the control group, while the lung uptake of Tc-NAG-PEI was significantly higher in the ZYM treatment group compared to that in the control group. These results demonstrate that NAG-PEI is a potent MicroSPECT imaging-guided theranostic platform for IPF diagnosis and therapy.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00545DOI Listing
October 2021

Chest CT imaging characteristics and their evolution of 48 patients with COVID-19 in Hengyang, China.

Am J Transl Res 2021 15;13(9):9983-9992. Epub 2021 Sep 15.

Department of General Practice, The Affiliated Nanhua Hospital, Hengyang Medical College, University of South China Hengyang 421002, Hunan, China.

The novel coronavirus 2019 (2019 nCoV), appeared in Wuhan in December 2019, can cause a novel coronavirus pneumonia (Corona Virus Disease 2019, COVID-19). COVID-19 is highly infectious and easy to infect people. The epidemic has gradually spread to all parts of the country. In order to provide a basis for clinical diagnosis, this study retrospectively analyzed the imaging characteristics, evolution and related imaging manifestations of COVID-19 patients in different stages of the disease. The results suggest that the imaging findings of 48 COVID-19 patients from Hengyang, Hunan Province are comparable in different stages of the disease. Chest CT showed no pneumonia in one mild patient. Chest CT findings of moderate type (n=38) and severe type (n=9) had comparable characteristics. The main manifestations were ground-glass opacity (GGO) (18/38, 47.37%; 1/9, 11.11%), and GGO with consolidation (16/38, 42.11%; 5/9, 55.56%), which respectively presented in bilateral lungs (34/38, 89.47%; 9/9, 100.00%), and multi-lobe distribution (involving 5 lobes) (17/38, 44.74%; 8/9, 88.89%). After treatment, 28 patients were isolated for 14 days and returned to the hospital for re-examination; among them, the pulmonary lesion was completely absorbed in 15 moderate patients, while 13 patients mainly manifested as GGO. The CT imaging findings of patients with COVID-19 can detect the lesions early, observe the scope of the lesions, evaluate the severity of the lesions, and assist the clinician in completing rapid isolation, diagnosis and treatment. At the same time, it can help to understand the performance of COVID-19 in different stages and dynamically detect changes in the patient's condition.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507075PMC
September 2021

Human Pluripotent Stem Cell-Derived Hepatocyte-Like Cells and Organoids for Liver Disease and Therapy.

Int J Mol Sci 2021 Sep 28;22(19). Epub 2021 Sep 28.

Division of Regenerative Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.

Liver disease is a global health issue that has caused an economic burden worldwide. Organ transplantation is the only effective therapy for end-stage liver disease; however, it has been hampered by a shortage of donors. Human pluripotent stem cells (hPSCs) have been widely used for studying liver biology and pathology as well as facilitating the development of alternative therapies. hPSCs can differentiate into multiple types of cells, which enables the generation of various models that can be applied to investigate and recapitulate a range of biological activities in vitro. Here, we summarize the recent development of hPSC-derived hepatocytes and their applications in disease modeling, cell therapy, and drug discovery. We also discuss the advantages and limitations of these applications and critical challenges for further development.
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http://dx.doi.org/10.3390/ijms221910471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508923PMC
September 2021

Evaluation of the prognostic ability of serum uric acid for elderly acute coronary syndrome patients with diabetes mellitus: a prospective cohort study.

J Zhejiang Univ Sci B 2021 Oct;22(10):856-865

Senior Department of Cardiology, the Sixth Medical Center of PLA General Hospital & Chinese PLA Medical School, Beijing 100853, China.

Objectives: This study evaluated the prognostic power of serum uric acid (UA) in predicting adverse events in elderly acute coronary syndrome (ACS) patients with diabetes mellitus (DM).

Methods: The analysis involved 718 ACS patients ‍>80 years old whose general clinical data and baseline blood biochemical indicators were collected prospectively from January 2006 to December 2012. These patients were classified into two groups based on DM status, and then followed up after discharge. The Kaplan-Meier method was used for major adverse cardiac event (MACE) rates and all-cause mortality. Multivariate Cox regression was performed to analyze the relationship between UA level and long-term clinical prognosis. Receiver operating characteristic (ROC) curves were analyzed to predict the cutoff value of UA in elderly ACS patients with DM. There were 242 and 476 patients in the DM and non-DM (NDM) groups, respectively, and the follow-up time after discharge was 40‒120 months (median, 63 months; interquartile range, 51‒74 months).

Results: The all-cause mortality, cardiac mortality, and MACE rates in both DM and NDM patients were higher than those in the control group (=0.001). All-cause mortalities, cardiac mortalities, and MACE rates in DM patients with moderate and high UA levels were significantly higher than those in the NDM group (=0.001). Long-term survival rates decreased significantly with increased UA levels in the ACS groups (=0.001). UA (odds ratio (OR)=2.106, 95% confidence interval (CI)=1.244‒3.568, =0.006) was found to be an independent risk factor for all-cause mortality and MACE in elderly ACS patients with DM. The cutoff value of UA was 353.6 μmol/L (sensitivity, 67.4%; specificity, 65.7%).

Conclusions: Serum UA level is a strong independent predictor of long-term all-cause death and MACE in elderly ACS patients with DM.
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http://dx.doi.org/10.1631/jzus.B2000637DOI Listing
October 2021

Congenital disorder of glycosylation caused by mutation of gene (c.1036G>A) in a Chinese infant: A case report.

World J Clin Cases 2021 Sep;9(26):7876-7885

Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.

Background: The gene coding for the accessory protein Ac45 of the vacuolar-type adenosine triphosphatases (V-ATPase) is located on chromosome Xq28. Defects in certain subunits or accessory subunits of the V-ATPase can lead to congenital disorders of glycosylation (CDG). CDG is a group of metabolic disorders in which defective protein and lipid glycosylation processes affect multiple tissues and organs. Therefore, the clinical presentation of patients with -CDG varies widely. In this report, we present a case of -CDG in a Chinese infant, with clinical features and genotype.

Case Summary: An 8-mo-old boy was admitted to our hospital because unexplained hepatosplenomegaly and elevated transaminases that had been noted while he was being treated for a cough at a local hospital. A post-admission examination at our hospital revealed abnormalities in the infant's liver, brain, and immune system. Trio-based whole exome gene analysis identified a hemizygous pathogenic mutation c.1036G>A (p.E346K) in exon 9 of the gene. This variant of the gene has not been reported in East Asian countries until now.

Conclusion: Based on the infant's clinical manifestations and the results of genetic detection, he was clearly diagnosed with -CDG. The clinical manifestations of children with CDG vary widely. Genetic testing analysis helps in the clinical diagnosis of children with CDG.
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http://dx.doi.org/10.12998/wjcc.v9.i26.7876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8462236PMC
September 2021

Associations of Nutritional, Lifestyle, and Metabolic Factors With Non-alcoholic Fatty Liver Disease: An Umbrella Review With More Than 380,000 Participants.

Front Nutr 2021 17;8:642509. Epub 2021 Sep 17.

Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, Shenyang, China.

Nonalcoholic fatty liver disease (NAFLD) is the most common liver injury. We performed this umbrella review of meta-analyses to summarize the evidence on the associations of nutritional, lifestyle, and metabolic factors with NAFLD. We searched the PubMed, Embase, and Web of Science databases from inception until July 2, 2020, to identify meta-analyses of observational studies which explored the associations of nutritional, lifestyle, and metabolic factors with NAFLD. Evidence levels were assessed using summary effect sizes, 95% prediction intervals, between-study heterogeneity, evidence of small-study effects, and evidence of excess significance bias for each meta-analysis. (No. of PROSPERO, CRD42020200124). Twenty two risk or protective factors from 10 published meta-analyses were included and studied. Three risk factors (sugar-sweetened beverage consumption, serum fetuin-A, and waist circumference) with highly suggestive levels of evidence and three risk factors (soft drink consumption, former smoking, and body mass index) with suggestive levels of evidence were identified. Only two protective factors (physical activity and serum vitamin D level [among adults in Western countries]) with suggestive levels of evidence were identified. Furthermore, other six risk factors and two protective factors with weak levels of evidence were identified. We found varying levels of evidence of associations of nutritional, lifestyle, and metabolic factors and NAFLD. The results suggest that nutritional and lifestyle management should be considered as a major primary preventive strategy for NAFLD. Moreover, considering the low quality of included meta-analyses and limited area of research topics, future high-quality original studies and meta-analyses should be performed to study these associations.
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http://dx.doi.org/10.3389/fnut.2021.642509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484322PMC
September 2021

Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.

PLoS One 2021 1;16(10):e0258159. Epub 2021 Oct 1.

Key Laboratory of State Forest Administration for Desert Ecosystem Protection and Restoration, College of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, China.

Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils. In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties. Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers. The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil. Compared with one-year sand barrier, lignin and cellulose of seven-year S. psammophila sand barrier decreased by 40.48% and 38.33%, respectively. Soil available potassium and available nitrogen increased by 39.80% and 99.46%, respectively. We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers. Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity. Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies. However, overall our findings improved the current understanding of the sand barrier-soil interactions on the process of ecological restoration.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0258159PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486110PMC
October 2021

PD-L1 heterogeneity in patients with non-small cell lung cancer.

Asia Pac J Clin Oncol 2021 Sep 28. Epub 2021 Sep 28.

Department of Oncology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Key Laboratory of Rheumatic Disease and Translational Medicine, Shandong Lung Cancer Institute, Jinan, Shandong Province, China.

Aims: Programmed death receptor ligand-1 (PD-L1) expression has been identified as the main predictor of responsiveness to programmed death receptor-1 (PD-1)-targeted immunotherapy in patients with advanced non-small cell lung cancer (NSCLC). In this retrospective study, we explored the inter- and intratumoral PD-L1 heterogeneity in patients who received two separate pathologically verified NSCLC diagnoses at Shandong Provincial Hospital Affiliated to Shandong First Medical University and Linyi People's Hospital.

Methods: Patients were classified into four groups according to the type of intra- and intertumoral heterogeneity: Group 1 included patients in whom primary tumor heterogeneity was observed between biopsy and surgical specimens; in Group 2, heterogeneity was observed between primary tumors and paired dissected regional lymph nodes; in Group 3, heterogeneity was observed between resected primary tumors and distant metastases or recurrent disease; in Group 4, heterogeneity was observed before and after microwave ablation. Of the 221 enrolled patients, 97, 36, 57, and 31 were allocated to groups 1, 2, 3, and 4, respectively.

Results: Significant heterogeneity was observed among all patients (P = 0.026) and within Group 1 (P = 0.022) when using a PD-L1 tumor proportion score (TPS) cut-off of 5%. However, no heterogeneity was observed in the other groups or with a PD-L1 TPS cut-off value of 1%.

Conclusions: Intratumoral PD-L1 expression heterogeneity between the biopsy and surgical specimens of primary tumors was more frequently detected than intertumoral heterogeneity in advanced NSCLC.
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http://dx.doi.org/10.1111/ajco.13605DOI Listing
September 2021

Measurement of the Total Lung Volume Using an Adjusted Single-Breath Helium Dilution Method in Patients With Obstructive Lung Disease.

Front Med (Lausanne) 2021 8;8:737360. Epub 2021 Sep 8.

Key Laboratory of Respiratory Disease of Zhejiang Province, Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China.

Whole-body plethysmography (WBP) is the gold standard for measuring lung volume, but its clinical application is limited as it requires expensive equipment and is not simple to use. Studies have shown that the single-breath helium dilution (SBHD) method, which is commonly used in clinical practice, significantly underestimates lung volume in patients with obstructive lung disease (OLD). By comparing the differences in lung volume measured using SBHD and WBP, we aimed to establish a correction equation for the SBHD method to determine the total lung volume in patients with OLD of different severities. From 628 patients with OLD simultaneously subjected to SBHD and WBP, 407 patients enrolled between January 2018 and November 2019 were in the training group and 221 enrolled between December 2019 and December 2020 were in the prospective verification cohort. The multiple linear regression equation was used for data in the training group to establish a correction equation for SBHD to determine the total lung volume, and this was validated in the prospective validation cohort. There was a moderate positive correlation between total lung capacity (TLC) determined using the SBHD [TLC (SBHD)] and WBP methods [TLC (WBP)] (r = 0.701; < 0.05), and the differences between TLC (SBHD) and TLC (WBP) (ΔTLC) were related to the severity of obstruction. As the severity of obstruction increased, the TLC was underestimated by the SBHD method. We established the following correction equation: () () = -0.669 + 0.756*()() - 0.047* +0.039* ()-0.009*()(r2 = 0.753 and adjusted r2 = 0.751). Next, we validated this equation in the validation cohort. With the correction equation, no statistical difference was observed between TLC (adjusted SBHD) and TLC (WBP) among the obstruction degree groups ( > 0.05). The SBHD method is correlated with WBP to measure the total lung volume, but the SBHD method presents limitations in determining the total lung volume in patients with obstructive lung disease. Here, we established an effective and reliable correction equation in order to accurately assess the total lung volume of patients with OLD using the SBHD method.
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http://dx.doi.org/10.3389/fmed.2021.737360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455942PMC
September 2021

IAPP-induced beta cell stress recapitulates the islet transcriptome in type 2 diabetes.

Diabetologia 2021 Sep 23. Epub 2021 Sep 23.

Larry L. Hillblom Islet Research Center, University of California, Los Angeles, David Geffen School of Medicine, Los Angeles, CA, USA.

Aims/hypothesis: Type 2 diabetes is characterised by islet amyloid and toxic oligomers of islet amyloid polypeptide (IAPP). We posed the questions, (1) does IAPP toxicity induce an islet response comparable to that in humans with type 2 diabetes, and if so, (2) what are the key transcriptional drivers of this response?

Methods: The islet transcriptome was evaluated in five groups of mice: beta cell specific transgenic for (1) human IAPP, (2) rodent IAPP, (3) human calpastatin, (4) human calpastatin and human IAPP, and (5) wild-type mice. RNA sequencing data was analysed by differential expression analysis and gene co-expression network analysis to establish the islet response to adaptation to an increased beta cell workload of soluble rodent IAPP, the islet response to increased expression of oligomeric human IAPP, and the extent to which the latter was rescued by suppression of calpain hyperactivation by calpastatin. Rank-rank hypergeometric overlap analysis was used to compare the transcriptome of islets from human or rodent IAPP transgenic mice vs humans with prediabetes or type 2 diabetes.

Results: The islet transcriptomes in humans with prediabetes and type 2 diabetes are remarkably similar. Beta cell overexpression of soluble rodent or oligomer-prone human IAPP induced changes in islet transcriptome present in prediabetes and type 2 diabetes, including decreased expression of genes that confer beta cell identity. Increased expression of human IAPP, but not rodent IAPP, induced islet inflammation present in prediabetes and type 2 diabetes in humans. Key mediators of the injury responses in islets transgenic for human IAPP or those from individuals with type 2 diabetes include STAT3, NF-κB, ESR1 and CTNNB1 by transcription factor analysis and COL3A1, NID1 and ZNF800 by gene regulatory network analysis.

Conclusions/interpretation: Beta cell injury mediated by IAPP is a plausible mechanism to contribute to islet inflammation and dedifferentiation in type 2 diabetes. Inhibition of IAPP toxicity is a potential therapeutic target in type 2 diabetes.
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http://dx.doi.org/10.1007/s00125-021-05569-2DOI Listing
September 2021

Thalidomide suppresses angiogenesis and immune evasion via lncRNA FGD5-AS1/miR-454-3p/ZEB1 axis-mediated VEGFA expression and PD-1/PD-L1 checkpoint in NSCLC.

Chem Biol Interact 2021 Nov 11;349:109652. Epub 2021 Sep 11.

Department of Radiotherapy, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu Province, China. Electronic address:

Background: Non-small cell lung cancer (NSCLC) accounts for about 80-85% of total lung cancer cases. Identifying the molecular mechanisms of anti-tumor drugs is essential for improving therapeutic effects. Herein, we aim to investigate the role of thalidomide in the tumorigenicity of NSCLC.

Methods: The A549 xenograft nude mouse model was established to explore therapeutic effects of thalidomide. The expression of FGD5-AS1 was evaluated in carcinomatous and paracarcinomatous tissues from NSCLC patients as well as NSCLC cell lines. CCK-8 assay was performed to assess cell viability. The invasive capacity was examined using transwell assay. The tube formation assay was applied to determine cell angiogenesis. Flow cytometry was subjected to validate CD8 T cell activity. The FGD5-AS1/miR-454-3p/ZEB1 regulatory network was analyzed using luciferase reporter, RIP and ChIP assays.

Results: Thalidomide reduced tumor growth and angiogenesis and increased CD8 T cell ratio in a mouse model. Enhanced expression of FGD5-AS1 was positively correlated with the poor survival of NSCLC patients. Knockdown of FGD5-AS1 notably suppressed the proliferation, invasion and angiogenesis of cancer cells as well as the apoptosis of CD8 T cells. Thalidomide targeted FGD5-AS1 to exert its anti-tumor activity in NSCLC. FGD5-AS1 acted as a sponge of miR-454-3p to upregulate ZEB1, thus increasing the expression of PD-L1 and VEGFA. Simultaneous overexpression of FGD5-AS1 and silencing of miR-454-3p reversed thalidomide-mediated anti-tumor effects in NSCLC.

Conclusion: Thalidomide inhibits NSCLC angiogenesis and immune evasion via FGD5-AS1/miR-454-3p/ZEB1 axis-mediated regulation of VEGFA expression and PD-1/PD-L1 checkpoint.
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http://dx.doi.org/10.1016/j.cbi.2021.109652DOI Listing
November 2021

Intestinal antiviral signaling is controlled by autophagy gene independent of the microbiota.

Autophagy 2021 Sep 14:1-16. Epub 2021 Sep 14.

Division of Infectious Diseases, Department of Medicine, Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.

Mutations in the macroautophagy/autophagy gene are responsible for Vici syndrome, a human genetic disease characterized by combined immunodeficiency. Previously, we found that mice exhibit hyperinflammation in the lungs mediated by IL1B/IL-1β and TNF/TNFα, resulting in resistance to influenza. Here, we find that disruption of results in protection against multiple enteric viruses including norovirus and rotavirus. Gene expression analysis reveals IFNL/IFN-λ responsive genes as a key alteration. Further, mice lacking exhibit substantial alterations of the intestinal microbiota. Surprisingly, germ-free mouse studies indicate associated inflammation of both the intestine and lung is microbiota-independent. Genetic studies support IFNL signaling as the primary mediator of resistance to enteric viruses, but not of microbial dysbiosis, in mice. This study unveils an important role, unexpectedly independent of the microbiota, for autophagy gene in host organism protection by modulating intestinal IFNL responses. CTNNB1: catenin (cadherin associated protein), beta 1; DAPI: 4',6-diamidino-2-phenylindole; EPG5: ectopic P-granules autophagy protein 5 homolog (C. elegans); FT: fecal transplant; IFI44: interferon-induced protein 44; IFIT1: interferon-induced protein with tetratricopeptide repeats 1; IFNG/IFN-γ: interferon gamma; IFNL/IFN-λ: interferon lambda; IFNLR1: interferon lambda receptor 1; IL1B/IL-1β: interleukin 1 beta; ISG: interferon stimulated gene; GF: germ-free; LEfSe: linear discriminant analysis effect size; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MNoV: murine norovirus; MX2: MX dynamin-like GTPase 2; OAS1A: 2'-5' oligoadenylate synthetase 1A; RV: rotavirus; SPF: specific-pathogen free; SQSTM1/p62: sequestosome 1; STAT1: signal transducer and activator of transcription 1; STING1: stimulator of interferon response cGAMP interactor 1; TBK1: TANK-binding kinase 1; TNF/TNFα: tumor necrosis factor.
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http://dx.doi.org/10.1080/15548627.2021.1968607DOI Listing
September 2021

Validity of the food frequency questionnaire for adults in nutritional epidemiological studies: A systematic review and meta-analysis.

Crit Rev Food Sci Nutr 2021 Sep 14:1-19. Epub 2021 Sep 14.

Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, Shenyang, China.

As the most widely used tool for assessing dietary intake, the validity of food frequency questionnaires (FFQs) should be evaluated before application. A comprehensive search of the PubMed and Web of Science databases was conducted for publications from January 2000 to April 1, 2020. Pooled estimates were calculated for correlation coefficients and mean differences for energy and 61 nutrients between FFQs and standard methods. The literature search identified 130 articles that included 21,494 participants. Subgroup analyses according to the number of administrations of the reference method, sample size, administration methods, FFQ items, reference periods, quality of the studies, gender, and regions were also performed. We conducted a meta-analysis by summarizing the available evidence to comprehensively assess the validity of FFQs stratified by the reference method type (24-hour recall (24HRs) and food records (FRs). We also performed subgroup analyses to examine the impact on the final summary estimates. After a meta-analysis of the FFQs' validity correlation coefficients of the included studies, this study showed that the range (median) of the validity coefficients of the 24HRs as reference methods was 0.220-0.770 (0.416), and for the FRs, it was 0.173-0.735 (0.373), which indicated that FFQs were suitable to assess the overall dietary intake in nutritional epidemiological studies. The results of the subgroup analysis showed that the number of administrations of the reference method, administration mode, number of items, reference periods, sample size, and gender mainly affected the validity correlation of FFQs.Supplemental data for this article is available online at https://doi.org/10.1080/10408398.2021.1966737 .
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http://dx.doi.org/10.1080/10408398.2021.1966737DOI Listing
September 2021

Indoor air pollution and frailty: A cross-sectional and follow-up study among older Chinese adults.

Environ Res 2021 Sep 6;204(Pt A):112006. Epub 2021 Sep 6.

Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, Shenyang, China. Electronic address:

Background: Previous studies have suggested that ambient air pollution negatively affects frailty, but whether indoor air pollution exposure affects frailty is unknown.

Method: This study was conducted on 4946 older adults (≥60 years) followed from baseline to 4 years in the Chinese Longitudinal Healthy Longevity Survey. Household fuel types and frailty were assessed with self-rated questionnaires and physical examination. The relationships between indoor air pollution and frailty via phenotypic frailty and a frailty index were explored with logistic regression models and Cox proportional hazard regression models in both a cross-sectional and follow-up design. Additionally, the effects of indoor air pollution on phenotypic frailty together with mild cognitive impairment (MCI) were further investigated.

Results: In the cross-sectional study, the adjusted ORs (95% CIs) for frailty assessment with the frailty index and phenotypic frailty were 1.28 (1.12, 1.46) and 1.36 (1.18, 1.57), respectively. Solid fuel use was a risk factor in prefrail/frail patients with [OR and 95% CI, 1.88 (1.41, 2.50)], or without MCI [OR and 95% CI, 1.37 (1.17, 1.61)], as compared with the groups with no phenotypic prefrailty/frailty and no MCI. Moreover, solid cooking fuel use was positively associated with the incidence of phenotypic prefrailty and frailty. The adjusted HRs (95% CIs) for phenotypic prefrailty and frailty were 1.26 (1.03, 1.55).

Conclusions: Solid cooking fuels can be regarded as a risk factor for frailty. Moreover, our findings suggest that more attention should be paid to solid cooking fuel using as it relates to phenotypic frailty together with MCI.
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http://dx.doi.org/10.1016/j.envres.2021.112006DOI Listing
September 2021

Establishment and validation of a computer-assisted colonic polyp localization system based on deep learning.

World J Gastroenterol 2021 Aug;27(31):5232-5246

Department of Gastroenterology, Changhai Hospital, Second Military Medical University/Naval Medical University, Shanghai 200433, China.

Background: Artificial intelligence in colonoscopy is an emerging field, and its application may help colonoscopists improve inspection quality and reduce the rate of missed polyps and adenomas. Several deep learning-based computer-assisted detection (CADe) techniques were established from small single-center datasets, and unrepresentative learning materials might confine their application and generalization in wide practice. Although CADes have been reported to identify polyps in colonoscopic images and videos in real time, their diagnostic performance deserves to be further validated in clinical practice.

Aim: To train and test a CADe based on multicenter high-quality images of polyps and preliminarily validate it in clinical colonoscopies.

Methods: With high-quality screening and labeling from 55 qualified colonoscopists, a dataset consisting of over 71000 images from 20 centers was used to train and test a deep learning-based CADe. In addition, the real-time diagnostic performance of CADe was tested frame by frame in 47 unaltered full-ranged videos that contained 86 histologically confirmed polyps. Finally, we conducted a self-controlled observational study to validate the diagnostic performance of CADe in real-world colonoscopy with the main outcome measure of polyps per colonoscopy in Changhai Hospital.

Results: The CADe was able to identify polyps in the test dataset with 95.0% sensitivity and 99.1% specificity. For colonoscopy videos, all 86 polyps were detected with 92.2% sensitivity and 93.6% specificity in frame-by-frame analysis. In the prospective validation, the sensitivity of CAD in identifying polyps was 98.4% (185/188). Folds, reflections of light and fecal fluid were the main causes of false positives in both the test dataset and clinical colonoscopies. Colonoscopists can detect more polyps (0.90 0.82, < 0.001) and adenomas (0.32 0.30, = 0.045) with the aid of CADe, particularly polyps < 5 mm and flat polyps (0.65 0.57, < 0.001; 0.74 0.67, = 0.001, respectively). However, high efficacy is not realized in colonoscopies with inadequate bowel preparation and withdrawal time ( = 0.32; = 0.16, respectively).

Conclusion: CADe is feasible in the clinical setting and might help endoscopists detect more polyps and adenomas, and further confirmation is warranted.
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http://dx.doi.org/10.3748/wjg.v27.i31.5232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384745PMC
August 2021

Tumor-derived exosomal miR-3157-3p promotes angiogenesis, vascular permeability and metastasis by targeting TIMP/KLF2 in non-small cell lung cancer.

Cell Death Dis 2021 09 8;12(9):840. Epub 2021 Sep 8.

Department of Thoracic Surgery, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.

Metastasis is the main cause of death in patients with advanced lung cancer. The exosomes released by cancer cells create tumor microenvironment, and then accelerate tumor metastasis. Cancer-derived exosomes are considered to be the main driving force for metastasis niche formation at foreign sites, but the mechanism in Non-small cell lung carcinoma (NSCLC) is unclear. In metastatic NSCLC patients, the expression level of miR-3157-3p in circulating exosomes was significantly higher than that of non-metastatic NSCLC patients. Here, we found that miR-3157-3p can be transferred from NSCLC cells to vascular endothelial cells through exosomes. Our work indicates that exosome miR-3157-3p is involved in the formation of pre-metastatic niche formation before tumor metastasis and may be used as a blood-based biomarker for NSCLC metastasis. Exosome miR-3157-3p has regulated the expression of VEGF/MMP2/MMP9 and occludin in endothelial cells by targeting TIMP/KLF2, thereby promoted angiogenesis and increased vascular permeability. In addition, exosome miR-3157-3p promoted the metastasis of NSCLC in vivo.
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http://dx.doi.org/10.1038/s41419-021-04037-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426367PMC
September 2021

Reference Values and Related Factors for Peak Expiratory Flow in Middle-Aged and Elderly Chinese.

Front Public Health 2021 20;9:706524. Epub 2021 Aug 20.

Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, Shenyang, China.

Peak expiratory flow (PEF), as an essential index used for screening and monitoring asthma, chronic obstructive pulmonary disease, and respiratory mortality especially in the elderly, is recommended for low-resource settings in low- and middle-income countries. However, few studies have focused on the reference of PEF in China, especially in middle-aged and elderly people. Thus, this study aimed to determine age- and sex-specific reference values of PEF in the middle-aged and elderly Chinese population. There were 8,914 participants who were included for risk factor analysis and 5,498 participants included for reference value analysis. The PEF was measured using a peak flow meter in liters per minute. The distributions of standardized PEF terciles stratified by sex and age were reported. Multiple linear regression analysis was used to determine the associations between risk factors and PEF. The PEF was higher in men than women across all age subgroups. The value of PEF decreased with age in both men and women. Height, weight, handgrip strength, and residence in rural were positively associated with PEF. Age and smoking status were negatively associated with PEF significantly in both men and women ( < 0.05). The mean PEF values were 367.10 and 253.00 L/min for men and women, respectively. Meanwhile, the prevalence of low PEF was 3.94 and 3.32% for men and women, respectively. Age- and sex-specific centiles of standardized PEF for the middle-aged and elderly Chinese population were estimated. The reference values for low PEF could provide reference standards for epidemiological studies and clinical practices in the future. Interventions to improve lung functions or to prevent respiratory disease should be paid more attention to factors associated with PEF.
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http://dx.doi.org/10.3389/fpubh.2021.706524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417711PMC
October 2021

Anisotropy of transverse and longitudinal relaxations in liquids entrapped in nano- and micro-cavities of a plant stem.

J Magn Reson 2021 Oct 18;331:107051. Epub 2021 Aug 18.

Physics Department, Oakland University, Rochester, MI, US.

We studied the anisotropy of H NMR spin-lattice and spin-spin relaxations in a fresh celery stem experimentally and modeled the sample theoretically as the water-containing nano- and micro-cavities. The angular dependence of the spin-lattice and the spin-spin relaxation times was obtained, which clearly shows the presence of water-filled nano- and micro-cavities in the celery stem, which have elongated shapes and are related to non-spherical vascular cells in the stem. To explain the experimental data, we applied the relaxation theory developed by us and used previously to interpret similar effects in liquids in nanocavities located in biological tissues such as cartilages and tendons. Good agreement between the experimental data and theoretical results was obtained by adjusting the fitting parameters. The obtained values of standard deviations (0.33 for the mean polar angle and 0.1 for the mean azimuthal angle) indicate a noticeable ordering of the water-filled nano- and micro-cavities in the celery stem. Our approach allows the use of the NMR technique to experimentally determine the order parameters of the microscopic vascular structures in plants.
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http://dx.doi.org/10.1016/j.jmr.2021.107051DOI Listing
October 2021

Genomic Landscape and Tumor Mutational Burden Determination of Circulating Tumor DNA in Over 5,000 Chinese Patients with Lung Cancer.

Clin Cancer Res 2021 Aug 26. Epub 2021 Aug 26.

Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Purpose: Having emerged as a noninvasive and clinically applicable approach for molecular determination of lung cancer, a genomic overview of circulating tumor DNA (ctDNA) of large-scale cohort may be helpful in novel biomarker development and therapeutic innovation.

Experimental Design: Primary cohort encompasses 5,671 blood samples from 4,892 patients with lung cancer. Pair-wise tissue samples from 579 patients and additional 358 sample pairs were collected to evaluate the correlation between blood and tissue tumor mutational burden (TMB). Parallel sequencing with plasma/tissue and white blood cells was performed using a 1,021-gene panel.

Results: Histologic subtyping was the most relevant to ctDNA detectability independent of other demographic characteristics, with small cell lung cancer showing the highest detectability, ctDNA abundance, and blood TMB (bTMB). Mutational landscape demonstrated significant differences, and integrated clonality analysis highlighted distinct driver-pattern and functional pathway interaction among various subtypes. The clonality and concurrent genes of mutations could predict the therapeutic efficacy of tyrosine kinase inhibitors (TKI), and mutations in non-small cell lung cancer characterized a subset with high bTMB, elevated ctDNA level, and potential small cell transformation. Most importantly, we developed an adjusted algorithm for bTMB in samples with extremely low ctDNA level and validated its correlation with tissue TMB in an independent cohort.

Conclusions: ctDNA could serve as a promising alternative in genomic profiling for lung cancer. The novel identification of ctDNA clonality and adjusted bTMB might improve therapeutic and prognostic evaluation. This dataset was also a valuable resource for the development of new therapeutic targets and new genomically guided clinical trials.
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http://dx.doi.org/10.1158/1078-0432.CCR-21-1537DOI Listing
August 2021

Atrazine Promoted Epithelial Ovarian Cancer Cells Proliferation and Metastasis by Inducing Low Dose Reactive Oxygen Species (ROS).

Iran J Biotechnol 2021 Apr 1;19(2):e2623. Epub 2021 Apr 1.

Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun 130041, China.

Background: Atrazine (ATZ) is a triazine herbicide that is widely used in agriculture and has been detected in surface and underground water. Recently, laboratory and epidemiological research have found that the bioaccumulation of ATZ in the environment leads to biotoxicity in the human immune and endocrine systems and results in tumor development.

Objective: To investigate the effects of ATZ exposure on epithelial ovarian cancer (EOC) cells and elucidate the potential mechanisms governing these effects.

Materials And Methods: The human EOC cell lines Skov3 and A2780 were used in this study to explore the effects and mechanisms of ATZ exposure on EOC. The mouse embryonic osteoblastic precursor MC3T3-E1 cells served as the control cells to determine the effects of ATZ on cancer cell lines. After exposure to ATZ, the MTT assay, flow cytometry, the colony formation assay, immunohistochemical staining, the cell scratch assay, and the Transwell assay were used to evaluate the proliferative activity, invasion, and migration capabilities of EOC cell lines. Moreover, flow cytometry was also applied to detect the level of reactive oxygen species (ROS) in these two EOC cell lines, as well as the MC3T3-E1 cells. To further illustrate the underlying mechanisms governing the effect of ATZ on EOC, real-time PCR and Western blotting were employed to assess the transcription and the expression level of Stat3 signaling pathway-related genes in Skov3 and MC3T3-E1 cells.

Results: The results showed that following ATZ treatment, the cell proliferation, migration, and invasion potencies of Skov3 and A2780 cells were increased compared to those of the control group. Meanwhile, the ROS levels of EOC and MC3T3-E1 cells were notably elevated after ATZ treatment. In Skov3 cells, the expression levels of p53 and p21 were downregulated, while those of Cyclin E, vascular endothelial growth factor (VEGF), matrix metallopeptidase 2 (MMP2), MMP9, signal transducers and activators of transcription 3 (Stat3), and p-Stat3 were upregulated by ATZ treatment. In MC3T3-E1 cells, however, ATZ treatment did not affect the level of p53/p21 mRNA compared to the control groups. Moreover, there was no significant change in the expression levels of Stat3 and p-Stat3 in MC3T3-E1 cells exposed to ATZ. This phenomenon was observed while the proliferation rate was enhanced in MC3T3-E1 cells by ATZ.

Conclusions: The results of this study suggest that ATZ effectively promotes the proliferation and metastasis of EOC cells through the Stat3 signaling pathway by inducing low levels of ROS. Additionally, although ATZ might also induce proliferative potential in normal cells, the mechanisms governing its effects in these cells might be different from those in EOC cells.
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http://dx.doi.org/10.30498/IJB.2021.2623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358173PMC
April 2021

Highly Efficient Spin-Filtering Transport in Chiral Hybrid Copper Halides.

Angew Chem Int Ed Engl 2021 Oct 1;60(44):23578-23583. Epub 2021 Oct 1.

Key Laboratory of Advanced Materials (MOE), School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, China.

Chiral Pb(Sn)-I hybrid organic-inorganic perovskites exhibit outstanding chiral-induced spin selectivity (CISS) performance, but the nontoxic lead-free hybrid materials with high stability are still greatly desired for spin filtering in spintronic applications. We synthesize chiral hybrid copper halides (R/S-MBA) CuX (MBA=methylbenzylammonium; X=Cl, Br) with characteristic 0D CuX tetrahedral structural motifs, combining the low toxicity of Cu and air stability of halide ions (Cl and Br ). Despite similar structural and electronic features, (R/S-MBA) CuBr shows much smaller chiroptical activity than the chloride counterpart. Magnetically conductive atomic force microscopy measurements display a typical spin-polarized charge-transport property with high efficiency up to 90 % for both copper halides. Our work expands the CISS effect into eco-friendly and stable metal-organic halides, which is promising for applications in spintronics based on transition-metal hybrid systems.
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http://dx.doi.org/10.1002/anie.202109595DOI Listing
October 2021

The association between water source and depressive symptoms in China: A cross-sectional and longitudinal study.

J Affect Disord 2021 Dec 31;295:56-62. Epub 2021 Jul 31.

Department of Clinical Epidemiology, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Shenyang, Liaoning, China, 110004; Clinical Research Center, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Shenyang, Liaoning, China, 110004. Electronic address:

Background: This study investigates the associations between residential water sources and symptoms of depression and the relationships between switching residential water sources and symptoms of depression in middle-aged and elderly people in China.

Methods: The cross-sectional study included 12,713 participants and the longitudinal study included 8,286 participants. Symptoms of depression were evaluated using the Center for Epidemiologic Studies Depression Scale. Multiple logistic and Cox regression analyses were used to identify associations between water sources and symptoms of depression.

Results: Participants without tap water were more likely to have depressive symptoms than participants with tap water (odds ratio (OR): 1.30; 95% confidence interval (CI): 1.19-1.42) (hazard ratio (HR): 1.18; 95% CI: 1.09-1.28). The lack of access to tap water in residence was positively related to the prevalence and incidence of symptoms of depression in almost all subgroups. Changing the water source from non-tap to tap water decreased the risk of depressive symptoms (HR: 0.69; 95% CI: 0.60 - 0.79).

Limitations: The results may not be generalizable to the whole population.

Conclusions: These results suggest that no tap water in residence is a risk factor for depressive symptoms. Therefore, providing tap water in residence may help prevent depression.
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http://dx.doi.org/10.1016/j.jad.2021.07.107DOI Listing
December 2021

Comparison and Mechanism Study of Antibacterial Activity of Cationic and Neutral Oligo-Thiophene-Ethynylene.

ACS Appl Mater Interfaces 2021 Sep 19;13(34):41012-41020. Epub 2021 Aug 19.

Institute of Nuclear Physics and Chemistry (INPC), China Academy of Engineering Physics (CAEP), Mianyang 621999, P. R. China.

Photodynamic therapy (PDT) is a potential approach to resolve antibiotic resistance, and phenylene/thiophene-ethynylene oligomers have been widely studied as effective antibacterial reagents. Oligomers with thiophene moieties usually exhibit good antibacterial activity under light irradiation and dark conditions. In the previous study, we verified that neutral oligo--phenylene-ethynylenes (OPEs) exhibit better antibacterial activity than the corresponding cationic ones; however, whether this regular pattern also operates in other kinds of oligomers such as oligo-thiophene-ethynylene (OTE) is unknown. Also, the antibacterial activity comparison of OTEs bearing cyclic and acyclic amino groups will offer useful information to further understand the role of amino groups in the antibacterial process and guide the antibacterial reagent design as amino groups affect the antibacterial activity a lot. We synthesized four OTEs bearing neutral or cationic, cyclic, or acyclic amino groups and studied their antibacterial activity in detail. The experimental results indicated that the OTEs exhibited better antibacterial activity than the OPEs, the neutral OTEs exhibited better antibacterial activity in most cases, and OTEs bearing cyclic amino groups exhibited better antibacterial activity than those bearing acyclic ones in most cases. This study provides useful guidelines for further antibacterial reagent design and investigations.
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http://dx.doi.org/10.1021/acsami.1c02474DOI Listing
September 2021

Supercritical CO Synthesis of Freestanding Se S Foamy Cathodes for High-Performance Li-Se S Battery.

Front Chem 2021 28;9:738977. Epub 2021 Jul 28.

College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou, China.

Selenium-sulfur solid solutions (Se S ) are considered to be a new class of promising cathodic materials for high-performance rechargeable lithium batteries owing to their superior electric conductivity than S and higher theoretical specific capacity than Se. In this work, high-performance Li-Se S batteries employed freestanding cathodes by encapsulating Se S in a N-doped carbon framework with three-dimensional (3D) interconnected porous structure ([email protected]) are proposed. Se S is uniformly dispersed in 3D porous carbon matrix with the assistance of supercritical CO (SC-CO) technique. Impressively, [email protected] host not only provides spatial confinement for Se S and efficient physical/chemical adsorption of intermediates, but also offers a highly conductive framework to facilitate ion/electron transport. More importantly, the Se/S ratio of Se S plays an important role on the electrochemical performance of Li- Se S batteries. Benefiting from the rationally designed structure and chemical composition, [email protected]@SeS cathode exhibits excellent cyclic stability (632 mA h g-1 at 200 cycle at 0.2 A g) and superior rate capability (415 mA h g at 2.0 A g) in carbonate-based electrolyte. This novel [email protected]@SeS cathode not only introduces a new strategy to design high-performance cathodes, but also provides a new approach to fabricate freestanding cathodes towards practical applications of high-energy-density rechargeable batteries.
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http://dx.doi.org/10.3389/fchem.2021.738977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355597PMC
July 2021

Bactericidal efficiency and photochemical mechanisms of micro/nano bubble-enhanced visible light photocatalytic water disinfection.

Water Res 2021 Sep 8;203:117531. Epub 2021 Aug 8.

School of Environment, Northeast Normal University, 2555 Jingyue Street, Changchun 130117, China.

Microbial contamination of water in the form of highly-resistant bacterial spores can cause a long-term risk of waterborne disease. Advanced photocatalysis has become an effective approach to inactivate bacterial spores due to its potential for efficient solar energy conversion alongside reduced formation of disinfection by-products. However, the overall efficiency of the process still requires significant improvements. Here, we proposed and evaluated a novel visible light photocatalytic water disinfection technology by its close coupling with micro/nano bubbles (MNBs). The inactivation rate constant of Bacillus subtilis spores reached 1.28 h, which was 5.6 times higher than that observed for treatment without MNBs. The superior performance for the progressive destruction of spores' cells during the treatment was confirmed by transmission electron microscopy (TEM) and excitation-emission matrix (EEM) spectra determination. Experiments using scavengers of reactive oxygen species (ROSs) revealed that HO and •OH were the primary active species responsible for the inactivation of spores. The effective supply of oxygen from air MNBs helped accelerate the hole oxidation of HO on the photocatalyst (i.e. Ag/TiO). In addition, the interfacial photoelectric effect from the MNBs was also confirmed to contribute to the spore inactivation. Specifically, MNBs induced strong light scattering, consequently increasing the optical path length in the photocatalysis medium by 54.8% at 700nm and enhancing light adsorption of the photocatalyst. The non-uniformities in dielectricity led to a high-degree of heterogeneity of the electric field, which triggered the formation of a region of enhanced light intensity which ultimately promoted the photocatalytic reaction. Overall, this study provided new insights on the mechanisms of photocatalysis coupled with MNB technology for advanced water treatment.
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http://dx.doi.org/10.1016/j.watres.2021.117531DOI Listing
September 2021

Progress in soybean functional genomics over the past decade.

Plant Biotechnol J 2021 Aug 13. Epub 2021 Aug 13.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.

Soybean is one of the most important oilseed and fodder crops. Benefiting from the efforts of soybean breeders and the development of breeding technology, large number of germplasm has been generated over the last 100 years. Nevertheless, soybean breeding needs to be accelerated to meet the needs of a growing world population, to promote sustainable agriculture and to address future environmental changes. The acceleration is highly reliant on the discoveries in gene functional studies. The release of the reference soybean genome in 2010 has significantly facilitated the advance in soybean functional genomics. Here, we review the research progress in soybean omics (genomics, transcriptomics, epigenomics and proteomics), germplasm development (germplasm resources and databases), gene discovery (genes that are responsible for important soybean traits including yield, flowering and maturity, seed quality, stress resistance, nodulation and domestication) and transformation technology during the past decade. At the end, we also briefly discuss current challenges and future directions.
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http://dx.doi.org/10.1111/pbi.13682DOI Listing
August 2021

Spatial and Temporal Distribution of Bacterioplankton Molecular Ecological Networks in the Yuan River under Different Human Activity Intensity.

Microorganisms 2021 Jul 19;9(7). Epub 2021 Jul 19.

School of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China.

Bacterioplankton communities play a crucial role in freshwater ecosystem functioning, but it is unknown how co-occurrence networks within these communities respond to human activity disturbances. This represents an important knowledge gap because changes in microbial networks could have implications for their functionality and vulnerability to future disturbances. Here, we compare the spatiotemporal and biogeographical patterns of bacterioplankton molecular ecological networks using high-throughput sequencing of Illumina HiSeq and multivariate statistical analyses from a subtropical river during wet and dry seasons. Results demonstrated that the lower reaches (high human activity intensity) network had less of an average degree (10.568/18.363), especially during the dry season, when compared with the upper reaches (low human activity intensity) network (10.685/37.552) during the wet and dry seasons, respectively. The latter formed more complexity networks with more modularity (0.622/0.556) than the lower reaches (high human activity intensity) network (0.505/0.41) during the wet and dry seasons, respectively. Bacterioplankton molecular ecological network under high human activity intensity became significantly less robust, which is mainly caused by altering of the environmental conditions and keystone species. Human activity altered the composition of modules but preserved their ecological roles in the network and environmental factors (dissolved organic carbon, temperature, arsenic, oxidation-reduction potential and Chao1 index) were the best parameters for explaining the variations in bacterioplankton molecular ecological network structure and modules. , and were the keystone phylum in shaping the structure and niche differentiations in the network. In addition, the lower reaches (high human activity intensity) reduce the bacterioplankton diversity and ecological niche differentiation, which deterministic processes become more important with increased farmland and constructed land area (especially farmland) with only 35% and 40% of the community variation explained by the neutral community model during the wet season and dry season, respectively. Keystone species in high human activity intensity stress habitats yield intense functional potentials and Bacterioplankton communities harbor keystone taxa in different human activity intensity stress habitats, which may exert their influence on microbiome network composition regardless of abundance. Therefore, human activity plays a crucial role in shaping the structure and function of bacterioplankton molecular ecological networks in subtropical rivers and understanding the mechanisms of this process can provide important information about human-water interaction processes, sustainable uses of freshwater as well as watershed management and conservation.
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http://dx.doi.org/10.3390/microorganisms9071532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306320PMC
July 2021
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