Publications by authors named "Xia Qi"

143 Publications

Does the medical insurance system play a real role in reducing catastrophic economic burden in elderly patients with cardiovascular disease in China? Implication for accurately targeting vulnerable characteristics.

Global Health 2021 Mar 29;17(1):36. Epub 2021 Mar 29.

Research Center of Public Policy and Management, School of Health Management, Harbin Medical University, Harbin, 150086, Heilongjiang, China.

Background: The vulnerability of cardiovascular disease (CVD) patients' health abilities, combined with the severity of the disease and the overlapping risk factors, leads such people to bear the economic burden of the disease due to the medical services. We estimated the economic burden of CVD and identified the weak link in the design of the medical insurance.

Methods: Data from 5610 middle-aged and elderly with CVD were drawn from the 2015 wave of "China Health and Retirement Longitudinal Study" (CHARLS). The recommended method of the "World Health Organization" (WHO) was adopted to calculate "catastrophic health expenditure" (CHE), "impoverishment by medical expenses" (IME), and applied the treatment-effect model to analyze the determinants of CHE.

Results: The incidence of CHE was 19.9% for the elderly families with CVD members, which was 3.6% higher than for uninsured families (16.3%). Families with CVD combined with > 3 other chronic diseases (38.88%) were the riskiest factor for the high CHE in the new rural cooperative medical system (NCMS). Moreover, families with members > 75 years old (33.33%), having two chronic disease (30.74%), and families having disabled members (33.33%), hospitalization members (32.41%) were identified as the high risky determinants for the high CHE in NCMS.

Conclusions: Elderly with physical vulnerabilities were more prone to CHE. The medical insurance only reduced barriers to accessing health resources for elderly with CVD; however it lacked the policy inclination for high-utilization populations, and had poorly accurate identification of the vulnerable characteristics of CVD, which in turn affects the economic protection ability of the medical insurance. The dispersion between the multiple medical security schemes leads to the existence of blind spots in the economic risk protection of individuals and families.
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http://dx.doi.org/10.1186/s12992-021-00683-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006647PMC
March 2021

A proteomic approach towards understanding the pathogenesis of Mooren's ulcer.

Exp Eye Res 2021 Apr 27;205:108509. Epub 2021 Feb 27.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao, China; Eye Hospital of Shandong First Medical University, Jinan, Shandong Province, China. Electronic address:

Mooren's ulcer (MU) is a refractory autoimmune corneal ulcer with a high recurrence rate. So far, its molecular profiles and pathomechanisms remain largely unknown. Therefore, we aim to characterize the protein profiles of MU specimens by data-independent-acquisition (DIA) mass spectrometry (MS), and to define the functions of differentially-expressed proteins (DEPs). Through LC-MS/MS, 550 DEPs were identified between MU biopsies and age-matched controls (Ctrl). KEGG analysis revealed that the significantly enriched pathways of the up-regulated proteins mainly covered lysosomes, antigen processing and presentation, and phagosomes. We subsequently validated the expressions of the selected candidates using parallel-reaction-monitoring (PRM)-based MS and immunohistochemistry (IHC), including cathepsins, TIMP3, MMP-10, MYOC, PIGR, CD74, CAT, SOD2, and SOD3. Moreover, immunoglobulin (Ig) components and B lymphocytes associated proteins MZB1, HSPA5, and LAP3 in MU were significantly increased and validated by PRM-based MS and IHC. The remarkable enrichment of neutrophil extracellular traps (NETs) components in MU samples was also identified and determined. The up-regulated Ig components and NETs components suggested that B lymphocytes and neutrophils participated in the immunopathology of MU. Importantly, we also identified and validated much more expression of peptidyl arginine deiminase 4 (PADI4) in MU samples. The double-immunofluorescence staining showed the co-localization of citrulline residues with MPO, NE, and IgG in MU samples. These results indicated the presences of PADI4-mediated citrullination modification and anti-citrullinated protein antibodies (ACPAs) in MU samples. Our findings, for the first time, provide a global proteomic signature of MU, which may open a new avenue towards disease pathology and therapeutics.
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http://dx.doi.org/10.1016/j.exer.2021.108509DOI Listing
April 2021

Therapeutic effect of secretome from TNF-α stimulated mesenchymal stem cells in an experimental model of corneal limbal stem cell deficiency.

Int J Ophthalmol 2021 18;14(2):179-185. Epub 2021 Feb 18.

Department of Gynecology and Obstetrics, Qingdao Municipal Hospital, Qingdao University, Qingdao 266071, Shandong Province, China.

Aim: To explore the secretome efficacy in tumor necrosis factor (TNF)-α stimulated mouse mesenchymal stem cells (MSCs) in a murine model of corneal limbal alkali injury.

Methods: Corneal limbal stem cell deficiency (LSCD) was created in the eyes of male C57 mice. Concentrated conditioned medium from TNF-α stimulated MSCs (MSC-CMT) was applied topically for 4wk, with basal medium and conditioned medium from MSCs as controls. Corneal opacification, corneal inflammatory response, and corneal neovascularization (NV) were evaluated. Corneal epithelial cell apoptosis, corneal conjunctivation, and inflammatory cell infiltration were assessed with TUNEL staining, CK3 and Muc-5AC immunostaining, and CD11b immunofluorescence staining, respectively. The effect of TSG-6 was further evaluated by knockdown with short hairpin RNA (shRNA).

Results: Compared to the controls, topical administration of MSC-CMT significantly ameliorated the clinical symptoms of alkali-induced LSCD, with restrained corneal NV, reduced corneal epithelial cell apoptosis, and inhibition of corneal conjunctivation. In addition, MSC-CMT treatment significantly reduced CD11b inflammatory cell infiltration, and inhibited the expression of pro-inflammatory cytokines (IL-1β, TNF-α and IL-6). Furthermore, the promotion of corneal epithelial reconstruction by MSC-CMT was largely abolished by TSG-6 knockdown.

Conclusion: Our study provides evidence that MSC-CMT enhances the alleviation of corneal alkali injuries, partially through TSG-6-mediated anti-inflammatory protective mechanisms. MSC-CMT may serve as a potential strategy for treating corneal disorders.
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http://dx.doi.org/10.18240/ijo.2021.02.01DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840357PMC
February 2021

MiR-9-5p promotes cell proliferation and migration of hepatocellular carcinoma by targeting CPEB3.

Biomark Med 2021 Feb 26;15(2):97-108. Epub 2021 Jan 26.

Division of Hepatobiliary & Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.

This study aimed to observe the effect of miR-9-5p and CPEB3 on hepatocellular carcinoma (HCC) cells, and investigate the underlying targeting regulatory mechanism. Various experiments like CCK-8, colony formation assay, wound healing assay and Transwell were performed for cancer cell activities detection, including cell proliferation, growth activity, migration and invasion. MiR-9-5p was found to be highly expressed in HCC cells, while CPEB3 was poorly expressed (p < 0.05). The overexpression of miR-9-5p and the silencing of CPEB3 both could significantly promote cell proliferation, migration and invasion (p < 0.05). In addition, miR-9-5p could target to downregulate CPEB3 expression, thus accelerating cell proliferation, migration, invasion and epithelial-mesenchymal transition process in HCC. MiR-9-5p can target CPEB3, thereby promoting cell proliferation, migration and invasion in HCC. The axis of miR-9-5p/CPEB3 is expected to become a potential therapeutic target beneficial for HCC patients.
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http://dx.doi.org/10.2217/bmm-2020-0322DOI Listing
February 2021

Synthesis of layered double hydroxides with nitrate and its adsorption properties of phosphate.

Water Sci Technol 2021 Jan;83(1):100-110

School of Chemical Engineering, Sichuan University, Chengdu 610065, China E-mail: Engineering Research Center for Comprehensive Utilization and Cleaning Process of Phosphate Resource, Ministry of Education, Chengdu 610065, China.

In the present study, different ratios of layered double hydroxides (LDHs) were synthesized via co-precipitation method. The synthesized LDHs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), nitrogen adsorption-desorption analysis, point of zero charges (pH), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Phosphate adsorption performances were estimated by batch adsorption experiments; desorption hysteresis and adsorption mechanism were also investigated. The XRD, SEM and TEM results confirmed the multilayer structure of the synthesized LDHs. The pseudo-second-order kinetic model and the Freundlich model describe the adsorption behavior of LDHs best. The maximum adsorption capacity is 185.86 mg-KHPO/g for MgAl-NO LDH. When the dosage of LDHs was greater than 2 g/L, the phosphorus content in the solution decreased from 30 mg-P/L to 0.077 mg-P/L after adsorption by MgAl-NO LDH. All the results reveal that MgAl-NO LDH is a potential adsorbent for removing phosphate from aqueous solution.
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http://dx.doi.org/10.2166/wst.2020.567DOI Listing
January 2021

Clinical efficacy of Methylprednisolone and the combined use of Lopinavir/Ritonavir with Arbidol in treatment of Coronavirus Disease 2019.

J Med Virol 2021 Jan 15. Epub 2021 Jan 15.

Department of Infectious Disease, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, Zhejiang, China.

Objective: This study aims to comparatively analyze the therapeutic efficacy upon multiple medication plans over Lopinavir/Ritonavir (LPV/r), Arbidol (ARB) and Methylprednisolone on patients with Coronavirus Disease 2019 (COVID-19).

Methods: Totally 75 COVID-19 patients admitted to The First Affiliated Hospital, Zhejiang University School of Medicine from January 22, 2020 to February 29, 2020 were recruited and grouped based on whether or not LPV/r and ARB were jointly used and whether or not Methylprednisolone was used. Indexes including body temperature, time for nucleic acid negative conversion, hospital stays and laboratory indexes were examined and compared.

Results: For all patients, there were no significant differences in the change of body temperature, the time for negative conversion and hospital stays whether LPV/r and ARB were jointly used or not. While for severe and critically severe patients, Methylprednisolone noticeably reduced the time for negative conversion. Meanwhile, the clinical efficacy was superior on patients receiving Methylprednisolone within 3 days upon admission, and the duration of hospital stays was much shorter when Methylprednisolone was given at a total dose of 0-400 mg than a higher dose of >400 mg if all patients received a similar dose per day. Nonetheless, no significant changes across hepatic, renal and myocardial function indexes were observed.

Conclusion: LPV/r combined with ARB produced no noticeably better effect on COVID-19 patients relative to the single agent treatment. Additionally, Methylprednisolone was efficient in severe and critically severe cases, and superior efficacy could be realized upon its early, appropriate and short-term application. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jmv.26798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8013375PMC
January 2021

The retrospective study of perioperative application of dexamethasone and furosemide for postoperative anti-inflammation in patients undergoing percutaneous nephrolithotomy.

Int Urol Nephrol 2021 Apr 7;53(4):669-677. Epub 2021 Jan 7.

Department of Urology, Cheeloo College of Medicine, Shandong Provincial Hospital, Shandong University, No. 324 Jingwu Road, Huaiyin District, Jinan, 250021, Shandong, China.

Objectives: To investigate whether the perioperatively combined application of dexamethasone and furosemide could alleviate the inflammation in patients undergoing percutaneous nephrolithotomy (PCNL).

Patients And Methods: 147 patients undergoing PCNL between November 2018 and October 2019 were enrolled in the study. 77 patients accepted a single dose of dexamethasone and furosemide administration (EXP group, n = 77), and 70 patients did not (CON group, n = 70). Demographic and perioperative data, inflammatory markers including interleukin-6 (IL-6) and procalcitonin (PCT), and clinical outcomes were compared between the two groups.

Results: Compared with the CON group, the incidence rate of urosepsis of the EXP group were significantly lower (11.69% vs. 24.29%, p = 0.046). 3 patients developed severe urosepsis in the EXP group, while 5 patients developed severe urosepsis in the CON group. Compared with those in the CON group, the patients with postoperative urosepsis in the EXP group showed lower serum levels of IL-6 at postoperative hour two (p = 0.045) and at postoperative day one (p = 0.031) and lower serum levels of PCT at postoperative day one (p = 0.015). There was a better clinical outcome of a shorter postoperative hospital stay (p = 0.015) in patients with postoperative urosepsis in the EXP group than in those in the CON group.

Conclusion: The perioperatively combined application of dexamethasone and furosemide was beneficial for alleviating postoperative inflammatory reaction and caused a better clinical outcome of a shorter postoperative hospital stay.
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http://dx.doi.org/10.1007/s11255-020-02718-1DOI Listing
April 2021

Identification and Analysis of the Blood lncRNA Signature for Liver Cirrhosis and Hepatocellular Carcinoma.

Front Genet 2020 7;11:595699. Epub 2020 Dec 7.

Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou, China.

As one of the most common malignant tumors, hepatocellular carcinoma (HCC) is the fifth major cause of cancer-associated mortality worldwide. In 90% of cases, HCC develops in the context of liver cirrhosis and chronic hepatitis B virus (HBV) infection is an important etiology for cirrhosis and HCC, accounting for 53% of all HCC cases. To understand the underlying mechanisms of the dynamic chain reactions from normal to HBV infection, from HBV infection to liver cirrhosis, from liver cirrhosis to HCC, we analyzed the blood lncRNA expression profiles from 38 healthy control samples, 45 chronic hepatitis B patients, 46 liver cirrhosis patients, and 46 HCC patients. Advanced machine-learning methods including Monte Carlo feature selection, incremental feature selection (IFS), and support vector machine (SVM) were applied to discover the signature associated with HCC progression and construct the prediction model. One hundred seventy-one key HCC progression-associated lncRNAs were identified and their overall accuracy was 0.823 as evaluated with leave-one-out cross validation (LOOCV). The accuracies of the lncRNA signature for healthy control, chronic hepatitis B, liver cirrhosis, and HCC were 0.895, 0.711, 0.870, and 0.826, respectively. The 171-lncRNA signature is not only useful for early detection and intervention of HCC, but also helpful for understanding the multistage tumorigenic processes of HCC.
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http://dx.doi.org/10.3389/fgene.2020.595699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750531PMC
December 2020

High pressure laminates reinforced with electrospun cellulose acetate nanofibers.

Carbohydr Polym 2021 Feb 1;254:117461. Epub 2020 Dec 1.

College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, 210037, China.

In the work, the non-woven cellulose acetate (CA) nanofiber mats were prepared via electrospinning, and CA nanofiber were incorporated into the core layer of the high-pressure laminates (HPLs). When the concentration of CA was 16 wt%, SEM images demonstrated that the morphology of the CA nanofiber mat was the best, with an average diameter of 654±246 nm. When CA nanofiber mats were incorporated into the core layer of HPLs, the mechanical properties of the resulted HPLs composites were significantly improved. Specifically, the tensile strength and elongation at break of the nanofiber mats reinforced HPLs composites increased remarkably to 40.8 ±1.1 MPa and 27.9 ± 0.9 %, respectively, which were nearly 6 times and 4.4 times higher than those of the pure HPLs. Furthermore, the incorporation of the CA nanofiber mats also significantly improved the flame retardancy of the HPLs, which was revealed from the thermogravimetric analysis (TGA) results.
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http://dx.doi.org/10.1016/j.carbpol.2020.117461DOI Listing
February 2021

Evaluation of Cannabinoids on the Odonto/Osteogenesis in Human Dental Pulp Cells In Vitro.

J Endod 2021 Mar 20;47(3):444-450. Epub 2021 Jan 20.

Graduate Periodontics, School of Dentistry, University of Detroit Mercy, Detroit, Michigan. Electronic address:

Introduction: Cannabinoids possess anti-inflammatory, analgesic, and osteogenic effects in different cell types and tissues. The null hypothesis is delta-9-tetrahydrocannabinol (THC) might induce dental tissue repair and regeneration. The aim of this study was to investigate the effect of THC on human dental pulp cell (HDPC) viability and biomineralization as well as the molecular mechanism of THC-induced odonto/osteogenic differentiation of HDPCs.

Methods: The toxicity of THC on HDPCs was determined by 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide assay. The odonto/osteogenic differentiation marker genes of HDPCs were assessed by real-time polymerase chain reaction with or without THC treatment. HDPC biomineralization was examined by collagen synthesis and calcium nodule deposition. The molecular mechanism of THC on HDPCs was investigated by examining the mitogen-activated protein kinase (MAPK) signaling pathway via blocking cannabinoid receptor type 1 or 2 receptors.

Results: We found that THC had no inhibition of HDPC vitality in the testing concentration (0-100 μmol/L). THC showed biphasic effects on HDPC proliferation. At a low dose (<5 μmol/L), THC considerably increased HDPC cell division. HDPC proliferation reduced with higher THC concentrations (>5 μmol/L). The expression of odonto/osteogenic marker genes were up-regulated in the presence of cannabinoids. These were confirmed by increased collagen synthesis and mineralized calcium nodule formation in the cannabinoid group. The effect of THC-induced odonto/osteogenesis occurred via MAPK signaling.

Conclusions: THC was biocompatible to HDPCs by promoting their mitogenic division in a biphasic pattern depending on the concentration. THC induced HDPC odonto/osteogenic differentiation through the activation of MAPK mediated by CB1 and CB2 receptors. Cannabinoids may play an important role in the HDPC regeneration process and potentially be used as a pulp-capping agent.
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http://dx.doi.org/10.1016/j.joen.2020.12.005DOI Listing
March 2021

Catalytic Deoxygenation of Xylitol to Renewable Chemicals: Advances on Catalyst Design and Mechanistic Studies.

Chem Rec 2021 Jan 12;21(1):133-148. Epub 2020 Nov 12.

State Key Laboratory of Heavy Oil Processing, College of, Chemical Engineering, China University of Petroleum, No. 66 Changjiang West Road, Qingdao, Shandong Province, 266580, China.

Xylitol is commonly known as one of the top platform intermediates for biomass conversion. Catalytic deoxygenation of xylitol provides an atomic and energetic efficient way to produce a variety of renewable chemicals including ethylene glycol, 1,2-propanediol, lactic acid and 1,4-anhydroxylitol. Despite a few initial attempts in converting xylitol into those products, improving catalyst selectivity towards C-O and C-C cleavage reactions remains a grand challenge in this area. To our best knowledge, there is lack of comprehensive review to summarize the most recent advances on catalyst design and mechanisms in deoxygenation of xylitol, offering important perspective into future development of xylitol transformation technologies. Therefore, in this mini-review, we have critically discussed the conversion routes involved in xylitol deoxygenation over solid catalyst materials, the nanostructures of supported metal catalysts for C-H, C-C and C-O bond cleavage reactions, and mechanistic investigation for xylitol conversion. The outcome of this work provides new insights into rational design of effective deoxygenation catalyst materials for upgrading of xylitol and future process development in converting hemicellulosic biomass.
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http://dx.doi.org/10.1002/tcr.202000101DOI Listing
January 2021

Application value of artificial liver support system in the treatment of cytokine storm in patients with COVID-19.

Int Immunopharmacol 2021 Jan 31;90:107120. Epub 2020 Oct 31.

Department of Infectious Disease, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, Zhejiang, China; Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang University, Zhejiang University, Hangzhou, Zhejiang 310003, China. Electronic address:

Objective: To explore the application value of artificial liver support system in the clinical treatment of coronavirus disease 2019 (COVID-19) patients with cytokine storm.

Methods: Six cases of severe or critically severe COVID-19 patients treated in The First Affiliated Hospital, College of Medicine, Zhejiang University from January 22 to February 4, 2020 were recruited, and all of them received artificial liver support treatment. Statistical analysis was carried out on the change of cytokines (TNF-α, IL-10, IL-6, IFN-γ, IL-2, IL-4), inflammation-related indicators (white blood cell, neutrophil, lymphocyte, C-reactive protein and procalcitonin), immune-related indicators (B lymphocyte percentage, natural killer cell percentage, CD3CD4CD8 T cell percentage), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the 6 patients before and after treatment, and the proportions of patients with abnormal indicators were analyzed as well. In addition, computed tomography (CT) was used to observe the absorption of pulmonary lesions before and after the artificial liver support treatment.

Results: The levels of cytokines (IL-6 and IL-10) were effectively reduced in the 6 patients after treatment with the artificial liver support system. Meanwhile, the proportions of patients with abnormal TNF-α, IL-10, IL-6 and IFN-γ were all decreased (p < 0.05). The levels of inflammation-related indicators including white blood cell, C-reactive protein and procalcitonin, and the proportions of patients with these abnormal indicators were both significantly reduced (p < 0.05). The level of neutrophil was not effectively reduced before and after the treatment, but the proportion was significantly reduced (p < 0.05). However, the abnormality of lymphocyte in the patients was not improved. There was no significant difference in immune-related indicators, AST and ALT before and after the treatment (p > 0.05). CT imaging showed that the artificial liver support treatment contributed to absorption of pulmonary lesions.

Conclusions: The artificial liver support system had a great clinical effect in the treatment of cytokine storm and inflammation in COVID-19 patients, and it could promote the absorption of infected lesions.
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http://dx.doi.org/10.1016/j.intimp.2020.107120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7604162PMC
January 2021

Hyperglycemia-reduced NAD biosynthesis impairs corneal epithelial wound healing in diabetic mice.

Metabolism 2021 01 11;114:154402. Epub 2020 Oct 11.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao, China. Electronic address:

Objective: Nicotinamide adenine dinucleotide (NAD) is an essential molecule participating in multiple physiological and pathophysiological processes. In diabetic cornea, the NAD-consuming enzyme SIRT1 was down-regulated and contributed to the delayed wound healing. However, the impact of hyperglycemia on corneal NAD biosynthesis remained elusive. This study was to investigate the relationship of NAD biosynthesis and the delayed corneal wound healing in diabetic mice.

Methods: Type 1 diabetes mellitus (DM) mice were induced by streptozotocin and corneal epithelial wound healing models were constructed by epithelial scraping. The NAD contents of corneal epithelium were measured using the NAD/NADH quantification kit. Expression of key enzymes involved in the NAD biosynthesis in type 1 DM mice and type 2 DM patients were analyzed. The nicotinamide phosphoribosyltransferase (NAMPT)-specific siRNA and the selective inhibitor FK866 were used to achieve the blockade of NAMPT, whereas exogenous NAD and its precursors were replenished to the corneal epithelial cells and DM mice.

Results: Hyperglycemia attenuated NAD content and NAMPT expression in the corneal epithelium of both type 1 DM mice and type 2 DM patients. Local knockdown of NAMPT by siRNA or FK866 consistently recapitulated the delayed corneal epithelial wound healing in normal mice. Moreover, NAD replenishment recovered the impaired proliferation and migration capacity by either FK866 or high glucose treatment in cultured corneal epithelial cells. Furthermore, in DM mice, NAD and its precursors nicotinamide mononucleotide and nicotinamide riboside also facilitated corneal epithelial and nerve regeneration, accompanied with the recovered expression of SIRT1 and phosphorylated EGFR, AKT, and ERK1/2 in epithelium and corneal sensitivity.

Conclusion: Hyperglycemia-reduced NAD biosynthesis and contributed to the impaired epithelial wound healing in DM mice. The replenishment of NAD and its precursors facilitated diabetic corneal wound healing and nerve regeneration, which may provide a novel therapeutic strategy for the treatment of diabetic corneal complications.
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http://dx.doi.org/10.1016/j.metabol.2020.154402DOI Listing
January 2021

The medical insurance system's weakness to provide economic protection for vulnerable citizens in China: A five-year longitudinal study.

Arch Gerontol Geriatr 2021 Jan - Feb;92:104227. Epub 2020 Aug 14.

Harbin Medical University, Policy and Management Research Center, School of Health Management, Department of Social Medicine; School of Public Health, No. 157 Baojian Road, Nangang District, Harbin, Heilongjiang, 150086, China. Electronic address:

Objectives: Little is known about the magnitude of catastrophic health expenditure (CHE) attributable to critical disease, especially in the middle-aged and elderly population. This research aimed to exploring the key aspects of how the health insurance fails to protect the middle-aged and elderly against CHE in the past five years. And propose corresponding measures to improve.

Methods: Data were obtained from the 2011 to 2015 China Health and Retirement Longitudinal Study. The method was adapted from WHO to calculate the catastrophic health expenditure (CHE) and impoverishment by medical expense (IME), and use Generalized Linear Mixed Models (GLMMs) to comprehensively analyze the risk factors that cause middle-aged and elderly people to fall into CHE.

Results: The incidence of CHE of China's middle-aged and elderly population has been rose in the five years from 2011 (10.5 %) to 2013 (17.5 %) to 2015 (19.7 %). The CHE of richest families was almost 6 times from 2011 to 2015. Urban Employee Medical Insurance Scheme, the incidence of CHE was up 10 percentage from 2011 to 2015. According to the GLMMs, families have inpatient cares as the most important factor to CHE. The incidence of CHE increased by 2.25 times compared with those who did not use inpatient services.

Conclusions: The health system needs to control the irrational growth of health expenses and reduce residents' overuse of health services. Government should take supplementary measures to comprehensively strengthen the advantages of health insurance. Raise residents' awareness of health care, enhance citizens' physical fitness, and avoid unnecessary waste of health resources.
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http://dx.doi.org/10.1016/j.archger.2020.104227DOI Listing
February 2021

Comprehensive analysis of differentially expressed microRNAs and mRNAs involved in diabetic corneal neuropathy.

Life Sci 2020 Nov 19;261:118456. Epub 2020 Sep 19.

Qingdao Eye Hospital of Shandong First Medical University, Qingdao 266071, China; State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao 266071, China. Electronic address:

Aims: Corneal nerve fibers are derived from the ophthalmic division of the trigeminal ganglion (TG). Here, by sequencing of microRNAs (miRNAs) and messenger RNAs (mRNAs) from diabetic and normal TG tissues, we aimed to uncover potential miRNAs, mRNAs, and the network of their interactions involved in the pathogenesis of diabetic corneal neuropathy.

Main Methods: We performed RNA sequencing to systematically screen out differentially expressed miRNAs and mRNAs in TG tissues from diabetic and normal mice. Functional enrichment analyses were performed to illustrate the biological functions of differentially expressed mRNAs (DEmRNAs). Following this, miRNA-mRNA regulatory networks were built by means of bioinformatics methods to suggest regulatory role for miRNAs in the pathogenesis of diabetic corneal neuropathy. Finally, the credibility of the sequencing-based results was validated using qRT-PCR.

Key Findings: Sequencing analyses disclosed that 68 miRNAs and 114 mRNAs were differentially expressed in diabetic TG tissues compared with normal TG samples. The functional analyses showed that DEmRNAs participated in diabetes-related biological processes. After applying an optimized approach to predict miRNA-mRNA pairs, a miRNA-mRNA interacting network was inferred. Subsequently, the expression and correlation of miR-350-5p and Mup20, miR-592-5p and Angptl7 as well as miR-351-5p and Elovl6 were preliminarily validated.

Significance: Our study provides a systematic characterization of miRNA and mRNA expression in the TG during diabetic corneal neuropathy and will contribute to the development of clinical diagnostic and therapeutic strategies for diabetic corneal neuropathy.
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http://dx.doi.org/10.1016/j.lfs.2020.118456DOI Listing
November 2020

Targeting vulnerable groups of health poverty alleviation in rural China- what is the role of the New Rural Cooperative Medical Scheme for the middle age and elderly population?

Int J Equity Health 2020 09 14;19(1):161. Epub 2020 Sep 14.

Policy and Management Research Center, School of Health Management, Department of Social Medicine, School of Public Health, Harbin Medical University, No.157 Baojian Road, Nangang District, Harbin, 150086, Heilongjiang, China.

Background: In light of the health poverty alleviation policy, we explore whether the New Rural Cooperative Medical System (NRCMS) has effectively reduced the economic burden of medical expenses on rural middle-aged and elderly people and other impoverished vulnerable groups. The study aims to provide evidence that can be used to improve the medical insurance system.

Methods: Data were obtained from the 2015 China Health and Retirement Longitudinal Study (CHARLS). The method of calculating the catastrophic health expenditure (CHE) and impoverishment by medical expense (IME) was adopted from the World Health Organization (WHO). The treatment effect model was used to identify the determinants of CHE for rural middle-aged and elderly people.

Results: The incidence of CHE in rural China for middle-aged and elderly people is 21.8%, and the IME is 8.0%. The households that had enrolled in the NRCMS suffered higher CHE (21.9%) and IME (8.0%), than those that had not enrolled (CHE: 20.6% and IME: 7.7%). The NRCMS did not provide sufficient economic protection from CHE for households with three or more chronic diseases, inpatients, or households with members aged over 65 years. Key risk factors for the CHE included education levels, households with inpatients, households with members aged over 65 years, and households with disabilities.

Conclusions: Although the NRCMS has reduced barriers to the usage of household health services by reducing people's out-of-pocket payments, it has not effectively reduced the risk of these households falling into poverty. Our research identifies the characteristics of vulnerable groups that the NRCMS does not provide enough support for, and which puts them at a greater risk of falling into poverty due to health impoverishment.
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http://dx.doi.org/10.1186/s12939-020-01236-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489030PMC
September 2020

Treatment of mandibular grade III furcation involvement using platelet-rich fibrin and allogenic graft with 12-month follow-up - A case report.

J Oral Biol Craniofac Res 2020 Oct-Dec;10(4):542-546. Epub 2020 Aug 18.

University of Detroit Mercy School of Dentistry, Detroit, MI, USA.

Introduction: Furcation involvement (FI) in multi-rooted teeth is challenging for proper oral hygiene, clinical treatment, and leads to poor prognosis. Traditional treatment modalities often result in sacrificing periodontal bone. Multiple regenerative approaches have been attempted to treat furcation defects, but complete regeneration of the periodontal apparatus in grade III furcation has not been reported. Platelet rich fibrin (PRF) shows great potential in enhancing tissue regeneration, angiogenesis, and prevention of infection. This case report introduces a treatment combining allogenic bone grafts with PRF to treat mandibular grade III furcation lesions with a one-year follow-up.

Case Presentation: Two patients presented with grade III FIs of the mandibular first molars, with intrabony defects requiring guided tooth regeneration (GTR). PRF was collected from each patient to serve as biologics, by mixing with allogenic bone graft, and packed into the furcation and intrabony defects. The PRF membranes were also used for space maintenance. The twelve-month postoperative follow-up demonstrated quicker tissue healing, significant pocket reduction, clinical attachment gain, as well as radiographic bone fill in both cases.

Conclusion: Successful periodontal regeneration of grade III furcation defects can be achieved by using PRF in combination with bone allograft.
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http://dx.doi.org/10.1016/j.jobcr.2020.08.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473999PMC
August 2020

A Novel End-To-End Fault Diagnosis Approach for Rolling Bearings by Integrating Wavelet Packet Transform into Convolutional Neural Network Structures.

Sensors (Basel) 2020 Sep 2;20(17). Epub 2020 Sep 2.

School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

Accidental failures of rotating machinery components such as rolling bearings may trigger the sudden breakdown of the whole manufacturing system, thus, fault diagnosis is vital in industry to avoid these massive economical costs and casualties. Since convolutional neural networks (CNN) are poor in extracting reliable features from original signal data, the time-frequency analysis method is usually called for to transform 1D signal into a 2D time-frequency coefficient matrix in which richer information could be exposed more easily. However, realistic fault diagnosis applications face a dilemma in that signal time-frequency analysis and fault classification cannot be implemented together, which means manual signal conversion work is also needed, which reduces the integrity and robustness of the fault diagnosis method. In this paper, a novel network named WPT-CNN is proposed for end-to-end intelligent fault diagnosis of rolling bearings. WPT-CNN creatively uses the standard deep neural network structure to realize the wavelet packet transform (WPT) time-frequency analysis function, which seamlessly integrates fault diagnosis domain knowledge into deep learning algorithms. The overall network architecture can be trained with gradient descent backpropagation algorithms, indicating that the time-frequency analysis module of WPT-CNN is also able to learn the dataset characteristics, adaptively representing signal information in the most suitable way. Two experimental rolling bearing fault datasets were used to validate the proposed method. Testing results showed that WPT-CNN obtained the testing accuracies of 99.73% and 99.89%, respectively, in two datasets, which exhibited a better and more reliable diagnosis performance than any other existing deep learning and machine learning methods.
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http://dx.doi.org/10.3390/s20174965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506762PMC
September 2020

The lncRNA mediates renal cell cancer progression by regulating transcription and EGFR sialylation.

J Cell Sci 2020 08 25;133(16). Epub 2020 Aug 25.

College of Laboratory Medicine, Dalian Medical University, Dalian 116044, Liaoning Province, China

Long noncoding RNAs (lncRNAs) have emerged as important regulators of cancer progression. Abnormal sialylation leads to renal cell carcinoma (RCC) malignancy. However, the mechanism by which the lncRNA maternally expressed gene 3 () mediates RCC progression by regulating transcription and EGFR sialylation is still unrevealed. Here, we found that the expression of was higher in adjacent tissues than in RCC tissues, as well as downregulated in RCC cell lines compared to expression in normal renal cells. The proliferation, migration and invasion of RCC cells transfected with was decreased, whereas knockdown of had the opposite effect. The proliferative and metastatic abilities of RCC cells were concordant with their behavior expression was dysregulated in RCC and was positively correlated with By applying bioinformatics, c-Jun (also known as JUN) was identified as a transcription factor predicted to bind the promoter of , and altered levels resulted in changes to c-Jun expression. Furthermore, ST3Gal1 modulated EGFR sialylation to inhibit EGFR phosphorylation, which affected activation of the phosphoinositide 3-kinase (PI3K)-AKT pathway. Taken together, our findings provide a novel mechanism to elucidate the role of the -ST3Gal1-EGFR axis in RCC progression.
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http://dx.doi.org/10.1242/jcs.244020DOI Listing
August 2020

PTEN Inhibition Facilitates Diabetic Corneal Epithelial Regeneration by Reactivating Akt Signaling Pathway.

Transl Vis Sci Technol 2020 02 7;9(3). Epub 2020 Feb 7.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao, China.

Purpose: To investigate the contribution of phosphatase and tensin homologue (PTEN) on the delayed epithelial regeneration and impaired Akt activation in diabetic mice.

Methods: The expression of PTEN on cornea was compared between normal and diabetic mice. The corneal epithelial and nerve regeneration rate was evaluated in diabetic mice after the treatment with PTEN small interfering RNA (siRNA), PTEN inhibitors, or Akt inhibitor. The reactivation of epithelial regeneration-related signaling, including phosphorylated ()-Akt, -Stat3, Sirt1, and Parkin, were assessed with Western blot and immunofluorescence staining. The effects of PTEN inhibition on cellular proliferation and migration were further evaluated in cultured mouse corneal epithelial cells.

Results: PTEN messenger RNA and protein levels exhibited up-regulation in diabetic cornea. Upon central epithelial debridement, the epithelial regeneration rate was significantly promoted in diabetic mice with the treatment of PTEN inhibition than that of vehicle control ( < 0.05), which accompanied with the recovered levels of -Akt, -Stat3, Sirt1, and Parkin. However, the promotion of diabetic corneal epithelial regeneration rate and Akt reactivation was completed reversed by Akt inhibitor. In vitro, PTEN inhibition promoted their migration, but not the proliferation capacity. In addition, PTEN inhibitor treatment also improved the recovery of corneal nerve fiber density and sensitivity that was impaired in diabetic mice.

Conclusions: Elevated PTEN expression contributes to the impaired corneal epithelial regeneration and Akt activation in diabetic mice, which can be improved with PTEN inhibition.

Translational Relevance: Our study suggests that PTEN inhibition may serve as a new strategy for restoring the impaired corneal epithelial regeneration ability in patients with diabetes.
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http://dx.doi.org/10.1167/tvst.9.3.5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347282PMC
February 2020

Effect of environmental tobacco smoke on COX-2 and SHP-2 expression in a periodontitis rat model.

Oral Dis 2021 Mar 3;27(2):338-347. Epub 2020 Sep 3.

School and Hospital of Stomatology, Hebei Medical University & Hebei Key Laboratory of Stomatology, Shijiazhuang, China.

Objectives: To investigate the effects of environmental tobacco smoke (ETS) on the inflammatory process of periodontitis by evaluating bone loss and the expression of cyclooxygenase-2 (COX-2) and Src homology phosphotyrosine phosphatase 2 (SHP-2).

Materials And Methods: Eighty 6-month-old male SD rats were randomized into four groups (10 rats/group/per time point): (a) normal group, (b) ETS group, (c) ligature-induced periodontitis group, and (d) ligature-induced periodontitis + ETS group. After treatment with ligature and/or ETS for 8 and 12 weeks, the levels of alveolar bone resorption and the expressions of COX-2 and SHP-2 in periodontal tissue were analyzed using histology and immunohistochemistry.

Results: The ligature-induced periodontitis group displayed increased bone resorption and elevated expression of COX-2 and SHP-2 in periodontal tissues compared to the normal and ETS groups at 8 and 12 weeks. Furthermore, bone resorption and COX-2 and SHP-2 levels in the ligature-induced periodontitis + ETS group were significantly increased compared to those in the normal and ligature-induced periodontitis groups at both 8 and 12 weeks.

Conclusion: Environmental tobacco smoke increased alveolar bone loss in periodontitis with enhanced expression of COX-2 and SHP-2 in periodontal tissues. Further investigation is needed to explore the role of COX-2 and SHP-2 in ETS-associated periodontitis.
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http://dx.doi.org/10.1111/odi.13538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818459PMC
March 2021

Effect of ulinastatin on post-operative blood loss and allogeneic transfusion in patients receiving cardiac surgery with cardiopulmonary bypass: a prospective randomized controlled study with 10-year follow-up.

J Cardiothorac Surg 2020 May 14;15(1):98. Epub 2020 May 14.

Department of Anaesthesiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Beilishi Rd., Xicheng District, Beijing, 100037, China.

Background: Major bleeding and allogeneic transfusion leads to negative outcomes in patients receiving cardiac surgery with cardiopulmonary bypass (CPB). Ulinastatin, a urine trypsin inhibitor, relieves systemic inflammation and improves coagulation profiles with however sparse evidence of its effects on blood loss and allogeneic transfusion in this specific population.

Methods: In this prospective randomized controlled trial, 426 consecutive patients receiving open heart surgery with CPB were randomly assigned into three groups to receive ulinastatin (group U, n = 142), tranexamic acid (group T, n = 143) or normal saline (group C, n = 141). The primary outcome was the total volume of post-operative bleeding and the secondary outcome included the volume and exposure of allogeneic transfusion, the incidence of stroke, post-operative myocardial infarction, renal failure, respiratory failure and all-cause mortality. A ten-year follow-up was carried on to evaluate long-term safety.

Results: Compared with placebo, ulinastatin significantly reduced the volume of post-operative blood loss within 24 h (688.39 ± 393.55 ml vs 854.33 ± 434.03 ml MD - 165.95 ml, 95%CI - 262.88 ml to - 69.01 ml, p < 0.001) and the volume of allogeneic erythrocyte transfusion (2.57 ± 3.15 unit vs 3.73 ± 4.21 unit, MD-1.16 unit, 95%CI - 2.06 units to - 0.26 units, p = 0.002). The bleeding and transfusion outcomes were comparable between the ulinastatin group and the tranexamic acid group. In-hospital outcomes and 10-year follow-up showed no statistical difference in mortality and major morbidity among groups.

Conclusions: Ulinastatin reduced post-operative blood loss and allogeneic erythrocyte transfusion in heart surgery with CPB. The mortality and major morbidity was comparable among the groups shown by the 10-year follow-up.

Trial Registration: The trial was retrospectively registered on February 2, 2010.

Trial Registration Number: https://www.clinicaltrials.gov Identifier: NCT01060189.
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http://dx.doi.org/10.1186/s13019-020-01144-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226984PMC
May 2020

LncRNA MEG3 contributes to drug resistance in acute myeloid leukemia by positively regulating ALG9 through sponging miR-155.

Int J Lab Hematol 2020 Aug 2;42(4):464-472. Epub 2020 May 2.

College of Laboratory Medicine, Dalian Medical University, Dalian, China.

Introduction: The development of drug resistance is the main obstacle for successful treatment in acute myeloid leukemia (AML). Noncoding RNAs have been implicated in biological function in AML drug resistance. Aberrant protein glycosylation is associated with AML progression. The aim of the study was to explore the potential regulatory mechanism of lncRNA MEG3/miR-155/ALG9 axis in drug resistance of AML.

Methods: QRT-PCR and Western blot were used for comparison analyses of ALG9, MEG3, and miR-155 levels. CCK-8 and colony formation assays were determined for drug sensitivity and proliferative capability of AML cells. Luciferase reporter assay was used to confirm the targets of miR-155.

Results: The mannosyltransferase ALG9 and MEG3 was downregulated in peripheral blood mononuclear cells (PBMCs) of M5/multidrug resistance (MDR) AML patients and adriamycin (ADR)-resistant AML cell lines, which determined a positive correlation in AML patients. Low expression of ALG9 and MEG3 predicted poor prognosis of AML patients. The altered level of ALG9 was found corresponding to the drug-resistant phenotype and sphere formation of AML cells. MiR-155 was overexpressed in M5/MDR patients and ADR-resistant AML cells, as well as inversely correlated to ALG9 expression. MEG3 was a direct target of miR-155 and could sponge miR-155 in AML cells. MEG3 interacted with miR-155 to regulate ALG9 expression, which reversed the effects of ALG9 regulation on proliferation and drug resistance in AML cells.

Conclusion: MEG3 sponged miR-155 by competing endogenous RNA (ceRNA) mechanism, which further modulated ALG9 expression and AML procession, providing a novel therapeutic target for AML chemoresistance.
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http://dx.doi.org/10.1111/ijlh.13225DOI Listing
August 2020

MANF Promotes Diabetic Corneal Epithelial Wound Healing and Nerve Regeneration by Attenuating Hyperglycemia-Induced Endoplasmic Reticulum Stress.

Diabetes 2020 06 20;69(6):1264-1278. Epub 2020 Apr 20.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Qingdao, Shandong, China

Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a neurotrophic factor widely expressed in mammalian tissues, and it exerts critical protective effects on neurons and other cell types in various disease models, such as those for diabetes. However, to date, the expression and roles of MANF in the cornea, with or without diabetic keratopathy (DK), remain unclear. Here, we demonstrate that MANF is abundantly expressed in normal corneal epithelial cells; however, MANF expression was significantly reduced in both unwounded and wounded corneal epithelium in streptozotocin-induced type 1 diabetic C57BL/6 mice. Recombinant human MANF significantly promoted normal and diabetic corneal epithelial wound healing and nerve regeneration. Furthermore, MANF inhibited hyperglycemia-induced endoplasmic reticulum (ER) stress and ER stress-mediated apoptosis. Attenuation of ER stress with 4-phenylbutyric acid (4-PBA) also ameliorated corneal epithelial closure and nerve regeneration. However, the beneficial effects of MANF and 4-PBA were abolished by an Akt inhibitor and Akt-specific small interfering RNA (siRNA). Finally, we reveal that the subconjunctival injection of MANF-specific siRNA prevents corneal epithelial wound healing and nerve regeneration. Our results provide important evidence that hyperglycemia-suppressed MANF expression may contribute to delayed corneal epithelial wound healing and impaired nerve regeneration by increasing ER stress, and MANF may be a useful therapeutic modality for treating DK.
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http://dx.doi.org/10.2337/db19-0835DOI Listing
June 2020

Does the medical insurance system really achieved the effect of poverty alleviation for the middle-aged and elderly people in China? Characteristics of vulnerable groups and failure links.

BMC Public Health 2020 Apr 3;20(1):435. Epub 2020 Apr 3.

Department of Social Medicine, School of Health Management, Harbin Medical University, Harbin, 150086, Heilongjiang, China.

Background: We examined the physiological, household, and spatial agglomeration characteristics of the health poverty population in China. We identified weak links that affect the implementation of the medical insurance and further improve its effectiveness for health poverty alleviation.

Methods: A national representative sample from the China Health and Retirement Longitudinal Study (CHARLS) was analyzed. The WHO recommended method was adopted to calculate catastrophic health expenditure (CHE) and impoverishment by medical expenses (IME). We created a binary indicator for IME as the outcome variable and applied the treatment-effect model to analyze the determinants of IME.

Results: The incidence of IME was 7.2% of the overall population, compared to 20.3% of the sample households trapped in CHE. The incidence of IME enrolled in insurance schemes was 7.4% higher than that of uninsured families (4.8%). Economic level, living area, family size, age of household head, having hospitalized members, and participating in insurance were statistically significant for the occurrence of IME.

Conclusions: The original poverty-promoting policies has not reached the maximum point of convergence with China's current demand for health. The overlapped health vulnerabilities exacerbated the risk of poverty among the elderly and households with high health needs and utilization. In addition, the medical insurance schemes have proven to be insufficient for protection against economic burden of poor households. So, special health needs, age, and household capacity to pay should be comprehensively considered while strengthening the connection between the disease insurance scheme with supplementary insurance.
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http://dx.doi.org/10.1186/s12889-020-08554-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118817PMC
April 2020

Multi-parameter sensing based on surface plasma resonance with tungsten disulfide sheets coated.

Opt Express 2020 Mar;28(5):6084-6094

A tungsten disulfide (WS) coated surface plasmon resonance (SPR) sensor based on gradient pitch Mach-Zehnder interferometer (GP-MZI) for measuring ethanol vapor concentration is proposed and verified by experiments. Under continuous CO laser heating, a MZI based on GP helix structure is fabricated by twisting single mode fiber (SMF), which can excites multi-order cladding modes. A gold film is deposited on the surface of the GP helix structure by a magnetic sputtering coating machine. WS film is coated on the gold film of the GP helix structure, which increases the evanescent field strength of the twisted structure surface and enhances the interaction between SPR wave and ethanol molecules. Since the absorption of ethanol molecules by WS sheets will cause the change of effective refractive index (RI) of WS film, the intensity of transmission signal can be adjusted accordingly. For multi-order cladding modes, the effective RI and the effective thermo-optic coefficient vary with the modal order, so the RI and temperature sensitivity of different modal orders are also different. So, the ethanol vapor concentration, relative humidity (RH), and temperature can be simultaneously measured by monitoring the intensity of those dips with the resolution of ± 0.030 mg/L, ±0.035%RH, and ± 0.010 ℃, respectively. This sensor structure provides a promising platform for multi-parameter sensing applications.
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http://dx.doi.org/10.1364/OE.385251DOI Listing
March 2020

Author Correction: Combination of chick embryo and nutrient mixture prevent D-galactose-induced cognitive deficits, immune impairment and oxidative stress in aging rat model.

Sci Rep 2020 Mar 13;10(1):4978. Epub 2020 Mar 13.

College of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41598-020-61999-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067856PMC
March 2020

The Role of Cariporide in Protecting Saphenous Vein among Different Aldehyde Dehydrogenase Genotypes.

Cardiology 2020 26;145(7):456-466. Epub 2020 Feb 26.

Department of Cardiovascular Surgery, Zhongshan Hospital, Fudan University, Shanghai, China,

Objective: We aimed to study the effect of cariporide (CP) on protecting the saphenous vein and the role of acetaldehyde dehydrogenase 2 (ALDH2) in coronary artery bypass grafting (CABG).

Background: The saphenous vein is the main graft material used in CABG. Recent studies suggested that CP is effective in protecting against various cardiovascular diseases.

Methods: Segments of a surgically removed saphenous vein were used to examine the vascular response to CP. The ALDH2 genotype and expression of related proteins were assessed by Western blotting and immunohistochemistry.

Results: Among the conditions tested, the University of Wisconsin solution with CP (4°C, 5 min) treatment showed the best protective effect on the saphenous vein. The incidence of major adverse cardiac events was higher in the ALDH2-GA (heterozygous mutant) genotype population after CABG.

Conclusion: CP plays a role in reducing the production of reactive oxygen species and apoptosis by ALDH2-mediated mitochondrial function improvement. The ALDH2 mutant genotype might be one of the risk factors for coronary atherosclerotic heart disease.
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http://dx.doi.org/10.1159/000506071DOI Listing
April 2021

Location of Balanced Chromosome-Translocation Breakpoints by Long-Read Sequencing on the Oxford Nanopore Platform.

Front Genet 2019 14;10:1313. Epub 2020 Jan 14.

Institute of Reproduction and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.

Genomic structural variants, including translocations, inversions, insertions, deletions, and duplications, are challenging to be reliably detected by traditional genomic technologies. In particular, balanced translocations and inversions can neither be identified by microarrays since they do not alter chromosome copy numbers, nor by short-read sequencing because of the unmappability of short reads against repetitive genomic regions. The precise localization of breakpoints is vital for exploring genetic causes in patients with balanced translocations or inversions. Long-read sequencing techniques may detect these structural variants in a more direct, efficient, and accurate manner. Here, we performed whole-genome, long-read sequencing using the Oxford Nanopore GridION sequencer to detect breakpoints in six balanced chromosome translocation carriers and one inversion carrier. The results showed that all the breakpoints were consistent with the karyotype results with only ~10× coverage. Polymerase chain reaction (PCR) and Sanger sequencing confirmed 8 out of 14 breakpoints; however, other breakpoint loci were slightly missed since they were either in highly repetitive regions or pericentromeric regions. Some of the breakpoints interrupted normal gene structure, and in other cases, micro-deletions/insertions were found just next to the breakpoints. We also detected haplotypes around the breakpoint regions. Our results suggest that long-read, whole-genome sequencing is an ideal strategy for precisely localizing translocation breakpoints and providing haplotype information, which is essential for medical genetics and preimplantation genetic testing.
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http://dx.doi.org/10.3389/fgene.2019.01313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972507PMC
January 2020