Publications by authors named "Xia Dou"

19 Publications

  • Page 1 of 1

Practical algorithm to inform clinical decision-making in the topical treatment of atopic dermatitis.

J Dermatol 2021 Aug 7;48(8):1139-1148. Epub 2021 May 7.

Department of Dermatology and Venereology, Peking University First Hospital, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, National Clinical Research Center for Skin and Immune Diseases, Beijing, China.

Atopic dermatitis is a chronic relapsing, inflammatory skin disorder associated with skin barrier dysfunction, the prevalence of which has increased dramatically in developing countries. In this article, we propose a treatment algorithm for patients with mild-to-moderate and severe atopic dermatitis flares in daily clinical practice. An international panel of 15 dermatology and allergy experts from eight countries was formed to develop a practical algorithm for the treatment of patients with atopic dermatitis, with a particular focus on topical therapies. In cases of mild-to-moderate atopic dermatitis involving sensitive skin areas, the topical calcineurin inhibitor pimecrolimus should be applied twice daily at the first signs of atopic dermatitis. For other body locations, patients should apply a topical calcineurin inhibitor, either pimecrolimus or tacrolimus, twice daily at the first signs of atopic dermatitis, such as pruritus, or twice weekly in previously affected skin areas. Emollients should be used regularly. Patients experiencing acute atopic dermatitis flares in sensitive skin areas should apply a topical corticosteroid twice daily or alternate once-daily topical corticosteroid/topical calcineurin inhibitor until symptoms improve. Following improvement, topical corticosteroid therapy should be discontinued and patients switched to a topical calcineurin inhibitor. Maintenance therapy should include the use of pimecrolimus once daily for sensitive areas and tacrolimus for other body locations. This treatment algorithm can help guide clinical decision-making in the treatment of atopic dermatitis.
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http://dx.doi.org/10.1111/1346-8138.15921DOI Listing
August 2021

Impact of genetic variants in IL-2RA and IL-2RB on breast cancer risk in Chinese Han women.

Biochem Genet 2021 Jun 28;59(3):697-713. Epub 2021 Jan 28.

Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, School of Life Sciences, Northwest University, #229 North TaiBai Road, Xi'an, Shaanxi, 710069, China.

The expression of IL-2RA and IL-2RB was correlated with breast cancer (BC) progression. However, there is no literature investigating the association of IL-2RA and IL-2RB polymorphisms with BC predisposition among Chinese Han Women. Seven SNPs in IL-2RA and IL-2RB were genotyped by Agena MassARRAY platform among 553 BC patients and 550 healthy controls. Odds ratios (OR) and 95% confidence interval (CI) adjusted for age were calculated for the effect of IL-2RA and IL-2RB variants on BC susceptibility. IL-2RA rs12722498 was a protective factor for BC occurrence (OR = 0.70, p = 0.019), especially in subjects with age ≤ 52 years (OR = 0.55, p = 0.004). IL-2RA rs12569923 (OR = 9.07, p = 0.033), IL-2RB rs2281089 (OR = 0.67, p = 0.043) and rs9607418 (OR = 0.59, p = 0.012) were related to the incidence of estrogen receptor positive (ER +) BC. IL-2RB rs3218264 (OR = 1.38, p = 0.010) and rs9607418 (OR = 0.56, p = 0.009) were associated with the risk of developing progesterone receptor positive (PR +) BC. Rs2281089 (OR = 1.54, p = 0.012) and rs1573673 (OR = 0.72, p = 0.035) were correlated to Ki-67 level. Moreover, IL-2RB rs2281089 (OR = 0.72, p = 0.022) showed a reduced risk of BC metastasis, and IL-2RA rs12722498 (OR = 0.54, p = 0.030) had a lower frequency in BC patients with tumor size > 2 cm. Our study identified the potential effect of genetic variations in IL-2RA and IL-2RB on BC susceptibility and/or BC clinicopathologic indicators among Chinese Han Women.
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http://dx.doi.org/10.1007/s10528-021-10029-yDOI Listing
June 2021

[Effect of STIL on the Gene Expression Profile of Gastric Cancer Cells].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2019 Dec;41(6):778-786

Teaching and Research Section of Pathological Anatomy,Inner Mongolia Medical University, Hohhot 010010,China.

To explore the molecular mechanism underlying gastric carcinogenesis and progression by using gene expression profiling array together with bioinformatics. Lentivirus short hairpin RNA targeting STIL(ShSTIL)and scrambled sequence RNA(ShCon)were transduced into the gastric cancer cell line SGC-7901.RNA extraction,complementary DNA synthesis,construction of biotin-labelled amplified RNA probes,and hybridization with gene expression profile were consecutively performed.We collected corresponding data and analyzed differentially expressing genes(DEGs),followed by the analysis of gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment,transcription factor regulating network,and protein-protein interacting networks. Compared with ShCon,a total of 417 and 87 genes were respectively down-regulated and up-regulated,respectively,in the ShSTIL group(<0.05,fold change>1 or <-1).GO and KEGG enrichment analysis indicated that genes regulated by STIL were localized in cytoplasm,extracellular exosome,Golgi apparatus and various biomembranes,and were implicated in the ubiquitin-mediated proteolysis,P53 signaling pathway,and pathways regulating pluripotency of stem cells.Evaluation on genes enriched in KEGG pathways,regulation of transcription factors,and protein-protein interacting network demonstrated that IGF1R,STUB1,SKP2,and FOXO1 were localized at the centre of the network and played a key role in the development and progression of gastric cancer. Through the protein-protein interactions,STIL may activate E3 ubiquitin ligase STUB1 or SKP2,promote the proteolysis of FOXO1-a transcription factor,regulate the expression of IGF1R,and thus promote gastric carcinogenesis and progression.
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http://dx.doi.org/10.3881/j.issn.1000-503X.11150DOI Listing
December 2019

Aberrant Expression of Histamine-independent Pruritogenic Mediators in Keratinocytes may be Involved in the Pathogenesis of Prurigo Nodularis.

Acta Derm Venereol 2019 May;99(6):579-586

Department of Dermatology, Peking University First Hospital, Beijing 100034, China.

Prurigo nodularis is a highly pruritic and hyperplastic chronic dermatosis with unknown pathogenesis. Many pruritogenic mediators, including nerve growth factor, interleukin (IL)-31, thymic stromal lymphopoietin, and endothelin-1, are implicated in chronic itch and inflammation. This study investigated the mRNA levels and immunoreactivity of the nerve growth factor, IL-31, thymic stromal lymphopoietin, and endothelin axes in both lesional and perilesional skin in prurigo nodularis by using quantitative real-time PCR and immunohistochemistry studies. The nerve growth factor high-affinity receptor tyrosine kinase receptor A was upregulated while the low affinity receptor p75 neurotrophin receptor was downregulated in prurigo nodularis lesions. Downregulated expression of IL-31/IL-31 receptor A and endothelin-3/endothelin receptor B and upregulation of thymic stromal lymphopoietin receptor were found in prurigo nodularis lesions. Aberrant expression of nerve growth factor, IL-31, thymic stromal lymphopoietin and endothelin axes was found in prurigo nodularis lesions, especially in the epidermis, indicating the importance of keratinocytes in prurigo nodularis pathogenesis.
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http://dx.doi.org/10.2340/00015555-3150DOI Listing
May 2019

Regulatory B cells induced by ultraviolet B through toll-like receptor 4 signalling contribute to the suppression of contact hypersensitivity responses in mice.

Contact Dermatitis 2018 Feb 5;78(2):117-130. Epub 2017 Dec 5.

Department of Dermatology and Venereology, Peking University Shenzhen Hospital, Shenzhen, 518036, China.

Background: Ultraviolet (UV) B irradiation is known to suppress contact hypersensitivity (CHS) responses in mouse models by suppressing immune responses. However, the cellular mechanisms responsible for UVB-induced systemic suppression remain unclear. Regulatory B cells have been reported to play an inhibitory role during CHS. It is presently unknown whether regulatory B cells contribute to the effect of UVB phototherapy.

Objective: To investigate the inductive effect of UVB on regulatory B cells and the underlying mechanisms by using a CHS mouse model.

Methods: CHS was induced with oxazolone, and evaluated by histopathology, flow cytometry, and quantitative real-time polymerase chain reaction.

Result: We found that UVB irradiation induced regulatory B cell expansion and ameliorated CHS. UVB-induced regulatory B cells contribute to systemic immunosuppression by inhibiting the proliferation of T cells. Moreover, we determined that toll-like receptor (TLR) 4, the expression of which was upregulated in B cells after UVB exposure, played an essential role in the induction of regulatory B cells.

Conclusion: Our data identified regulatory B cells as regulators of UVB-induced immunosuppression in CHS, and suggest the importance of the UVB-TLR4 axis in the generation of regulatory B cells.
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http://dx.doi.org/10.1111/cod.12913DOI Listing
February 2018

Delayed allergic hypersensitivity to hyaluronidase during the treatment of granulomatous hyaluronic acid reactions.

J Cosmet Dermatol 2018 Dec 21;17(6):991-995. Epub 2017 Nov 21.

Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, China.

Hyaluronic acid (HA) is now extensively employed for esthetic concerns. In the majority of cases, HA is considered to be safe, but mild-to-severe complications can occur in a few cases. Hyaluronidase is enzymes that degrade HA, also being suggested for the treatment of HA filler-induced complications. However, hyaluronidase has been reported to be a potential cause of allergic responses in some anesthetic and ophthalmic literature. However, there are few reports of allergic reactions to hyaluronidase in cosmetic medicine. We herein report on a 39-year-old Asian woman who developed a delayed allergic hypersensitivity to hyaluronidase in the treatment process of HA-related granulomatous reaction.
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http://dx.doi.org/10.1111/jocd.12461DOI Listing
December 2018

MiR-151a is involved in the pathogenesis of atopic dermatitis by regulating interleukin-12 receptor β2.

Exp Dermatol 2018 04 11;27(4):427-432. Epub 2017 Apr 11.

Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China.

MicroRNAs (miRNAs) have been reported to circulate in the blood in a highly stable and cell-free form. Dysregulated expression of miRNAs has been detected in various pathological conditions including atopic dermatitis. In our study, human blood plasma miRNAs were identified by high-throughput sequencing and compared among patients of atopic dermatitis and healthy controls. We found that miR-151a was differentially expressed in the plasma of atopic dermatitis patients. MiR-151a regulates the expression of IL12RB2 by targeting two loci in the 3' untranslated region of the Il12rb2 gene. Moreover, IL12RB2 was remarkably downregulated in Jurkat cells overexpressing miR-151a. Jurkat cells treated with phytohemagglutinin also showed reduced expression of IFN-γ, interleukin-2 (IL-2) and IL-12. Together, these results suggest that miR-151a is involved in the pathogenesis of atopic dermatitis by regulating IL12RB2.
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http://dx.doi.org/10.1111/exd.13276DOI Listing
April 2018

Development of Unlabeled Probe Based High-Resolution Melting Analysis for Detection of Filaggrin Gene Mutation c.3321delA.

J Clin Lab Anal 2016 Nov 3;30(6):892-896. Epub 2016 Apr 3.

Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, P.R. China.

Background: Filaggrin gene (FLG) plays an important role in skin barrier function, and loss-of-function mutations of FLG have been shown to be a predisposing factor for atopic dermatitis (AD). The c.3321delA mutation is the most common FLG mutation in Chinese population. We aim to develop a rapid, cost-efficiency, and reliable closed-tube method that has not been described for the detection of c.3321delA mutation.

Methods: Recombinant wild-type and mutant plasmids of c.3321delA mutation were constructed, heterozygous mutant plasmids were prepared by mixing the mutant plasmids and wild-type plasmids at 1:1 ratio. High-resolution melting analysis (HRMA) coupled with an unlabeled DNA probe was employed to identify the shift in melting temperature of the probe-template complex, which reflects the presence of c.3321delA mutation.

Results: Unlabeled probe based HRMA was able to distinguish all three genotypes (wild-type, heterozygote, and mutant) of c.3321delA mutation. Then, we applied this method to genotype 1,317 clinical samples. Genotyping results obtained from unlabeled probe HRMA were 100% concordant with the results from direct sequencing.

Conclusion: We developed a fast and high-throughput method to detect the c.3321delA mutation.
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http://dx.doi.org/10.1002/jcla.21953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806714PMC
November 2016

High-level expression and purification of the major house dust mite allergen Der p 2 in Escherichia coli.

Protein Expr Purif 2016 May 23;121:97-102. Epub 2016 Jan 23.

Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, 518036, Guangdong, China; Shenzhen Key Lab for Translational Medicine of Dermatology, Shenzhen Peking University - the Hong Kong University of Science and Technology Medical Center, Shenzhen, 518036, Guangdong, China. Electronic address:

Der p 2, a major allergen derived from the house dust mite Dermatophagoides pteronyssinus, is one of the most clinically relevant allergens worldwide. Recombinant Der p 2 (rDer p 2) is useful in clinical diagnosis and disease-specific immunotherapy. However, previous studies showed that Der p 2 can only be expressed in Escherichia coli (E. coli) cells as inclusion bodies, thus protein refolding is required to obtain functional products. Here we report a new method to produce biologically active Der p 2 protein in E. coli. N-terminal hexahistidine- and trigger factor (TF)-tagged Der p 2 was expressed in soluble form in E. coli and purified using a combination of chromatography processes. This procedure produced milligram-level high purity Der p 2 per liter of bacterial culture. Moreover, far-UV region circular dichroism (CD) analysis and serum specific IgE reactivity test demonstrated that the secondary structure and IgE reactivity properties of rDer p 2 produced in our study were almost identical to those of natural Der p 2 (nDer p 2). In conclusion, the method developed in this work provides a useful tool for the production of immunologically active recombinant Der p 2 for clinical applications.
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http://dx.doi.org/10.1016/j.pep.2016.01.012DOI Listing
May 2016

Impaired Toll-like receptor 2-mediated Th1 and Th17/22 cytokines secretion in human peripheral blood mononuclear cells from patients with atopic dermatitis.

J Transl Med 2015 Dec 18;13:384. Epub 2015 Dec 18.

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China.

Background: Impaired Toll-like receptor 2 (TLR2) function has been associated with the pathogenesis of atopic dermatitis (AD). However, there are only few studies reporting on the TLR2-induced immunological responses of circulating leucocytes of AD patients. We thus investigated the expression and secretion of Th1, Th2 and Th17/22 cytokines triggered by TLR2 ligands in human peripheral blood mononuclear cells (PBMCs) from AD patients. Expression of TLR2, 1, 6 and high-affinity receptor for IgE (FcεRI) were further investigated to evaluate the outcome of immune response in AD.

Methods: Expression of TLR2, 1, 6 and FcεRI in PBMCs from AD patients and healthy individuals were measured by qPCR. Subsequent to stimulation with TLR2 ligands PGN and Pam3CSK4, expression and secretion of Th1, Th2 and Th17/22 cytokines were investigated by qPCR and ELISA.

Results: The levels of TLR2, 1, 6 mRNA were not altered in both groups of subjects while that of FcεRI was increased in AD patients. Subsequent to the activation by TLR2 ligands, PBMCs from AD patients significantly released less IFN-γ, IL-17F and IL-22 than those from healthy controls while no detectable level of release was observed with the other cytokines. In contrast, significantly higher levels of mRNA expression for TNF-α, IL5, IL-17A and IL-22 were observed in TLR2 activated PBMCs of AD patients than those of healthy control.

Conclusions: PBMCs from AD patients are defective in the secretion of Th1 and Th17/22 cytokines in response to TLR2 ligands. The inconsistent increased expression of the mRNA for the corresponding Th1 cytokines and the Th2 cytokines IL-5 suggested that there may be alterations of downstream signaling events in the cytokine release mechanisms of PBMCs that are associated with the development of AD.
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http://dx.doi.org/10.1186/s12967-015-0744-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683963PMC
December 2015

Serum levels of thymus and activation-regulated chemokine can be used in the clinical evaluation of atopic dermatitis.

Int J Dermatol 2015 Jul;54(7):e261-5

Department of Dermatology, Hua Shan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by the predominant infiltration of Th2-type cells in lesional skin. Thymus and activation-regulated chemokine (TARC) is overproduced in AD patients, and its serum levels are significantly higher in individuals with AD than in those with other inflammatory skin diseases.

Objectives: The purpose of this study was to examine whether serum levels of TARC can assess the severity of AD and be used in the clinical evaluation of AD.

Methods: A total of 73 AD patients, 11 patients with generalized atopic eczema (AE), and 30 healthy control subjects were enrolled. SCORAD (SCORing of Atopic Dermatitis) indices were calculated according to skin symptom scores. The Th2 chemokines TARC kit was then used to obtain serum TARC values in each group. Finally, statistical analysis was used to identify any correlations between serum TARC level and SCORAD index in AD and AE patients.

Results: Mean serum TARC values were 159.95 in healthy controls, 146.46 in the mild AD group, 202.71 in the moderate AD group, 1216.61 in the severe AD group and 1554.50 in the severe AE group. The serum TARC level was significantly correlated with SCORAD score in AD patients (P < 0.01). However, there was no significant correlation between SCORAD score and TARC in AE patients (P = 0.610).

Conclusions: The serum TARC level can be used to assess the severity of AD and as a reference index for the fast clinical evaluation of AD.
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http://dx.doi.org/10.1111/ijd.12830DOI Listing
July 2015

[Comparative study of sensitivity of different dental metal materials].

Shanghai Kou Qiang Yi Xue 2014 Apr;23(2):143-8

Department of Stomatology, Huashan Hospital, Fudan University. Shanghai

Purpose: To compare the sensitivity of different dental metal materials, in order to provide references for choosing of dental metal materials.

Methods: Patch test was performed on 92 patients wearing dental metal prosthesis. Pearson Chi-square test, corrected Chi-square test and Fisher exact test were used for statistical analysis with SPSS17.0 software package.

Results: (1)The sensitivity rates of different metal materials were different. The allergy rate of nickel (Ni) was the highest (22.8%), while the allergy rate of aluminum (Al) was 0. (2)More women were allergic to both palladium (Pd) and nickel (Ni) than men with significant difference (P>0.05). (3)Women with ear piercing were more allergic to nickel (Ni), but there was no significant correlation between ear piercing and nickel allergy (P>0.05).(4)There was cross reaction between nickel(Ni)and palladium (Pd), 83.3% of palladium (Pd) allergy patients were allergic to nickel (Ni), while 47.6% of nickel(Ni) allergy patients were allergic to palladium (Pd). (5)Patch test had a delayed reaction.

Conclusions: Dental metal materials have certain allergies, women are more allergic to both palladium (Pd) and nickel (Ni) than men, with significant difference. Patch test may have a delayed reaction. If necessary, observation for 96 h, 7 days or even longer time, are needed to exclude false positivity. Supported by Research Fund of Science and Technology Committee of Shanghai Municipality (10411950900).
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April 2014

Retrospective study of photopatch testing in a Chinese population during a 7-year period.

Dermatitis 2014 Jan-Feb;25(1):22-6

From the Department of Dermatology, Huashan Hospital Fudan University, Shanghai, People's Republic of China.

Background: Photoallergic contact dermatitis (PACD) is of importance in a proportion of photodermatoses and can be evaluated through photopatch testing (PPT).

Objectives: The objectives of this study were to evaluate the results of PPT and investigate the prevalence of PACD reactions to different photoallergens in Chinese patients at the Department of Dermatology of Huashan Hospital Fudan University during a 7-year period.

Methods: A retrospective PPT study was conducted. During the 7 years, 4957 patients attending for investigation of suspected photodermatoses were tested according to the European consensus methodology with up to 14 allergens prepared according to Chinese National Standards. The reactions were scored using the International Contact Dermatitis Research Group visual scoring system.

Results: A total of 3472 PACD reactions in 2454 subjects (49.5%) were recorded. The most common agents were chlorpromazine (44.3%), followed by para-aminobenzoic acid (14.7%), thimerosal (8.9%), and sulfanilamide (6.9%). Allergic contact dermatitis reactions comprised 409 reactions in 399 subjects (8%). Photoinhibition and photoaugmentation of allergic contact dermatitis compromised 3810 reactions in 2412 subjects and 11 reactions in 11 subjects, respectively. Irritant reactions (1928 reactions) were seen in 1140 subjects.

Conclusions: The most predominant photoallergens in our region were chlorpromazine, para-aminobenzoic acid, thimerosal, and sulfanilamide, which likely reflected the particular exposures of this Chinese population.
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http://dx.doi.org/10.1097/DER.0000000000000008DOI Listing
September 2014

Clinical and pathological study of 328 cases of actinic keratosis in eastern chinese patients.

Dermatology 2013 31;227(4):316-20. Epub 2013 Oct 31.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.

Background: Actinic keratosis (AK) is prevalent and has been widely studied in fair-skinned populations. However, this is not the case in eastern countries. AK in Asians has not been so thoroughly investigated.

Objectives: To analyse the clinical and pathological features of a relatively large number of cases of AK diagnosed in older Chinese patients.

Methods: Case histories of 328 patients with pathologically diagnosed AK were analysed retrospectively. Their demographic, clinical, pathological and treatment data were collected for analysis of associated factors.

Results: Lesions were usually distributed on the face, especially the cheeks and temples. The most frequent pathological type was hypertrophic. Only 34% of the cases had been diagnosed correctly as AK before biopsy; many were mistaken for seborrhoeic keratosis.

Conclusions: Most patients were elderly females and there was a higher incidence of lesions on the face, and a lower incidence on the extremities and trunk; this finding contrasts with previous investigations in fair-skinned people.
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http://dx.doi.org/10.1159/000354651DOI Listing
September 2014

Prevalence of contact allergy at a dermatology clinic in China from 1990-2009.

Dermatitis 2011 Nov-Dec;22(6):324-31

Department of Dermatology, Peking University First Hospital, Beijing, China.

Background: The prevalence of contact allergy varies in different regions and populations.

Objective: To describe the frequency of sensitization in patients with dermatitis or eczema referred to Peking University First Hospital and analyze the trends in the prevalence of common allergens from January 1, 1990, to December 31, 2009.

Methods: A total of 1,858 patients were patch tested with the Chinese baseline series of contact allergens. Data were collected from retrospective charts and analyzed.

Results: Positive reactions to one or more allergens were shown in 1,374 patients (74.0%). The most common sensitizers were nickel sulfate (25.7%), fragrance mix I (25.6%), thiuram mix (25.5%), ammoniated mercury (20.5%), and p-phenylenediamine (19.1%). A statistically significant increase of sensitization over the 20-year period was seen for nickel sulfate, fragrance mix, ammoniated mercury, colophony, ethylenediamine, and potassium dichromate. Mercapto mix showed a trend of a statistically significant decrease in sensitizations from 1990 to 2009.

Conclusions: The patterns of contact allergy in patients from Peking University Hospital are different from those of patients in other regions of China, in European countries, and in the United States. Nickel and fragrance mix were the most common allergens, and the sensitization rates of these two allergens had been increasing remarkably during the 20 years from 1990 to 2009.
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http://dx.doi.org/10.2310/6620.2011.11051DOI Listing
October 2012

Contact sensitization in Chinese patients with hand eczema.

Dermatitis 2011 Jul-Aug;22(4):211-5

Department of Dermatology, Peking University First Hospital, Beijing, China.

Background: Contact sensitization is an important cause of hand eczema, a common disease that affects both daily and occupational life.

Objective: To describe contact allergens in Chinese patients with hand eczema and to analyze the association between allergens and occupational exposure.

Methods: Three hundred sixty-six patients with hand eczema underwent patch testing with a modified North American standard series between September 1989 and December 2009.

Results: Positive patch-test reactions were observed in 74% of patients. The most frequent allergens were p-phenylenediamine (22.7%), nickel sulfate (21.9%), fragrance mix (19.9%), ammoniated mercury (19.7%), and carba mix (17.8%). Nickel and fragrance allergy in women, and carba mix and chromate allergy in men, showed gender predilection, and 64.2% of patients developed hand eczema from occupational exposure. Positive reactions to p-phenylenediamine in hairdressers and to chromate in metalworkers and construction workers presented higher frequencies (p < .05).

Conclusions: p-Phenylenediamine, nickel, fragrance, mercury, and rubber chemicals are common allergens in Chinese patients with hand eczema. Gender and occupation may cause different exposures; thus, allergens may vary.
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November 2011

[Expression of nuclear factor kappaB and the effect of topical tacrolimus ointment on lesional atopic dermatitis skin].

Beijing Da Xue Xue Bao Yi Xue Ban 2004 Oct;36(5):487-90

Department of Dermatology, Peking University First Hospital, Beijing 100034, China.

Objective: To investigate the role of nuclear factor kappaB (Rel/NF-kappaB) in pathogenesis of atopic dermatitis(AD) and the effect of topical 0.1%(mass fraction) or 0.03%(mass fraction) tacrolimus ointment on expression of NF-kappaB in lesional AD skin.

Methods: Immunohistochemistry has been employed to study the expression of NF-kappaB in normal skin and lesional AD skin before and after using topical tacrolimus ointment.

Results: The expressions of NF-kappaBp50 and NF-kappaBp65 were scattering or negative in normal keratinocytes. NF-kappaBp50 was overexpressed on nuclear of basal and suprabasal keratinocytes in 9 cases of AD, NF-kappaBp65 was overexpressed in cytoplasm and perinuclear of basal and suprabasal keratinocytes. After using topical tacrolimus ointment for three weeks , nuclear NF-kappaBp50 expressed on basal and suprabasal keratinocytes were lost and NF-kappaBp50 was expressed sparsely on basal keratinocytes cytoplasm or nuclear. NF-kappaBp65 was expressed sparsely on basal and suprabasal keratinocytes cytoplasm.

Conclusion: These data suggest that increased NF-kappaB activity may represent the basis of initiation or maintenance of the skin inflammatory response in atopic dermatitis. Topical tacrolimus may directly or indirectly inhibit NF-kappaB nuclear expression in keratinocytes and inhibit skin innate immuno-inflammatory response in atopic dermatitis that related to NF-kappaB.
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October 2004

Nickel-elicited systemic contact dermatitis.

Contact Dermatitis 2003 Mar;48(3):126-9

Department of Dermatology, Peking University First Hospital, Beijing, China.

20 patients with systemic contact dermatitis due to nickel are described. Of these patients, 15 were female and 5 were male. Their mean age was 24.8 years (16-51 years). All had experienced contact dermatitis in the umbilical area due to continual contact with metal belt-buckles or buttons. Then, with long- or short-term aggravation of such periumbilical dermatitis, commonly in summer, lesions spread to other sites such as the side of the neck, the flexures of the extremities, etc. All patients showed a positive patch test to nickel sulphate (2.5% in petrolatum) and the dimethylglyoxime test demonstrated the presence of free nickel on metal buttons or belt-buckles. Punch biopsies performed in 7 patients showed subacute dermatitis. After avoidance of continual exposure to objects containing nickel and foods rich in nickel, as well as treatment with oral antihistamines and topical corticosteroids, all patients improved or cleared. It has been reported that nickel can cause systemic contact dermatitis by some internal systemic route, such as oral intake, transfusion, inhalation, implantation of metal medical devices, etc. In our patients, we found that continual local skin contact could also elicit systemic contact dermatitis.
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http://dx.doi.org/10.1034/j.1600-0536.2003.00017.xDOI Listing
March 2003
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