Publications by authors named "Xi-Zhe Li"

2 Publications

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sp. nov., isolated from the production water of a water-flooded oil reservoir in PR China.

Int J Syst Evol Microbiol 2020 Jul;70(7):4364-4371

State Key Laboratory of Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.

A novel, moderately thermophilic, Gram-stain-negative bacterium, designated strain J18, was isolated from a water-flooded oil reservoir. Cells were aerobic, oxidase- and catalase-positive, with a polar flagellum. Growth occurred at 35-60 °C and at pH 6-8.5. The respiratory quinones were ubiquinone 8 and ubiquinone 9. The dominant cellular fatty acids were C, C cyclo, C cyclo 8 and summed feature 8 (C7/C6). The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and an unidentified aminophospholipid. The strain showed the highest 16S rRNA gene sequence similarities to DSM 15129 (98.6 %), DSM 15512 (98.4 %) and DSM 27220 (98.1 %), respectively, and the similarity to other species was lower than 93 %. In the phylogenetic trees, it constituted a unique sub-cluster within the genus . The DNA G+C content of strain J18 was 64.44 mol%. As compared with the type strains, the genome-to-genome distances of strain J18 were 34.7-40 %. These results confirmed the separate species status of J18 with its close relatives. On the basis of physiological, chemotaxonomic and phylogenetic analyses along with the low levels of identity at the whole-genome level, it can be concluded that strain J18 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain of is J18 (=CGMCC 1.16800=LMG 31400).
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http://dx.doi.org/10.1099/ijsem.0.004297DOI Listing
July 2020

gen. nov., sp. nov., a new member of the family isolated from oil reservoir water.

Int J Syst Evol Microbiol 2020 May;70(5):3468-3474

State Key Laboratory of Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.

A novel Gram-staining-negative, spiral-shaped bacterium, designated strain 64-1, was isolated from oil reservoir water collected from Liaohe oilfield, north-eastern China. Growth occurred at 15-55 °C and pH 6.0-10.0. The sole respiratory quinone was Q-10. The predominant cellular fatty acids were summed feature 8 (C7 /C6), C and C cyclo 8. The polar lipids consisted of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), an unidentified aminophospholipid (UAPL), an unidentified aminolipid (UAL) and two unidentified polar lipids (UPL). The genomic DNA G+C content of strain 64-1 was 64.5 mol%. Strain 64-1 shared the highest 16S rRNA gene sequence similarities with JA145 (92.0 %) and 26-4b1 (91.8 %). In the phylogenetic trees, the strain constituted a sub-cluster within the family . Based on the results of morphological, physiological, biochemical and phylogenetic analysis, strain 64-1 represents a new species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is 64-1 (=CGMCC 1.16798=LMG 31399).
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http://dx.doi.org/10.1099/ijsem.0.004200DOI Listing
May 2020