Publications by authors named "Xi-Ming Wang"

59 Publications

The Mechanism of as a Treatment for Hyperlipidemia Based on Network Pharmacology and Experimental Validation.

Evid Based Complement Alternat Med 2022 13;2022:5821829. Epub 2022 Apr 13.

Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.

Materials And Methods: The active compounds in DO, their targets, and targets associated with hyperlipidemia were screened across various databases, and the hidden targets of DO in treating hyperlipidemia were forecast. The compound-target (C-T), protein-protein interaction (PPI), and compound-target-pathway (C-T-P) networks of DO were set up with Cytoscape software. The hub genes and core clusters of DO predicted to be active against hyperlipidemia were calculated by Cytoscape. The DAVID database was adopted for Gene Ontology (GO) analysis and KEGG pathway enrichment analysis. Next, we used the high-sucrose-fat diet and alcohol (HFDA)-induced hyperlipidemia rats to evaluate the hypolipidemic effect of DO.

Results: In this study, we obtained 264 compounds from DO, revealed 11 bioactive compounds, and predicted 89 potential targets of DO. The network analysis uncovered that naringenin, isorhamnetin, and taxifolin might be the compounds in DO that are mainly in charge of its roles in hyperlipidemia and might play a role by modulating the targets (including PPARG, ADIPOQ, AKT1, TNF, and APOB). The pathway analysis showed that DO might affect diverse signaling pathways related to the pathogenesis of hyperlipidemia, including PPAR signaling pathway, insulin resistance, AMPK signaling pathway, and non-alcoholic fatty liver disease simultaneously. Meanwhile, in the HFDA-induced hyperlipidemia rat model, DO could significantly decrease the level of TC, TG, LDL-c, and ALT in serum, and increase HDL-c as well. The liver pathological section indicated that DO could ease liver damage and lipid cumulation.

Conclusion: In summary, the biological targets of the main bioactive compounds in DO were found to distribute across multiple metabolic pathways. These findings suggest that a mutual regulatory system consisting of multiple components, targets, and pathways is a likely mechanism through which DO may improve hyperlipidemia. Validation experiments indicated that DO may treat hyperlipidemia by affecting NAFLD-related signaling pathways.
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http://dx.doi.org/10.1155/2022/5821829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9056230PMC
April 2022

New model of PIRADS and adjusted prostatespecific antigen density of peripheral zone improves the detection rate of initial prostate biopsy: a diagnostic study.

Asian J Androl 2022 Apr 22. Epub 2022 Apr 22.

Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.

This study explored a new model of Prostate Imaging Reporting and Data System (PIRADS) and adjusted prostate-specific antigen density of peripheral zone (aPSADPZ) for predicting the occurrence of prostate cancer (PCa) and clinically significant prostate cancer (csPCa). The demographic and clinical characteristics of 853 patients were recorded. Prostate-specific antigen (PSA), PSA density (PSAD), PSAD of peripheral zone (PSADPZ), aPSADPZ, and peripheral zone volume ratio (PZ-ratio) were calculated and subjected to receiver operating characteristic (ROC) curve analysis. The calibration and discrimination abilities of new nomograms were verified with the calibration curve and area under the ROC curve (AUC). The clinical benefits of these models were evaluated by decision curve analysis and clinical impact curves. The AUCs of PSA, PSAD, PSADPZ, aPSADPZ, and PZ-ratio were 0.669, 0.762, 0.659, 0.812, and 0.748 for PCa diagnosis, while 0.713, 0.788, 0.694, 0.828, and 0.735 for csPCa diagnosis, respectively. All nomograms displayed higher net benefit and better overall calibration than the scenarios for predicting the occurrence of PCa or csPCa. The new model significantly improved the diagnostic accuracy of PCa (0.945 vs 0.830, P < 0.01) and csPCa (0.937 vs 0.845, P < 0.01) compared with the base model. In addition, the number of patients with PCa and csPCa predicted by the new model was in good agreement with the actual number of patients with PCa and csPCa in high-risk threshold. This study demonstrates that aPSADPZ has a higher predictive accuracy for PCa diagnosis than the conventional indicators. Combining aPSADPZ with PIRADS can improve PCa diagnosis and avoid unnecessary biopsies.
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http://dx.doi.org/10.4103/aja202218DOI Listing
April 2022

Patients with Earlobe Crease May Associate with Lower Concentration of the Age-Suppressing Hormone Klotho.

Int J Gen Med 2021 25;14:8797-8803. Epub 2021 Nov 25.

Department of Radiology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250021, People's Republic of China.

Objective: Earlobe crease (ELC) has been considered as a skin sign of atherosclerosis, and its pathophysiological mechanism is still unclear. Our study aims to test the hypothesis that ELC patients with lower serum levels of the age-suppressing hormone Klotho, which is not only associated with premature aging but also with endothelial dysfunction, may be associated with atherosclerosis.

Methods: A total of 135 patients aged 40-68 years underwent coronary angiography. According to the presence or absence of coronary heart disease (CAD) and ELC, they were divided into three groups: CAD group and ELC group (ELC group, n = 45); no ELC group (non-ELC group, n = 45). There was no ELC or CAD in the control group (control group, n = 45). Serum Klotho concentration was obtained by enzyme-linked immunosorbent assay (ELISA).

Results: The Klotho level in the ELC group was 365.6 ± 38.1 pg/mL, while the Klotho level in the non-ELC group was 568.8 ± 44.9 pg/mL. It is worth noting that the Klotho level of the ELC group was significantly lower than that of the non-ELC group (P < 0.001). The serum Klotho level of the control group was higher than that of the non-ELC group (593.3±45.3 vs 568.8±44.9 pg/mL, P = 0.702), but the difference was not statistically significant. Multiple logistic regression analysis showed that the Klotho level is a parameter that affects the appearance of ELC.

Conclusion: Serum Klotho levels were considerably lower in patients with ELC. We concluded that the perturbations of Klotho in patients might be associated with ELC and with CAD.
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http://dx.doi.org/10.2147/IJGM.S300309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629917PMC
November 2021

Coronary artery calcification and risk of mortality and adverse outcomes in patients with COVID-19: a Chinese multicenter retrospective cohort study.

Chin J Acad Radiol 2022 28;5(1):20-28. Epub 2021 Jun 28.

Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, 305 Zhongshan East Road, Xuanwu District, Nanjing, 210002 Jiangsu Province People's Republic of China.

Background: Coronary artery calcification (CAC) is an independent risk factor of major adverse cardiovascular events; however, the impact of CAC on in-hospital death and adverse clinical outcomes in patients with coronavirus disease 2019 (COVID-19) remains unclear.

Objective: To explore the association between CAC and in-hospital mortality and adverse events in patients with COVID-19.

Methods: This multicenter retrospective cohort study enrolled 2067 laboratory-confirmed COVID-19 patients with definitive clinical outcomes (death or discharge) admitted from 22 tertiary hospitals in China between January 3, 2020 and April 2, 2020. Demographic, clinical, laboratory results, chest CT findings, and CAC on admission were collected. The primary outcome was in-hospital death and the secondary outcome was composed of in-hospital death, admission to intensive care unit (ICU), and requiring mechanical ventilation. Multivariable Cox regression analysis and Kaplan-Meier plots were used to explore the association between CAC and in-hospital death and adverse clinical outcomes.

Results: The mean age was 50 years (SD,16) and 1097 (53.1%) were male. A total of 177 patients showed high CAC level, and compared with patients with low CAC, these patients were older (mean age: 49 vs. 69 years,  < 0.001) and more likely to be male (52.0% vs. 65.0%,  = 0.001). Comorbidities, including cardiovascular disease (CVD) ([33.3%, 59/177] vs. [4.7%, 89/1890],  < 0.001), presented more often among patients with high CAC, compared with patients with low CAC. As for laboratory results, patients with high CAC had higher rates of increased D-dimer, LDH, as well as CK-MB (all  < 0.05). The mean CT severity score in high CAC group was also higher than low CAC group (12.6 vs. 11.1,  = 0.005). In multivariable Cox regression model, patients with high CAC were at a higher risk of in-hospital death (hazard ratio [HR], 1.731; 95% CI 1.010-2.971,  = 0.046) and adverse clinical outcomes (HR, 1.611; 95% CL 1.087-2.387,  = 0.018).

Conclusion: High CAC is a risk factor associated with in-hospital death and adverse clinical outcomes in patients with confirmed COVID-19, which highlights the importance of calcium load testing for hospitalized COVID-19 patients and calls for attention to patients with high CAC.

Supplementary Information: The online version contains supplementary material available at 10.1007/s42058-021-00072-4.
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http://dx.doi.org/10.1007/s42058-021-00072-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237549PMC
June 2021

Integration of clinicopathologic identification and deep transferrable image feature representation improves predictions of lymph node metastasis in prostate cancer.

EBioMedicine 2021 Jun 25;68:103395. Epub 2021 May 25.

Department of Radiology, The First Affiliated Hospital of Nanjing Medical University; Nanjing, Jiangsu Province, PR China. Electronic address:

Background: Accurate identification of pelvic lymph node metastasis (PLNM) in patients with prostate cancer (PCa) is crucial for determining appropriate treatment options. Here, we built a PLNM-Risk calculator to obtain a precisely informed decision about whether to perform extended pelvic lymph node dissection (ePLND).

Methods: The PLNM-Risk calculator was developed in 280 patients and verified internally in 71 patients and externally in 50 patients by integrating a set of radiologists' interpretations, clinicopathological factors and newly refined imaging indicators from MR images with radiomics machine learning and deep transfer learning algorithms. Its clinical applicability was compared with Briganti and Memorial Sloan Kettering Cancer Center (MSKCC) nomograms.

Findings: The PLNM-Risk achieved good diagnostic discrimination with areas under the receiver operating characteristic curve (AUCs) of 0.93 (95% CI, 0.90-0.96), 0.92 (95% CI, 0.84-0.97) and 0.76 (95% CI, 0.62-0.87) in the training/validation, internal test and external test cohorts, respectively. If the number of ePLNDs missed was controlled at < 2%, PLNM-Risk provided both a higher number of ePLNDs spared (PLNM-Risk 59.6% vs MSKCC 44.9% vs Briganti 38.9%) and a lower number of false positives (PLNM-Risk 59.3% vs MSKCC 70.1% and Briganti 72.7%). In follow-up, patients stratified by the PLNM-Risk calculator showed significantly different biochemical recurrence rates after surgery.

Interpretation: The PLNM-Risk calculator offers a noninvasive clinical biomarker to predict PLNM for patients with PCa. It shows improved accuracy of diagnosis support and reduced overtreatment burdens for patients with findings suggestive of PCa.

Funding: This work was supported by the Key Research and Development Program of Jiangsu Province (BE2017756) and the Suzhou Science and Technology Bureau-Science and Technology Demonstration Project (SS201808).
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http://dx.doi.org/10.1016/j.ebiom.2021.103395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167242PMC
June 2021

Optimized MRI Assessment for Clinically Significant Prostate Cancer: A STARD-Compliant Two-Center Study.

J Magn Reson Imaging 2021 04 19;53(4):1210-1219. Epub 2020 Oct 19.

Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background: There is a requirement for a personalized strategy to make MRI more accessible to men with suspicion of clinically significant prostate cancer (CSPC).

Purpose: To evaluate an optimized (Op)-MRI compared with biparametric (Bp)-MRI and multiparametric (Mp)-MRI for the diagnosis of CSPC.

Study Type: Two-center, retrospective.

Subjects: A total of 346 patients from center 1 and 292 patients from center 2.

Field Strength/sequence: 3.0T scanners, T -weighted imaging (T WI), diffusion-weighted imaging (DWI), and dynamic contrast-enhanced (DCE) imaging.

Assessment: Four radiologists interpreted the Bp-MRI (T WI and DWI) and Mp-MRI (T WI, DWI, and DCE) independently according to the Prostate Imaging Reporting and Data System (PI-RADS). For Op-MRI, two radiologists used an adjusted decision rule on Bp-MRI-assessed PI-RADS 3 lesions by determining early enhancement of DCE. Pathologies at biopsy and/or prostatectomy specimens were used as standard references.

Statistical Tests: Performance was assessed using receiver operating characteristic (ROC) curves. Kappa statistics were used to assess interobserver variability.

Results: Interreader agreement was excellent for all three MRI assessments (all κ values >0.80). Op-MRI had comparable sensitivity (senior/junior: 90.9% [261/287] / 91.6% [263/287]) and higher specificity (78.1% [274/351] /74.4% [261/351]) compared with Mp-MRI (sensitivity: 92.3% [265/287] / 93.7% [269/287]; specificity: 67.8% [238/351] / 68.1% [239/351]) and Bp-MRI (sensitivity: 91.6% [263/287] / 93.4% [268/287]; specificity: 71.2% [250/351] / 70.1% [246/351]) for the diagnosis of CSPC. Compared to Mp-MRI, Op-MRI spared biopsy in 80.7% (515/638) of DCE scans with similar performance accuracy. Compared to Bp-MRI, Op-MRI downgraded 25.2% (31/123) of lesions at a cost of missing 6.5% (3/46) of malignancies, and upgraded 45.5% (56/123) of lesions with a positive predictive value of 62.5% (35/56) in 123 equivocal findings.

Data Conclusion: The Op-MRI, using an adjusted PI-RADS decision rule, did not compromise diagnostic accuracy with sparing biopsy in 80.7% of DCE scans compared to Mp-MRI, and outperformed Bp-MRI by regrading PI-RADS lesions.

Level Of Evidence: 4 TECHNICAL EFFICACY STAGE: 2.
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http://dx.doi.org/10.1002/jmri.27394DOI Listing
April 2021

Left atrial appendage amputation using a modified appendage clip: an experimental canine study.

Chin Med J (Engl) 2020 Nov;133(21):2644-2646

Department of Radiology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, China.

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http://dx.doi.org/10.1097/CM9.0000000000001122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722599PMC
November 2020

Boron-Modified Defect-Rich Molybdenum Disulfide Nanosheets: Reducing Nonspecific Adsorption and Promoting a High Capacity for Isolation of Immunoglobulin G.

ACS Appl Mater Interfaces 2020 Sep 10;12(38):43273-43280. Epub 2020 Sep 10.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.

A new type of boric acid derivative-modified molybdenum disulfide nanosheet was prepared by amination and sulfur chemical grafting, where lipoic acid, lysine, and 5-carboxybenzoboroxole were used as reactants. The two-dimensional composite, abbreviated as MoS-Lys-CBX, is an ultrathin nanosheet with a minimum unit of single or few layers. Compared with the original molybdenum disulfide, the nonspecific adhesion of interfering proteins on the surface was reduced, and the adsorption capacity of glycoproteins was enhanced, which was 1682.2 mg g represented by IgG. The adsorbed IgG can be easily eluted with 0.3 wt % CTAB with an elution efficiency of 94.1%. Circular dichroism spectra indicate no obvious conformation change of IgG during the purification process by the MoS-Lys-CBX nanosheets. The as-prepared MoS-Lys-CBX nanosheets were then employed for the isolation of IgG from human serum sample, obtaining high-purity light and heavy chains of IgG, as demonstrated by SDS-PAGE assays.
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http://dx.doi.org/10.1021/acsami.0c12171DOI Listing
September 2020

Anomalous systemic artery to the non-sequestrated lung: multi-slice computed tomography features.

Chin Med J (Engl) 2020 Sep;133(18):2259-2260

Department of Radiology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, China.

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http://dx.doi.org/10.1097/CM9.0000000000000965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508426PMC
September 2020

Risk factors for adverse clinical outcomes with COVID-19 in China: a multicenter, retrospective, observational study.

Theranostics 2020 15;10(14):6372-6383. Epub 2020 May 15.

Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, 210002, China.

The risk factors for adverse events of Coronavirus Disease-19 (COVID-19) have not been well described. We aimed to explore the predictive value of clinical, laboratory and CT imaging characteristics on admission for short-term outcomes of COVID-19 patients. This multicenter, retrospective, observation study enrolled 703 laboratory-confirmed COVID-19 patients admitted to 16 tertiary hospitals from 8 provinces in China between January 10, 2020 and March 13, 2020. Demographic, clinical, laboratory data, CT imaging findings on admission and clinical outcomes were collected and compared. The primary endpoint was in-hospital death, the secondary endpoints were composite clinical adverse outcomes including in-hospital death, admission to intensive care unit (ICU) and requiring invasive mechanical ventilation support (IMV). Multivariable Cox regression, Kaplan-Meier plots and log-rank test were used to explore risk factors related to in-hospital death and in-hospital adverse outcomes. Of 703 patients, 55 (8%) developed adverse outcomes (including 33 deceased), 648 (92%) discharged without any adverse outcome. Multivariable regression analysis showed risk factors associated with in-hospital death included ≥ 2 comorbidities (hazard ratio [HR], 6.734; 95% CI; 3.239-14.003, p < 0.001), leukocytosis (HR, 9.639; 95% CI, 4.572-20.321, p < 0.001), lymphopenia (HR, 4.579; 95% CI, 1.334-15.715, p = 0.016) and CT severity score > 14 (HR, 2.915; 95% CI, 1.376-6.177, p = 0.005) on admission, while older age (HR, 2.231; 95% CI, 1.124-4.427, p = 0.022), ≥ 2 comorbidities (HR, 4.778; 95% CI; 2.451-9.315, p < 0.001), leukocytosis (HR, 6.349; 95% CI; 3.330-12.108, p < 0.001), lymphopenia (HR, 3.014; 95% CI; 1.356-6.697, p = 0.007) and CT severity score > 14 (HR, 1.946; 95% CI; 1.095-3.459, p = 0.023) were associated with increased odds of composite adverse outcomes. The risk factors of older age, multiple comorbidities, leukocytosis, lymphopenia and higher CT severity score could help clinicians identify patients with potential adverse events.
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http://dx.doi.org/10.7150/thno.46833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255028PMC
June 2020

Purification of hemoglobin by adsorption on nitrogen-doped flower-like carbon superstructures.

Mikrochim Acta 2020 02 11;187(3):162. Epub 2020 Feb 11.

Department of Chemistry, Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.

Nitrogen-doped flower-like carbon superstructures (NPC-F) are prepared via carbonizing self-assembled polyimide nanosheets. SEM, TEM, XPS, and N sorption methods are adopted to characterize the flower-like structure. NPC-F exhibits adsorption selectivity for hemoglobin (Hb) because the specific pyridinic N groups of NPC-F could coordinate with the sixth vacancy of ferrous ion in hemoglobin. The adsorption behavior fits well with Langmuir model with a maximum adsorption capacity of 360.0 mg g and the adsorbed Hb could be lightly stripped from the NPC-F nanospheres surface by 0.5 wt% CTAB solution. Circular dichroism spectra indicate no obvious conformation changing of Hb during purification process by NPC-F nanospheres. Five cycles of a continuous adsorption/desorption experiment demonstrate the reusability of NPC-F as adsorbent for Hb. The prepared NPC-F superstructures are then employed for the isolation of Hb from human whole blood sample, obtaining high-purity Hb as demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis assays. Graphical abstractNitrogen-doped flower-like carbon superstructure (NPC-F) is used to isolate target protein. NPC-F exhibits highly selective capture capacity towards hemoglobin because the specific pyridinic N groups of NPC-F could coordinate with the sixth vacant coordinating position of Fe in hemoglobin.
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http://dx.doi.org/10.1007/s00604-020-4151-9DOI Listing
February 2020

Two-dimensional titanate-based zwitterionic hydrophilic sorbent for the selective adsorption of glycoproteins.

Anal Chim Acta 2019 Dec 20;1088:72-78. Epub 2019 Aug 20.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China. Electronic address:

The selective adsorption towards glycoproteins from complex biosamples is of vital importance in life science studies. A new zwitterionic hydrophilic material, i.e., a functionalized titanate nanosheet, is prepared by assembling well-dispersed gold nanoparticles (AuNPs) on the surface of ultrathin titanate nanosheets via an ion-exchange approach, followed through immobilizing l-cysteine (L-Cys) by Au-S bonding. This 2D-titanate-based zwitterionic hydrophilic material is shortly termed as L-Cys/Au/TiNSs and it exhibits transverse several hundred nanometers with an ultrathin nanosheet structure. The zwitterionic hydrophilic titanate nanosheets have strong adsorption affinity to glycoproteins, offering a large binding capacity towards immunoglobulin G (1098.9 mg g), which could be readily stripped into an ammonium hydroxide (NHOH) solution (0.5%, m/v) with a recovery of 82.4%. The practical applications of L-Cys/Au/TiNSs are further proved by successful specific adsorption of IgG from human serum.
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http://dx.doi.org/10.1016/j.aca.2019.08.041DOI Listing
December 2019

DMSA-Functionalized Mesoporous Alumina with a High Capacity for Selective Isolation of Immunoglobulin G.

ACS Appl Mater Interfaces 2019 Oct 19;11(39):36286-36295. Epub 2019 Sep 19.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences , Northeastern University , Box 332, Shenyang 110819 , China.

A novel dimercaptosuccinic acid-functionalized mesoporous alumina (DMSA-MA) is synthesized by the dicarboxylic acid groups of dimercaptosuccinic acid molecules coordinating to the Al ions located in the mesostructure. The as-prepared DMSA-MA composites possess a large surface area of 91.17 m/g as well as a uniform pore size and a high pore volume of 17.22 nm and 0.23 cm/g, respectively. DMSA coating of mesostructures significantly enhanced their selectivity for glycoprotein adsorption through a powerful hydrophilic binding force, and the maximum adsorption capacity of immunoglobulin G (IgG) can reach 2298.6 mg g. The captured IgG could be lightly stripped from the DMSA-MA composites with an elution rate of 98.3% by using 0.5 wt % CTAB solution as the elution reagent. DMSA-MA is further employed as a sorbent for the enrichment of IgG heavy chain and light chain from human serum sample. SDS-PAGE assay results showed the obtained IgG with high purity compared to that of the standard solution of IgG.
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http://dx.doi.org/10.1021/acsami.9b13718DOI Listing
October 2019

Boron-titanate monolayer nanosheets for highly selective adsorption of immunoglobulin G.

Nanoscale 2019 May;11(19):9362-9368

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.

Boron-titanate monolayer nanosheets were prepared through a scalable step by step intercalation approach for anchoring 3-mercaptopropyltriethoxysilane (MPTS) on the surface. MPTS provides clickable sites with 4-vinylphenylboronic acid (VPBA) via a thiol-ene (TE) click reaction to obtain monolayer titanate nanosheets with boronic acid ligands immobilized on the surface. The nanosheets obtained are denoted as VPBA-MPTS-TiNSs, with a lateral dimension of a few dozen nanometers and with a thickness of ca. 3.5 nm. The nanosheets exhibit a superior adsorption capacity of 1669.7 mg g-1 and favorable selectivity for the adsorption of glycoproteins by employing immunoglobulin G (IgG) as the protein model. The adsorbed IgG is thereafter readily collected by using 0.1% (m/v) cetane trimethyl ammonium bromide (CTAB) as the eluent. The practical applications of VPBA-MPTS-TiNSs are further demonstrated by the selective adsorption/purification of IgG from human serum.
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http://dx.doi.org/10.1039/c9nr01111kDOI Listing
May 2019

p38 MAPK is Crucial for Wnt1- and LiCl-Induced Epithelial Mesenchymal Transition.

Curr Med Sci 2018 Jun 22;38(3):473-481. Epub 2018 Jun 22.

Department of Pulmonary Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.

Idiopathic pulmonary fibrosis (IPF) is characterized by myofibroblast foci in lung parenchyma. Myofibroblasts are thought to originate from epithelial-to-mesenchymal transition (EMT). Wnt1 and lithium chloride (LiCl) induce EMT in alveolar epithelial cells (AECs), but the mechanisms are unclear. AECs were treated with Wnt1 and LiCl, respectively; morphological change and molecular changes of EMT, including E-cadherin, fibronectin, and vimentin, were observed. SB203580 was administrated to test the role of p38 МАРК signaling in EMT. Then AECs were treated with siRNAs targeting p38 МАРК to further test the effects of p38 МАРК, and the role was further confirmed by re-expression of p38 МАРК. At last P-catenin siRNA was used to test the role of β-catenin in the EMT process and relationship of β-catenin and p38 МАРК was concluded. Exposure of AECs to Wnt1 and LiCl resulted in upregulation of vimentin and fibronectin with subsequent downregulation of E-cadherin. Wnt1 and LiCl stimulated the p38 МАРК signaling pathways. Perturbing the p38 МАРК pathway either by SB203580 or through p38 МАРК siRNA blocked EMT and inhibited fibronetin synthesis, which were reversed by transfection of p38 МАРК expression plasmid. β-catenin siRNA attenuated the EMT process and decreased p38 МАРК phosphorylation, indicating that β-catenin is involved in the EMTrelated changes through regulation of p38 МАРК phosphorylation. These findings suggest that p38 МАРК participates in the pathogenesis of EMT through Wnt pathway and that p38 МАРК may be a novel target for IPF therapy.
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http://dx.doi.org/10.1007/s11596-018-1903-4DOI Listing
June 2018

ZrO doped magnetic mesoporous polyimide for the efficient enrichment of phosphopeptides.

Talanta 2018 Oct 24;188:385-392. Epub 2018 May 24.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address:

FeO and ZrO nanoparticles co-doped layered porous polyimide, polyimide-FeO-ZrO is prepared with a one-step strategy, shortly termed as PI-FeO-ZrO. The layered and porous structure of the polymer offers a supported platform for metallic oxide anchoring, exhibiting a mesopore size of 3.93 nm and providing a surface area of 198.47 m g. The metallic oxides were uniformly and highly dispersed in the PI-FeO-ZrO nanocomposite with percentages of 15.81 and 20.53 wt% for Fe and Zr, respectively. The magnetic FeO provides driving force for rapid separation. The high doping of ZrO facilitates effective enrichment of phosphopeptides, even at a very low mass ratio of 1:1000 for tryptic digest of phosphopeptides/non-phosphopeptides, e.g., β-casein/BSA in this particular case. In addition, the PI-FeO-ZrO nanocomposite exhibits better adsorption performance to phosphopeptides with respect to commercial titanium dioxide nanoparticles. The effectiveness of low-abundant phosphopeptides isolation and enrichment from human serum is further identified and demonstrated by means of MALDI-TOF MS and LC-ESI-MS/MS.
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http://dx.doi.org/10.1016/j.talanta.2018.05.069DOI Listing
October 2018

PEGylated titanate nanosheets: hydrophilic monolayers with a superior capacity for the selective isolation of immunoglobulin G.

Nanoscale 2018 Jul;10(26):12535-12542

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.

A novel organic-inorganic hybrid was prepared by anchoring (3-aminopropyl)triethoxysilane (APTES) on the surface of monolayer titanate nanosheets and subsequent modification with hydrophilic polyethylene glycol (PEG). The PEGylated hydrophilic monolayer titanate nanosheets were abbreviated as PEG-APTES-TiNSs, and they exhibit a lateral dimension of dozens of nanometers and a thickness of ca. 1.9 nm. PEGylation of the titanate nanosheets significantly improved their selectivity toward the adsorption of glycoproteins through strong hydrophilic interaction, providing an adsorption capacity of 2540.9 mg g-1 for immunoglobulin G (IgG). The retained IgG is readily collected at a recovery rate of 83.4% with 0.5% (m/v) ammonium hydroxide (NH4OH) as the stripping reagent. PEG-APTES-TiNSs are applied for the selective adsorption of IgG from human serum, which is further confirmed by SDS-PAGE assay.
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http://dx.doi.org/10.1039/c8nr02995dDOI Listing
July 2018

An association of spleen volume and aortic diameter in patients and in mice with abdominal aortic aneurysm.

BMC Surg 2017 Dec 15;17(1):134. Epub 2017 Dec 15.

Department of Vascular Surgery, Peking Union Medical College Hospital, No.1 Shuaifuyuan, Dongdan, Dongcheng District, Beijing, 100730, China.

Background: To investigate the potential mechanism of splenic enlargement in Ang II/APOE model and the associations between the spleen volume and the indices of abdominal aortic aneurysm (AAA) in human.

Methods: To investigate the changes of spleen volume on AAA formation, apolipoprotein E knockout (Apo E) mice were treated with Ang II (1000 ng/kg/min) up to 28 days to generate AAA. We used Magnetic Resonance Imaging (MRI), liquid measurement, H&E and immunohistochemistry to analyze the morphological or pathological changes of spleen. To investigate the changes of spleen volume in human, a retrospective case-control study involving 30 male AAA patients and 25 male controls were performed. Spleen volume was measured on computed tomography images. Univariate analysis and multivariable sequential logistic regression analyses were used to analyze the association between spleen volume and maximal diameter (Dmax).

Results: In Ang II/APOE model, we found splenic enlargement in mice with AAA compared with the sham group. Histopathological investigations revealed hypertrophies of splenic follicles and increased populations of CD3 T cells. In clinic cohort study, univariate analysis revealed higher values in large AAA (Dmax > 5.5 cm,n = 15) compared with the small (Dmax < 5.5 cm,n = 15) for spleen volume (230.6 ± 64.5 cm vs. 170.0 ± 32.8 cm; P = 0.0030). Regression analysis revealed a statistically significant positive linear correlation of spleen volume and Dmax of AAA (r = 0.3611;P = 0.0423).

Conclusions: Mimicking the splenic pathology observed in murine AAA model, there is a strong positive correlation between spleen volume and the Dmax in male AAA patients. As Dmax is a valuable predictor of AAA rupture, the spleen enlargement may be another indicator.
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http://dx.doi.org/10.1186/s12893-017-0328-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731055PMC
December 2017

The long non-coding RNA NONHSAT062994 inhibits colorectal cancer by inactivating Akt signaling.

Oncotarget 2017 Sep 2;8(40):68696-68706. Epub 2017 Aug 2.

Department of Pathology, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China.

The aberrant expression of long noncoding RNAs (lncRNAs) is implicated in cancer development and progression. However, the clinical significance and mechanism by which NONHSAT062994 regulates colorectal cancer (CRC) is unknown. We here reported that NONHSAT062994 was significantly downregulated in human CRC tissues and cell lines. Moreover, its expression was inversely correlated with tumor size and overall survival (OS) time in CRC patients. In CRC cells, the overexpression and knockdown of NONHSAT062994 inhibited and enhanced CRC cell growth, respectively, and . Mechanistically, NONHSAT062994 functioned as a tumor suppressor to inhibit CRC cell growth by inactivating Akt signaling. Notably, the NONHSAT062994 expression status was negatively correlated with the Akt downstream targets c-Myc and Cyclin D1 in clinical CRC samples. The current findings suggest that NONHSAT062994 plays a critical role in the development of CRC by regulating Akt signaling, and identified a candidate prognostic biomarker or potential therapeutic target for CRC patients.
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http://dx.doi.org/10.18632/oncotarget.19827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620289PMC
September 2017

Perioperative safety analysis of transcatheter arterial chemoembolization for hepatocellular carcinoma patients with preprocedural leukopenia or thrombocytopenia.

Mol Clin Oncol 2017 Sep 25;7(3):435-442. Epub 2017 Jul 25.

Department of Interventional Radiology, Beijing 302 Hospital, Beijing 100039, P.R. China.

Patients with hepatocellular carcinoma (HCC) exhibit a high incidence of concomitant cirrhosis with leukopenia and/or thrombocytopenia. In the present study, perioperative changes in the white blood cell (WBC) and platelet (PLT) counts and associated complications were investigated to assess the safety of transcatheter arterial chemoembolization (TACE) for HCC patients with preprocedural leukopenia or thrombocytopenia. The records of 1,461 HCC patients who received TACE between January 2012 and December 2013 were retrospectively reviewed. The incidence of complications during the perioperative period and changes in the WBC and PLT counts were recorded. A Chi-squared test was used to evaluate the associations between postoperative infection and preprocedural WBC count and between bleeding at the puncture site and preprocedural PLT count. The WBC count of the majority of the patients increased within 3 days and returned to the preprocedural level within 30 days after TACE. The PLT count decreased within 3 days and returned to the preprocedural level within 30 days after TACE. The major complications were liver decompensation (n=66), puncture site bleeding (n=45), infection (n=33), severe thrombocytopenia (n=8), upper gastrointestinal bleeding (n=6), tumor bleeding (n=4) and agranulocytosis (n=3). A Chi-squared test revealed that postoperative infection was not associated with preprocedural WBC count and puncture site bleeding was not associated with decreased PLT count due to hypersplenism. Therefore, TACE was found to be safe for HCC patients with preprocedural thrombocytopenia or leukopenia due to hypersplenism, with a low incidence of major complications during the perioperative period.
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http://dx.doi.org/10.3892/mco.2017.1345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547764PMC
September 2017

Penile metastases from primary lung cancer: Case report and literature review.

Medicine (Baltimore) 2017 Jun;96(26):e7307

Department of Pathology Department of Urology Department of Radiology Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Metastasis to the penis from primary lung cancer is quite rare. To improve the understanding, we present a case diagnosed as penile metastasis from primary lung cancer and review the literature.

Methods: One case report and retrospectively analysis penile cancer patient secondary from primary lung cancer.

Results: The patient complained of perineal pain and burning on urination for about 2 months. On physical examination, painful nodular masses at the base of left side of the corpora cavernosa were found. 18F-fluorodeoxyglucose positron emission tomography/CT (PET/CT) scan showed that maximum standardized uptake value (SUVmax) in left side corpora cavernosa and right hilar increased to 12.0 and 13.5 respectively. On flexible bronchoscopy checking, stenosis of the opening of apical segmental and posterior segmental bronchi of right upper lobe was found. The lateral segmental bronchi of left lower lobe was obstructed by a neoplasm. The pathological result was primary pulmonary adenosquamous carcinoma (ASC). Two months later, total penectomy was performed. The pathological result was penile ASC derived from pulmonary. On reviewing the literature, there are 39 cases reported. The patient we present is the 40 one. The average age at diagnosis was (60.5 ± 10.7) years old. The most common symptom was mass, followed by priapism, pain. The overall survival time was (4.5 ± 3.9) months.

Conclusions: The penis may be a site of metastasis from lung cancer, especially for old patient. Metastasis to the penis usually indicates that the primary lung cancer is at an advanced stage and the prognosis is very poor.
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http://dx.doi.org/10.1097/MD.0000000000007307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500058PMC
June 2017

Enhanced radiation damage caused by iodinated contrast agents during CT examination.

Eur J Radiol 2017 Jul 10;92:72-77. Epub 2017 Apr 10.

Department of Radiology, The First Affiliated Hospital of Soochow University, No. 188, Street of Ten Catalpa, Suzhou, 200000, China. Electronic address:

Objective: To access the effect of iodinate contrast agent (ICA) on DNA double-stand breaks (DSBs) in human peripheral blood lymphocytes during computed tomography (CT) examinations.

Materials And Methods: This present study was approved by the institutional ethics committee; written informed patient consent was obtained from 70 patients. A total of 48 patients underwent computed tomography urography (CTU), in which only one time CT scanning was examined after injecting ICA, and 22 patients received unenhanced whole abdominal CT, among them 10 patients were selected to get ICA injection immediately after irradiation. Blood samples were taken from all patients prior to and immediately after CT scan, as well as 8min after the injection of ICA. The lymphocytes in these blood samples were separated by using density-gradient centrifugation, fixed and immunostained with γH2AX antibody. The average number of phosphorylated histone H2AX (γH2AX) foci per lymphocyte was counted under a fluorescence microscopy. Differences in the number of γH2AX-foci were statistically analyzed using independent sample t test and one way ANOVA.

Result: The three patient groups had no significant differences in the baseline foci numbers(P>0.05). The γH2AX-focus levels increased in both groups after CT scan. Patients who underwent CTU examinations had a greater DSBs level (mean±standard error of mean, 0.945±0.184 foci per cell) than those who received unenhanced whole abdominal CT scan (mean±standard error of mean, 0.700±0.112 foci per cell), increasing by about 37.9%; The ICA injected before CT scan itself had an effect on the DSBs, which increased DSBs level by approximately 90.3% (0.059±0.018vs 0.031±0.025, P<0.05), but no significant difference was found if added after irradiation, increasing DSBs level only by 3.2% approximately (0.711±0.091vs 0.689±0.108, P=0.499).

Conclusion: The iodinated contrast agent itself can lead to an increase in the level of DSBs as assessed with γH2AX foci formation, and the application of ICA can amplify DNA damage induced by diagnostic x-ray procedures such as whole abdominal CT.
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http://dx.doi.org/10.1016/j.ejrad.2017.04.005DOI Listing
July 2017

Surgery combined with postoperative I seed brachytherapy for the treatment of mucoepidermoid carcinoma of the parotid gland in pediatric patients.

Pediatr Blood Cancer 2017 01 31;64(1):57-63. Epub 2016 Aug 31.

Capital Medical University School of Stomatology, Beijing, P.R. China.

Background: This retrospective study was undertaken to analyze the effectiveness and safety of surgery combined with postoperative I seed brachytherapy in the treatment of mucoepidermoid carcinoma (MEC) of the parotid gland with risk factors in pediatric patients.

Procedure: From September 2002 to January 2012, 24 patients, ages 5-16 years (mean, 13.2 years; median, 12.3 years), with MEC of the parotid gland were included. Patients with high risk factors received I seed brachytherapy (median actuarial D90, 97 Gy) within 4 weeks following surgery. Radioactivity was 18.5-33.3 MBq per seed and the prescription dose was 60-120 Gy. Overall and disease-free survival rates, local control rate, and distant metastasis were recorded. Radiation-associated late side effects, including dermatitis, hearing loss, thyroid nodules, and secondary malignancy, were also evaluated.

Results: During the follow-up period of 5-13.4 years (median, 7.2 years), the overall and disease-free survival rates were all 100%. No patients developed local recurrence, regional/distant metastasis, and no severe radiation-associated complications including the second malignancy were noted.

Conclusion: Surgery combined with postoperative I seed brachytherapy is effective and safe in the treatment of MEC of the parotid gland in pediatric patients, with no evidence of severe late radiation-related complications. More patients and longer follow-up data are still needed to prove the efficacy of I brachytherapy.
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http://dx.doi.org/10.1002/pbc.26217DOI Listing
January 2017

Pulmonary mucormycosis: A case report and review of the literature.

Oncol Lett 2016 May 22;11(5):3049-3053. Epub 2016 Mar 22.

Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.

The current study reports the case of a 15-year-old male who presented to The First Affiliated Hospital of Soochow University (Suzhou, Jiangsu, China) with a 3-day history of anergy and epistaxis. The patient was diagnosed with T-cell acute lymphoblastic leukemia according to the results of a bone marrow examination and received chemotherapy. During the agranulocytosis period, the patient developed pneumonia of the right upper lung (RUL). Once complete remission was achieved, the patient underwent a lobectomy of the RUL, together with amphotericin B therapy, following the confirmation of pulmonary mucormycosis by the histopathological results. The patient experienced 12 months of uneventful follow-up post-surgery.
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http://dx.doi.org/10.3892/ol.2016.4370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841004PMC
May 2016

Comparison of Elastic Properties of Bilateral Carotid Arteries in Relation to Site of Acute Ischemic Stroke Using Velocity Vector Imaging.

Chin Med J (Engl) 2015 Nov;128(21):2960-3

Shandong Provincial Key Laboratory of Diagnosis and Treatment of Cardio-Cerebral Vascular Diseases, Shandong Medical Imaging Research Institute, Shandong University, Jinan, Shandong 250021, China.

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http://dx.doi.org/10.4103/0366-6999.168075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756900PMC
November 2015

Assessment of pancreatic adenocarcinoma: Use of low-dose whole pancreatic CT perfusion and individualized dual-energy CT scanning.

J Med Imaging Radiat Oncol 2015 Oct 29;59(5):590-8. Epub 2015 Jul 29.

Shandong Provincial Medical Imaging Research Institute, Shandong University, Jinan, China.

Introduction: The objective of this study was to investigate the value of low-dose whole pancreatic computed tomography (CT) perfusion integrated with individualized dual-energy CT (DECT) scanning in the diagnosis of pancreatic adenocarcinoma.

Methods: Twenty patients with pancreatic adenocarcinoma underwent pancreatic CT perfusion as well as individualized dual-phase DECT pancreatic scans. Perfusion characteristics of non-tumourous pancreatic parenchyma and pancreatic adenocarcinoma were analysed. Weighted-average 120 kVp images and the optimal monoenergetic images in dual phase were reconstructed and the contrast noise ratio (CNR) of pancreas-to-tumour were compared.

Results: There were significant difference on blood flow as well as blood volume between pancreatic adenocarcinoma and the non-tumourous pancreatic parenchyma (P < 0.05), whereas no difference on permeability (P > 0.05). CNRs of pancreas-to-tumour in individualized pancreatic phase were significantly higher than those in venous phase (P < 0.05), and CNRs of optimal monoenergetic images were higher than those on weighted-average 120 kVp images (P < 0.05) in both phase. Total effective radiation dose of CT examination was around 9.32-13.75 mSv.

Conclusions: Low-dose whole pancreatic CT perfusion can provide functional information, and the individualized pancreatic phase DECT scan is the optimal method for detecting pancreatic adenocarcinomas. The integration of the two techniques has great value in clinical application.
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http://dx.doi.org/10.1111/1754-9485.12342DOI Listing
October 2015

Diagnostic Value of Prospective Electrocardiogram-triggered Dual-source Computed Tomography Angiography for Infants and Children with Interrupted Aortic Arch.

Chin Med J (Engl) 2015 May;128(9):1184-9

Department of CT, Shandong Medical Imaging Research Institute, Shandong University, Jinan, Shandong 250021, China.

Background: Accurate assessment of intra- as well as extra-cardiac malformations and radiation dosage concerns are especially crucial to infants and children with interrupted aortic arch (IAA). The purpose of this study is to investigate the value of prospective electrocardiogram (ECG)-triggered dual-source computed tomography (DSCT) angiography with low-dosage techniques in the diagnosis of IAA.

Methods: Thirteen patients with suspected IAA underwent prospective ECG-triggered DSCT scan and transthoracic echocardiography (TTE). Surgery was performed on all the patients. A five-point scale was used to assess image quality. The diagnostic accuracy of DSCT angiography and TTE was compared with the surgical findings as the reference standard. A nonparametric Chi-square test was used for comparative analysis. P <0.05 was considered as a significant difference. The mean effective radiation dose (ED) was calculated.

Results: Diagnostic DSCT images were obtained for all the patients. Thirteen IAA cases with 60 separate cardiovascular anomalies were confirmed by surgical findings. The diagnostic accuracy of TTE and DSCT for total cardiovascular malformations was 93.7% and 97.9% (P > 0.05), and that for extra-cardiac vascular malformations was 92.3% and 99.0% (P < 0.05), respectively. The mean score of image quality was 3.77 ± 0.83. The mean ED was 0.30 ± 0.04 mSv (range from 0.23 mSv to 0.39 mSv).

Conclusions: In infants and children with IAA, prospective ECG-triggered DSCT with low radiation exposure and high diagnostic efficiency has higher accuracy compared to TTE in detection of extra-cardiac vascular anomalies.
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http://dx.doi.org/10.4103/0366-6999.156109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831545PMC
May 2015

Chest imaging of H7N9 subtype of human avian influenza.

Radiol Infect Dis 2015 Mar 27;1(2):51-56. Epub 2015 Feb 27.

Department of Infectious Diseases, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China.

Background: Human infection with avian influenza A H7N9 virus is an acute respiratory infectious disease, which usually causes severe pneumonia with a high mortality. Chest radiographs and Computed Tomography (CT) are principal radiological modalities to assess the lung abnormalities.

Objectives: The goal of this study was to investigate the chest images characteristic of H7N9 subtype of human avian influenza.

Materials And Methods: The clinical and imaging data of 11 cases diagnosed as H7N9 subtype of human avian influenza were collected from 4 cities in the southern region of the Yangtze River, China. The chest imaging manifestations were analyzed by the assigned expert group. The analyzed cases include 7 males and 4 females aged from 20 to 84 years, with a mean of 55.6 years. The clinical symptoms were mainly fever (100%, 11/11) and cough (72.7%, 8/11).

Results: Segmental or lobar ground-glass opacity (GGO) or consolidation was shown in 8 cases (72.7% or 8/11). Air bronchogram was found in 7 cases (63.6% or 7/11). The lesions developed into multiple or diffuse in both lungs rapidly at the progressive stage. The reticulation shadows were shown after some lesions absorbed at the stable stage.

Conclusions: The characteristic imaging demonstrations of H7N9 subtype of human avian influenza are segmental or lobar exudative lesions at lungs at the initial stage, which rapidly progress into bilateral distribution at lungs at the progressive stage.
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http://dx.doi.org/10.1016/j.jrid.2015.02.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104112PMC
March 2015
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