Publications by authors named "Xi Yuan"

124 Publications

Biochar derived from spent mushroom substrate reduced NO emissions with lower water content but increased CH emissions under flooded condition from fertilized soils in Camellia oleifera plantations.

Chemosphere 2021 Aug 31;287(Pt 1):132110. Epub 2021 Aug 31.

Key Laboratory of Silviculture, College of Forestry, Jiangxi Agricultural University, Nanchang, 330045, China. Electronic address:

Agricultural soils are major sources of greenhouse gases (GHGs) that related with intensive fertilizer input. Biochar is widely used to mitigate GHGs, which may interact with soil water content impacting GHG emissions. Camellia oleifera fruit shell (FS) and spent mushroom substrate (MS) are ideal biochar feedstocks. However, the impact of water content and biochar on soil GHG emissions has not been thoroughly understood. Here, we examined CH and NO emissions from C. oleifera plantation soils as affected by biochar (derived from MS or FS, 1 g 25 g soil), water content (60%, 120%, 240% or 360% water holding capacity, WHC), and fertilization (control or chicken manure, CM 2.5 g 25 g soil). We determined the abundance of related microbial functional genes to obtain the underlining mechanisms. The results showed that higher NO emissions occurred in soils with 120%WHC, due to increased abundance of AOA, AOB and nirS. MS or FS biochar differed in their effects on soil GHG emissions with different WHC. MS biochar was higher in pH, C/N and specific surface area, and mitigated more NO emissions from soils with CM and 120%WHC relative to FS biochar (by 92.9% and 34.6%, respectively). MS biochar significantly decreased abundance of nitrification related functional genes (AOA, AOB) in soils with 120%WHC and CM, which explained the decrease in NO emissions. However, MS biochar increased cumulative CH emissions from flooded soils via increase in mcrA abundance. Thereby, biochar feedstocks should be considered in CH and NO mitigations from soils with different water contents.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132110DOI Listing
August 2021

Distribution, sources and risk assessment of PAHs in soil from the water level fluctuation zone of Xiangxi Bay, Three Gorges Reservoir.

Environ Geochem Health 2021 Aug 8. Epub 2021 Aug 8.

College of Hydraulic and Environmental Engineering, China Three Gorges University, Yichang, 443002, Hubei, China.

Information on PAH distribution in the water level fluctuation zone (WLFZ) of Three Gorges Reservoir is limited. In this study, we investigated PAH distribution and sources and assessed PAH risks, over one annual water level fluctuation cycle (June 2017-June 2018) at four elevations spanning the WLFZ (145 m, 155 m, 165 m and 175 m) at seven locations in the water level fluctuation zone along Xiangxi River. The mean total PAH concentration in June 2018 (953 ng g) was significantly higher than in June 2017 (494 ng g), and the horizontal and vertical distributions of PAHs changed significantly. The changes in distribution patterns provided evidence for the cause of increased PAH levels, which were attributed to construction of the Xiangxi River Bridge. Thus, this study of PAH dynamics in the WLFZ soils of Xiangxi Bay also provided valuable information on the impact of bridge construction on WLFZ soils. The change in PAH levels among stations implicated sediment disturbance resulting from bridge construction as the major contributor to the increased PAH levels. Source characterization, based on the ratios of certain PAHs, indicates that PAHs are mainly from the combustion of petroleum fuels, biomass and coal. These ratios indicated that the proportion of PAHs from fuel combustion increased from 2017 to 2018, implicating the heavy equipment used during bridge construction as another source of the increased PAH levels. The incremental lifetime cancer risk (ILCR) model was used to assess the health risk of the PAHs and the range among all age groups (10-10) indicates a potential health risk. The mean effects range-median quotient (M-ERM-Q) was used to assess the ecological risk of PAHs and the range (0.1-0.5) indicates low to medium risk. The increase in PAH levels from 2017 to 2018 increased the risk to public health and the environment. The results of this investigation provide a reference for ecological restoration of the WLFZ and support development of effective policies for environmental and public health. Further, the results provide information on the impact of bridge construction on WLFZ soils and identify research needed to more fully understand PAH dynamics in WLFZ soils.
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http://dx.doi.org/10.1007/s10653-021-01047-1DOI Listing
August 2021

Thermal and photo stability of all inorganic lead halide perovskite nanocrystals.

Phys Chem Chem Phys 2021 Aug 4;23(32):17113-17128. Epub 2021 Aug 4.

School of Physical Science and Technology, MOE Key Laboratory of New Processing Technology for Non-ferrous Metals and Materials, Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured Materials, Guangxi University, Nanning 530004, China.

Inorganic lead halide perovskite (ILHP) nanocrystals (NCs) show great potential in solid state lighting and next generation display technology due to their excellent optical properties. However, almost all ILHP NCs are still facing the problem of unstable luminescence properties caused by heating and/or UV illumination. Further improving the thermal and photo stability of ILHP NCs has become the most urgent challenge for their practical application. This Perspective review specifically focuses on the thermal and photo stability of ILHP NCs, discusses and analyzes the factors that affect the thermal and photo stability of ILHP NCs from the perspective of surface ligands and structure composition, summarizes the current strategies to improve the thermal and photo stability of ILHP NCs, and presents the key challenges and perspectives on the research for the improvement of thermal and photo stability of ILHP NCs.
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http://dx.doi.org/10.1039/d1cp02119bDOI Listing
August 2021

Association between gene polymorphism and risk of rheumatoid arthritis: a meta-analysis.

Aging (Albany NY) 2021 08 2;13(15):19397-19414. Epub 2021 Aug 2.

Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital, Wuhan University, Wuhan 430000, Hubei, China.

Cytotoxic T lymphocyte-associated protein 4 () gene polymorphisms may be involved in the risk of Rheumatoid arthritis (RA). However, evidence for the association remains controversial. Therefore, we performed a meta-analysis to confirm the relationship between gene polymorphisms and RA. The pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to assess the strength of association. Stratified analysis was conducted by ethnicity. In total, 66 case-control studies including 21681 cases and 23457 controls were obtained. For rs3087243 polymorphism, significant association was detected in Asians ( vs. : OR=0.77, 95%CI=0.65-0.90, =0.001; vs. : OR=0.67, 95%CI=0.48-0.94, =0.02) and Caucasians ( vs. : OR=0.89, 95%CI=0.86-0.93, <0.00001; vs. : OR=0.81, 95%CI=0.75-0.88, <0.00001). For rs231775 polymorphism, significant association was observed in the overall ( vs. : OR =1.16, 95%CI=1.08-1.25, <0.0001; vs. : OR=1.29, 95%CI=1.12-1.50, =0.0006), and in Asians ( vs. : OR=1.27, 95%CI=1.10-1.47, =0.001; vs. : OR=1.58, 95%CI=1.24-2.01, =0.0002), but not in Caucasians. However, there was no association between rs5742909 polymorphism and RA. This meta-analysis confirmed that rs3087243 and rs231775 polymorphism were associated with the risk of RA in both overall population and ethnic-specific analysis, but there was no association between rs5742909 polymorphism and RA risk.
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http://dx.doi.org/10.18632/aging.203349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386564PMC
August 2021

Integrating Genome-Wide Association Analysis With Transcriptome Sequencing to Identify Candidate Genes Related to Blooming Time in .

Front Plant Sci 2021 15;12:690841. Epub 2021 Jul 15.

National Engineering Research Center for Floriculture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, China.

is one of the most important woody perennials for edible and ornamental use. Despite a substantial variation in the flowering phenology among the germplasm resources, the genetic control for flowering time remains to be elucidated. In this study, we examined five blooming time-related traits of 235 landraces for 2 years. Based on the phenotypic data, we performed genome-wide association studies, which included a combination of marker- and gene-based association tests, and identified 1,445 candidate genes that are consistently linked with flowering time across multiple years. Furthermore, we assessed the global transcriptome change of floral buds from the two cultivars exhibiting contrasting bloom dates and detected 617 associated genes that were differentially expressed during the flowering process. By integrating a co-expression network analysis, we screened out 191 gene candidates of conserved transcriptional pattern during blooming across cultivars. Finally, we validated the temporal expression profiles of these candidates and highlighted their putative roles in regulating floral bud break and blooming time in . Our findings are important to expand the understanding of flowering time control in woody perennials and will boost the molecular breeding of novel varieties in .
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http://dx.doi.org/10.3389/fpls.2021.690841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319914PMC
July 2021

Can new energy vehicles help to achieve carbon neutrality targets?

J Environ Manage 2021 Nov 26;297:113348. Epub 2021 Jul 26.

School of Economics, Qingdao University, Qingdao, China. Electronic address:

In the reforms pertaining to the energy structure in the automotive industry, new energy vehicles (NEVs) have long been the focus of government attention, as an effective means to reduce air pollution. Therefore, this paper employs the rolling-window Granger causality test, in order to discuss the environmental benefits of new energy vehicles, so as to explore the active role of the transportation sector in reducing air pollution. By studying the interactions between NEVs and particulate matter (PM) from the time period spanning from 2013:M1 to 2020:M9, we have found that the positive influences from NEVs to PM ascertain that NEVs cannot be considered as an efficient measure to mitigate air pollution. Moreover, these results are not supported by the energy and environment interaction model, which essentially indicates that replacing traditional energy with renewable energy is an effective measure for controlling environmental pollution. In fact, PM tends to have a negative impact on NEVs, which underlines that the air quality index is a leading indicator, particularly when it comes to analyzing the development of the NEVs market. This essentially highlights that in China, NEVs still do not account for a high proportion of car sales, and therefore, its environmental protection effect is not obvious. At the same time, the factor of public awareness regarding environmental protection will thus occupy a considerable proportion in the transmission of NEVs sales. These revelations will help the government to formulate environmental governance policies, and expand the new energy vehicle market to achieve carbon neutrality targets in China.
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http://dx.doi.org/10.1016/j.jenvman.2021.113348DOI Listing
November 2021

Mesenchymal stem cell-derived exosomes: therapeutic implications for rotator cuff injury.

Regen Med 2021 Aug 15;16(8):803-815. Epub 2021 Jul 15.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.

Rotator cuff injuries are a common clinical condition of the shoulder joint. Surgery that involves reattaching the torn tendon to its humeral head bony attachment has a somewhat lower success rate. The scar tissue formed during healing of the rotator cuff leads to poor tendon-related mechanical properties. To promote healing, a range of genetic interventions, as well as cell transplantation, and many other techniques have been explored. In recent years, the therapeutic promise of mesenchymal stem cells (MSCs) has been well documented in animal and clinical studies. Some data have suggested that MSCs can promote angiogenesis, reduce inflammation and cell proliferation and increase collagen deposition. These functions are likely paracrine effects of MSCs, particularly mediated through exosomes. Here, we review the use of MSCs-related exosomes in tissues and organs. We also discuss their potential utility for treating rotator cuff injuries, and explore the underlying mechanisms of their effects.
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http://dx.doi.org/10.2217/rme-2020-0183DOI Listing
August 2021

Near-unity blue-orange dual-emitting Mn-doped perovskite nanocrystals with metal alloying for efficient white light-emitting diodes.

J Colloid Interface Sci 2021 Jul 1;603:864-873. Epub 2021 Jul 1.

College of Physics, Jilin University, Changchun 130023, China. Electronic address:

The tunable dual-color emitting Mn doped CsPbClBr nanocrystals (NCs) with near-unity photoluminescence quantum yield (PL QY) were synthesized through post-treatment of metal bromide at room temperature for fabrication of efficient warm white light-emitting diodes (WLEDs). Especially, the CdBr treated blue-orange emitting Mn doped NCs with various Mn/Pb molar feed ratios exhibit higher PL QY of 97% and longer Mn PL lifetime of 0.9 ms. It is surprisingly found that the X-ray diffraction peak at 31.9° is almost not changed with increasing Br composition, meaning formation of metal alloying due to incorporation of amount of divalent cation in NCs. The strong and stable Mn PL at temperature ranging from 80 K to 360 K are revealed and the temperature-dependent energy transfer efficiencies in Mn doped CsPbClBr NCs are obtained. The enhancement mechanism of Mn PL QY was attributed to improved energy transfer from exciton to Mn d-d transition and suppressed defect state density after post-treatment. The efficient warm WLEDs with color rendering index of 90 and luminous efficacy of 92 lm/W at 10 mA were fabricated by combining blue-orange dual-emitting Mn doped [email protected] and green emissive [email protected] NCs with violet GaN chips.
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http://dx.doi.org/10.1016/j.jcis.2021.06.138DOI Listing
July 2021

Cocultivation Study of spp. and Inspired From Black-Skin-Red-Koji by a Double-Sided Petri Dish.

Front Microbiol 2021 9;12:670684. Epub 2021 Jun 9.

Hubei International Scientific and Technological Cooperation Base of Traditional Fermented Foods, Huazhong Agricultural University, Wuhan, China.

Cocultivation is an emerging and potential way to investigate microbial interaction in the laboratory. Extensive researches has been carried out over the years, but some microorganism cocultivation are not easy to implement in the laboratory, especially the fungus-fungus (FF) cocultivation, owing to the obstacles such as fungal different growth rate, limited growing space, hyphae intertwining, and difficulty of sample separation, etc. In this research, a double-sided petri dish (DSPD) was designed and carried out as a tool to study FF cocultivation in the laboratory. A natural FF cocultivation of spp. and inspired from black-skin-red-koji (BSRK), were studied. By using DSPD, the aforementioned obstacles in the FF cocultivation study were overcome through co-culturing spp. and on each side of DSPD. The characteristics of monocultured and co-cultured spp. and were compared and analyzed, including colonial and microscopic morphologies, and main secondary metabolites (SMs) of spp. analyzed by high performance liquid chromatography. And a novel SM was found to be produced by M7 when co-cultured with CBS 513.88. Since the above mentioned obstacles, were overcome, we obtained good quality of transcriptome data for further analysis. These results indicate that DSPD might be an efficient tool for investigation of microbial interaction, in particular, for FF interaction.
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http://dx.doi.org/10.3389/fmicb.2021.670684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221429PMC
June 2021

Challenges and Opportunities for Clustered Regularly Interspaced Short Palindromic Repeats Based Molecular Biosensing.

ACS Sens 2021 07 18;6(7):2497-2522. Epub 2021 Jun 18.

Department of Mechanical Engineering, Rochester Institute of Technology, Rochester, New York 14623, United States.

Clustered regularly interspaced short palindromic repeats, CRISPR, has recently emerged as a powerful molecular biosensing tool for nucleic acids and other biomarkers due to its unique properties such as collateral cleavage nature, room temperature reaction conditions, and high target-recognition specificity. Numerous platforms have been developed to leverage the CRISPR assay for ultrasensitive biosensing applications. However, to be considered as a new gold standard, several key challenges for CRISPR molecular biosensing must be addressed. In this paper, we briefly review the history of biosensors, followed by the current status of nucleic acid-based detection methods. We then discuss the current challenges pertaining to CRISPR-based nucleic acid detection, followed by the recent breakthroughs addressing these challenges. We focus upon future advancements required to enable rapid, simple, sensitive, specific, multiplexed, amplification-free, and shelf-stable CRISPR-based molecular biosensors.
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http://dx.doi.org/10.1021/acssensors.1c00530DOI Listing
July 2021

Oxygen Vacancy-Driven Reversible Free Radical Catalysis for Environment-Adaptive Cancer Chemodynamic Therapy.

Angew Chem Int Ed Engl 2021 09 24;60(38):20943-20951. Epub 2021 Aug 24.

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China.

Amplifying free radical production by chemical dynamic catalysis to cause oxidative damage to cancer cells has received extensive interest for cancer-specific therapy. The major challenge is inevitable negative modulation on the tumor microenvironment (TME) by these species, hindering durable effectiveness. Here we show for the first time an oxygen vacancy-rich Bi-based regulator that allows environment-adaptive free radical catalysis. Specifically, the regulator catalyzes production of highly toxic O and OH in cancer cells via logic enzymatic reactions yet scavenges accumulation of free radicals and immunosuppressive mediators in TME-associated noncancerous cells. Atomic-level mechanistic studies reveal that such dual-modal regulating behavior is dominated by oxygen vacancies that well fit for free radical catalytic kinetics, along with distinguished cellular fates of this regulator. With this smart regulator, a "two birds with one shot" cancer dynamic therapy can be expected.
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http://dx.doi.org/10.1002/anie.202107556DOI Listing
September 2021

Risk factors for incorrect surgical count during surgery: An observational study.

Int J Nurs Pract 2021 Aug 10;27(4):e12942. Epub 2021 Apr 10.

Department of Surgery, The First Affiliated Hospital of Soochow University, Suzhou City, China.

Background: Incorrect surgical counts are closely related to retained surgical items, which pose a threat to patients. However, the risk factors for incorrect surgical counts have not been identified yet.

Aim: To identify the risk factors associated with incorrect surgical counts during surgery in a tertiary hospital.

Design: An observational case-control study.

Methods: Seventy cases of incorrect surgical counts were reviewed in this study. Data were collected from January 1, 2014, to April 4, 2019. For each case, we included four randomly selected control cases involving the same surgical procedures by the same surgeon within a 6-month period for comparison. The medical data of these cases were extracted for further statistical analysis.

Results: A higher incidence of incorrect surgical counts was observed among surgical counts performed between 8:00 a.m. to 12:00 a.m., emergency operations, prolonged procedures, and/or after addition of surgical items.

Conclusion: Prolonged surgical procedures, emergency operations, time of occurrence, and addition of surgical items were the risk factors related to incorrect surgical counts during surgery.
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http://dx.doi.org/10.1111/ijn.12942DOI Listing
August 2021

Convalescent plasma to treat COVID-19: clinical experience and efficacy.

Aging (Albany NY) 2021 03 18;13(6):7758-7766. Epub 2021 Mar 18.

Hunan Engineering Research Center of Obstetrics and Gynecological Disease, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.

The recent outbreak of COVID-19 in the world is currently a big threat to global health and economy. Convalescent plasma has been confirmed effective against the novel corona virus in preliminary studies. In this paper, we first described the therapeutic schedule, antibody detection method, indications, contraindications of the convalescent plasmas and reported the effectiveness of convalescent plasma therapy by a retrospective cohort study.
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http://dx.doi.org/10.18632/aging.202795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034927PMC
March 2021

Role of thoracic radiotherapy in extensive stage small cell lung cancer: a systemic review and meta-analysis.

Ann Transl Med 2021 Feb;9(4):299

Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Background: The role of thoracic consolidation radiotherapy in patients with extensive stage small cell lung cancer (ES-SCLC) remains controversial. This study aimed to evaluate the efficacy of thoracic radiotherapy (TRT) in these patients.

Methods: A systematic literature search was performed in PubMed, Embase, and the Cochrane library to identify qualified clinical studies. The hazard ratios (HRs) and 95% confidence intervals (CIs) of overall survival (OS), progression-free survival (PFS) and local recurrence-free survival (LRFS) were extracted, and toxicity of the TRT group versus non-TRT group was analyzed.

Results: A total of 12 studies were included in this meta-analysis, including 936 patients in the TRT group and 1,059 patients in the non-TRT group. The combined results showed that TRT significantly improved OS (HR =0.65; 95% CI: 0.55-0.77, P<0.00001), PFS (HR =0.64; 95% CI: 0.56-0.72, P<0.00001) and LRFS (HR =0.38, 95% CI: 0.26-0.53, P<0.00001). Subgroup analysis showed that OS benefits were observed in patients receiving sequential TRT (HR =0.67; 95% CI: 0.54-0.84, P=0.0006). The addition of TRT significantly improved OS in patients over 65 years of age (HR =0.55; 95% CI: 0.40-0.74, P=0.0001). For patients with only one organ metastasis, there was no significant difference in OS between the two groups (HR =0.61; 95% CI: 0.36-1.01, P=0.06). There was no statistical difference in hematologic toxicity (leukopenia, thrombocytopenia, anemia) and non-hematologic toxicity (nausea or vomiting) between the two groups. The incidence of grade ≥3 esophageal toxicity was 4.6% in the TRT group and 0% in the non-TRT group (P=0.0001). Grade ≥3 bronchopulmonary toxicity was 2.9% in the TRT group and 0.8% in the non-TRT group (P=0.02).

Conclusions: TRT improves OS, PFS and LRFS in patients with ES-SCLC, with a low increase in esophageal and bronchopulmonary toxicity. More randomized controlled trials (RCTs) are expected to confirm our conclusions.

Prospero Registration Number: CRD42020190575.
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http://dx.doi.org/10.21037/atm-20-5765DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944300PMC
February 2021

Genetic analysis of rapidly progressing esophageal squamous cell carcinoma: A case report.

Medicine (Baltimore) 2021 Mar;100(9):e24462

Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University, Jiangsu, China.

Introduction: Numerous investigations have been performed to explore candidate biomarker proteins in esophageal squamous cell carcinoma (ESCC) patients, which could predict the response to chemoradiotherapy (CRT). Here we report a patient with unresectable ESCC who had unsatisfactory effects with radiotherapy, chemotherapy and immunotherapy. We performed genetic analysis in this patient to gain insights about the cause of the rapid progression.

Patient Concerns: A 65-year-old man presented with food obstruction, hoarse voice and choking on drinking water for 2 months, and pain behind the breastbone for 1 month.

Diagnosis: The patient was clinically diagnosed with ESCC and staged as T4N1M1 Stage IV.

Interventions: The patient was treated with CRT and immunotherapy. Mutational analyses through high throughput DNA sequencing methodology (next generation sequencing; NGS) was performed on the patient's blood sample.

Outcomes: The tumor progressed rapidly during the treatment period, and the patient passed away only 3 months from the onset of symptoms.

Conclusion: Although the role of TP53 gene and PIK3CA gene in the progression, treatment and sensitivity of esophageal cancer has been studied, the mechanism of their simultaneous appearance has not been demonstrated in relevant studies. We speculate that the reason for the rapid progression in this patient during active treatment might be related to this. Further studies are needed to validate our observations.
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http://dx.doi.org/10.1097/MD.0000000000024462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939201PMC
March 2021

What we know about placenta accreta spectrum (PAS).

Eur J Obstet Gynecol Reprod Biol 2021 Apr 6;259:81-89. Epub 2021 Feb 6.

Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China; China-Canada-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China. Electronic address:

Placenta accreta spectrum (PAS) is an umbrella term for a variety of pregnancy complications due to abnormal placental implantation, including placenta accreta, placenta increta and placenta percreta. During the past several decades, the prevalence of PAS has been increasing, and the clinical importance of this disease is significant because of the severe complications. In this review, we summarized the available evidence-based data for PAS in various aspects: prevalence, risk factors, pathogenesis, clinical presentation and prenatal screening, and clinical management. Meanwhile, we provided a series of prospects in each section for further studies on PAS. Moreover, we first present a visualized workflow for the management of PAS from three steps: predelivery, during delivery and postdelivery.
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http://dx.doi.org/10.1016/j.ejogrb.2021.02.001DOI Listing
April 2021

Preparation and evaluation of ion-exchange porous polyvinyl alcohol microspheres as a potential drug delivery embolization system.

Mater Sci Eng C Mater Biol Appl 2021 Feb 15;121:111889. Epub 2021 Jan 15.

Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address:

The present study aimed to develop a new drug delivery system with efficient drug loading and sustained drug release for potential application in transarterial chemoembolization (TACE). The porous polyvinyl alcohol microspheres (PPVA MS) were prepared by a combination of inverse emulsification and thermal-induced phase separation (TIPS) method, this was followed by the grafting polymerization of sodium 4-styrene sulfonate (SSS) onto the PPVA MS to obtain the grafted PPVA-g-PSSS MS. The prepared PPVA MS showed a well-defined spherical shape with 'honeycomb-like' porous structure, which could be readily tailored by adjusting the quenching temperature. In vitro biocompatibility analysis indicated the non-cytotoxic and hemocompatible nature of PPVA MS. The porous structure and presence of ionically charged groups in the PPVA-g-PSSS MS favoured the loading of cationic doxorubicin (DOX) onto the MS through ionic-interactions and demonstrated a sustained drug release pattern. Moreover, the cytotoxicity of DOX-loaded PPVA-g-PSSS ([email protected]) MS against HepG2 cells and the intracellular uptake of DOX demonstrated the potent in vitro antitumor activity. Furthermore, the central auricular artery embolization in rabbits showed that both the PPVA-g-PSSS and [email protected] MS could occlude the auricular arteries and induced superior embolization effects, such as progressive ear appearance changes, irreversible parenchymal damage and fibrosis, and ultrastructural alternations in endothelial cells. Besides, the DOX fluorescence was distributed around the embolized arteries, without decreasing its intensity when prolonged embolization up to 15 days. These findings suggest that the newly developed [email protected] MS could be employed as a promising drug-loaded embolic agent for the treatment of hepatocellular carcinoma.
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http://dx.doi.org/10.1016/j.msec.2021.111889DOI Listing
February 2021

Decoding the Complex Free Radical Cascade by Using a DNA Framework-Based Artificial DNA Encoder.

Angew Chem Int Ed Engl 2021 05 1;60(19):10745-10755. Epub 2021 Apr 1.

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China.

DNA-based molecular communications (DMC) are critical for regulating biological networks to maintain stable organismic functions. However, the complicated, time-consuming information transmission process involved in genome-coded DMC and the limited, vulnerable decoding activity generally lead to communication impairment or failure, in response to external stimuli. Herein, we present a conceptually innovative DMC strategy mediated by the DNA framework-based artificial DNA encoder. With the free-radical cascade as a proof-of-concept study, the artificial DNA encoder shows active sensing and real-time actuation, in situ and broad free radical-decoding efficacy, as well as robust resistance to environmental noise. It can also block undesirable short-to-medium-range communications between free radicals and inflammatory networks, leading to a synergistic anti-obesity effect. The artificial DNA encoder-based DMC may be generalized to other communication systems for a variety of applications.
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http://dx.doi.org/10.1002/anie.202014088DOI Listing
May 2021

Effects of neuropeptide Y as a feed additive on stimulating the growth of tilapia (Oreochromis niloticus) fed low fish meal diets.

Peptides 2021 04 2;138:170505. Epub 2021 Feb 2.

State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Province Key Lab Oratory for Aquatic Economic Animals, Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China. Electronic address:

Neuropeptide Y is known to stimulate food intake in fish. In this study, we investigated tilapia NPY (tNPY) both for its effects on the growth of tilapia (Oreochromis niloticus, GIFT) in low fish meal and for its thermal stability. Three diets were formulated containing 0, 3 and 10 % fish meal (NF, LF and HF). From these diets, six experimental diets were prepared by spraying either tNPY solution (0.3 μg/g feed) or distilled water (DW) onto the surface of formulated feeds (NF + DW, NF + tNPY, LF + DW, LF + tNPY, HF + DW and HF + tNPY). Tilapia were fed the six experimental diets for 8 weeks. Fish in the NF + tNPY, LF + tNPY and HF + tNPY groups showed increasing trends in the weight gain rate and specific growth rate compared to its corresponding control group. The feed coefficient of group HF + tNPY was significantly lower than that of the control group. The growth performance of the LF + tNPY approached that of the HF + DW group. The mRNA levels of npy in NF + tNPY were significantly higher than those in NF + DW. A field experiment in which tNPY was sprayed in feeds by the vacuum spray method with doses of 0, 0.2 and 0.4 μg/g feed was performed for three months, and the FBW of tilapia receiving tNPY at 0.2 and 0.4 μg/g feed was higher than that of the control group although not significantly. The bioactivity of tNPY was confirmed by its ability to reduce cAMP levels and activate the ERK1/2 pathway. These results demonstrated that tNPY could promote tilapia growth with oral administration low fish meal diets.
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http://dx.doi.org/10.1016/j.peptides.2021.170505DOI Listing
April 2021

Effects of Various Rice-Based Raw Materials on Enhancement of Volatile Aromatic Compounds in Vinegar.

Molecules 2021 Jan 28;26(3). Epub 2021 Jan 28.

Hubei International Scientific and Technological Cooperation Base of Traditional Fermented Foods, Huazhong Agricultural University, Wuhan 430070, China.

vinegar (MV), during whose brewing process spp. and polished rice (PR) are normally used as the starter and the raw material, respectively, is one of the traditional vinegars in China. In this study, the effects of three raw materials, including PR, unhusked rice (UR), and germinated UR (GR), on MV volatile compounds have been investigated. The results revealed that MV of GR (GMV), and its intermediate wine (GMW), exhibited the highest amount of aroma, not only in the concentrations but also in the varieties of the aromatic compounds mainly contributing to the final fragrance. Especially after three years of aging, the contents of benzaldehyde and furfural in GMV could reach to 13.93% and 0.57%, respectively, both of which can coordinate synergistically on enhancing the aroma. We also found that the filtering efficiency was significantly improved when UR and GR were applied as the raw materials, respectively. Therefore, GR might be more suitable raw materials for MV.
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http://dx.doi.org/10.3390/molecules26030687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866154PMC
January 2021

Onion extract gel is not better than other topical treatments in scar management: A meta-analysis from randomised controlled trails.

Int Wound J 2021 Jun 28;18(3):396-409. Epub 2020 Dec 28.

Department of Plastic Surgery, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University), Chongqing, China.

To evaluate the efficacy and safety of onion extract (OE) gel on scar management, a systematic review was performed by searching Embase, PubMed, Medline, and the Cochrane Library databases, and a meta-analysis was conducted according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement guidelines. Finally, 13 randomised controlled trails were enrolled for meta-analysis. OE gel increased the total improvement scores assessed by investigators (P < .00001) and patients (P < .00001) than no treatment, but no differences were detected between OE gel and other commonly used topical treatments assessed by investigators (P = .56) and patients (P = .39). Moreover, OE in silicone gel increased the total improvement scores assessed by investigators (P < .00001) and patients (P = .0007) than other treatments. OE gel increased the incidence of total adverse effects compared with no treatment (P < .0001) and other treatments (P = .008) by a fixed-effects model, and increased the incidence of dropping out caused by intolerance of treatments (P = .0002). OE gel not only has no superiority to commonly used topical treatments, but also has the potential to increase the incidence of adverse effects on scar management; OE in silicone gel might be the optimal topical choice for scar treatment; however, more evidences are needed to strength these conclusions.
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http://dx.doi.org/10.1111/iwj.13542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244018PMC
June 2021

Feedstock particle size and pyrolysis temperature regulate effects of biochar on soil nitrous oxide and carbon dioxide emissions.

Waste Manag 2021 Feb 3;120:33-40. Epub 2020 Dec 3.

Key Laboratory of Silviculture, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address:

Atmospheric greenhouse gas (GHG) concentration increases are a serious problem impacting global climate. Mitigation of agricultural GHG production is crucial as fertilized soils contribute substantially to changes in GHG atmospheric composition. Biochar derived from agricultural or forestry biowaste has been widely used in agriculture and may help mitigate GHG emissions. While different kinds of biochar and their effects on GHG emissions have been studied, feedstock particle size may interact with pyrolysis temperature to impact biochar effects on GHG emissions, but this has not been investigated. Here, feedstock particle size effects on biochar characteristics and soil nitrous oxide (NO) and carbon dioxide (CO) emissions were studied using Camellia oleifera fruit shell feedstock with three particle size fractions (0.5-2, 2-5, and 5-10 mm) each pyrolyzed at 300, 450, and 600 °C. Results showed that dissolved organic carbon in biochar increased with particle size when pyrolyzed at 300 °C, but decreased with pyrolysis temperature. The 0.5-2 mm shell-derived biochar was associated with the lowest NO and CO emission rates but the highest net nitrogen mineralization rates compared to 2-5 mm and 5-10 mm shell-derived biochar when pyrolyzed at 300 °C. Overall, shell particle size was more important for soil processes at lower pyrolysis temperatures with less variation among particle sizes at higher pyrolysis temperatures. The results indicated that feedstock particle size may interact with pyrolysis temperature and impact mitigation of soil NO and CO emissions.
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http://dx.doi.org/10.1016/j.wasman.2020.11.015DOI Listing
February 2021

Mildly Higher Serum Prolactin Levels Are Directly Proportional to Cumulative Pregnancy Outcomes in Fertilization/Intracytoplasmic Sperm Injection Cycles.

Front Endocrinol (Lausanne) 2020 28;11:584. Epub 2020 Aug 28.

Department of Obstetrics & Gynecology, Peking Union Medical College Hospital (PUMCH), Beijing, China.

Hyperprolactinemia has long been considered detrimental to fertility due to irregularity of ovulation. Whether mild hyperprolactinemia should be corrected before initiating an fertilization/intracytoplasmic sperm injection cycle (IVF/ICSI) has not been determined; this study aimed to examine how different levels of prolactin affect IVF outcomes. A total of 3,009 patients with basal prolactin level <50 ng/mL undergoing IVF/ICSI cycles for tubal or male factors were recruited in this study. Patients diagnosed with anovulation owing to polycystic ovarian syndrome or hyperandrogenism were ruled out. Pregnancy outcomes were compared between patients with basal prolactin levels higher or lower than the median level of prolactin (16.05 ng/mL). Multifactor analyses were carried out among four subgroups depending on different prolactin levels. Repeated-measures analysis of variance was used to explore the relationship between the ascending trend of prolactin levels over ovarian stimulation and the corresponding cumulative pregnancy outcomes. There were significantly higher numbers of oocytes (9 vs. 8, = 0.013) and embryos (6 vs. 5, = 0.015) in patients with basal prolactin higher than 16.05 ng/mL. Basal prolactin higher than 30 ng/mL was positively related to cumulative clinical pregnancy, and a level higher than 40 ng/mL was a good indicator for the cumulative live birth rate. Throughout ovarian stimulation, the prognosis of pregnancy improved with increasing prolactin levels. Patients with better cumulated pregnancy outcomes had significantly higher prolactin levels as well as a profoundly increasing trend during the stimulating process than those who did not conceive. For patients who underwent the gonadotropin-releasing hormone agonist long protocol IVF/ICSI treatment, a slightly higher prolactin level during the controlled ovarian hyperstimulation protocol was a positive indicator for cumulated pregnancy/live birth rates.
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http://dx.doi.org/10.3389/fendo.2020.00584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483656PMC
May 2021

A Cys2/His2 Zinc Finger Protein Acts as a Repressor of the Green Revolution Gene SD1/OsGA20ox2 in Rice (Oryza sativa L.).

Plant Cell Physiol 2021 Feb;61(12):2055-2066

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.

Gibberellins (GAs) play important roles in the regulation of plant growth and development. The green revolution gene SD1 encoding gibberellin 20-oxidase 2 (GA20ox2) has been widely used in modern rice breeding. However, the molecular mechanism of how SD1/OsGA20ox2 expression is regulated remains unclear. Here, we report a Cys2/His2 zinc finger protein ZFP207 acting as a transcriptional repressor of OsGA20ox2. ZFP207 was mainly accumulated in young tissues and more specifically in culm nodes. ZFP207-overexpression (ZFP207OE) plants displayed semidwarfism phenotype and small grains by modulating cell length. RNA interference of ZFP207 caused increased plant height and grain length. The application of exogenous GA3 could rescue the semidwarf phenotype of ZFP207OE rice seedlings. Moreover, ZFP207 repressed the expression of OsGA20ox2 via binding to its promoter region. Taken together, ZFP207 acts as a transcriptional repressor of SD1/OsGA20ox2 and it may play a critical role in plant growth and development in rice through the fine-tuning of GA biosynthesis .
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http://dx.doi.org/10.1093/pcp/pcaa120DOI Listing
February 2021

Organocatalytic asymmetric synthesis of arylindolyl indolin-3-ones with both axial and central chirality.

Chem Commun (Camb) 2020 Oct;56(83):12648-12651

Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China.

An efficient method for chiral phosphoric acid-catalyzed asymmetric synthesis of arylindolyl indolin-3-ones with both axial and central chirality has been developed via the reaction of 3-arylindoles with 2-aryl-3H-indol-3-ones, and the target products were obtained in high yields with excellent enantioselectivity and diastereoselectivity.
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http://dx.doi.org/10.1039/d0cc05432aDOI Listing
October 2020

Molecular identification, tissue distribution and in vitro functional analysis of growth hormone and its receptors in red-spotted grouper (Epinephelus akaara).

Comp Biochem Physiol B Biochem Mol Biol 2020 Dec 8;250:110488. Epub 2020 Aug 8.

State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. Electronic address:

Red-spotted grouper (Epinephelus akaara) is one of the high economic value grouper species, however, the knowledge regarding its growth is limited. In this study, full-length cDNAs of growth hormone (gh) and its receptors (ghr1 and ghr2) were cloned from the pituitary and liver of red-spotted grouper, respectively. Tissue distribution analysis showed that gh mRNA was predominantly expressed in the pituitary. ghr1 mRNA was highly expressed in the liver, muscle, fat and gonad, while ghr2 mRNA expression was ubiquitously high in the peripheral tissues. However, the mRNA expression of both ghr isoforms was relatively low in the central nervous system. Secretory recombinant grouper GH (rgGH) was expressed in yeast Pichia pastoris and verified. HEK293T cells transiently transfected with the GHR isoforms were used to elucidate the receptor-mediated signaling pathways related to growth regulation. rgGH activated rapid phosphorylation of Janus kinase 2, signal transducer and activator of transcription 5 (STAT5) and extracellular signal-regulated protein kinase 1/2 through GHR1, but only STAT5 was phosphorylated via GHR2. rgGH strongly activated STAT5 phosphorylation and significantly stimulated ghr1, ghr2 and insulin-like growth factor (igf1, igf2) mRNA expression in primary cultured hepatocytes. Data showed that the recombinant protein rgGH played effects on igf1/2 mRNA expression via GHR-mediated signaling pathways. Our findings provide essential information about GH and GHRs characteristics in red-spotted grouper.
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http://dx.doi.org/10.1016/j.cbpb.2020.110488DOI Listing
December 2020

Efficient full-color emitting carbon-dot-based composite phosphors by chemical dispersion.

Nanoscale 2020 Aug 21;12(29):15823-15831. Epub 2020 Jul 21.

School of Physics and Electronic Engineering, Mudanjiang Normal University, Mudanjiang 157011, China.

Realizing full-color emission plays a key role in exploring the luminescence mechanisms of carbon dots (CDots) and promoting the applications of CDots in light-emitting diodes (LEDs). Herein, a synthesis strategy for full-color emitting CDots was developed through the solvothermal reaction of citric acid and urea with a constant mass ratio but varying reactant concentrations in solvent. With the reactant concentrations increasing, a dual regulation mechanism including an enhanced nucleation growth process and subsequently increased C[double bond, length as m-dash]O/C[double bond, length as m-dash]N-related surface states should be responsible for the photoluminescence (PL) shift of CDots from blue to red. Relying on the hydrolyzation and condensation processes of 3-aminopropyltrimethoxysilane, a simple and universal method was developed by chemically dispersing the CDots into a cross-linked silica network on the surface of SiO nanoparticles to produce efficient full-color emitting SiO/CDot composite phosphors with considerable PL quantum yields in the range of 30-60%. It was proved that the full-color emitting SiO/CDot composite phosphors could be flexibly applied in packaging white LEDs, releasing pure white light at the Commission Internationale de L'Eclairage (CIE) coordinates of (0.33, 0.33) with a color rendering index (CRI) of 80.4 and a high color-rendering white light coming entirely from CDots with the CIE coordinates of (0.34, 0.36) and a CRI of 97.4, indicating promising application of the full-color emitting SiO/CDot composite phosphors in the LED field.
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http://dx.doi.org/10.1039/d0nr02021dDOI Listing
August 2020

Current and Perspective Diagnostic Techniques for COVID-19.

ACS Infect Dis 2020 08 4;6(8):1998-2016. Epub 2020 Aug 4.

Center of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, Guangdong 518055, China.

Since late December 2019, the coronavirus pandemic (COVID-19; previously known as 2019-nCoV) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been surging rapidly around the world. With more than 1,700,000 confirmed cases, the world faces an unprecedented economic, social, and health impact. The early, rapid, sensitive, and accurate diagnosis of viral infection provides rapid responses for public health surveillance, prevention, and control of contagious diffusion. More than 30% of the confirmed cases are asymptomatic, and the high false-negative rate (FNR) of a single assay requires the development of novel diagnostic techniques, combinative approaches, sampling from different locations, and consecutive detection. The recurrence of discharged patients indicates the need for long-term monitoring and tracking. Diagnostic and therapeutic methods are evolving with a deeper understanding of virus pathology and the potential for relapse. In this Review, a comprehensive summary and comparison of different SARS-CoV-2 diagnostic methods are provided for researchers and clinicians to develop appropriate strategies for the timely and effective detection of SARS-CoV-2. The survey of current biosensors and diagnostic devices for viral nucleic acids, proteins, and particles and chest tomography will provide insight into the development of novel perspective techniques for the diagnosis of COVID-19.
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http://dx.doi.org/10.1021/acsinfecdis.0c00365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409380PMC
August 2020

Successive mineral nitrogen or phosphorus fertilization alone significantly altered bacterial community rather than bacterial biomass in plantation soil.

Appl Microbiol Biotechnol 2020 Aug 7;104(16):7213-7224. Epub 2020 Jul 7.

College of Forestry, Jiangxi Agricultural University, Nanchang, 330045, China.

Bacteria play determining roles in forest soil environment and contribute to essential functions in the cycling of nitrogen (N) and phosphorus (P). Understanding the effects of different fertilizer applications, especially successive fertilization, on soil properties and bacterial community could reveal the impacts of fertilization on forest soil ecology and shed light on the nutrient cycling in forest system. This study aimed to evaluate the impacts of successive mineral N (NHNO) and P (NaHPO) fertilization at different rates, alone or together, on soil bacterial biomass and communities at 0-5, 5-10, and 10-20 cm. Compared with the control, N fertilization decreased soil pH, but P alone or with N fertilization had negligibly negative impacts on soil pH. Different mineral fertilizer applications, alone or together, showed no significant effects on soil organic matter contents, relative to the control treatment. Bacterial biomass remained stable to different fertilizations but decreased with sampling depths. Sole N or P fertilization, rather than combined fertilizations, significantly changed soil bacterial community structures. Our results demonstrated that mineral N or P fertilization alone significantly affected bacterial community structures rather than biomass in the plantation soils. KEY POINTS: • Impacts of successive mineral fertilization on soil bacteria were determined. • Mineral fertilization showed negligible impacts on bacterial biomass. • N additions stimulated Chloroflexi relative abundances. • Mineral N or P fertilization significantly altered bacterial community structure.
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http://dx.doi.org/10.1007/s00253-020-10761-2DOI Listing
August 2020

RAF dimer inhibition enhances the antitumor activity of MEK inhibitors in K-RAS mutant tumors.

Mol Oncol 2020 08 18;14(8):1833-1849. Epub 2020 May 18.

External Innovation, BeiGene, Ltd., San Mateo, CA, USA.

The mutation of K-RAS represents one of the most frequent genetic alterations in cancer. Targeting of downstream effectors of RAS, including of MEK and ERK, has limited clinical success in cancer patients with K-RAS mutations. The reduced sensitivity of K-RAS-mutated cells to certain MEK inhibitors (MEKi) is associated with the feedback phosphorylation of MEK by C-RAF and with the reactivation of mitogen-activated protein kinase (MAPK) signaling. Here, we report that the RAF dimer inhibitors lifirafenib (BGB-283) and compound C show a strong synergistic effect with MEKi, including mirdametinib (PD-0325901) and selumetinib, in suppressing the proliferation of K-RAS-mutated non-small-cell lung cancer and colorectal cancer (CRC) cell lines. This synergistic effect was not observed with the B-RAF selective inhibitor vemurafenib. Our mechanistic analysis revealed that RAF dimer inhibition suppresses RAF-dependent MEK reactivation and leads to the sustained inhibition of MAPK signaling in K-RAS-mutated cells. This synergistic effect was also observed in several K-RAS mutant mouse xenograft models. A pharmacodynamic analysis supported a role for the synergistic phospho-ERK blockade in enhancing the antitumor activity observed in the K-RAS mutant models. These findings support a vertical inhibition strategy in which RAF dimer and MEKi are combined to target K-RAS-mutated cancers, and have led to a Phase 1b/2 combination therapy study of lifirafenib and mirdametinib in solid tumor patients with K-RAS mutations and other MAPK pathway aberrations.
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http://dx.doi.org/10.1002/1878-0261.12698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400788PMC
August 2020
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