Publications by authors named "Xi Yang"

1,507 Publications

  • Page 1 of 1

Safety and Efficacy of Checkpoint Inhibition in Patients With Melanoma and Preexisting Autoimmune Disease.

Ann Intern Med 2021 Sep;174(9):1345

UPMC Hillman Cancer Center, Pittsburgh, Pennsylvania.

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http://dx.doi.org/10.7326/L21-0441DOI Listing
September 2021

Use of metoclopramide in the first trimester and risk of major congenital malformations: A systematic review and meta-analysis.

PLoS One 2021 20;16(9):e0257584. Epub 2021 Sep 20.

Department of Pharmacy, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, Hunan, China.

Background: Nausea and vomiting of pregnancy affects up to 80% of pregnant women, it typically occurs during the first trimester which is the most sensitive time for environmental exposures given organogenesis. Metoclopramide is an antiemetic drug used widely during NVP, but the findings of studies evaluating its safety of use in pregnancy is inconsistent. Therefore, we conducted a systematic review and meta-analysis to assess whether metoclopramide use during first trimester of pregnancy is associated with the risk of major congenital malformations.

Methods: The systematic search using database included Pubmed, Embase, Web of science, and Cochrane library. Studies written in English, comprising with an exposed group and a control group, reporting major congenital malformation as an outcome were included.

Results: Six studies assessing a total number of 33374 metoclopramide-exposed and 373498 controls infants were included in this meta-analysis. No significant increase in the rate of major congenital malformation was detected following metoclopramide use during first trimester (OR, 1.14; 95% CI, 0.93-1.38).

Conclusions: Metoclopramide use during first trimester of pregnancy was not associated with the risk of major congenital malformations.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0257584PLOS
September 2021

Operative management of equinus associated with lower limb venous malformations.

Phlebology 2021 Sep 20:2683555211047286. Epub 2021 Sep 20.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objectives: Functional impairment is a common complaint in patients with venous malformations. Equinus can occur when the venous malformation involves the lower limb, a challenging condition with only a few studies to guide treatment choices. This study was aimed to investigate the operative management of equinus associated with lower limb venous malformations.

Method: Between August 2015 and September 2017, a total of 12 patients presented with equinus associated with lower limb venous malformations and underwent surgical correction. Preoperative and postoperative clinical symptoms, physical examination and orthopaedic evaluation were retrospectively reviewed. 8 patients who experienced pain underwent percutaneous sclerotherapy prior to the operation. Surgical management included gastrocnemius intramuscular aponeurotic recession, Z-lengthening of the Achilles tendon, Hoke technique and Taylor Spatial Frame external fixation.

Results: There were eight female and four male patients with a mean age of 14.3 ± 5.9 years. The mean follow-up period was 34.8 ± 9 months. The range of motion of ankle dorsiflexion (with knee extended) improved for each patient (mean, 25.4 degrees; standard deviation, 8.5 degrees). No neurovascular complications were observed.

Conclusion: Operative correction of equinus associated with lower limb venous malformations is safe and effective. Selective preoperative sclerotherapy is necessary for optimal outcomes.
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http://dx.doi.org/10.1177/02683555211047286DOI Listing
September 2021

CNV Analysis of the Correlation between Preoperative Lymph Node Metastasis and Prognosis of Early Tongue Cancer.

J Cancer 2021 25;12(20):6135-6144. Epub 2021 Aug 25.

Department of Oral & MaxillofacialeHead & Neck Oncology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200011, China.

To investigate the relationship between preoperative concealed lymph node metastasis (LNM) and prognosis in patients with early tongue cancer. According to preoperative lymph node dissection, 41 patients with tongue cancer were divided into the LNM group (=19) and the non-LNM group (=22). Analysis of single nucleotide variation (SNV), tumor mutation burden (TMB), mutant allele tumor heterogeneity (MATH), aneuploidy and copy number variation (CNV) was performed to identify differentially expressed genes (DEGs) related to LNM. While KEGG analysis was conducted to reveal the CNV differentially expressed genes and main enriched pathways, the correlation between pathways and genes was analyzed by hierarchical clustering. The clinical information of LNM and data on overall survival (OS) rate were obtained from The Cancer Genome Atlas (TCGA), and survival analysis was performed based on combined LNM information. We observed significant correlations for the mTOR signaling pathway, Hippo signaling pathway and Wnt signaling pathway with the LNM group, while leukocyte transendothelial migration and cytokine-cytokine receptor interaction were markedly correlated with the non-LNM group. Moreover, TNFRSF10C was identified as the main DEG in the leukocyte transendothelial migration pathway. TCGA-based validation revealed that the disease-free survival (DFS) of the non-LNM group was significantly higher than that of the LNM group (<0.005) when TNFRSF10C CNV was set to a log2 ratio>-0.163. The differential expression of TNFRSF10C in leukocyte transendothelial migration, an immune-related pathway, is associated with LNM and DFS. The TNFRSF10C CNV log2 ratio could potentially serve as an indicator of good prognosis for tongue cancer patients with non-LNM after neck dissection.
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http://dx.doi.org/10.7150/jca.60447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425219PMC
August 2021

Strain-Induced Alternating Photoluminescence Segmentation in Hexagonal Monolayer Tungsten Disulfide Grown by Physical Vapor Deposition.

ACS Appl Mater Interfaces 2021 Sep 17. Epub 2021 Sep 17.

College of Advanced Interdisciplinary Studies & Hunan Provincial Key Laboratory of Novel Nano-Optoelectronic Information Materials and Devices, National University of Defense Technology, Changsha, Hunan 410073, China.

Two-dimensional semiconductors exhibit strong light emission under optical or electrical pumping due to quantum confinement and large exciton binding energies. The regulation of the light emission shows great application potential in next-generation optoelectronic devices. Herein, by the physical vapor deposition strategy, we synthesize monolayer hexagonal-shaped WS, and its photoluminescence intensity mapping show three-fold symmetric patterns with alternating bright and dark regions. Regardless of the length of the edges, all domains with S-terminated edges show lower photoluminescence intensity, while all regions with W-terminated edges exhibit higher photoluminescence intensity. The photoluminescence segmentation mechanism is studied in detail by employing Raman spectroscopy, atomic force microscopy, high-resolution transmission electron microscopy, and Kelvin probe force microscopy, and it is found to originate from different strain distributions in the S-terminated region and the W-terminated region. The optical band gap determined by the photoluminescence in the dark region is ∼2 meV lower than that in the bright region, implying that more strain is stored in the S-terminated region than in the W-terminated region. The photoluminescence segmentation vanishes in transferred hexagonal-shaped WS from the initial substrate to a fresh silicon substrate, further confirming the physical mechanism. Our results provide guidance for tuning the optical properties of two-dimensional semiconductors by controllable strain engineering.
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http://dx.doi.org/10.1021/acsami.1c13096DOI Listing
September 2021

Homogeneously high expression of CD32b makes it a potential target for CAR-T therapy for chronic lymphocytic leukemia.

J Hematol Oncol 2021 Sep 16;14(1):149. Epub 2021 Sep 16.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300020, China.

CD19 chimeric antigen receptor (CAR)-T cells have been used to treat patients with refractory chronic lymphocytic leukemia (CLL). However, approximately 50% of patients do not respond to this therapy. To improve the clinical outcome of these patients, it is necessary to develop strategies with other optimal targets to enable secondary or combinational CAR-T cell therapy. By screening a panel of surface antigens, we found that CD32b (FcγRIIb) was homogeneously expressed at high site density on tumor cells from CLL patients. We then developed a second-generation CAR construct targeting CD32b, and T cells transduced with the CD32 CAR efficiently eliminated the CD32b Raji leukemic cell line in vitro and in a mouse xenograft model. Furthermore, CD32b CAR-T cells showed cytotoxicity against primary human CLL cells that were cultured in vitro or transplanted into immunodeficient mice. The efficacy of CD32b CAR T cells correlated with the CD32b density on CLL cells. CD32b is not significantly expressed by non-B hematopoietic cells. Our study thus identifies CD32b as a potential target of CAR-T cell therapy for CLL, although further modification of the CAR construct with a safety mechanism may be required to minimize off-target toxicity.
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http://dx.doi.org/10.1186/s13045-021-01160-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447616PMC
September 2021

Emerging nanomaterials applied for tackling the COVID-19 cytokine storm.

J Mater Chem B 2021 Sep 16. Epub 2021 Sep 16.

Department of Medical Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu, China.

During the global outbreak of coronavirus disease 2019 (COVID-19), a hyperinflammatory state called the cytokine storm was recognized as a major contributor to multiple organ failure and mortality. However, to date, the diagnosis and treatment of the cytokine storm remain major challenges for the clinical prognosis of COVID-19. In this review, we outline various nanomaterial-based strategies for preventing the COVID-19 cytokine storm. We highlight the contribution of nanomaterials to directly inhibit cytokine release. We then discuss how nanomaterials can be used to deliver anti-inflammatory drugs to calm the cytokine storm. Nanomaterials also play crucial roles in diagnostics. Nanomaterial-based biosensors with improved sensitivity and specificity can be used to detect cytokines. In summary, emerging nanomaterials offer platforms and tools for the detection and treatment of the COVID-19 cytokine storm and future pandemic.
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http://dx.doi.org/10.1039/d1tb01446cDOI Listing
September 2021

Identification and Integrated Analysis of circRNA and miRNA of Radiation-Induced Lung Injury in a Mouse Model.

J Inflamm Res 2021 2;14:4421-4431. Epub 2021 Sep 2.

Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, People's Republic of China.

Background: Radiation-induced lung injury (RILI) is a main threat to patients who received thoracic radiotherapy. Thus, understanding the molecular mechanism of RILI is of great importance. Circular RNAs (circRNAs) have been found to act as a regulator of multiple biological processes, and the circRNA-microRNA (miRNA)-mRNA axis could play an important role in the signaling pathway of many human diseases including radiation injury.

Methods: First, the circRNA and miRNA of RILI in a mouse model were investigated. The mice received 12 Gy of thoracic irradiation, and the irradiated lung tissues at 48 hours after irradiation were analyzed by RNA sequencing (RNA-seq) compared with normal lung tissues. Then, Gene Ontology analysis of the target mRNAs of the significantly differently expressed circRNAs was performed.

Results: In the irradiated group, inflammatory changes in lungs were observed; 21 significantly up-regulated and 33 down-regulated significantly miRNAs were identified (p < 0.05). Among 27 differentially expressed circRNAs, 10 were down-regulated and 17 were up-regulated in the irradiated group [log2 (fold change) > 1 or < -1, p<0.05]. These differentially expressed miRNAs took part in a series of cellular processes, such as positive regulation of alpha-beta T-cell proliferation, interstitial matrix, collagen fibril organization, chemokine receptor activity, cellular defense response, and B-cell receptor signaling pathway. The differentially expressed circRNAs were related to Th1 and Th2 differentiation pathways, and the predicted mRNAs were verified.

Conclusion: This study revealed immune-related molecular pathways play an important role in the early response after radiotherapy. In the future, research on the target mechanism and early intervention of circRNAs with associated miRNAs such as circRNA5229, circRNA544, and circRNA3340, could benefit the treatment of RILI.
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http://dx.doi.org/10.2147/JIR.S322736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422032PMC
September 2021

Epigenetic Therapy Promotes the Ratio of Th1/Th17 Lineage to Reverse Immune Evasion and Treat Leukemia Relapse Post-allogeneic Stem Cell Transplantation in Non-APL AML Patients.

Front Mol Biosci 2020 24;7:595395. Epub 2021 Aug 24.

Sichuan Provincial People's Hospital, Affiliated Hospital of University of Electronic Science and Technology of China, Chengdu, China.

To reverse the early-stage relapse post-hematopoietic stem cell transplantation, we investigated the safety and efficacy of a new epigenetic regimen (chidamide and decitabine plus thymalfasin simultaneously) on acute myeloid leukemia patients (excluding acute promyelocytic leukemia). Twenty-four patients were enrolled in this observational study during April 2015 to May 2018. The most common adverse event was reversible CTCAE grade 2 thrombocytopenia (20/24). Strikingly, all 24 patients had response to this epigenetic regimen accompanied with decreased measurable residual disease. The overall survival rate is 79.2% (19/24), with a relapse-free survival rate of 79.2% (19/24). During this regimen treatment, Th1 cells and CD3+CD4-CD8+T cells increased, and Th17 cells decreased gradually. The status of high Th1 and low Th17 cells was still observed on the 3rd month after discontinuation of this regimen. Interestingly, the significantly elevated ratio of Th1/Th17 seemed to reflect the treatment-related immune effect, which may be a valuable marker to be monitored in the early-relapse stage for evaluating the efficacy and prognosis.
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http://dx.doi.org/10.3389/fmolb.2020.595395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421566PMC
August 2021

Change of Consumption Behaviours in the Pandemic of COVID-19: Examining Residents' Consumption Expenditure and Driving Determinants.

Int J Environ Res Public Health 2021 08 31;18(17). Epub 2021 Aug 31.

School of Medicine, Ningbo University, 818 Fenghua Road, Jiangbei District, Ningbo 315211, China.

This study investigated changes of individuals' consumption behaviours during the COVID-19 pandemic and explored the driving determinants in consumption expenditure in Zhejiang China. Based on the 454 samples of survey data, which were collected in 2020 and 2021, it showed a reduction trend in consumption expenditure during the pandemic. Compared to the consumptions before the pandemic, money spent on housing, food, and beverage did not change too much. However, expenditures on wearing, recreation, and education reduced. Age, family size, and household income were significant to the expenditure changes. Online shopping became an important alternative way for residents during the pandemic and the trend is expected to continue even after the pandemic. Based on the findings, suggestions are summarized as two points. First, the young and single residents are the main group for recovering the consumption for wearing, recreation, education, and public transport. Meanwhile, to improve the satisfactions in online shopping, regulations should be issued by the government in improving the quality of goods and service.
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http://dx.doi.org/10.3390/ijerph18179209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431450PMC
August 2021

Revealing the binding properties between resorcinol and DNA.

Luminescence 2021 Sep 9. Epub 2021 Sep 9.

College of Chemistry, Nanchang University, Nanchang, Jiangxi, China.

Resorcinol (1,3-dihydroxybenzene) is a common coupling agent in permanent hair dyes, which has arrested people's attention for its potential hazard to the human health. However, the action mechanism of resorcinol and human DNA has not been elucidated yet. In this research, the binding property between resorcinol and calf thymus DNA (ct-DNA) was first studied through various spectral and molecular docking techniques. Spectral studies showed that the initial fluorescence quenching of resorcinol against DNA was a static one. The result of ΔH < 0 and ΔS > 0 was produced from thermodynamic experiment data, thus it could be concluded that electrostatic force was the major driving force, while the binding constant K was 1.56×10 M at 298 K. The electrostatic binding network between resorcinol and ct-DNA was established explicitly through competitive substitution analysis and other spectral approaches. The result of FT-IR absorption spectra indicated resorcinol had bound to DNA phosphate skeleton. Molecular docking clearly revealed the binding occurred between hydroxyl groups of resorcinol and phosphorus oxygen bonds (P-O) of the DNA skeleton. These findings may deepen our understanding of the action mechanism between resorcinol and ct-DNA and provide some useful data for the effect of resorcinol on human diseases.
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http://dx.doi.org/10.1002/bio.4140DOI Listing
September 2021

Notch2 suppresses the development of allergic rhinitis by promoting FOXP3 expression and Treg cell differentiation.

Life Sci 2021 Sep 1;284:119922. Epub 2021 Sep 1.

Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, 238 Jie-Fang Road, Wuhan, Hubei 430060, PR China; Institute of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, 238 Jie-Fang Road, Wuhan, Hubei 430060, PR China. Electronic address:

Aims: Notch signaling is closely related to a variety of diseases, but the role of Notch2 in allergic rhinitis (AR) remain unclear. This study was performed to investigate the effects of Notch2 on the differentiation of Treg cells and on the inflammatory response of AR.

Materials And Methods: Peripheral blood (including 101 AR patients and 66 Controls) and nasal mucosa (including 19 AR patients and 17 Controls) were collected to detect the expression levels of Notch2, NICD2 and FOXP3. CD4+ T cells of human origin were selected to detect the effects of Notch2 on the differentiation of Treg cells and FOXP3. An AR mouse model was established, and lentiviruses overexpressing Notch2 were administered. Then, allergic symptoms, OVA-sIgE titers, nasal mucosal inflammation, Th1/Th2/Th17 cytokines and splenic Treg cells were assessed.

Key Findings: Compared with that in the Control group, the expression of Notch2 in the AR group was decreased, and Notch2 expression was negatively correlated with the degree of allergy (P < 0.01). The expression levels of Notch2, NICD2 and FOXP3 were decreased in the nasal mucosa of AR patients. Notch2 can promote the differentiation of human Treg cells in vitro (P < 0.05), and Notch2 can directly promote FOXP3 transcription. Animal experiments showed after the upregulation of Notch2 expression, the allergic inflammatory of mice with AR was reduced, the differentiation of Treg cells was increased, and the imbalance of T cells was reversed (P < 0.05).

Significance: Notch2 promotes the differentiation of Treg cells by upregulating FOXP3 expression, thus significantly inhibiting the inflammatory response of AR.
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http://dx.doi.org/10.1016/j.lfs.2021.119922DOI Listing
September 2021

Recent Trends in Sedentary Behaviors among Chinese Children According to Demographic and Social Characteristics.

Biomed Environ Sci 2021 Aug;34(8):593-605

National Institution for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: This study aims to explore trends in sedentary behavior among Chinese children aged 6-17 years per demographic and social characteristics.

Methods: A total of 4,341 children aged 6-17 years who participated in the from 2004 to 2015 were included. Repeated measures from mixed-effects models were applied to test trends in sedentary behaviors, which were further analyzed after applying stratification according to gender, age, urbanization level, residential area, and region. Random-effects ordinal regression models were used to examine the trends at sedentary levels.

Results: From 2004 to 2015, sedentary time among children aged 6-17 years increased from 23.9 ± 0.6 h/week to 25.7 ± 0.6 h/week ( < 0.001). Increases of 3.1 h/week ( < 0.001), 2.3 h/week ( = 0.012), and 1.3 h/week ( = 0.042) were found among the low-, medium-, and high-urbanization groups respectively and increased by 2.0 h/week ( < 0.001) and 1.3 h/week ( = 0.002) among rural and urban children, respectively. The proportion of Chinese children with average daily screen time below 2 hours declined by 10.2% ( = 0.51, 95% : 0.42-0.63, < 0.001); a decreasing trend was observed in both boys and girls.

Conclusions: Sedentary time among Chinese children aged 6-17 years showed an upward trend from 2004 to 2015, especially among children residing in rural areas and regions with low urbanization levels.
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http://dx.doi.org/10.3967/bes2021.083DOI Listing
August 2021

[Curriculum design and implementation of teaching in experimental genetic engineering blended course under the principle of learning-centered teaching].

Sheng Wu Gong Cheng Xue Bao 2021 Aug;37(8):2956-2966

National Demonstration Center for Experimental Biology Education, Fudan University, Shanghai 200433, China.

Teaching in experiments of biology is important for the cultivation of life science talents. In view of the rapid development of life science and the increasing demand for research-oriented talent training, teaching in experiments of biology should set up a variety of learning outcomes: to train experimental skill, to cultivate students' experimental design and operation abilities, and to improve students' scientific thinking and innovative consciousness. We have carried out an educational reform on experimental genetic engineering blended course. In this paper, we introduced our methods of organizing online materials, the curriculum design of the blended course, the implementation details, and a preliminary analysis of teaching effects. We found that experimental genetic engineering blended course could support students' active learning and a learning-centered teaching model. Moreover, it could facilitate students' achievement of improving experimental skills, cultivating a rigorous scientific attitude, professional research quality and academic innovation ability.
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http://dx.doi.org/10.13345/j.cjb.200603DOI Listing
August 2021

Isolated growth hormone deficiency type IA due to a novel GH1 variant: a case report.

BMC Med Genomics 2021 Sep 2;14(1):210. Epub 2021 Sep 2.

Department of Pediatrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jie Fang Avenue, Hankou, Wuhan, 430030, People's Republic of China.

Background: A case of isolated growth hormone deficiency type IA (IGHD IA) caused by novel compound heterozygous mutation in the GH1 gene was reported in this study, which aimed to provide insights that will benefit future diagnosis and treatment.

Case Presentation: We analyzed and summarized the clinical data and genetic test results from a patient with IGHD admitted in March 2019 to the Department of Pediatrics Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. We described the results from a 1-year-9-months old female, whose chief complaint was "growth retardation for more than one year". Her birth length was 49.0 cm, and her birth weight was 3.05 kg. Suboptimal intake (breastfeeding) jaundice lasted for approximately two months following birth. When evaluated at the age of 1-year-9-months old, the patient's height was 61.0 cm (- 7.24 SD), and her weight was 6.4 kg (- 1.50 SD). The patient's physical characteristics included yellowish hair, large and unclosed anterior fontanelles, raised forehead, and a low and flat nose. The major abnormalities observed from the auxiliary examinations included low GH (< 0.05 μg/l), low IGF-1 (16.99 μg/l), and elevated TSH (6.97 mIU/l). Genetic testing revealed two heterozygous variants: a splicing mutation (NG_011676.1(NM_022560.4): c.10 + 1G>T, inherited from her mother) in intron 1 of the GH1 gene and a deletion that encompassed the same gene (chr17: 61973811-61996255, inherited from her father). After hormone replacement therapy with L-thyroxine and recombinant human GH (rhGH), the patient's thyroid function returned to normal, and her serum IGF-1 level significantly improved, which resulted in an accelerated increase in height.

Conclusion: This study described a case of IGHD caused by novel compound heterozygous mutations in the GH1 gene. This study suggested that closer attention should be directed to genetic testing and diagnosis based on clinical characteristics to avoid misdiagnosis.
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http://dx.doi.org/10.1186/s12920-021-01057-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411534PMC
September 2021

Penicillin disrupts mitochondrial function and induces autophagy in colorectal cancer cell lines.

Oncol Lett 2021 Oct 1;22(4):691. Epub 2021 Aug 1.

Diabetes Research Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.

Colorectal cancer is a common malignant tumor of the gastrointestinal tract. Currently, the main treatment is surgical resection, which can be combined with other treatments. However, treatment efficacy is poor, and colorectal cancer is prone to relapse and metastasis; thus, identifying an effective anti-cancer drug is an urgent requirement. The present study examined the antagonistic effect of penicillin on cultured colorectal cancer cells and the related mechanism. A MTT assay was used to assess the growth of the colorectal cancer cells treated with penicillin and to determine the optimal drug concentration. The wound healing and Transwell invasion assays were performed to investigate the effect of penicillin on the migration and invasion of the colorectal cancer cells. Live cell mitochondrial energy metabolism analysis was performed to detect changes in mitochondrial energy metabolism of the colorectal cancer cells, while western blot analysis was used to measure the expression of cytochrome and autophagy-related protein, LC3. RFP-GFP-LC3 lentivirus was used to detect autophagic flux, and autophagosomes were observed using a transmission electron microscope, while flow cytometry was used to analyze the effect of penicillin on cell cycle progression and apoptosis of the colorectal cancer cells. After penicillin treatment, the growth, migration and invasion ability of the colorectal cancer cells were inhibited. The mitochondrial energy metabolism of the cell was impaired, and the basic respiratory capacity, maximum respiratory capacity, respiratory potential, and ATP production were all reduced. The protein expression levels of the autophagy-related proteins, LC3-II/LC3-I increased in a dose- and time-dependent manner. In addition, autophagy flux and the number of autophagosomes increased, and mitochondrial structural damage was observed. The cell cycle was arrested at the G phase, the number of early apoptotic cells increased and the protein expression level of cleaved caspase-3 increased, while penicillin-induced apoptosis was blocked by the autophagy inhibitor 3-MA. In conclusion, penicillin disrupted mitochondrial function and energy metabolism in the colorectal cancer cells, which resulted in the induction of autophagic apoptosis and ultimately the inhibition of cancer cell growth and metastasis.
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http://dx.doi.org/10.3892/ol.2021.12952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358593PMC
October 2021

Association Analysis Revealed That Genes Are Linked to Plant Growth Related Traits in Multiple Environment.

Front Plant Sci 2021 11;12:641087. Epub 2021 Aug 11.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Abscisic acid (ABA), one of phytohormones, plays an important regulatory role in plant growth and development. ABA receptor (pyrabactin resistance 1-like 4) was previously detected to be involved in plant response to a variety of stresses. overexpression could enhance wheat () drought resistance. In order to further investigate TaPYL4's role in regulating development of other major agronomic traits in wheat, genes of , , and were cloned from wheat, respectively. Polymorphism analysis on sequences revealed that encoding regions of the three genes were highly conserved, without any SNP (single nucleotide polymorphism) presence. However, nine SNPs and four SNPs were identified in the promoter regions of and , respectively. Functional molecular markers were developed based on these polymorphisms, which were then used to scan a natural population of 323 common wheat accessions for correlation analysis between genotype and the target phenotypic traits. Both and markers were significantly correlated with plant growth-related traits under multiple environments (well-watered, drought and heat stress treatments). The additive effects of and were verified by the combinational haplotype (-AB1∼-AB4) effects determined from field data. -acting elements were analyzed in the promoters of and , showing that a TGA-element bound by ARFs (auxin response factors) existed only in -2A-1 of . Gene expression assays indicated that was constitutively expressed in various tissues, with higher expression in -2A-1 genotypes than in -2A-2 materials. Notably, TaARF4 could act as transcription activator in -2A-1 materials, but not in -2A-2 genotypes. Analysis of geographic distribution and temporal frequency of haplotypes indicated that -AB1 was positively selected in wheat breeding in China. Therefore, and could be a valuable target gene in wheat genetic improvement to develop the ideal plant architecture.
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http://dx.doi.org/10.3389/fpls.2021.641087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387097PMC
August 2021

Spectroscopic and in silico insight into the interaction between dicofol and human serum albumin.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Aug 20;264:120277. Epub 2021 Aug 20.

College of Chemistry, Nanchang University, Nanchang, Jiangxi 330031, China. Electronic address:

Dicofol, a broad-spectrum acaricide, has garnered considerable attention because of the potential harm to the environment and various organisms. Herein, this study applied spectroscopic and in silico methods to understand the interaction between human serum albumin (HSA) and dicofol. Fluorescence experiments demonstrated that dicofol formed a stable complex and the binding process occurred in Suldow's site I of HSA. Its binding constant was 2.26 × 10 M at 298 K. Van der Waals forces and hydrogen bond were primarily facilitated the interaction between dicofol and HSA (ΔH < 0, ΔS < 0) according to thermodynamic experiments. Additionally, 3D fluorescence and circular dichroism (CD) spectra revealed a few conformational changes in HSA due to dicofol. Molecular docking analysis indicated that dicofol interacted with Ser192, Gln196, Leu481, Arg218, Leu238, and Phe211 via van der Waals forces and formed a hydrogen bond with His242. Molecular dynamics (MD) simulation showed that Lys195 and Arg218 residues contributed greater energy for forming the HSA-dicofol complex. MD simulation analysis also showed that dicofol can affect the HSA structure with a reduction in α-helix. This research is desired to facilitate a new perspective on the toxicity mechanism of dicofol in the human body.
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http://dx.doi.org/10.1016/j.saa.2021.120277DOI Listing
August 2021

Learning lightweight super-resolution networks with weight pruning.

Neural Netw 2021 Aug 13;144:21-32. Epub 2021 Aug 13.

Chongqing Key Laboratory of Image Cognition, Chongqing University of Posts and Telecommunications, Chongqing 400065, China. Electronic address:

Single image super-resolution (SISR) has achieved significant performance improvements due to the deep convolutional neural networks (CNN). However, the deep learning-based method is computationally intensive and memory demanding, which limit its practical deployment, especially for mobile devices. Focusing on this issue, in this paper, we present a novel approach to compress SR networks by weight pruning. To achieve this goal, firstly, we explore a progressive optimization method to gradually zero out the redundant parameters. Then, we construct a sparse-aware attention module by exploring a pruning-based well-suited attention strategy. Finally, we propose an information multi-slicing network which extracts and integrates multi-scale features at a granular level to acquire a more lightweight and accurate SR network. Extensive experiments reflect the pruning method could reduce the model size without a noticeable drop in performance, making it possible to apply the start-of-the-art SR models in the real-world applications. Furthermore, our proposed pruning versions could achieve better accuracy and visual improvements than state-of-the-art methods.
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http://dx.doi.org/10.1016/j.neunet.2021.08.002DOI Listing
August 2021

Design and Implementation of a Composite Hydrophone of Sound Pressure and Sound Pressure Gradient.

Micromachines (Basel) 2021 Aug 10;12(8). Epub 2021 Aug 10.

Key Laboratory of National Defense Science and Technology for Underwater Acoustic Technology, Harbin Engineering University, Harbin 150001, China.

The bionic cilium MEMS vector hydrophone has the characteristics of low power consumption, small volume, and good low-frequency response. Nevertheless, there exists the problem of left-right ambiguity in the azimuth estimation of a single hydrophone. In order to solve the engineering application problem, a sound-pressure sound-pressure-gradient hydrophone is designed in this paper. The new composite hydrophone consists of two channels. The bionic cilium microstructure is optimized and used as the vector channel, to collect the sound pressure gradient information, and a scalar channel, based on a piezoelectric ceramic tube, is added, to receive the sound pressure information. The theoretical analysis, simulation analysis, and test analysis of the composite hydrophone are carried out, respectively. The test results show that the sensitivities of the hydrophone can reach up to -188 dB (vector channel) and -204 dB (scalar channel). The problem of left-right ambiguity is solved by combining the sound pressure and sound pressure gradient in different ways. This is of great significance in the engineering application of single cilium MEMS hydrophone orientation.
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http://dx.doi.org/10.3390/mi12080939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399468PMC
August 2021

gen. nov., sp. nov., isolated from feces of greater white-fronted geese ().

Int J Syst Evol Microbiol 2021 Aug;71(8)

Jiangxi Province Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, Nanchang 330006, PR China.

Two rod-shaped and Gram-stain-positive bacteria (strains C64 and C62) were isolated in 2020 from faeces of greater white-fronted geese () from Poyang Lake, PR China. Their optimal growth conditions were at 37 °C, pH 7.0 and with 0.5 % (w/v) NaCl. The two isolates showed a highest 16S rRNA gene sequence similarity to DSM 19116 (92.1 %). Phylogenetic/phylogenomic analyses indicated that strains C64 and C62 clustered independently in the vicinity of the genera , and within the family , but could not be classified clearly as members of any of these known genera. The average amino acid identity values between our isolates and available genomes of members of the family were around the genus threshold value (45-65 %). The major cellular fatty acids of the strains were Cω and C. The predominant polar lipids were phosphatidylinositol, phosphatidylglycerol, phosphatidylcholine, diacylglycerol, triacylglycerol and cardiolipin. The amino acid composition of peptidoglycan contained alanine, glutamic acid and glycine. The major respiratory menaquinones were MK-8(H) and MK-9(H). The whole cell sugars included galactose, arabinose and glucose. On the basis of the results of the 16S rRNA gene sequences comparison, whole-genome phylogenomic analysis, phenotypic and chemotaxonomic characteristics, we propose that strains C64 and C62 represent a novel species belonging to a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is C64 (=CGMCC 1.18410=GDMCC 1.1969=KCTC 49511=KACC 22143).
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http://dx.doi.org/10.1099/ijsem.0.004978DOI Listing
August 2021

Transformer-Based Named Entity Recognition for Parsing Clinical Trial Eligibility Criteria.

ACM BCB 2021 Aug;2021

Florida State University.

The rapid adoption of electronic health records (EHRs) systems has made clinical data available in electronic format for research and for many downstream applications. Electronic screening of potentially eligible patients using these clinical databases for clinical trials is a critical need to improve trial recruitment efficiency. Nevertheless, manually translating free-text eligibility criteria into database queries is labor intensive and inefficient. To facilitate automated screening, free-text eligibility criteria must be structured and coded into a computable format using controlled vocabularies. Named entity recognition (NER) is thus an important first step. In this study, we evaluate 4 state-of-the-art transformer-based NER models on two publicly available annotated corpora of eligibility criteria released by Columbia University (i.e., the Chia data) and Facebook Research (i.e.the FRD data). Four transformer-based models (i.e., BERT, ALBERT, RoBERTa, and ELECTRA) pretrained with general English domain corpora vs. those pretrained with PubMed citations, clinical notes from the MIMIC-III dataset and eligibility criteria extracted from all the clinical trials on ClinicalTrials.gov were compared. Experimental results show that RoBERTa pretrained with MIMIC-III clinical notes and eligibility criteria yielded the highest strict and relaxed F-scores in both the Chia data (i.e., 0.658/0.798) and the FRD data (i.e., 0.785/0.916). With promising NER results, further investigations on building a reliable natural language processing (NLP)-assisted pipeline for automated electronic screening are needed.
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http://dx.doi.org/10.1145/3459930.3469560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373041PMC
August 2021

Using single-cell sequencing technology to detect circulating tumor cells in solid tumors.

Mol Cancer 2021 08 19;20(1):104. Epub 2021 Aug 19.

Department of Oncology, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, No.1558, Sanhuan North Road, Wuxing District Zhejiang Province, Huzhou, China.

Circulating tumor cells are tumor cells with high vitality and high metastatic potential that invade and shed into the peripheral blood from primary solid tumors or metastatic foci. Due to the heterogeneity of tumors, it is difficult for high-throughput sequencing analysis of tumor tissues to find the genomic characteristics of low-abundance tumor stem cells. Single-cell sequencing of circulating tumor cells avoids interference from tumor heterogeneity by comparing the differences between single-cell genomes, transcriptomes, and epigenetic groups among circulating tumor cells, primary and metastatic tumors, and metastatic lymph nodes in patients' peripheral blood, providing a new perspective for understanding the biological process of tumors. This article describes the identification, biological characteristics, and single-cell genome-wide variation in circulating tumor cells and summarizes the application of single-cell sequencing technology to tumor typing, metastasis analysis, progression detection, and adjuvant therapy.
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http://dx.doi.org/10.1186/s12943-021-01392-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375060PMC
August 2021

A pan-cancer study of spalt-like transcription factors 1/2/3/4 as therapeutic targets.

Arch Biochem Biophys 2021 Oct 17;711:109016. Epub 2021 Aug 17.

The Affiliated Hospital of Medical School, Ningbo University, Ningbo, China; Institute of Biochemistry and Molecular Biology, School of Medicine, Ningbo University, Ningbo, China. Electronic address:

Spalt-like transcription factors (SALLs) are evolutionarily conserved proteins that participate in embryonic development. Four members of the SALL family, SALL1, SALL2, SALL3, and SALL4, are involved in cellular apoptosis, angiogenesis, invasion, and metastasis of tumors. We used the TCGA pan-cancer data to conduct a comprehensive analysis of SALL genes. High heterogeneity in the expression of these genes was observed across various cancers, SALL1 and SALL2 were downregulated, whereas SALL4 was upregulated. Moreover, we verified that SALL4 was commonly associated with survival disadvantage, whereas others were linked to a better prognosis. In renal cancer, SALL1, SALL2, and SALL3 showed downregulation, suggesting that they acted as tumor suppressors. Furthermore, SALLs were associated with immune infiltrate subtypes, with a close association between different degrees of infiltration of stromal cells and immune cells. DNA and RNA analyses in different tumors suggested different degrees of negative or positive correlation with tumor stem cell-like features. Finally, we revealed that SALLs were related to cancer cell resistance. Our results highlight the necessity to further study each SALL gene as a separate entity in specific types of cancer. Although this article showed that SALLs could be promising targets for cancer therapy, it needs further studies to validate the findings.
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http://dx.doi.org/10.1016/j.abb.2021.109016DOI Listing
October 2021

Photoacoustic imaging for monitoring of stroke diseases: A review.

Photoacoustics 2021 Sep 24;23:100287. Epub 2021 Jul 24.

CenBRAIN Lab., School of Engineering, Westlake University, Hangzhou, 310024, Zhejiang, China.

Stroke is the leading cause of death and disability after ischemic heart disease. However, there is lacking a non-invasive long-time monitoring technique for stroke diagnosis and therapy. The photoacoustic imaging approach reconstructs images of an object based on the energy excitation by optical absorption and its conversion to acoustic waves, due to corresponding thermoelastic expansion, which has optical resolution and acoustic propagation. This emerging functional imaging method is a non-invasive technique. Due to its precision, this method is particularly attractive for stroke monitoring purpose. In this paper, we review the achievements of this technology and its applications on stroke, as well as the development status in both animal and human applications. Also, various photoacoustic systems and multi-modality photoacoustic imaging are introduced as for potential clinical applications. Finally, the challenges of photoacoustic imaging for monitoring stroke are discussed.
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http://dx.doi.org/10.1016/j.pacs.2021.100287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353507PMC
September 2021

Development and Comparison of Intramuscularly Injected Long-acting Testosterone Undecanoate Nano-/Microcrystal Suspensions with Three Different Particle Size.

Iran J Pharm Res 2021 ;20(1):307-317

State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.

The aim of this study was to develop and compare the pharmacokinetic property of testosterone undecanoate (TU) nano-/microcrystal suspension with three different particle sizes after intramuscular (i.m.) administration. TU nano-/microcrystal suspensions were prepared by high pressure homogenization method and the mean particle size was 0.30 ± 0.11 μm (A), 1.21 ± 0.37 μm (B), and 4.83 ± 0.60 μm (C), respectively. Scanning electron microscope (SEM) was employed to observe the morphology of nano-/microcrystal suspensions after operation. X-ray Powder diffraction (XRPD) confirmed the crystalline state of TU in nano-/microcrystal suspension. After storage at 4 °C and 25 °C under mechanical shaking for 2 months, physical and chemical stabilities of nano-/microcrystal suspensions were measured by particle size analyzer and high performance liquid chromatography. There was no obvious change in particle size distribution and content of TU. After i.m. administration of suspension C to rats, the concentration of TU in plasma lasted for nearly 12 days that was comparative with the commercial testosterone undecanoate injection. The results showed that microcrystal C with a larger particle size had long-acting effect comparing with other two suspensions. The muscle irritation test in rabbits showed that the local irritation of TU nano-/microcrystal suspensions was lower than that of commercial testosterone undecanoate injection. It can be concluded that appropriate particle size of nano-/microcrystal suspensions for i.m. administration of TU was important to achieve better therapeutic effect.
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http://dx.doi.org/10.22037/ijpr.2019.14408.12370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170747PMC
January 2021

Homologous overexpression of genes in Cordyceps militaris improves the production of polysaccharides.

Food Res Int 2021 09 29;147:110452. Epub 2021 May 29.

School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, People's Republic of China. Electronic address:

The maximum yield of EPS produced by mutant CM-pgm-H was 4.63 ± 0.23 g/L, whereas the yield of wild-type strain was 3.43 ± 0.26 g/L. In addition, the data obtained in the present study indicated that the yield of EPS produced by the engineered strain treated with the co-overexpression of phosphoglucomutase and UDP-glucose 6-dehydrogenase genes achieved 6.11 ± 0.21 g/L, which was increased by 78.13% compared with that by the wild-type strain. CM-pgm-H obtained the highest EPS content than that of gk, ugp, and ugdh mutants. This result indicated that the content of protein phosphoglucomutase was an important influencing factor on the CP production of C. militaris. Furthermore, the EPS production of CM-ugdh-pgm-M was significantly improved by 1.78-fold by co-overexpression. Therefore, our engineering strategies will play an important role in the development of C. militaris for the sustainable production of Cordyceps polysaccharides.
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http://dx.doi.org/10.1016/j.foodres.2021.110452DOI Listing
September 2021

Mechanisms and Implications of CDK4/6 Inhibitors for the Treatment of NSCLC.

Front Oncol 2021 30;11:676041. Epub 2021 Jul 30.

Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.

Cyclin-dependent kinases (CDKs) are key regulators of cell cycle progression in malignant tumor cells and play an important role through complex molecular interactions. Dysregulation of CDK dependent pathways is often found in non-small cell lung cancer, which indicates its vulnerability and can be used in clinical benefit. CDK4/6 inhibitors can prevent tumor cells from entering the G approved 1 and S phases, which have been studied in a series of explorations and brought great clinical effect to patients and encouragement to both physicians and researchers, thereby showing potential as a new therapeutic agent. A series of preclinical and clinical studies have been carried out on CDK4/6 inhibitors in NSCLC, and have been achieved some results, which may become a new potential treatment in the future. This review focuses on the research progress on CDK4/6 inhibitors in NSCLC, particularly the mechanisms of action, drugs, clinical research progress, and future application.
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http://dx.doi.org/10.3389/fonc.2021.676041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361448PMC
July 2021

The characteristics and prospects of reflectance confocal microscopy for noninvasive diagnosis of oral potentially malignant disorders.

Oral Oncol 2021 Oct 12;121:105479. Epub 2021 Aug 12.

Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/j.oraloncology.2021.105479DOI Listing
October 2021
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