Publications by authors named "Xi Bai"

69 Publications

Effect of phloretin on growth performance, serum biochemical parameters and antioxidant profile in heat-stressed broilers.

Poult Sci 2021 Aug 24;100(8):101217. Epub 2021 Apr 24.

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address:

The objective of this work was to evaluate the effect of phloretin on growth performance, serum biochemical parameters, antioxidant profile, glutathione (GSH)-related enzymes, nuclear factor erythroid 2-related 2 (Nrf2) and heat shock protein 70 (HSP70) in heat-stressed broilers. A total of 240, 22-day-old Arbor Acres broilers were divided into 4 groups. The control group was housed at 23.0 ± 0.61°C and fed with basal diet, while the 3 heat-stressed groups (A, B, and C groups) were housed at 30.5 ± 0.69°C and fed with basal diet containing 0, 100, and 200 mg/kg phloretin, respectively. Serum was taken form 42-day-old broilers. Results showed that heat stress decreased (P < 0.05) the final body weight (FBW), body weight gain (BWG), feed intake (FI), serum total protein (TP), triglyceride (TG), triiodothyronine (T3), thyroxine (T4), GSH, catalase (CAT), and total antioxidant capacity (T-AOC) levels, but increased (P < 0.05) the feed-to-gain ratio (FGR) and serum malondialdehyde (MDA) levels in broilers compared with that in the control group. Among the heat-stressed groups, supplementary 200 mg/kg phloretin increased (P < 0.05) the FBW, BWG, FI, serum TP, TG, T4, GSH, CAT, and T-AOC levels, and decreased (P < 0.05) the FGR and serum MDA in broilers. There were significant decreases (P < 0.05) in the glutathione peroxidase (GSH-Px), γ-glutamylcysteine synthetase (γ-GCS), and Nrf2, but significant increases (P < 0.05) in the HSP70 of the broiler serum after heat stress treatment. Among the heat-stressed groups, supplementary 200 mg/kg phloretin increased (P < 0.05) the GSH-Px, γ-GCS, and Nrf2 levels, but decreased (P < 0.05) the serum HSP70 level in the heat-stressed broilers. Under high temperature condition, FBW, BWG, FI, FGR, serum TP, TG, T4, MDA, GSH, CAT, T-AOC, GSH-Px, γ-GCS, Nrf2 and HSP70 were linearly affected by inclusion of phloretin. These results indicated that phloretin may improve growth performance, serum parameters, and antioxidant profiles through regulated GSH-related enzymes, Nrf2 and HSP70 in heat-stressed broilers.
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http://dx.doi.org/10.1016/j.psj.2021.101217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237358PMC
August 2021

Treatment of Short Stature with Aromatase Inhibitors: A Systematic Review and Meta-Analysis.

Horm Metab Res 2021 Jun 21;53(6):391-401. Epub 2021 Jun 21.

Department of Endocrinology, Hebei General Hospital, Shijiazhuang, Hebei, China.

The objective of the study is to determine the risks and benefits of treating idiopathic short stature (ISS) with aromatase inhibitors (AIs). We comprehensively searched PubMed, Embase, and the China National Knowledge Infrastructure between establishment year and January 31, 2020. Mean difference (MD)/Standardized mean differences (SMD) with 95% confidence intervals (CI) of individual studies were pooled using fixed or random effects models. Subgroup and sensitivity analyses were also performed. Publication bias was estimated using funnel plots and Egger tests. Fourteen studies including 388 participants were included. The meta-analysis results showed that AIs significantly increased final height (MD=2.46, 95% CI: 0.8-4.12) and predicted adult height (MD=0.34, 95% CI: 0.11-0.57). Changes in bone age (MD=-0.1, 95% CI: -0.86-0.66) and bone mineral density (MD=-0.05, 95% CI: -0.19-0.1) were not different between intervention and control group. AI significantly increased testosterone level (SMD=2.01, 95% CI: 0.8-3.23) and reduced estradiol level (SMD=-1.13, 95% CI: -1.87 to -0.40); The intervention and control group had no significant differences in the levels of high-density lipoprotein-cholesterol (SMD=-0.31, 95%CI: -0.68-0.06) and IGF-1 (SMD=0.7, 95% CI: -0.66-2.06) levels. Adverse events were more frequent in the intervention group than in the control group (odds ratio=3.12, 95% CI: 1.44-6.73). In conclusion, both AI monotherapy and AI combination therapy can increase predicted adult height and testosterone levels.
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http://dx.doi.org/10.1055/a-1492-2841DOI Listing
June 2021

Spectrum-effect relationship between GC-QTOF-MS fingerprint and antioxidant, anti-inflammatory activities of Schizonepeta tenuifolia essential oil.

Biomed Chromatogr 2021 Jul 12;35(7):e5106. Epub 2021 Mar 12.

State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, China.

Schizonepeta tenuifolia (Benth.) Briq, a traditional Chinese medicine, is an annual herbaceous plant that is widely distributed in China, Japan, and Korea. The essential oil (EO) of S. tenuifolia has antioxidant and anti-inflammatory properties. However, the components contributing to its antioxidant and anti-inflammatory activities remain unclear. This study was aimed at investigating the spectrum-effect relationship between GC-MS fingerprint and the antioxidant and anti-inflammatory effects of S. tenuifolia EO. Here, the fingerprints of EO from 10 batches of S. tenuifolia from various sources were established using GC-MS, and the antioxidant and anti-inflammatory bioactivities were evaluated using 2,2-diphenyl-1-picrylhydrazyl and nitric oxide inhibitory assays, respectively. Finally, 13 common peaks were identified from 10 batches of S. tenuifolia by searching against the standard mass spectra in NIST 14 and comparing the literature retention index. The different sources of S. tenuifolia EO exhibit mild antioxidant activities and significant anti-inflammatory effects. In particular, menthone (peak 3), isomenthone (peak 4), pulegone (peak 7), piperitone (peak 8), and β-caryophyllene (peak 11) might be the dominant constituents responsible for the antioxidant and anti-inflammatory activities of S. tenuifolia EO. This method may provide a time-saving, convenient way to screen the potential effective components of S. tenuifolia EO.
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http://dx.doi.org/10.1002/bmc.5106DOI Listing
July 2021

Distinction of High- and Low-Frequency Repetitive Transcranial Magnetic Stimulation on the Functional Reorganization of the Motor Network in Stroke Patients.

Neural Plast 2021 20;2021:8873221. Epub 2021 Jan 20.

Department of Radiology, Institute of Rehabilitation and Imaging of Brain Function, The Second Clinical Medical College of North Sichuan Medical College, Nanchong Central Hospital, Nanchong, Sichuan, China 637000.

Objective: To investigate the functional reorganization of the motor network after repetitive transcranial magnetic stimulation (rTMS) in stroke patients with motor dysfunction and the distinction between high-frequency rTMS (HF-rTMS) and low-frequency rTMS (LF-rTMS).

Methods: Thirty-three subcortical stroke patients were enrolled and assigned to the HF-rTMS group, LF-rTMS group, and sham group. Each patient of rTMS groups received either 10.0 Hz rTMS over the ipsilesional primary motor cortex (M1) or 1.0 Hz rTMS over the contralesional M1 for 10 consecutive days. A resting-state functional magnetic resonance imaging (fMRI) scan and neurological examinations were performed at baseline and after rTMS. The motor network and functional connectivities intramotor network with the core brain regions including the bilateral M1, premotor area (PMA), and supplementary motor area (SMA) were calculated. Comparisons of functional connectivities and Pearson correlation analysis between functional connectivity changes and behavioral improvement were calculated.

Results: Significant motor improvement was found after rTMS in all groups which was larger in two rTMS groups than in the sham group. The functional connectivities of the motor network were significantly increased in bilateral M1, SMA, and contralesional PMA after real rTMS. These changes were only detected in the regions of the ipsilesional hemisphere in the HF-rTMS group and in the regions of the contralesional hemisphere in the LF-rTMS group. Significantly changed functional connectivities of the intramotor network were found between the ipsilesional M1 and SMA and contralesional PMA, between contralesional M1 and contralesional SMA, between contralesional SMA and ipsilesional SMA and contralesional PMA in the HF-rTMS group in which the changed connectivity between ipsilesional M1 and contralesional PMA was obviously correlated with the motor improvement. In addition, the functional connectivity of the intramotor network between ipsilesional M1 and contralesional PMA was significantly higher in the HF-rTMS group than in the LF-rTMS group.

Conclusion: Both HF-rTMS and LF-rTMS have a positive effect on motor recovery in patients with subcortical stroke and could promote the reorganization of the motor network. HF-rTMS may contribute more to the functional connectivity reorganization of the ipsilesional motor network and realize greater benefit to the motor recovery.
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http://dx.doi.org/10.1155/2021/8873221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840259PMC
January 2021

Morphological Abnormalities in the Basal Ganglia of Dystonia Patients.

Stereotact Funct Neurosurg 2021 20;99(4):351-362. Epub 2021 Jan 20.

Department of Neurosurgery, Charité University Clinic, Berlin, Germany,

Objective: The pathophysiology of dystonia is poorly understood. As opposed to secondary forms of dystonia, primary dystonia has long been believed to lack any neuroanatomical substrate. During trajectory planning for DBS, however, conspicuous T2-hyperinstensive signal alterations (SA) were registered within the target region, even in young patients, where ischemia is rare.

Methods: Fifty MRIs of primary dystonia patients scheduled for DBS were analyzed. Total basal ganglia (BG) volumes, as well as proportionate SA volumes, were measured and compared to 50 age-matched control patients.

Results: There was a 10-fold preponderance of percentaged SA within the globus pallidus (GP) in dystonia patients. The greatest disparity was in young patients <25 years. Also, total BG volume differences were observed with larger GP and markedly smaller putamen and caudate in the dystonia group.

Conclusions: BG morphology in primary dystonia differed from a control population. Volume reductions of the putamen and caudate may reflect functional degeneration, while volume increases of the GP may indicate overactivity. T2-hyperintensive SA in the GP of young primary dystonia patients, where microvascular lesions are highly unlikely, are striking. Their pathogenic role remains unclear.
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http://dx.doi.org/10.1159/000512599DOI Listing
January 2021

Effects of growth hormone on cognitive, motor, and behavioral development in Prader-Willi syndrome children: a meta-analysis of randomized controlled trials.

Endocrine 2021 02 22;71(2):321-330. Epub 2020 Nov 22.

Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Research Center for Behavior Medicine in Growth and Development, Beijing, China.

Purpose: The benefits of growth hormone (GH) therapy in Prader-Willi syndrome (PWS) children are well established, but there is still considerable controversy regarding whether GH treatment can improve cognitive, motor, and behavioral development in PWS children. The objectives of this meta-analysis were to quantitatively evaluate the effects of GH on cognitive, motor function, and behavioral development in PWS children.

Methods: Randomized controlled trials (RCTs) examining the effects of GH on cognitive, motor, and behavioral development in PWS children were identified by searching the MEDLINE, EMBASE, and Cochrane Library databases. Intervention effects were represented by Hedges'g and pooled to calculate effect sizes using a random-effects model.

Results: Ten relevant studies comprising data from 302 participants were finally included. We observed no significant difference in cognitive performance between the GH treatment group and the control group (p = 0.197). GH treatment was shown to remarkably improve motor development in PWS children compared with the control treatment (p < 0.001), with moderate positive treatment effects (Hedges'g [95% CI] = 0.71 [0.38, 1.03]). There were no significant differences between the GH group and the control group based on objective assessments of behavioral development (p = 0.53).

Conclusions: The meta-analysis suggested that GH treatment had a significantly positive effect on motor development, with moderate treatment effects in PWS children; however, there was no evidence of effects on cognitive or behavioral development.
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http://dx.doi.org/10.1007/s12020-020-02547-3DOI Listing
February 2021

The Effects of Deep Brain Stimulation of the Subthalamic Nucleus on Vascular Endothelial Growth Factor, Brain-Derived Neurotrophic Factor, and Glial Cell Line-Derived Neurotrophic Factor in a Rat Model of Parkinson's Disease.

Stereotact Funct Neurosurg 2021 5;99(3):256-266. Epub 2020 Nov 5.

Beelitz Neurology, Rehabilitation Clinic, Berlin, Germany.

Objective: Deep brain stimulation (DBS) of the subthalamic nucleus (STN) has evolved as a powerful therapeutic alternative for the treatment of Parkinson's disease (PD). Despite its clinical efficacy, the mechanisms of action have remained poorly understood. In addition to the immediate symptomatic effects, long-term neuroprotective effects have been suggested. Those may be mediated through neurotrophic factors (NFs) like vascular endothelial growth factor (VEGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neurotrophic factor (GDNF). Here, the impact of DBS on the expression of NFs was analysed in a rat model of PD.

Methods: Unilateral 6-hydroxydopamine (6-OHDA) lesioned rats received DBS in the STN using an implantable microstimulation system, sham DBS in the STN, or no electrode placement. Continuous unilateral STN-DBS (current intensity 50 µA, frequency 130 Hz, and pulse width 52 µs) was conducted for 14 days. Rats were then sacrificed and brains shock frozen. Striata and motor cortices were dissected with a cryostat. Levels of VEGF, BDNF, and GDNF were analysed, both by quantitative PCR and colorimetric ELISA.

Results: PCR revealed a significant upregulation of only BDNF mRNA in the ipsilateral striata of the DBS group, when compared to the sham-stimulated group. There was no significant increase in VEGF mRNA or GDNF mRNA. ELISA analysis showed augmentations of BDNF, VEGF, as well as GDNF protein in the ipsilateral striata after DBS compared to sham stimulation. In the motor cortex, significant increases after DBS were observed for BDNF only, not for the other 2 NFs.

Conclusions: The upregulation of trophic factors induced by STN-DBS may participate in its long-term therapeutic efficacy and potentially neuroprotective effects.
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http://dx.doi.org/10.1159/000511121DOI Listing
November 2020

The association of diabetes and the prognosis of COVID-19 patients: A retrospective study.

Diabetes Res Clin Pract 2020 Nov 25;169:108386. Epub 2020 Aug 25.

Division of Endocrinology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Ave., Wuhan 430030, China. Electronic address:

Aims: This study evaluated the impact of previous glycemic control and in-hospital use of antidiabetic/antihypertensive drugs on the prognosis of COVID-19 patients with diabetes.

Methods: In this retrospective cohort study, consecutive inpatients with laboratory confirmed COVID-19 were enrolled from Tongji Hospital (Wuhan, China). Patients without diabetes were matched to those with diabetes based on age, sex, and comorbidities. All patients were followed up to a clinical endpoint (discharge, worsening including transferring to ICU or immediate death). Data and outcomes were extracted from medical records and analyzed.

Results: 64 patients with pre-existing diabetes were included in this study, with 128 matched patients without diabetes included as a control group. Patients with diabetes had a higher rate of worsening (18.8% versus 7.8%, p = 0.025). Multivariable regression showed increased odds of worsening associated with previous glycemic control reflected by HbA1c (odds ratio 3.29, 95% CI 1.19-9.13, p = 0.022) and receiver-operating characteristics (ROC) curve identified HbA1c of 8.6% (70 mmol/mol) as the optimal cut-off value. Univariate analysis demonstrated the in-hospital use of antidiabetic/antihypertensive drugs were not associated with a higher risk of worsening.

Conclusions: COVID-19 patients with diabetes had a higher risk of worsening, especially those with poorly-controlled HbA1c, with an optimal cut-off value of 8.6%. The in-hospital use of antidiabetic/antihypertensive drugs were not associated with increased odds of worsening in patients with diabetes.
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http://dx.doi.org/10.1016/j.diabres.2020.108386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445120PMC
November 2020

Phosphatase AtDBP1 negatively regulates drought and salt tolerance through altering leaf surface permeability in Arabidopsis.

Mol Biol Rep 2020 May 28;47(5):3585-3592. Epub 2020 Apr 28.

College of Life Science, Northeast Agricultural University, Harbin, 150030, China.

In our previous study, AtDBP1 encoding a DBP factor was identified as a putative abiotic stress candidate gene. DBP factors are important regulators that participate in both transcriptional regulation and post-translational regulation, but their roles in abiotic stress are still not well-understood. So we conducted a detailed study on the function of AtDBP1 in abiotic stress. It is found that expression of AtDBP1 could be induced by drought and salt, and the induction by salt was inhibited in ABA-deficient mutant aba2-3, indicating the expression of AtDBP1 was ABA-inducible. Overexpression of AtDBP1 resulted in a rapid stomatal closure, and elevated expression of drought/salt-responsive genes, which should help Arabidopsis to enhance the drought and salt tolerance. Unexpectedly, overexpression of AtDBP1 decreased the drought and salt tolerance of Arabidopsis. Further analysis suggested that AtDBP1 is involved in cuticle wax and cuticle membrane regulation. Overexpression of AtDBP1 showed increased cuticular conductance due to a decreased cuticle wax accumulation and cuticle membrane thickness. The cuticular wax provides an essential barrier for decreasing nonstomatal water loss during drought stress, so overexpression of AtDBP1 showed decreased drought tolerance possibly ascribed to the change of cuticle membrane structure. Our previous study elucidated that AtDBP1 was also involved in flowering time regulation. Taken together, the results above indicated that AtDBP1 was involved in both plant development and stress regulation. The mechanism of AtDBP1 in this study indicates that genes involved in both plant development and stress regulation might be not suitable for production application in breeding. Collectively, our results provide some new ideas on purposefully increasing the abiotic stress without influence on plant growth and development.
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http://dx.doi.org/10.1007/s11033-020-05451-1DOI Listing
May 2020

Separation and qualitative study of L. essential oil components by prep-GC, GC-QTOF-MS and NMR.

Nat Prod Res 2020 Apr 27:1-4. Epub 2020 Apr 27.

State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, China.

In order to solve the problem of inaccuracy of using GC-QTOF-MS combined with NIST14 database in analyzing L. essential oil components, systematic isolation and characterization of chemicals from essential oil were carried on. Seventeen compounds which were 3 terpenoids, 8 lipid compounds, 3 alcohol compounds and 3 ketones were separated by preparative GC after dividing the essential oil into 5 groups of fractions by silica gel column. The chemical structures of isolated compounds were identified by GC-QTOF-MS and NMR. Three new in general compounds (50, 53 and 55) were isolated. And proton and carbon spectra of six compounds were provided for the first time. Through separation and structure identification of the compounds, qualitative analysis of total chemical components of the essential oil was increased from 42.0% to 90.3%. This research would be of great significance to application of L. essential oil.
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http://dx.doi.org/10.1080/14786419.2020.1756798DOI Listing
April 2020

Effect of Glutamine on Antioxidant Capacity and Lipid Peroxidation in the Breast Muscle of Heat-stressed Broilers via Antioxidant Genes and HSP70 Pathway.

Animals (Basel) 2020 Feb 29;10(3). Epub 2020 Feb 29.

College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China.

This study investigated whether Glutamine (Gln) could be used as an additive to improve antioxidant capacity in the breast muscle of heat-stressed broilers. Two hundred and forty 22-day-old Arbor Acres broilers in the G1, G2, G3, and G4 groups ( = 60 each) were housed in a cyclic hot environment and fed the basal diet with 0%, 0.5%, 1.0%, and 1.5% Gln, respectively. Compared with the G1 group, dietary 1.5% Gln increased ( < 0.05) pH and b* values, but decreased ( < 0.05) L* cooking loss, drip loss, and water loss rate in breast meat of heat-stressed broilers. Malondialdehyde levels in the breast muscle were lower ( < 0.05) in 1.0% and 1.5% Gln groups than that of the heat-stress group. Compared with the G1 group, dietary 1.5% Gln increased ( < 0.05) catalase (CAT), glutathione, glutathione peroxidase (GSH-Px,) and total antioxidant capacity in the breast muscle of heat-stressed broilers. Furthermore, the CAT, GSH-Px, HSP70 mRNA expression levels, and HSP70 protein expression levels were increased ( < 0.05) in the G3 and G4 groups compared with the G1 group. In sum, Gln alleviated antioxidant capacity and lipid peroxidation in the breast muscle of heat-stressed broilers through antioxidant genes and HSP70 pathways.
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http://dx.doi.org/10.3390/ani10030404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143643PMC
February 2020

Glutamine improves heat stress-induced oxidative damage in the broiler thigh muscle by activating the nuclear factor erythroid 2-related 2/Kelch-like ECH-associated protein 1 signaling pathway.

Poult Sci 2020 Mar 26;99(3):1454-1461. Epub 2019 Dec 26.

College of Animal Science, Anhui Science and Technology University, Fengyang, 233100 People's Republic of China. Electronic address:

The aim of the present study was to evaluate the effect of glutamine (Gln) on modulating heat stress-induced oxidative damage in the broiler thigh muscle through nuclear factor erythroid 2-related 2/Kelch-like ECH-associated protein 1 (Nrf2-Keap1) pathway. Three-hundred 22-day-old Arbor Acres broilers were reallocated into 5 groups: a control group (24 °C) fed with basal diet and 4 heat stress (HS) groups (34 °C for 8 h/D) fed with basal diet containing 0, 0.5, 1.0, and 1.5% Gln. This experiment lasted 21 D. Heat stress decreased (P < 0.05) pH, redness, and Gln levels, and increased (P < 0.05) luminance, water loss rate, and cooking loss (CL) values of the thigh meat. Compared with the HS group, supplementation with 1.5% Gln increased (P < 0.05) pH, redness, and Gln levels, but decreased (P < 0.05) luminance and CL values in the thigh meat. There were significant decreases (P < 0.05) in glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and Nrf2 levels, but significant increases (P < 0.05) in the malondialdehyde (MDA) and Keap1 levels of the thigh muscle after HS treatment. Compared with the HS group, supplementation with 1.0, and 1.5% Gln decreased (P < 0.05) MDA and Keap1 levels; supplementation with 1.5% Gln increased (P < 0.05) GSH, GSH-Px, T-AOC, CAT, SOD, and Nrf2 levels in the thigh muscle of heat-stressed broilers. Furthermore, HS decreased (P < 0.05) Nrf2, SOD, CAT, and GSH-Px mRNA expression levels, but increased (P < 0.05) Keap1 mRNA level in the thigh muscle of broiler. Dietary supplementation with 1.5% Gln increased (P < 0.05) Nrf2, GSH-Px, CAT, and SOD mRNA expression levels, but decreased (P < 0.05) Keap1 mRNA level in the thigh muscle of heat-stressed broilers. In conclusion, dietary Gln improved the resistance of heat-stressed broiler muscles to oxidative damage possibly through reversing the muscle Gln level and inducing the expression of the Nrf2-Keap1 pathway.
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http://dx.doi.org/10.1016/j.psj.2019.11.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587763PMC
March 2020

The impact of FIGO type 3 fibroids on in-vitro fertilization outcomes: A nested retrospective case-control study.

Eur J Obstet Gynecol Reprod Biol 2020 Apr 17;247:176-180. Epub 2020 Feb 17.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China; National Clinical Research Center for Obstetrics and Gynecology, Beijing, 100191, China; Key Laboratory of Assisted Reproduction(Peking University), Ministry of Education, Beijing, 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China. Electronic address:

Objective: To explore the impact of FIGO type 3 fibroids on the outcomes of in vitro fertilization (IVF) cycles.

Study Design: This is a nested retrospective case-control study. Women with FIGO type 3 intramural fibroids, who underwent the first IVF cycle between January 2010 and December 2016 were included in the study as fibroids group. Patients in control group were randomly selected from a general IVF cohort by a ratio of 2:1 with fibroids group, matched by age, BMI, type and cause of infertility and antral follicle count. Implantation rate (IPR), clinical pregnancy rate (CPR), miscarriage rate (MR), and live birth rate (LBR) were compared between groups.

Results: A total of 97 women comprised the fibroids group. Additional 194 women with normal uterus were included in the control group. While baseline characteristics did not differ between the groups, women with FIGO type 3 intramural fibroids showed significantly lower IPR (15.7 % vs. 24.6 %, p = 0.015), CPR (23.7 % vs. 38.1 %, p = 0.014) and LBR (16.5 % vs. 30.4 %, p = 0.011) compared with non-fibroid controls. No difference was observed in MR (p > 0.05). Fibroids of a maximum diameter ≥30 mm or multiple (≥2) fibroids decrease the IPR, CPR and LBR compared with the control group, while the smaller ones or single fibroid has no impact on IVF outcomes.

Conclusions: FIGO type 3 fibroid ≥30 mm or multiple fibroids might exert deleterious impact on implantation, clinical pregnancy and live birth rate of IVF cycles.
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http://dx.doi.org/10.1016/j.ejogrb.2019.12.018DOI Listing
April 2020

Inactivation by osmotic dehydration and air drying of Salmonella, Shiga toxin-producing Escherichia coli, Listeria monocytogenes, hepatitis A virus and selected surrogates on blueberries.

Int J Food Microbiol 2020 May 15;320:108522. Epub 2020 Jan 15.

Nestlé Research, Institute of Food Safety and Analytical Science, 1000 Lausanne 26, Switzerland. Electronic address:

Osmotically dehydrated and air dried berry fruits are used as ingredients for the production of yoghurts, chocolates, cereal bars and mixes, ice creams and cakes and these fruits are often subjected to mild thermal treatments only, posing questions around their microbiological safety. As osmotic dehydration methods and parameters vary considerably within the industry and minimally processed high quality fruits are increasingly sought, the scope of this study was to determine which temperatures are required for the inactivation of relevant bacteria and viruses during osmotic dehydration of berries, using blueberries as a model berry in a thawed state to mimic common industrial practices. Additionally, we studied the inactivation of osmotic dehydration at 23 °C, sometimes referred to "cold infusion" followed by air drying at 100 °C to determine the microbiological safety achieved by this combined treatment. Four pathogens (Salmonella enterica, Escherichia coli O157:H7, Listeria monocytogenes and hepatitis A virus (HAV)) and five surrogates (Enterococcus faecium, Escherichia coli P1, Listeria innocua, murine Norovirus (MNV) and bacteriophage MS2) were inoculated on blueberries and reductions were measured after different treatment combinations. After osmotic dehydration of bacterial strains at 40 °C no survivors were detected on blueberries, with the exception of E. faecium. Inactivation of the viruses at 45 °C showed no survivors for MS2 and mean reductions of 1.5 and 3.4 log median tissue culture infectious dose (TCID)/g for HAV and MNV, respectively. Similarly, in the sugar solution at 40 °C, no survivors were observed, with the exception of E. faecium and the three viruses. The combined process (osmotic dehydration at 23 °C followed by air-drying at 100 °C) achieved an >6 log reduction of all tested bacterial strains and MS2. For HAV and MNV, 2.6 and >3.4 log TCID/g were measured. In summary, the present study shows that osmotic dehydration appears an efficient control measure for the control of L. monocytogenes, S. enterica and E. coli O157:H7 if carried out at 40 °C or at 23 °C and followed by air-drying at 100 °C. Based on the results generated with MNV, the combined treatment is also expected to reduce human Norovirus (NoV) but does not appear to be sufficient to fully control HAV. The results contribute to a better management of the microbial safety of osmotically dehydrated and dried berries and especially the results generated for the viruses emphasize that within a robust food safety management system, safety must be assured through the entire food supply chain and therefore must start at primary production with the implementation of Good Agricultural Practices (GAP).
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http://dx.doi.org/10.1016/j.ijfoodmicro.2020.108522DOI Listing
May 2020

Different Therapeutic Effects of Transcranial Direct Current Stimulation on Upper and Lower Limb Recovery of Stroke Patients with Motor Dysfunction: A Meta-Analysis.

Neural Plast 2019 16;2019:1372138. Epub 2019 Nov 16.

Department of Radiology and Imaging Institute of Rehabilitation and Development of Brain Function, The Second Clinical Medical College of North Sichuan Medical College Nanchong Central Hospital, Nanchong, China.

Objective: To explore the effects of transcranial direct current stimulation (tDCS) on the motor recovery of stroke patients and the effect differences between the upper limb and lower limb.

Methods: Randomized control trials published until January 2019 were searched from PubMed, Embase, ScienceDirect, and Cochrane Library databases. The standardized mean difference (SMD) with 95% confidence interval (CI) was estimated separately for upper and lower limb motor outcomes to understand the mean effect size.

Results: Twenty-nine studies with 664 subjects were included in this meta-analysis. The overall analyses of tDCS demonstrated significant effect size both for the upper limb (SMD = 0.26, = 0.002) and the lower limb (SMD = 0.47, = 0.002). Compared with acute and subacute stroke patients, chronic stroke patients obtained significant effects after tDCS (SMD = 0.25, = 0.03) in upper limb function. Furthermore, both anode and cathode stimulations produced significant effect size for stroke patients after ≤10 sessions of tDCS (anode: SMD = 0.40, = 0.001; cathode: SMD = 0.79, < 0.0001) with >0.029 mA/cm of density (anode: SMD = 0.46, = 0.002; cathode: SMD = 0.79, < 0.0001). But for lower limb function, more prominent effects were found in subacute stroke patients (SMD = 0.56, = 0.001) with bilateral tDCS (SMD = 0.59, = 0.009).

Conclusion: tDCS is effective for the recovery of stroke patients with motor dysfunction. In addition, upper limb and lower limb functions obtain distinct effects from different therapeutic parameters of tDCS at different stages, respectively.
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http://dx.doi.org/10.1155/2019/1372138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881758PMC
July 2020

Endorsement of Animal Research: Incorporation of In Vivo Experiments (ARRIVE) Guidelines/Gold Standard Publication Checklist (GSPC) by Chinese journals: A survey of journals' instructions for authors and editors.

Lab Anim 2019 Oct 9:23677219879181. Epub 2019 Oct 9.

Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, China.

The objective was to determine the rate at which Chinese journals include Animal Research: Reporting of In Vivo Experiments (ARRIVE) Guidelines/Gold Standard Publication Checklist (GSPC) in their instructions for authors, and the awareness and recognition of editors. The survey was performed on Chinese journals. The most recent versions each journal's instructions for authors were downloaded, and the information related to the ARRIVE/GSPC was collected. A self-developed questionnaire was used to conduct the survey among the editors. Questionnaires were sent to 238 qualified journals and 198 of them returned them, achieving an 83.2% response rate. The results showed that none of the journals included the ARRIVE/GSPC in their instructions for authors, and the awareness rate was only 13.1% (26/198). The participants who were unaware of the ARRIVE/GSPC were less likely than those who were aware of them to believe it was necessary to include the ARRIVE/GSPC in the instructions for authors (23.3% . 61.5%), and less likely to request authors in their manuscript preparation (28.5% . 88.5%), editors in the editing and processing (28.5% . 84.6%) and reviewers in peer review stage (28.5% . 92.3%) to follow the ARRIVE/GSPC. Currently no Chinese journals include the ARRIVE/GSPC in their instructions for authors. The recognition rate of the ARRIVE/GSPC was low among the editors. So, we suggest that Chinese journals should promote inclusion of the ARRIVE/GSPC in journals' instructions for authors. It is also important to educate researchers and editors alike to increase their understanding of the ARRIVE/GSPC, so that the quality of reporting of animal study can be improved.
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http://dx.doi.org/10.1177/0023677219879181DOI Listing
October 2019

Efficacy and safety of long-acting growth hormone in children with short stature: a systematic review and meta-analysis.

Endocrine 2019 07 22;65(1):25-34. Epub 2019 May 22.

Department of Endocrinology, Key Lab of Endocrinology, Ministry of Health, Peking Union Medical College Hospital (PUMCH), Chinese Academe of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Beijing, 100730, China.

Purpose: Long-acting growth hormone (GH) has been developed to address the noncompliance and decreased efficacy associated with daily GH injections. We aimed to evaluate the efficacy and safety of long-acting GH replacement therapy in children with short stature.

Methods: Randomized controlled trials (RCTs) that investigated the efficacy and safety of long-acting GH therapy in children with short stature in comparison with daily GH injections were searched in Medline, Embase, and the Cochrane Central Register of Controlled Trials. A random-effect model was used to pool data using mean difference and odds ratios (OR). (PROSPERO registration number: CRD42018111105).

Results: Seven relevant studies were finally included. Meta-analysis found there was no significant difference between high-dose long-acting GH and daily GH in terms of height velocity (HV) (mean difference (MD) = -0.10, 95% CI, -0.79 to 0.60, P = 0.79). Moreover, no significant difference was observed in height standard deviation scores (Ht SDS) between high-dose long-acting GH and daily GH (MD = -0.07, 95% CI, -0.18 to 0.03, P = 0.17). Treatment with high-dose long-acting GH significantly increased IGF-1 SDS when compared with daily GH (MD = 0.31, 95% CI, 0.06-0.56, P = 0.02). In safety assessment, no significant difference was observed in the incidence of adverse events between high-dose long-acting GH and daily GH (OR 1.42, 95% CI, 0.65-3.11, P = 0.38).

Conclusions: There is no evidence to support differences in the effects of long-acting GH compared with those of daily GH. More RCTs that focus on the safety of high-dose long-acting GH treatment, especially the detection of adverse events caused by elevated levels of serum IGF-1, are needed in the future.
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http://dx.doi.org/10.1007/s12020-019-01950-9DOI Listing
July 2019

Efficient Expression of Xylanase by Codon Optimization and Its Effects on the Growth Performance and Carcass Characteristics of Broiler.

Animals (Basel) 2019 Feb 20;9(2). Epub 2019 Feb 20.

College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China.

The aim of the present study was to improve the expression level of xylanase (XynB) in through a codon optimization strategy and evaluate its effects on the growth performance and carcass characteristics of broiler. According to the codon bias of genome, the gene from was optimized and synthesized by whole gene assembly to improve its expression level in . Approximately 180 target mutations were successfully introduced into natural . The maximum activity of xylanase (optiXynB) secreted by pPICZaA-optiXynB was 1299 U/mL after 96 h induction. Purified recombinant optiXynB had the molecular weight of 24 kDa. The optiXynB presented highest activity in pH 5.0 and 50 °C. The recombinase was highly specific towards birchwood xylan, beechwood xylan, and oat-spelt xylan. In the broiler experiment, a total of 200 Arbor Acre broilers (one day old) were randomly allocated into four groups fed with basal diets containing 0 (control group), 500, 1000, and 1500 IU/kg optiXynB. Dietary 1000 and 1500 IU/kg optiXynB significantly increased ( < 0.05) final weight and body weight gain; dietary 500, 1000, and 1500 IU/kg optiXynB significantly increased ( < 0.05) pre-evisceration weight, dressed percentage, and eviscerated weight compared with the control group. Inclusion of optiXynB in broiler diets linearly increased final weight, body weight gain, breast muscle weight and leg muscle weight, but linearly decreased feed conversion rate ( < 0.05). Furthermore, inclusion of optiXynB in broiler diets linearly and quadratically increased pre-evisceration weight, dressed percentage, and eviscerated weight ( < 0.05). The recombinant optiXynB from pPICZaA-optiXynB was beneficial in improving growth performance and carcass characteristics of broilers.
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http://dx.doi.org/10.3390/ani9020065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406647PMC
February 2019

Qualitative analysis of Schizonepeta annua (Pall.) Schischk essential oil by gas chromatography-quadrupole time-of-flight mass spectrometry.

Eur J Mass Spectrom (Chichester) 2018 Dec 2;24(6):454-462. Epub 2018 Aug 2.

1 Xinjiang Technical Institute of Physics and Chemistry, and State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, China.

In this study, a method for the qualitative analysis of small molecular compounds in Schizonepeta annua (Pall.) Schischk essential oil was established based on gas chromatography-quadrupole time-of-flight mass spectrometry. In addition to an automated search of the NIST library, the identification of oxygenated monoterpenes, phenolic esters, and phenolic compounds was achieved by two additional strategies. One strategy involved comparing the relative errors of accurate masses measured for ions in the experimental spectra with those calculated for fragments identified from the NIST database of candidate matches. The second strategy involved combination of the product ion scans and positive chemical ionisation spectra for structural elucidation. Overall, 95.45% of the total essential oil volatile chemical content of Schizonepeta annua (Pall.) Schischk was identified, with phenolic monoterpenes dominating.
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http://dx.doi.org/10.1177/1469066718791789DOI Listing
December 2018

Improvement of imprinting effect of ionic liquid molecularly imprinted polymers by use of a molecular crowding agent.

Anal Bioanal Chem 2018 Jan 19;410(2):595-604. Epub 2017 Dec 19.

Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, Xinjiang, 830011, China.

We aimed to improve the imprinting effect of ionic liquid molecularly imprinted polymers (MIPs) by use of a molecular crowding agent. The ionic liquid 1-vinyl-3-ethylimidazolium tetrafluoroborate ([VEIm][BF]) was used as the functional monomer and aesculetin was used as the template molecule in a crowding environment, which was made up of a tetrahydrofuran solution of polystyrene. The ionic liquid MIPs that were prepared in the crowding environment displayed an enhanced imprinting effect. NMR peak shifts of active hydrogen of aesculetin suggested that interaction between the functional monomer and the template could be increased by the use of a crowding agent in the self-assembly process. The retention and selectivity of aesculetin were affected greatly by high molecular crowding, the amount of high molecular weight crowding agent, and the ratio of [VEIm][BF] to aesculetin. The optimal MIPs were used as solid-phase extraction sorbents to extract aesculetin from Cichorium glandulosum. A calibration curve was obtained with aesculetin concentrations from 0.0005 to 0.05 mg mL (correlation coefficient R of 0.9999, y = 1519x + 0.0923). The limit of quantification was 0.12 μg mL, and the limit of detection was 0.05 μg mL. The absolute recovery of aesculetin was (80 ± 2)% (n = 3), and the purity of aesculetin was (92 ± 0.5)% (n = 5). As a conclusion, molecular crowding is an effective approach to obtain ionic liquid MIPs with high selectivity even in a polar solvent environment.
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http://dx.doi.org/10.1007/s00216-017-0760-5DOI Listing
January 2018

Cloning, expression and characterization of a cold-adapted endo-1, 4--glucanase from A1, a symbiotic bacterium of .

PeerJ 2016 8;4:e2679. Epub 2016 Nov 8.

Institute of Ensiling and Processing of Grass, Nanjing Agricultural University , Nanjing , China.

Background: Many biotechnological and industrial applications can benefit from cold-adapted EglCs through increased efficiency of catalytic processes at low temperature. In our previous study, A1 which was isolated from a wood-inhabiting termite could secrete a cold-adapted EglC. However, its EglC was difficult to purify for enzymatic properties detection because of its low activity (0.8 U/ml). The objective of the present study was to clone and express the gene in to improve production level and determine the enzymatic properties of the recombinant enzyme.

Methods: The gene was cloned from A1 by thermal asymmetric interlaced PCR. was transformed into vector pET22b and functionally expressed in . The recombination protein EglC22b was purified for properties detection.

Results: SDS-PAGE revealed that the molecular mass of the recombinant endoglucanase was approximately 42 kDa. The activity of the pET22b-EglC crude extract was 9.5 U/ml. Additionally, it was active at pH 6.5-8.0 with an optimum pH of 7.0. The recombinant enzyme had an optimal temperature of 30-40 °C and exhibited >50% relative activity even at 5 °C, whereas it lost approximately 90% of its activity after incubation at 60 °C for 30 min. Its activity was enhanced by Co and Fe, but inhibited by Cd, Zn, Li, Triton X-100, DMSO, acetonitrile, Tween 80, SDS, and EDTA.

Conclusion: These biochemical properties indicate that the recombinant enzyme is a cold-adapted endoglucanase that can be used for various industrial applications.
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http://dx.doi.org/10.7717/peerj.2679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103814PMC
November 2016

Inhibiting receptor tyrosine kinase AXL with small molecule inhibitor BMS-777607 reduces glioblastoma growth, migration, and invasion in vitro and in vivo.

Oncotarget 2016 Mar;7(9):9876-89

Department of Neurosurgery, Charité, Berlin, Germany.

Purpose: Receptor tyrosine kinase AXL (RTK-AXL) is regarded as suitable target in glioma therapy. Here we evaluate the anti-tumoral effect of small molecule inhibitor BMS-777607 targeting RTK-AXL in a preclinical glioma model and provide evidence that RTK-AXL is expressed and phosphorylated in primary and recurrent glioblastoma multiforme (GBM).

Experimental Design: We studied the impact of BMS-777607 targeting RTK-AXL in GBM models in vitro and in vivo utilizing glioma cells SF126 and U118MG. Impact on proliferation, apoptosis and angiogenesis was investigated by immunohistochemistry (IHC) and functional assays in vitro and in vivo. Tumor growth was assessed with MRI. Human GBM tissue was analyzed in terms of RTK-AXL phosphorylation by immunoprecipitation and immunohistochemistry.

Results: BMS-777607 displayed various anti-cancer effects dependent on increased apoptosis, decreased proliferation and migration in vitro and ex vivo in SF126 and U118 GBM cells. In vivo we observed a 56% tumor volume reduction in SF126 xenografts and remission in U118MG xenografts of more than 91%. The tube formation assay confirmed the anti-angiogenic effect of BMS-777607, which became also apparent in tumor xenografts. IHC of human GBM tissue localized phosphorylated RTK-AXL in hypercellular tumor regions, the migratory front of tumor cells in pseudo-palisades, and in vascular proliferates within the tumor. We further proved RTK-AXL phosphorylation in primary and recurrent disease state.

Conclusion: Collectively, these data strongly suggest that targeting RTK-AXL with BMS-777607 could represent a novel and potent regimen for the treatment of primary and recurrent GBM.
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http://dx.doi.org/10.18632/oncotarget.7130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891090PMC
March 2016

Interactive effects of glutamine and gamma-aminobutyric acid on growth performance and skeletal muscle amino acid metabolism of 22-42-day-old broilers exposed to hot environment.

Int J Biometeorol 2016 Jun 22;60(6):907-15. Epub 2015 Oct 22.

College of Animal Science, Anhui Science and Technology University, No. 9 Donghua road, Fengyang, 233100, People's Republic of China.

The present experiment was conducted to investigate the interactive effects between dietary glutamine (Gln, 0 and 5 g/kg) and gamma-aminobutyric acid (GABA, 0 and 100 mg/kg) on growth performance and amino acid (AA) metabolism of broilers under hot environment. A total of 360 22-day-old Arbor Acres male chickens were randomly assigned to five treatment groups under thermoneutral chamber (PC, 23 °C) and cyclic heat stress (HS, 30-34 °C cycling) conditions. Compared with the PC group, cyclic HS decreased (P < 0.05) daily weight gain (DWG), daily feed consumption (DFC), the concentrations of Gln, glutamate (Glu), and GABA, and the activities of glutaminase and glutamic acid decarboxylase (GAD) in breast muscle at 28, 35, and 42 days, while it increased (P < 0.05) the activities of glutamine synthetase (GS) and gamma-aminobutyric acid transaminase (GABA-T) at 28, 35, and 42 days. Dietary Gln and GABA improved (P < 0.05) DWG and DFC of broilers under cyclic HS during 28-42 days. In breast muscle, the Gln supplementation increased (P < 0.05) the concentrations of Gln (28, 35, and 42 days), Glu (28, 35, and 42 days), and GABA (42 days) and the activities of glutaminase (28, 35, and 42 days) and GAD (28, 35, and 42 days) but decreased (P < 0.05) GS activities at 28, 35, and 42 days and GABA-T activities at 28 days. The addition of GABA increased (P < 0.05) the concentrations of Gln and Glu and activities of glutaminase and GAD, while it decreased (P < 0.05) GABA-T activities at 28, 35, and 42 days. Significant interactions (P < 0.05) between Gln and GABA were found on breast skeletal muscle Gln concentrations, glutaminase activities, GS activities at 28 and 35 days, and DWG, GABA concentrations, and GABA-T activities at 28, 35, and 42 days in broilers under cyclic HS. In conclusion, the present results indicated that the interactions of exogenous Gln and GABA could offer a potential nutritional strategy to prevent HS-related depression in skeletal muscle Gln and GABA metabolism of broilers.
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http://dx.doi.org/10.1007/s00484-015-1084-9DOI Listing
June 2016

Wild soybean roots depend on specific transcription factors and oxidation reduction related genesin response to alkaline stress.

Funct Integr Genomics 2015 Nov 15;15(6):651-60. Epub 2015 Apr 15.

Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, 150030, China.

Soil alkalinity is an important environmental problem limiting agricultural productivity. Wild soybean (Glycine soja) shows strong alkaline stress tolerance, so it is an ideal plant candidate for studying the molecular mechanisms of alkaline tolerance and identifying alkaline stress-responsive genes. However, limited information is available about G. soja responses to alkaline stress on a genomic scale. Therefore, in the present study, we used RNA sequencing to compare transcript profiles of G. soja root responses to sodium bicarbonate (NaHCO3) at six time points, and a total of 68,138,478 pairs of clean reads were obtained using the Illumina GAIIX. Expression patterns of 46,404 G. soja genes were profiled in all six samples based on RNA-seq data using Cufflinks software. Then, t12 transcription factors from MYB, WRKY, NAC, bZIP, C2H2, HB, and TIFY families and 12 oxidation reduction related genes were chosen and verified to be induced in response to alkaline stress by using quantitative real-time polymerase chain reaction (qRT-PCR). The GO functional annotation analysis showed that besides "transcriptional regulation" and "oxidation reduction," these genes were involved in a variety of processes, such as "binding" and "response to stress." This is the first comprehensive transcriptome profiling analysis of wild soybean root under alkaline stress by RNA sequencing. Our results highlight changes in the gene expression patterns and identify a set of genes induced by NaHCO3 stress. These findings provide a base for the global analyses of G. soja alkaline stress tolerance mechanisms.
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http://dx.doi.org/10.1007/s10142-015-0439-yDOI Listing
November 2015

Expression of a β-mannosidase from Paenibacillus polymyxa A-8 in Escherichia coli and characterization of the recombinant enzyme.

PLoS One 2014 25;9(11):e111622. Epub 2014 Nov 25.

College of Science, Sichuan Agricultural University, Yaan, Sichuan 625000, P. R. China.

Paenibacillus polymyxa A-8, which secretes β-mannosidase, was isolated from the soil sample under a pine tree located in the "Laoban" mountain region of Sichuan, China. The β-mannosidase gene (MANB) was isolated from P. polymyxa A-8, using primers according to the complete genome. The MANB (2,550 bp) encoding 849 amino acid residues was expressed in Escherichia coli. The specific activities of β-mannosidase produced by P. polymyxa A-8 and E. coli pET30a-MANB were 12 nkat/mg and 635 nkat/mg respectively. SDS-PAGE analysis indicated that the molecular mass of the recombinant MANB was approximately 96 kDa. The recombinant MANB was active between pH 7.0-8.5 with the maximum activity at pH 7.0. It had good pH stability and adaptability. The MANB had the optimal temperature of 35°C and was relatively stable at 35-40°C. In addition, the MANB activity was enhanced by K+, Ca2+, Mn2+, and Mg2+ and inhibited by Zn2+, Cu2+, and Hg2+.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0111622PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244029PMC
July 2015

Ectopic overexpression of a novel Glycine soja stress-induced plasma membrane intrinsic protein increases sensitivity to salt and dehydration in transgenic Arabidopsis thaliana plants.

J Plant Res 2015 Jan 31;128(1):103-13. Epub 2014 Oct 31.

The Key Laboratory of Sugar Beet Genetic Breeding, Colleges of Heilongjiang Province, Heilongjiang University, No. 74 XueFu Road, NanGang District, Harbin, 150080, Heilongjiang, China,

Plasma membrane intrinsic proteins (PIPs) belong to the aquaporin family and facilitate water movement across plasma membranes. Existing data indicate that PIP genes are associated with the abilities of plants to tolerate certain stress conditions. A review of our Glycine soja expressed sequence tag (EST) dataset revealed that abiotic stress stimulated expression of a PIP, herein designated as GsPIP2;1 (GenBank_Accn: FJ825766). To understand the roles of this PIP in stress tolerance, we generated a coding sequence for GsPIP2;1 by in silico elongation and cloned the cDNA by 5'-RACE. Semiquantitative RT-PCR showed that GsPIP2;1 expression was stimulated in G. soja leaves by cold, salt, or dehydration stress, whereas the same stresses suppressed GsPIP2;1 expression in the roots. Transgenic Arabidopsis thaliana plants overexpressing GsPIP2;1 grew normally under unstressed and cold conditions, but exhibited depressed tolerance to salt and dehydration stresses. Moreover, greater changes in water potential were detected in the transgenic A. thaliana shoots, implying that GsPIP2;1 may negatively impact stress tolerance by regulating water potential. These results, deviating from those obtained in previous reports, provide new insights into the relationship between PIPs and abiotic stress tolerance in plants.
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http://dx.doi.org/10.1007/s10265-014-0674-7DOI Listing
January 2015

Effect of bulk density of coking coal on swelling pressure.

J Environ Sci (China) 2013 Dec;25 Suppl 1:S205-9

ACRE Coking & Refractory Engineering Consulting Corporation, MCC, Anshan 104002, China.

Coking coals are the important raw materials for the iron and steel industries and play an important role on its sustainable development, especially on the stamp-charging coke making with the characteristics of increasing the bulk density. There is a significance on the reasonable usage of the coking coal resource with the reduced production cost, improved efficiency of the economy to develop the stamp-charging coke making technology. Important effects of the density of coking coal on the coking and caking properties were investigated. In the article, the maximum values of swelling pressure and variation of Laowan gas coal and Xinjian 1/3 coking coal, Longhu fat coal and Didao coking coal, which were mined at Shenyang and Qitaihe respectively, were investigated under different bulk densities during the coking. The results showed that when the values of density increased from 0.85 ton/m(3) to 1.05 ton/m(3), for the Laowan gas coal, swelling pressure variation and even the maximum value changed slightly. The swelling pressure was 3.63 KPa when the density was improved to 1.05 ton/m(3); for the Xinjian 1/3 coking coal, the values of swelling pressure changed significantly and the maximum values was 82.88 KPa with the density improved to 1.05 when the coal was heated to 600°C. The coke porosity, which was investigated by automatic microphotometer, decreased from 47.4% to 33.1% with the increasing of the density from 0.85 ton/m(3) to 1.05 ton/m(3), and the decreased value was 14.3%. Meanwhile, the pore structures of four cokes were characterized by an optical microscope.
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http://dx.doi.org/10.1016/S1001-0742(14)60657-4DOI Listing
December 2013

Removement of thiocyanate from industrial wastewater by microwave-Fenton oxidation method.

J Environ Sci (China) 2013 Dec;25 Suppl 1:S201-4

School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China.

The microwave radiation oxidation process, Fenton as catalytic agent, was used to remove the thiocyanate from the industrial wastewater. The effects of microwave power, radiation time, pH and the feeding in ways of catalyst on the degradation rate of synthetic wastewater were investigated using the microwave radiation oxidation process by orthogonal experiment. The results show Fenton catalyst ratio was 1:20, the microwave radiation power was 900 W, the microwave radiation time was 7 min and the value of pH was 3. Under the optimum conditions, the removal of KSCN can reach over 90%. The apparent kinetics of removal was studied, which conformed to kinetics first-class reaction. In short, for the thiocyanate from the industrial wastewater, microwave-Fenton oxidation method is feasible and effective.
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http://dx.doi.org/10.1016/S1001-0742(14)60656-2DOI Listing
December 2013

Research on the evolvement of morphology of coking coal during the coking process.

J Environ Sci (China) 2013 Dec;25 Suppl 1:S186-9

Key Lab of Advanced Coal and Coking Technology Liaoning, School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114053, China.

The evolvement of morphology and structure of the coal with different metamorphic degrees during coking process in the vertical furnace was investigated by infrared Image detector. Moreover, the temperature distribution in the radial direction and the crack formation were also studied in heating process. The results show that the amount of crack and the shrinkage level of char decrease with the coal rank rising. In addition, the initial temperature of crack formation for char increases with the coal rank rising.
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http://dx.doi.org/10.1016/S1001-0742(14)60653-7DOI Listing
December 2013

Overexpression of GsCBRLK from Glycine soja enhances tolerance to salt stress in transgenic alfalfa (Medicago sativa).

Funct Plant Biol 2013 Oct;40(10):1048-1056

College of Life Science, Northeast Agricultural University, Harbin 150030, China.

GsCBRLK encodes a novel plant-specific calcium-dependent calmodulin-binding receptor-like kinase from Glycine soja Siebold & Zucc. In our previous study, GsCBRLK was found to be a positive regulator of plant tolerance to salt and abscisic acid (ABA) stress. In this study we transformed alfalfa (Medicago sativa L.) with GsCBRLK to assess whether forage legumes overexpressing GsCBRLK adapt to saline soils. Results showed that transgenic alfalfa plants overexpressing GsCBRLK exhibited enhanced salt tolerance. Transgenic alfalfa grew well in the presence of 300mM NaCl for 15 days, whereas wild-type (WT) plants exhibited severe chlorosis and growth retardation. Although transgenic alfalfa grew slowly and even had yellow leaves under the 400mM NaCl treatment, most of the WT plants exhibited more severe chlorosis and did not survive. In addition, samples from transgenic and WT plants treated with 300mM NaCl for 0, 3, 6, 9, 12, and 15 days were selected for physiological analysis. Lower membrane leakage and malondialdehyde (MDA) content were observed in transgenic alfalfa compared with WT plants during salt treatment. The reduction of chlorophyll content in transgenic alfalfa was less than that in WT plants. Furthermore, the plants that overexpressed GsCBRLK showed enhanced superoxide dismutase (SOD) activity, less of a Na+ increase, and a greater K+ decrease than WT plants. These results indicated that the overexpression of GsCBRLK confers enhanced tolerance to salt stress in transgenic alfalfa.
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http://dx.doi.org/10.1071/FP12377DOI Listing
October 2013
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