Publications by authors named "Woo Joo"

78 Publications

Corrigendum to "Effectiveness and safety of early enteral nutrition for patients who received targeted temperature management after out-of-hospital cardiac arrest" [Resuscitation 135 (2019) 191-196].

Resuscitation 2021 Mar 4;160:70-71. Epub 2021 Feb 4.

Department of Pharmacoepidemiology, Graduate School of Medicine and Public Health, Kyoto University, Yoshidakonoecho, Sakyo-ku, Kyoto, Japan; Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Kyoto, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.resuscitation.2021.01.022DOI Listing
March 2021

Prediction of the neurological outcome using regional cerebral oxygen saturation in patients with extracorporeal cardiopulmonary resuscitation after out-of-hospital cardiac arrest: a multicenter retrospective cohort study.

Acute Med Surg 2020 Jan-Dec;7(1):e491. Epub 2020 Feb 28.

Department of Pharmacoepidemiology Graduate School of Medicine and Public Health Kyoto University Kyoto Japan.

Aim: To investigate the association between regional cerebral oxygen saturation (rSO2) and neurological outcomes in extracorporeal cardiopulmonary resuscitation (ECPR) patients after out-of-hospital cardiac arrest (OHCA).

Methods: We used data from the Japan-Prediction of Neurological Outcomes in Patients Post-Cardiac Arrest Registry. This registry included consecutive comatose patients after OHCA who were transferred to 15 hospitals in Japan from 2011 to 2013. Our primary end-point was a good neurological outcome (cerebral performance categories 1 or 2) at 90 days after OHCA.

Results: Among the enrolled patients, 121 (6.3%) received ECPR. Eleven (9.1%) had a good neurological outcome. Receiver operating characteristic curve analysis revealed the optimal cut-off value as >16%. Good neurological outcomes were observed in 19.6% (9/46) and 2.7% (2/74) of patients with rSO2 >16% and rSO2 ≤16%, respectively.

Conclusion: The neurological outcome of ECPR patients differed according to their rSO2 values. When considering ECPR, the rSO2 value could be important in addition to other criteria. Further studies that focus on ECPR patients and serial rSO2 values are needed.
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http://dx.doi.org/10.1002/ams2.491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774292PMC
February 2020

Nootkatol prevents ultraviolet radiation-induced photoaging ORAI1 and TRPV1 inhibition in melanocytes and keratinocytes.

Korean J Physiol Pharmacol 2021 Jan;25(1):87-94

Department of Physiology, Dongguk University College of Medicine, Gyeongju 38066, Korea.

Skin photoaging occurs due to chronic exposure to solar ultraviolet radiation (UV), the main factor contributing to extrinsic skin aging. Clinical signs of photoaging include the formation of deep, coarse skin wrinkles and hyperpigmentation. Although melanogenesis and skin wrinkling occur in different skin cells and have different underlying mechanisms, their initiation involves intracellular calcium signaling via calcium ion channels. The ORAI1 channel initiates melanogenesis in melanocytes, and the TRPV1 channel initiates MMP-1 production in keratinocytes in response to UV stimulation. We aimed to develop a drug that may simultaneously inhibit ORAI1 and TRPV1 activity to help prevent photoaging. We synthesized nootkatol, a chemical derivative of valencene. TRPV1 and ORAI1 activities were measured using the whole-cell patch-clamp technique. Intracellular calcium concentration [Ca]i was measured using calcium-sensitive fluorescent dye (Fura-2 AM). UV-induced melanin formation and MMP-1 production were quantified in B16F10 melanoma cells and HaCaT cells, respectively. Our results indicate that nootkatol (90 μM) reduced TRPV1 current by 94% ± 2% at -60 mV and ORAI1 current by 97% ± 1% at -120 mV. Intracellular calcium signaling was significantly inhibited by nootkatol in response to ORAI1 activation in human primary melanocytes (51.6% ± 0.98% at 100 μM). Additionally, UV-induced melanin synthesis was reduced by 76.38% ± 5.90% in B16F10 melanoma cells, and UV-induced MMP-1 production was reduced by 59.33% ± 1.49% in HaCaT cells. In conclusion, nootkatol inhibits both TRPV1 and ORAI1 to prevent photoaging, and targeting ion channels may be a promising strategy for preventing photoaging.
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http://dx.doi.org/10.4196/kjpp.2021.25.1.87DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756533PMC
January 2021

Photocatalytic hydrogen production from dye contaminated water and electrochemical supercapacitors using carbon nanohorns and TiO nanoflower heterogeneous catalysts.

J Environ Manage 2021 Jan 15;277:111433. Epub 2020 Oct 15.

Soniya College of Pharmacy, Dharwad, Karnataka, 580 002, India. Electronic address:

In this research, efficient and novel catalysts based on hierarchical carbon nanohorns-titanium nanoflowers have been prepared by one-pot solvothermal process. Hydrogen generation from dye-contaminated water and dye degradation along with electrochemical supercapacitance performance have been investigated using the synthesized hierarchical catalyst to produce 4500 μmol g h of hydrogen from the photocatalytically generated aqueous methylene blue and methyl orange dyes, which were degraded up to 90% under natural solar light irradiation. These results offer a new path to generate hydrogen from the aqueous dyes. The catalysts electrode showed 164.6 F g supercapacitance at 5 mV s scan rate, which is nearly 1.3 and 1.65-times higher than that of pristine titanium nanoflower and carbon nanohorns electrodes, respectively. Such superior results were achieved due to good crystallinity, improved optical absorption strength, strong chemical composition between the two components, and hierarchical morphology as demonstrated from XRD, UV-DRS, TEM, XPS, and Raman spectral characterizations.
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http://dx.doi.org/10.1016/j.jenvman.2020.111433DOI Listing
January 2021

Gold nanostar-enhanced electrochemiluminescence immunosensor for highly sensitive detection of cancer stem cells using CD133 membrane biomarker.

Bioelectrochemistry 2021 Feb 14;137:107633. Epub 2020 Aug 14.

School of Mechanical Engineering, Yeungnam University, Gyeongsan 712-749, South Korea. Electronic address:

Gold nanostars (AuNSs) demonstrate an intense electromagnetic field around tip of branches. In this research, we employed AuNSs-enhanced electrochemiluminescence (ECL) emission from graphitic carbon nitride nanosheets (g-CN nanosheets) to detect CD133 peptide as a cancer stem cell membrane biomarker. In this biosensor, the g-CN nanosheets were decorated with AuNSs (AuNSs@g-CN nanosheets). AuNSs@g-CN nanosheets exhibited strong and stable cathodic ECL emission compared to that of pure g-CN nanosheets. The ECL intensity from the AuNSs@g-CN nanosheets was over 30% higher than that of spherical gold nanoparticles (spherical AuNPs) decorated g-CN nanosheets. The additional ECL enhancement of AuNSs was due to the localized surface plasmon resonance (LSPR) effect located around multiple branch tips of AuNSs. The RSD of ECL curves intensities, obtained from successive potential scans for 10 cycles, were less than 4%, indicating the superior stability of the AuNSs@g-CN nanosheets. Under optimum conditions, the ECL intensity of GCE/AuNSs@g-CN nanosheets/anti-CD133 decreased linearly with CD133 peptide concentration in the range of 0.05-100 ng mL. The LOD achieved was 0.257 ng mL (S/N = 3). The applicability of the designed biosensor in real samples was examined through the determination of CD133 peptide in spiked serum samples, from which satisfactory results were obtained.
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http://dx.doi.org/10.1016/j.bioelechem.2020.107633DOI Listing
February 2021

Guidelines for the Management of Unilateral Vocal Fold Paralysis From the Korean Society of Laryngology, Phoniatrics and Logopedics.

Clin Exp Otorhinolaryngol 2020 Nov 4;13(4):340-360. Epub 2020 Sep 4.

Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

The Korean Society of Laryngology, Phoniatrics and Logopedics appointed a task force to establish clinical practice guidelines for the management of unilateral vocal fold paralysis (UVFP). These guidelines cover a comprehensive range of management-related factors, including the diagnosis and treatment of UVFP, and provide in-depth information based on current, up-to-date knowledge. Detailed evidence profiles are provided for each recommendation. The CORE databases, including OVID Medline, Embase, the Cochrane Library, and KoreaMed, were searched to identify all relevant papers, using a predefined search strategy. When insufficient evidence existed, expert opinions and Delphi questionnaires were used to fill the evidence gap. The committee developed 16 evidence-based recommendations in six categories: initial evaluation (R1-4), spontaneous recovery (R5), medical treatment (R6), surgical treatment (R7-14), voice therapy (R15), and aspiration prevention (R16). The goal of these guidelines is to assist general otolaryngologists and speech-language pathologists who are primarily responsible for treating patients with UVFP. These guidelines are also intended to facilitate understanding of the condition among other health-care providers, including primary care physicians, nurses, and policy-makers.
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http://dx.doi.org/10.21053/ceo.2020.00409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669319PMC
November 2020

Tension pneumoperitoneum caused by intestinal perforation from underlying colon cancer: a case report.

J Med Case Rep 2020 Jul 22;14(1):112. Epub 2020 Jul 22.

Emergency Department, Kobe City Medical Center General Hospital, 2-1-1 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo, Japan.

Background: Tension pneumoperitoneum, a form of abdominal compartment syndrome, is an important clinical condition. Increased pressure in the intraperitoneal cavity leads to respiratory and circulatory instability. Most of the reported cases include complications due to active air infusion into the peritoneal cavity or trauma; however, few reports are available on tension pneumoperitoneum caused by perforation from colon cancer. We present a case of a patient with tension pneumoperitoneum caused by gastrointestinal perforation owing to colon cancer.

Case Presentation: A 63-year-old Japanese man with altered mental state was brought to our emergency department. He was in shock, and an abdominal radiograph suggested gastrointestinal perforation. Despite rapid fluid infusion and inotropic support, his condition deteriorated. His abdomen was tensely distended; abdominal computed tomography showed significant intra-abdominal gas. Following immediate needle decompression, his circulatory status improved. Emergent laparotomy revealed an approximately 10-cm tumor (adenocarcinoma) in the colon, which caused the perforation.

Conclusions: A perforated wall or the surrounding omental fat that acts as a one-way valve could lead to tension pneumoperitoneum without active air infusion. Although tension pneumoperitoneum is a life-threatening condition, it is reversible if prompt diagnosis and immediate decompression are performed. Physicians should always consider this condition as one of the causes of shock or cardiopulmonary arrest, even without an apparent medical history of active air infusion or trauma.
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http://dx.doi.org/10.1186/s13256-020-02437-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374859PMC
July 2020

Administration of recombinant human placental growth factor decreases blood pressure in obese hypertensive pregnant rats.

J Hypertens 2020 Nov;38(11):2295-2304

Department of Surgery, Cardiovascular-Renal Research Center, University of Mississippi Medical Center, Jackson, Mississippi.

Objectives: Although epidemiological studies have shown that obesity is associated with increased incidence of hypertension during pregnancy, the mechanisms linking these two comorbidities are not as well studied. Previous investigations detected lower levels of the anti-hypertensive and pregnancy-related factor, placental growth factor (PlGF), in obese hypertensive pregnancies. Therefore, we examined whether obese hypertensive pregnant rats have reduced PlGF and whether increasing its levels by administering recombinant human (rh)PlGF reduces their blood pressure.

Methods: We utilized a genetic model of obesity characterized to be heavier, hypertensive and fertile, namely rats having heterozygous deficiency of the melanocortin-4 receptor (MC4R-def).

Results: MC4R-def obese rats had lower circulating levels of PlGF than wild-type lean controls at gestational day 19. Also, assessment of the PlGF receptor, Flt-1, in the vasculature showed that its levels were reduced in aorta and kidney glomeruli but increased in small mesenteric arteries. Chronic intraperitoneal administration of rhPlGF from gestational day 13-19 significantly increased circulating PlGF levels in both obese and lean rats, but reduced blood pressure only in the obese pregnant group. The rhPlGF treatment did not alter maternal body and fat masses or circulating levels of the adipokines, leptin and adiponectin. In addition, this treatment did not impact average foetal weights but increased placental weights regardless of obese or lean pregnancy.

Conclusion: PlGF is reduced in MC4R-def obese hypertensive pregnant rats, which is similar to findings in obese hypertensive pregnant women, while increasing its levels with exogenous rhPlGF reduces their blood pressure.
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http://dx.doi.org/10.1097/HJH.0000000000002528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544658PMC
November 2020

The association between arterial pulse waveform analysis device and in-hospital mortality in high-risk non-cardiac surgeries.

Acta Anaesthesiol Scand 2020 08 14;64(7):928-935. Epub 2020 Apr 14.

Department of Pharmacoepidemiology, Graduate School of Medicine and Public Health, Kyoto University, Kyoto, Japan.

Background: Perioperative goal-directed fluid therapy is used for haemodynamic optimization in high-risk surgeries. Cardiac output monitoring can be performed by a specialized pressure transducer for arterial pulse waveform analysis (S-APWA). No study has assessed whether real-world use of S-APWA is associated with post-operative outcomes; therefore, using a Japanese administrative claims database, we retrospectively investigated whether S-APWA use is associated with in-hospital mortality among patients undergoing high-risk surgery under general anaesthesia.

Methods: Adult patients who underwent high-risk surgery under general anaesthesia and arterial catheterization between 2014 and 2016 were divided into S-APWA and conventional arterial pressure transducer groups, then compared regarding baseline factors and outcomes. Logistic regression analysis was performed to compare in-hospital mortality. Subgroup analyses evaluated S-APWA efficacy and outcomes based on the type of surgery and patients' comorbidity.

Results: S-APWA was used in 6859 of 23 655 (29.0%) patients; the crude in-hospital mortality rate was 3.5%. Adjusted analysis showed no significant association between S-APWA use and in-hospital mortality rate (adjusted odds ratio [aOR] = 0.91; 95% confidence interval [CI]: 0.76-1.07; P = .25). S-APWA use was associated with significantly lower in-hospital mortality in patients undergoing vascular surgery (aOR = 0.67; 95% CI: 0.49-0.94), and significantly higher in-hospital mortality in patients undergoing lower limb amputation (aOR = 2.63; 95% CI: 1.32-5.22). S-APWA use and in-hospital mortality were not significantly associated with other subgroups.

Conclusion: S-APWA use was not associated with in-hospital mortality in the entire study population. However, S-APWA was associated with decreased in-hospital mortality among vascular surgery and increased in-hospital mortality among lower limb amputation.
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http://dx.doi.org/10.1111/aas.13584DOI Listing
August 2020

CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, accelerates Phytophthora elicitin-mediated defense response.

Biochem Biophys Res Commun 2020 04 12;524(4):951-956. Epub 2020 Feb 12.

Department of Life Sciences, Korea University, Seoul, 02841, Republic of Korea. Electronic address:

Innate immunity in plants relies on the recognition of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) located on the plant cell surface. CaLecRK-S.5, a pepper L-type lectin receptor kinase, has been shown to confer broad-spectrum resistance through priming activation. To further elucidate the molecular mechanism of CaLecRK-S.5, transgenic tobacco plants were generated in this study. Interestingly, hemizygous transgenic plants exhibited a high accumulation of CaLecRK-S.5, but this accumulation was completely abolished in homozygous transgenic plants by a cosuppression mechanism. Gain-of-function and loss-of-function analyses revealed that CaLecRK-S.5 plays a positive role in Phytophthora elicitin-mediated defense responses.
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http://dx.doi.org/10.1016/j.bbrc.2020.02.014DOI Listing
April 2020

A new droplet breakup phenomenon in electrokinetic flow through a microchannel constriction.

Electrophoresis 2020 06 14;41(10-11):758-760. Epub 2019 Jun 14.

School of Mechanical Engineering, Yeungnam University, Gyongsan, South Korea.

A completely new droplet breakup phenomenon is reported for droplets passing through a constriction in an electrokinetic flow. The breakup occurs during the droplet shape recovery process past the constriction throat by the interplay of the dielectrophoretic stress release and the interface energy for droplets with smaller permittivity than that of the ambient fluid. There are conditions for constriction ratios and droplet size that the droplet breakup occurs. The numerical predictions provided here require experimental verification, and then can give rise to a novel microfluidic device design with novel droplet manipulations.
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http://dx.doi.org/10.1002/elps.201900140DOI Listing
June 2020

Temperature-dependent increase in the calcium sensitivity and acceleration of activation of ANO6 chloride channel variants.

Sci Rep 2019 04 30;9(1):6706. Epub 2019 Apr 30.

Department of Physiology, Dongguk University College of Medicine, 123 Dongdae-ro, Gyeongju, 38066, Republic of Korea.

Anoctamin-6 (ANO6) belongs to a family of calcium (Ca)-activated chloride channels (CaCCs), with three splicing variants (V1, V2, and V5) showing plasma membrane expression. Unlike other CaCCs, ANO6 requires a non-physiological intracellular free calcium concentration ([Ca] > 1 μM) and several minutes for full activation under a whole-cell patch clamp. Therefore, its physiological role as an ion channel is uncertain and it is more commonly considered a Ca-dependent phospholipid scramblase. Here, we demonstrate that physiological temperature (37 °C) increases ANO6 Ca sensitivity under a whole-cell patch clamp; V1 was activated by 1 μM [Ca], whereas V2 and V5 were activated by 300 nM [Ca]. Increasing the temperature to 42 °C led to activation of all ANO6 variants by 100 nM [Ca]. The delay tme for activation of the three variants was significantly shortened at 37 °C. Notably, the temperature-dependent Ca-sensitisation of ANO6 became insignificant under inside-out patch clamp, suggesting critical roles of unknown cytosolic factors. Unlike channel activity, 27 °C but not 37 °C (physiological temperature) induced the scramblase activity of ANO6 at submicromolar [Ca] (300 nM), irrespective of variant type. Our results reveal a physiological ion conducting property of ANO6 at 37 °C and suggest that ANO6 channel function acts separately from its scramblase activity.
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http://dx.doi.org/10.1038/s41598-019-43162-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491614PMC
April 2019

Effectiveness and safety of early enteral nutrition for patients who received targeted temperature management after out-of-hospital cardiac arrest.

Resuscitation 2019 02 14;135:191-196. Epub 2019 Jan 14.

Department of Pharmacoepidemiology, Graduate School of Medicine and Public Health, Kyoto University, Yoshidakonoecho, Sakyo-ku, Kyoto-city, Japan; Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Kyoto, Japan. Electronic address:

Aim: Early enteral nutrition (EN) is recommended for critically ill patients; however, few reports have examined early EN for patients who received targeted temperature management (TTM) after out-of-hospital cardiac arrest (OHCA). We investigated the effectiveness and safety of early EN for patients who received TTM after OHCA.

Methods: We used a nationwide Japanese administrative database to identify OHCA patients who received TTM from April 2008 to March 2017. The primary outcome was 30-day mortality; secondary outcomes were incidences of all-cause infection, pneumonia, and intestinal ischemia.

Results: Of the 1932 OHCA patients who received TTM, 1682 met the inclusion criteria. Of these, 294 received early EN within 2 days from the initiation of TTM and 266 propensity-score matched pairs were generated. Cox regression analyses revealed no significant difference in 30-day mortality between groups (hazard ratio (HR): 0.90; 95% confidence interval (95% CI): 0.65-1.25). There was no significant difference in the incidence of all-cause infection (odds ratio (OR): 0.98; 95% CI: 0.66-1.46) or pneumonia (OR: 1.02; 95% CI: 0.68-1.55). Subgroup analyses of patients with a low body mass index (BMI; kg/m2) (< 18.5) revealed a significant decrease of 30-day mortality in the early EN group (HR: 0.30; 95% CI: 0.092-0.97) but no significant difference among patients with a BMI ≥ 18.5 (HR: 1.01; 95% CI: 0.72-1.43).

Conclusion: Among patients who received TTM after OHCA, there was no significant association between early EN and 30-day mortality; however, early EN could be beneficial for patients with a low BMI.
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http://dx.doi.org/10.1016/j.resuscitation.2019.01.007DOI Listing
February 2019

Utilization of arterial pulse waveform analysis during non-cardiac surgery in Japan: a retrospective observational study using a nationwide claims database.

J Anesth 2019 02 7;33(1):159-162. Epub 2019 Jan 7.

Department of Pharmacoepidemiology, Graduate School of Medicine and Public Health, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan.

Arterial pulse waveform analysis (APWA) is used for cardiac output monitoring. However, data on the frequency of and patient characteristics for specialized pressure transducer for APWA (S-APWA) use are lacking. We retrospectively identified 175,201 patients aged 18 years or older, who underwent non-cardiac surgery under general anesthesia with an arterial catheter from January 1, 2014, to December 31, 2016. We extracted data on patient demographics, comorbidities, surgical and anesthesia characteristics, and hospital characteristics. Among the full study cohort, 24,605 (14.0%) patients were monitored using S-APWA. Further, the use of S-APWA was higher in patients undergoing high-risk surgery than in those undergoing low-risk surgery [high vs low: adjusted odds ratio (aOR) 1.95; 95% confidence interval (CI) 1.76-2.15, moderate vs low: aOR 1.11; 95% CI 1.01-1.22] and those with more comorbidities than in those with less comorbidities (high vs low: aOR 1.49; 95% CI 1.42-1.56, moderate vs low: aOR 1.25; 95% CI 1.20-1.31). S-APWA use was significantly associated with both surgery risk and patients' comorbidities. In conclusion, our study may provide a benchmark for future studies related to the appropriate use of S-APWA.
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http://dx.doi.org/10.1007/s00540-018-02609-xDOI Listing
February 2019

Nasal Stenosis Treated With a Composite Skin Graft.

J Craniofac Surg 2019 Jan;30(1):e9-e10

Department of Otolaryngology-Head and Neck Surgery, Gil Medical Center, Gachon University School of Medicine, Incheon.

A 58-year-old female patient was presented, who complained about breathing and aesthetic difficulties due to external nasal valve obstruction and nasal deformity that developed after nasal trauma surgery. Nasal stenosis recurs easily after surgery, especially if internal nasal stenosis is not adequately managed. Nasal stenosis in this case was well treated using a composite skin graft.
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http://dx.doi.org/10.1097/SCS.0000000000004851DOI Listing
January 2019

Retained Eyeglasses in the Orbital Cavity After Penetrating Facial Trauma.

J Craniofac Surg 2018 Oct;29(7):e679-e680

Department of Otolaryngology-Head and Neck Surgery.

This study describes a 68-year-old man who was presented to the emergency department with left orbital cavity penetration by his eyeglasses. The eyeglasses had entered the orbit and at presentation his eyesight could not be measured. The foreign body was extracted carefully and panfacial bone fractures were then reduced through lateral rhinotomy combined with a lip-splitting incision. When planning removal of an unusual foreign body from the orbital cavity, care should be taken not to injure the optic nerve, periorbital musculatures, or the eyeball.
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http://dx.doi.org/10.1097/SCS.0000000000004854DOI Listing
October 2018

Frontal Sinus Osteoplastic Flap Surgery Using a Surgical Navigation System.

J Craniofac Surg 2018 Oct;29(7):e662-e663

Department of Otolaryngology-Head and Neck Surgery, Gil Medical Center, Gachon University School of Medicine, Incheon.

Objective: To report the case of a 38-year-old woman who underwent osteoplastic flap surgery for recurrent frontal sinus mucocele. During surgery, the exact shape of the frontal sinus was duplicated using a surgical navigation system.

Methods: In this case report, the authors suggest intraoperative surgical navigation systems are useful for accurately determining the dimensions of the frontal sinus for osteoplastic flap surgery.

Results: The patient underwent successful and safe osteoplastic flap surgery using a surgical navigation system.

Conclusion: Surgical navigation is useful and safe for frontal sinus osteoplastic flap surgery.
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http://dx.doi.org/10.1097/SCS.0000000000004729DOI Listing
October 2018

TMEM16F/ANO6, a Ca-activated anion channel, is negatively regulated by the actin cytoskeleton and intracellular MgATP.

Biochem Biophys Res Commun 2018 09 10;503(4):2348-2354. Epub 2018 Jul 10.

Department of Physiology, Dongguk University College of Medicine, 123 Dongdae-ro, Gyeongju, 38066, Republic of Korea; Channelopathy Research Center (CRC), Dongguk University College of Medicine, 32 Dongguk-ro, Ilsan Dong-gu, Goyang, Gyeonggi-do, 10326, Republic of Korea. Electronic address:

Anoctamin 6 (ANO6/TMEM16F) is a recently identified membrane protein that has both phospholipid scramblase activity and anion channel function activated by relatively high [Ca]. In addition to the low sensitivity to Ca, the activation of ANO6 Cl conductance is very slow (>3-5 min to reach peak level at 10 μM [Ca]), with subsequent inactivation. In a whole-cell patch clamp recording of ANO6 current (I), disruption of the actin cytoskeleton with cytochalasin-D (cytoD) significantly accelerated the activation kinetics, while actin filament-stabilizing agents (phalloidin and jasplakinolide) commonly inhibited I. Inside-out patch clamp recording of ANO6 (I) showed immediate activation by raising [Ca]. We also found that intracellular ATP (3 mM MgATP in pipette solution) decelerated the activation of I, and also prevented the inactivation of I. However, the addition of cytoD still accelerated both activation and inactivation of I. We conclude that the actin cytoskeleton and intracellular ATP play major roles in the Ca-dependent activation and inactivation of I, respectively.
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http://dx.doi.org/10.1016/j.bbrc.2018.06.160DOI Listing
September 2018

Wireless mobile ultrasonography-assisted parotid duct stone removal.

Ear Nose Throat J 2018 Apr-May;97(4-5):E36-E38

Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, Gachon University, Gil Medical Center, 1189, Guwol-dong, Namdong-gu, Incheon, Republic of Korea.

Ultrasonography is highly sensitive for the diagnosis of sialoliths. Recently, wireless mobile ultrasonography was developed. We describe the case of a 49-year-old man who presented with painful postprandial left cheek swelling. Computed tomography detected a solitary 5-mm parotid duct stone with infection at the anterior portion of the left masseter muscle. Transoral stone removal was planned, although difficulty was expected in view of the surrounding infection. Surgery was performed under the guidance of mobile ultrasonography, and the stone was removed safely.
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http://dx.doi.org/10.1177/0145561318097004-503DOI Listing
November 2018

Judicial Precedent-Based Clinical Practice Guidelines of Propofol in Sedative Esthetic Surgery.

Aesthetic Plast Surg 2018 Jun 2;42(3):891-898. Epub 2018 Apr 2.

Department of Plastic Surgery, College of Medicine, Ewha Womans University Mokdong Hospital, Seoul, South Korea.

Background: Propofol is has been widely used for sedation in the field of esthetic surgery because of its favorable pharmacokinetic profile. Propofol sedation-induced side effects are rare. However, when present, they can be serious. The number of malpractice claims associated with propofol sedation has increased in recent years. This study aims to show which procedures lead to the most claims in the field of esthetic surgery through a review of Korean precedents.

Methods: Thirteen precedent cases of propofol sedation in the field of esthetic surgery were collected between 2000 and 2016. We analyzed the type of procedure, administration route, anesthesia provider, complications, timing of damaging events, average indemnification, plaintiff's (patients) winning rate, ratio and the reason of limitation of liability and the key factors affecting the judgement in these cases.

Results: Most plaintiffs were women, and in most cases (11/13, 73.3%), the times of the damaging events were in maintenance and the anesthesia provider was the surgeon. The most common complication related to propofol sedation was hypoxic brain damage. Among the 13 cases, 12 were won by the plaintiff. The mean claim settlement was 339,455,814 KRW (USD 301,792.15). The key factors affecting the judgement were administration method and staff, monitoring method, preparation of emergency kit, response to emergencies, transfer to a higher-level hospital, detailed medical recording about event and informed consent.

Conclusion: The number of claims owing to propofol sedation after esthetic surgery is increasing. Close monitoring during the operation, immediate reaction to an event and thorough medical records were main key factors that influenced the judgement. Preoperative explanation about the possibility of complications was important. The findings will help surgeons achieve high patient satisfaction and reduce liability concerns.

Level Of Evidence V: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-018-1122-1DOI Listing
June 2018

Superior peroxidase mimetic activity of tungsten disulfide nanosheets/silver nanoclusters composite: Colorimetric, fluorometric and electrochemical studies.

J Colloid Interface Sci 2018 Apr 4;515:39-49. Epub 2018 Jan 4.

School of Mechanical Engineering, Yeungnam University, Gyeongsan 712-749 South Korea. Electronic address:

Developing a novel peroxidase nano-mimetic is a challenging research topic in biosensing field. Herein, WS nanosheets (WS NS) decorated with silver nanoclusters (AgNCs) was introduced as a new nanocomposite with improved peroxidase mimetic behavior. WS NS/AgNCs nanocomposite was synthesized by simple chemical reduction of silver cations in the presence of WS NS. The enhanced catalytic activity of nanocomposite in chemical and electrochemical reduction of HO was studied using colorimetry, fluorometry and electrochemical techniques. Attaching the AgNCs on the surface of WS NS effectively improved the catalytic activity of these nanosheets, which may be connected to the difference of the Fermi energy levels of coupled nanomaterial. The unequal Fermi levels cause charge separation between two phases creating highly active sites on the interface of coupled nanomaterial. Moreover, the new mimetic nanocomposite was applied for the analysis of glucose in blood, based on its enzymatic oxidation using glucose oxidase and then, on the measurement of produced HO by sensitive fluorescence detection system. In optimum condition, a linear association was found between the generated fluorescence intensity and glucose logarithmic concentration in the range of 0.05-400 µM, and the limit of detection (3S/m) was 21 nM.
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http://dx.doi.org/10.1016/j.jcis.2018.01.013DOI Listing
April 2018

Dielectrophoretic choking phenomenon of a deformable particle in a converging-diverging microchannel.

Electrophoresis 2018 02 27;39(4):590-596. Epub 2017 Dec 27.

School of Mechanical Engineering, Yeungnam University, Gyongsan, Korea.

The translational motion of small particles in an electrokinetic fluid flow through a constriction can be enhanced by an increase of the applied electric potential. Beyond a critical potential, however, the negative dielectrophoresis (DEP) can overpower other forces to prevent particles that are even smaller than the constriction from passing through the constriction. This DEP choking phenomenon was studied previously for rigid particles. Here, the DEP choking phenomenon is revisited for deformable particles, which are ubiquitous in many biomedical applications. Particle deformability is measured by the particle shear modulus, and the choking conditions are reported through a parametric study that includes the channel geometry, external electric potential, and particle zeta potential. The study was carried out using a numerical model based on an arbitrary Lagrangian-Eulerican (ALE) finite-element method.
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http://dx.doi.org/10.1002/elps.201700250DOI Listing
February 2018

The Effect of Human Adipose Tissue-Derived Mesenchymal Stem Cells in Rat's Subglottic Stenosis Model.

Ann Otol Rhinol Laryngol 2018 Jan 29;127(1):5-12. Epub 2017 Nov 29.

2 Department of Otolaryngology-Head and Neck Surgery, Gachon University, Gil Medical Center, Incheon, Republic of Korea.

Objective: Subglottic stenosis remains a clinical challenge. The aim of this study was to evaluate the effect of human adipose tissue-derived mesenchymal stem cells (hAMSCs) in rat model of subglottic stenosis.

Subjects And Methods: Ninety-six 13-week-old male rats were enrolled in this study. They were divided into 3 groups as normal control (NC) group, a subglottic injury and media injection (SM) group, and a subglottic injury and media-stem cell injection (SMSC) group. The hAMSCs were immediately injected into subglottis after injury. Histologic characteristics of subglottis; the mRNA expressions of interleukin-1β, cyclooxygenase-2, tumor growth factor-β and basic fibroblast growth factor; and hAMSCs' survival were evaluated.

Results: The hAMSCs survived in the subglottis of the rat until 10 days after implantation. The NC and SMSC groups had a significantly wider subglottic lumen and thinner lamina propria than the SM group at 56 days after injury. Collagen intensity of subglottis was significantly higher in the SM group than in the NC and SMSC groups at 28 days after injury. Gene expression didn't show significant difference between the SM group and the SMSC group.

Conclusions: The hAMSCs injection was found to be helpful for preventing subglottic stenosis in a rat model.
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http://dx.doi.org/10.1177/0003489417739920DOI Listing
January 2018

Macrophage Response to Allogeneic Adipose Tissue-Derived Stromal Cells in Hyaluronan-Based Hydrogel in a Porcine Vocal Fold Injury Model.

Ann Otol Rhinol Laryngol 2017 Jun 6;126(6):463-477. Epub 2017 Apr 6.

3 Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Objective: Adipose tissue-derived stromal cells (ASC) embedded in hyaluronan scaffold is a beneficial prophylactic treatment for vocal fold (VF) surgical scar. Here, we investigated the macrophage inflammatory response to allogeneic ASC-constructs and identified changes in lamina propria extracellular matrix.

Method: Pig ASC were characterized and transfected with GFP+ lentivirus. Thirty-three pigs underwent VF biopsies, and after 3 days, gel alone, gel+pASC, placebo, or pASC alone was injected into wound bed. Animals were sacrificed 3, 7, or 26 days post-injection. Flow cytometry; qPCR for NF-α, TGFβ, IL-10, IL-4, IFNγ, IL-12, FGF2, Col1A1, and HGF; and immunohistochemistry for collagen, elastin, HA, and fibronectin were performed to characterize macrophage phenotype, quantify cytokine transcription, analyze extracellular matrix remodeling, and track GFP+ cells.

Results: No significant differences were found in SWC3+/SWC9+ phenotype or mRNA expression between cells+gel, gel, or placebo. The ASC alone exhibited significantly greater collagen, gel alone resulted in significantly less hyaluronan, and gel+pASC significantly more fibronectin (all P < .05). The pASC-GFP+ were detected 26 days post-injection.

Conclusions: The ASC-constructs were biocompatible; they did not influence the macrophage inflammatory response or provoke increases in collagen expression. Long-term engraftment was confirmed.
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http://dx.doi.org/10.1177/0003489417702923DOI Listing
June 2017

CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, confers broad-spectrum resistance by activating priming.

J Exp Bot 2016 10 19;67(19):5725-5741. Epub 2016 Sep 19.

Department of Life Sciences, Korea University, Seoul 02841, Republic of Korea

In Arabidopsis, several L-type lectin receptor kinases (LecRKs) have been identified as putative immune receptors. However, to date, there have been few analyses of LecRKs in crop plants. Virus-induced gene silencing of CaLecRK-S.5 verified the role of CaLecRK-S.5 in broad-spectrum resistance. Compared with control plants, CaLecRK-S.5-silenced plants showed reduced hypersensitive response, reactive oxygen species burst, secondary metabolite production, mitogen-activated protein kinase activation, and defense-related gene expression in response to Tobacco mosaic virus pathotype P (TMV-P) infection. Suppression of CaLecRK-S.5 expression significantly enhanced the susceptibility to Pepper mild mottle virus pathotype P, Xanthomonas campestris pv. vesicatoria, Phytophthora capsici, as well as TMV-P Additionally, β-aminobutyric acid treatment and a systemic acquired resistance assay revealed that CaLecRK-S.5 is involved in priming of plant immunity. Pre-treatment with β-aminobutyric acid before viral infection restored the reduced disease resistance phenotypes shown in CaLecRK-S.5-silenced plants. Systemic acquired resistance was also abolished in CaLecRK-S.5-silenced plants. Finally, RNA sequencing analysis indicated that CaLecRK-S.5 positively regulates plant immunity at the transcriptional level. Altogether, these results suggest that CaLecRK-S.5-mediated broad-spectrum resistance is associated with the regulation of priming.
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http://dx.doi.org/10.1093/jxb/erw336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066492PMC
October 2016

Small heterodimer partner SHP mediates liver X receptor (LXR)-dependent suppression of inflammatory signaling by promoting LXR SUMOylation specifically in astrocytes.

Sci Signal 2016 08 2;9(439):ra78. Epub 2016 Aug 2.

Department of Pharmacology, Chronic Inflammatory Disease Research Center, Ajou University School of Medicine, Suwon 442-721, Korea.

Liver X receptors (LXRs) suppress the expression of inflammatory genes in a context-specific manner. In astrocytes, SUMOylation of LXRs promotes their anti-inflammatory effects. We found that small heterodimer partner (SHP), also known as NR0B2 (nuclear receptor subfamily 0, group B, member 2), facilitates the anti-inflammatory actions of LXRs by promoting their SUMOylation. Knockdown of SHP abrogated SUMOylation of LXRs, preventing their anti-inflammatory effects, in primary rat astrocytes but not macrophages. The underlying mechanisms differed according to LXR isoform. SHP promoted SUMO2 and SUMO3 attachment to LXRα by interacting directly with the histone deacetylase and E3 SUMO ligase HDAC4. In contrast, SHP promoted SUMO1 attachment to LXRβ by stabilizing the E3 SUMO ligase PIAS1. SHP bound PIAS1 and disrupted its interaction with the E3 ubiquitin ligase SIAH1. Knocking down SIAH1 rescued LXRβ SUMOylation in SHP-deficient astrocytes. Our data collectively suggested that SHP mediates the anti-inflammatory actions of LXRs through differential regulation of receptor SUMOylation specifically in astrocytes, thereby revealing potential avenues for therapeutic development in diseases associated with brain inflammation.
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http://dx.doi.org/10.1126/scisignal.aaf4850DOI Listing
August 2016

22(R)-hydroxycholesterol induces HuR-dependent MAP kinase phosphatase-1 expression via mGluR5-mediated Ca(2+)/PKCα signaling.

Biochim Biophys Acta 2016 08 17;1859(8):1056-70. Epub 2016 May 17.

Department of Pharmacology and Chronic Inflammatory Disease Research Center, Ajou University School of Medicine, Suwon 16499, Korea. Electronic address:

MAP kinase phosphatase (MKP)-1 plays a pivotal role in controlling MAP kinase (MAPK)-dependent (patho) physiological processes. Although MKP-1 gene expression is tightly regulated at multiple levels, the underlying mechanistic details remain largely unknown. In this study, we demonstrate that MKP-1 expression is regulated at the post-transcriptional level by 22(R)-hydroxycholesterol [22(R)-HC] through a novel mechanism. 22(R)-HC induces Hu antigen R (HuR) phosphorylation, cytoplasmic translocation and binding to MKP-1 mRNA, resulting in stabilization of MKP-1 mRNA. The resulting increase in MKP-1 leads to suppression of JNK-mediated inflammatory responses in brain astrocytes. We further demonstrate that 22(R)-HC-induced phosphorylation of nuclear HuR is mediated by PKCα, which is activated in the cytosol by increases in intracellular Ca(2+) levels mediated by the phospholipase C/inositol 1,4,5-triphosphate receptor (PLC/IP3R) pathway and translocates from cytoplasm to nucleus. In addition, pharmacological interventions reveal that metabotropic glutamate receptor5 (mGluR5) is responsible for the increases in intracellular Ca(2+) that underlie these actions of 22(R)-HC. Collectively, our findings identify a novel anti-inflammatory mechanism of 22(R)-HC, which acts through PKCα-mediated cytoplasmic shuttling of HuR to post-transcriptionally regulate MKP-1 expression. These findings provide an experimental basis for the development of a RNA-targeted therapeutic agent to control MAPK-dependent inflammatory responses.
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http://dx.doi.org/10.1016/j.bbagrm.2016.05.008DOI Listing
August 2016

MERS versus Standard Surgical Approaches for Porcine Vocal Fold Scarring with Adipose Stem Cell Constructs.

Otolaryngol Head Neck Surg 2016 10 10;155(4):612-23. Epub 2016 May 10.

Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, USA

Objective: Cells, scaffold, and surgical approaches are important for regeneration of the lamina propria of the scarred vocal fold (VF). Microendoscopy of Reinke's space (MERS) is a surgical approach used to access the lamina propria. The present study evaluated MERS in the treatment of VF scarring as compared with standardized approaches for the treatment of VF scarring with adipose stem cell constructs.

Study Design: Animal study.

Setting: Academic center.

Subjects And Methods: VF injury was performed bilaterally to induce scarring in 20 pigs. Eight weeks after injury, pigs were classified into no treatment, minithyrotomy, VF injection, VF incision/dissection, and MERS. All groups (except control) were implanted with adipose stem cell and hyaluronan. Four weeks after treatment, histology for collagen, hyaluronan, and fibronectin; mRNA expression for α-smooth muscle actin, tumor growth factor β1, collagen 1α1, collagen 3α1, matrix metalloproteinase 2, basic fibroblast growth factor, and hepatocyte growth factor; and tissue rheology were evaluated.

Results: Differences were measured among surgical approaches for protein levels of collagen, hyaluronan, and fibronectin (P = .0133, P < .0001, and P = .0025, respectively). Fibroblast growth factor, collagen 1α1, and matrix metalloproteinase 2 transcript levels were different among treatment groups (P = .003, P = .0086, and P = .014, respectively), while no differences were measured for α-smooth muscle actin, tumor growth factor β1, hepatocyte growth factor, and collagen 3α1. Rheologically, significant differences were not measured between groups.

Conclusion: MERS is a promising surgical approach for the treatment of VF scarring, optimizing the placement of implanted biomaterials.
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http://dx.doi.org/10.1177/0194599816645772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7032050PMC
October 2016

PINK1 Deficiency Decreases Expression Levels of mir-326, mir-330, and mir-3099 during Brain Development and Neural Stem Cell Differentiation.

Exp Neurobiol 2016 Feb 22;25(1):14-23. Epub 2016 Feb 22.

Neuroscience Graduate Program Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 16499, Korea.; Chronic Inflammatory Disease Research Center, Ajou University School of Medicine, Suwon 16499, Korea.; Department of Pharmacology, Ajou University School of Medicine, Suwon 16499, Korea.; Department of Brain Science, Ajou University School of Medicine, Suwon 16499, Korea.; Brain Disease Research Center, Ajou University School of Medicine, Suwon 16499, Korea.

PTEN-induced putative kinase 1 (PINK1) is a Parkinson's disease (PD) gene. We examined miRNAs regulated by PINK1 during brain development and neural stem cell (NSC) differentiation, and found that lvels of miRNAs related to tumors and inflammation were different between 1-day-old-wild type (WT) and PINK1-knockout (KO) mouse brains. Notably, levels of miR-326, miR-330 and miR-3099, which are related to astroglioma, increased during brain development and NSC differentiation, and were significantly reduced in the absence of PINK1. Interestingly, in the presence of ciliary neurotrophic factor (CNTF), which pushes differentiation of NSCs into astrocytes, miR-326, miR-330, and miR-3099 levels in KO NSCs were also lower than those in WT NSCs. Furthermore, mimics of all three miRNAs increased expression of the astrocytic marker glial fibrillary acidic protein (GFAP) during differentiation of KO NSCs, but inhibitors of these miRNAs decreased GFAP expression in WT NSCs. Moreover, these miRNAs increased the translational efficacy of GFAP through the 3'-UTR of GFAP mRNA. Taken together, these results suggest that PINK1 deficiency reduce expression levels of miR-326, miR-330 and miR-3099, which may regulate GFAP expression during NSC differentiation and brain development.
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http://dx.doi.org/10.5607/en.2016.25.1.14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766110PMC
February 2016

Erratum to: PINK1 expression increases during brain development and stem cell differentiation, and affects the development of GFAPpositive astrocytes.

Mol Brain 2016 Feb 15;9:18. Epub 2016 Feb 15.

Neuroscience Graduate Program Department of Biomedical Sciences, Ajou University School of Medicine, Suwon, Korea.

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http://dx.doi.org/10.1186/s13041-016-0199-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753649PMC
February 2016