Publications by authors named "Won Hur"

27 Publications

  • Page 1 of 1

Complete Genome Sequence of Mycoplasma gallisepticum Strain KUVMG001, an Isolate from South Korea.

Microbiol Resour Announc 2021 May 13;10(19). Epub 2021 May 13.

College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea

causes chronic respiratory diseases in poultry and economic losses to the chicken and turkey industry. We report the complete genome sequence of the field isolate strain KUVMG001 of from South Korea.
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http://dx.doi.org/10.1128/MRA.00331-21DOI Listing
May 2021

PVP-stabilized PtRu nanozymes with peroxidase-like activity and its application for colorimetric and fluorometric glucose detection.

Colloids Surf B Biointerfaces 2021 Apr 24;204:111783. Epub 2021 Apr 24.

Department of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan, 426-791, South Korea. Electronic address:

Nanozymes have significant advantages over natural enzymes. The intrinsic peroxidase-like activity of Pt-based nanomaterials can be enhanced by alloying with other transition metals, such as Ru, that have great catalytic activity. In this study, we used polyvinylpyrrolidone (PVP) to synthesize well-dispersed and homogeneous nanostructures. PVP-stabilized Pt-Ru nanozymes (PVP/PtRu NZs) were synthesized and characterized. The PVP/PtRu NZs had an average size of 3.54 ± 0.84 nm and exhibited an intense peroxidase-like activity. The PVP/PtRu NZs were used as peroxidase mimics for colorimetric and fluorometric glucose determination by the glucose oxidase and PVP/PtRu NZs cascade reaction. In the colorimetric assay, the linearly detectable range was 0.25-3.0 mM, with an R and limit of detection (LOD) of 0.988 and 138 μM, respectively. In the fluorometric assay, a linear relationship was found when the glucose concentration was between 5.0 and 300 μM (R = 0.997), with an LOD of 1.11 μM. Compared to the colorimetric assay, the fluorometric assay had greater sensitivity and a lower detection limit for the determination of glucose. Moreover, the PVP/PtRu NZs had high storage stability over a month and great recovery values in human serum and artificial urine, with a range of 94-106 %. From these results, PVP/PtRu NZs are expected to be used as promising peroxidase mimics in various fields such as biosensing, pharmaceutical processing, and the food industry.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111783DOI Listing
April 2021

Cell-based electrochemical cytosensor for rapid and sensitive evaluation of the anticancer effects of saponin on human malignant melanoma cells.

Bioelectrochemistry 2021 Mar 31;140:107813. Epub 2021 Mar 31.

Department of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 426-791, South Korea. Electronic address:

Discovering new anticancer agents and analyzing their activities is a vital part of drug development, but it requires a huge amount of time and resources, leading to the increasing demands for more-effective techniques. Herein, a novel and simple cell-based electrochemical biosensor, referred to as a cytosensor, was proposed to investigate the electrochemical behavior of human skin malignant melanoma (SK-MEL28) cells and the anticancer effect of saponin on cell viability. To enhance both electrocatalytic properties and biocompatibility, gold nanoparticles were electrochemically deposited onto a conductive substrate, and poly-L-lysine was further added to the electrode surface. Electric signals from SK-MEL28 cells on the electrodes were obtained from cyclic voltammetry and differential pulse voltammetry. The cathodic peak current was proportional to the cell viability and showed a detection range of 2,880-40,000 cells per device with an excellent linear cell number-intensity relationship (R= 0.9952). Furthermore, the anticancer effect of saponin on SK-MEL28 cells was clearly established at concentrations higher than 20 μM, which was highly consistent with conventional assays. Moreover, the developed electrochemical cytosensor for evaluating anticancer effects enabled rapid (<2 min), sensitive (LOQ: 2,880cells/device), and non-invasive measurements, thus providing a new avenue for assessing the anticancer drugs in vitro.
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http://dx.doi.org/10.1016/j.bioelechem.2021.107813DOI Listing
March 2021

Determination of glycated albumin using a Prussian blue nanozyme-based boronate affinity sandwich assay.

Anal Chim Acta 2020 Oct 20;1134:41-49. Epub 2020 Aug 20.

Department of Bionano Engineering, Hanyang University, Ansan, 426-791, South Korea. Electronic address:

Nanozymes are effective substitutes for natural enzymes and offer multiple advantages. Here, synthesized Prussian blue nanoparticles (PBNPs) exhibited excellent peroxidase-like activity, catalyzing the oxidation of 3,5,3',5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide within 1 min. Oxidized TMB (TMB) underwent a color change from transparent to blue and then yellow by a stop solution. Moreover, the TMB could be reduced on an indium tin oxide electrode, generating an electrochemical current, indicating that TMB can be used as a colorimetric and electrochemical indicator. The PBNPs modified with 3-aminophenylboronic acid (APBA) captured glycated albumin (GA) with a boronate affinity sandwich assay. As boronic acid binds to glycoproteins using cis-diol bonding, it can be used to detect GA. The APBA-modified PBNPs (PBBA) were involved with a sandwich complex formation and employed as nanozymes for the quantitative analysis of GA using colorimetric and electrochemical methods. Both methods showed strong linearities for different concentrations of GA. The results show that PBBA is a suitable alternative for natural enzymes and can be applied to sensitive determination of GA.
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http://dx.doi.org/10.1016/j.aca.2020.08.015DOI Listing
October 2020

Maintenance of viability and proliferation of 3T3 cell aggregates incorporating fibroin microspheres into cultures.

Cytotechnology 2020 Aug 14;72(4):579-587. Epub 2020 Aug 14.

Department of Biotechnology and Bioengineering, Kangwon National University, Chuncheon, 200-701, South Korea.

This study investigated whether micron-sized microspheres can be used as dispersed scaffolds where anchorage-dependent cells can proliferate and survive in suspension culture. Aggregates of murine 3T3 cells in a non-adherent plate cultured remained viable for more than 2 weeks by the presence of 0.5 mg/ml fibroin microspheres. A nucleoside incorporation assay confirmed the proliferation of 3T3 cells in the aggregates only when cultured with microspheres. Under these conditions, the glucose consumption rate of 3T3 cells increased to 66.5 nmol day cell. Histological analysis demonstrated that the intercellular space of cell aggregates was larger in cultures supplemented with 0.5 mg/ml microspheres than in non-supplemented cultures. The cell aggregates with microspheres also exhibited a reduced arrest in G1 phase. Transmission electron microscopy verified the presence of microspheres in the space between cells in aggregates. Fibroin microspheres maintained the viability and proliferability of 3T3 cells cultured under non-adherent conditions and thus can be used to develop viable suspensions of anchorage-dependent cells.
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http://dx.doi.org/10.1007/s10616-020-00408-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450034PMC
August 2020

Electrochemical Immunoassay for Determination of Glycated Albumin using Nanozymes.

Sci Rep 2020 06 11;10(1):9513. Epub 2020 Jun 11.

Department of Bionano Engineering, Hanyang University, Ansan, 426 - 791, South Korea.

We developed a new nanozyme-based electrochemical immunoassay method for the monitoring of glycated albumin (GA) known to reflect short-term glycaemic levels. For this study, we synthesized urchin-like Pt nanozymes (uPtNZs) and applied them to colorimetric and electrochemical assays for sensitive determination of GA in total human serum albumin (tHSA) using 3,3',5,5'-tetramethylbenzidine (TMB) and thionine as substrates, respectively. The uPtNZs showed peroxidase-mimic activity in the presence of hydrogen peroxide. Boronic acid (BA)-agarose bead was used to capture GA through specific cis-diol interactions. uPtNZs were modified with GA antibody (GA-Ab) to form sandwich complexes with GA/BA-agarose bead. The amount of Ab-uPtNZ/GA/BA-agarose bead complex increased with increasing percentage of GA in 50 mg/mL tHSA. The colorimetric assay exhibited linearity from 0.02 to 10% (10 µg/mL - 5 mg/mL) GA with an LOD of 0.02% (9.2 µg/mL). For electrochemical assay, GA was detected from 0.01 to 20% (5 µg/mL - 10 mg/mL) with an LOD of 0.008% (3.8 µg/mL). The recovery values of measured GA in human plasma samples were from 106 to 107%. These results indicate that electrochemical assay using uPtNZs is a promising method for determining GA.
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http://dx.doi.org/10.1038/s41598-020-66446-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289889PMC
June 2020

Determination of glycated albumin using boronic acid-derived agarose beads on paper-based devices.

Biomicrofluidics 2018 Jan 1;12(1):014111. Epub 2018 Feb 1.

Department of Bionano Engineering, Hanyang University, Ansan 426-791, South Korea.

Self-monitoring of glycated albumin (GA), a useful glycemic marker, is an established method for preventing diabetes complications. Here, the paper-based lateral flow assay devices were developed for the sensitive detection of GA and the total human serum albumin (tHSA) in self-monitoring diabetes patients. Boronic acid-derived agarose beads were packed into a hole on a lateral flow channel. These well-coordinated agarose beads were used to capture GA through specific cis-diol interactions and to enhance the colorimetric signals by concentrating the target molecules. The devices exhibited large dynamic ranges (from 10 g/ml to 10 mg/ml for GA and from 10 mg/ml to 50 mg/ml for tHSA) and low detection limits (7.1 g/ml for GA and 4.7 mg/ml for tHSA), which cover the range of GA concentration in healthy plasma, which is 0.21-1.65 mg/ml (0.6%-3%). In determining the unknown GA concentrations in two commercial human plasma samples, the relative percentage difference between the values found by a standard ELISA kit and those found by our developed devices was 2.62% and 8.80%, which are within an acceptable range. The measurements of GA and tHSA were completed within 20 min for the total sample-to-answer diagnosis, fulfilling the demand for rapid analysis. Furthermore, the recovery values ranged from 99.4% to 110% in device accuracy tests. These results indicate that the developed paper-based device with boronic acid-derived agarose beads is a promising platform for GA and tHSA detection as applied to self-monitoring systems.
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http://dx.doi.org/10.1063/1.5021395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796828PMC
January 2018

Predictors of urgency improvement after Holmium laser enucleation of the prostate in men with benign prostatic hyperplasia.

Investig Clin Urol 2016 11 24;57(6):431-436. Epub 2016 Oct 24.

Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Purpose: To investigate the change in urinary urgency and predictors of urgency improvement after holmium laser enucleation of the prostate (HoLEP) in men with benign prostatic hyperplasia (BPH).

Materials And Methods: We retrospectively analyzed the medical records of patients who were treated with HoLEP for BPH and had preoperative urgency measuring ≥3 on a 5-point urinary sensation scale. Those with prostate cancer diagnosed prior to or after HoLEP, a history of other prostatic and/or urethral surgery, moderate to severe postoperative complications, and neurogenic causes were excluded. Patients who had improved urgency with antimuscarinic medication after HoLEP were excluded. We divided the patients into 2 groups based on urgency symptoms 3 months after HoLEP: improved and unimproved urgency. Improved urgency was defined as a reduction of 2 or more points on the 5-point urinary sensation scale. Preoperative clinical and urodynamic factors as well as perioperative factors were compared between groups.

Results: In total, 139 patients were included in this study. Voiding parameters in all patients improved significantly after HoLEP. Seventy-one patients (51.1%) had improved urgency, while 68 (48.9%) did not show any improvement. A history of acute urinary retention (AUR) and postvoid residual were associated with postoperative urgency improvement in univariate analysis. In multivariate analysis, a history of AUR was an independent factor affecting urgency improvement.

Conclusions: A preoperative history of AUR could influence the change in urgency after HoLEP surgery in patients with BPH.
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http://dx.doi.org/10.4111/icu.2016.57.6.431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109790PMC
November 2016

A scaffold-free surface culture of B16F10 murine melanoma cells based on magnetic levitation.

Cytotechnology 2016 Dec 26;68(6):2323-2334. Epub 2016 Sep 26.

Department of Bioengineering and Technology, Kangwon National University, Chuncheon, 200-701, Korea.

Multicellular spheroids are obtained in a variety of three-dimensional (3D) culture systems without the use of supporting scaffold. We present here a 3D culture method that resulted in a multicellular sheet under scaffold-free conditions. A floating disk-shaped 3D culture was prepared by magnetic levitation of B16F10 cells that has ingested FeO-containing fibroin microspheres. The melanoma disk grew up to 19 mm in diameter and the thickness was ranged between 80 and 100 μm. The 3D culture was filled with closely packed cells that were proliferating exponentially at a specific growth rate of µ = 0.015 h. Approximately half of the cells were Ki-67 positive with no detectable levels of apoptotic or autophagic cells. However, the percentage of propidium iodide-permeable cells was 8.5 ± 1.2 %, which was probably due to physical damage in the cell membrane caused by FeO-containing microspheres under a strong magnetic field. Melanin production increased by a factor of 3.0-3.7 in the 3D culture, due to an increased population of pigmented cells. This study presented a surface 3D culture of B16F10 cells without the use of a scaffold based on magnetic levitation.
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http://dx.doi.org/10.1007/s10616-016-0026-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101302PMC
December 2016

Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells.

Authors:
Jin Sil Lee Won Hur

Int J Nanomedicine 2016 13;11:2069-79. Epub 2016 May 13.

Department of Bioengineering and Technology, Kangwon National University, Chuncheon, South Korea.

Purified fibroin protein can be obtained in large quantities from silk fibers and processed to form microscopic particles as delivery vehicles for therapeutic agents. In this study, we demonstrated that fibroin microspheres were taken up by 3T3 cells, localized in the nonlysosomal compartment, and secreted from the cytoplasm after medium replenishment. DNA-loaded microspheres were taken up by >95% of 3T3 cells. DNA cargo had no influence on the intracellular trafficking of microspheres, while fluorescently labeled cargo DNA was observed in the lysosomal compartment and in the microspheres. These results indicate that fibroin microspheres can travel through 3T3 cells without making any contact with the lysosomal compartments. The amount of DNA loaded in the microspheres taken up by 3T3 cells was estimated up to 831.0 pg/cell. Thus, fibroin microspheres can deliver a large amount of randomly fragmented DNA (<10 kb) into the cytoplasmic compartment of 3T3 cells.
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http://dx.doi.org/10.2147/IJN.S103830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874634PMC
March 2017

The Effect of Valsalva Leak Point Pressure on Outcomes of the Needleless ®System in Female Stress Urinary Incontinence.

Urol J 2015 Sep 4;12(4):2251-5. Epub 2015 Sep 4.

Department of Urology, The Catholic University of Korea, Bucheon St. Mary`s Hospital, 327 Sosa-Ro, Wonmi-gu, Bucheon-city, Gyeonggi-do, 420-717, Korea.

Purpose: This study aimed to investigate the effects of preoperative Valsalva leak point pressure (VLPP) on the outcomes of the single-incision midurethral sling procedure (Needleless® System) in female stress urinary incon­tinence (SUI).

Materials And Methods: We evaluated 112 patients who underwent midurethral sling placement for SUI using the Needleless® System. Patients were divided into two groups according to their preoperative VLPP values: VLPP >90 cmH2O (group 1) and VLPP 60-90 cmH2O (group 2). After the postoperative period, SUI status and satisfac­tion were compared between the two groups. Subjective cure was defined as the absence of any episodes of urinary incontinence associated with conditions that increase intra-abdominal pressure in daily life. Treatment satisfaction was analyzed according to patient responses as 'satisfied', 'neutral', and 'dissatisfied'. Postoperative other lower urinary tract symptoms except SUI were compared between the two groups too.

Results: There were no significant differences in age, body weight, and urodynamic parameters (except VLPP) between the two groups. The mean VLPPs were 105.9 ± 12.3 cmH2O (range, 93.6-118.2 cmH2O) in group 1 and 75.4 ± 10.5 cmH2O (range, 65-85.9 cmH2O) in group 2. The overall subjective cure rates were 65.0% in group 1 and 62.5% in group 2 (P = .744). The overall satisfaction rates were 58.8% in group 1 and 68.8% in group 2 (P = .600). Complication rates did not differ between the two groups.

Conclusion: When stratified as > 90 cmH2O or ≤ 90 cmH2O, preoperative VLPP did not affect Needleless® Sys­tem outcomes in female SUI patients.
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September 2015

The effect of clonidine pretreatment on epidural resiniferatoxin in a neuropathic pain rat model.

Acta Med Okayama 2015 ;69(2):95-103

Department of Anesthesiology and Pain Medicine, Gachon University Gil Hospital, Incheon 405-760, Korea.

Resiniferatoxin (RTX) is an ultrapotent synthetic TRPV1 (transient receptor potential vanilloid subtype 1) agonist with significant initial transient hyperalgesia followed by a prolonged analgesic effect in response to thermal stimulus. Using a rat model of neuropathic pain, we evaluated the effect of pretreatment with clonidine-which has been shown to relieve intradermal capsaicin-induced hyperalgesia-on the initial hyperalgesic response and the thermal analgesic property of RTX. Thirty-six male rats were divided into 6 treatment groups (n=6 each):RTX 500 ng, RTX 1 μg, clonidine 20 μg (Cl), Cl+RTX 500 ng, Cl+RTX 1 μg, or normal saline 20 μL (control). We evaluated the short-term (180 min) and long-term (20 days) analgesic effects of RTX after thermal stimulation and mechanical stimulation. RTX had significant initial transient hyperalgesia followed by a prolonged analgesic effect in response to the thermal stimulus, but the RTX 500 ng and RTX 1 μg groups showed no initial short-term thermal hyperalgesic responses when pretreated with clonidine. The Cl+RTX 1 μg rats' behavior scores indicated that they were more calm and comfortable compared to the RTX 1 μg rats. Even though we cannot precisely confirm that pretreatment with clonidine potentiates or adds to the analgesic effect of RTX, clonidine pretreatment with epidural RTX eliminated the initial RTX-associated hyperalgesic response and systemic toxicity in this neuropathic pain rat model.
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http://dx.doi.org/10.18926/AMO/53338DOI Listing
February 2016

DNA ligase4 as a prognostic marker in nasopharyngeal cancer patients treated with radiotherapy.

Asian Pac J Cancer Prev 2014 ;15(24):10985-9

Department of Internal Medicine, Dong-A University College of Medicine, Busan, Korea E-mail :

Background: The capability for DNA double-strand breaks (DSBs) repair is crucial for inherent radiosensitivity of tumor and normal cells. We have investigated the clinicopathologic significance of DNA repair gene expression in nasopharyngeal (NP) carcinoma.

Materials And Methods: A total of 65 NP cancer patients who received radiotherapy were included. The immunopositivity to Ku 70, DNA-PKcs, MRN, RAD50, XRCC4, and LIG4 were examined in all tumor tissues.

Results: The patients comprised 42 males and 23 females, with a median age of 56 years (range, 18-84). The expression levels of RAD50 (0,+1,+2,+3) were 27.7%, 32.3%, 21.5%, and 18.5%. LIG4 (±) were 43.1% and 56.9% respectively. The 5-year OS rate of patients with LIG4 (±) were 90% and 67.9%, respectively (p=0.035). The 5-year TTP rate of patients with LIG4 (±) were 75.9%, 55.5%, respectively (P=0.039).

Conclusions: Our results suggest the possibility of predicting the radiosensitivity of NP cancer by performing immunohistochemical analysis of LIG4.
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http://dx.doi.org/10.7314/apjcp.2014.15.24.10985DOI Listing
October 2015

Growth, cell cycle progression, and morphology of 3T3 cells following fibroin microsphere ingestion.

J Biomed Mater Res A 2015 Apr 14;103(4):1325-31. Epub 2014 Jul 14.

Department of Bioengineering and Technology, Kangwon National University, Chuncheon, 200-701, Korea.

Cellular uptake of microspheres may cause physiological stress and toxicity. In this report, we investigated the effect of cellular uptake of fibroin microspheres on the growth, cell cycle progression, and morphology of 3T3 cells. The microspheres were prepared by physical cross-linking of fibroin molecules without any chemical modification. Fluorescent microspheres are comprised of fluorescein isothiocyanate-dextran core and fibroin shell. More than 90% of cells were determined to be fluorescence-positive following 24-h incubation with fluorescent microspheres (0.17 mg/mL). Microsphere localization in the cytoplasm was demonstrated using confocal and transmission electron microscopy. Cellular uptake of microspheres did not influence cellular viability, but microsphere concentrations above 0.1 mg/mL resulted in decreased cell proliferation. The proliferation inhibition was attributed to G2 /M phase delay in cell cycle progression and S-phase delay at higher microsphere concentrations (0.33 mg/mL). Although flow cytometry light-scattering data raised the possibility of morphological changes, Coulter counter analysis confirmed no significant size differences between cells incubated with and without microspheres. Accordingly, fibroin microspheres can be a potential vehicle for intracytoplasmic delivery of cargos, without affecting cell viability.
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http://dx.doi.org/10.1002/jbm.a.35269DOI Listing
April 2015

Scaffold-free formation of a millimeter-scale multicellular spheroid with an internal cavity from magnetically levitated 3T3 cells that ingested iron oxide-containing microspheres.

Authors:
Joon Ho Lee Won Hur

Biotechnol Bioeng 2014 May 6;111(5):1038-47. Epub 2013 Dec 6.

Department of Bioengineering and Technology, College of Engineering, Kangwon National University, 291-1 Hyoja-dong, Chuncheon, 200-701, Korea.

This report describes fabrication of a millimeter-scale three-dimensional (3D) multicellular structure with a central cavity based on magnetic levitation of 3T3 cells that had ingested Fe3 O4 -containing microcapsules. Magnetically levitated cells initially formed a disc-shaped cell cluster at the air-medium interface and transformed into a spheroid (up to 2.8 mm in diameter) after 10-day incubation under a magnet. Hematoxylin-and-eosin-stained section revealed that an eosinophilic shell of cells enclosed a pale-staining core of the spheroid. Mitotic or elongated and aligned cells were found at the outer periphery of the shell, while Fe3 O4 deposits were distributed in the inner part of the shell. Surgical dissection indicated that the spheroid had a hollow interior filled with a fluid-state cell suspension. Accordingly, it was demonstrated that magnetically levitated 3T3 cells organized themselves into a tissue-like spheroid, resulting in core cell death. The spheroid can be used as a 3D tissue model and as building blocks that fused to form a more complicated structure.
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http://dx.doi.org/10.1002/bit.25156DOI Listing
May 2014

Repeat vertebroplasty for the subsequent refracture of procedured vertebra.

Korean J Pain 2013 Jan 4;26(1):94-7. Epub 2013 Jan 4.

Department of Anesthesiology and Pain Medicine, Korea University Guro Hospital, Seoul, Korea.

Vertebroplasty (VP) can effectively treat pain and immobility caused by vertebral compression fracture. Because of complications such as extravasation of bone cement (polymethylmethacrylate, PMMA) and adjacent vertebral fractures, some practitioners prefer to inject a small volume of PMMA. In that case, however, insufficient augmentation or a subsequent refracture of the treated vertebrae can occur. A 65-year-old woman visited our clinic complaining of unrelieved severe low back and bilateral flank pain even after she had undergone VP on the 1(st) and 4(th) (L1 and L4) lumbar vertebrae a month earlier. Radiologic findings showed the refracture of L1. We successfully performed the repeat VP by filling the vertebra with a sufficient volume of PMMA, and no complications occurred. The patient's pain and immobility resolved completely three days after the procedure and she remained symptom-free a month later. In conclusion, VP with small volume cement impaction may fail to relieve fracture-induced symptoms, and the refracture of an augmented vertebral body may occur. In this case, repeat VP can effectively resolve both the persistent symptoms and problems of new onset resulting from refracture of the augmented vertebral body due to insufficient volume of bone cement.
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http://dx.doi.org/10.3344/kjp.2013.26.1.94DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546220PMC
January 2013

In vitro cellular uptake of fibroin microspheres and its dependency on the cell cycle stage.

J Microencapsul 2013 31;30(2):124-31. Epub 2012 Jul 31.

Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Korea.

Cellular uptake of a protein microsphere was investigated in vitro. Fibroin microspheres with an average diameter of approximately 1 µm were prepared with Fluorescein isothiocyanate-dextran for the fluorescent core. Tomography, performed using confocal laser scanning microscopy, confirmed microsphere uptake into the cytoplasmic area of 3T3 cells. Flow cytometry showed that cellular uptake was proportional to the co-incubation time and the microsphere concentration. It also revealed that fibroin microspheres were internalised by 3T3 cells at different rates depending on the cell cycle stage. The following cell stages had higher concentrations of internalised microspheres: G(2)/M > S > G(0)/G(1), when using 0.1 mg of microspheres per 10(6) cells. The internalised microspheres per cell also increased in the same order of cell cycle stages when co-incubating cells with 1 mg of microspheres. These results provide information that can be used to develop fibroin microspheres for intracellular delivery of large cargos.
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http://dx.doi.org/10.3109/02652048.2012.704951DOI Listing
July 2013

Amelioration of the progression of an atopic dermatitis-like skin lesion by silk peptide and identification of functional peptides.

Biosci Biotechnol Biochem 2012 ;76(3):473-7

Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

The efficacy of silk peptide in treatment of atopic dermatitis was examined in a picryl chloride-induced atopic dermatitis model in NC/Nga mice. Silk peptide ameliorated the development of atopic dermatitis by lowering the serum IgE concentration. Treatment of cultured spleen cells with silk peptide reduced IgE production by enhancing the production of IFN-γ and reducing the level of IL-4. The functional peptides in the silk peptide were identified as mixture of GAGA sequences containing peptides by mass spectrometry and in vitro assay. Our findings indicate that silk peptide exerts an effect on atopic dermatitis by modulating the Th1/Th2 balance.
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http://dx.doi.org/10.1271/bbb.110748DOI Listing
July 2012

Treatment outcomes between concurrent chemoradiotherapy and combination of surgery, radiotherapy, and/or chemotherapy in stage III and IV maxillary sinus cancer: multi-institutional retrospective analysis.

J Oral Maxillofac Surg 2012 Jul 25;70(7):1717-23. Epub 2011 Sep 25.

Department of Internal Medicine, Institute of Health Science, Gyeongsang National University College of Medicine, Jinju, Korea.

Purpose: The incidence of maxillary sinus cancer (MSC) is extremely rare, representing less than 1% of all cancers. Because of its rarity, the management of locally advanced MSC is a challenging issue. The objective of the present study was to retrospectively compare the efficacy of 2 traditional treatment strategies, concurrent chemoradiotherapy (CCRT) versus combination of surgery and radiotherapy and/or chemotherapy (SRCT) in MSC.

Patients And Methods: From 1989 to 2010, 65 patients with histologically confirmed stage III or IVA/IVB were retrospectively analyzed.

Results: The median age of our subjects was 60 years (range 36 to 81). The present study involved 18 women (27.7%) and 47 men (72.3%). Of the 65 patients, 52 (80.0%) had squamous cell carcinoma. The TNM stage was stage III, as determined by the American Joint Committee on Cancer, 6th edition, in 27 patients (41.5%). Stage IVA or IVB was observed in 38 patients (58.5%). Of the 65 patients, 41 underwent treatment. Of these 41 patients, 26 and 15 patients underwent SRCT and CCRT, respectively. During the 75.6 months (range 6.4 to 249.4) of median follow-up, the median progression-free survival duration was 45.1 months (95% confidence interval 0.0 to 142.7). The 5-year overall survival rate was 64.8%. However, the patients who had undergone surgery had better progression-free survival (hazard ratio 2.363, 95% confidence interval 1.098 to 5.085, P = .028) and overall survival (hazard ratio 4.989, 95% confidence interval 1.646 to 15.118, P = .004). The SRCT group had a better progression-free survival (P = .043) and overall survival (P = .029) duration than did the CCRT group.

Conclusion: SRCT might be superior to CCRT for locally advanced MSC. Additional studies comparing the treatment outcomes of CCRT with SRCT are warranted.
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http://dx.doi.org/10.1016/j.joms.2011.06.221DOI Listing
July 2012

Longevity of organic layers of vertical flow ponds for sulfate reduction in treating mine drainages in South Korea.

Environ Geochem Health 2012 Jan 4;34 Suppl 1:115-21. Epub 2011 Aug 4.

Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources, Daejeon, South Korea.

This study was carried out to evaluate longevity of available organic materials used for sulfate-reducing bacteria (SRB) activity in vertical flow ponds (VFPs) to treat mine drainage in South Korea. Spent mushroom compost samples (SMC) were tested as substrates in VFPs and analyzed for total organic carbon in VFPs, and were collected to analyze total organic carbon (TOC), T-N, T-P, K, metals and residual cellulose to check the longevity assessment. Chemical analysis revealed that the average contents of Fe, Al and Mn in SMC of VFPs were 19,907, 32,137 and 434 mg/kg, respectively. The contents of Fe and Al in SMC of VFPs were much higher than those of the unused SMC (control), but to the contrary, those of Mn showed a reversed tendency. Average TOC content of the controls was 64.19% but in one of the VFP substrates was as low as 15.92%. This might be resulted from SRB consumed the available organic carbon in SMC as VFPs system aged. Contents of T-N in VFPs tended to decrease as VFPs aged. The residual cellulose ranged from 3.88 to 6.72% (g/g). There existed a negative relationship between residual cellulose contents and ages of VFPs. Assuming that SMC in all VFPs had similar compositions when the VFPs were initially established, trend analysis predicted that the amount of carbon source for SRB might be available for 12-15 years further, depending on VFPs.
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http://dx.doi.org/10.1007/s10653-011-9404-4DOI Listing
January 2012

Development of inactivated trivalent vaccine for the teratogenic Aino, Akabane and Chuzan viruses.

Biologicals 2011 May 21;39(3):152-7. Epub 2011 Mar 21.

National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Anyang, Republic of Korea.

Aino, Akabane and Chuzan viruses are arthropod-borne (arbo) viruses transmitted by blood-sucking insects like mosquitoes and Culicoides biting midges. These arbovirus infections are mainly associated with abortion, stillbirth and congenital defects in pregnant cattle, sheep and goats, which induces a considerable economic loss in livestock industry. The viruses seem to be widely distributed in Southeast Asia and Australia. As a control strategy, an inactivated trivalent vaccine against Aino, Akabane and Chuzan virus was developed by using binary ethylenimine or formalin as an inactivating agent. The newly developed trivalent vaccine is evaluated for its safety and immunogenicity in animals such as mice, guinea pigs and cattle. The immune responses were significantly detected within 2-weeks after second vaccination without any side effects. Since the field application of experimental vaccine also revealed increased antibodies in inoculated cattle, we demonstrated that these trivalent vaccines could be used as a vaccine to control the arboviral infections in ruminants.
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http://dx.doi.org/10.1016/j.biologicals.2011.02.004DOI Listing
May 2011

Even-numbered peptides from a papain hydrolysate of silk fibroin.

Authors:
Jaeho Jeong Won Hur

J Chromatogr B Analyt Technol Biomed Life Sci 2010 Mar 29;878(9-10):836-40. Epub 2010 Jan 29.

The Center for Cell Signaling & Drug Discovery Research, Ewha Womans University, Seoul 120-750, Republic of Korea.

A protease with broad substrate specificity usually produces a complex peptide mixture. However, even-numbered peptides were obtained at high proportion upon papain hydrolysis of fibroin composed of highly repetitive Ala- and Gly-rich blocks. MALDI-TOF and ESI mass spectrometric analysis revealed that the even-numbered peptides were in the forms of di-, tetra-, hexa-, and octa-peptides with repeating units in combination of Ala-Gly, Ser-Gly, Tyr-Gly, and Val-Gly. Application of tandem mass spectrometry identified the sequences of the tetra-peptides to be in the order of Ala-Gly-X-Gly (X = Tyr or Val). Therefore, the substrate specificity of papain and the unique repetitive sequence of fibroin generated the hydrolysate composed of even number of amino acids at a high percentage. In this work, fibroin hydrolysate was investigated as an example of an end product of protein hydrolysis, which provides a clue to understand the fate of peptides in a protein hydrolysate.
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http://dx.doi.org/10.1016/j.jchromb.2010.01.034DOI Listing
March 2010

Clinical outcomes of self-expandable metal stent and prognostic factors for stent patency in gastric outlet obstruction caused by gastric cancer.

Dig Dis Sci 2010 Mar 31;55(3):668-74. Epub 2009 Mar 31.

Division of Gastroenterology, Department of Internal Medicine, The Catholic University, Seoul, Korea.

The aim of this study was to assess clinical outcomes of endoscopic stenting for a gastric outlet obstruction caused by gastric cancer and the prognostic factors for stent patency by reviewing medical records. Eighty-one stents were inserted into 75 patients (48 men, average age 66 years). The technical and clinical success rates were 98 and 87%, respectively. The median stent patency was 55 days (95% CI 40-70 days). The median survival was 79 days (95% CI 58-123 days). Stent reobstruction caused by tumor ingrowth or overgrowth occurred in 25 cases (31%). Cox multivariate regression analysis showed that covered stents (odds ratio 0.29, 95% CI 0.11-0.76; P = 0.01) and chemotherapy after stent placement (odds ratio 0.34, 95% CI 0.13-0.91; P = 0.03) were significant prognostic factors for stent patency. This study found that endoscopic stenting is a safe and effective palliation treatment for malignant gastric outlet obstruction and a covered stent and chemotherapy are significant prognostic factors for stent patency.
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http://dx.doi.org/10.1007/s10620-009-0787-3DOI Listing
March 2010

[A case report of treatment with pegylated interferon alpha for lamivudine-resistant chronic hepatitis B virus infection].

Korean J Hepatol 2008 Dec;14(4):513-8

Department of Internal Medicine, College of Medicine, The Catholic University of Korea, WHO Collaborating Center of Viral Hepatitis, Seoul, Korea.

The wide use of lamivudine in chronic hepatitis B has produced a monotonic increase in patients with lamivudine resistance. Therefore, treating lamivudine resistance in chronic hepatitis B is a major concern in clinical practice for the treatment of hepatitis B virus (HBV). There is conflicting evidence on the outcome of pegylated interferon alpha (PEG-IFN alpha) therapy against lamivudine-resistant HBV, which is due to mutations in the YMDD motif. We experienced a patient with chronic hepatitis B who was successfully treated with PEG-IFN alpha-2a after the development of virologic and biochemical breakthrough during lamivudine therapy. Virologic breakthrough was associated with the emergence of YMDD mutants 48 months after starting lamivudine therapy. Treatment with PEG-IFN alpha-2a for 12 months resulted in an undetectable serum level of HBV DNA and the resolution of hepatitis, and the virologic response was maintained over 16 months after cessation of PEG-IFN alpha-2a.
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http://dx.doi.org/10.3350/kjhep.2008.14.4.513DOI Listing
December 2008

Impact of intensity-modulated radiation therapy as a boost treatment on the lung-dose distributions for non-small-cell lung cancer.

Int J Radiat Oncol Biol Phys 2005 Nov 31;63(3):683-9. Epub 2005 May 31.

Department of Radiation Oncology, Dong-A University School of Medicine, Busan, South Korea.

Purpose: To investigate the feasibility of intensity-modulated radiotherapy (IMRT) as a method of boost radiotherapy after the initial irradiation by the conventional anterior/posterior opposed beams for centrally located non-small-cell lung cancer through the evaluation of dose distributions according to the various boost methods.

Methods And Materials: Seven patients with T3 or T4 lung cancer and mediastinal node enlargement who previously received radiotherapy were studied. All patients underwent virtual simulation retrospectively with the previous treatment planning computed tomograms. Initial radiotherapy plans were designed to deliver 40 Gy to the primary tumor and involved nodal regions with the conventional anterior/posterior opposed beams. Two radiation dose levels, 24 and 30 Gy, were used for the boost radiotherapy plans, and four different boost methods (a three-dimensional conformal radiotherapy [3DCRT], five-, seven-, and nine-beam IMRT) were applied to each dose level. The goals of the boost plans were to deliver the prescribed radiation dose to 95% of the planning target volume (PTV) and minimize the volumes of the normal lungs and spinal cord irradiated above their tolerance doses. Dose distributions in the PTVs and lungs, according to the four types of boost plans, were compared in the boost and sum plans, respectively.

Results: The percentage of lung volumes irradiated >20 Gy (V20) was reduced significantly in the IMRT boost plans compared with the 3DCRT boost plans at the 24- and 30-Gy dose levels (p = 0.007 and 0.0315 respectively). Mean lung doses according to the boost methods were not different in the 24- and 30-Gy boost plans. The conformity indexes (CI) of the IMRT boost plans were lower than those of the 3DCRT plans in the 24- and 30-Gy plans (p = 0.001 in both). For the sum plans, there was no difference of the dose distributions in the PTVs and lungs according to the boost methods.

Conclusions: In the boost plans the V20s and CIs were reduced significantly by the IMRT plans, but in the sum plans the effects of IMRT on the dose distributions in the tumor and lungs, like CI and V20, were offset. Therefore, to keep the beneficial effect of IMRT in radiotherapy for lung cancer, it would be better to use IMRT as a whole treatment plan rather than as a boost treatment.
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http://dx.doi.org/10.1016/j.ijrobp.2005.03.012DOI Listing
November 2005

Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica, Pasteurella multocida and Actinobacillus pleuropneumoniae.

J Vet Sci 2002 Mar;3(1):47-57

Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon, 441-744, Korea.

Swine respiratory diseases induce severe economic losses in the swine industry worldwide. Several methods have been developed and applied to control these diseases. However, there are still problems of disease control in the swine industry. Recently, egg yolk antibodies have been found to offer several advantages for disease control in animals and humans. In a previous study (24), antibodies to several causative pathogens of swine respiratory diseases were developed. However, several problems remained, especially in terms of reduced laying rates. Therefore, experimental vaccines were reformulated with various bacterial antigens of the swine respiratory diseases. After immunizing hens with the antigens, antibody profiles and other effects including laying rates were investigated and compared to those of the previous study. Profiles of antibody titers were very similar with those of the previous study. However, side effects, such as depression, weakness, reduction of laying rates and mortality, were dramatically lowered and laying rates were increased in hens injected with certain experimental vaccines. In particular, laying rates of hens injected with vaccines against atrophic rhinitis were increased up to 84% by injecting a vaccine composed of only the DNTs of B. bronchiseptica and P. multocida D:4. Efficacies of the vaccines against swine pneumonic pasteurellosis and pleuropneumonia were very similar with those of the previous study. These results suggest that new vaccines could be effective in the production of egg yolk antibodies against the causative agents of swine respiratory diseases.
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March 2002

[A case of inflammatory pseudotumor of the spleen].

Korean J Gastroenterol 2003 Aug;42(2):168-71

Department of Internal Medicine, The Catholic University of Korea, College of Medicine, Seoul, Korea.

Inflammatory pseudotumor is a benign disease, which is histologically composed of the inflammatory cells such as mature lymphocytes, plasma cells, and histiocytes. It usually occurs in the respiratory system, liver, central nervous system, and gastrointestinal tracts. However, inflammatory pseudotumor rarely occurs in the spleen. Pathologic diagnosis is essential for the definitive diagnosis because of the difficulty in distinguishing pseudotumor from lymphoproliferative disorders of the spleen. We report a case of inflammatory pseudotumor of the spleen. A 35-year-old woman complained of the intermittent epigastric pain for several months. Physical examination and laboratory findings were normal. Ultrasonography and abdominal computerized tomography showed a low attenuation splenic mass suggesting lymphoma. However, the pathologic findings of the resected spleen were consistent with those of the inflammatory pseudotumor. The spleen weighed 230 g containing a 6 x 5 x 5 cm-sized, well-circumscribed gray mass. The microscopic findings indicated the inflammatory cell infiltrations.
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August 2003