William C Skarnes

William C Skarnes

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William C Skarnes

William C Skarnes

Publications by authors named "William C Skarnes"

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Improving homology-directed repair efficiency in human stem cells.

Methods 2019 07 16;164-165:18-28. Epub 2019 Jun 16.

The Jackson Laboratory for Genomic Medicine, Farmington, CT 06032, USA.

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http://dx.doi.org/10.1016/j.ymeth.2019.06.016DOI Listing
July 2019

Response to "Unexpected mutations after CRISPR-Cas9 editing in vivo".

Nat Methods 2018 04 30;15(4):235-236. Epub 2018 Mar 30.

IMPC Cas9 Working Group and the IMPC Steering Committee and executive director of the IMPC (www.mousephenotype.org).

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http://dx.doi.org/10.1038/nmeth.4559DOI Listing
April 2018

ZFP57 and the Targeted Maintenance of Postfertilization Genomic Imprints.

Cold Spring Harb Symp Quant Biol 2015 ;80:177-87

Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom.

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http://dx.doi.org/10.1101/sqb.2015.80.027466DOI Listing
January 2018

Corrigendum: High-throughput discovery of novel developmental phenotypes.

Nature 2017 11 8;551(7680):398. Epub 2017 Nov 8.

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http://dx.doi.org/10.1038/nature24643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5849394PMC
November 2017

One-step generation of conditional and reversible gene knockouts.

Nat Methods 2017 03 30;14(3):287-289. Epub 2017 Jan 30.

Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, UK.

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http://dx.doi.org/10.1038/nmeth.4156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5777571PMC
March 2017

CRISPR-Cas9 enables conditional mutagenesis of challenging loci.

Sci Rep 2016 09 1;6:32326. Epub 2016 Sep 1.

Institute of Developmental Genetics, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg/Munich, Germany.

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http://dx.doi.org/10.1038/srep32326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007477PMC
September 2016

High-throughput discovery of novel developmental phenotypes.

Nature 2016 09 14;537(7621):508-514. Epub 2016 Sep 14.

The Jackson Laboratory, Bar Harbor, Maine 04609, USA.

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http://dx.doi.org/10.1038/nature19356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295821PMC
September 2016

WGE: a CRISPR database for genome engineering.

Bioinformatics 2015 Sep 14;31(18):3078-80. Epub 2015 May 14.

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

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http://dx.doi.org/10.1093/bioinformatics/btv308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4565030PMC
September 2015

Off-target mutations are rare in Cas9-modified mice.

Nat Methods 2015 Jun;12(6):479

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.

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http://dx.doi.org/10.1038/nmeth.3408DOI Listing
June 2015

Is mouse embryonic stem cell technology obsolete?

Genome Biol 2015 May 27;16:109. Epub 2015 May 27.

Stem Cell Engineering, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.

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http://dx.doi.org/10.1186/s13059-015-0673-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443626PMC
May 2015

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.

Nat Methods 2014 Apr 2;11(4):399-402. Epub 2014 Mar 2.

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.

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http://www.nature.com/articles/nmeth.2857
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http://dx.doi.org/10.1038/nmeth.2857DOI Listing
April 2014

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.

Nucleic Acids Res 2014 Jan 4;42(Database issue):D802-9. Epub 2013 Nov 4.

European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK, Medical Research Council Harwell (Mammalian Genetics Unit and Mary Lyon Centre), Harwell, Oxfordshire OX11 0RD, UK and Mouse Informatics Group, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

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http://dx.doi.org/10.1093/nar/gkt977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964955PMC
January 2014

The mammalian gene function resource: the International Knockout Mouse Consortium.

Mamm Genome 2012 Oct 12;23(9-10):580-6. Epub 2012 Sep 12.

The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK.

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http://dx.doi.org/10.1007/s00335-012-9422-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463800PMC
October 2012

Accessing data from the International Mouse Phenotyping Consortium: state of the art and future plans.

Mamm Genome 2012 Oct 19;23(9-10):641-52. Epub 2012 Sep 19.

Mammalian Genetics Unit, Medical Research Council Harwell, Harwell, Oxfordshire OX11 0RD, UK.

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http://dx.doi.org/10.1007/s00335-012-9428-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106044PMC
October 2012

Building and sharing resources. Interview by Krystie Nybo.

Biotechniques 2012 May;52(5):299

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http://dx.doi.org/10.2144/000113846DOI Listing
May 2012

BioMart as an integration solution for the International Knockout Mouse Consortium.

Database (Oxford) 2011 18;2011:bar028. Epub 2011 Sep 18.

The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH.

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http://dx.doi.org/10.1093/database/bar028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263594PMC
January 2012

Bi-allelic gene targeting in mouse embryonic stem cells.

Methods 2011 Apr 1;53(4):331-8. Epub 2011 Feb 1.

Wellcome Trust Sanger Institute, Morgan Building, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom.

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http://dx.doi.org/10.1016/j.ymeth.2010.12.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3572411PMC
April 2011

Cop1 constitutively regulates c-Jun protein stability and functions as a tumor suppressor in mice.

J Clin Invest 2011 Apr 14;121(4):1329-43. Epub 2011 Mar 14.

Laboratory for Molecular Cancer Biology, Department of Molecular and Developmental Genetics, VIB-K.U.Leuven, Leuven, Belgium.

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http://dx.doi.org/10.1172/JCI45784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070608PMC
April 2011

Dual RMCE for efficient re-engineering of mouse mutant alleles.

Nat Methods 2010 Nov 17;7(11):893-5. Epub 2010 Oct 17.

Developmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.

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http://dx.doi.org/10.1038/nmeth.1521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3576631PMC
November 2010

Floxin, a resource for genetically engineering mouse ESCs.

Nat Methods 2010 Jan 6;7(1):50-2. Epub 2009 Dec 6.

Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.

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http://dx.doi.org/10.1038/nmeth.1406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895430PMC
January 2010

Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

Nat Methods 2009 Jul 14;6(7):493-5. Epub 2009 Jun 14.

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK.

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http://dx.doi.org/10.1038/nmeth.1342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3555078PMC
July 2009

Gene trapping in mouse embryonic stem cells.

Methods Mol Biol 2008 ;461:133-48

Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK.

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http://dx.doi.org/10.1007/978-1-60327-483-8_9DOI Listing
January 2009

Extensive genomic copy number variation in embryonic stem cells.

Proc Natl Acad Sci U S A 2008 Nov 6;105(45):17453-6. Epub 2008 Nov 6.

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom.

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http://dx.doi.org/10.1073/pnas.0805638105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582305PMC
November 2008

ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a.

Proc Natl Acad Sci U S A 2008 May 30;105(18):6656-61. Epub 2008 Apr 30.

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Richard Simches Research Center, 185 Cambridge Street, Boston, MA 02114, USA.

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http://dx.doi.org/10.1073/pnas.0801802105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373334PMC
May 2008

Tectonic, a novel regulator of the Hedgehog pathway required for both activation and inhibition.

Genes Dev 2006 Jan 15;20(1):22-7. Epub 2005 Dec 15.

Developmental and Stem Cell Biology Program, and Diabetes Center, University of California, San Francisco, 94143-0525, USA.

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http://dx.doi.org/10.1101/gad.1363606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1356097PMC
January 2006

Two ways to trap a gene in mice.

Proc Natl Acad Sci U S A 2005 Sep 6;102(37):13001-2. Epub 2005 Sep 6.

Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom.

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http://dx.doi.org/10.1073/pnas.0506279102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1201614PMC
September 2005

JAM-A expression during embryonic development.

Dev Dyn 2005 Aug;233(4):1517-24

Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA.

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http://dx.doi.org/10.1002/dvdy.20481DOI Listing
August 2005

Endoplasmic reticulum stress induction of the Grp78/BiP promoter: activating mechanisms mediated by YY1 and its interactive chromatin modifiers.

Mol Cell Biol 2005 Jun;25(11):4529-40

Department of Biochemistry and Molecular Biology, USC/Norris Comprehensive Cancer Center, Keck School of Medicine, 1441 Eastlake Ave., Room 5308, MC-9176, Los Angeles, CA 90089-9176, USA.

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http://mcb.asm.org/cgi/doi/10.1128/MCB.25.11.4529-4540.2005
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http://dx.doi.org/10.1128/MCB.25.11.4529-4540.2005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1140640PMC
June 2005

Polybromo protein BAF180 functions in mammalian cardiac chamber maturation.

Genes Dev 2004 Dec;18(24):3106-16

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA.

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http://dx.doi.org/10.1101/gad.1238104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC535920PMC
December 2004

Embryonic fibroblasts with a gene trap mutation in Ext1 produce short heparan sulfate chains.

J Biol Chem 2004 Jul 25;279(31):32134-41. Epub 2004 May 25.

Department of Medical Biochemistry and Microbiology, Uppsala University, Biomedical Center, P.O. Box 582, SE-751 23 Uppsala, Sweden.

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http://dx.doi.org/10.1074/jbc.M312624200DOI Listing
July 2004

The Wnt co-receptors Lrp5 and Lrp6 are essential for gastrulation in mice.

Development 2004 Jun 13;131(12):2803-15. Epub 2004 May 13.

Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3200, USA.

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http://dx.doi.org/10.1242/dev.01137DOI Listing
June 2004

ATP-citrate lyase deficiency in the mouse.

J Biol Chem 2004 Mar 8;279(10):9557-64. Epub 2003 Dec 8.

Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94141, USA.

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http://dx.doi.org/10.1074/jbc.M310512200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2888281PMC
March 2004

Expression of laminin chains by central neurons: analysis with gene and protein trapping techniques.

Genesis 2003 Jun;36(2):114-27

Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA.

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http://dx.doi.org/10.1002/gene.10206DOI Listing
June 2003

Defining the importance of phosphatidylserine synthase 2 in mice.

J Biol Chem 2002 Dec 1;277(49):47701-8. Epub 2002 Oct 1.

Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141-9100, USA.

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http://www.jbc.org/lookup/doi/10.1074/jbc.M207734200
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http://dx.doi.org/10.1074/jbc.M207734200DOI Listing
December 2002

Maternal effect for DNA mismatch repair in the mouse.

Genetics 2002 Jan;160(1):271-7

Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1461957PMC
January 2002