Publications by authors named "Wenyu Fang"

8 Publications

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Incidence and risk factors associated with the development of epilepsy in patients with intracranial alveolar echinococcosis.

Epilepsy Res 2021 Aug 27;174:106643. Epub 2021 Apr 27.

Department of Neurology, West China Hospital, Sichuan University, No. 37 Guoxue Road, Chengdu, Sichuan, 610041, People's Republic of China. Electronic address:

Parasitic infection remains a critical health problem in Ganzi Tibetan Autonomous Prefecture of China. The association of epilepsy and intracranial alveolar echinococcosis (IAE) is still largely unclear. This study primarily aimed to assess both the incidence and possible risk factors of epilepsy in patients with IAE. According to the occurrence of seizures, patients were separated into two different groups consisting of patients with epilepsy and those without epilepsy. Univariate and multivariate logistic regression analysis was used to identify the potential risk factors associated with the development of epilepsy in patients with IAE. A total of 97 patients (42 women, 55 men; age 19-76 years) were enrolled. Epilepsy was observed in almost 20 % of patients with IAE. The use of anti-seizure medications was not standardized, as 83.3 % of female patients of childbearing age used sodium valproate. It was observed that cortical lesions (hazard ratio (HR) = 29.740, P = 0.006) were significantly associated with development of epilepsy. In addition, epilepsy had no significant effect on the overall survival rate of patients with IAE.
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http://dx.doi.org/10.1016/j.eplepsyres.2021.106643DOI Listing
August 2021

Clinical Features, Radiological Characteristics, and Outcomes of Patients With Intracranial Alveolar Echinococcosis: A Case Series From Tibetan Areas of Sichuan Province, China.

Front Neurol 2020 15;11:537565. Epub 2021 Jan 15.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

Intracranial alveolar echinococcosis (IAE), a zoonotic disease, is a critical health problem in the Tibetan region. We aimed to describe the clinical and radiological characteristics and outcomes among patients with IAE. We screened patients diagnosed with IAE between March 2015 and May 2019 at the Ganzi Tibetan Autonomous Prefecture People's Hospital. Detailed demographics, clinical characteristics, neuroimaging features, and outcomes were recorded. A total of 21 patients with an average age of 44.1 ± 12.7 years were included. Thirteen (61.9%) patients were male. The most common chief neurological complaint was headache ( = 17, 81.0%), followed by dizziness, seizure, visual disturbances, hemiparesis, disturbed consciousness, and dysphasia. All the patients had coexisting liver localizations. The typical neuroimaging features of IAE on cerebral magnetic resonance imaging scans showed obvious low-signal shadow with multiple small vesicles inside the lesions on T2-weighted images and FLAIR images. The pathological HE staining demonstrates vesicular lesions with several internal sacs. For hepatic alveolar echinococcosis (AE), the hepatic portal was invaded in six (28.6%) patients, and the portal vein ( = 5, 23.8%) was the mostly commonly involved vessel. As for treatment, 11 patients (52.4%) had poor compliance with albendazole. The duration of patients taken albendazole ranged from 2 months to 3 years. Cerebral AE surgery was performed in 11 patients, five of them underwent partial resection of AE lesions, and six patients received total resection. One patient with primary IAE underwent radical surgery. Ten patients (47.6%) died during the follow-up for a mean of 21.7 ± 11.9 (3-46) months. In total, 28.9% of the patients died within 5 years, and 71.6% died within 10 years. The median interval between the date of diagnosis as AE and death was 84 (19-144) months. Despite substantial advances in diagnostic and therapeutic methods, the treatment of IAE remains difficult and results in unsatisfactory outcomes. The major critical issue is surgical treatment of IAE although the disease is disseminated. Besides, lifelong albendazole would be indicated, but most patients had poor medication compliance. It is important to educate patients about the necessity of medical treatment.
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http://dx.doi.org/10.3389/fneur.2020.537565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843382PMC
January 2021

Identification and Comparison of microRNAs in the Gonad of the Yellowfin Seabream ().

Int J Mol Sci 2020 Aug 8;21(16). Epub 2020 Aug 8.

School of Marine Sciences, Sun Yat-sen University, Zhuhai 519082, China.

Yellowfin seabream () is a commercially important fish in Asian coastal waters. Although natural sex reversal has been described in yellowfin seabream, the mechanisms underlying sexual differentiation and gonadal development in this species remain unclear. MicroRNAs (miRNAs) have been shown to play crucial roles in gametogenesis and gonadal development. Here, two libraries of small RNAs, constructed from the testes and ovaries of yellowfin seabream, were sequenced. Across both gonads, we identified 324 conserved miRNAs and 92 novel miRNAs: 67 ovary-biased miRNAs, including the miR-200 families, the miR-29 families, miR-21, and miR-725; and 88 testis-biased miRNAs, including the let-7 families, the miR-10 families, miR-7, miR-9, and miR-202-3p. GO (Gene Ontology) annotations and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses of putative target genes indicated that many target genes were significantly enriched in the steroid biosynthesis pathway and in the reproductive process. Our integrated miRNA-mRNA analysis demonstrated a putative negatively correlated expression pattern in yellowfin seabream gonads. This study profiled the expression patterns of sex-biased miRNAs in yellowfin seabream gonads, and provided important molecular resources that will help to clarify the miRNA-mediated post-transcriptional regulation of sexual differentiation and gonadal development in this species.
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http://dx.doi.org/10.3390/ijms21165690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461063PMC
August 2020

Gonadal Transcriptome Analysis of Sex-Related Genes in the Protandrous Yellowfin Seabream ().

Front Genet 2020 16;11:709. Epub 2020 Jul 16.

School of Marine Sciences, Sun Yat-sen University, Zhuhai, China.

Yellowfin seabream (), a protandrous hermaphroditic fish, is a good model for studying the mechanism of sex reversal. However, limited knowledge is known about the genetic information related to reproduction and sex differentiation in this species. Here, we performed transcriptome sequencing analysis of the testis, ovotestis, and ovary to identify sex-related genes in yellowfin seabream. The results assembled 71,765 unigenes in which 16,126 and 17,560 unigenes were differentially expressed in the ovotestis and ovary compared to the testis, respectively. The most differentially expressed gene (DEG)-enriched Kyoto Encyclopedia of Genes and Genomes and GO pathways were closely associated with the synthesis of sex steroid hormones. Functional analyses identified 55 important sex-related DEGs, including 32 testis-biased DEGs (, , and , etc.), 20 ovary-biased DEGs (, , and , etc.), and 3 ovotestis-biased DEGs (, , and ). Furthermore, the testis-specific expression of and the brain-pituitary-ovary axis expression of were characterized, suggesting that they might play important roles in sex differentiation in yellowfin seabream. Our present work provided an important molecular basis for elucidating the mechanisms underlying sexual transition and reproductional regulation in yellowfin seabream.
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http://dx.doi.org/10.3389/fgene.2020.00709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378800PMC
July 2020

Response of gut microbiota and immune function to hypoosmotic stress in the yellowfin seabream (Acanthopagrus latus).

Sci Total Environ 2020 Nov 15;745:140976. Epub 2020 Jul 15.

School of Marine Sciences, Sun Yat-sen University, Zhuhai 519082, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519080, China. Electronic address:

Osmotic stress is associated with heightened immune functions and altered microbiota in the fish intestine. In this study, we explored the effects of hypoosmotic stress on the intestine of euryhaline yellowfin seabream (Acanthopagrus latus) after acute exposure to brackish water, low-saline water, and freshwater environments. The results showed that hypoosmotic stress reshaped the composition of the microbial community and altered the gene expression in the intestine. Probiotics Lactobacillus and Pseudomonas showed higher relative abundance in a brackish water environment, whereas pathogenic bacteria, including Vibrio and Aeromonas, were more abundant in the freshwater environment. At the transcriptional level, osmoregulation-related genes were identified as up/down regulated differentially expressed genes (DEGs) as well as a series of immune-related DEGs associated with pathogen recognition, antimicrobial ability, pro-inflammatory cytokines, cell apoptosis, and antioxidant defense. Physiological analysis showed that Na K-ATPase activity was significantly inhibited by hypoosmotic stress in freshwater. Meanwhile, the intestinal antioxidant defense system of yellowfin seabream was challenged. Correlation network analysis demonstrated the close interactions among intestinal microbes, differentially expressed genes, and physiological parameters. This study provides the critical insights into the function of the intestine fish encountering hypoosmotic stress.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140976DOI Listing
November 2020

Hypoosmotic stress induced tissue-specific immune responses of yellowfin seabream (Acanthopagrus latus) revealed by transcriptomic analysis.

Fish Shellfish Immunol 2020 Apr 16;99:473-482. Epub 2020 Feb 16.

School of Marine Sciences, Sun Yat-sen University, Zhuhai, 519082, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519080, China. Electronic address:

Salinity is a limiting factor for many marine organisms, including fishes. The shift in the ambient salinity can cause osmotic stress and arouse immune responses in fish. In this study, yellowfin seabream (Acanthopagrus latus), a euryhaline marine teleost, was used to investigate immune responses of different tissues (gill, liver, and muscle) under hypoosmotic stress. Comparative transcriptomic and physiological analyses of three tissues were conducted after fish exposed to the fresh water (FW, salinity = 0 ppt), low-saline water (LW, salinity = 3 ppt), and brackish water (BW, salinity = 6 ppt) for 8 days. The results showed that hypoosmotic stress dramatically altered the gene expression of three tissues in yellowfin seabream; The investigation of differentially expressed genes (DEGs) related to osmoregulation and immune response indicated that T cell-mediate immunity pathways were essential to tackle such stress. In terms of tissues, gill was found to be the most sensitive tissue under hypoosmotic stress by enhancing of NaK-ATPase activity and preventing the loss of Na and K; Liver, on the other hand, was under the most sever oxidative stress indicated by the fluctuation of SOD, CAT activities and the MDA content; In contrast, muscle had the least osmoregulation and immune related response. We also identified several potential candidate genes, which may serve as gene indicators to identify the stressor. Overall, this study provides preliminary mechanistic insights into hypoosmotic stress adaption of aquatic organism.
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http://dx.doi.org/10.1016/j.fsi.2020.02.028DOI Listing
April 2020

Construction of High-Density Genetic Map and Mapping of Sex-Related Loci in the Yellow Catfish (Pelteobagrus fulvidraco).

Mar Biotechnol (NY) 2020 Feb 2;22(1):31-40. Epub 2020 Jan 2.

Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China.

The yellow catfish (Pelteobagrus fulvidraco) is a very important aquaculture species distributed in freshwater area of China. All-male yellow catfish is very popular in aquaculture because of their significant sex dimorphism phenomena. The males grow much faster than females in full-sibling family. However, the sex dimorphism mechanism is still unclear in yellow catfish. In order to better understand the genetic basis of yellow catfish sexual dimorphism, it is vital to map the sex-related traits and localize the candidate genes across yellow catfish whole genome. Here, we constructed a high-density linkage map of yellow catfish using genotyping-by-sequencing (GBS) strategy. A total of 5705 single-nucleotide polymorphism (SNP) markers were mapped to 26 different linkage groups (LGs) using 184 F1 offspring. The total genetic map length was 3071.59 cM, with an average interlocus distance of 0.54 cM. Eleven significant sex-related QTLs in yellow catfish were identified. Six sex-related genes were identified from the region of reference genome near these QTLs including amh, gnrhr, vasa, lnnr1, foxl2, and bmp15. The high-density genetic linkage map provides valuable resources for yellow catfish molecular assistant breeding and elucidating sex differentiation process. Moreover, the comparative genomic study was analyzed among yellow catfish, channel catfish, and zebrafish. It revealed highly conserved chromosomal distribution between yellow catfish and channel catfish.
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http://dx.doi.org/10.1007/s10126-019-09928-4DOI Listing
February 2020

A Retrospective Analysis of the Clinical Features of Inpatients With Epilepsy in the Ganzi Tibetan Autonomous Prefecture.

Front Neurol 2018 30;9:891. Epub 2018 Oct 30.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

There is limited detailed clinical information for patients with epilepsy in Tibet. This study sought to provide data about the clinical features of epilepsy in the Ganzi Tibetan Autonomous Prefecture to improve strategies for epilepsy prevention and management in this region. We reviewed the clinical record of patients with epilepsy in the Neurology Department, Ganzi Tibetan Autonomous Prefecture People's Hospital and compared the clinical features and compared it with control, from West China Hospital in Chengdu. This retrospective study included 165 patients with epilepsy admitted between January 2015 and February 2018. Majority of patients (97%) in this study had active epilepsy; 28.5% had generalized onset seizures and 68.5% had focal onset seizures. Fifty-four patients had received anti-epileptic drug (AED) treatment prior to hospitalization, however, 38 (70.4%) patients took the medication irregularly. The leading etiology of this cohort was head trauma (20.6%), followed by stroke (10.9%), neurocysticercosis (7.9%), brain hydatidosis (6.7%) and tuberculous infection (5.5%). Compared with in-patients in Chengdu, epilepsy in Ganzi was more frequently caused by infection (OR = 4.216, 95% CI, 2.124-8.367), including neurocysticercosis (OR = 29.301, 95% CI, 1.727-497.167) and brain hydatidosis (OR = 24.637, 95% CI, 1.439-421.670). These data suggest that the control of cerebral infections, especially parasite infection, is essential for the prevention of epilepsy in the Ganzi Tibetan Autonomous Prefecture. Education of local primary doctors and patients about the literacy of epilepsy will enable better management of epilepsy in this population.
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http://dx.doi.org/10.3389/fneur.2018.00891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218953PMC
October 2018