Publications by authors named "Wenyan Li"

219 Publications

Generation of mature and functional hair cells by co-expression of Gfi1, Pou4f3, and Atoh1 in the postnatal mouse cochlea.

Cell Rep 2021 Apr;35(3):109016

ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200031, China; Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China; NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai 200031, China; The Institutes of Brain Science and the Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China. Electronic address:

The mammalian cochlea cannot regenerate functional hair cells (HCs) spontaneously. Atoh1 overexpression as well as other strategies are unable to generate functional HCs. Here, we simultaneously upregulated the expression of Gfi1, Pou4f3, and Atoh1 in postnatal cochlear supporting cells (SCs) in vivo, which efficiently converted SCs into HCs. The newly regenerated HCs expressed HC markers Myo7a, Calbindin, Parvalbumin, and Ctbp2 and were innervated by neurites. Importantly, many new HCs expressed the mature and terminal marker Prestin or vesicular glutamate transporter 3 (vGlut3), depending on the subtypes of the source SCs. Finally, our patch-clamp analysis showed that the new HCs in the medial region acquired a large K current, fired spikes transiently, and exhibited signature refinement of ribbon synapse functions, in close resemblance to native wild-type inner HCs. We demonstrated that co-upregulating Gfi1, Pou4f3, and Atoh1 enhances the efficiency of HC generation and promotes the functional maturation of new HCs.
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http://dx.doi.org/10.1016/j.celrep.2021.109016DOI Listing
April 2021

The relationship between childhood emotional abuse and depressive symptoms among Chinese college students: The multiple mediating effects of emotional and behavioral problems.

J Affect Disord 2021 Mar 31;288:129-135. Epub 2021 Mar 31.

Department of Psychiatry, University of Toronto, Toronto, ON, Canada.

Background: This study aims to explore the mediational effects of emotional and behavioral problems on the association between childhood emotional abuse and depressive symptoms among college students.

Methods: Data were drawn from 60 universities from 10 provinces in China (n=30,374). Information about childhood maltreatment, depressive symptoms, emotional and behavioral problems were gathered through the Childhood Trauma Questionnaire-Short Form (CTQ-SF), the Center for Epidemiologic Studies Depression Scale (CES-D) and the Strengths and Difficulties Questionnaire (SDQ), respectively. Univariable and multivariable logistic regression models and mediating models were used.

Results: After controlling for demographic factors, childhood emotional abuse was the strongest risk factor for depressive symptoms (adjusted odds ratio (aOR)=2.54, 95%CI=2.27-2.85). The relationship between childhood emotional abuse and depressive symptoms was partially mediated by emotional and behavioral problems with 68.7% total indirect effect. Among the 5 identified subtypes of emotional and behavioral problems, the mediating effects of emotional problems (57.3%) and hyperactivity (28.6%) were higher than peer problems (7.8%) and prosocial behavior (3.6%). Conduct problems did not show a significant mediating effect (p>0.05).

Limitations: The cross-sectional design is limited to make inferences about causality.

Conclusions: Childhood emotional abuse was strongly associated with depressive symptoms in college students. Of the five identified subtypes of emotional and behavioral problems, four subtypes mediated the relationship between childhood emotional abuse and depressive symptoms, including emotional problems, hyperactivity, peer problems and prosocial behavior.
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http://dx.doi.org/10.1016/j.jad.2021.03.074DOI Listing
March 2021

Carbon dots for specific "off-on" sensing of Co and EDTA for in vivo bioimaging.

Mater Sci Eng C Mater Biol Appl 2021 Apr 10;123:112022. Epub 2021 Mar 10.

Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, PR China; Department of Chemistry, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China. Electronic address:

Fluorescent carbon dots (CDs) were hydrothermally synthesized from a mixture of frozen tofu, ethylenediamine and phosphoric acid in an efficient 64% yield. The resulting CDs exhibit good water solubility, low cytotoxicity, high stability, and excellent biocompatibility. The CDs selectively and sensitively detect Co through fluorescent quenching with a detection limit of 58 nM. Fluorescence can be restored through the introduction of EDTA, and this phenomenon can be used to quantify EDTA in solution with a detection limit of 98 nM. As both analytes are detected by the same CD platform, this is an "off-on" fluorescence sensor for Co and EDTA. The technique's robustness for real-world samples was illustrated by quantifying cobalt in tap water and EDTA in contact lens solution. The CDs were also evaluated for in vivo imaging as they show low cytotoxicity and excellent cellular uptake. In a zebrafish model, the CDs are rapidly adsorbed from the intestine into the liver, and are essentially cleared from the body in 24 h with no appreciable bioaccumulation. Their simple and efficient synthesis, combined with excellent physical and chemical performance, renders these CDs attractive candidates for theranostic applications in targeted "smart" drug delivery and bioimaging.
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http://dx.doi.org/10.1016/j.msec.2021.112022DOI Listing
April 2021

HSP70-3 Interacts with Phospholipase Dδ and Participates in Heat Stress Defense.

Plant Physiol 2021 Apr;185(3):1148-1165

College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. China.

Heat shock proteins (HSPs) function as molecular chaperones and are key components responsible for protein folding, assembly, translocation, and degradation under stress conditions. However, little is known about how HSPs stabilize proteins and membranes in response to different hormonal or environmental cues in plants. Here, we combined molecular, biochemical, and genetic approaches to elucidate the involvement of cytosolic HSP70-3 in plant stress responses and the interplay between HSP70-3 and plasma membrane (PM)-localized phospholipase Dδ (PLDδ) in Arabidopsis (Arabidopsis thaliana). Analysis using pull-down, coimmunoprecipitation, and bimolecular fluorescence complementation revealed that HSP70-3 specifically interacted with PLDδ. HSP70-3 bound to microtubules, such that it stabilized cortical microtubules upon heat stress. We also showed that heat shock induced recruitment of HSP70-3 to the PM, where HSP70-3 inhibited PLDδ activity to mediate microtubule reorganization, phospholipid metabolism, and plant thermotolerance, and this process depended on the HSP70-3-PLDδ interaction. Our results suggest a model whereby the interplay between HSP70-3 and PLDδ facilitates the re-establishment of cellular homeostasis during plant responses to external stresses and reveal a regulatory mechanism in regulating membrane lipid metabolism.
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http://dx.doi.org/10.1093/plphys/kiaa083DOI Listing
April 2021

Role of organic anion transporter 3 in the renal excretion of biapenem and potential drug-drug interactions.

Eur J Pharm Sci 2021 Mar 19;162:105814. Epub 2021 Mar 19.

Department of Pharmacy, Shanghai Pudong New Area Gongli Hospital, The Second Military Medical University, 219 Miaopu Road, Shanghai 200135, PR China. Electronic address:

Biapenem is a carbapenem antibiotic. It is excreted predominantly through the kidney as unchanged forms. However, the molecular mechanism of renal excretion of biapenem and potential drug-drug interactions (DDIs) were still unknown. In the present study, the role of organic anion transporters (OAT) 1/3 and organic cation transporters (OCT) 2 in the renal excretion of biapenem, and the potential DDIs between biapenem and six clinical commonly prescribed antibiotics and antiviral drugs that acted as substrates or inhibitors of OAT3 were evaluated in vitro. Further, the effect of probenecid on the pharmacokinetics of biapenem was explored in the rats. We observed that biapenem could not inhibit the transport activities of OAT1 or OCT2, while mildly inhibited OAT3 (IC >500 μM). Among the tested antibiotics and antiviral drugs, the relatively high DDI index values (maximal unbound plasma concentration over IC, I/IC) were found for piperacillin, linezolid and benzylpenicillin, which were 2.84, 1.7 and 0.62, respectively. Although probenecid had the highest DDI index (27.1) in vitro, no significant impact of it on the pharmacokinetics of biapenem was observed in the rats. Our results indicated that biapenem was primarily eliminated by the glomerular filtration, while OAT3-mediated renal tubular secretion was a minor route. Biapenem is not a clinically relevant substrate or inhibitor because of its low affinity to OAT3. According to current results, it would be safe to use biapenem with other antibiotics and antiviral drugs that acted as substrates or inhibitors of OAT3.
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http://dx.doi.org/10.1016/j.ejps.2021.105814DOI Listing
March 2021

Peripheral immune profile of children with Talaromyces marneffei infections: a retrospective analysis of 21 cases.

BMC Infect Dis 2021 Mar 20;21(1):287. Epub 2021 Mar 20.

Department of Respiratory Infection, Guangzhou Medical University, No.9, Jinsui Road, Zhujiang New City, Tianhe District, Guangzhou, 510120, Guangdong, China.

Background: Talaromyces marneffei (T. marneffei) is an opportunistic pathogen that infects immunodeficient children. The aim of the study is to determine the clinical features and peripheral immune state of Talaromyces marneffei (T. marneffei) infections in children for early detection and diagnosis.

Methods: We retrospectively reviewed 21 pediatric patients who were diagnosed with T. marneffei infections and were followed up in the Guangzhou Women and Children's Medical Center from January 2010 to January 2020. For each patient, we collected and analyzed clinical characteristics, peripheral immunological results, genetic tests, complications and prognosis.

Results: Common clinical features of the patients included fever (20/21, 95.24%), cough (17/21, 80.95%) and hepatomegaly (17/21, 80.95%). Severe complications included septic shock (12/21, 57.14%), hemophagocytic lymphohistiocytosis (HLH) (11/21, 52.38%), acute respiratory distress syndrome (ARDS) (10/21, 47.62%), multiple organ dysfunction syndrome (MODS) (9/21, 42.86%), and disseminated intravascular coagulation (DIC) (7/21, 33.33%). Eleven children (11/21, 52.38%) eventually died of T. marneffei infections. All patients were HIV negative. Seven cases revealed reduced antibody levels, especially IgG. Higher levels of IgE were detected in 9 cases with an obvious increase in two patients. Ten patients showed decreased complement C3 levels, some of whom had low C4 levels. Three patients displayed decreased absolute T lymphocyte counts, including the CD 4+ and CD 8+ subsets. A reduction in NK cells was present in most patients. No patient had positive nitro blue tetrazolium (NBT) test results. Nine patients were screened for common genetic mutations. Of the cases, one case had no disease-specific gene mutation. Four children had confirmed hyperimmunoglobulin M syndrome (HIGM) with CD40LG variation, one case had severe combined immunodeficiency (SCID), and one case had hyper-IgE syndrome (HIES). One patient was identified as having a heterozygous mutation in STAT3 gene; however, he showed no typical clinical manifestations of HIES at his age. One patient had a mutated COPA gene with uncertain pathogenic potential. Another patient was diagnosed with HIES that depended on her clinical features and the National Institutes of Health (NIH) scoring system.

Conclusions: T. marneffei infections in HIV-negative children induced severe systemic complications and poor prognosis. Children with T. marneffei infections commonly exhibited abnormal immunoglobulin levels in peripheral blood, particularly decreased IgG or increased IgE levels, further suggesting possible underlying PIDs in these patients.
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http://dx.doi.org/10.1186/s12879-021-05978-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7980795PMC
March 2021

The mechanism of lncRNA-CRNDE in regulating tumour-associated macrophage M2 polarization and promoting tumour angiogenesis.

J Cell Mol Med 2021 Mar 20. Epub 2021 Mar 20.

Department of Center Laboratory, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China.

M2 macrophages can promote liver cancer metastasis by promoting tumour angiogenesis; however, the mechanism underlying macrophage polarization has not been completely revealed. In this study, we mainly explored the mechanism underlying long non-coding RNA-CRNDE (lncRNA-CRNDE) in regulating M2 macrophage polarization and promoting liver cancer angiogenesis. The expression of CRNDE was up-regulated or down-regulated in THP-1 cells (CRNDE -THP-1 cells and pcDNA3.1-CRNDE-THP-1). THP-1 cells were co-cultured with liver cancer cell line H22, and M2 polarization was induced in THP-1 by IL-4/13 to simulate tumour-induced macrophage polarization. As a result, after CRNDE overexpression, THP-1 cell viability was up-regulated, the expression of M2 membrane marker CD163 was up-regulated, and the proportion of F4/80 + CD163+ cells was also up-regulated. ELISA assay showed that the expression of M2 markers (including TGF-β1 and IL-10) and chemokines (including CCl22 and CCL22) was up-regulated, and the expression of key signals (including STAT6, JAK-1, p-AKT1, and Arg-1) was also up-regulated, which were significantly different compared with the control group (Con). In addition, the intervention effect of CRNDE on THP-1 was consistent between co-culture with H22 cells and IL-4/13 induction assay. The induced M2 THP-1 cells were co-cultured with HUVEC. As a result, THP-1 cells with CRNDE overexpression can promote the migration and angiogenesis of HUVEC cells in vitro and simultaneously up-regulate the expression of Notch1, Dll4 and VEGFR2, indicating that THP-1 M2 polarization induced by CRNDE could further promote angiogenesis. The H22 cell tumour-bearing mouse model was constructed, followed by injection of CRNDE anti-oligosense nucleotides and overexpression plasmids to interfere CRNDE expression in tumour-bearing tissues. Consequently, down-regulation of CRNDE could down-regulate tumour volume, simultaneously down-regulate the expression of CD163 and CD31 in tissues, decrease the expression of key proteins (including JAK-1, STAT-6, p-STAT6 and p-AKT1), and down-regulate the expression of key angiogenesis-related proteins (including VEGF, Notch1, Dll4 and VEGFR2). In this study, we found that CENDE could indirectly regulate tumour angiogenesis by promoting M2 polarization of macrophages, which is also one of the mechanisms of microenvironmental immune regulation in liver cancer.
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http://dx.doi.org/10.1111/jcmm.16477DOI Listing
March 2021

MiR-706 alleviates white matter injury via downregulating PKCα/MST1/NF-κB pathway after subarachnoid hemorrhage in mice.

Exp Neurol 2021 Mar 11;341:113688. Epub 2021 Mar 11.

Department of Neurosurgery and State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Third Military Medical University (Army Military Medical University), Chongqing 400038, China; Chongqing Key Laboratory of Precision Neuromedicine and Neuroregenaration, Southwest Hospital, Third Military Medical University (Army Military Medical University), Chongqing 400038, China.

Increasing numbers of patients with spontaneous subarachnoid hemorrhage(SAH) who recover from surgery and intensive care management still live with cognitive impairment after discharge, indicating the importance of white matter injury at the acute stage of SAH. In the present study, standard endovascular perforation was employed to establish an SAH mouse model, and a microRNA (miRNA) chip was used to analyze the changes in gene expression in white matter tissue after SAH. The data indicate that 17 miRNAs were downregulated, including miR-706, miR-669a-5p, miR-669p-5p, miR-7116-5p and miR-195a-3p, while 13 miRNAs were upregulated, including miR-6907-5p, miR-5135, miR-6982-5p, miR-668-5p, miR-8119. Strikingly, miR-706 was significantly downregulated with the highest fold change. Further experiments confirmed that miR-706 could alleviate white matter injury and improve neurological behavior, at least partially by inhibiting the PKCα/MST1/NF-κB pathway and the release of inflammatory cytokines. These results might provide a deeper understanding of the pathophysiological processes in white matter after SAH, as well as potential therapeutic strategies for the translational research.
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http://dx.doi.org/10.1016/j.expneurol.2021.113688DOI Listing
March 2021

Association between problematic internet use and behavioral/emotional problems among Chinese adolescents: the mediating role of sleep disorders.

PeerJ 2021 22;9:e10839. Epub 2021 Feb 22.

Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-Sen University, Guangzhou, China.

Background: Studies that focus on the relationships of problematic Internet use (PIU), sleep disorders, and behavioral/emotional problems were limited. This study aimed to explore (1) the relationship between PIU and behavioral/emotional problems among Chinese adolescents and (2) whether sleep disorders mediate the relationship between PIU and behavioral/emotional problems.

Methods: A total of 1,976 adolescents were recruited by cluster sampling from ten secondary schools in Guangzhou between January and April 2019, and 1,956 of them provided valid information (response rate: 98.9% ). Among them, 50.8% were males and the mean age was 13.6±1.5 years, ranging from 11 to 18 years. Data on behavioral/emotional problems, sleep disorders, and PIU were collected using a self-reported questionnaire. Linear regression models and mediation analyses were performed.

Results: Of the participants, 14.5% (284/1,956) reported moderate to severe PIU, and their average score for total difficulties was significantly higher than the score for average users (14.9±5.5 Vs 9.8±4.7). After adjusting for controlled variables, PIU was further proven to be positively related to elevated levels of behavioral/emotional problems (unstandardized  = 0.16,  < 0.05). In addition, sleep disorders partially mediated the forgoing associations.

Conclusions: Adolescents with problematic Internet habits were at higher risk of developing behavioral and emotional problems than their normal-use peers, and sleep disorders partially mediated the effect. Close attention and effective guidance for adolescents with PIU and behavioral/emotional problems were recommended for parents and schools.
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http://dx.doi.org/10.7717/peerj.10839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906038PMC
February 2021

Single-Cell Sequencing Applications in the Inner Ear.

Front Cell Dev Biol 2021 12;9:637779. Epub 2021 Feb 12.

ENT Institute and Department of Otorhinolaryngology, Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China.

Genomics studies face specific challenges in the inner ear due to the multiple types and limited amounts of inner ear cells that are arranged in a very delicate structure. However, advances in single-cell sequencing (SCS) technology have made it possible to analyze gene expression variations across different cell types as well as within specific cell groups that were previously considered to be homogeneous. In this review, we summarize recent advances in inner ear research brought about by the use of SCS that have delineated tissue heterogeneity, identified unknown cell subtypes, discovered novel cell markers, and revealed dynamic signaling pathways during development. SCS opens up new avenues for inner ear research, and the potential of the technology is only beginning to be explored.
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http://dx.doi.org/10.3389/fcell.2021.637779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907461PMC
February 2021

Single-Cell Sequencing Applications in the Inner Ear.

Front Cell Dev Biol 2021 12;9:637779. Epub 2021 Feb 12.

ENT Institute and Department of Otorhinolaryngology, Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China.

Genomics studies face specific challenges in the inner ear due to the multiple types and limited amounts of inner ear cells that are arranged in a very delicate structure. However, advances in single-cell sequencing (SCS) technology have made it possible to analyze gene expression variations across different cell types as well as within specific cell groups that were previously considered to be homogeneous. In this review, we summarize recent advances in inner ear research brought about by the use of SCS that have delineated tissue heterogeneity, identified unknown cell subtypes, discovered novel cell markers, and revealed dynamic signaling pathways during development. SCS opens up new avenues for inner ear research, and the potential of the technology is only beginning to be explored.
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http://dx.doi.org/10.3389/fcell.2021.637779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907461PMC
February 2021

Identification of coding region SNPs from specific and sensitive mRNA biomarkers for the deconvolution of the semen donor in a body fluid mixture.

Forensic Sci Int Genet 2021 May 16;52:102483. Epub 2021 Feb 16.

School of Forensic Medicine, Shanxi Medical University, Jinzhong 030619, Shanxi, China. Electronic address:

mRNA markers provide a very promising method for the identification of human body fluids or tissues in the context of forensic investigations. Previous studies have shown that different body fluids can be distinguished from each other according to their specific mRNA biomarkers. In this study, we evaluated eight semen-specific mRNA markers (KLK3, NKX3-1, CKB, KLK2, PRAC1, SEMG1, TGM4, and SORD) that encompass 12 coding single nucleotide polymorphisms (cSNPs) to identify the semen contributor in a mixed stain. Five highly specific and sensitive mRNA markers for blood, menstrual blood, saliva, vaginal secretions, and skin were also incorporated into the PCR system as body fluid-positive controls. Reverse transcription polymerase chain reaction (RT-PCR), multiplex PCR and SNaPshot mini-sequencing assays were established for the identification of semen-specific mRNA. The amplicon size ranged from 133 to 337 bp. The semen-specific system was examined against blood, menstrual blood, saliva, vaginal secretions, and skin swabs. The eight mRNA biomarkers were semen-specific and could be successfully typed in laboratory-generated mixtures composed of different body fluids supplemented with 1 ng of semen cDNA. This system possessed a high sensitivity that ranged from 1:10-1:100 for detecting trace amounts of semen in semen-containing body fluid mixtures. Additionally, our results demonstrated that the cSNPs polymorphisms included in the mRNA markers were concordant with genomic DNA (gDNA). Despite the presence of other body fluids, the system exhibited high sensitivity and specificity to the semen in the mixture. In future studies, we will add other cSNPs from the semen-specific genes using massively parallel sequencing to further improve our system.
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http://dx.doi.org/10.1016/j.fsigen.2021.102483DOI Listing
May 2021

Pyroptosis executive protein GSDMD as a biomarker for diagnosis and identification of Alzheimer's disease.

Brain Behav 2021 Apr 15;11(4):e02063. Epub 2021 Feb 15.

Department of Neurology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China.

Objective: This study was mainly conducted to explore the expression changes of GSDMD and conventional markers (including T-Tau, Tau181p, and Aβ ) in the cerebrospinal fluid among patients with Alzheimer's disease (AD) and vascular dementia (VD), followed by determination of role of GSDMD in diagnosing and identifying AD and VD.

Methods: In this study, 60 patients with VD, 60 patients with AD, and 50 healthy controls were enrolled. Lumbar puncture was performed to collect cerebrospinal fluid samples. Patients with VD and patients with AD were evaluated using the Mini-Mental State Examination (MMSE) scale, Montreal Cognitive Assessment (MoCA) scale, Clinical Dementia Rating (CDR) scale, Activity of Daily Living (ADL) scale, and Neuropsychiatric Inventory (NPI) questionnaire, aiming to determine the behavioral ability of patients. ELISA kit was purchased to determine the levels of GSDMD, T-Tau, Tau181p, and Aβ in cerebrospinal fluid, and the expression of inflammatory factors, IL-1β and IL-6, was also detected.

Results: (1) The levels of GSDMD, T-Tau, and Tau181p in the cerebrospinal fluid were higher in patients with AD than those of patients with VD and healthy controls, while the levels of Aβ in the cerebrospinal fluid were lower in patients with AD than that in healthy controls and patients with VD. (2) GSDMD had good diagnostic accuracy in AD. Additionally, GSDMD, T-Tau, Tau181p, and Aβ had good discrimination accuracy in distinguishing AD and VD. (3) The expression levels of inflammatory factors (IL-1β and IL-6) in cerebrospinal fluid were higher in patients with AD than those of healthy controls and patients with VD, which were positively correlated with GSDMD expression.

Conclusion: The expression of GSDMD was increased in patients with AD, which could be used as a biomarker for AD diagnosis and identification from VD.
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http://dx.doi.org/10.1002/brb3.2063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035446PMC
April 2021

Associations Among Screen Time, Sleep Duration and Depressive Symptoms Among Chinese Adolescents.

J Affect Disord 2021 Apr 2;284:69-74. Epub 2021 Feb 2.

Department of Medical statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou, People's Republic of China. Electronic address:

Objectives: Relatively few studies have explored the inter-relationship between screen time (ST), sleep duration and depressive symptoms. The study herein sought to determine (1) the relationships between ST, sleep duration and depressive symptoms among Chinese adolescents; (2) whether sleep duration mediates the relationships between ST and depressive symptoms.

Methods: 1 grade students (n=1,976) from ten high schools in Guangzhou, China were invited through cluster sampling between January and April 2019. Self-reported ST with electronic devices and Internet, sleep duration, and The Center for Epidemiology Scale for Depression (CES-D) score were collected. Generalized mixed linear models and mediation analyses were conducted.

Results: There were 1,956 self-reported questionnaires received (response rate: 98.99%). Approximately 25% (471/1,929 for Internet use, 399/1,928 for electronic device) of the total sample reported ST >2 hours/day. Approximately 8.9% (169/1,894) reported a CES-D score >28. Longer ST with electronic devices (estimate=0.52, 95%CI: 0.24~0.80), Internet usage (estimate=0.82, 95%CI: 0.53~1.11) were positively associated with depressive symptoms, while less sleep (estimate=-1.85, 95%CI: -2.27~-1.43) was negatively associated with depressive symptoms. There is significant indirect effect of electronic device usage on depressive symptoms through sleep duration (indirect effect=0.08, 95%CI: 0.01~0.15).

Limitations: This study only included school students from Guangzhou. Causal relationship cannot be inferred by this cross-sectional design.

Conclusions: ST and sleep duration were significantly associated with depressive symptoms severity. The indirect effect of sleep duration suggests a possible mechanism of the association between ST and depressive symptoms. Future interventions to manage depressive symptoms should target sleep time and decrease ST among adolescents.
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http://dx.doi.org/10.1016/j.jad.2021.01.082DOI Listing
April 2021

Dendritic cells transduced with TIPE-2 recombinant adenovirus induces T cells suppression.

J Inflamm (Lond) 2021 Feb 10;18(1). Epub 2021 Feb 10.

Department of Neurology and Chongqing Key Laboratory of Cerebrovascular Disease, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, China.

Introduction: TIPE-2 has been identified as a negative regulator of both innate and adaptive immunity and is involved in several inflammatory diseases. However, the role of immune suppression of dendritic cells (DCs) transduced with TIPE-2 has not been well studied.

Methods: In this study, DCs were transduced with TIPE-2 recombinant adenovirus, and then were cocultured with allogeneic CD4+ or CD8 + T cells. The proliferation, cytokine production and activation marker levels of CD4+ or CD8 + T cell were detected.

Results: The data demonstrated that T cell proliferation, cytokine production and activation marker levels were attenuated after treated with TIPE-2 transduced DCs.

Conclusions: These results suggested that TIPE-2 transduced DCs are capable of inducing allogeneic CD4+ or CD8 + T cell immune suppression, which provide a promising way for the therapeutical strategies of transplantation or autoimmune diseases.
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http://dx.doi.org/10.1186/s12950-021-00274-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877089PMC
February 2021

Dendritic cells transduced with TIPE-2 recombinant adenovirus induces T cells suppression.

J Inflamm (Lond) 2021 Feb 10;18(1). Epub 2021 Feb 10.

Department of Neurology and Chongqing Key Laboratory of Cerebrovascular Disease, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, China.

Introduction: TIPE-2 has been identified as a negative regulator of both innate and adaptive immunity and is involved in several inflammatory diseases. However, the role of immune suppression of dendritic cells (DCs) transduced with TIPE-2 has not been well studied.

Methods: In this study, DCs were transduced with TIPE-2 recombinant adenovirus, and then were cocultured with allogeneic CD4+ or CD8 + T cells. The proliferation, cytokine production and activation marker levels of CD4+ or CD8 + T cell were detected.

Results: The data demonstrated that T cell proliferation, cytokine production and activation marker levels were attenuated after treated with TIPE-2 transduced DCs.

Conclusions: These results suggested that TIPE-2 transduced DCs are capable of inducing allogeneic CD4+ or CD8 + T cell immune suppression, which provide a promising way for the therapeutical strategies of transplantation or autoimmune diseases.
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http://dx.doi.org/10.1186/s12950-021-00274-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877089PMC
February 2021

Antiperistaltic effect and safety of l-menthol oral solution on gastric mucosa for upper gastrointestinal endoscopy in Chinese patients: Phase III, multicenter, randomized, double-blind, placebo-controlled study.

Dig Endosc 2021 Feb 1. Epub 2021 Feb 1.

Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Objective: The topical antispasmodic agent l-menthol is commonly used for gastric peristalsis suppression during diagnostic upper gastrointestinal (GI) endoscopy. We evaluated the efficacy and safety of a single dose l-menthol solution in suppressing gastric peristalsis during upper GI endoscopy in Chinese patients.

Methods: In this phase III, multicenter, randomized, double-blind, placebo-controlled study (ClinicalTrials.gov: NCT03263910), 220 patients scheduled to undergo upper GI endoscopy at five Chinese referral centers received a single dose of either 160 mg of l-menthol (n = 109) or placebo (n = 111). Both treatments were sprayed endoscopically on the gastric mucosa. An independent committee evaluated the degree of gastric peristalsis (peristaltic score: grade 1-5).

Results: At baseline, the proportion of patients with grade 1 peristalsis (no peristalsis) did not differ between the groups. The proportion of patients with grade 1 peristalsis post-treatment was significantly higher in the l-menthol group (40.37%, 44/109) versus the placebo group (16.22%, 18/111; P < 0.001); the difference between the groups was 24.15% (95% confidence interval: 12.67%-35.63%; P < 0.001). In the l-menthol group, 61.47% of patients had grade 1 peristalsis after endoscopy versus 24.55% in the placebo group (P < 0.001). The ease of intragastric examination correlated significantly with the grade of peristalsis. The incidence of adverse events was comparable between the groups (P = 0.340).

Conclusions: During upper GI endoscopy, a single dose of l-menthol solution (160 mg) sprayed on the gastric mucosa significantly attenuated gastric peristalsis versus placebo, thereby improving the visual stability without any safety concerns.
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http://dx.doi.org/10.1111/den.13941DOI Listing
February 2021

Identification of -benzothiazolyl-2-benzenesulfonamides as novel ABCA1 expression upregulators.

RSC Med Chem 2020 Mar 11;11(3):411-418. Epub 2020 Mar 11.

Hebei Key Laboratory of Organic Functional Molecules , College of Chemistry and Materials Science , Hebei Normal University , Shijiazhuang , 050024 , China . Email:

ATP binding cassette transporter A1 (ABCA1) is a critical transporter that mediates cellular cholesterol efflux from macrophages to apolipoprotein A-I (ApoA-I). Therefore, increasing the expression level of ABCA1 is anti-atherogenic and ABCA1 expression upregulators have become novel choices for atherosclerosis treatment. In this study, a series of -benzothiazolyl-2-benzenesulfonamides, based on the structure of WY06 discovered in our laboratory, were designed and synthesized as novel ABCA1 expression upregulators. Based on an ABCA1 upregulatory cell model, ABCA1 upregulation of target compounds was evaluated. Compounds , , and have good upregulated ABCA1 expression activities, with EC values of 0.97, 0.37, and 0.41 μM, respectively. A preliminary structure-activity relationship is summarized. Replacing the methoxy group on the benzothiazole moiety of WY06 with a fluorine or chlorine atom and exchanging the ester group with a cyano group resulted in more potent ABCA1 upregulating activity. Moreover, compound increased ABCA1 mRNA and protein expression and significantly promoted cholesterol efflux in RAW264.7 cells. In conclusion, -benzothiazolyl-2-benzenesulfonamides were identified as novel ABCA1 expression upregulators.
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http://dx.doi.org/10.1039/c9md00556kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593777PMC
March 2020

Role of the periotic mesenchyme in the development of sensory cells in early mouse cochlea.

J Otol 2020 Dec 2;15(4):138-143. Epub 2020 Jul 2.

Department of Otolaryngology-Head and Neck Surgery, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, 241001, China.

Objective: To investigate the role of the periotic mesenchyme (POM) in the development of sensory cells of developing auditory epithelium.

Methods: Developing auditory epithelium with or without periotic mesenchyme was isolated from mice at embryonic days 11.5 (E11.5), E12.5 and E13.5, respectively, and cultured to an equivalent of E18.5's epithelium Then, the explants were co-stained with antibodies targeting myosin VIIA, Sox2 and BrdU.

Results: More hair cells in E11.5 + 7 DIV, E12.5 + 6 DIV and E13.5 + 5 DIV auditory epithelia were found upon culture with POM (225.90 ± 62.44, 476.94 ± 100.81, and 1386.60 ± 202.38, respectively) compared with the non-POM group (68.17 ± 23.74, 205.00 ± 44.23, and 1266.80 ± 38.84, respectively). Moreover, regardless of developmental stage, the mesenchymal tissue increased the amount of cochlear sensory cells as well as the ratio of differentiated hair cells to total sensory cells.

Conclusions: The periotic mesenchyme promotes the development of cochlear sensory cells, and its effect depends on the developmental stage of the auditory epithelium.
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http://dx.doi.org/10.1016/j.joto.2020.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691837PMC
December 2020

Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation.

Aging (Albany NY) 2020 11 16;13(1):910-932. Epub 2020 Nov 16.

College of Veterinary Medicine and College of Animal Science and Technology, Hebei Veterinary Biotechnology Innovation Center, Hebei Agricultural University, Baoding 071001, Hebei, China.

Tanshinone IIA (Tan IIA) possesses potent anti-atherogenic function, however, the underlying pharmacological mechanism remains incompletely understood. Previous studies suggest that oxidized LDL (oxLDL)-induced NLRP3 (NOD-like receptor (NLR) family, pyrin domain-containing protein 3) inflammasome activation in macrophages plays a vital role in atherogenesis. Whether the anti-atherogenic effect of Tan IIA relies on the inhibition of the NLRP3 inflammasome has not been investigated before. In this study, we found that Tan IIA treatment of high-fat diet fed ApoE-/- mice significantly attenuated NLRP3 inflammasome activation in vivo. Consistently, Tan IIA also potently inhibited oxLDL-induced NLRP3 inflammasome activation in mouse macrophages. Mechanically, Tan IIA inhibited NF-κB activation to downregulate pro-interleukin (IL) -1β and NLRP3 expression, and decreased oxLDL-induced expression of lectin-like oxidized LDL receptor-1 (LOX-1) and cluster of differentiation 36 (CD36), thereby attenuating oxLDL cellular uptake and subsequent induction of mitochondrial and lysosomal damage - events that promote the NLRP3 inflammasome assembly. Through regulating both the inflammasome 'priming' and 'activation' steps, Tan IIA potently inhibited oxLDL-induced NLRP3 inflammasome activation, thereby ameliorating atherogenesis.
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http://dx.doi.org/10.18632/aging.202202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835056PMC
November 2020

Changes in children's respiratory morbidity and residential exposure factors over 25 years in Chongqing, China.

J Thorac Dis 2020 Oct;12(10):6356-6364

Nicholas School of the Environment and Duke Global Health Institute, Duke University, Durham, NC, USA.

Background: Respiratory morbidity and mortality during childhood remains a major challenge for global health. Due to the rapid economic development in Chongqing, we expect substantial temporal changes in respiratory health status and environmental risk factors in children. By leveraging a historical dataset, this study aims to assess the changes in prevalence of respiratory symptoms and diseases, residential exposure factors, and their associations in school-age children over a period of 25 years.

Methods: This study involved two cross-sectional surveys conducted in Chongqing with a 25-year interval (2017 1993). Purpose sampling was used to conduct questionnaire surveys on school-age children in both surveys. Information collected include children's respiratory health outcomes, family residential exposures, demographic information, and parental respiratory disease history. The changes of residential exposures as well as demographics were determined by chi-square test. Odds ratios were calculated to compare the prevalence of children's respiratory symptoms and diseases between the two periods. Associations between children's respiratory outcomes and exposure indicators were assessed using multivariate logistic regressions.

Results: The majority of residential exposure indicators improved in 2017, including sleep in shared room, cooking with coal, poor kitchen ventilation, cooking frequency, and parental smoking. Compared to the 1993 study, the adjusted risk for children's wheezing was lower (OR: 0.38, 95% CI: 0.29, 0.49), but the risk for bronchitis was higher (OR: 1.89, 95% CI: 1.54, 2.31) in the 2017 study. Poor kitchen ventilation and parental smoking were linked to an increased risk of children's wheezing (OR: 1.39, 95% CI: 1.02, 1.90) and bronchitis (OR: 1.51, 95% CI: 1.02, 2.21), respectively, while heating in winter was linked to an increased risk of phlegm (OR: 1.40, 95% CI: 1.03, 1.90) and wheezing (OR: 1.47, 95% CI: 1.07, 2.01) in the 1993 study. However, these residential exposure factors were no longer associated with the children's respiratory diseases in the 2017 study.

Conclusions: Our study found improvement of residential exposures in Chongqing, a decline of prevalence of children's wheezing but an increase of that of bronchitis from 1993 to 2017. Poor kitchen ventilation, heating in winter, and parental smoking were significant risk factors in the 1993 survey but, with significantly reduced prevalence in 2017, were not significantly associated with children's respiratory morbidity in the latter survey.
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http://dx.doi.org/10.21037/jtd-19-crh-aq-005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656426PMC
October 2020

Effects of indoor environment and lifestyle on respiratory health of children in Chongqing, China.

J Thorac Dis 2020 Oct;12(10):6327-6341

Nicholas School of the Environment and Duke Global Health Institute, Duke University, Durham, NC, USA.

Background: The prevalence of certain respiratory diseases of children in China appears to be on the rise in recent decades. This study aims to explore residential environmental factors that may affect respiratory diseases and lung function of children and to assess the effects of lifestyle (diet and exercise) on lung function.

Methods: The study was conducted in Chongqing, southwest of China in June, 2017. Information on respiratory diseases was obtained from 2,126 primary school children through a family questionnaire by purposive sampling. In addition, a random sample of 771 children participating in the family-questionnaire was selected for physical measurements and lung function test as well as lifestyle questionnaire survey. Chi-square test and multivariate logistic regressions were used to analyze the relationship between indoor environment and children's respiratory diseases. The effects of indoor environment and lifestyle on lung function indices were analyzed by t-test, variance analysis, and univariate and multivariate linear regression methods.

Results: Among residential environmental factors, indoor ventilation and air circulation were significant associated with children's respiratory health outcomes. The use of air conditioning for more than 8 h/day in summer was a risk factor for asthma with an adjusted odds ratio (AOR) of 1.99, bronchitis (AOR =1.62), and allergic rhinitis (AOR =1.51). Ventilation for less than 12 h per day during summer increased the risk for allergic rhinitis (AOR =1.40). Children living in homes with an opened kitchen had the risk of developing allergic rhinitis 1.51 times higher than children living in homes with a closed kitchen. Indoor dampness and mold were significantly associated with increased risks for childhood asthma (AOR =2.16), bronchitis (AOR =1.55) and allergic rhinitis (AOR =1.55). The frequent use of hygienic incense and mosquito coils also increased the risk for asthma (AOR =2.58) and bronchitis (AOR =1.42) in children. The multiple linear regression results showed that frequent use of air fresheners reduced children's peak expiratory flow (PEF) and small airway function (FEF) after potential influencing factors were adjusted for. Analyses of lifestyle variables showed that increased lung function (FVC, FEV, FEV) was associated with increasing consumption of vegetable and fruit as well as increasing time of physical exercise.

Conclusions: This study identified the following residential risk factors for children's respiratory diseases in Chongqing: poor indoor ventilation, home dampness and mold presence, and frequent use of hygienic incense and mosquito coils. Frequent use of air fresheners is associated with reduced lung function in children. High frequency consumption of vegetables, fruits and dairy products as well as daily exercise for more than 1 hour have positive effects on children's lung development.
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http://dx.doi.org/10.21037/jtd.2020.03.102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656398PMC
October 2020

Prevalence of respiratory diseases in relation to smoking rate in adults living in four Chinese cities: a comparison between 2017-2018 and 1993-1996.

J Thorac Dis 2020 Oct;12(10):6315-6326

Beijing Innovation Center for Engineering Science and Advanced Technology, State Key Joint Laboratory for Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, And Center for Environment and Health, Peking University, Beijing, China.

Background: The sustained high prevalence of smoking in China has contributed substantially to the burden of chronic diseases, including respiratory diseases. This study compared the prevalence of smoking and respiratory diseases in Chinese adults between two time periods spanning over 25 years.

Methods: Cross-sectional surveys were performed in four Chinese cities of Chongqing, Lanzhou, Wuhan, and Guangzhou in 1993-1996 (Period 1) and in 2017-2018 (Period 2). Participants completed questionnaires asking smoking status, the presence of asthma and chronic bronchitis, education attainment and household characteristics. Logistic regression models were used to estimate the odds ratios of disease prevalence with regard to active smoking status for men and passive smoking status for women.

Results: Prevalence of asthma, prevalence of chronic bronchitis, and smoking rate, all decreased from Period 1 to Period 2. We observed strong evidence that active smoking increased prevalence for both asthma and chronic bronchitis in men during Period 1, with spatial heterogeneity and modifying effect by college-level education. Home exposure to passive smoking was associated with increased odds of having chronic bronchitis among female participants in Chongqing during Period 2, although the association was not statistically significant.

Conclusions: The prevalence for asthma and chronic bronchitis were lower in 2017-2018 compared to 25 years ago in the same four Chinese cities. Decreased smoking rate may have contribution to the improvement of these respiratory diseases. Male smokers, especially those without college-level education, showed higher prevalence of chronic bronchitis compared to nonsmokers during Period 1.
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http://dx.doi.org/10.21037/jtd-19-crh-aq-002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656329PMC
October 2020

The Mechanism of Aureusidin in Suppressing Inflammatory Response in Acute Liver Injury by Regulating MD2.

Front Pharmacol 2020 28;11:570776. Epub 2020 Oct 28.

Department of Center Laboratory, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China.

Objective: In this study, we mainly explored the mechanism and target of the anti-inflammatory effects of Aureusidin (Aur) in acute liver injury.

Methods: Lipopolysaccharide (LPS) was used to induce inflammatory injury in Kupffer cells (KCs) . After Aur treatment with gradient concentration, flow cytometry, propidium iodide (PI) staining, and Hoechst 33342 staining were used to detect the apoptotic level of KCs, and an enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of inflammatory factors, including Interleukin-1β (IL-1β), Interleukin-18 (IL-18), and tumor necrosis factor alpha (TNF-α). Western blot was used to detect the expression of toll-like receptor 4 (TLR4), myeloid differentiation protein-2 (MD2), MyD88, and p-P65. Aur was labeled with biotin, followed by a pull-down assay to detect its binding with MD2. Moreover, D-GalN/LPS was used to induce acute liver injury in mice , followed by Aur treatment by gavage. H&E staining was used to detect the pathological changes of liver tissue, an IF assay was used to detect the expression of MD2, Western blot was used to detect the expression of relevant proteins.

Results: Aur pretreatment could significantly inhibit LPS-induced KC injury, downregulate the apoptotic level, inhibit the expression of inflammatory factors, decrease the level of MDA, and downregulate the expression of MD2 in cells. Aur could inhibit the activation level of TLR4/MD2-NF-κB in a dose-dependent pattern, a high dose of Aur had a superior effect compared to low-dose Aur. In the case of MD2 deletion, the effects of Aur were suppressed. Additionally, pull-down and co-immunoprecipitation assays show that Aur can bind with the MD2 protein to inhibit the activation of TLR4/MD2-NF-κB. Results of mice experiments also showed that Aur could relieve liver injury, decrease the levels of ALT and AST, and simultaneously downregulate the levels of inflammatory factors in tissues and peripheral blood.

Conclusion: We found that Aur exerted an anti-inflammatory effect by directly targeting the MD2 protein, further inhibiting the expression of TLR4/MD2-NF-κB, thereby relieving acute liver injury. Therefore, Aur might be a potential inhibitor for MD2.
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http://dx.doi.org/10.3389/fphar.2020.570776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655772PMC
October 2020

Intra-arterial lidocaine for pain control after uterine artery embolization: a meta-analysis of randomized controlled trials.

J Matern Fetal Neonatal Med 2020 Nov 10:1-6. Epub 2020 Nov 10.

Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing, China.

Introduction: The efficacy of intra-arterial lidocaine for pain control of uterine artery embolization remains controversial. We conduct a systematic review and meta-analysis to explore the influence of intra-arterial lidocaine versus placebo on the postoperative pain intensity of uterine artery embolization.

Methods: We searched PubMed, EMbase, Web of science, EBSCO, and Cochrane library databases through April 2020 for randomized controlled trials (RCTs) assessing the effect of intra-arterial lidocaine versus placebo on pain control of uterine artery embolization. This meta-analysis is performed using the random-effect model.

Results: Three RCTs were included in the meta-analysis. Overall, compared with control group for uterine artery embolization, intra-arterial lidocaine was associated with substantially reduced pain scores at 4 h (SMD = -0.85; 95% CI = -1.31 to -0.38;  = .0003) and analgesic consumption (SMD = -0.84; 95% CI = -1.26 to -0.42;  < .0001), but has no obvious influence on pain scores at 7 h (SMD = -0.19; 95% CI = -0.63 to 0.25;  = .40) or pain scores at 24 h (SMD = -0.55; 95% CI = -1.25 to 0.16;  = .13).

Conclusions: Intra-arterial lidocaine is effective for pain control after uterine artery embolization.
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http://dx.doi.org/10.1080/14767058.2020.1847079DOI Listing
November 2020

Correlation between LncRNA-LINC00659 and clinical prognosis in gastric cancer and study on its biological mechanism.

J Cell Mol Med 2020 12 3;24(24):14467-14480. Epub 2020 Nov 3.

Department of Center Laboratory, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China.

Non-coding RNAs play important roles in tumorigenesis and tumour progression. In previous screening, lncRNA-LINC00659 (LINC00659) is highly expressed in gastric cancer; however, its role in gastric cancer has not been illustrated yet. In this study, the expression of LINC00659 was detected in cancer tissues and paracancerous tissues of patients with gastric cancer. As a result, LINC00659 expression was increased in gastric cancer tissues, which was closely associated with tumour stage and lymph node metastasis, but was not correlated with age, gender and tissue differentiation. Survival curve analysis showed that patients with low expression of LINC00659 harboured higher overall survival. In vitro, the level of LINC00659 was increased in gastric cancer cells. Afterwards, the expression of LINC00659 was down-regulated in SGC-7901 and BGC-823 cells by plasmid-mediated si-LINC00659 transfection. Consequently, the cell invasion ability was weakened, the cell cycle was inhibited, and cell viability was also suppressed. Luciferase reporter gene assay and RNA pull-down assay showed that LINC00659 could bind to the transcription factor SUZ12, indicating that SUZ12 was a regulatory gene of LINC00659. The overexpression of SUZ12 could resist the roles of si-LINC00659. In this study, we found that LINC00659 was highly expressed in gastric cancer, which might be related to the regulation of cell proliferation and promotion of cell invasion. Transcription factor, SUZ12, was a regulator of LINC00659. Additionally, LINC00659 could regulate cell cycle and invasion of gastric cancer by promoting the expression of SUZ12.
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http://dx.doi.org/10.1111/jcmm.16069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754062PMC
December 2020

Metal-Free Intramolecular Aminophosphination of Allenes.

J Org Chem 2020 Dec 29;85(23):15686-15692. Epub 2020 Oct 29.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, People's Republic of China.

A metal-free intramolecular aminophosphination of sulfonamidoallenes with diarylphosphine oxides and TfO was developed. This method offers a general and practical procedure to construct valuable alkenylphosphine-substituted N-heterocycles via the bifunctionalization reaction of allenes in good yields under mild conditions.
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http://dx.doi.org/10.1021/acs.joc.0c02169DOI Listing
December 2020

Mechanisms of the enhanced DDT removal from soils by earthworms: Identification of DDT degraders in drilosphere and non-drilosphere matrices.

J Hazard Mater 2021 02 23;404(Pt B):124006. Epub 2020 Sep 23.

Joint Institute for Environmental Research & Education, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China. Electronic address:

The remediation of soil contaminated by 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) remains an important issue in environmental research. Although our previous studies demonstrated that earthworms could enhance the degradation of DDT in soils, the underlying mechanisms and microorganisms involved in these transformation processes are still not clear. Here we studied the transformation of DDT in sterilized/non-sterilized drilosphere and non-drilosphere matrices and identified DDT degraders using the technique of DNA-stable isotope probing. The results show that DDT degradation in non-sterilized drilosphere was quicker than that in their non-drilosphere counterparts. Earthworms enhance DDT removal mainly by improving soil properties, thus stimulating indigenous microorganisms rather than abiotic degradation or tissue accumulating. Ten new genera, including Streptomyces, Streptacidiphilus, Dermacoccus, Brevibacterium, Bacillus, Virgibacillus, were identified as DDT ring cleavage degrading bacteria in the five matrices tested. Bacillus and Dermacoccus may also play vital roles in the dechlorination of DDTs as they were highly enriched during the incubations. The results of this study provide robust evidence for the application of earthworms in remediating soils polluted with DDT and highlight the importance of using combinations of cultivation-independent techniques together with process-based measurements to examine the function of microbes degrading organic pollutants in drilosphere matrices.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124006DOI Listing
February 2021

Antrodia camphorata polysaccharide resists 6-OHDA-induced dopaminergic neuronal damage by inhibiting ROS-NLRP3 activation.

Brain Behav 2020 11 9;10(11):e01824. Epub 2020 Sep 9.

Department of Neurology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China.

Introduction: Parkinson's disease (PD) is a common degenerative disease of the central nervous system (CNS). The main pathological change is the apoptosis of dopaminergic neurons in the substantia nigra pars compacta (SNPc), thereby leading to dopamine reduction in nigral striatum. 6-Hydroxydopamine (6-OHDA), a neurotoxic substance, mediates apoptosis of dopaminergic neurons and causes Parkinson-like symptoms in mice.

Methods: Our team previously found that Antrodia camphorata polysaccharide (ACP) exerted a good behavioral improvement effect on the PD mouse model established by 6-OHDA; however, the mechanism remains unknown. Therefore, in this study, we focused on ROS-NLRP3 signal to investigate the mechanism of 6-OHDA-induced apoptosis of dopaminergic neurons MES23.5 and the protective effects of ACP on dopaminergic neurons.

Result: 6-OHDA could further activate the expression of inflammasome NLRP3 by inducing ROS, thereby resulting in apoptosis of MES23.5 cells. ACP could inhibit the expression of ROS-NLRP3 induced by 6-OHDA, exerting a protective role in MES23.5 cells. Animal experiments also confirmed that ACP intervention could reduce the activation level of ROS-NLRP3 in the substantia nigra-striatum and improve the exercise capacity of PD mice.

Conclusion: Our study validated that 6-OHDA could induce apoptosis of dopaminergic neurons via ROS-NLRP3 activation. ACP could inhibit this signal and protect dopaminergic neurons, which might be promising in research of PD therapeutics.
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http://dx.doi.org/10.1002/brb3.1824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667329PMC
November 2020

Antrodia camphorata polysaccharide resists 6-OHDA-induced dopaminergic neuronal damage by inhibiting ROS-NLRP3 activation.

Brain Behav 2020 11 9;10(11):e01824. Epub 2020 Sep 9.

Department of Neurology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China.

Introduction: Parkinson's disease (PD) is a common degenerative disease of the central nervous system (CNS). The main pathological change is the apoptosis of dopaminergic neurons in the substantia nigra pars compacta (SNPc), thereby leading to dopamine reduction in nigral striatum. 6-Hydroxydopamine (6-OHDA), a neurotoxic substance, mediates apoptosis of dopaminergic neurons and causes Parkinson-like symptoms in mice.

Methods: Our team previously found that Antrodia camphorata polysaccharide (ACP) exerted a good behavioral improvement effect on the PD mouse model established by 6-OHDA; however, the mechanism remains unknown. Therefore, in this study, we focused on ROS-NLRP3 signal to investigate the mechanism of 6-OHDA-induced apoptosis of dopaminergic neurons MES23.5 and the protective effects of ACP on dopaminergic neurons.

Result: 6-OHDA could further activate the expression of inflammasome NLRP3 by inducing ROS, thereby resulting in apoptosis of MES23.5 cells. ACP could inhibit the expression of ROS-NLRP3 induced by 6-OHDA, exerting a protective role in MES23.5 cells. Animal experiments also confirmed that ACP intervention could reduce the activation level of ROS-NLRP3 in the substantia nigra-striatum and improve the exercise capacity of PD mice.

Conclusion: Our study validated that 6-OHDA could induce apoptosis of dopaminergic neurons via ROS-NLRP3 activation. ACP could inhibit this signal and protect dopaminergic neurons, which might be promising in research of PD therapeutics.
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http://dx.doi.org/10.1002/brb3.1824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667329PMC
November 2020