Publications by authors named "Wenting Xu"

84 Publications

Can Watching Online Videos Be Addictive? A Qualitative Exploration of Online Video Watching among Chinese Young Adults.

Int J Environ Res Public Health 2021 Jul 6;18(14). Epub 2021 Jul 6.

Department of Psychology, School of Education, Soochow University, Suzhou 215123, China.

Watching online videos (including short-form videos) has become the most popular leisure activity in China. However, a few studies have reported the potential negative effects of online video watching behaviors (including the potential for 'addiction') among a minority of individuals. The present study investigated online video watching behaviors, motivational factors for watching online videos, and potentially addictive indicators of watching online videos. Semi-structured interviews were conducted among 20 young Chinese adults. Qualitative data were analyzed using thematic analysis. Eight themes were identified comprising: (i) content is key; (ii) types of online video watching; (iii) platform function hooks; (iv) personal interests; (v) watching becoming habitual; (vi) social interaction needs; (vii) reassurance needs; and (viii) addiction-like symptoms. Specific video content (e.g., mukbang, pornography), platform-driven continuous watching, and short-form videos were perceived by some participants as being potentially addictive. Specific features or content on Chinese online video platforms (e.g., 'Danmu' scrolling comments) need further investigation. Future studies should explore users' addictive-like behaviors in relation to specific types of online video content and their social interaction on these platforms.
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http://dx.doi.org/10.3390/ijerph18147247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306552PMC
July 2021

Characterization of the complete chloroplast genome of Turcz.

Mitochondrial DNA B Resour 2021 Jun 14;6(7):1971-1972. Epub 2021 Jun 14.

Department of Chinese medicine, Hainan Maternal and Children's Medical Center, Haikou, P.R. China.

Turcz. is a perennial herb belonging to the family Papaveraceae. Its chloroplast genome was sequenced and characterized. The cp genome of is 167,629 bp long with a GC content of 36.52%. A total of 144 genes were identified in this cp genome, including 79 protein-coding genes, 31 tRNAs and four rRNAs. A phylogenetic tree based on the complete nucleic acid sequence indicated that was classified into Corydaleae and had a close relationship with .
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http://dx.doi.org/10.1080/23802359.2021.1925984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204951PMC
June 2021

HOXD Antisense Growth-Associated Long Noncoding RNA Promotes Triple-Negative Breast Cancer Progression by Activating Wnt Signaling Pathway.

J Breast Cancer 2021 Jun 28;24(3):315-329. Epub 2021 Apr 28.

Department of Breast Surgery, Zhengxing Hospital, Zhangzhou, China.

Purpose: Triple-negative breast cancer (TNBC) is the most lethal subtype of breast cancer owing to high heterogeneity, aggressive nature, and lack of treatment options, which has a substantial deleterious effect on patients' lives. HOXD antisense growth-associated long noncoding RNA (lncRNA) (HAGLR) plays tumor-promoting roles in many cancers. In this study, we aimed to explore the role of HAGLR in TNBC.

Methods: Quantitative real-time polymerase chain reaction assays were used to examine the expression of RNAs. Functional experiments were conducted to test the biological behavior of TNBC cells. Moreover, MS2-RNA immunoprecipitation, luciferase reporter, and RNA pull-down assays were conducted to verify the binding relationship between HAGLR, microRNA-143-5p (miR-143-5p), and serine- and arginine-rich splicing factor 1 (SRSF1).

Results: HAGLR was found to be highly expressed in TNBC tissues and cells, and inhibiting HAGLR suppressed cell proliferation, migration, and invasion and promoted cell apoptosis in TNBC. Meanwhile, miR-93-5p was shown to bind to HAGLR and SRSF1. In addition, SRSF1 plays an oncogenic role in TNBC. Importantly, HAGLR could activate the Wnt signaling pathway by sponging miR-93-5p to upregulate SRSF1; thus, accelerating TNBC progression.

Conclusion: HAGLR could promote the progression of TNBC through the miR-93-5p/SRSF1 axis to activate the Wnt signaling pathway.
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http://dx.doi.org/10.4048/jbc.2021.24.e24DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250102PMC
June 2021

MET and FASN as Prognostic Biomarkers of Triple Negative Breast Cancer: A Systematic Evidence Landscape of Clinical Study.

Front Oncol 2021 27;11:604801. Epub 2021 May 27.

Hunan University of Medicine, Huaihua, China.

Background: To know the expression of Mesenchymal-Epithelial Transition factor (MET) and Fatty Acid Synthase (FASN) in Triple Negative Breast Cancer (TNBC) patients, as well as its relationship with clinical pathological characteristic and prognosis.

Methods: we used immunohistochemistry staining to detect the expression of MET and FASN for those 218 TNBC patients, and analyze their relationship with the clinical pathological characteristic and prognosis.

Results: 130 and 65 out of 218 TNBC patients were positive for MET in the cancer and adjacent tissues respectively. 142 and 30 out of 218 TNBC patients were positive for FASN in the cancer and adjacent tissues respectively. Positive expression of MET and FASN were significantly correlated with lymph node metastasis, pathological TNM, and pathological Stage. In addition, the positive expression of MET and FASN were correlated with recurrence and metastasis. The combined use of MET and FASN can better predict the survival condition.

Conclusions: Our results indicated that MET and FASN showed good predictive ability for TNBC. Combined use of MET and FASN were recommended in order to make a more accurate prognosis for TNBC.
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http://dx.doi.org/10.3389/fonc.2021.604801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190390PMC
May 2021

Activated Carbon Nanotube Fiber Fabric as a High-Performance Flexible Electrode for Solid-State Supercapacitors.

ACS Appl Mater Interfaces 2021 Jun 11;13(24):28433-28441. Epub 2021 Jun 11.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, China.

Owing to their features of excellent mechanical flexibility, high conductivity, and light weight, carbon-based fiber fabrics (CBFFs) are highly attractive as flexible electrodes for flexible solid-state supercapacitors (SCs). However, the achieved areal capacitance of most CBFFs is still unsatisfactory. Carbon nanotube fiber fabric (CNTFF) is a new kind of CBFF and could provide a potential alternative to high-performance flexible electrodes. Herein, we report the activation of CNTFF using a facile thermal oxidation and acid treatment process. The activated CNTFF shows an exceptional combination of large areal capacitance (1988 mF cm at 2 mA cm), excellent rate performance (45% capacitance reservation at 100 mA cm), and outstanding cycle life (only 3% capacitance decay after 10,000 cycles). The constructed solid-state SC reaches a maximum energy density of 143 μWh cm at 1000 μW cm and a maximum power density of 30,600 μW cm at 82 μWh cm. Additionally, this device possesses good rate performance along with superb cycle stability and excellent mechanical flexibility under various bending conditions. Our present work therefore offers a new opportunity in developing high-performance flexible electrodes for flexible energy storage.
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http://dx.doi.org/10.1021/acsami.1c02758DOI Listing
June 2021

The complete chloroplast genome of Nakai.

Mitochondrial DNA B Resour 2021 May 27;6(6):1779-1780. Epub 2021 May 27.

Department of Critical Medicine, Hainan Maternal and Children's Medical Center, Haikou, P.R. China.

Nakai is a fern plant with important evolutionary and medicinal values. Herein, we assembled the complete chloroplast genome of by next-generation sequencing technology. The complete chloroplast genome of was 153,355 bp in length, and the GC content was 42.86%; the genome consisted of a pair of inverted repeats (IRs, 23,470 bp), a small single copy region (SSC, 21,570 bp) and a large single copy region (LSC, 84,854 bp). The genome contained 111 genes, namely, 73 protein-coding genes, 34 tRNA genes and four rRNA genes. The phylogenetic analysis suggested that both and from Dryopteridaceae were most closely related to from Polypodiaceae.
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http://dx.doi.org/10.1080/23802359.2021.1933634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168769PMC
May 2021

Role of Ferroptosis in Lung Diseases.

J Inflamm Res 2021 20;14:2079-2090. Epub 2021 May 20.

Department of Anesthesiology, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui, 236000, People's Republic of China.

Ferroptosis is a new type of programmed cell death characterized by intracellular iron accumulation and lipid peroxidation that leads to oxidative stress and cell death. The metabolism of iron, lipids, and amino acids and multiple signalling pathways precisely regulate the process of ferroptosis. Emerging evidence has demonstrated that ferroptosis participates in the occurrence and progression of various pathological conditions and diseases, such as infections, neurodegeneration, tissue ischaemia-reperfusion injury and immune diseases. Recent studies have also indicated that ferroptosis plays a critical role in the pathogenesis of acute lung injury, chronic obstructive pulmonary disease, pulmonary fibrosis, pulmonary infection and asthma. Herein, we summarize the latest knowledge on the regulatory mechanism of ferroptosis and its association with iron, lipid and amino acid metabolism as well as several signalling pathways. Furthermore, we review the contribution of ferroptosis to the pathogenesis of lung diseases and discuss ferroptosis as a novel therapeutic target for various lung diseases.
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http://dx.doi.org/10.2147/JIR.S307081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144020PMC
May 2021

H. pylori infection induces CXCL8 expression and promotes gastric cancer progress through downregulating KLF4.

Mol Carcinog 2021 Aug 26;60(8):524-537. Epub 2021 May 26.

Department of Physiopathology, Anhui Medical University, Hefei, Anhui, China.

Tumour-derived CXCL8 facilitates the movement of myeloid-derived suppressor cells, which are able to restrain antitumour immune responses to the tumour microenvironment. Kruppel-like factor 4 (KLF4) is a potential tumour suppressor in gastric cancer (GC). However, knowledge regarding correlations between KLF4 and CXCL8 in GC is limited. We use cellular and molecular biological methods to assess whether these two factors interact in GC. Expression CXCL8 and KLF4 was altered in human GC tissues compared to normal gastric tissues in opposite ways. Additionally, cytotoxin-associated gene A protein (CagA) gene transduction or Helicobacter pylori (H. pylori) infection upregulated CXCL8 expression. Knockdown of KLF4 expression increased CXCL8 protein and RNA expression, whereas its overexpression had the opposite effect. CXCL8-mediated enhancement of GC cell migration and proliferation was reversed by upregulation of KLF4 expression. Further mechanistic research revealed that KLF4 binds the CXCL8 promoter, suppressing CXCL8 transcription. Moreover, CXCL8 stimulation reduced KLF4 protein expression and promoted GC cell proliferation and migration, eventually promoting neoplasm growth in vivo. Together, our findings demonstrate that CagA promotes CXCL8 and inhibits KLF4. CXCL8 is a decisive downstream target gene of KLF4, and KLF4 negatively regulates CXCL8 in GC. Furthermore, CXCL8's negative regulation of KLF4 in vivo and in vitro, indicates that CagA may downregulate KLF4 by inducing CXCL8 expression, low expression of KLF4 further promotes that of CXCL8, forming a vicious circle in GC. Targeted KLF4 activation might improve the immunosuppressive microenvironment through direct negative regulation of CXCL8, providing a new potential target to strengthen the efficacy of immunotherapy in GC patients.
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http://dx.doi.org/10.1002/mc.23309DOI Listing
August 2021

Enhanced tensile and electrochemical performance of MXene/CNT hierarchical film.

Nanotechnology 2021 Jun 11;32(35). Epub 2021 Jun 11.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, People's Republic of China.

Nowadays, it is highly desirable to achieve high strength, flexibility and electrochemical performance for supercapacitor electrodes simultaneously. Herein, few-layer MXene flakes are assembled into free-standing films by facile vacuum-filtration method, in which hydrophilic-functionalized carbon nanotubes (CNTs) are further incorporated. The morphology of MXene/CNT composite films evolves from compact to 'CNT in MXene' to laminar to 'MXene in CNT' and finally to separate structures when increasing the CNT weight percentage. Among them, the laminar structure in which thin MXene and CNT layers are stacked alternately is demonstrated to be the best. The laminar MXene/CNT film possesses much higher strength, elongation and specific capacitance than MXene film due to the engineered porosity, good interaction between MXene flakes and CNTs, and proper CNTs' distribution. As a result, high specific capacitance of 423.4 F gat 1 A gand capacitance retention of nearly 60% at 10 A gare accomplished. Moreover, the composite film is flexible and withstands bending up to 180°, indicating that the proposed laminar MXene/CNT composite film is a superb candidate for flexible supercapacitors.
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http://dx.doi.org/10.1088/1361-6528/ac04cfDOI Listing
June 2021

Increasing Stability of SnO-Based Perovskite Solar Cells by Introducing an Anionic Conjugated Polyelectrolyte for Interfacial Adjustment.

ACS Appl Mater Interfaces 2021 Jun 19;13(21):24575-24581. Epub 2021 May 19.

Jiangsu National Synergistic Innovation Centre for Advanced Materials (SICAM), Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, P.R. China.

Despite the fact that power conversion efficiency (PCE) has been greatly improved in recent years, perovskite solar cells (PSCs) need to overcome some challenges, like stability, for the commercial application. Herein, an anionic conjugated polyelectrolyte, sulfonic-containing polyfluorene (abbreviated to SPF), has been developed to modify the interface between the electron-transporting layer (ETL) SnO and the optoelectronic active layer MAPbI in the cells. After 40 days of storage in atmospheric environment in the dark with exposure to a controlled humidity of about 10%, PCE of the SPF-modified cells with the structure of ITO/SnO/SPF/MAPbI/spiro-OMeTAD/Au still remained 94% of the initial value. In contrast, the control cell without SPF only remained 31.1% of its initial efficiency after 29 days. The main reason for the stability enhancement is the adjustment of interfacial energy level, the crystallinity enhancement, and the removal of the interfacial defect of the perovskite layer by introducing the hydrophobic and smooth SPF interfacial layer. Deep electrical study on the PSCs discloses that the cell has low carrier transfer resistance, low leakage current density, and minor interfacial charge accumulation. What's more, the short-circuit current density is improved, and PCE of 20.47% is achieved.
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http://dx.doi.org/10.1021/acsami.1c00410DOI Listing
June 2021

Individualized protocol for radiation and contrast medium dose reduction in one-stop assessment for kidney transplantation patients.

Eur J Radiol 2021 Jul 7;140:109757. Epub 2021 May 7.

Department of Radiology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. Electronic address:

Objective: To compare image quality, radiation, and contrast medium (CM) doses between individualized and conventional scan protocols in combined coronary CT angiography (CCTA) and iliac artery CTA for kidney transplantation patients.

Methods: 148 patients needing assessment for coronary and iliac arteries before kidney transplantation were prospectively enrolled and randomly divided into the conventional and individualized groups. All patients underwent one-stop combined scans on a 256-row CT scanner with automatic tube current modulation, 50 % pre-ASIR-V to control radiation dose. CCTA was performed first using one heartbeat axial scan mode with bolus tracking technique and iliac CTA was performed 3 s after CCTA using a spiral scan. The conventional group (n = 72) used the standard protocol: 100 kVp, 60 mL of 350 mgI/mL CM at 4.5 mL/s flow rate. The individualized group (n = 76) used a body-mass-index (BMI)-dependent protocol: kVp: 80 (BMI < 24) and 100 (BMI ≥ 24) and CM: 19 mgI/kg (BMI < 18); 21 mgI/kg (18 ≤ BMI < 24); and 22 mgI/kg (BMI ≥ 24). Image quality radiation and CM doses of the two groups were compared.

Results: There was no significant difference in patient demographic data. Compared with the conventional group, the individualized group reduced contrast flow rate (in mL/s) by 14.4 % (3.85 ± 0.72 vs. 4.5), contrast dose (in mL) by 35.8 % (38.53 ± 7.18 vs. 60) and radiation dose (in mSv) by 34.3 % (4.30 ± 1.73 vs. 6.54 ± 1.45). The individualized group had significantly higher subjective image quality score (P < 0.05), lower noise (17.30 ± 4.97 HU vs. 19.13 ± 4.73 HU, P = 0.02) and higher signal-to-noise ratio (22.09 ± 7.41 vs. 19.55 ± 6.18, P = 0.03) for the three main vessels in CCTA compared with the conventional group. There were no differences in both subjective scores and objective measurements in iliac artery CTA between the two groups.

Conclusion: The individualized scanning protocol in the one-stop assessment of coronary and iliac arteries before kidney transplantation significantly reduces both radiation and CM doses while maintaining image quality in iliac artery CTA and providing better coronary artery images in CCTA.
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http://dx.doi.org/10.1016/j.ejrad.2021.109757DOI Listing
July 2021

An Event-Specific Real-Time PCR Method for Measuring Transgenic Lysozyme Goat Content in Trace Samples.

Foods 2021 Apr 23;10(5). Epub 2021 Apr 23.

Joint International Research Laboratory, Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Lysozymes are used in sterilisation, antisepsis, dairy additives, inflammation, and cancer. One transgenic goat line expressing high levels of human lysozyme (hLZ) in goat milk has been developed in China. Herein, we established an event-specific real-time polymerase chain reaction (real-time PCR) method to detect the transgenic hLZ goat line. The developed method has high specificity, sensitivity and accuracy, and a wide quantitative dynamic range. The limit of detection and limit of quantification was 5 and 10 copies per reaction, respectively. The practical sample analysis results showed that the method could identify and quantify transgenic lysozyme content in trace samples in routine lab analyses. Furthermore, the potential applicability in risk assessment, such as molecular characterisation and gene horizontal transfer, was confirmed. We believe that this method is suitable for the detection of transgenic hLZ goat line and its derivate.
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http://dx.doi.org/10.3390/foods10050925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146569PMC
April 2021

Age-related trabecular bone loss is associated with a decline in serum Galectin-1 level.

BMC Musculoskelet Disord 2021 Apr 27;22(1):394. Epub 2021 Apr 27.

Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, Jiangsu, China.

Background: Senile osteoporosis with age-related bone loss is diagnosed depending on radiographic changes of bone and bone mineral density (BMD) measurement. However, radiographic alterations are usually signs of medium-late stage osteoporosis. Therefore, biomarkers have been proposed as indicators of bone loss. In the current study, Galectin-1 (Gal-1) showed age-related decline in mice serum. The role of Gal-1 in osteoporosis has not been investigated so far. Hence, the current study illustrated the relationship of serum Gal-1 level with bone loss.

Methods: We employed 6- and 18-month-old mice to establish an animal model of age-related trabecular bone loss, whose bone density and microstructure were investigated by micro-CT. ELISA was used to measure the levels of Gal-1 in serum. The correlation analysis was performed to illustrate the relationship between serum Gal-1 levels and trabecular bone loss. In addition, immunohistochemistry was used to investigate the abundance of Gal-1 in bone marrow of mice. ELISA and western blot were performed to measure the secretion ability and protein expression of Gal-1 in bone marrow stromal cells (BMSC), hematopoietic stem cells (HSC) and myeloid progenitor (MP) respectively. Flow cytometry was used to measure BMSC number in bone marrow. Finally, male volunteers with age-related BMD decrease were recruited and the relationship between serum Gal-1 and BMD was analyzed.

Results: Gal-1 showed age-related decline in mice serum. Serum Gal-1 was positively associated with BV/TV of femur, tibia and L1 vertebrae in mice. BMSC secreted more Gal-1 compared with HSC and MP. BMSC number in bone marrow was significantly lower in aged mice compared with young mice. Significant attenuation of Gal-1 protein expression was observed in BMSC and HSC from aged mice compared with young mice. Further, we found a decline in serum Gal-1 levels in men with age-related BMD decrease. There was positive correlation between BMD and serum Gal-1 levels in these men.

Conclusions: Age-related trabecular bone loss is associated with a decline in serum Gal-1 level in mice and men. Our study suggested Gal-1 had great potential to be a biomarker for discovering BMSC senescence, diagnosing early osteoporosis and monitoring trabecular bone loss.
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http://dx.doi.org/10.1186/s12891-021-04272-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080405PMC
April 2021

The protease corin regulates electrolyte homeostasis in eccrine sweat glands.

PLoS Biol 2021 02 16;19(2):e3001090. Epub 2021 Feb 16.

Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Prevention, the First Affiliated Hospital, Soochow University, Suzhou, China.

Sweating is a basic skin function in body temperature control. In sweat glands, salt excretion and reabsorption are regulated to avoid electrolyte imbalance. To date, the mechanism underlying such regulation is not fully understood. Corin is a transmembrane protease that activates atrial natriuretic peptide (ANP), a cardiac hormone essential for normal blood volume and pressure. Here, we report an unexpected role of corin in sweat glands to promote sweat and salt excretion in regulating electrolyte homeostasis. In human and mouse eccrine sweat glands, corin and ANP are expressed in the luminal epithelial cells. In corin-deficient mice on normal- and high-salt diets, sweat and salt excretion is reduced. This phenotype is associated with enhanced epithelial sodium channel (ENaC) activity that mediates Na+ and water reabsorption. Treatment of amiloride, an ENaC inhibitor, normalizes sweat and salt excretion in corin-deficient mice. Moreover, treatment of aldosterone decreases sweat and salt excretion in wild-type (WT), but not corin-deficient, mice. These results reveal an important regulatory function of corin in eccrine sweat glands to promote sweat and salt excretion.
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http://dx.doi.org/10.1371/journal.pbio.3001090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909636PMC
February 2021

induces epithelial-mesenchymal transition in gastric carcinogenesis via the AKT/GSK3β signaling pathway.

Oncol Lett 2021 Feb 4;21(2):165. Epub 2021 Jan 4.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.

() is a main risk factor for gastric cancer (GC). Epithelial-mesenchymal transition (EMT) is involved in the development and progression of -associated GC. However, the exact molecular mechanism of this process remains unclear. The AKT/GSK3β signaling pathway has been demonstrated to promote EMT in several types of cancer. The present study investigated whether infection induced EMT, and promoted the development and metastasis of cancer in the normal gastric mucosa, and whether this process was dependent on AKT activation. The expression levels of the EMT-associated proteins, including E-cadherin and N-cadherin, were determined in 165 gastric mucosal samples of different disease stages by immunohistochemical analysis. The expression levels of E-cadherin, N-cadherin, AKT, phosphorylated (p-)AKT (Ser473), GSK3β and p-GSK3β (Ser9) were further determined in -infected Mongolian gerbil gastric tissues and cells co-cultured with by immunohistochemical analysis and western blotting. The results indicated that the expression levels of the epithelial marker E-cadherin were decreased, whereas the expression levels of the mesenchymal marker N-cadherin were increased during gastric carcinogenesis. Their expression levels were associated with infection. Furthermore, infection resulted in downregulation of E-cadherin expression and upregulation of N-cadherin expression in Mongolian gerbils and GES-1 cells. In addition, an investigation of the associated mechanism of action revealed that p-AKT (Ser473) and p-GSK3β (Ser9) were activated in GES-1 cells following co-culture with . Furthermore, following pretreatment of the cells with the AKT inhibitor VIII, the expression levels of E-cadherin, N-cadherin, p-AKT and p-GSK3β did not show significant differences between GES-1 cells that were co-cultured with or without . The levels of p-AKT and p-GSK3β were increased in -infected Mongolian gerbils. In conclusion, the present study demonstrated that infection activated AKT and resulted in the phosphorylation and inactivation of GSK3β, which in turn promoted early stage EMT. These effects were AKT-dependent. This mechanism may serve as a prerequisite for GC development.
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http://dx.doi.org/10.3892/ol.2021.12426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798028PMC
February 2021

Universal LNA Probe-Mediated Multiplex Droplet Digital Polymerase Chain Reaction for Ultrasensitive and Accurate Quantitative Analysis of Genetically Modified Organisms.

J Agric Food Chem 2021 Feb 2;69(5):1705-1713. Epub 2021 Feb 2.

Development Center of Science and Technology, Ministry of Agriculture of People's Republic of China, Beijing 100025, China.

Multiplex and high-throughput assays are becoming the main trends in the development of new nucleic acid detection and quantification methods, such as those for genetically modified organism (GMO) analysis. Here, we report a novel universal LNA probe-mediated droplet digital polymerase chain reaction (PCR) method (ULNA-ddPCR) for multiple DNA target quantification in GMOs. In ULNA-ddPCR, only one universal LNA probe is used for multiple DNA targets instead of using one to one TaqMan probe. The specificity, sensitivity, dynamic range, and accuracy of the ULNA-ddPCR method are determined by employing GM rice analysis as an example. Simplex and triplex ULNA-ddPCR assays for three GM rice events, T2A-1, T1C-19, and G6H1, are established and evaluated. All results indicate that the developed simplex and triplex ULNA-ddPCR assays are suitable for quantitative analysis of GM rice events with high sensitivity, accuracy, and low cost. The ULNA-ddPCR method also has the potential for multiple DNA target quantification in other research fields.
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http://dx.doi.org/10.1021/acs.jafc.0c06433DOI Listing
February 2021

A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 Enhancement of Endomitosis in Fish.

Front Bioeng Biotechnol 2020 13;8:606496. Epub 2021 Jan 13.

State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, China.

Previous studies have suggested that 1,9-Pyrazoloanthrone, known as SP600125, can induce cell polyploidization. However, what is the phase of cell cycle arrest caused by SP600125 and the underlying regulation is still an interesting issue to be further addressed. Research in this article shows that SP600125 can block cell cycle progression at the prometaphase of mitosis and cause endomitosis. It is suggested that enhancement of the p53 signaling pathway and weakening of the spindle assembly checkpoint are associated with the SP600125-induced cell cycle arrest. Using preliminary SP600125 treatment, the samples of the cultured fish cells and the fish tissues display a great number of chromosome splitting phases. Summarily, SP600125 can provide a new protocol of chromosomes preparation for karyotype analysis owing to its interference with prometaphase of mitosis.
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http://dx.doi.org/10.3389/fbioe.2020.606496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838586PMC
January 2021

Acupuncture Ameliorates Neuronal Cell Death, Inflammation, and Ferroptosis and Downregulated miR-23a-3p After Intracerebral Hemorrhage in Rats.

J Mol Neurosci 2021 Jan 5. Epub 2021 Jan 5.

First Affiliated Hospital, Heilongjiang University of Chinese Medicine, No. 26, Heping Road, Xiangfang District, Harbin, 150040, China.

Baihui-penetrating-Qubin acupuncture is frequently used to treat intracerebral hemorrhage (ICH) in China. Acupuncture affects multiple microRNAs in diseases. MicroRNA-23a-3p (miR-23a-3p) has been demonstrated to be up-regulated in ICH patients. Herein, the effect of Baihui-penetrating-Qubin acupuncture on miR-23a-3p expression after ICH and the role of miR-23a-3p in ICH were discussed. A rat model of ICH was induced by infusing autologous blood into caudate nucleus. Acupuncture was performed after ICH once a day for 30 min. After 3 consecutive days of acupuncture, the neurobehavioral function, brain edema, neuronal cell death, inflammation, ferroptosis, nuclear factor E2-like 2 (NFE2L2) signaling and miR-23a-3p levels in brain tissues were analyzed. Additionally, antagomiR-23a-3p was injected into rats 3 days prior to ICH modeling to analyze the function of miR-23a-3p in neuronal cell death, inflammation, ferroptosis, and NFE2L2 signaling. Acupuncture relieved the ICH-induced neurological function deficits, increases in brain water content and Fluoro-Jade B (FJB)-positive cells and release of proinflammatory cytokines. Acupuncture also alleviated ferroptosis and decreased miR-23a-3p expression, as evidenced by the increased NFE2L2 nuclear translocation and expressions of heme oxygenase-1 and glutathione peroxidase 4 and the decreased iron and malondialdehyde contents and reactive oxygen species accumulation. Additionally, antagomiR-23a-3p inhibited the ICH-induced increases in FJB-positive cells, release of proinflammatory cytokines, ferroptosis, and promoted NFE2L2 activation. Notably, the binding site of miR-23a-3p existed in NFE2L2. Taken together, acupuncture may alleviate the neuronal cell death, inflammation, and ferroptosis after ICH by down-regulating miR-23a-3p. This study provides a potential mechanism underlying the Baihui-penetrating-Qubin acupuncture improving the early injury after ICH.
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http://dx.doi.org/10.1007/s12031-020-01770-xDOI Listing
January 2021

USP15 Deubiquitinates CARD9 to Downregulate C-Type Lectin Receptor-Mediated Signaling.

Immunohorizons 2020 10 22;4(10):670-678. Epub 2020 Oct 22.

Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114;

Posttranslational modifications are efficient means to rapidly regulate protein function in response to a stimulus. Although ubiquitination events and the E3 ubiquitin ligases involved are increasingly characterized in many signaling pathways, their regulation by deubiquitinating enzymes remains less understood. The C-type lectin receptor (CLR) signaling adaptor CARD9 was previously reported to be activated via TRIM62-mediated ubiquitination. In this study, we identify the deubiquitinase USP15 as a novel regulator of CARD9, demonstrating that USP15 constitutively associates with CARD9 and removes TRIM62-deposited ubiquitin marks. Furthermore, USP15 knockdown and knockout specifically enhance CARD9-dependent CLR signaling in both mouse and human immune cells. Altogether, our study identifies a novel regulator of innate immune signaling and provides a blueprint for the identification of additional deubiquitinases that are likely to control these processes.
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http://dx.doi.org/10.4049/immunohorizons.2000036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758836PMC
October 2020

Anti-inflammatory activities of puerarin in high-fat diet-fed rats with streptozotocin-induced gestational diabetes mellitus.

Mol Biol Rep 2020 Oct 18;47(10):7537-7546. Epub 2020 Sep 18.

Department of Reproduction, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhang jiagang, Suzhou, Jiangsu, China.

To investigate the effect of puerarin on insulin resistance and inflammation in rats with gestational diabetes mellitus (GDM). Gestational diabetic model rats were established by intraperitoneal injection of streptozotocin (25 mg/kg) combined with high-fat feeding and were randomly assigned to three groups: the control group, the GDM group, and the puerarin-treated group. Puerarin was intragastrically administered to rats daily until the offspring were born. The rats in both the GDM group and control group were administered the same volume of normal saline. Serum total cholesterol, triglycerides, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in all groups of rats were measured. Haematoxylin and eosin staining was used to evaluate morphological changes in the liver, pancreas, and adipose tissues around the reproductive organs. Western blotting was carried out to measure the protein expression of IRS-1 and inflammatory factors, including TNF-α, TLR4, MyD88 and phosphorylated NF-κB, in the adipose tissues around the reproductive organs. Puerarin had preventive effects on GDM-induced pathological changes and ameliorated glucose and lipid metabolism disorders in GDM rats. Puerarin upregulated IRS-1 expression and decreased the protein expression of TNF-α, TLR4, and MyD88 as well as the levels of phosphorylated NF-κB in adipose tissues around the reproductive organs in GDM rats. This study indicated that puerarin exerts anti-inflammatory effects by downregulating the important TLR4/MyD88/NF-κB inflammatory signalling pathway. Therefore, puerarin can decrease the expression of TNF-α and ameliorate insulin resistance in GDM rats, suggesting the potential efficacy of puerarin in GDM treatment.
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http://dx.doi.org/10.1007/s11033-020-05816-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588390PMC
October 2020

Safety and immunogenicity of an alum-adjuvanted whole-virion H7N9 influenza vaccine: a randomized, blinded, clinical trial.

Clin Microbiol Infect 2020 Jul 29. Epub 2020 Jul 29.

Henan Province Centre for Disease Control and Prevention, Centre of Vaccine Clinical Research, China. Electronic address:

Objectives: A case of H7N9 influenza virus infection was first identified in China in 2013. This virus is considered to have high pandemic potential. Here we developed an H7N9 influenza vaccine containing an aluminium adjuvant and evaluated the safety and immunogenicity of the vaccine.

Methods: From October 2017 through August 2018 we conducted a randomized, double-blinded, single-centre phase I clinical trial in China among 360 participants aged ≥12 years. All participants received two doses of the vaccine (7.5, 15 or 30 μg haemagglutinin antigen) or placebo at an interval of 21 days. Adverse event data were collected for 30 days after vaccination. Serum samples were collected on days 0, 21 and 42 for the haemagglutinin inhibition (HI) antibody assay.

Results: A total of 347 participants (347/360, 96.4%) completed the study. The proportions of vaccine-related adverse events after one injection were 56.7% (34/60) in the 7.5-μg group, 86.7% (52/60) in the 15-μg group and 86.7% (52/60) in the 30-μg group. The proportions of adverse events after two injections were less than those reported after the first dose. None of the serious adverse events were related to the vaccine. After receiving two doses of the 7.5-μg vaccine, the proportion of participants achieving an HI titre of ≥40 was 98.2% (55/56, 95%CI 72.3~100.0%), with a geometric mean titre (GMT) of 192.6 (95%CI 162.9~227.8).

Conclusions: The alum-adjuvanted H7N9 whole-virion inactivated vaccine was safe and strongly immunogenic in a population aged ≥12 years.
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http://dx.doi.org/10.1016/j.cmi.2020.07.033DOI Listing
July 2020

Clinical effects of Shou-Wu Jiang-Qi Decoction combined acupuncture on the treatment of Polycystic Ovarian Syndrome with kidney deficiency, phlegm and blood stasisness: Study protocol clinical trial (SPIRIT Compliant).

Medicine (Baltimore) 2020 Mar;99(12):e19045

Department of Reproduction, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang, Suzhou, Jiangsu, China.

Background: Polycystic ovary syndrome (PCOS) is a female endocrine disease with a high incidence. At present, drug treatment is still the main therapeutic strategy for PCOS. Traditional Chinese medicine has a long history in the treatment of menstrual disorders. Shouwu Jiangqi Decoction (SWJQD) is a traditional herbal medicine prescribed in a clinical setting as a remedy for PCOS. Acupuncture also plays an important role in regulating the menstrual cycle and treating PCOS. This study aims to examine the efficacy and safety of the combination of SWJQD and acupuncture in the treatment of PCOS.

Methods: This randomized controlled trial will be conducted with a total of 81 participants diagnosed with PCOS. The participants will be randomly divided into 3 treatment groups: group A will receive SWJQD combined with acupuncture; group B, SWJQD combined with sham acupuncture; and group C, metformin. Each treatment will last 3 months. The primary outcomes include the Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index and the Oral Glucose Tolerance Test. The secondary outcome measures include sex hormone levels, body mass index, ovulation rate, clinical pregnancy rate, and complete genome sequencing data. Adverse events will be recorded during the intervention and follow-up.

Results: This study will investigate whether the combination of SWJQD and acupuncture can alleviate the clinical symptoms and improve insulin resistance in patients with PCOS. The results of this study are expected to provide clinical evidence for the application of the combination of SWJQD and acupuncture in patients with PCOS.

Trial Registration: Chinese Clinical Trial Registry: ChiCTR1900028106, ChiMCT1900002826 (registered on December 12, 2019).
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http://dx.doi.org/10.1097/MD.0000000000019045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220733PMC
March 2020

Identification of the Active Constituents and Significant Pathways of Cangfu Daotan Decoction for the Treatment of PCOS Based on Network Pharmacology.

Evid Based Complement Alternat Med 2020 21;2020:4086864. Epub 2020 Feb 21.

Department of Reproduction, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang, Suzhou, Jiangsu, China.

Background: Polycystic ovary syndrome (PCOS) is the most common female endocrine disease. Cangfu Daotan Decoction (CDD) can effectively relieve the clinical symptoms of PCOS patients.

Methods: To explore the active ingredients and related pathways of CDD for treating PCOS, a network pharmacology-based analysis was carried out. The active ingredients of CDD and their potential targets were obtained from the TCM system pharmacology analysis platform. The obtained PCOS-related genes from OMIM and GeneCards were imported to establish protein-protein interaction networks in STRING. Finally, GO analysis and significant pathway analysis were conducted with the RStudio (Bioconductor) database.

Results: A total of 111 active compounds were obtained from 1433 ingredients present in the CDD, related to 118 protein targets. In addition, 736 genes were found to be closely related to PCOS, of which 44 overlapped with CDD and were thus considered therapeutically relevant. Pathway enrichment analysis identified the AGE-RAGE signalling pathway in diabetic complications, endocrine resistance, the IL-17 signalling pathway, the prolactin signalling pathway, and the HIF-1 signalling pathway. Moreover, PI3K-Akt, insulin resistance, Toll-like receptor, MAPK, and AGE-RAGE were related to PCOS and treatment.

Conclusions: CDD can effectively improve the symptoms of PCOS, and our network pharmacological analysis lays the foundation for future clinical research.
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http://dx.doi.org/10.1155/2020/4086864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057008PMC
February 2020

Helicobacter pylori infection leads to KLF4 inactivation in gastric cancer through a TET1-mediated DNA methylation mechanism.

Cancer Med 2020 04 4;9(7):2551-2563. Epub 2020 Feb 4.

Department of Physiopathology, Anhui Medical University, Hefei, Anhui, China.

Krüppel-like factor 4 (KLF4) has a tumor suppressor role in the progression of gastric cancer (GC), and inhibition or loss of KLF4 expression was identified in GC. The aim of this study was to explore the new molecular mechanism of KLF4 inactivation in gastric cancer. Herein, we report that Helicobacter pylori infection or Cag pathogenicity island protein A (CagA) gene transduction resulted in KLF4 expression downregulation and promoted gastric epithelial cell and gastric cancel cell proliferation, migration, and colony formation. Mechanistically, we found that CagA gene transduction led to DNA methylation of the KLF4 promoter, an effect that was relevant to the significant downregulation of TET1 expression. Causally, knockdown of TET1 expression decreased KLF4 expression, whereas overexpression of TET1 had the opposite effect. Clinically, we found that KLF4 expression and the 5-hmC levels were lower in GC cells with H pylori infection than in GC cells without H pylori infection. Thus, our study not only sheds new light on how H pylori infection promotes the progression of GC but also elucidates a novel mechanism of KLF4 inactivation in GC pathogenesis. During pathogenesis, an alteration in the H pylori/CagA-TET1-KLF4 signaling pathway plays a critical role, suggesting that this pathway may be a prospective target for gastric carcinoma intervention and therapy.
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http://dx.doi.org/10.1002/cam4.2892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131848PMC
April 2020

Soil respiration of four forests along elevation gradient in northern subtropical China.

Ecol Evol 2019 Nov 6;9(22):12846-12857. Epub 2019 Nov 6.

State Key Laboratory of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences Beijing China.

Background And Aims: Soil respiration is the second-largest terrestrial carbon (C) flux, and soil temperature and soil moisture are the main drivers of temporal variation in soil respiration and its components. Here, we quantified the contribution of soil temperature, soil moisture, and their intersection on the variation in soil respiration and its components of the evergreen broad-leaved forests (EBF), mixed evergreen and deciduous broad-leaved forests (MF), deciduous broad-leaved forests (DBF), and subalpine coniferous forests (CF) along an elevation gradient.

Methods: We measured soil respiration of four types of forests along the elevation gradient in Shennongjia, Hubei China based on the trenching experiments. We parameterized the relationships between soil respiration and soil temperature, soil moisture, and quantified the intersection of temperature and moisture on soil respiration and its components.

Results: Total soil respiration ( ), heterotrophic respiration ( ), and autotrophic respiration ( ) were significantly correlated with soil temperature in all four forests. The value of soil respiration significantly differed among the four types of forest, and the was 3.06 for EBF, 3.75 for MF, 4.05 for DBF, and 4.49 for CF, respectively. The soil temperature explained 62%-81% of the variation in respiration, while soil temperature and soil moisture together explained 91%-97% of soil respiration variation for the four types of forests. The variation from the intersection of soil temperature and moisture were 12.1%-25.0% in R, 1.0%-7.0% in and 17.1%-19.6% in respectively.

Conclusions: Our results show that the temperature sensitivity ( ) of soil respiration increased with elevation. The intersection between soil temperature and soil moisture had strong effects on soil respiration, especially in . We demonstrated that the intersection effects between soil temperature and soil moisture on soil respiration were essential to understand the response of soil respiration and its components to climate change.
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http://dx.doi.org/10.1002/ece3.5762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875676PMC
November 2019

Electrochemical Synthesis of -2,3-Disubstituted Aziridines via Oxidative Dehydrogenative Intramolecular C(sp)-H Amination.

Org Lett 2019 12 20;21(23):9430-9433. Epub 2019 Nov 20.

Engineering Technology Research Center of Henan Province for Photo- and Electrochemical Catalysis, College of Chemistry and Pharmaceutical Engineering , Nanyang Normal University , Nanyang 473061 , China.

An electrochemical oxidative dehydrogenative C(sp)-H amination reaction to construct -2,3-disubstituted aziridines is reported, which avoids using external oxidants and generates only hydrogen gas as the byproduct. With KI as the mediator and electrolyte, the desired aziridines were prepared in good yields in an undivided cell at room temperature. The potential value of this mild electrochemical strategy was further highlighted by the gram-scale synthesis.
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http://dx.doi.org/10.1021/acs.orglett.9b03641DOI Listing
December 2019

Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts.

Int J Biol Sci 2019 24;15(11):2340-2349. Epub 2019 Aug 24.

State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, China.

Induced pluripotent stem (iPS) cells provide a powerful platform for the study of development, regeneration, and disease. Although many stable iPS cell lines have been established for mammals, few attempts have been made to induce iPS cells in nonmammalian species. Because of technical advantages over other vertebrates on stem cells, induced pluripotent stem cells from fish could be of value for research. In this paper, stable iPS-like cell lines were generated from adult zebra fish fibroblasts by combining the doxycycline inducible lentiviral delivery system and chemical treatment. RT-PCR analysis, alkaline phosphatase staining, and immunofluorescence indicated that adult zebra fish fibroblasts were successfully reprogrammed into iPS-like cells (ziPSCs). The ziPSCs exhibited stable growth and manifested many features of fish embryonic stem cells with pluripotency in vitro and in vivo. Because of easy maintenance, the developed technology in this study for generating zebra fish iPS-like cells could be extended to investigating other genera of fish.
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http://dx.doi.org/10.7150/ijbs.34010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775306PMC
May 2020

The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection.

PLoS Pathog 2019 07 17;15(7):e1007917. Epub 2019 Jul 17.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.

It is important that bacterium can coordinately deliver several effectors into host cells to disturb the cellular progress during infection, however, the precise role of effectors in host cell cytosol remains to be resolved. In this study, we identified a new bacterial virulence effector from pathogenic Edwardsiella piscicida, which presents conserved crystal structure to thioredoxin family members and is defined as a thioredoxin-like protein (Trxlp). Unlike the classical bacterial thioredoxins, Trxlp can be translocated into host cells, mimicking endogenous thioredoxin to abrogate ASK1 homophilic interaction and phosphorylation, then suppressing the phosphorylation of downstream Erk1/2- and p38-MAPK signaling cascades. Moreover, Trxlp-mediated inhibition of ASK1-Erk/p38-MAPK axis promotes the pathogenesis of E. piscicida in zebrafish larvae infection model. Taken together, these data provide insights into the mechanism underlying the bacterial thioredoxin as a virulence effector in downmodulating the innate immune responses during E. piscicida infection.
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http://dx.doi.org/10.1371/journal.ppat.1007917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636751PMC
July 2019

Helicobacter pylori CagA promotes the malignant transformation of gastric mucosal epithelial cells through the dysregulation of the miR-155/KLF4 signaling pathway.

Mol Carcinog 2019 08 4;58(8):1427-1437. Epub 2019 Jun 4.

Department of physiopathology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, PR China.

The Helicobacter pylori (H. pylori) cytotoxin-associated gene A (CagA) and Krüppel-like transcription factor (KLF4) were both closely associated with the development and progression of gastric cancer (GC). However, the nature of the interactions between CagA and KLF4 in GC development has not been elucidated. Therefore, we focused on the CagA-mediated promotion of the malignant transformation of gastric epithelial cells. Herein, we first examined the expression of KLF4 in both human cancer and paracarcinoma tissues with or without H. pylori infection and found that KLF4 expression was significantly decreased in H. pylori-positive GC cells compared with the H. pylori-negative GC cells. Further functional studies revealed that the increased expression of CagA could suppress KLF4 expression and promote the malignant transformation of normal epithelial cells. Subsequently, we found that CagA could upregulate miR-155 and further restrict the expression of downstream KLF4. More importantly, the overexpression of miR-155 in GES-1 promoted epithelial-mesenchymal transition and eventually facilitated tumor growth in vivo. Overall, the identification of the CagA/miR-155/KLF4 signaling pathway provided a new insight into the development and treatment of GC.
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http://dx.doi.org/10.1002/mc.23025DOI Listing
August 2019

Differential diagnostic ability of 18F-FDG PET/CT radiomics features between renal cell carcinoma and renal lymphoma.

Q J Nucl Med Mol Imaging 2021 Mar 27;65(1):72-78. Epub 2019 May 27.

National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, China -

Background: The aim of this study is to determine the differential diagnostic value of texture parameters of PET/CT on renal cell carcinoma and renal lymphoma.

Methods: Twenty renal lymphoma and 18 renal cell carcinoma (RCC) patients were analyzed in this study. The pathological information and basic characteristics were extracted from the electronic medical record system of our hospital. We used LIFEx package to extract data from the radiomics images. Receiver operating characteristic analysis and binary logistic regression analysis was applied in determining the diagnostic accuracy of texture parameters as well as the synthetic parameter, of which the sensitivity and specificity was improved.

Results: There were 14 (two in Histogram, two in Grey Level Co-occurrence Matrix, five in Grey-Level Run Length Matrix, five in Grey-Level Zone Length Matrix) out of the texture parameters showing an area under the curve (AUC) >0.7 and P<0.05. Synthesized parameters of each section showed even higher differentiation ability, with AUC varying from 0.725 to 1.000.

Conclusions: Texture analysis of F-FDG PET/CT could effectively differentiate between RCCs and renal lymphomas.
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http://dx.doi.org/10.23736/S1824-4785.19.03137-6DOI Listing
March 2021
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