Publications by authors named "Wenting Li"

232 Publications

Sequential activation of heterogeneous macrophage phenotypes is essential for biomaterials-induced bone regeneration.

Biomaterials 2021 Jul 28;276:121038. Epub 2021 Jul 28.

Department of Orthopaedics & Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, 999077, China; Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, Department of Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518053, China; Cixi Center of Biomaterials Surface Engineering, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Ningbo, PR China. Electronic address:

Macrophage has been gradually recognized as a central regulator in tissue regeneration, and the study of how macrophage mediates biomaterials-induced bone regeneration through immunomodulatory pathway becomes popular. However, the current understanding on the roles of different macrophage phenotypes in regulating bone tissue regeneration remains controversial. In this study, we demonstrate that sequential infiltration of heterogeneous phenotypes of macrophages triggered by bio-metal ions effectively facilitates bone healing in bone defect. Indeed, M1 macrophages promote the recruitment and early commitment of osteogenic and angiogenic progenitors, while M2 macrophages and osteoclasts support the deposition and mineralization of the bone matrix, as well as the maturation of blood vessels. Moreover, we have identified a group of bone biomaterial-related multinucleated cells that behave similarly to M2 macrophages with wound-healing features rather than participate in the bone resorption cascade similarly to osteoclasts. Our study shows how sequential activation of macrophage-osteoclast lineage contribute to a highly orchestrated immune response in the bone tissue microenvironment around biomaterials to regulate the complex biological process of bone healing. Therefore, we believe that the temporal activation pattern of heterogeneous macrophage phenotypes should be considered when the next generation of biomaterials for bone regeneration is engineered.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121038DOI Listing
July 2021

The chicken pan-genome reveals gene content variation and a promoter region deletion in IGF2BP1 affecting body size.

Mol Biol Evol 2021 Jul 30. Epub 2021 Jul 30.

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.

Domestication and breeding have reshaped the genomic architecture of chicken, but the retention and loss of genomic elements during these evolutionary processes remain unclear. We present the first chicken pan-genome constructed using 664 individuals, which identified an additional ∼66.5 Mb sequences that are absent from the reference genome (GRCg6a). The constructed pan-genome encoded 20,491 predicated protein-coding genes, of which higher expression level are observed in conserved genes relative to dispensable genes. Presence/absence variation (PAV) analyses demonstrated that gene PAV in chicken was shaped by selection, genetic drift, and hybridization. PAV-based GWAS identified numerous candidate mutations related to growth, carcass composition, meat quality, or physiological traits. Among them, a deletion in the promoter region of IGF2BP1 affecting chicken body size is reported, which is supported by functional studies and extra samples. This is the first time to report the causal variant of chicken body size QTL located at chromosome 27 which was repeatedly reported. Therefore, the chicken pan-genome is a useful resource for biological discovery and breeding. It improves our understanding of chicken genome diversity and provides materials to unveil the evolution history of chicken domestication.
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http://dx.doi.org/10.1093/molbev/msab231DOI Listing
July 2021

Genome sequence resource of Xanthomonas citri pv. citri from formalin-fixed citrus leaf specimens showing canker lesions collected in 1982.

Plant Dis 2021 Jul 30. Epub 2021 Jul 30.

South China Agricltural University, Department of Plant Pathology, Guangzhou, Guangdong, China;

Xanthomonas citri pv. citri (Xcc) is the causative agent of citrus canker, one of the most devastating disease threatening global citrus production. Here, we present the genome sequence of Xcc strain GD82 from a formalin-fixed citrus leaf specimen showing canker lesions collected at 1982 in Guangdong Province, China. The GD82 genome comprised of 5,197,217 bp with G+C content of 64.8 %, along with four circular plasmids, pXAC33 (32,377 bp), pXAC64 (63,972 bp), pXAC47 (47,810 bp) and pGD82.1 (219,560 bp). This is the oldest Xcc genome from historical citrus canker specimens in China, which will enrich the current Xcc genome database and facilitate genomic evolution research of Xcc.
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http://dx.doi.org/10.1094/PDIS-05-21-1100-ADOI Listing
July 2021

Evaluation of Peroxidase in Herbal Medicines Based on an Electrochemical Sensor.

Front Chem 2021 23;9:709487. Epub 2021 Jun 23.

Department of Anorectal, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, China.

Peroxidases are species-specific. Differences in peroxidase can objectively reflect the genetics among species. The use of peroxidase to assist in species identification is relatively simple and effective. In this work, we proposed a graphene-modified electrode. This electrode can amplify the signal of electrocatalytic reduction of hydrogen peroxide. Since peroxidase can catalyze the reduction of hydrogen peroxide, this signal can be used as an indicator to demonstrate the content of peroxidase in different plant tissues. Twelve herbal medicines were selected for our study. The results show that this electrochemical-based detection technique was comparable to colorimetric method in terms of accuracy.
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http://dx.doi.org/10.3389/fchem.2021.709487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260690PMC
June 2021

pH-responsive black phosphorus quantum dots for tumor-targeted photodynamic therapy.

Photodiagnosis Photodyn Ther 2021 Jul 6;35:102429. Epub 2021 Jul 6.

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Collaborative Innovation Centre for Optoelectronic Science & Technology, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, China; College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Micro-Nano Photonic Information Technology, Guangdong Laboratory of Artificial Intelligence and Digital Economy (SZ), Shenzhen University, Shenzhen 518060, China. Electronic address:

Black phosphorus quantum dots(BPQDs) have shown a good application prospect in the field of tumor therapy due to their photoelectric effect and good biodegradability. Due to the active endocytosis and fast metabolic efficiency of tumor cells, BPQDs are easy to be absorbed by tumor cells. However, this does not guarantee that BPQDs will be completely targeted to tumor cells, and normal cells will also absorb BPQDs. Because the cell membrane is negatively charged, BPQDs are also negatively charged and are not easily absorbed by cells under the action of electrostatic repulsion. Surface pegylation is the most common modification method of black phosphorus at present. However, surface pegylation can reduce the uptake of BPQDs by tumor cells. Positive PEG is also easy to be recognized and swallowed by the reticuloendothelial system. The inherent instability and poor tumor targeting of BPQDs under physiological conditions limit further research and clinical application. For this purpose, we selected cationic polymer polyethylenimine (PEI) to modify BPQDs and then added RGD peptides targeting tumor cells. An outer layer of negatively charged PEG+DMMA makes the nanosystem more stable . In the acidic environment of the tumor, the PEG layer has a charge reversal, and the positively charged PEI and the RGD polypeptide BPQDs targeted by the tumor cells are released into the tumor cells. It provides a new method for efficiently and accurately transporting BPQDs, a novel photosensitive nanomaterial, into tumor cells for photodynamic therapy.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102429DOI Listing
July 2021

Effect of Dietary Tryptophan on Growth, Intestinal Microbiota, and Intestinal Gene Expression in an Improved Triploid Crucian Carp.

Front Nutr 2021 17;8:676035. Epub 2021 Jun 17.

State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, China.

Tryptophan (Trp) has received increasing attention in the maintenance of intestinal function. In this study, improved triploid crucian carp (ITCC) fed diets containing 6.35 g kg Trp had higher average daily gain (ADG) and improved villus height (VH) and crypt depth (CD) in the intestine compared to the control group. To elucidate the potential mechanisms, we used RNA sequencing (RNA-seq) to investigate changes in the intestinal transcriptome and 16S rRNA gene sequencing to measure the intestinal microbiota in response to 6.35 g kg Trp feeding in ITCC. Dietary Trp altered intestinal gene expression involved in nutrient transport and metabolism. Differentially expressed transcripts (DETs) were highly enriched in key pathways containing protein digestion and absorption and the AMPK signaling pathway. 16S rRNA sequencing showed that 6.35 g kg Trp significantly increased the abundance of the genus , and the Firmicutes/Bacteroidetes ratio at the phylum level ( < 0.05). In addition, bacterial richness indices (Simpson index) significantly increased ( < 0.05) community evenness in response to 6.35 g kg Trp. In conclusion, appropriate dietary Trp improves the growth performance, and influences the intestinal flora of ITCC. This study might be helpful to guide the supply of dietary exogenous Trp in ITCC breeding.
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http://dx.doi.org/10.3389/fnut.2021.676035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247481PMC
June 2021

Characterization of nanoparticles combining polyamine detection with photodynamic therapy.

Commun Biol 2021 Jul 1;4(1):803. Epub 2021 Jul 1.

Department of Physics, University of Texas at Arlington, Arlington, TX, USA.

Polyamine detection and depletion have been extensively investigated for cancer prevention and treatment. However, the therapeutic efficacy is far from satisfactory, mainly due to a polyamine compensation mechanism from the systemic circulation in the tumor environment. Herein, we explore a new solution for improving polyamine detection as well as a possible consumption therapy based on a new photosensitizer that can efficiently consume polyamines via an irreversible chemical reaction. The new photosensitizer is pyrrolopyrroleaza-BODIPY pyridinium salt (PPAB-PyS) nanoparticles that can react with the over-expressed polyamine in cancer cells and produce two photosensitizers with enhanced phototoxicity on cancer destruction. Meanwhile, PPAB-PyS nanoparticles provide a simultaneous ratiometric fluorescence imaging of intracellular polyamine. This combination polyamine consumption with a chemical reaction provides a new modality to enable polyamine detection along with photodynamic therapy as well as a putative depletion of polyamines for cancer treatment and prevention.
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http://dx.doi.org/10.1038/s42003-021-02317-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249666PMC
July 2021

Significance of miR-141 and miR-340 in cervical squamous cell carcinoma.

Open Med (Wars) 2021 16;16(1):864-872. Epub 2021 Jun 16.

Department of Pathology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang, China.

Background: We investigated the expression and clinical significance of miR-141 and miR-340 in cervical squamous cell carcinoma (CSCC).

Methods: Expression of miR-141 and miR-340 in CSCC, high-grade squamous intraepithelial lesion (HSIL), and normal cervical squamous epithelium were detected by qRT-PCR. PTEN was assessed by immunohistochemistry. Their relationship with clinicopathological features was analyzed.

Results: The changes of miR-141 and miR-340 were different in CSCC, HSIL, and normal squamous epithelium ( = 0.030). miR-141 expression was statistically significant in gross type, differentiation, uterine corpus invasion, nerve invasion, vagina invasion, and FIGO stage in CSCC ( < 0.05). miR-340 expression was related to tumor size, differentiation, nerve invasion, lymph node metastasis, and FIGO stage in CSCC ( < 0.05). miR-141 and miR-340 expressions were statistically significant in different ages ( < 0.05) in HSIL. The AUC of miR-141 in CSCC diagnosis and that of miR-340 in HSIL diagnosis were 0.893 and 0.764, respectively. The sensitivity and the specificity of miR-141 for diagnosis of CSCC were 95.0% and 60.8%, respectively, while those of miR-340 for diagnosis of HSIL were 90.0 and 48.6%, respectively. miR-141 and miR-340 expressions are associated with PTEN expression ( = 0.002 and < 0.001).

Conclusion: miR-141 and miR-340 may be associated with their target gene PTEN and involved in the carcinogenesis of cervical squamous epithelium.
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http://dx.doi.org/10.1515/med-2021-0281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209411PMC
June 2021

Effect of exogenous glucoamylase inclusion on in vitro fermentation and growth performance of feedlot steers fed a dry-rolled corn-based diet.

Transl Anim Sci 2021 Apr 10;5(2):txab082. Epub 2021 May 10.

Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.

The objective was to evaluate the effect of adding an exogenous glucoamylase (GA) enzyme from the fungus on in vitro fermentation, growth performance, and carcass characteristics of feedlot steers fed a dry-rolled corn (DRC)-based diet. Experiment 1 evaluated three levels of added enzyme (0, 0.24, and 0.72 GA enzyme units) and two corn particle sizes (CPS; 2 and 4 mm) in a factorial arrangement using a 7 h in vitro batch culture fermentation. Addition of GA increased ( < 0.01) in vitro dry matter disappearance by 13% and decreased final pH ( < 0.01). Molar proportion of propionate increased with GA inclusion ( < 0.01). A smaller CPS increased ( < 0.01) in vitro dry matter disappearance and total volatile fatty acid and decreased final pH ( < 0.01). A smaller CPS also decreased ( < 0.01) the molar proportion of acetate and increased ( < 0.01) the molar proportion of butyrate. In experiment 2, Angus × Simmental steers ( = 105; initial body weight [BW] = 329 ± 38 kg) were used to evaluate the inclusion of an exogenous GA on growth performance and carcass characteristics. Steers were fed a basal diet consisting of 60% DRC, 17.5% modified distillers grains with solubles, 12.5% corn silage, and 10% dry supplement on a dry matter basis for 136 d. Steers were blocked by weight and allotted to pens. Pens were randomly assigned to one of three treatments (5 pens/treatment): diet with no GA (CON), low inclusion of GA (122 enzyme units/kg dry matter [DM]; LGA), or high inclusion of GA (183 enzyme units/kg DM; HGA). Inclusion of GA did not affect ( ≥ 0.23) final BW, dry matter intake (DMI), or average daily gain (ADG) for the 136-d feeding period. Feed conversion was affected ( = 0.02) by treatment with steers fed HGA having ~8% greater G:F compared with LGA and CON. Treatment did not affect ( = 0.32) fecal starch. Inclusion of GA did not affect ( ≥ 0.19) carcass traits including hot carcass weight, 12th rib fat thickness, yield grade, longissimus muscle area, or marbling score. Overall, results suggest inclusion of exogenous GA enzyme increased in vitro dry matter disappearance in batch culture and improved feed conversion in steers fed 183 enzyme units/kg DM during the finishing phase.
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http://dx.doi.org/10.1093/tas/txab082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212169PMC
April 2021

DNAzyme-Catalyzed Cellular Oxidative Stress Amplification for Pro-protein Activation in Living Cells.

Chembiochem 2021 Jun 21. Epub 2021 Jun 21.

Beijing National Laboratory for Molecular Science, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun North First Street 2, 100190, Beijing, P. R. China.

The conditional control of protein function in response to the physiological change of cells is of great interest for studying protein function in biological settings and developing protein therapeutics. We report herein that catalase (CAT) DNAzyme can potentiate the generation of reactive oxygen species (ROS) in living cells by knocking down catalase expression, which could further activate a reactive oxygen species (ROS)-responsive pro-protein, RNase A-NBC, in situ. Using an optimized lipid nanoparticle delivery system to simultaneously introduce CAT DNAzyme and RNase A-NBC into cells, we show that the pro-protein, RNase A-NBC, could be activated in a significantly enhanced manner to prohibit tumor cell growth in different types of cancer cells. We believe the methodology of regulating pro-protein activity using DNAzyme biocatalysis to differentiate intracellular environment could further be extended to other functional proteins, and even fundamental investigations in living systems to develop pro-protein therapeutics.
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http://dx.doi.org/10.1002/cbic.202100225DOI Listing
June 2021

C3 ester side chain plays a pivotal role in the antitumor activity of Maytansinoids.

Biochem Biophys Res Commun 2021 Aug 15;566:197-203. Epub 2021 Jun 15.

State Key Laboratory of Biotherapy and Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, PR China. Electronic address:

Maytansinoids, the chemical derivatives of Maytansine, are commonly used as potent cytotoxic payloads in antibody-drug conjugates (ADC). Structure-activity-relationship studies had identified the C3 ester side chain as a critical element for antitumor activity of maytansinoids. The maytansinoids bearing the methyl group at C3 position with D configuration were about 100 to 400-fold less cytotoxic than their corresponding L-epimers toward various cell lines. The detailed mechanism of how chirality affects the anticancer activity remains elusive. In this study, we determined the high-resolution crystal structure of tubulin in complex with maytansinol, L-DM1-SMe and D-DM1-SMe. And we found the carbonyl oxygen atom of the ester moiety and the tail thiomethyl group at C3 side chain of L-DM1-SMe form strong intramolecular interaction with the hydroxyl at position 9 and the benzene ring, respectively, fixing the bioactive conformation and enhancing the binding affinity. Additionally, ligand-based and structure-based virtually screening methods were used to screen the commercially macrocyclic compounds library, and 15 macrocyclic structures were picketed out as putatively new maytansine-site inhibitors. Our study provides a possible strategy for the rational discovery of next-generation maytansine site inhibitors.
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http://dx.doi.org/10.1016/j.bbrc.2021.05.071DOI Listing
August 2021

Effects of exogenous α-amylases, glucoamylases, and proteases on ruminal in vitro dry matter and starch digestibility, gas production, and volatile fatty acids of mature dent corn grain.

Transl Anim Sci 2021 Jan 28;5(1):txaa222. Epub 2020 Nov 28.

Department of Animal Sciences, University of Florida, Gainesville, FL.

Two separate experiments were carried out to evaluate the effects of incremental doses of 10 exogenous endo-acting α-amylase and exo-acting glucoamylase; 1LAT (bacterial α-amylase), 2AK, 3AC, 4Cs4, 5Trga, 6Afuga, 7Fvga, and 10Tg (fungal α-amylases, glucoamylases, and α-glucosidase), 8Star and 9Syn (fungal amylase-mixtures; experiment 1) and three exogenous proteases; 11P14L, 12P7L, and 13P30L (bacterial proteases; experiment 2) on in vitro dry matter digestibility (IVDMD) and in vitro starch digestibility (IVSD) of mature dent corn grain using a batch culture system. Incremental doses of the exogenous enzymes (0, 0.25, 0.50, 0.75, and 1.00 mg/g of dried substrate) were applied directly to the substrate (0.5 g of ground corn, 4 mm) in sextuplicate (experiment 1) or quadruplicate (experiment 2) within F57 filter bags, which were incubated at 39 °C in buffered rumen fluid for 7 h. Rumen fluid was collected 2-3 h after the morning feeding from three lactating dairy cows and pooled. Cows were consuming a midlactation total mixed ration (TMR; 1.60 Mcal/kg DM and 15.4%; net energy of lactation and crude protein, respectively). Three independent runs were carried out for each experiment. Data were analyzed as a randomized complete block design using run as the blocking factor. Dose was used as a fixed factor while run was considered a random factor. Linear, quadratic, and cubic orthogonal contrasts were also tested. In experiment 1, enzymes 2AK, 3AC, and 10Tg did not increase ( > 0.10) IVDMD and IVSD, whereas 0.25 mg of enzymes 1LAT, 5Trga, and 8Star increased ( < 0.01) IVDMD by 23%, 47%, and 62% and IVSD by 35%, 41%, and 58%, respectively, compared with the control. Enzymes 4Cs4, 6Afuga, 7Fvga, and 9Syn linearly increased IVDMD and IVSD ( < 0.01). Greatest increases in IVDMD (82.9%) and IVSD (85.9%) resulted with 1 mg of 6Afuga compared to control. In experiment 2, the lowest dose of exogenous proteases 11P14L and 12P7L increased ( < 0.01) IVDMD by 98% and 87% and IVSD by 57% and 64%, respectively, whereas the highest dose of 13P30L increased ( = 0.02) IVDMD by 44.8% and IVSD by 30%, relative to the control. In conclusion, IVSD and IVDMD were increased by one α-amylase, certain glucoamylases, and all proteases tested, with the glucoamylase 6Afuga in experiment 1 and the neutral protease 12P7L in experiment 2, increasing IVDMD and IVSD to the greater extents. Future in vivo studies are required to validate these findings before these enzyme additives can be recommended for improving the digestibility of mature dent corn grain.
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http://dx.doi.org/10.1093/tas/txaa222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205108PMC
January 2021

Synergistic effect of defects and porous structure in CoCCHH-CoSe heterogeneous-tube @PEDOT:PSS foam towards elastic supercapacitor with enhanced pseudocapacitances.

J Colloid Interface Sci 2021 May 30;602:251-260. Epub 2021 May 30.

School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, PR China.

It is still challenging to construct stable 3D energy storage materials at the nanoscale by precise pore structure control and reasonable surface modification. Herein, a novel interwoven porous Co(CO)Cl(OH) (CoCCHH)-CoSe heterogeneous-tube @PEDOT:PSS 3D foam with abundant active sites is presented as supercapacitor electrodes. The electrochemical results indicated that the pore structure provides ample space for redox reaction, and increases the number of ion transport channels. Besides, rational surface modification brings about sufficient active sites for redox reaction. The stable, porous PEDOT:PSS foam with a 3D elastic frame exhibited excellent electrical conductivity. Thus, the [email protected]:PSS foam possessed excellent specific capacitance and energy density, due to the synergistic effect of the unique 3D structure and surface defects. The home-made supercapacitor with [email protected]:PSS foam as cathode materials showed high specific capacitance (440.6F g at 1 A g) and excellent energy density (137.7 Wh kg). This work provides a valuable strategy to develop potential materials for electrochemical energy storage.
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http://dx.doi.org/10.1016/j.jcis.2021.05.160DOI Listing
May 2021

Identification of genes related to stress affecting thymus immune function in a chicken stress model using transcriptome analysis.

Res Vet Sci 2021 Sep 7;138:90-99. Epub 2021 Jun 7.

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China. Electronic address:

With the rapid development of the poultry breeding industry and highly intensive production management, the losses caused by stress responses are becoming increasingly serious. To screen candidate genes related to chicken stress and provide a basis for future research on the molecular mechanisms governing the effects of stress on chicken immune function, we successfully constructed a chicken stress model by exogenously introducing corticosterone (CORT). RNA-seq technology was used to identify and analyze the mRNA and enrichment pathways of the thymus in the stress model group and the control group. The results showed that there were 101 significantly differentially expressed genes (SDEGs) (Padj < 0.05, |log2fold changes| ≥ 1 and FPKM >1), of which 44 were upregulated genes, while 57 were downregulated genes. Gene Ontology (GO) enrichment analysis found that the terms related to immunity or stress mainly included antigen processing and presentation, positive regulation of T cell-mediated immunity, and immune effector process. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the main pathways related to immunity or stress were the PPAR signaling pathway, NOD-like receptor signaling pathway, and intestinal immune network for IgA production. Among the SDEGs, XCL1, HSPA8, DMB1 and BAG3 are strongly related to immunity or stress and may be important genes involved in regulating stress affecting the immune function of chickens. The above results provide a theoretical reference for subsequent research on the molecular regulatory mechanisms by which stress affects the immune function of poultry.
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http://dx.doi.org/10.1016/j.rvsc.2021.06.006DOI Listing
September 2021

Gold nanoparticles-mediated ratiometric fluorescence aptasensor for ultra-sensitive detection of Abscisic Acid.

Biosens Bioelectron 2021 Oct 7;190:113311. Epub 2021 May 7.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang, 310058, China. Electronic address:

Herein, a novel ratiometric aptasensor based on carbon quantum [email protected] zinc salt ([email protected]) and aptamer-functionalized gold nanoparticles (Apt-AuNPs) was developed for highly sensitive detection of ABA by fluorescence spectrometry. The [email protected] nanomaterials displayed dual-emission properties at 490 nm and 657 nm with excitation at 420 nm were synthesized for the first time. ZIF-8 not only served as an anchor point for CQDs but also acted as a modulator to regulate fluorescence signals of CQDs. Interestingly, introduction of ZIF-8 changed the quenching properties of the AuNPs on CQDs. The AuNPs quenched the fluorescence of [email protected] at 490 nm but not at the second peak of 657 nm. Few studies have been reported on the ineffectiveness of AuNPs in fluorescence quenching as far as we know. In this study, we found that incorporation of ABA triggered the aggregation of AuNPs due to the specific ABA-aptamer recognition and this changed the fluorescence intensity of the ratiometric probe ([email protected]@Apt-AuNPs). The proposed probe increased the sensitivity and selectivity of determining ABA levels in rice seeds in the range of 0.100-150 ng/mL with an LOD of 30.0 ng/L. Importantly, the method proposed here offers a new unique strategy for the construction of ratiometric probes and ultra-sensitive measurement of biomolecules.
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http://dx.doi.org/10.1016/j.bios.2021.113311DOI Listing
October 2021

The X-ray structure of tubulysin analogue TGL in complex with tubulin and three possible routes for the development of next-generation tubulysin analogues.

Biochem Biophys Res Commun 2021 Aug 2;565:29-35. Epub 2021 Jun 2.

State Key Laboratory of Biotherapy and Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China; Department of Geriatrics and National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China. Electronic address:

Microtubule-targeting agents (MTAs) are the most commonly used anti-cancer drugs. At least fourteen microtubule inhibitors and ten antibody drug conjugates (ADCs) linking MTAs are approved by FDA for clinical use in cancer therapy. In current research, we determined the crystal structure of tubulysin analogue TGL in complex with tubulin at a high resolution (2.65 Å). In addition, we summarized all of the previously published high-resolution crystal structures of ligands in the vinca site to provide structural insights for the rational design of the new vinca-site ligands. Moreover, based on the aligned results of the vinca site ligands, we provided three possible routes for designing new tubulysin analogues, namely macrocyclization between the N-14 side chain and the N-9 side chain, the hybird of tubulysin M and phomopsin A, and growing new aryl group at C-21. These designed structures will inspire the development of new MTAs or payloads in cancer therapy.
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http://dx.doi.org/10.1016/j.bbrc.2021.05.086DOI Listing
August 2021

Photodynamic immunotherapy of cancers based on nanotechnology: recent advances and future challenges.

J Nanobiotechnology 2021 May 29;19(1):160. Epub 2021 May 29.

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Collaborative Innovation Centre for Optoelectronic Science & Technology, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen, 518060, China.

Photodynamic therapy (PDT) is a non-invasive or minimally-invasive treatment which applies photosensitizers (PSs) to create reactive oxygen species (ROS) exposed to light trigger to destroy cancer cells. PDT can activate host anti-tumor immune responses but not powerful enough to kill metastatic tumors. Because of its carrier advantage, imaging, and therapeutic function together with enhanced permeability and retention (EPR) effect, nano-materials have already been used in photo-immunotherapy. Herein, photodynamic immunotherapy (PDIT) based on nanotechnology seems to be a hopeful new form of cancer therapy. In this article, we firstly summarize the recent development in photodynamic immunotherapy based on nanotechnology.
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http://dx.doi.org/10.1186/s12951-021-00903-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164771PMC
May 2021

Digital PCR assay for the effective detection of COVID-19 patients with SARS-CoV-2 low viral load.

J Virol Methods 2021 09 26;295:114185. Epub 2021 May 26.

First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China. Electronic address:

Objective: Viral nucleic acid detection by real-time reverse transcription polymerase chain reaction (qPCR) is the current standard method for diagnosis of SARS-CoV-2 infection. However, due to low viral load in some COVID-19 patients, false negative results from this method have been repeatedly reported.

Method: In this study, we compared the sensitivity and specificity of digital PCR (dPCR) in simulated samples and clinical samples with qPCR assay through a series of vigorous tests.

Results: The results showed that dPCR was more sensitive than qPCR especially for samples with low viral load (≤3 copies). In addition, dPCR had similar specificity as qPCR and could effectively distinguish other human coronaviruses and influenza virus from SARS-CoV-2. More importantly, dPCR was more sensitive than qPCR in detecting the virus in the "negative" samples from recurrent COVID-19 patients.

Conclusions: In summary, dPCR could serve as a powerful complement to the current qPCR method for SARS-CoV-2 detection, especially for the samples with extremely low viral load, such as recurrent COVID-19 patients.
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http://dx.doi.org/10.1016/j.jviromet.2021.114185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149472PMC
September 2021

SIRT6 through PI3K/Akt/mTOR signaling pathway to enhance radiosensitivity of non-Small cell lung cancer and inhibit tumor progression.

IUBMB Life 2021 May 25. Epub 2021 May 25.

Department of Oncology, People's Hospital of Chongqing Banan District, Chongqing, China.

To explore the effect and mechanism of SIRT6 on radiosensitivity and tumor progression of non-small cell lung cancer (NSCLC). qRT-PCR was performed to detect the expressions of SIRT6 in tumor tissues, adjacent normal tissues and NSCLC cell lines of patients with advanced NSCLC before and after radiotherapy. After overexpression or interference with SIRT6 expression in NSCLC cells, the cells were routinely cultured or transfected for 48 h followed by 4 Gy radiation for 24 h. Then, check the cell proliferation, migration, apoptosis and cell cycle by MTT, wound healing assay and flow cytometry, while detect the expression of PI3K/Akt/mTOR signaling pathway-related proteins by Western blot. In addition, the effect of SIRT6 expression on NSCLC tumor growth was analyzed by xenograft tumor assay. SIRT6 showed a low expression in NSCLC tumor tissues and cell lines, while SIRT6 was significantly increased in NSCLC tissues after radiation treatment. Overexpression of SIRT6 in A549 and NCI-H23 cells inhibited cell proliferation viability, migration ability and promoted apoptosis. By comparison, after radiation treatment, NSCLC cells with high SIRT6 expression had lower ability of proliferation and migration and higher apoptosis rate. Overexpression of SIRT6 evidently down-regulated the activity of PI3K/Akt/mTOR signaling pathway in NSCLC cells before and after radiation. In addition, H2009 cells exhibited opposite cellular functions after interference with SIRT6 expression. In vivo experiments showed that overexpression of SIRT6 promoted the inhibitory effect of radiation on the growth of NSCLC xenograft tumors in nude mice. SIRT6 can promote the radiosensitivity of NSCLC and inhibit the development of tumor by down-regulating the activity of PI3K/Akt/mTOR signaling pathway.
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http://dx.doi.org/10.1002/iub.2511DOI Listing
May 2021

Diagnostic yield of Xpert MTB/RIF on contrast-enhanced ultrasound-guided pleural biopsy specimens for pleural tuberculosis.

Int J Infect Dis 2021 Jul 14;108:89-95. Epub 2021 May 14.

Department of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai Clinic and Research Center of Tuberculosis, Shanghai Key Laboratory of Tuberculosis, Shanghai, China. Electronic address:

Background: A 3-year prospective study was conducted to evaluate the efficacy of Xpert MTB/RIF (Xpert) in the diagnosis of pleural tuberculosis (pTB) on contrast -enhanced ultrasound (CEUS)-guided pleural biopsy specimens.

Method: Patients suspected with pTB were prospectively enrolled to receive CEUS-guided biopsy. Specimens (pleural tissue and fluid) were submitted for Xpert and other routine examinations. Surgical thoracoscopy was performed on undiagnosed cases.

Result: A total of 316 patients were enrolled, including 280 cases of pTB (definite 195, possible 85) and 36 cases of non-pTB. The sensitivity of Xpert was 69.64% (195/280) in biopsy specimens, which was significantly higher than that in pleural effusion specimens (p < 0.01). In 195 definite cases, the highest sensitivity of 100% (195/195) and NPV of 29.75% (36/121) were achieved by Xpert on biopsy specimens. Xpert-positive results were obtained in 149 culture-negative cases and 90 histopathological MTB PCR-negative cases. The incidence of necrosis by CEUS in Xpert-positive pTB was significantly higher than that in Xpert-negative pTB (χ = 72.41; p < 0.01). No serious complications occurred.

Conclusion: Xpert achieved highly diagnostic sensitivity in pTB through CEUS-guided biopsy sampling, especially on necrotic lesions, which was proven to be efficient, minimally invasive and safe.
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http://dx.doi.org/10.1016/j.ijid.2021.05.023DOI Listing
July 2021

A rapid density method for taxi passengers hot spot recognition and visualization based on DBSCAN.

Sci Rep 2021 May 3;11(1):9420. Epub 2021 May 3.

Faculty of Computer and Software Engineering, Huaiyin Institute of Technology, Huaian, 223003, People's Republic of China.

With the development of city size and vehicle interconnection, visual analysis technology is playing a very important role in the course of city calculation and city perception. A Reasonable visual model can effectively present the feature of city. In order to solve the problem of traditional density algorithm that cluster the large scale data slowly and cannot find cluster centers to adapt taxi track data. The DBSCAN (density-based spatial clustering of applications with noise plus) algorithm that can split data and extract maximum density clusters under the large scale data was proposed in the paper. The passenger points should be cleaned from the original point of the passenger trajectory data firstly, and then the massive passenger points are sliced and clustered cyclically. In the clustering process, the cluster centers can be extracted based on maximum density, and finally the clustering results are visualized according to the results. The experimental results show that compared with other popular methods, the proposed method has significant advantages in clustering speed, precision and visualization for large-scale city passenger hotspots. Moreover, it provides important decisions for further urban planning and promotes the traffic efficiency.
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http://dx.doi.org/10.1038/s41598-021-88822-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093244PMC
May 2021

Analysis of individualized antiplatelet therapy for patients of acute coronary syndrome after percutaneous coronary intervention under the guidance of platelet function: A one-center retrospective cohort study.

Medicine (Baltimore) 2021 Apr;100(16):e25601

Department of Cardiology, Tianjin Union Medical Center.

Abstract: There is controversy in clinical application of antiplatelet drugs by monitoring platelet function. Therefore, we explored whether early and dynamic medication could bring better clinical outcomes for patients under the guidance of platelet function tests (PFT).In this retrospective cohort study, we analyzed the prognostic events of 1550 patients with acute coronary syndrome (ACS) at Tianjin People's Hospital in China. They received dual antiplatelet therapy (DAPT) after percutaneous coronary intervention (PCI) from January 2017 to December 2018. The primary endpoint was based on the Bleeding Academic Research Consortium (BARC) 3 or 5 major bleeding. Secondary endpoints included MACCE (all-cause death, nonfatal myocardial infarction, stroke, stent thrombosis, and unplanned target vessel reconstruction) and BARC 1 to 2 minor bleeding. The endpoint events within 1 year after PCI were recorded. Patients were divided into a guided group and a control group according to the drug adjustment by PFT results. After the propensity scores matched, the end points of 2 groups were compared, and subgroup analysis was performed on major bleeding events.After propensity score matching, there were 511 cases in the guided group and the control group, respectively. The primary endpoint events occurred in 10 patients (1.96%) in the guided group and 23 patients (4.5%) in the control group (HR: 0.45; 95% CI, 0.21-0.95; P = .037). After the guided group adjusted drug doses, the risk of major bleeding was lower than standard DAPT of the control group. Although some patients in the guided group reduced doses earlier, the incidence of MACCE events did not increase in the guided group compared with the control group (4.89% vs 6.07%; P = .41). There was no statistical difference in BARC 1 to 2 minor bleeding (P = .22). Subgroup analysis showed that PFT was more effective in patients with diabetes and multivessel disease.Early observation of dynamic PFT in ACS patients after PCI can guide individualized antiplatelet therapy to reduce the risk of major bleeding without increasing the risk of ischemia.
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http://dx.doi.org/10.1097/MD.0000000000025601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078371PMC
April 2021

Rapid detection of cadmium ions in meat by a multi-walled carbon nanotubes enhanced metal-organic framework modified electrochemical sensor.

Food Chem 2021 Apr 9;357:129762. Epub 2021 Apr 9.

Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China; China-UK Joint Laboratory for Nondestructive Detection of Agro-products, Jiangsu University, Zhenjiang, Jiangsu 212013, China.

This work presents an electrochemical device based on a composite modification of amine functionalized Zr(IV) metal-organic framework (UiO-66-NH) and multi-walled carbon nanotubes (MWCNTs) for voltammetry determination of cadmium ions (Cd). The [email protected] composites were prepared by one-pot hydrothermal reaction. The prepared sensor performs excellent performance, which was attributed to the synergism between UiO-66-NH with a special octahedral structure and enlarged surface area and MWCNTs with outstanding conductivity. Under optimal experiment condition, the fabricated sensor showed good linear relationship from 0.5 to 170 μg/L, with a detection limit of 0.2 μg/L. Finally, the sensor was successfully applied to detect Cd in meat samples (N = 21) with relative standard deviation (RSD) lower than 4.5% and recovery of 95.1-107.5%, and the results were compared with certified method, there was no statistical significance difference between the developed sensor and certified method at a 95% confidence level.
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http://dx.doi.org/10.1016/j.foodchem.2021.129762DOI Listing
April 2021

In-Vitro and In-Silico Assessment of Per- and Polyfluoroalkyl Substances (PFAS) in Aqueous Film-Forming Foam (AFFF) Binding to Human Serum Albumin.

Toxics 2021 Mar 17;9(3). Epub 2021 Mar 17.

Department of Civil and Environmental Engineering, University of California Davis, Davis, CA 95616, USA.

Drinking water contaminated by fluorosurfactant-based aqueous film-forming foams (AFFF) is a source of human exposure to poly- and perfluoroalkyl substances (PFAS). However, assessment of bioaccumulation potentials of diverse PFAS in commercial products such as AFFF have been insufficient and challenging, especially due to a lack of analytical standards. Here we explore the value of suspect screening, equilibrium dialysis, and molecular-docking simulations to identify potentially bioaccumulative PFAS. We exposed human serum albumin (HSA) protein to dilutions of a legacy AFFF produced by 3M in 1999 using equilibrium dialysis and screened in-vitro protein-binding affinities using high-resolution mass spectrometry (HRMS). Through suspect screening, we identified 32 PFAS and 18 hydrocarbon surfactants in the AFFF that bound to HSA. Quantification of noncovalent association constants for 26 PFAS standards confirmed that many PFAS, including the short-chain perfluoropropane sulfonic acid (log K= 4.1 ± 0.2 M), exhibit strong binding affinities with HSA. At least five PFAS in AFFF (including three PFAS with less than five perfluorocarbons) remained bound to the precipitated HSA pellet after extensive solvent washing-an indication of high PFAS binding potential. Three PFAS (PFBS, PFOS, and PFOA) were confirmed in the protein pellet with analytical standards and quantified after acid digestion-this sample fraction accounted for 5 to 20% of each compound mass in the sample. We calculated pseudo-bioconcentration factors (BCF) for PFAS that suspect screening flagged as noncovalently bound or potentially covalently bound. Most PFAS exhibiting high BCF, especially those with seven perfluorocarbons, contained a carboxylic acid or a sulfonic acid. Finally, we used molecular docking to simulate HSA binding affinities for 62 ligands (26 PFAS targets, 18 PFAS qualified in AFFF, and 18 hydrocarbon surfactants qualified in AFFF). We found that molecular docking can effectively separate HSA-binding and -nonbinding compounds in AFFF. In-vitro and in-silico approaches described in this study provide replicable, high-throughput workflows for assessing bioaccumulation potentials of diverse PFAS in commercial products.
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http://dx.doi.org/10.3390/toxics9030063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002870PMC
March 2021

Co-culture of fungi-microalgae consortium for wastewater treatment: A review.

Bioresour Technol 2021 Jun 17;330:125008. Epub 2021 Mar 17.

School of Land Resources and Environment, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address:

The treatment of wastewater by microalgae has been studied and proved to be effective through previous studies. Due to the small size of microalgae, how to efficiently harvest microalgae from wastewater is a crucial factor restricting the development of algal technologies. Fungi-assisted microalgae bio-flocculation for microalgae harvesting and wastewater treatment simultaneously, which was overlooked previously, has attracted increasing attention in the recent decade due to its low cost and high efficiency. This review found that fungal hyphae and microalgae can stick together due to electrostatic neutralization, surface protein interaction, and exopolysaccharide adhesion in the co-culture process, realizing co-pelletization of microalgae and fungi, which is conducive to microalgae harvesting. Besides, the combination of fungi and microalgae has a complementary effect on pollutant removal from wastewaters. The co-culture of fungi-microalgae has excellent development prospects with both environmental and economic benefits, and it is expected to be applied on an industrial scale.
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http://dx.doi.org/10.1016/j.biortech.2021.125008DOI Listing
June 2021

Direct interactions between cationic liposomes and bacterial cells ameliorate the systemic treatment of invasive multidrug-resistant Staphylococcus aureus infections.

Nanomedicine 2021 Jun 23;34:102382. Epub 2021 Mar 23.

School of Pharmacy, Shandong New Drug Loading & Release Technology and Preparation Engineering Laboratory, Binzhou Medical University, Yantai, PR China. Electronic address:

Invasive infections caused by antibiotic-resistant Staphylococcus aureus have posed a great threat to human health. To tackle this problem, a cationic liposomal Curcumin (C-LS/Cur) was developed and its effect against antibiotic-resistant S. aureus was investigated in this study. As expected, C-LS/Cur exhibited greater bactericidal capacity compared with its counterparts, probably because the negatively charged S. aureus favors electrostatic interactions rather than intercalation with cationic liposomal vesicles at the beginning of endocytic process, thereby effectively delivering Cur to its targets. We confirmed this hypothesis by monitoring zeta potential variation, collecting visual evidences through CLSM, FCM and TEM, and determining binding kinetics by BLI. Moreover, an excellent therapeutic efficacy of C-LS/Cur against invasive murine infection was also observed, which was due to the enhanced accumulation and retention in the targets. Therefore, cationic liposomes have great potential for the clinical application in the treatment of invasive antibiotic-resistant S. aureus infections.
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http://dx.doi.org/10.1016/j.nano.2021.102382DOI Listing
June 2021

Identification and expression analysis of MicroRNAs in chicken spleen in a corticosterone-induced stress model.

Res Vet Sci 2021 May 1;136:287-296. Epub 2021 Mar 1.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China. Electronic address:

For investigating the effects of stress on the immune response of chickens, we established a corticosterone (CORT)-induced stress model by exogenous intake of CORT. Control group was fed with a basal diet and the stress model group was fed with a 30 mg/Kg CORT-treated diet in ad libitum conditions for 7 days. Then, we used RNA-seq technology to identify the expression pattern of miRNAs, target genes, and relevant pathways in chicken spleen. Results showed that 71 differentially expressed miRNAs (DEMs) were determined, 9 of which were significantly differentially expressed miRNAs (SDEMs), and 241 target genes of DEMs were predicted. GO annotation and KEGG pathway analysis were carried out to understand the role of the DEMs. Out of 287 significantly enriched GO terms, 37 were stress- or immune-related, such as response to light stimulus, detection of oxidative stress, and immune response in mucosal-associated lymphoid tissue. Out of 85 KEGG pathways, 8 were related to stress or immunity, such as cytokine-cytokine receptor interaction, JAK-STAT signaling pathway, and RLR signaling pathway. We then constructed the interaction networks between target genes from immune-related pathways and their DEMs. The analysis results suggested that some DEMs (gga-miR-17 family, gga-miR-15/16 family, gga-miR-2954 and gga-miR-34b-5p) and target genes (SIKE1, CX3CL1, IL11Ra, PIGR, and CDKN1A) were core miRNAs and genes. This study revealed the dynamic miRNA transcriptome, target genes and related pathways in chicken spleen under CORT-induced stress model, which provided a basis for studying the molecular mechanism of stress affecting immune function.
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http://dx.doi.org/10.1016/j.rvsc.2021.02.023DOI Listing
May 2021

Analysis of miRNA and mRNA reveals core interaction networks and pathways of dexamethasone-induced immunosuppression in chicken bursa of Fabricius.

Mol Immunol 2021 06 10;134:34-47. Epub 2021 Mar 10.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China. Electronic address:

Stress-induced immunosuppression is a serious problem affecting the production value of poultry, but its specific molecular mechanism has not yet been elucidated. We selected 7-day-old Gushi cocks as test animals and successfully established a stress-induced immunosuppression model by injecting 2.0 mg/kg (body weight) dexamethasone (Dex). We then constructed six cDNA libraries and two small RNA libraries of Bursa of Fabricius from the control group and the Dex group. RNA-seq results revealed 21,028 transcripts including 3920 novel transcripts; 500 miRNAs including 68 novel miRNAs were identified. Correlation analysis of miRNA, target genes and mRNA results indicated that the gga-miR-15 family, gga-miR-103-3p, gga-miR-456-3p, and gga-miR-27b-3p, as core differentially expressed miRNAs, may potentially regulate multiple genes which are involved in immune-related pathways; and that the core genes Suppressor of IKBKE 1 (SIKE1) and high mobility group AT-hook 2 (HMGA2) are associated with the miR-17 family (gga-miR-20a-5p, gga-miR-20b-5p, gga-miR-106-5p, and gga-miR-17-5p) and gga-let -7 family (gga-let-7b, gga-let-7i, gga-let-7c-5p, and gga-let-7f-5p). The interaction networks of mRNAs of significantly enrichment pathways and PPI (protein-protein interaction) networks showed that IL6, IL1B, IL8L1, CCL5, SOCS3, SOCS1, ITGB5, GSTA3, SQLE, FDFT1, FN1, IL18, IL10, MAPK11 and MAPK12 are network core nodes and that most of them are strongly associated with immune response. One of the candidate miRNAs, gga-miR-20b-5p, may play an important role in stress-induced immunosuppression. Luciferase assay and over-expression experiments suggested that gga-miR-20b-5p negatively regulated the expression of target gene SIKE1. These results provide better understanding of the mechanism of stress-induced immunosuppression in Gushi chicken bursa, and provide novel targets for subsequent research to improve poultry anti-stress capability.
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http://dx.doi.org/10.1016/j.molimm.2021.02.022DOI Listing
June 2021

Efficient preparation of dual-emission ratiometric fluorescence sensor system based on aptamer-composite and detection of bis(2-ethylhexyl) phthalate in pork.

Food Chem 2021 Aug 20;352:129352. Epub 2021 Feb 20.

Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address:

A ratiometric fluorescence sensor system is proposed for detecting bis(2-ethylhexyl) phthalate (DEHP) in pork, which is based on aptamer recognition with molybdenum disulfide quantum dots and cadmium telluride quantum dots (MoS QDs/CdTe-Apta). Two signals exist in the system, among which the response signal is transmitted by CdTe-Apta. The amide condensation between aptamers and CdTe QDs shortens the distance between CdTe QDs and DEHP, thus quenching the fluorescence of CdTe QDs, possibly through a photoinduced electron transfer mechanism. The MoS QDs deliver the self-calibration signal, and the fluorescence of MoS QDs remains almost constant when co-existing with DEHP. Linearity (R = 0.9536) was established for the DEHP concentration range 0.005-3.0 mg·L, with a limit of detection of 0.21 μg·L. The system was successfully applied in the determination of DEHP in pork. The system has potential for the quantitative determination of DEHP in practical applications.
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http://dx.doi.org/10.1016/j.foodchem.2021.129352DOI Listing
August 2021

Genome mining combined metabolic shunting and OSMAC strategy of an endophytic fungus leads to the production of diverse natural products.

Acta Pharm Sin B 2021 Feb 5;11(2):572-587. Epub 2020 Aug 5.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.

Endophytic fungi are promising producers of bioactive small molecules. Bioinformatic analysis of the genome of an endophytic fungus revealed 43 biosynthetic gene clusters, exhibited its strong ability to produce numbers of secondary metabolites. However, this strain mainly produce rubratoxins alone with high yield in varied culture conditions, suggested most gene clusters are silent. Efforts for mining the cryptic gene clusters in , including epigenetic regulation and one-strain-many-compounds (OSMAC) approach were failed probably due to the high yield of rubratoxins. A metabolic shunting strategy by deleting the key gene for rubratoxins biosynthesis combining with optimization of culture condition successfully activated multiple silent genes encoding for other polyketide synthases (PKSs), and led to the trace compounds detectable. As a result, a total of 23 new compounds including azaphilone monomers, dimers, trimers with unprecedented polycyclic bridged heterocycle and spiral structures, as well as siderophores were identified. Some compounds showed significant cytotoxicities, anti-inflammatory or antioxidant activities. The attractive dual PKSs gene clusters for azaphilones biosynthesis were mined by bioinformatic analysis and overexpression of a pathway specific transcription factor. Our work therefor provides an efficient approach to mine the chemical diversity of endophytic fungi.
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http://dx.doi.org/10.1016/j.apsb.2020.07.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893140PMC
February 2021
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