Publications by authors named "Wenting Deng"

12 Publications

  • Page 1 of 1

Development and validation of a novel ferroptosis-related gene signature for predicting prognosis and immune microenvironment in head and neck squamous cell carcinoma.

Int Immunopharmacol 2021 Jun 12;98:107789. Epub 2021 Jun 12.

Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China; Department of Hearing and Speech Science, Xinhua College, Sun Yat-Sen University, Guangzhou, China. Electronic address:

Ferroptosis plays an important role across variable cancer types. However, few studies have focused on the prognostic patterns of ferroptosis-related genes in HNSCC. Cohorts with mRNA expression profiles, as well as corresponding clinical data of HNSCC patients from published studies, were collected and consolidated from public databases. We performed random survival forest analysis, Kaplan-Meier (KM) analysis of best combinations, and Cox regression analysis on 231 ferroptosis-related genes to construct a gene signature in the discovery cohort (TCGA), and later validated it in the validation cohort (GEO). The 7-gene signature was constructed to stratify patients into two groups according to their level of risk. Poorer overall survival (OS) was detected in the high risk (HRisk) group than in the low risk (LRisk) group in both the TCGA cohort (P < 0.0001, HR = 1.71, 95%CI:1.41-2.07) and the GEO cohort (P < 0.001, HR = 1.68, 95%CI:1.32-2.13). The risk score was identified as an independent predictive factor of OS in multivariate Cox regression analyses (HR > 1, P < 0.0001) in both cohorts. The signature's predictive capacity was proven by the time-dependent receiver operating characteristic (ROC) curve analysis and nomogram analysis. Functional enrichment analysis revealed that immunosuppressive pathways such as matrix extracellular space, and (transforming growth factor-β)TGF-β were enriched. The HRisk group was strongly associated with upregulation of both cancer-related pathways and stromal scores, while higher proportions of anti-tumor immune cells and immune signatures were enriched in the LRisk group. In conclusion, the signature based on 7 ferroptosis-related genes could be applicable for predicting the prognosis of HNSCC, indicating that ferroptosis may be a potential therapeutic target for HNSCC.
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http://dx.doi.org/10.1016/j.intimp.2021.107789DOI Listing
June 2021

Efficacy of Repetitive Transcranial Magnetic Stimulation (rTMS) for Tinnitus: A Retrospective Study.

Ear Nose Throat J 2021 Jun 15:1455613211016896. Epub 2021 Jun 15.

Department of Hearing and Speech Science, Xinhua College, Sun Yat-sen University, Guangzhou, China.

Objective: Current studies still find insufficient evidence to support the routine use of repetitive transcranial magnetic stimulation (rTMS) in tinnitus. This study aimed to assess response of tinnitus to treatment with rTMS and identify factors influencing the overall response.

Methods: Between January 2016 and May 2017, 199 tinnitus patients were identified from a retrospective review of the electronic patient record at the Sun Yat-sen Memorial Hospital. All patients received rTMS treatment. Their clinicodemographic profile and outcomes, including the tinnitus handicap inventory (THI) and visual analog scale (VAS) scores, were extracted for analysis.

Results: Regarding the THI results, 62.3% of all patients responded to rTMS. The analysis of the VAS score revealed an overall response rate of 66.3%. Both percentages were close to the patient's subjective assessment result, of 63.8%. Patients with tinnitus of less than 1-week duration had the highest response rate to rTMS in terms of either THI/VAS scores or the patient's subjective assessment of symptoms. Tinnitus duration was recognized as a factor influencing the overall response to the treatment.

Conclusions: Repetitive transcranial magnetic stimulation treatment is effective for patients with tinnitus, but its efficacy is affected by tinnitus duration. Tinnitus patients are advised to attend for rTMS as soon as possible since therapy was more effective in those with a shorter duration of disease of less than 1 week.
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http://dx.doi.org/10.1177/01455613211016896DOI Listing
June 2021

Alcohol consumption and risk of stomach cancer: A meta-analysis.

Chem Biol Interact 2021 Feb 4;336:109365. Epub 2021 Jan 4.

National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Stomach cancer is one of the most common cancers in the world. The relationship between alcohol consumption and the risk of stomach cancer remains unclear. Epidemiology studies investigating this relationship have shown inconsistent findings. A meta-analysis was performed to explore the association between alcohol consumption and increased stomach cancer risk. Eighty-one epidemiology studies, including 68 case-control studies and 13 cohort studies, were included in this study. A significant association was found between alcohol consumption and increased risk of stomach cancer (OR = 1.20, 95% CI 1.12-1.27). To explore the source of the significant heterogeneity (p < 0.05, I = 86%), analysis was stratified by study type (case-control study and cohort study), control type (hospital-based control and population-based control), gender (male, female, and mix), race (White and Asian), region (United States, Sweden, China, Japan), subsite of stomach cancer, and type of alcohol. The stratified analyses found that region and cancer subsite are major sources of the high heterogeneity. The inconsistent results in different regions and different subsites might be related to smoking rates, Helicobacter pylori infection, obesity, and potential genetic susceptibility. The positive association between drinking and increased risk of stomach cancer is consistent in stratified analyses. The dose-response analysis showed a clear trend that a higher daily intake of alcohol is associated with a higher risk of stomach cancer.
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http://dx.doi.org/10.1016/j.cbi.2021.109365DOI Listing
February 2021

-Substituted Thiazole Orange for Selective Fluorescence Detection to G-Quadruplex DNA and Molecular Docking Simulation.

ACS Omega 2020 Oct 2;5(40):26056-26062. Epub 2020 Oct 2.

Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, National Demonstration Center for Experimental Chemistry Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, P. R. China.

The cyanine dye thiazole orange (TO, including 2TO and 4TO) is widely used as a light-up fluorescent probe upon binding to almost all forms of DNA, but it exhibits poor selectivity for recognizing G-quadruplex DNA (G-DNA), which has significant biological functions in biological processes and therapeutic applications. Here, introducing benzyl substituent to the position of the methine chain of 2TO is expected to selectively recognize G-DNA. The spectroscopic titrations reveal that modified 2TO (-Bn-2TO) has almost no background fluorescence in solution and shows a preference to bind with G-DNA over ssDNA, dsDNA, and ct-DNA. Specifically, -Bn-2TO displays a strong fluorescent signal upon interaction with G-DNA and a very weak fluorescent signal upon interaction with ssDNA, dsDNA, and ct-DNA, displaying considerable selectivity for G-DNA. However, parent 2TO all gives a fluorescent signal in G-DNA, dsDNA, and ct-DNA. The fluorescence intensity of increases nearly 80-162 times when bound with different G-DNA. The binding constants of and 2TO to G-DNA htg22 are 3.16 and 1.52 μM, respectively. Molecular docking study of and 2TO with different DNA reveals that introducing benzyl substituent to the meso position methine chain of 2TO alters the planarity of the chromophore, thus enhancing the interactions with G-DNA and weakening the interactions with duplex DNA and therefore realizing selective detection to G-DNA.
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http://dx.doi.org/10.1021/acsomega.0c03556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7557940PMC
October 2020

MiR-21b-3p protects NS2OY cells against oxygen-glucose deprivation/reperfusion-induced injury by down-regulating cyclooxygenase-2.

Am J Transl Res 2019 15;11(5):3007-3017. Epub 2019 May 15.

Department of Orthopaedics, General Hospital of Southern Theater Command, PLA Guangzhou, Guangdong, People's Republic of China.

Recent studies have shown abnormal expression levels of cyclooxygenase-2 (COX-2) and miR-21b-3p in cerebral ischemia-reperfusion (I/R) rat models. Decreased COX-2 expression could reduce brain injury and thus could be a target of miR-21b-3p according to the miRNA databases (miRDB) analysis. However, its functions and underlying mechanisms in I/R injury remain unclear. In our study, we have established an oxygen/glucose deprivation and reperfusion (OGD/R) model by using NS2OY cells. The expression of miR-21b-3p and COX-2 was determined by quantitative real-time PCR or Western blot, and the fluorescence intensities were detected by fluorescence in situ hybridization (FISH) or immunofluorescence. After transfection and OGD/R treatments, the functions of miR-21b-3p and COX-2 on cell viability and apoptosis were detected using cell-counting kit 8, Edu staining, flow cytometry and Hoechst staining, respectively. Finally, dual-luciferase reporter assay was used to explore the relationship between miR-21-b-3p and COX-2. The results have showed that COX-2 mRNA and protein expression were significantly increased; however, the expression of miR-21b-3p was remarkably reduced in NS2OY cells after OGD/R treatment. The changes were most remarkable in OGD 2 h/R24 group. Function analysis has showed that when NS2OY cells were exposed to OGD/R injury, overexpressed miR-21b-3p significantly downregulated COX-2 expression, increased cell viability and decreased apoptosis. In addition, knocking down the expression of COX-2 could also increase cell viability and decrease apoptosis. Dual-luciferase reporter assays showed miR-21b-3p as the target of 3'-UTR of COX-2. Therefore, we concluded that OGD/R-induced injury by down-regulating COX-2.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556624PMC
May 2019

Meso-Substituent-Directed Aggregation Behavior and Water Solubility: Direct Functionalization of Methine Chain in Thiazole Orange and Biological Applications in Aqueous Buffer.

J Org Chem 2019 04 13;84(7):3960-3967. Epub 2019 Mar 13.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, National Demonstration Center for Experimental Chemistry Education, College of Chemistry and Materials Science , Northwest University , Xi'an 710127 , P.R. China.

A new strategy is presented to preclude aggregation and enhance water solubility of cyanine dyes. Namely, a heteroatom-containing substituent, for distorting molecular plane and increasing interaction with water molecules, is introduced to the methine chain of 2-thiazole orange (1, a monocyanine) via one-step, and 2-thiazole orange derivatives 2a-g are prepared accordingly. The X-ray crystal structures show that the molecular plane of 2a-g is drastically twisted, which reduces intermolecular π-π stacking. The derivatives 2a-g exhibit good to excellent water solubility and can be dissolved in aqueous phosphate-buffered saline (PBS) at concentrations suitable for biomedical applications. No aggregation in aqueous PBS, relatively high molar extinction coefficients, and low solvatochromism of 2a-g are reflected by the UV-vis spectra. Compound 2b shows fast response and high selectivity for biothiols (Cys, Hcy, and GSH) in aqueous PBS and is further employed to detect endogenous biothiols with decent biocompatibility as demonstrated by live cell fluorescence imaging.
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http://dx.doi.org/10.1021/acs.joc.8b03122DOI Listing
April 2019

Light-Driven Reversible Intermolecular Proton Transfer at Single-Molecule Junctions.

Angew Chem Int Ed Engl 2019 Mar 14;58(12):3829-3833. Epub 2019 Feb 14.

State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, iChEM, Xiamen University, Xiamen, 361005, China.

Photoresponsive molecular systems are essential for molecular optoelectronic devices, but most molecular building blocks are non-photoresponsive. Employed here is a photoinduced proton transfer (PIPT) strategy to control charge transport through single-molecule azulene junctions with visible light under ambient conditions, which leads to a reversible and controllable photoresponsive molecular device based on non-photoresponsive molecules and a photoacid. Also demonstrated is the application of PIPT in two single-molecule AND gate and OR gate devices with electrical signal as outputs.
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http://dx.doi.org/10.1002/anie.201813137DOI Listing
March 2019

Scutellarin inhibits human renal cancer cell proliferation and migration via upregulation of PTEN.

Biomed Pharmacother 2018 Nov 5;107:1505-1513. Epub 2018 Sep 5.

College of Pharmacy, Xi'an Medical University, No. 1 Xinwang Road of Weiyang District, 710021, Xi'an, Shaanxi, China.

Background: Scutellarin is a naturally flavone glycoside that has been shown to exhibit anti-proliferative and anti-apoptotic activities among various human malignancies. However, the anti-cancer effect of Scutellarin in Renal cell carcinoma (RCC) and the underlying mechanism remains unclear.

Methods And Materials: RCC cell lines ACHN and 786-O were treated with different concentrations (0-210 μM) of Scutellarin in vitro. Cell viability and proliferation were investigated by MTT and colony formation assays. Cell invasion and migration were detected by Transwell assays. Cell apoptosis and cell cycle distribution was measured by flow cytometry. Western blot was used to investigate the expression levels of crucial proteins. Xenograft tumor model was established to evaluate tumor growth in vivo.

Results: Scutellarin significantly inhibited RCC cell proliferation in a dose- and time- dependent manner. Treatment of RCC cells with Scutellarin (30, 60, and 90 μM) markedly induced apoptosis and cell cycle arrested at G0/G1 phase in a concentration-dependent characteristic. Cell invasion and migration capacities of RCC cells were also dose-dependently suppressed by Scutellarin treatment. Western blot assays revealed that the crucial proteins including cyclin D1, CDK2, Bcl2, MMP-2, and MMP-9 were significantly reduced while Bax, cleaved caspase 3 and p21 were increased by Scutellarin in RCC cells. In vivo assay indicated that Scutellarin possessed anti-cancer effect on xenograft without triggering toxic effect. Mechanically, Scutellarin dramatically increased the protein level of phosphatase and tensin homologue (PTEN) and inhibited the activity of P13K/AKT/mTOR signaling. Ectopic expression of PTEN enhanced the inhibitory effect of Scutellarin on RCC proliferation while knockdown of PTEN abrogated it through regulating its downstream P13K/AKT/mTOR signaling pathway.

Conclusion: Scutellarin inhibited RCC cell proliferation and invasion partially by enhancing the expression of PTEN through inhibition of P13K/AKT/mTOR pathway, suggesting that Scutellarin might serve as a potential therapeutic agent in RCC treatment.
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http://dx.doi.org/10.1016/j.biopha.2018.08.127DOI Listing
November 2018

Approach to Introducing Substituent on the Dipole Conjugate Chain: The D-π-A Methine Chain Electrophilic Substitution.

Org Lett 2018 01 7;20(1):60-63. Epub 2017 Dec 7.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, College of Chemistry and Materials Science, Northwest University , Xi'an 710127, P. R. China.

The D-π-A methine chain electrophilic substitution reactions for directly introducing some functional groups to the dipole conjugate chain, with mild conditions and good yields, are disclosed. This method is shown to efficiently replace the hydrogen on the D-π-A methine chain with an electrophile to synthesize methine-substituted Cy3.5 in one step.
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http://dx.doi.org/10.1021/acs.orglett.7b03345DOI Listing
January 2018

Danhong enhances recovery from residual dizziness after successful repositioning treatment in patients with benign paroxysmal positional vertigo.

Am J Otolaryngol 2014 Nov-Dec;35(6):753-7. Epub 2014 Jul 9.

Department of Internal Medicine, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, China. Electronic address:

Objectives: Although the repositioning maneuvers are usually very effective in patients with BPPV, some patients still complain residual dizziness. Danhong injection (DHI), a traditional Chinese medicine, can effectively dilate blood vessels and improve microcirculation, and has been proven to be effective in improving cervical vertigo and posterior circulation ischemic vertigo. The aim of this study was to evaluate the effects of DHI on residual dizziness after successful repositioning treatment in patients with BPPV.

Methods: Eighty-six patients with BPPV were randomized into two treatment groups, DHI group and non DHI group. The DHI group received the same repositioning treatment as the non-DHI group, with the addition of DHI therapy. The durations of residual dizziness of DHI group and non-DHI group were compared. In addition, the scores of the dizziness handicap inventory of these two groups were calculated.

Results: The durations of residual dizziness of DHI group were shorter than that of non-DHI group. There were no significant differences in the scores of dizziness handicap inventory in the first week between these two groups, and there were much significant differences in the second, the fourth, the sixth and eighth weeks.

Conclusions: The results demonstrate that DHI can significantly improve the residual dizziness after successful repositioning treatment in patients with BPPV.
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http://dx.doi.org/10.1016/j.amjoto.2014.07.001DOI Listing
August 2015

Radix astragali inhibits the down-regulation of connexin 26 in the stria vascularis of the guinea pig cochlea after acoustic trauma.

Eur Arch Otorhinolaryngol 2015 Sep 25;272(9):2153-60. Epub 2014 May 25.

Department of Otolaryngology, Guangzhou General Hospital of Guangzhou Military Command, Liu Hua Road 111, Guangzhou, 510010, China,

Connexin 26 (cx26) plays an important role in the intercellular signaling and is related to K(+) metabolism in stria vascularis (SV). Reactive oxygen species (ROS) are negative regulators of cx26, reducing intercellular coupling in cochlea. ROS plays an important role in acoustic trauma. Radix astragali is a natural antioxidant that decreases impulse noise-induced hearing loss through its ability to inhibit ROS. The purpose of this study was to investigate if radix astragali has the potential to reduce the change of cx26 in SV from impulse noise. Guinea pigs in the experimental group were administered radix astragali intraperitoneally. Auditory thresholds were assessed by sound-evoked auditory brainstem response (ABR) at click and tone bursts of 8, 16 and 32 kHz, 24 h before and 72 h after exposure to impulse noise. 4-Hydroxynonenal, cx26 and KCNQ1 were determined immunohistochemically in SV. SV was analyzed by transmission electron microscopy. Radix astragali significantly reduced the ABR deficits and the SV damage, and decreased the shifts of the expression of cx26 and KCNQ1 in the SV. These results suggest that the beneficial effect of radix astragali on impulse noise-induced hearing loss may be also due to its ability to reduce the change of cx26 in SV.
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http://dx.doi.org/10.1007/s00405-014-3093-4DOI Listing
September 2015

Tap water nasal irrigation in adults with seasonal allergic rhinitis: a randomized double-blind study.

Eur Arch Otorhinolaryngol 2014 Jun 4;271(6):1549-52. Epub 2013 Oct 4.

Department of Otolaryngology, Guangzhou General Hospital of Guangzhou Military Command, Liu Hua Road 111, Guangzhou, 510010, China,

Saline nasal irrigation is effective in the treatment of seasonal allergic rhinitis, and sodium chloride itself has no antiallergic effects. The mechanism of saline nasal irrigation depends mainly on washing away allergens and inflammatory mediators induced by allergic reactions. Tap water has the same washing effects as saline. In this study, it was investigated if tap water nasal irrigation was effective in the treatment of seasonal allergic rhinitis. Sixty-four patients diagnosed with seasonal allergic rhinitis were enrolled. Patients were randomized to tap water nasal irrigation group and non-tap water nasal irrigation group for treatment. Patients of both groups were treated with desloratadine. Treatment outcomes were measured using allergic rhinitis Quality of Life (QoL) survey was completed at baseline and after 3 weeks of therapy. There were statistically significant differences in QoL scores between tap water nasal irrigation group and non-tap water nasal irrigation group. The tap water nasal irrigation group had better QoL scores than the non-tap water nasal irrigation group. Tap water nasal irrigation can be a valuable adjuvant therapy for patients with seasonal allergic rhinitis.
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http://dx.doi.org/10.1007/s00405-013-2741-4DOI Listing
June 2014
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