Publications by authors named "Wentian Zhang"

12 Publications

  • Page 1 of 1

The Prognostic Value of Tumor Mutation Burden and Immune Cell Infiltration in Thymic Epithelial Tumors.

Ann Clin Lab Sci 2021 Jan;51(1):44-54

Department of Thoracic Surgery, Ruijin Hospital; Shanghai Jiao Tong University School of Medicine, Shanghai, China

Objective: The question of whether the tumor mutation burden (TMB) is associated with either improved survival outcomes or improvement of immunotherapies remains controversial in various malignancies. The aim of this study is to investigate the genomic landscape of the relationship between TMB and immune cell infiltration in thymic epithelial tumors (TETs).

Methods: We downloaded somatic mutation data, transcriptome sequencing data, and clinical information of TETs from the Cancer Genome Atlas (TCGA) database. We assessed the abundance of 22 immune fractions between low-TMB (TMB-L) and high-TMB (TMB-H) groups using the "CIBERSORT" package.

Results: Missense mutation had the highest frequency of mutation among the nine variant classifications in TETs. Higher TMB levels were associated with poor survival outcomes (<0.05), and higher Masaoka stages (<0.05). More importantly, TMB levels were much higher in the thymic cancer than in thymoma (<0.01). The infiltration levers of naive CD4(+) T cells and regulatory T cells were significantly higher in the TMB-L group than in the TMB-H group, and this was further associated with better overall survival (OS) in patients with TETs.

Conclusion: The present study indicates that the prognosis of TMB-H patients with TETs is significantly poorer than is that of TMB-L patients, which might result from the different levels of infiltration of naive CD4(+) T cells and regulatory T cells.
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January 2021

One-Class Fingerprint Presentation Attack Detection Using Auto-Encoder Network.

IEEE Trans Image Process 2021 28;30:2394-2407. Epub 2021 Jan 28.

Automated Fingerprint Recognition Systems (AFRSs) have been threatened by Presentation Attack (PA) since its existence. It is thus desirable to develop effective presentation attack detection (PAD) methods. However, the unpredictable PAs make PAD be a challenging problem. This paper proposes a novel One-Class PAD (OCPAD) method for Optical Coherence Technology (OCT) images based fingerprint PA detection. The proposed OCPAD model is learned from a training set only consists of Bonafides (i.e. real fingerprints). The reconstruction error and latent code obtained from the trained auto-encoder network in the proposed model is taken as the basis for the following spoofness score calculation. To get more accurate reconstruction error, we propose an activation map based weighting model to further refine the accuracy of reconstruction error. We test different statistics and distance measures and finally use a decision level fusion to make the final prediction. Our experiments are performed using a dataset with 93200 bonafide scans and 48400 PA scans. The results show that the proposed OCPAD can achieve a True Positive Rate (TPR) of 99.43% when the False Positive Rate (FPR) equals to 10% and a TPR of 96.59% when FPR=5%, which significantly outperformed a feature based approach and a supervised learning based model requiring PAs for training.
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http://dx.doi.org/10.1109/TIP.2021.3052341DOI Listing
January 2021

Dual inhibition of HDAC and tyrosine kinase signaling pathways with CUDC-907 attenuates TGFβ1 induced lung and tumor fibrosis.

Cell Death Dis 2020 09 17;11(9):765. Epub 2020 Sep 17.

Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Rd, Shanghai, 200025, China.

TGFβ1 signaling is a critical driver of collagen accumulation in pulmonary fibrotic diseases and a well-characterized regulator of cancer associated fibroblasts (CAF) activation in lung cancer. Myofibroblasts induced by TGFβ1 and other factors are key players in the pathogenesis of lung fibrosis and tumor. Tremendous attention has been gained to targeting myofibroblasts in order to inhibit the progression of fibrosis and myofibroblast-induced tumor progression and metastasis. Here we determined the therapeutic efficacy of simultaneously targeting PI3K and HDAC pathways in lung myofibroblasts and CAF with a single agent and to evaluate biomarkers of treatment response. CUDC-907 is a first-in-class compound, functioning as a dual inhibitor of HDACs and PI3K/AKT pathway. We investigated its effects in counteracting the activity of TGFβ1-induced myofibroblasts/CAF in regard to cell proliferation, migration, invasion, apoptosis in vitro antifibrosis efficiency in vivo. We found that CUDC-907 inhibited myofibroblasts/CAF cell proliferation, migration and apoptosis in a dose-dependent manner and caused cell cycle arrest at G1-S phase. CUDC-907 not only inhibited myofibroblasts markers expression, but also significantly inhibited the phosphorylation level of AKT, mTOR, Smad2/3, and promoted acetylation of histones. Furthermore, the observed inhibitory effect was also confirmed in bleomycin-induced mice lung fibrosis and nude mouse transplanted tumor model. Overall, these data suggest that dual inhibition of HDAC and the tyrosine kinase signaling pathways with CUDC-907 is a promising treatment strategy for TGFβ1-induced lung and tumor fibrosis.
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http://dx.doi.org/10.1038/s41419-020-02916-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499263PMC
September 2020

Air Quality Monitoring for Vulnerable Groups in Residential Environments Using a Multiple Hazard Gas Detector.

Sensors (Basel) 2019 Jan 17;19(2). Epub 2019 Jan 17.

Centre for Health Technologies, School of Biomedical Engineering, Faculty of Engineering and Information Technology, University of Technology Sydney, Sydney, NSW 2007, Australia.

This paper presents a smart "e-nose" device to monitor indoor hazardous air. Indoor hazardous odor is a threat for seniors, infants, children, pregnant women, disabled residents, and patients. To overcome the limitations of using existing non-intelligent, slow-responding, deficient gas sensors, we propose a novel artificial-intelligent-based multiple hazard gas detector (MHGD) system that is mounted on a motor vehicle-based robot which can be remotely controlled. First, we optimized the sensor array for the classification of three hazardous gases, including cigarette smoke, inflammable ethanol, and off-flavor from spoiled food, using an e-nose with a mixing chamber. The mixing chamber can prevent the impact of environmental changes. We compared the classification results of all combinations of sensors, and selected the one with the highest accuracy (98.88%) as the optimal sensor array for the MHGD. The optimal sensor array was then mounted on the MHGD to detect and classify the target gases without a mixing chamber but in a controlled environment. Finally, we tested the MHGD under these conditions, and achieved an acceptable accuracy (70.00%).
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http://dx.doi.org/10.3390/s19020362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359352PMC
January 2019

NOS.E: A New Fast Response Electronic Nose Health Monitoring System.

Annu Int Conf IEEE Eng Med Biol Soc 2018 Jul;2018:4977-4980

We present a practical electronic nose (e-nose) sys-tem, NOS.E, for the rapid detection and identification of human health conditions. By detecting the changes in the composition of an individual's respiratory gases, which have been shown to be linked to changes in metabolism, e-nose systems can be used to characterize the physical health condition. We demonstrated our system's viability with a simple data set consists of breath collected under three different scenarios from one volunteer. Our preliminary results show the popular classifier SVM can discriminate NOS.E's responses under the three scenarios with high performance. In future work, we will aim to gather a more varied data set to test NOS.E's abilities.
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http://dx.doi.org/10.1109/EMBC.2018.8513416DOI Listing
July 2018

Neoadjuvant PD-1 blockade in non-small cell lung cancer: what else do we need to do?

J Thorac Dis 2018 Sep;10(Suppl 26):S3162-S3165

Department of Radiation Oncology, Thomas Jefferson University, Philadelphia, PA, USA.

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http://dx.doi.org/10.21037/jtd.2018.07.84DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186643PMC
September 2018

c-kit(+)AT2R(+) Bone Marrow Mononuclear Cell Subset Is a Superior Subset for Cardiac Protection after Myocardial Infarction.

Stem Cells Int 2016 27;2016:4913515. Epub 2016 Jun 27.

Department of Cardiovascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, No. 160 Pu-Jian Road, Shanghai 200127, China.

Although the bone marrow mononuclear cell (BMMNC) is known as an ideal cell type for cell-based therapy for MI treatment, the effective subpopulation still remains unknown. Our study aimed at identifying the optimal subset of BMMNCs suited for cardiac regeneration. In this study, we observed that MI led to (i) a significant increase of the c-kit(+)AT2R(+) BMMNC subpopulation in mice and (ii) a modest increase of AT2R(+) BMMNCs in humans. c-kit(+)AT2R(+) and c-kit(+)AT2R(-) BMMNC subpopulations were obtained from mice after MI. Then, we cocultured cardiac H9C2 cells with c-kit(+)AT2R(+), c-kit(+)AT2R(-), and unfractionated BMMNCs; finally, we found that the c-kit(+)AT2R(+) subset is superior to the c-kit(+)AT2R(-) subset in improving cardiomyocyte protection in vitro. Of note, c-kit(+)AT2R(+) BMMNCs showed a more robust migration capacity than c-kit(+)AT2R(-) and unfractionated BMMNCs in vitro and in vivo. Additionally, compared to c-kit(+)AT2R(-) and unfractionated BMMNCs, intravenous transplantation of c-kit(+)AT2R(+) BMMNC resulted in smaller infarct size and lower levels of inflammatory reactions in heart tissue, leading to a higher global heart function improvement. In conclusion, our results indicate that the c-kit(+)AT2R(+) BMMNC subpopulation exerts a protective effect against MI and shows promising therapeutic possibilities with regard to the treatment of ischemic heart disease.
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http://dx.doi.org/10.1155/2016/4913515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4939348PMC
July 2016

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro.

Cell Physiol Biochem 2016 23;38(6):2285-99. Epub 2016 May 23.

Background/aims: Adipose-derived stem cells (ASCs) belong to mesenchymal stem cells and may play a potential role as seeding cells in stem cell transplantation. To be able to exploit stem cells as therapeutic tool, their defects in some important cellular functions, such as low survival rate and cellular activity, should be considered. This is especially the case for stem cells that are intended for transplantation. Of note, stem cell responses to hormones should be considered since estrogen is known to play a critical role in stem cell behavior. However, different impacts of the estrogen receptor (ER) types α and β have not been fully determined in ASC function. In this study, we investigated effects of ERα and ERβ on ASC proliferation, migration, as well as in adipogenesis.

Methods: ASCs obtained from mice were cultured with 100nM ERα or ERβ agonist PPT and DPN, respectively. The ERα and ERβ antagonist ICI 182,780 (100nM) was used as control.

Results: Compared to ERβ, ERα appears more potent in improving ASC proliferation and migration. Investigation of adipogenesis revealed that ERβ played a significant role in suppressing ASC-mediated brown tissue adipogenesis which is in contrast to ERα. These results correlated with reduced mRNA expression of UCP-1, PGC-1α and PPAR-x03B3;.

Conclusions: ERα plays a more critical role in promoting ASC proliferation and migration while ERβ is more potent in suppressing ASC brown adipose tissue differentiation mediated by decreased UCP-1, PGC-1α and PPAR-x03B3; expression.
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http://dx.doi.org/10.1159/000445583DOI Listing
February 2017

Electrospun nanofibrous sheets of collagen/elastin/polycaprolactone improve cardiac repair after myocardial infarction.

Am J Transl Res 2016 15;8(4):1678-94. Epub 2016 Apr 15.

Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.

Electrospun nanofibrous sheets get increasing attention in myocardial infarction (MI) treatment due to their good cytocompatibility to deliver transplanted stem cells to infarcted areas and due to mechanical characteristics to support damaged tissue. Cardiac extracellular matrix is essential for implanted cells since it provides the cardiac microenvironment. In this study, we hypothesized high concentrations of cardiac nature protein (NP), namely elastin and collagen, in hybrid polycaprolactone (PCL) electrospun nanofibrous sheets could be effective as cardiac-mimicking patch. Optimal ratio of elastin and collagen with PCL in electrospun sheets (80% NP/PCL) was selected based on cytocompatibility and mechanical characteristics. Bone-marrow (BM) c-kit(+) cells anchoring onto NP/PCL sheets exhibited increased proliferative capacity compared with those seeded on PCL in vitro. Moreover, we examined the improvement of cardiac function in MI mice by cell-seeded cardiac patch. Green Fluorescent Protein (GFP)-labeled BM c-kit(+) cells were loaded on 80% NP/PCL sheets which was transplanted into MI mice. Both 80% NP/PCL and c-kit(+)-seeded 80% NP/PCL effectively improved cardiac function after 4 weeks of transplantation, with reduced infarction area and restricted LV remodeling. C-kit(+)-seeded 80% NP/PCL was even superior to the 80% NP/PCL alone and both superior to PCL. GFP(+) cells were identified both in the sheets and local infarcted area where transplanted cells underwent cardiac differentiation after 4 weeks. To the best of our knowledge, this is the first report that sheets with high concentrations of nature proteins loaded with BM c-kit(+) cells might be a novel promising candidate for tissue-engineered cardiac patch to improve cardiac repair after MI.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859897PMC
May 2016

Role and Variation of the Amount and Composition of Glomalin in Soil Properties in Farmland and Adjacent Plantations with Reference to a Primary Forest in North-Eastern China.

PLoS One 2015 2;10(10):e0139623. Epub 2015 Oct 2.

Research Institute of Forest Ecology and Forestry Ecological Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.

The glycoprotein known as glomalin-related soil protein (GRSP) is abundantly produced on the hyphae and spores of arbuscular mycorrhizal fungi (AMF) in soil and roots. Few studies have focused on its amount, composition and associations with soil properties and possible land-use influences, although the data hints at soil rehabilitation. By choosing a primary forest soil as a non-degraded reference, it is possible to explore whether afforestation can improve degraded farmland soil by altering GRSP. In this paper, close correlations were found between various soil properties (soil organic carbon, nitrogen, pH, electrical conductivity (EC), and bulk density) and the GRSP amount, between various soil properties and GRSP composition (main functional groups, fluorescent substances, and elements). Afforestation on farmland decreased the EC and bulk density (p < 0.05). The primary forest had a 2.35-2.56-fold higher GRSP amount than those in the plantation forest and farmland, and GRSP composition (tryptophan-like and fulvic acid-like fluorescence; functional groups of C-H, C-O, and O-H; elements of Al, O, Si, C, Ca, and N) in primary forest differed from those in plantation forest and farmland (p < 0.05). However, no evident differences in GRSP amount and composition were observed between the farmland and the plantation forest. Our finding highlights that 30 years poplar afforestation on degraded farmland is not enough to change GRSP-related properties. A longer period of afforestation with close-to-nature managements may favor the AMF-related underground recovery processes.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139623PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592192PMC
June 2016

Membrane estrogen receptor alpha is an important modulator of bone marrow C-Kit+ cells mediated cardiac repair after myocardial infarction.

Int J Clin Exp Pathol 2015 1;8(5):4284-95. Epub 2015 May 1.

Renji-Med X Clinical Stem Cell Research Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai 200127, China.

It has been validated that c-kit positive (c-kit+) cells in infarcted myocardium are from bone marrow (BM). Given the recent study that in the heart, estrogen receptor alpha (ERα) is involved in adaptive mechanisms by supporting cardiomyocytes survival via post-infarct cardiac c-kit+ cells, we tested a novel hypothesis that membrane ERα (mERа) supports survival of BM c-kit+ cells and enhance protective paracrine function for cardiac repair. Our data showed that myocardial infarction (MI) leads to an increase in c-kit+ first in bone marrow and then specifically within the infarcted myocardium. Also up-regulated mERа in post-infarct BM c-kit+ cells was found in day 3 post MI. In vitro co-culture system, mERа+ enhances the beneficial effects of BM c-kit+ cells by increasing their viability and reducing apoptosis. Post-infarct c-kit+ mERа+ cells population expresses predominant ERα and holds self-renewal as well as cardiac differentiation potentials after MI. In vivo, BM c-kit+ cells reduced infarct size, fibrosis and improved cardiac function. In conclusion, BM c-kit+ mERа+ exerted significantly cardiac protection after MI. A potential important implication of this study is that the manipulation of BM c-kit+ stem cells with ERа-dependent fashion may be helpful in recovering functional performance after cardiac tissue injury.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502993PMC
April 2016

Comparison of the safety of right internal mammary artery versus radial artery for the second arterial conduit during coronary artery bypass grafting.

Heart Lung Circ 2014 Dec 23;23(12):1179-86. Epub 2014 Jun 23.

Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China. Electronic address:

Objective: To compare the safety of procedure in patients receiving right internal mammary artery (RIMA) versus radial artery (RA) as the second arterial graft during left internal mammary artery bypass surgery.

Methods: A literature search was performed, and observational studies comparing RA and RIMA as a second arterial conduit were included. The end points included in-hospital mortality, sternal wound infection (SWI), myocardial infarction (MI), reoperation for bleeding, stroke and low cardiac output syndrome. Sensitivity analysis was performed, and publication bias was assessed.

Results: Analysis of nine studies comprising 1,738 RIMA patients and 1,906 RA patients showed a similar risk of in-hospital mortality (relative risk [RR] 1.13, 95% confidence interval [CI] 0.64 to 1.98) between two groups. The risk of SWI in RIMA patients was significantly higher than that in RA patients (RR 3.19, 95% CI 1.64 to 6.20). The risk of the rest end points did not differ.

Conclusions: The postoperative risk seems to be similar between RIMA group and RA group, except the higher risk of SWI in RIMA patients. The surgeon relies on his own experience with these two arterial grafts and assessment of the patient profile to decide the choice of the second arterial conduit.
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http://dx.doi.org/10.1016/j.hlc.2014.05.017DOI Listing
December 2014