Publications by authors named "Wensheng Wang"

163 Publications

Balancing selection and wild gene pool contribute to resistance in global rice germplasm against planthopper.

J Integr Plant Biol 2021 Jul 24. Epub 2021 Jul 24.

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

Interactions and co-evolution between plants and herbivorous insects are critically important in agriculture. Brown planthopper (BPH) is the most severe insect of rice, and the biotypes adapt to feed on different rice genotypes. Here, we present genomics analyses on 1,520 global rice germplasm for resistance to three BPH biotypes. Genome-wide association studies identified 3,502 single nucleotide polymorphisms (SNPs) and 59 loci associated with BPH resistance in rice. We cloned a previously unidentified gene Bph37 that confers resistance to BPH. The associated loci showed high nucleotide diversity. Genome-wide scans for trans-species polymorphisms revealed ancient balancing selection at the loci. The secondarily evolved insect biotypes II and III exhibited significantly higher virulence and overcame more rice varieties than the primary biotype I. In response, more SNPs and loci evolved in rice for resistance to biotypes II and III. Notably, three exceptional large regions with high SNP density and resistance-associated loci on chromosomes 4 and 6 appear distinct between the resistant and susceptible rice varieties. Surprisingly, these regions in resistant rice might have been retained from wild species Oryza nivara. Our findings expand the understanding of long-term interactions between rice and BPH and provide resistance genes and germplasm resources for breeding durable BPH-resistant rice varieties. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13157DOI Listing
July 2021

Performance Deterioration of Asphalt Mixture under Chloride Salt Erosion.

Materials (Basel) 2021 Jun 17;14(12). Epub 2021 Jun 17.

College of Transportation, Jilin University, Changchun 130025, China.

In order to ensure smooth traffic and driving safety, deicing salt or snow melting agents are usually adopted to solve the problem of traffic jams and prevent pavement surfaces from freezing. The objective of this present study is to investigate the performance deterioration evaluation of asphalt mixture under the chloride salt erosion environment. Five chloride salt solution concentrations were designed and the uniaxial static compression creep test, low-temperature IDT test, freeze-thaw splitting test, and freeze-thaw cycle test were carried out for asphalt mixtures (AC-16) soaked in chloride salt solution. Results showed that with the increase in chloride salt solution concentration, the high-temperature stability, low-temperature crack resistance, and water stability of the asphalt mixture decreases. Moreover, the high-temperature stability, low-temperature crack resistance, and water stability of the asphalt mixture show a decreasing trend under different chloride salt solution concentrations following the negative cubic polynomial function. Based on the viscoelastic analysis, chloride salt solution could reduce the ability of the asphalt mixture to resist instantaneous elastic deformation and permanent deformation, and this influence will become more obvious with the increase in chloride salt solution concentration. In addition, the salt freeze-thaw cycle test indicated that in the early stage of freeze-thaw cycles, the splitting tensile strength of the asphalt mixture decreases rapidly, then tends to be flat, and then decreases rapidly. This study explores the performance damage law of asphalt mixture under salt corrosion, and the analysis results of this study could provide some references for the chloride salt dosage in the snow melting project while spreading deicing salt.
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http://dx.doi.org/10.3390/ma14123339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234858PMC
June 2021

Study on Performance Damage and Mechanism Analysis of Asphalt under Action of Chloride Salt Erosion.

Materials (Basel) 2021 Jun 4;14(11). Epub 2021 Jun 4.

College of Transportation, Jilin University, Changchun 130025, China.

This study aims to investigate the performance evolution and mechanism of asphalt under action of chloride salt erosion. Asphalt samples soaked with five different snow melting chloride salt concentrations were taken as the research object. Then, the high-temperature performance, low-temperature performance, temperature sensitivity and asphalt-aggregate adhesion property of asphalt samples were carried out. Additionally, Fourier transform infrared spectroscopy (FTIR) was used to explore the mechanism of chloride salt erosion on asphalt. Test results showed the linear variation relationships of high-temperature performance, low-temperature performance and temperature sensitivity with chloride salt concentrations. The high-temperature performance of asphalt would be improved by chloride snowmelt salt. With the increase in the chloride salt solution concentration, the low-temperature performance of asphalt became worse, and the temperature sensitivity increased. Moreover, after the effect of the chloride salt solution, the asphalt-aggregate adhesion property decreased with the increase in the chloride salt solution concentration. It is necessary to control the amount of chloride snowmelt salt in the actual snow removal projects. Finally, based on Fourier transform infrared spectroscopy, the mechanism of chloride salt erosion on asphalt was preliminarily explored. With the increase in the chloride salt solution concentration, the proportion of light components (saturated fraction, aromatic fraction) in asphalt decreased, and the proportion of heavy components (resin and asphaltene) with good thermal stability increased.
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http://dx.doi.org/10.3390/ma14113089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200241PMC
June 2021

Chordoid glioma in the thalamus of a child: Rare location and atypical imaging findings.

BJR Case Rep 2021 May 8;7(3):20200108. Epub 2021 Jan 8.

Department of Radiology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China.

Chordoid glioma is a rare intracranial tumour, which usually occurs in middle-aged female patients, mainly in the third ventricle, hypothalamus and suprasellar region. It can reportedly occur in the temporal-parietal lobe, occipital horn of the lateral ventricle and left thalamus. Here, we report a case of chordoid glioma in the thalamic region of a female child, which is different from the previously reported chordoid glioma in the left thalamus. Given its atypical location and imaging findings, it is often misdiagnosed as low-grade glioma before operation. Through the study of this case, we recognized the atypical imaging manifestations of chordoid glioma in a rare location.
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http://dx.doi.org/10.1259/bjrcr.20200108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171145PMC
May 2021

The novel FAT4 activator jujuboside A suppresses NSCLC tumorigenesis by activating HIPPO signaling and inhibiting YAP nuclear translocation.

Pharmacol Res 2021 Jun 9;170:105723. Epub 2021 Jun 9.

Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, PR China; State Key Laboratory of Quality Research in Chinese Medicine/ Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau (SAR), PR China. Electronic address:

FAT atypical cadherin 4 (FAT4) has been identified as a tumor suppressor in lung cancers. However, no agent for lung cancer treatment targeting FAT4 has been used in the clinic. Jujuboside A (JUA) is a major active compound in Semen Ziziphi Spinosae. Semen Ziziphi Spinosae is a traditional Chinese herbal medicine used clinically for tumor treatment to improve patients' quality of life. However, the anti-lung cancer activity and the underlying mechanisms of JUA are not yet fully understood. Here, we demonstrated the anti-lung cancer activity of JUA in two lung cancer mice models and three non-small cell lung cancer (NSCLC) cell lines, and further illustrated its underlying mechanisms. JUA suppressed the occurrence and development of lung cancer and extended mice survival in vivo, and suppressed NSCLC cell activities through cell cycle arrest, proliferation suppression, stemness inhibition and senescence promotion. Moreover, JUA directly bound with and activated FAT4, subsequently activating FAT4-HIPPO signaling and inhibiting YAP nuclear translocation. Knockdown of FAT4 diminished JUA's effects on HIPPO signaling, YAP nuclear translocation, cell proliferation and cellular senescence. In conclusion, JUA significantly suppressed NSCLC tumorigenesis by regulating FAT4-HIPPO-YAP signaling. Our findings suggest that JUA is a novel FAT4 activator that can be developed as a promising NSCLC therapeutic agent targeting the FAT4-HIPPO-YAP pathway.
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http://dx.doi.org/10.1016/j.phrs.2021.105723DOI Listing
June 2021

circSAMD4A participates in the apoptosis and autophagy of dopaminergic neurons via the miR‑29c‑3p‑mediated AMPK/mTOR pathway in Parkinson's disease.

Mol Med Rep 2021 Jul 3;24(1). Epub 2021 Jun 3.

Department of Neurology, Ningbo No. 6 Hospital, Ningbo, Zhejiang 315040, P.R. China.

Parkinson's disease (PD) can lead to movement injury and cognitive dysfunction. Although advances have been made in attenuating PD, the effect of inhibiting the development of PD remains disappointing. Therefore, the present study aimed at investigating the etiology of Parkinson's disease and developing an alternative therapeutic strategy for patients with PD. A PD mouse model was established using an intraperitoneal injection of 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine hydrochloride (MPTP‑HCl; 30 mg/kg/day for 5 days), and a PD cellular model was established by treating SH‑SY5Y cells with different concentrations of 1‑methyl‑4‑phenylpyridinium (MPP) for 24 h. The expression levels of circular RNA sterile α motif domain containing 4A (circSAMD4A) and microRNA (miR)‑29c‑3p in both midbrain tissues and SH‑SY5Y cells were detected via reverse transcription‑quantitative PCR. The interaction between circSAMD4A and miR‑29c‑3p was verified using a dual‑luciferase reporter experiment. Apoptosis‑, autophagy‑ and 5'AMP‑activated protein kinase (AMPK)/mTOR cascade‑associated proteins in midbrain tissues and SH‑SY5Y cells were detected using western blotting. Furthermore, TUNEL staining and flow cytometry were used to analyze cell apoptosis. It was found that circSAMD4A was upregulated, while miR‑29c‑3p was downregulated in both PD animal and cellular models. Moreover, circSAMD4A directly targeted and negatively regulated miR‑29c‑3p. Further studies identified that circSAMD4A knockdown inhibited MPTP‑ or MPP‑induced apoptosis and autophagy; however, these effects were abolished by an miR‑29c‑3p inhibitor. In addition, circSAMD4A knockdown repressed phosphorylated‑AMPK expression and increased mTOR expression in MPTP‑ or MPP‑induced PD models, the effects of which were reversed by a miR‑29c‑3p inhibitor. Collectively, these results suggested that circSAMD4A participated in the apoptosis and autophagy of dopaminergic neurons by modulating the AMPK/mTOR cascade via miR‑29c‑3p in PD.
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http://dx.doi.org/10.3892/mmr.2021.12179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170871PMC
July 2021

Reciprocal adaptation of rice and Xanthomonas oryzae pv. oryzae: cross-species two-dimensional GWAS reveals the underlying genetics.

Plant Cell 2021 Jun 2. Epub 2021 Jun 2.

Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, 12 South Zhong-Guan-Cun Street, Haidian District, Beijing 100081, China.

A one-/two-dimensional genome-wide association study (GWAS) strategy was adopted to investigate the genetic systems underlying the reciprocal adaptation of rice (Oryza sativa) and its bacterial pathogen, Xanthomonas oryzae pv. oryzae (Xoo) using the whole-genome sequencing and large-scale phenotyping data of 701 rice accessions and 23 diverse Xoo strains. Forty-seven Xoo virulence-related genes and 318 rice quantitative resistance genes (QR-genes) mainly located in 41 genomic regions, and genome-wide interactions between the detected virulence-related genes and QR-genes were identified, including well-known resistance genes/virulence genes plus many previously uncharacterized ones. The relationship between rice and Xoo was characterized by strong differentiation among Xoo races corresponding to the subspecific differentiation of rice, by strong shifts towards increased resistance/virulence of rice/Xoo populations and by rich genetic diversity at the detected rice QR-genes and Xoo virulence genes, and by genome-wide interactions between many rice QR-genes and Xoo virulence genes in a multiple-to-multiple manner, presumably resulting either from direct protein-protein interactions or from genetic epistasis. The observed complex genetic interaction system between rice and Xoo likely exists in other crop-pathogen systems that would maintain high levels of diversity at their QR-loci/virulence-loci, resulting in dynamic coevolutionary consequences during their reciprocal adaptation.
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http://dx.doi.org/10.1093/plcell/koab146DOI Listing
June 2021

Recent Advances on Early Detection of Heat Strain in Dairy Cows Using Animal-Based Indicators: A Review.

Animals (Basel) 2021 Apr 1;11(4). Epub 2021 Apr 1.

AgroBioChem/TERRA, Precision Livestock and Nutrition Unit, Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, Belgium.

In pursuit of precision livestock farming, the real-time measurement for heat strain-related data has been more and more valued. Efforts have been made recently to use more sensitive physiological indicators with the hope to better inform decision-making in heat abatement in dairy farms. To get an insight into the early detection of heat strain in dairy cows, the present review focuses on the recent efforts developing early detection methods of heat strain in dairy cows based on body temperatures and respiratory dynamics. For every candidate animal-based indicator, state-of-the-art measurement methods and existing thresholds were summarized. Body surface temperature and respiration rate were concluded to be the best early indicators of heat strain due to their high feasibility of measurement and sensitivity to heat stress. Future studies should customize heat strain thresholds according to different internal and external factors that have an impact on the sensitivity to heat stress. Wearable devices are most promising to achieve real-time measurement in practical dairy farms. Combined with internet of things technologies, a comprehensive strategy based on both animal- and environment-based indicators is expected to increase the precision of early detection of heat strain in dairy cows.
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http://dx.doi.org/10.3390/ani11040980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066310PMC
April 2021

Efficacy and safety of duloxetine for postoperative pain after total knee arthroplasty in centrally sensitized patients: study protocol for a randomized controlled trial.

BMC Musculoskelet Disord 2021 Mar 30;22(1):316. Epub 2021 Mar 30.

Department of Orthopaedic Surgery, Ningbo No.6 Hospital, 1059 Zhongshan East Road, Ningbo, 315040,, Zhejiang, China.

Background: Postoperative residual knee pain after total knee arthroplasty (TKA) is a significant factor that contributes to patient dissatisfaction. Patients with preoperative central sensitization (CS) may be more susceptible to unexplained chronic pain after TKA, and duloxetine has been reported to be effective in post-TKA pain control in patients with CS. However, there remains limited evidence to support this off-label use in routine clinical practice. Hence, we designed this randomized, placebo-controlled, triple-blind clinical trial to evaluate the effects of preoperative screening and targeted duloxetine treatment of CS on postoperative residual pain compared with the care-as-usual control group.

Methods: This randomized controlled trial includes patients with knee osteoarthritis on a waiting list for primary unilateral TKA. Patients with preoperative CS will be randomly allocated to the perioperative duloxetine treatment group (duloxetine group) or the care-as-usual control group (placebo group). Patients in the duloxetine group will receive a half-dose of preemptive duloxetine (30 mg/day) for a week before surgery and a full-dose of duloxetine (60 mg/day) for six weeks after surgery. The primary outcome is the intensity of residual pain at six months after TKA, including the visual analogue scale, 11-point numeric rating scale, the sensory dimension of the brief pain inventory, and the pain subscale of the Knee injury and Osteoarthritis Outcome Score. The secondary outcome measures will include the pain and function related outcomes. All of the patients will be followed up at one, three, and six months after surgery. All adverse events will be recorded and immediately reported to the primary investigator and ethics committee to decide if the patient needs to drop out from the trial.

Discussion: This clinical trial will convey the latest evidence of the efficacy and safety of the application of duloxetine in postoperative pain control in CS patients who are scheduled for TKA. The study results will be disseminated at national and international conferences and published in peer-reviewed journals.

Trial Registration: Chinese Clinical Trial Registry ( http://www.chictr.org.cn ) registration number: ChiCTR2000031674 . Registered 07 April 2020.
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http://dx.doi.org/10.1186/s12891-021-04168-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008334PMC
March 2021

The effects of three different contrast agents (Gd-BOPTA, Gd-DTPA, and Gd-DOTA) on brachial plexus magnetic resonance imaging.

Ann Transl Med 2021 Feb;9(4):344

Medical School of Chinese People's Liberation Army, Beijing, China.

Background: MRI is very important for guiding the diagnosis and treatment of brachial plexus diseases. The most used type of MRI brachial plexus imaging is the 3D Short Term Inversion Recovery (STIR) sequence with contrast agent. This study aimed to investigate the effect of three contrast agents; gadobenate dimeglumine (Gd-BOPTA), gadopentetate dimeglumine (Gd-DTPA), and Gadoteric Acid Meglumine (Gd-DOTA) on brachial plexus magnetic resonance imaging (MRI).

Methods: We recruited 60 patients with suspected brachial plexus injury randomly into three groups. MRI images were obtained from each patient. Prior to scanning, the first group was injected with GD-BOPTA, the second group with Gd-DTPA, and the third with Gd-DOTA. The amount of contrast agent was 0.1 mmol/kg according to the weight of each patient, the injection rate was 1.5 mL/s, and 20 mL saline was injected at the same rate with a high-pressure injector. Immediately after the injection of contrast agent and saline, a 3D Sampling perfection with application optimized contrasts using different flip angle evolutions (SPACE) STIR sequence was used for scanning. The Signal Intensity (SI) and Standard Deviation (SD) of Maximal intensity projection (MIP) images for regions outside the anatomy (ROI background) with area of 17 mm on both sides of the C6 peripheral nerves (ROI nerve), and tissue adjacent to the peripheral nerves (ROI tissue) were obtained. Signal to noise ratio (SNR) and contrast to noise ratio (CNR) were then calculated.

Results: The SNR was 40.66±25.27, 34.65±14.86, and 44.63±30.79 for Gd-BOPTA, Gd-DTPA, and Gd-DOTA, respectively and the CNR was 20.24±15.17, 16.07±7.50, and 20.84±15.53 for Gd-BOPTA, Gd-DTPA, and Gd-DOTA, respectively. In addition, there was no statistical difference in the SNR or CNR of brachial plexus nerves using the three contrast agents to enhance the 3D SPACE sequence χ=1.877, P=0.391>0.05 and χ=1.717, P=0.424, respectively.

Conclusions: There were no significant differences in the efficacy of three contrast agents in imaging the brachial plexus.
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http://dx.doi.org/10.21037/atm-21-348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944290PMC
February 2021

Comparative transcriptome and metabolome profiling reveal molecular mechanisms underlying OsDRAP1-mediated salt tolerance in rice.

Sci Rep 2021 Mar 4;11(1):5166. Epub 2021 Mar 4.

Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, South Zhong-Guan-Cun Street 12#, Beijing, 100081, China.

Integration of transcriptomics and metabolomics data can provide detailed information for better understanding the molecular mechanisms underlying salt tolerance in rice. In the present study, we report a comprehensive analysis of the transcriptome and metabolome of rice overexpressing the OsDRAP1 gene, which encodes an ERF transcription factor and was previously identified to be conferring drought tolerance. Phenotypic analysis showed that OsDRAP1 overexpression (OE) improved salt tolerance by increasing the survival rate under salt stress. OsDRAP1 affected the physiological indices such as superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) to enhance redox homeostasis and membrane stability in response to salt stress. Higher basal expression of OsDRAP1 resulted in differential expression of genes that potentially function in intrinsic salt tolerance. A core set of genes with distinct functions in transcriptional regulation, organelle gene expression and ion transport were substantially up-regulated in the OE line in response to salt stress, implying their important role in OsDRAP1-mediated salt tolerance. Correspondingly, metabolome profiling detected a number of differentially metabolites in the OE line relative to the wild type under salt stress. These metabolites, including amino acids (proline, valine), organic acids (glyceric acid, phosphoenolpyruvic acid and ascorbic acid) and many secondary metabolites, accumulated to higher levels in the OE line, demonstrating their role in salt tolerance. Integration of transcriptome and metabolome analysis highlights the crucial role of amino acids and carbohydrate metabolism pathways in OsDRAP1-mediated salt tolerance.
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http://dx.doi.org/10.1038/s41598-021-84638-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933422PMC
March 2021

Noninvasive Assessment of O(6)-Methylguanine-DNA Methyltransferase Promoter Methylation Status in World Health Organization Grade II-IV Glioma Using Histogram Analysis of Inflow-Based Vascular-Space-Occupancy Combined with Structural Magnetic Resonance Imaging.

J Magn Reson Imaging 2021 07 16;54(1):227-236. Epub 2021 Feb 16.

Department of Medical Imaging, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: O(6)-methylguanine-DNA methyltransferase (MGMT) promoter methylation is an important prognostic factor for gliomas and is associated with tumor angiogenesis. Arteriolar cerebral blood volume (CBVa) obtained from inflow-based vascular-space-occupancy (iVASO) magnetic resonance imaging (MRI) is assumed to be an indicator of tumor microvasculature. Its preoperative predictive ability for MGMT promoter methylation remains unclear.

Purpose: To investigate the role of iVASO-CBVa histogram features in determining MGMT promoter methylation status of grade II-IV gliomas.

Study Type: Retrospective SUBJECTS: Forty-six patients consisting of 20 MGMT methylated and 26 unmethylated gliomas.

Field Strength/sequence: 3.0 T magnetic resonance images containing iVASO MRI, T -weighted image (T WI), T -weighted image, T -weighted fluid attenuated inversion recovery image images, and enhanced T WI.

Assessment: Sixteen structural imaging features were visually evaluated on structural MRI and 14 CBVa histogram features were extracted from iVASO-CBVa maps.

Statistical Tests: Imaging features were screened and ranked using Fisher's exact test, Mann-Whitney U-test, and randomforest algorithm. Features with higher importance were selected to develop logistic regression models to determine MGMT methylation status. Receiver operating characteristics (ROC) curve with the area under the curve (AUC) and leave-one-out cross-validation (LOOCV) were used to assess effectiveness and stability.

Results: The top two CBVa histogram features were root mean squared (RMS) and variance. The top two structural imaging features were contrast-enhancing component of the tumor (CET) location and tumor location. Both the CBVa model of RMS and variance (ROC, AUC = 0.867; LOOCV, AUC = 0.819) and the model of structural features (ROC, AUC = 0.882; LOOCV, AUC = 0.802) accurately identified MGMT methylation. The fusion model of CBVa RMS and CET location improved diagnostic performance (ROC, AUC = 0.931; LOOCV, AUC =0.906). DATA CONCLUSION: iVASO-CBVa has potential in evaluating MGMT methylation status in grade II-IV gliomas.

Level Of Evidence: 4 TECHNICAL EFFICACY: Stage 2.
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http://dx.doi.org/10.1002/jmri.27514DOI Listing
July 2021

The landscape of gene-CDS-haplotype diversity in rice: Properties, population organization, footprints of domestication and breeding, and implications for genetic improvement.

Mol Plant 2021 May 10;14(5):787-804. Epub 2021 Feb 10.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; College of Agronomy, Anhui Agricultural University, Hefei, Anhui, 230036, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong, 518120, China. Electronic address:

Polymorphisms within gene coding regions represent the most important part of the overall genetic diversity of rice. We characterized the gene-coding sequence-haplotype (gcHap) diversity of 45 963 rice genes in 3010 rice accessions. With an average of 226 ± 390 gcHaps per gene in rice populations, rice genes could be classified into three main categories: 12 865 conserved genes, 10 254 subspecific differentiating genes, and 22 844 remaining genes. We found that 39 218 rice genes carry >255 179 major gcHaps of potential functional importance. Most (87.5%) of the detected gcHaps were specific to subspecies or populations. The inferred proto-ancestors of local landrace populations reconstructed from conserved predominant (ancient) gcHaps correlated strongly with wild rice accessions from the same geographic regions, supporting a multiorigin (domestication) model of Oryza sativa. Past breeding efforts generally increased the gcHap diversity of modern varieties and caused significant frequency shifts in predominant gcHaps of 14 266 genes due to independent selection in the two subspecies. Low frequencies of "favorable" gcHaps at most known genes related to rice yield in modern varieties suggest huge potential for rice improvement by mining and pyramiding of favorable gcHaps. The gcHap data were demonstrated to have greater power than SNPs for the detection of causal genes that affect complex traits. The rice gcHap diversity dataset generated in this study would facilitate rice basic research and improvement in the future.
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http://dx.doi.org/10.1016/j.molp.2021.02.003DOI Listing
May 2021

Application of machine learning methods to pathogen safety evaluation in biological manufacturing processes.

Biotechnol Prog 2021 Feb 1:e3135. Epub 2021 Feb 1.

Pharma Biotech - Lab Automation & Data Management, Bayer Pharmaceuticals, Berkeley, California, USA.

The production of recombinant therapeutic proteins from animal or human cell lines entails the risk of endogenous viral contamination from cell substrates and adventitious agents from raw materials and environment. One of the approaches to control such potential viral contamination is to ensure the manufacturing process can adequately clear the potential viral contaminants. Viral clearance for production of human monoclonal antibodies is achieved by dedicated unit operations, such as low pH inactivation, viral filtration, and chromatographic separation. The process development of each viral clearance step for a new antibody production requires significant effort and resources invested in wet laboratory experiments for process characterization studies. Machine learning methods have the potential to help streamline the development and optimization of viral clearance unit operations for new therapeutic antibodies. The current work focuses on evaluating the usefulness of machine learning methods for process understanding and predictive modeling for viral clearance via a case study on low pH viral inactivation.
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http://dx.doi.org/10.1002/btpr.3135DOI Listing
February 2021

Integrated Analysis of the Transcriptome and Metabolome Revealed the Molecular Mechanisms Underlying the Enhanced Salt Tolerance of Rice Due to the Application of Exogenous Melatonin.

Front Plant Sci 2020 14;11:618680. Epub 2021 Jan 14.

Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, China.

High salinity is one of the major abiotic stresses limiting rice production. Melatonin has been implicated in the salt tolerance of rice. However, the molecular basis of melatonin-mediated salt tolerance in rice remains unclear. In the present study, we performed an integrated transcriptome and metabolome profiling of rice seedlings treated with salt, melatonin, or salt + melatonin. The application of exogenous melatonin increased the salt tolerance of rice plants by decreasing the sodium content to maintain Na/K homeostasis, alleviating membrane lipid oxidation, and enhancing chlorophyll contention. A comparative transcriptome analysis revealed that complex molecular pathways contribute to melatonin-mediated salt tolerance. More specifically, the AP2/EREBP-HB-WRKY transcriptional cascade and phytohormone (e.g., auxin and abscisic acid) signaling pathways were activated by an exogenous melatonin treatment. On the basis of metabolome profiles, 64 metabolites, such as amino acids, organic acids, nucleotides, and secondary metabolites, were identified with increased abundances only in plants treated with salt + melatonin. Several of these metabolites including endogenous melatonin and its intermediates (5-hydroxy-L-tryptophan, -acetyl- -formyl-5-methoxykynuramine), gallic acid, diosmetin, and cyanidin 3--galactoside had antioxidant functions, suggesting melatonin activates multiple antioxidant pathways to alleviate the detrimental effects of salt stress. Combined transcriptome and metabolome analyses revealed a few gene-metabolite networks related to various pathways, including linoleic acid metabolism and amino acid metabolism that are important for melatonin-mediated salt tolerance. The data presented herein may be useful for further elucidating the multiple regulatory roles of melatonin in plant responses to abiotic stresses.
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http://dx.doi.org/10.3389/fpls.2020.618680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840565PMC
January 2021

Experimental Characterization of Viscoelastic Behaviors of Nano-TiO/CaCO Modified Asphalt and Asphalt Mixture.

Nanomaterials (Basel) 2021 Jan 4;11(1). Epub 2021 Jan 4.

College of Transportation, Jilin University, Changchun 130025, China.

The purpose of this paper is to promote the application of nano-TiO/CaCO in bituminous materials and present an experimental characterization of viscoelastic behaviors of bitumen and bituminous mixture modified by nano-TiO/CaCO. In this work, a series of viscoelastic behavior characterization tests were conducted, including dynamic shear rheometer (DSR) test for bitumen, uniaxial static compression creep test and dynamic modulus test for bituminous mixture. Moreover, various viscoelastic models with clear physical meanings were used to evaluate the influence of nano-TiO/CaCO on the macroscopic performance of bitumen and bituminous mixture. The results show that bitumen and its mixtures are time-temperature dependent. The Christensen-Anderson-Marasteanu (CAM) model of frequency sweep based on DSR test indicated that adding nano-TiO/CaCO can effectively capture the sensitivity of temperature. In addition, the incorporation of nano-TiO/CaCO in bituminous mixture can significantly enhance the high-temperature anti-rutting, and slightly improve the low-temperature anti-cracking as well. At the same time, the modified Burgers model can accurately describe the viscoelastic behavior of bituminous mixtures in the first two creep stages, reflecting the consolidation effect of bituminous mixture. Also, the generalized Sigmoidal model can accurately grasp the characteristics of the relationship between dynamic modulus and reduced frequency and achieve good prediction effects in a wider frequency range.
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http://dx.doi.org/10.3390/nano11010106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824758PMC
January 2021

Screening for and combining serum intestinal barrier-related biomarkers to predict the disease severity of AECOPD.

Ann Palliat Med 2021 Feb 17;10(2):1548-1559. Epub 2020 Nov 17.

Department of General Surgery, Xinqiao Hospital, Army Military Medical University, Chongqing, China.

Background: In the patients with AECOPD, the gut displays ischemia, anoxia and oxidative stress, which lead to the intestinal barrier failure. Therefore, it is desirable to screen for effective intestinal barrier-related biomarkers to monitor the disease severity.

Methods: We conducted a prospective observational study in 40 patients with AECOPD and 10 patients with stable COPD. The serum levels of I-FABP, citrulline, D-Lactate, DAO, and α-GST, as well as the APACHE II scores were recorded. Person correlation analysis, logistic regression models and receiver operating characteristic (ROC) curve analyses were used in our study.

Results: Patients with AECOPD had significantly higher levels of I-FABP, D-Lactate, and DAO than did those with stable COPD. However, the serum citrulline level was significantly decreased in the patients with stable COPD than in those with AECOPD and the serum α-GST was not significantly changed. Additionally, we observed that there was a higher levels of I-FABP, D-Lactate, and DAO and a lower level of citrulline in patients with severe COPD than in patients with nonsevere COPD [APACHE II (nonsevere COPD) <20; APACHE II (severe COPD) ≥20]. Correlation analysis showed that I-FABP and D-Lactate had a significantly positive correlations with the APACHE II score, and citrulline had a significantly negative correlations with the APACHE II score. Following, treatment, the levels of I-FABP and D-lactate were decreased and the level of citrulline was increased. Moreover, we screened out the citrulline and DAO, which independently affected the diagnosis of severe COPD by stepwise logistics regression analysis. Additionally, we found that the combination of serum citrulline and DAO can more effectively diagnose the severe COPD than any single biomarker can, which may be a supportive and convenient method that can be used clinically.

Conclusions: Serum I-FABP, citrulline and D-Lactate could be used to assess the disease severity. Citrulline and DAO can diagnose the severe COPD and the combination is more effective.
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http://dx.doi.org/10.21037/apm-20-1060DOI Listing
February 2021

DNA replication and sister chromatid cohesion 1 promotes breast carcinoma progression by modulating the Wnt/β-catenin signaling and p53 protein.

J Biosci 2020 ;45

Department of Breast and Thyroid Surgery, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong, China.

The objective of this study is to assess the prognostic and functional role of DSCC1 in breast carcinoma, as well as the potential mechanism. Based upon the TCGA data, the expression pattern and prognostic value of DSCC1 in breast carcinoma was evaluated. The mRNA and protein levels of molecules were determined using qRT-PCR and Western blot. In vitro functional role of DSCC1 in tumor cells was determined using cell counting kit 8, clone formation, and Transwell assays. Gene set enrichment analysis (GSEA) was conducted to determine DSCC1 related gene sets, which are further confirmed by Western blot. The results showed that DSCC1 is overexpressed in breast carcinoma tissues and its high expression was linked to shorter overall survival. Overexpression of DSCC1 facilitated the proliferation, invasion and migration of breast carcinoma cells, while knockdown of DSCC1 showed opposite outcomes. GSEA showed that high DSCC1 expression had a positive correlation with p53, and Wnt signaling-related molecules. Western blot showed that silencing DSCC1 increased the levels of p53 and p-β-catenin, whereas decreased p-GSK-3β and cyclin D1 expression. These observations illustrate that DSCC1 emerges a well value on the diagnosis and prognosis of breast carcinoma, and facilitates the progression of breast carcinoma partly by activating Wnt/b-catenin signaling and inhibiting p53.
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January 2020

Evaluating postoperative anal fistula prognosis by diffusion-weighted MRI.

Eur J Radiol 2020 Nov 21;132:109294. Epub 2020 Sep 21.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China; Department of Radiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Purpose: The purpose of this study was to explore whether preoperative diffusion-weighted magnetic resonance imaging (DW-MRI) can be used to evaluate the prognosis of anal fistula and identify the influence factors of postoperative recurrence.

Methods: This is a retrospective study of 117 patients with anal fistula who have undergone preoperative DW-MRI and surgery. All patients were followed up by telephone or reexamination within 2 years after surgery. Of the 117 patients, 35 were excluded due to loss of follow-up and only 82 were included in this study. MRI fistula imaging-related data were analyzed, and fistula severity was scored using criteria of both local extension of fistulas and active inflammation for a total maximum score of 22. The apparent diffusion coefficient (ADC) value of the fistula in patients with anal fistula during preoperative MRI examination was measured. According to whether anal fistula patients are accompanied by perianal abscess, they are divided into two groups, namely anal fistula group and anal fistula with abscess group. Based on whether patients with anal fistula recur after surgery, they were further divided into recurrent group and non-recurrent group.

Results: 82 patients with anal fistula were included in this analysis, 23 of them recurred and 59 were cured. Among patients with perianal abscess, the mean ADC value of the recurrent group was (1.19 ± 0.21)×10 mm/s, which is significantly lower than that of the non-recurrent group (1.36 ± 0.19)×10 mm/s. There were significant statistical differences in ADC values between the two groups (p = 0.03). Among patients with anal fistulas without abscesses, 15 patients recurred after surgery, with a mean ADC value of (1.45 ± 0.27) ×10 mm/s, and 33 patients didn't occur, with a mean ADC value of (1.44 ± 0.31)×10 mm/s. The ADC value of preoperative fistula in patients was negative significant correlation with MRI findings score (r= -0.332, P = 0.002). Risk factors for the recurrence after anal fistula surgery include the time interval between MRI and operation, multiple fistula tracks. Fatigue, excessive intake of spicy or greasy food and diarrhea may also be external risk factors for postoperative recurrence of patients with anal fistula.

Conclusions: DW-MRI has important application value for the prognosis evaluation of anal fistula. Complex type of anal fistula and improper lifestyle are the main risk factors affecting the recurrence after anal fistula surgery.
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http://dx.doi.org/10.1016/j.ejrad.2020.109294DOI Listing
November 2020

A Retrospective Study of Factors Influencing the Survival of Modified Meek Micrografting in Severe Burn Patients.

J Burn Care Res 2021 Mar;42(2):331-337

Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Modified Meek micrografting is a common method for treating severe burn patients. This study was to analyze the factors affecting the survival of modified Meek micrografting, thereby improving the survival rate of skin grafts. Eighty-three patients who underwent modified Meek micrografting were analyzed. According to the survival rate of skin graft after operation, the patients were divided into good skin survival group (GSSG, survival rate ≥ 70%, 47 cases) and poor skin survival group (PSSG, survival rate < 70%, 36 cases). The baseline data, surgical information, perioperative laboratory indicators, and prognosis of the patients were statistically analyzed. The univariate analysis and repeated measurement showed the burn severity, Meek skin graft area, duration of anesthesia, the postoperative sepsis shock, the mortality, the neutrophils percentage on the third day after surgery (NEU3), and the growth rate of neutrophils percentage from the first to third day after surgery (NEU3-1%) in the GSSG were significantly lower than those in the PSSG, whereas the perioperative average albumin levels and the perioperative average pre-albumin levels were higher. Receiver operating characteristic curve showed that the NEU3 had a good predictive value for the survival of skin slices. Maintaining perioperative albumin levels at a high level, controlling perioperative infection, and shortening the operation time as much as possible may improve the survival rate of modified Meek micrografting.
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http://dx.doi.org/10.1093/jbcr/iraa163DOI Listing
March 2021

Pretreatment structural and arterial spin labeling MRI is predictive for p53 mutation in high-grade gliomas.

Br J Radiol 2020 Nov 2;93(1115):20200661. Epub 2020 Sep 2.

Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, No. 107 Yanjiang Road West, Guangzhou, China.

Objectives: To determine the performance of pretreatment structural and arterial spin labelling (ASL) MRI in predicting p53 mutation in patients with high-grade gliomas (HGGs).

Methods: Pre-treatment structural and ASL MRI were performed in 57 patients with histologically confirmed HGGs and information of p53 status. Whole-lesion histogram analysis of cerebral blood flow (CBF) images of the enhancing tumour and the peritumoral oedema in the HGGs were performed. Visually AcceSAble Rembrandt Images features were used as qualitative analysis. The differences of ASL histogram parameters and Visually AcceSAble Rembrandt Images features between HGGs with or without p53 mutation were analyzed with post hoc correction for multiple comparisons. LASSO regression was performed to select the optimal features that could predict p53 mutation, followed by receiver operating characteristic analysis to determine the predictive efficacy.

Results: A total of 33 HGGs with p53 mutation and 24 without p53 mutation were included. HGGs with mutant p53 showed lower CBF and CBF of the enhancing tumour ( < 0.05) and higher prevalence of the qualitative MRI feature of enhancing tumour crossing midline (ETCM) ( < 0.05) as compared with HGGs with wild-type p53. LASSO regression showed that the CBF of the enhancing tumour and ETCM were predictive features for p53 mutation. CBF showed an acceptable performance in predicting p53 mutation (area under the curve = 0.721), when combined with the feature of ETCM, its predictive efficacy was significantly improved (area under the curve = 0.814, = 0.012).

Conclusion: An integrated pre-treatment structural and ASL MRI can help to predict p53 mutation in HGGs.
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http://dx.doi.org/10.1259/bjr.20200661DOI Listing
November 2020

Study on Viscoelastic Properties of Asphalt Mixtures Incorporating SBS Polymer and Basalt Fiber under Freeze-Thaw Cycles.

Polymers (Basel) 2020 Aug 11;12(8). Epub 2020 Aug 11.

College of Transportation, Jilin University, Changchun 130025, China.

This study aims to study the viscoelastic properties of asphalt mixtures incorporating styrene-butadiene-styrene (SBS) polymer and basalt fiber under freeze-thaw (F-T) cycles by using the static creep test. Asphalt mixture samples incorporating styrene-butadiene-styrene (SBS) polymer and basalt fiber were manufactured following the Superpave gyratory compaction (SGC) method and coring as well as sawing. After 0 to 21 F-T cycles processing, a uniaxial compression static creep test for the asphalt mixture specimens was performed to evaluate the influence of F-T cycles. The results indicated that the F-T cycles caused a larger creep deformation in the asphalt mixtures, which led to a decrease in the rut resistance of the asphalt mixtures incorporating SBS polymer and basalt fiber. Besides, the resistance to deformation decreased significantly in the early stage of F-T cycles. On the other hand, the viscoelastic parameters were analyzed to discuss the variation of viscoelastic characteristics. The relaxation time increased with F-T cycles, which will not be conducive to internal stress dissipation. Compared with lignin fiber, basalt fiber can improve the resistance to high-temperature deformation and the low-temperature crack resistance of asphalt mixtures under F-T cycles.
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http://dx.doi.org/10.3390/polym12081804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465384PMC
August 2020

Establishment of Complex Modulus Master Curves Based on Generalized Sigmoidal Model for Freeze-Thaw Resistance Evaluation of Basalt Fiber-Modified Asphalt Mixtures.

Polymers (Basel) 2020 Jul 29;12(8). Epub 2020 Jul 29.

College of Transportation, Jilin University, Changchun 130025, China.

This study aims to study the freeze-thaw (F-T) resistance of asphalt mixture incorporating styrene-butadiene-styrene (SBS) polymer and basalt fiber by using the established complex master curves of the generalized Sigmoidal model. Asphalt mixture samples incorporating styrene-butadiene-styrene (SBS) polymer and basalt fiber were manufactured following the Superpave gyratory compaction (SGC) method and coring as well as sawing. After 0-21 F-T cycles processing, a complex modulus test asphalt mixture specimen was performed to evaluate the influence of the F-T cycle. Besides, according to the time-temperature superposition principle, the master curves of a complex modulus were constructed to reflect the dynamic mechanical response in an extended range of reduced frequency at an arbitrary temperature. The results indicated that the elastic and viscous portions of asphalt mixture incorporating SBS and basalt fiber have decreased overall. It could be observed from the dynamic modulus ratio that the dynamic modulus ratios of specimens were more affected by the F-T cycle at low frequency or high temperature. Thus, in the process of asphalt pavement design and maintenance, attention should be paid to seasonal frozen asphalt pavement under low frequency and high temperature.
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http://dx.doi.org/10.3390/polym12081698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464322PMC
July 2020

Whole brain radiotherapy (WBRT) for leptomeningeal metastasis from NSCLC in the era of targeted therapy: a retrospective study.

Radiat Oncol 2020 Jul 31;15(1):185. Epub 2020 Jul 31.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, P. R. China.

Background And Purpose: Leptomeningeal metastasis (LM) is a rare but detrimental complication in patients with non-small cell lung cancer (NSCLC). Although whole brain radiotherapy (WBRT) is used to eliminating cancer cells or microscopic foci, it is becoming less favorable due to the concerns over neurocognitive toxicity. This study aimed to re-evaluate the role of WBRT in the setting of modern targeted therapy.

Materials And Methods: From December 2014 to March 2019, 80 NSCLC patients with cytologically and/or radiologically proven LM diagnosis were retrospectively analyzed.

Results: The median OS (mOS) after diagnosis of LM was 8.0 (95%CI: 4.4 to 11.6) months, and the one-year OS was 39.4%. The mOS for EGFR-mutated LM patients was 12.6 (3.0 to 22.2) months versus only 4.1 (2.8 to 5.4) for patients with wild-type EGFR (P < 0.001). Younger patients (< 53.5 yrs.) appeared to have a better OS than older patients (≥53.5 yrs.) (12.6 vs. 6.1, P = 0.041). No survival benefits were found in EGFR-mutated patients who received WBRT (P = 0.490). In contrast, mOS was significantly prolonged in wild-type EGFR patients with WBRT versus non-WBRT (mOS: 8.0 vs. 2.1, P = 0.002). Multivariate analysis indicated that WBRT (P = 0.025) and younger age (P = 0.048) were independent prognostic factors that predicted prolonged survival for wild-type EGFR LM patients from NSCLC.

Conclusion: Our study demonstrated that WBRT has clear survival advantages for patients with wild-type EGFR, and molecular biological stratification of LM patients for WBRT is highly recommended.
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http://dx.doi.org/10.1186/s13014-020-01627-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393872PMC
July 2020

Preliminary analysis of interaction of the fat fraction in the sacroiliac joint among sex, age, and body mass index in a normal Chinese population.

J Int Med Res 2020 Jul;48(7):300060520931281

Department of Radiology, The First Medical Centre, Chinese PLA General Hospital, Beijing, China.

Objective: Iterative decomposition of water and fat with echo asymmetry and least-squares estimation-iron quantification (IDEAL-IQ) is a noninvasive and objective method used to quantitatively measure fat content. Although this technique has been used in the entire abdomen, IDEAL-IQ findings in the sacroiliac joint (SIJ) have rarely been reported. This preclinical study was performed to quantify the amount of fat in the SIJ in healthy volunteers by IDEAL-IQ.

Methods: From April to November 2017, 60 healthy volunteers with low back pain were included in this retrospective study. The participants were allocated into groups by age (15-30, 31-50, and ≥51 years), sex (male and female), and body mass index (BMI) (<18.5, 18.5-23.9, and ≥24.0 kg/m). The iliac-side (Fi) and sacral-side (Fs) fat fractions were obtained in all groups. Two- and three-factor multivariate analyses were performed to analyze the effects of sex, age, and BMI on the Fi and Fs.

Results: The interaction among sex, age, and BMI had no statistically significant effect on the dependent variable. Both Fi and Fs were significantly influenced by age. Fs was significantly influenced by sex.

Conclusion: The IDEAL-IQ sequence can be used to quantitatively assess the SIJ fat content in healthy volunteers.
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http://dx.doi.org/10.1177/0300060520931281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391443PMC
July 2020

Master Curve Establishment and Complex Modulus Evaluation of SBS-Modified Asphalt Mixture Reinforced with Basalt Fiber Based on Generalized Sigmoidal Model.

Polymers (Basel) 2020 Jul 17;12(7). Epub 2020 Jul 17.

College of Transportation, Jilin University, Changchun 130025, China.

Basalt fiber has been proved to be a good modified material for asphalt mixture. The performance of basalt fiber modified asphalt mixture has been widely investigated by extensive researches. However, most studies focused on ordinary static load tests, and less attention was paid to the dynamic mechanical response of asphalt mixture incorporating with basalt fiber. This paper aims to establish the master curve of complex modulus of asphalt mixture incorporating of styrene-butadiene-styrene (SBS) polymer and basalt fiber using the generalized Sigmoidal model. Both loading frequency and temperature were investigated for dynamic mechanical response of asphalt mixture with basalt fiber. In addition, based on the time-temperature superposition principle, the master curves of complex modulus were constructed to reflect the dynamic mechanical response at an extended reduced frequency range at an arbitrary temperature. Results indicated that the generalized Sigmoidal model in this paper could better reflect the dynamic mechanical response accurately with correlation coefficients above 0.97, which is utilized to predict the dynamic mechanical performances accurately. Simultaneously, the modulus values exhibit an increasing trend with loading frequency and decrease versus temperature. However, the phase angle values showed different trends with frequency and temperature.
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http://dx.doi.org/10.3390/polym12071586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407966PMC
July 2020

Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice.

Rice (N Y) 2020 Jul 23;13(1):50. Epub 2020 Jul 23.

Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, China.

Background: High soil salinity can cause significant losses in rice productivity worldwide, mainly because salt inhibits plant growth and reduces grain yield. To cope with environmental changes, plants have evolved several adaptive mechanisms that involve the regulation of many stress-responsive genes.

Results: In this study, we identified OsSTAP1, which encodes an AP2/ERF-type transcription factor, was rapidly induced by ABA, ACC, salt, cold, and PEG treatments. OsSTAP1 is localized to the nucleus and acts as a transcriptional activator in plant cells. Compared with wild type, transgenic lines overexpressing OsSTAP1 exhibited increased tolerance to salt stress with higher SOD, POD, and CAT activities, and lower Na/K ratios in the shoots. In addition, many other stress-responsive genes, including other ERF- and peroxidase-encoding genes, were upregulated in the OsSTAP1-overexpression lines.

Conclusion: This study suggests that OsSTAP1 functions as an AP2/ERF transcriptional activator, and plays a positive role in salt tolerance by decreasing the Na/K ratio and maintaining cellular redox homeostasis.
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http://dx.doi.org/10.1186/s12284-020-00405-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378131PMC
July 2020

Discriminative pattern of reduced cerebral blood flow in Parkinson's disease and Parkinsonism-Plus syndrome: an ASL-MRI study.

BMC Med Imaging 2020 07 13;20(1):78. Epub 2020 Jul 13.

Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China.

Background: Accurate identification of Parkinson's disease (PD) and Parkinsonism-Plus syndrome (PPS), especially in the early stage of the disease, is very important. The purpose of this study was to investigate the discriminative spatial pattern of cerebral blood flow (CBF) between patients with PD and PPS.

Methods: Arterial spin labeling (ASL) perfusion-weighted imaging was performed in 20 patients with PD (mean age 56.35 ± 7.56 years), 16 patients with PPS (mean age 59.62 ± 6.89 years), and 17 healthy controls (HCs, mean age 54.17 ± 6.58 years). Voxel-wise comparison of the CBF was performed among PD, PPS, and HC groups. The receiver operating characteristic (ROC) curve was used to evaluate the performance of CBF in discriminating between PD and PPS. The relationship between CBF and non-motor neuropsychological scores was assessed by correlation analysis.

Results: PD group showed a significantly decreased CBF in the right cerebelum_crus2, the left middle frontal gyrus (MFG), the triangle inferior frontal gyrus (IFG_Tri), the left frontal medial orbital gyrus (FG_Med_Orb) and the left caudate nucleus (CN) compared with the HC group (P < 0.05). Besides the above regions, the left supplementary motor area (SMA), the right thalamus had decreased CBF in the PPS group compared with the HC group (P < 0.05). PPS group had lower CBF value in the left MFG, the left IFG_Tri, the left CN, the left SMA, and the right thalamus compared with the PD group (P < 0.05). CBFs in left IFG_Tri, the left CN, the left SMA, and the right thalamus had moderate to high capacity in discriminating between PD and PPS patients (AUC 0.719-0.831). The CBF was positively correlated with the Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) scores in PD patients, while positively correlated with the MMSE, Hamilton Anxiety Scale (HAMA), Hamilton Depression Scale (HAMD) scores in PPS patients (P < 0.05).

Conclusion: PD and PPS patients have certain discriminative patterns of reduced CBFs, which can be used as a surrogate marker for differential diagnosis.
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http://dx.doi.org/10.1186/s12880-020-00479-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359235PMC
July 2020

Altered White Matter Structural Network in Frontal and Temporal Lobe Epilepsy: A Graph-Theoretical Study.

Front Neurol 2020 17;11:561. Epub 2020 Jun 17.

Department of Radiology, First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Temporal lobe epilepsy (TLE) and frontal lobe epilepsy (FLE) are the largest subgroup of partial epilepsy, and focal cortical dysplasias (FCDs) are highly epileptogenic brain lesions and are a frequent cause for antiepileptic drug (AED)-resistant focal epilepsies that mostly occur in the temporal and frontal lobes. We performed a graph-theoretical study based on the diffusion tensor imaging (DTI) data of patients with FLE or TLE caused by FCDs or lesions with high suspicion of FCDs and evaluated their cognitive function by the Chinese version of the Montreal Cognitive Assessment-Basic (MoCA-BC). The construction of the white matter structural network and graph-theoretical analysis was performed by Pipeline for Analysing Brain Diffusion Images (PANDA) and Graph-theoretical Network Analysis (GRETNA). We used the nonparametric analysis of covariance to compare the differences in diffusion metrics, network attributes and nodal attributes among FLE, TLE, and healthy control (HC) groups and then performed pairwise comparisons. Nonparametric Spearman partial correlation analysis was performed to analyse the correlation of network attributes with the age of onset, duration of disease, and MoCA-BC scores in patients with FLE and TLE. The results showed that the white matter structural network in patients with FLE and TLE was impaired in a more extensive set of regions than the FCD location. The similarities in white matter alterations between FLE and TLE suggested that their epileptogenic network might affect the fronto-temporal white matter tracts and thalamo-occipital connections, which might be responsible for the overlapping cognitive deficits in FLE and TLE. The white matter impairments in patients with FLE were more severe than those in patients with TLE, which might be explained by more affected nodes in the areas of DMN in patients with FLE.
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http://dx.doi.org/10.3389/fneur.2020.00561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311567PMC
June 2020

Near-infrared emission of CaAlGaO:Cr,Ln (Ln = Yb, Nd, and Er) via energy transfer for c-Si solar cells.

Dalton Trans 2020 Jul 18;49(25):8791-8798. Epub 2020 Jun 18.

Institute of Petrochemical Technology, Jilin Institute of Chemical Technology, Jilin 132022, China.

A series of CaAlGaO:Cr,Ln (Ln = Yb, Nd, and Er) novel near-infrared (NIR) emitting phosphors were prepared via a high-temperature solid-state reaction. The luminescence spectra and decay lifetimes of these samples were measured to prove the energy transfer process from the Cr to Ln ions. The CaAlGaO:Cr,Ln (Ln = Yb, Nd, and Er) phosphors can efficiently convert the short-wavelength sunlight in the near-ultraviolet and visible (UV-Vis) regions into near-infrared emission, which matches the higher sensitivity region of the c-Si solar cells. The maximal energy transfer efficiencies of CaAlGaO:Cr,Ln (Ln = Yb, Nd, and Er) were 25.84%, 73.90%, and 29.46%, respectively. Our results show that the CaAlGaO:Cr,Ln (Ln = Yb, Nd, and Er) phosphors have potential applications as luminescent downshifting convertors for enhancing the photoelectric conversion efficiency of the c-Si solar cells.
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http://dx.doi.org/10.1039/d0dt01623cDOI Listing
July 2020
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