Publications by authors named "Wenrui Li"

50 Publications

Idiopathic ovarian vein thrombosis causing pulmonary embolism: case report and literature review.

J Int Med Res 2021 Jun;49(6):3000605211010649

Department of Vascular Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Ovarian vein thrombosis (OVT) is a rare medical disorder, which is most often found in the immediate postpartum period. OVT is rarely considered idiopathic. We report a case of idiopathic OVT with pulmonary embolism in a 33-year-old woman who presented with abdominal pain. Computed tomography and postoperative pathology confirmed the diagnosis of idiopathic OVT. To date, only 12 cases of idiopathic OVT have been reported. In this case report, we present a summary of these cases and a review of literature regarding management of idiopathic OVT.
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http://dx.doi.org/10.1177/03000605211010649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258764PMC
June 2021

Assessment of a COVID-19 Control Plan on an Urban University Campus During a Second Wave of the Pandemic.

JAMA Netw Open 2021 06 1;4(6):e2116425. Epub 2021 Jun 1.

College of Engineering, Boston University, Boston, Massachusetts.

Importance: The COVID-19 pandemic has severely disrupted US educational institutions. Given potential adverse financial and psychosocial effects of campus closures, many institutions developed strategies to reopen campuses in the fall 2020 semester despite the ongoing threat of COVID-19. However, many institutions opted to have limited campus reopening to minimize potential risk of spread of SARS-CoV-2.

Objective: To analyze how Boston University (BU) fully reopened its campus in the fall of 2020 and controlled COVID-19 transmission despite worsening transmission in Boston, Massachusetts.

Design, Setting, And Participants: This multifaceted intervention case series was conducted at a large urban university campus in Boston, Massachusetts, during the fall 2020 semester. The BU response included a high-throughput SARS-CoV-2 polymerase chain reaction testing facility with capacity to deliver results in less than 24 hours; routine asymptomatic screening for COVID-19; daily health attestations; adherence monitoring and feedback; robust contact tracing, quarantine, and isolation in on-campus facilities; face mask use; enhanced hand hygiene; social distancing recommendations; dedensification of classrooms and public places; and enhancement of all building air systems. Data were analyzed from December 20, 2020, to January 31, 2021.

Main Outcomes And Measures: SARS-CoV-2 diagnosis confirmed by reverse transcription-polymerase chain reaction of anterior nares specimens and sources of transmission, as determined through contact tracing.

Results: Between August and December 2020, BU conducted more than 500 000 COVID-19 tests and identified 719 individuals with COVID-19, including 496 students (69.0%), 11 faculty (1.5%), and 212 staff (29.5%). Overall, 718 individuals, or 1.8% of the BU community, had test results positive for SARS-CoV-2. Of 837 close contacts traced, 86 individuals (10.3%) had test results positive for COVID-19. BU contact tracers identified a source of transmission for 370 individuals (51.5%), with 206 individuals (55.7%) identifying a non-BU source. Among 5 faculty and 84 staff with SARS-CoV-2 with a known source of infection, most reported a transmission source outside of BU (all 5 faculty members [100%] and 67 staff members [79.8%]). A BU source was identified by 108 of 183 undergraduate students with SARS-CoV-2 (59.0%) and 39 of 98 graduate students with SARS-CoV-2 (39.8%); notably, no transmission was traced to a classroom setting.

Conclusions And Relevance: In this case series of COVID-19 transmission, BU used a coordinated strategy of testing, contact tracing, isolation, and quarantine, with robust management and oversight, to control COVID-19 transmission in an urban university setting.
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http://dx.doi.org/10.1001/jamanetworkopen.2021.16425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233704PMC
June 2021

PUF60 of Japanese flounder is regulated by pol-miR-novel_395 and involved in pathogen infection, autophagy, and apoptosis.

Dev Comp Immunol 2021 Oct 16;123:104170. Epub 2021 Jun 16.

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Institute of Oceanology, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address:

MicroRNAs (miRNAs) are evolutionary conserved, non-coding small RNAs that have been shown to regulate diverse biological processes including immunity. In a previous study, a novel miRNA of Japanese flounder (Paralichthys olivaceus), pol-miR-novel_395, was found to be responsive in expression to the infection of the bacterial pathogen Edwardsiella tarda. In the present study, we examined the regulation and immune effect of pol-miR-novel_395 and its target gene. We found that pol-miR-novel_395 expression was regulated by E. tarda and megalocytivirus, and pol-miR-novel_395 targeted the gene of PUF60 (poly (U)-binding-splicing factor 60 kDa) of flounder (named PoPUF60). Constitutive expression of PoPUF60 occurred in relatively high levels in the heart and liver of flounder. Bacterial infection upregulated PoPUF60 expression, whereas viral infection downregulated PoPUF60 expression. Interference with PoPUF60 expression or overexpression of pol-miR-novel_395 in flounder cells strongly potentiated E. tarda infection. Consistently, in vivo knockdown of PoPUF60 enhanced bacterial dissemination in the tissues of flounder but blocked viral replication, whereas in vivo overexpression of PoPUF60 inhibited bacterial dissemination but facilitated viral replication. Additionally, pol-miR-novel_395 and PoPUF60 were involved in the process of autophagy and apoptosis. Collectively, these results indicated that PoPUF60 and pol-miR-novel_395 play an important role in pathogen infection, autophagy, and apoptosis.
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http://dx.doi.org/10.1016/j.dci.2021.104170DOI Listing
October 2021

Development of an evaluation indicator system for the rational use of proton pump inhibitors in pediatric intensive care units: An application of Delphi method.

Medicine (Baltimore) 2021 Jun;100(24):e26327

Department of Pharmacy.

Abstract: Proton pump inhibitors are frequently used to prevent gastrointestinal bleeding in critically ill patients. But there is little information in the instructions about the usages for children. It is important to monitor the appropriate use of proton pump inhibitors, especially in pediatrics. Therefore, we developed an evaluation indicator system for the rational use of proton pump inhibitors in pediatric intensive care units.First, a systematic review was conducted to developed the initial indicators. Then 2 rounds of Delphi surveys were conducted to collecting opinions from a panel of independent experts, and the indicator system was modified to form the final indicators according to the opinions. Finally, the analytic hierarchy procedure was used to determine the weight of each indicator.A total of 6 guidelines and 2 studies met the inclusion and exclusion criteria. Based on literature and discussion among experts, an initial indicator system including 4 first-rank indicators and 12 second-rank indicators was formed. After 2 rounds of Delphi surveys, 2 indicators were added, 5 indicators were deleted, and 1 indicator was revised, so the final indicator system contained a total of 13 indicators including 3 first-rank indicators (drug selection, drug usage and dosage, duration of drug therapy) and 10 second-rank indicators (the proportion of PPIs used in children, children under 1 year old, children who is using glucocorticoids, children with nonsteroidal anti-inflammatory drugs, children with gastroesophageal reflux disease, children with sepsis, children with ventilators in PICU; the strength of PPIs' use, the proportion of omeprazole in children using PPIs during the same period; the average days of PPIs used in children). By analyzing scores, all coefficients met the standard, indicating the indicators were scientific and credible.Through a two-round Delphi survey, 3 first-rank indicators and ten second-rank indicators were developed, which will help drug administrative departments to promote the rational use of PPIs for children in PICUs. What is more, our study can constitute a methodological reference for the development of other indicator systems.
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http://dx.doi.org/10.1097/MD.0000000000026327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213291PMC
June 2021

Multistructural Variational Reaction Kinetics of the Simplest Unsaturated Methyl Ester: H-Abstraction from Methyl Acrylate by H, OH, CH, and HO Radicals.

J Phys Chem A 2021 Jun 3;125(23):5103-5116. Epub 2021 Jun 3.

Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu, Sichuan 610031, China.

The H-abstraction reaction kinetics of methyl acrylate (MA) + H/OH/CH/HO radicals have been investigated theoretically in the present work. For these reactions, the reaction energies and barrier heights are first computed using several density functionals and compared to the coupled cluster CCSD(T)-F12/jun-cc-pVTZ benchmark calculations. The M062X/maug-cc-pVTZ method shows the best performance with the smallest mean unsigned deviation (MUD) of 0.42 kcal mol. Combined with the electronic structure calculations using the M062X/maug-cc-pVTZ method, the multistructural canonical variational transition-state theory (MS-CVT) with small-curvature tunneling (SCT) is employed to calculate the reaction rate constants at 500-2000 K. The variational effect is between 0.56 and 1.0, the multistructural torsional anharmonicity factor ranges from 0.004 to 4.57, and the tunneling coefficient is in the range of 0.5-4.70. Notably, given the existence of reactant complexes (RCs) between reactants and transition states for the reaction systems MA + OH/HO, we further compare the rate constants under the low-pressure limit (LPL) kinetic model, which treats the reaction as a single-step process and neglects RCs, and the pre-equilibrium model, which takes RCs into account in the reaction and treats the reaction as a two-step process. The rate constants calculated by these two models are similar within the combustion temperature range, and apparent differences occur at lower temperatures. In addition, we determine the branching ratios as a function of temperature and find that the methyl site (S3) abstractions by OH and H radicals are dominant in the low- and high-temperature ranges, respectively. Moreover, we update the kinetic model with the calculated H-abstraction rate constants to simulate the ignition delay times of MA. The simulations of the updated model are in good agreement with experimental results. The accurate reaction kinetics determined in this work are useful for the understanding and prediction of consumption branching fractions and ignition properties of the unsaturated methyl esters.
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http://dx.doi.org/10.1021/acs.jpca.1c01788DOI Listing
June 2021

Based on the whole genome clarified the evolution and expression process of fatty acid desaturase genes in three soybeans.

Int J Biol Macromol 2021 Jul 28;182:1966-1980. Epub 2021 May 28.

Institute of Soil and Water Conservation, Chinese Academy of Sciences & Ministry of Water Resource, Yangling 712100, China; University of the Chinese Academy of Sciences, Beijing 100049, China; Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address:

Soybean is an important oil crop cultivated worldwide. With the increasing global population crossed with growing challenging cultivation conditions, improving soybean breeding by selecting important traits is urgent needed. Genes coding for plant fatty acid desaturases (FADs) genes are major candidates for that, because they are involving in controlling fatty acid composition and holding membrane fluidity under abiotic stress. Here, 75 FADs were found in three soybean genomes, which were further classified into four sub-groups. Phylogenetic tree, gene structure, motif and promoter analysis showed that the FAD gene family was conserved in the three soybeans. In addition, the numbers of omega desaturase from Chinese cultivated varieties were significantly higher than those in Chinese wild soybean and ancient polyploid soybean, respectively. However, it was the opposite for the sphingolipid subfamily. These results indicated that each subfamily was subjected to different selection pressures during cultivation and domestication. As the extra genes of the subfamily were very close to other family members' positions on chromosomes, they should be produced by duplication. The cis-element analysis of FAD promoter sequences revealed that upstream sequences of FAD contained abundant light, hormone and abiotic stress responsive cis-elements, suggesting that the quality of soybean could be improved by regulating these stresses. Expression analysis of Chinese wild soybean under salt stress showed that GsDES1.1, GsDES1.2, GsFAD2.1 and GsSLD1 in leaves and GsSLD2, GsSLD5 and GsSLD6 in roots were not closely related to salt stress response. Therefore, we explored the significant role of conserved, duplicated and neofunctionalized FAD in the domestication of soybean, which contributes to the importance of soybean as a global oil crop.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.161DOI Listing
July 2021

Estimation of local time-varying reproduction numbers in noisy surveillance data.

medRxiv 2021 Apr 27. Epub 2021 Apr 27.

A valuable metric in understanding infectious disease local dynamics is the local time-varying reproduction number, i.e. the expected number of secondary local cases caused by each infected individual. Accurate estimation of this quantity requires distinguishing cases arising from local transmission from those imported from elsewhere. Realistically, we can expect identification of cases as local or imported to be imperfect. We study the propagation of such errors in estimation of the local time-varying reproduction number. In addition, we propose a Bayesian framework for estimation of the true local time-varying reproduction number when identification errors exist. And we illustrate the practical performance of our estimator through simulation studies and with outbreaks of COVID-19 in Hong Kong and Victoria, Australia.
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http://dx.doi.org/10.1101/2021.04.23.21255958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8095231PMC
April 2021

Chemical profiling and multicomponents quantitative analysis of Panzerina lanata by ultra-fast liquid chromatography with tandem mass spectrometry.

J Sep Sci 2021 Jul 25;44(13):2524-2535. Epub 2021 May 25.

School of Pharmacy, Key Laboratory of Ethnomedicine of Ministry of Education, Minzu University of China, Beijing, P. R. China.

Panzerina lanata is a Chinese medicine with the bioactivity of detumescence and detoxification. In this study, novel qualitative and quantitative methods were established by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry and ultra-high-performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry, respectively. As a result, 20 compounds were identified or tentatively characterized including flavonoids, organic acids, alkaloids, and lignans, five of which were identified for the first time based on the reference standards. The quantitative approach exhibited good linearity (R  > 0.995), precision (RSDs < 4.97%), stability (RSDs < 4.77%), and recovery (96.04-104.14%). Afterward, this method was implemented to determine 11 flavonoids in four batches of P. lanata. Among them, seven compounds were quantified for the first time. Narcissin was abundant in each batch of P. lanata (average of 10.890-14.230 mg/g) with the highest quantities. The results provide valuable information for quality evaluation.
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http://dx.doi.org/10.1002/jssc.202000944DOI Listing
July 2021

Lipid-Polymer Hybrid Nanoparticles Enhance the Potency of Ampicillin against in a Protozoa Infection Model.

ACS Infect Dis 2021 06 19;7(6):1607-1618. Epub 2021 Apr 19.

Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.

() biofilms are implicated in endocarditis, urinary tract infections, and biliary tract infections. Coupled with internalization into host cells, this opportunistic pathogen poses great challenges to conventional antibiotic therapy. The inability of ampicillin (Amp) to eradicate bacteria hidden in biofilms and intracellular niches greatly reduces its efficacy against complicated infections. To enhance the potency of Amp against different forms of infections, Amp was loaded into Lipid-Polymer hybrid Nanoparticles (LPNs), a highly efficient nano delivery platform consisting of a unique combination of DOTAP lipid shell and PLGA polymeric core. The antibacterial activity of these nanoparticles (Amp-LPNs) was investigated in a protozoa infection model, achieving a much higher multiplicity of infection (MOI) compared with studies using animal phagocytes. A significant reduction of total was observed in all groups receiving 250 μg/mL Amp-LPNs compared with groups receiving the same concentration of free Amp during three different interventions, simulating acute and chronic infections and prophylaxis. In early intervention, no viable was observed after 3 h LPNs treatment whereas free Amp did not clear after 24 h treatment. Amp-LPNs also greatly enhanced the antibacterial activity of Amp at late intervention and boosted the survival rate of protozoa approaching 400%, where no viable protozoa were identified in the free Amp groups at the 40 h postinfection treatment time point. Prophylactic effectiveness with Amp-LPNs at a concentration of 250 μg/mL was exhibited in both bacteria elimination and protozoa survival toward subsequent infections. Using protozoa as a surrogate model for animal phagocytes to study high MOI infections, this study suggests that LPN-formulated antibiotics hold the potential to significantly improve the therapeutic outcome in highly complicated bacterial infections.
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http://dx.doi.org/10.1021/acsinfecdis.0c00774DOI Listing
June 2021

Autistic traits and negative emotions in the general population during COVID-19: Mediating roles of the behavioural immune system and COVID-19 risk perception.

Psychiatry Res 2021 06 1;300:113918. Epub 2021 Apr 1.

Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Institute of Psychology and Behavioral Science, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Identifying the susceptibility factors of the emotional response to COVID-19 is highly significant for the psychological epidemic-crisis intervention, and autistic-related traits (ATs) is likely to be one of the candidate factors. The current study explored the relationships between ATs, emotional response to COVID-19, and the behavioural immune system (BIS) measured by trait pathogen avoidance and COVID-19 risk perception in the general population. The results showed that ATs predicted increased negative emotions directly and indirectly by enhancing the activation tendency of BIS and COVID-19 risk perception. The findings provide a candidate hypothesis for the reaction characteristics to pathogen threats in individuals with ASD and expand the understanding of individual differences in response to COVID-19.
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http://dx.doi.org/10.1016/j.psychres.2021.113918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015391PMC
June 2021

Emergence and characterization of a putative novel human adenovirus recombinant HAdV-C104 causing pneumonia in Southern China.

Virus Evol 2021 Jan 2;7(1):veab018. Epub 2021 Mar 2.

State Key Laboratory of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, P. R. China.

In 2017, a survey of the molecular epidemiology of human adenovirus (HAdV) infections in Southern China based on hexon and fiber genotype demonstrated that the most prevalent genotypes of HAdV were HAdV-3 ( = 62), HAdV-2 ( = 21), and HAdV-7 ( = 16). In addition, two patients were co-infected with two genotypes of HAdV. Interestingly, a novel human adenovirus C recombinant genotype strain was isolated from one of the pneumonia patients in this survey. Phylogenetic, recombination, and proteotyping analysis showed that this novel pathogen originated from the recombination of parental viruses harboring the HAdV-1 penton and hexon gene, and the HAdV-2 fiber gene. It was named 'P1H1F2' and was assigned as HAdV-C104 based on the nomenclature protocol of using three major capsid proteins for characterization. Subsequent experiments demonstrated that HAdV-C104 had comparable proliferation capacity to HAdV-1, HAdV-2, and another recombination genotype P1H2F2. In addition, the HAdV-C104 infected patient was diagnosed with pneumonia and recovered after antiviral therapy. This report strengthens the hypothesis of recombination as a major pathway for the molecular evolution of HAdV-C species.
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http://dx.doi.org/10.1093/ve/veab018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953211PMC
January 2021

Novel keratin 16 mutation in a Chinese family with focal palmoplantar keratoderma.

Int J Dermatol 2021 May 30;60(5):e187-e189. Epub 2020 Dec 30.

Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.

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http://dx.doi.org/10.1111/ijd.15386DOI Listing
May 2021

A targeted gene capture next-generation sequencing panel for genetic screening of newborns.

J Pak Med Assoc 2020 Oct;70(10):1789-1794

Department of Medical and Molecular Genetics, Dongguan Institute of Pediatrics, Dongguan, Guangdong, China.

Objective: The application of next-generation sequencing (NGS) will greatly promote the screening and diagnosis of genetic diseases. This study aimed to implement and validate a targeted NGS panel for genetic screening of over fifty types of genetic disorders in newborns.

Methods: A targeted gene panel consisting of 104 known genes related to genetic diseases with a target size of 347.8 kb was designed. Genes were selected through reference to databases including HGMD, OMIM, GeneReviews®, and Genetic Home Reference, and the latest peer-reviewed publications associated with the genetics of hereditary diseases.

Results: The average coverage for all targeted exons was 596X, and the mean targeted region coverage of 1X, 10X, 20X and 50X reads for each sample were 99.8%, 99.2%, 98.8%, and 95.3%, respectively. The panel showed 100% consistency in detecting 8 pathogenic insertion/deletion (indels) variants ranging from 1 to 16 bp in size and 20 pathogenic single-nucleotide variations (SNVs) across 32 samples previously confirmed by Sanger sequencing.

Conclusions: A dried blood spot (DBS)-based targeted NGS panel for efficient genetic screening of a wide variety of genetic diseases in newborns was developed and evaluated. Furthermore, our panel will contribute to providing accurate diagnosis for genetic disorders and will be helpful for gene therapy for specific diseases.
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http://dx.doi.org/10.5455/JPMA.28637DOI Listing
October 2020

Transcriptomic analysis reveals the GRAS family genes respond to gibberellin in Salvia miltiorrhiza hairy roots.

BMC Genomics 2020 Oct 27;21(1):727. Epub 2020 Oct 27.

Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, 712100, China.

Background: Salvia miltiorrhiza is one of the most important traditional Chinese medicinal plants with high medicinal value. Gibberellins are growth-promoting phytohormones that regulate numerous growth and developmental processes in plants. However, their role on the secondary metabolism regulation has not been investigated.

Results: In this study, we found that gibberellic acid (GA) can promote hairy roots growth and increase the contents of tanshinones and phenolic acids. Transcriptomic sequencing revealed that many genes involved in the secondary metabolism pathway were the GA-responsive. After further analysis of GA signaling pathway genes, which their expression profiles have significantly changed, it was found that the GRAS transcription factor family had a significant response to GA. We identified 35 SmGRAS genes in S. miltiorrhiza, which can be divided into 10 subfamilies. Thereafter, members of the same subfamily showed similar conserved motifs and gene structures, suggesting possible conserved functions.

Conclusions: Most SmGRAS genes were significantly responsive to GA, indicating that they may play an important role in the GA signaling pathway, also participating in the GA regulation of root growth and secondary metabolism in S. miltiorrhiza.
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http://dx.doi.org/10.1186/s12864-020-07119-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590604PMC
October 2020

Alterations of brain activity and functional connectivity in transition from acute to chronic tinnitus.

Hum Brain Mapp 2021 02 13;42(2):485-494. Epub 2020 Oct 13.

Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, China.

The objective of this study was to investigate alterations to brain activity and functional connectivity in patients with tinnitus, exploring neural features in the transition from acute to chronic phantom perception. Twenty-four patients with acute tinnitus, 23 patients with chronic tinnitus, and 32 healthy controls were recruited. High-density electroencephalography (EEG) was used to explore changes in brain areas and functional connectivity in different groups. When compared with healthy subjects, acute tinnitus patients had a significant reduction in superior frontal cortex activity across all frequency bands, whereas chronic tinnitus patients had a significant reduction in the superior frontal cortex at beta 3 and gamma frequency bands as well as a significant increase in the inferior frontal cortex at delta-band and superior temporal cortex at alpha 1 frequency band. When compared to the chronic tinnitus group, the acute tinnitus group activity was significantly increased in the middle frontal and parietal gyrus at the gamma-band. Functional connectivity analysis showed that the chronic tinnitus group had increased connections between the parahippocampus gyrus, posterior cingulate cortex, and precuneus when compared with the healthy group. Alterations of local brain activity and connections between the parahippocampus gyrus and other nonauditory areas appeared in the transition from acute to chronic tinnitus. This indicates that the appearance and development of tinnitus is a dynamic process involving aberrant local neural activity and abnormal connectivity in multifunctional brain networks.
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http://dx.doi.org/10.1002/hbm.25238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7776005PMC
February 2021

Phosphatase and Tensin Homolog (PTEN) of Japanese Flounder-Its Regulation by miRNA and Role in Autophagy, Apoptosis and Pathogen Infection.

Int J Mol Sci 2020 Oct 19;21(20). Epub 2020 Oct 19.

CAS Key Laboratory of Experimental Marine Biology, CAS Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.

MicroRNAs (miRNAs) are small non-coding RNAs with important roles in diverse biological processes including immunity. Japanese flounder () is an aquaculture fish species susceptible to the infection of bacterial and viral pathogens including . In a previous study, pol-miR-novel_547, a novel miRNA of flounder with unknown function, was found to be induced by . In the present study, we investigated the regulation and function of pol-miR-novel_547 and its target gene. We found that pol-miR-novel_547 was regulated differently by and the viral pathogen megalocytivirus, and pol-miR-novel_547 repressed the expression of PTEN (phosphatase and tensin homolog) of flounder (PoPTEN). PoPTEN is ubiquitously expressed in multiple tissues of flounder and responded to bacterial and viral infections. Interference with PoPTEN expression in flounder cells directly or via pol-miR-novel_547 promoted invasion. Consistently, in vivo knockdown of PoPTEN enhanced dissemination in flounder tissues, whereas in vivo overexpression of PoPTEN attenuated dissemination but facilitated megalocytivirus replication. Further in vitro and in vivo studies showed that PoPTEN affected autophagy activation via the AKT/mTOR pathway and also modulated the process of apoptosis. Together these results reveal for the first time a critical role of fish PTEN and its regulatory miRNA in pathogen infection, autophagy, and apoptosis.
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http://dx.doi.org/10.3390/ijms21207725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589652PMC
October 2020

Molecular epidemiological and hematological profile of thalassemia in the Dongguan Region of Guangdong Province, Southern China.

J Clin Lab Anal 2021 Feb 28;35(2):e23596. Epub 2020 Sep 28.

Department of Medical and Molecular Genetics, Dongguan Institute of Pediatrics, Dongguan, China.

Background: Thalassemia is a common inherited hematological disease in tropical and subtropical regions. This study aimed to investigate the mutation spectrum of thalassemia in the Dongguan region of southern China and comprehensively analyze hematologic features of thalassemia carriers with various types of globin mutations.

Methods: A hematological screening including hematological indices such as mean corpuscular volume (MCV), mean corpuscular hemoglobin content (MCH), and mean corpuscular hemoglobin concentration (MCHC) was conducted in 19 442 people from Dongguan region, Guangdong province of China. Then, 4891 suspected thalassemia carriers were further investigated by genetic analysis of combined NGS and gap-PCR.

Results: Totally, 2319 (11.9%) cases were diagnosed as carriers of thalassemia, of which 1483 cases (7.6%) were α-thalassemia, 741 cases (3.8%) were β-thalassemia, and 95 cases (0.5%) were co-inheritance of α- and β-thalassemia. In α-thalassemia carriers, the phenotypic severity increases with the number of nonfunctional α-globin genes. The patients with - /α α genotype have less severe clinical phenotypes than those with other Hb H diseases. As for β-thalassemia, the MCV and MCH in both β and β carriers are markedly reduced.

Conclusions: This is the first comprehensive molecular epidemiological survey and hematological profiling of thalassemia in Dongguan area. This study will be benefit for genetic counseling in the clinic and may help pediatricians to make a correct diagnosis of different types of thalassemia.
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http://dx.doi.org/10.1002/jcla.23596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891532PMC
February 2021

Impact of COVID-19 on Peripheral Arterial Disease Treatment.

Ann Vasc Surg 2020 08 2;67:6-7. Epub 2020 Jun 2.

Department of Vascular Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China. Electronic address:

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http://dx.doi.org/10.1016/j.avsg.2020.05.045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265821PMC
August 2020

A molecular-beacon-based asymmetric PCR assay for detecting polymorphisms related to folate metabolism.

J Clin Lab Anal 2020 Aug 15;34(8):e23337. Epub 2020 May 15.

Department of Medical and Molecular Genetics, Dongguan Institute of Pediatrics, Dongguan, China.

Background: Polymorphisms (rs1801133 or C677T; rs1801131 or A1298C) of the MTHFR gene and rs1801394 (A66G) of the MTRR gene are important genetic determinants of folate metabolism. A convenient, sensitive, and reliable method is required to detect polymorphisms for the precise supplementation of folate.

Methods: A rapid detection method based on molecular beacon probes that can detect rs1801133, rs1801131, and rs1801394 simultaneously was developed in this study. Specific primers and probes were designed, and the amplification system and conditions were optimized. We applied our method to a group of 500 unrelated women of gestational age in the Dongguan region of Guangdong Province in China. The clinical performance of this assay was evaluated by testing 94 samples in comparison with Sanger sequencing.

Results: The molecular-beacon-based PCR assay we established is extremely sensitive, with a detection limit of 2 ng/μL of genomic DNA, and validated by direct sequencing in a blind study with 100% concordance.

Conclusion: The results demonstrate that our molecular-beacon-based asymmetric PCR assay is an easy, reliable, high-yield, and cost-effective method for the simultaneous detection of three polymorphisms related to folate metabolism. It could help evaluate the risk of perinatal-neonatal neural tube malformation, pregnancy hypertension, and other diseases and guide the individualized supplementation of folic acid. Data on the spectrum of mutations in the Dongguan District in this study are beneficial for guiding the supplementation of folic acid.
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http://dx.doi.org/10.1002/jcla.23337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439423PMC
August 2020

Strategies and recommendations for the safe implementation of vascular surgery during the pandemic period of novel coronavirus pneumonia.

J Vasc Surg 2020 07 22;72(1):377-378. Epub 2020 Apr 22.

Department of Vascular Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

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http://dx.doi.org/10.1016/j.jvs.2020.04.466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175885PMC
July 2020

Reaction pathways and kinetics study on a syngas combustion system: CO + HO in an HO environment.

Phys Chem Chem Phys 2020 Mar;22(10):5797-5806

Key Laboratory of Advanced Technologies of Materials, Ministry of Education, and Institute of Material Dynamics, Southwest Jiaotong University, Chengdu, Sichuan 610031, P. R. China. and The Peac Institute of Multiscale Sciences, Chengdu, Sichuan 610031, P. R. China.

The reaction between CO and HO2 plays a significant role in syngas combustion. In this work, the catalytic effect of single-molecule water on this reaction is theoretically investigated at the CCSD(T)/aug-cc-pV(D,T,Q)Z and CCSD(T)-F12a/jun-cc-pVTZ levels in combination with the M062X/aug-cc-pVTZ level. Firstly, the potential energy surface (PES) of CO + HO2 (water-free) is revisited. The major products CO2 + OH are formed via a cis- or a trans-transition state (TS) channel and the formation of HCO + O2 is minor. In the presence of water, the title reaction has three different pre-reactive complexes (i.e., RC2: COHO2 + H2O, RC3: COH2O + HO2, and RC4: HO2H2O + CO), depending on the initial hydrogen bond formation. Compared to the water-free process, the reaction barriers of the water-assisted process are reduced considerably, due to more stable cyclic TSs and complexes. The rate constants for the bimolecular reaction pathways CO + HO2, RC2, RC3, and RC4 are further calculated using conventional transition state theory (TST) with Eckart asymmetric tunneling correction. For reaction CO + HO2, our calculations are in good agreement with the literature. In addition, the effective rate constants for the water-assisted process decrease by 1-2 orders of magnitude compared to the water-free one at a temperature below 600 K. In particular, the effective rate constants for the water-assisted and water-free processes are 1.55 × 10-28 and 3.86 × 10-26 cm3 molecule-1 s-1 at 300 K, respectively. This implies that the contribution of a single molecule water-assisted process is small and cannot accelerate the title reaction.
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http://dx.doi.org/10.1039/c9cp06642jDOI Listing
March 2020

IL‑37 expression is decreased in patients with hyperhomocysteinemia and protects cells from inflammatory injury by homocysteine.

Mol Med Rep 2020 Jan 6;21(1):371-378. Epub 2019 Nov 6.

Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.

As a novel anti‑inflammatory cytokine of the interleukin (IL)‑1 family, IL‑37 protects the human body from diseases characterized by excessive inflammation. The pathologic process of hyperhomocysteinemia (hHcy) is accompanied by persistent inflammation. However, little is known regarding the role of IL‑37 in hHcy. In the present study, the levels of cytokines including IL‑37, IL‑1β, IL‑6 and tumor necrosis factor‑α in the supernatant were detected by ELISA. mRNA and protein expression were detected by Reverse transcription‑quantitative PCR and western blotting, respectively. LDH level was determined by ELISA and the cell viability was detected through CCK‑8 kit. In the present study, mean serum IL‑37 levels of patients with hHcy were 32.3% lower than those of controls (P<0.01). In peripheral blood mononuclear cells (PBMCs) from patients with hHcy, mean IL‑37 mRNA expression was 73.5% lower (P<0.01) and IL‑37 protein expression was 77.7% lower compared with that of healthy controls (P<0.01). Furthermore, the results demonstrated that exogenous homocysteine (Hcy) stimulation markedly downregulated the mRNA and protein expression levels of IL‑37 in PBMCs in vitro. In 293T cells, overexpression of IL‑37 restored the cell viability impaired by Hcy, and reduced the release of lactate dehydrogenase and the proinflammatory cytokines IL‑1β, IL‑6 and tumor necrosis factor‑α. In conclusion, IL‑37 was downregulated by Hcy in vivo and in vitro, and IL‑37 exhibited a protective role against cell injury induced by Hcy.
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http://dx.doi.org/10.3892/mmr.2019.10804DOI Listing
January 2020

SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in .

Front Plant Sci 2019 30;10:1367. Epub 2019 Oct 30.

Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, China.

is one of the most widely used traditional Chinese medicinal plants because of its excellent performance in treating heart diseases. Tanshinones and phenolic acids are two important classes of effective metabolites, and their biosynthesis has attracted widespread interest. Here, we functionally characterized SmGRAS1 and SmGRAS2, two GRAS family transcription factors from . were highly expressed in the root periderm, where tanshinones mainly accumulated in . Overexpression of upregulated tanshinones accumulation and downregulated GA, phenolic acids contents, and root biomass. However, antisense expression of reduced the tanshinones accumulation and increased the GA, phenolic acids contents, and root biomass. The expression patterns of biosynthesis genes were consistent with the changes in compounds accumulation. GA treatment increased tanshinones, phenolic acids, and GA contents in the overexpression lines, and restored the root growth inhibited by overexpressing . Subsequently, yeast one-hybrid, dual-luciferase, and electrophoretic mobility shift assays (EMSA) showed SmGRAS1 promoted tanshinones biosynthesis by directly binding to the GARE motif in the promoter and activating its expression. Yeast two-hybrid assays showed SmGRAS1 interacted physically with SmGRAS2. Taken together, the results revealed that SmGRAS1/2 acted as repressors in root growth and phenolic acids biosynthesis but as positive regulators in tanshinones biosynthesis. Overall, our findings revealed the potential value of SmGRAS1/2 in genetically engineering changes in secondary metabolism.
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http://dx.doi.org/10.3389/fpls.2019.01367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831727PMC
October 2019

Inhibition of Brain Area and Functional Connectivity in Idiopathic Sudden Sensorineural Hearing Loss With Tinnitus, Based on Resting-State EEG.

Front Neurosci 2019 14;13:851. Epub 2019 Aug 14.

Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

This study aimed to identify the mechanism behind idiopathic sudden sensorineural hearing loss (ISSNHL) in patients with tinnitus by investigating aberrant activity in areas of the brain and functional connectivity. High-density electroencephalography (EEG) was used to investigate central nervous changes in 25 ISSNHL subjects and 27 healthy controls. ISSNHL subjects had significantly reduced activity in the left frontal lobe at the alpha 2 frequency band compared with controls. Linear lagged connectivity and lagged coherence analysis showed significantly reduced functional connectivity between the temporal gyrus and supramarginal gyrus at the gamma 2 frequency band in the ISSNHL group. Additionally, a significantly reduced functional connectivity was found between the central cingulate gyrus and frontal lobe under lagged phase synchronization analysis. These results strongly indicate inhibition of brain area activity and change in functional connectivity in ISSNHL with tinnitus patients.
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http://dx.doi.org/10.3389/fnins.2019.00851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702325PMC
August 2019

Physiological, transcriptional, and metabolic alterations in spaceflight-subjected Senna obtusifolia.

Plant Physiol Biochem 2019 Jun 9;139:33-43. Epub 2019 Mar 9.

Tianjin Tasly Modern TCM Resources Co. Ltd, Tianjin, 300400, China.

Senna obtusifolia is a widely used medicinal herb in Asian countries. To select elite cultivars, S. obtusifolia seeds were carried by "ShenZhou Ⅷ" recoverable satellite to space. Three spaceflight-subjected lines (SP-lines), namely QC10, QC29, QC46, and their ground control line (GC-line) were cultivated on the ground. Previous studies demonstrated that biological traits and secondary metabolites are different between SP-lines and GC-line. Here, we combined physiological, transcriptional, and metabolic studies to compare the differences between SP-lines and GC-line. The results showed that activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and monodehydroascorbate reductase (MDHAR) were dramatically increased in SP-lines as compared to that of GC-line. Transcript levels of SOD, POD, CAT, APX, and MDHAR were significantly up-regulated in SP-lines. Malondialdehyde (MDA) and hydrogen peroxide (HO) contents decreased in SP-lines. Seed yields of QC29 and QC46 were considerably higher than that of GC-line. Besides, QC29 had significantly higher aurantio-obtusin content. Pearson correlation coefficient analysis revealed positive relationships between POD and aurantio-obtusin, as well as APX and aurantio-obtusin. In conclusion, SP-lines have higher antioxidant gene expression level and antioxidant enzyme activity as compared to that of GC-line. With higher seed yield and aurantio-obtusin content, QC29 can be used to breed elite S. obtusifolia cultivars. This study provides a new insight in SP-lines and paves the way to breed elite S. obtusifolia cultivars in the future.
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http://dx.doi.org/10.1016/j.plaphy.2019.03.009DOI Listing
June 2019

The drug use to treat community-acquired pneumonia in children: A cross-sectional study in China.

Medicine (Baltimore) 2018 Nov;97(46):e13224

Department of Pharmacy.

To evaluate the rationality of drug use to treat community-acquired pneumonia (CAP) in children of a Chinese hospital using a set of developed indicators.We performed a retrospective cross-sectional study in West China Second University Hospital. Hospitalized children (0-18 years old) diagnosed with CAP from October 2015 to January 2016 were included. A set of developed indicators for assessing rational drug use (RDU) to treat CAP in children were used to evaluate the rationality of drug use. The data of the indicators were compared with the recommendations of the available guidelines, analyzing the situation of drug use in the children diagnosed with CAP.Eight hundred ninety-four children were included, 99.4% of them received antibiotics and 87.4% received more than 1 antibiotic. Antibiotics were administered intravenously in 880 (99.0%) children. About 20 (2.2%) children received antiviral agents and 19 (2.1%) children received antiviral drugs combined with antibiotics. About 208 (23.3%) children received traditional Chinese medicines and the injection of traditional Chinese medicines was given in 20 (2.2%) children.This study illustrated that drug use was partly not consistent with the recommendations of current guidelines, especially antibiotics. The drug use of CAP in children needs to pay more attention to.
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http://dx.doi.org/10.1097/MD.0000000000013224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257659PMC
November 2018

The ethylene response factor SmERF6 co-regulates the transcription of SmCPS1 and SmKSL1 and is involved in tanshinone biosynthesis in Salvia miltiorrhiza hairy roots.

Planta 2018 Jul 27;248(1):243-255. Epub 2018 Apr 27.

College of Life Science, Northwest A&F University, Yangling, 712100, China.

Main Conclusion: The SmERF6, which recognizes the GCC-box of SmCPS1 and SmKSL1 promoter in nucleus, regulates the tanshinone biosynthesis in Salvia miltiorrhiza hairy roots. Tanshinone, an important medicinal ingredient in Salvia miltiorrhiza, is best known for its use in medicine. However, the transcription factor regulation of tanshinone biosynthesis is unclear. Here, we isolated and identified a transcription factor in the ERF family of S. miltiorrhiza, SmERF6, which was screened from an S. miltiorrhiza cDNA library by the promoters of two key tanshinone synthesis genes (SmKSL1 and SmCPS1); this factor regulated tanshinone biosynthesis. The gene was highly expressed in the root and responded to ethylene treatment. SmERF6 modulated tanshinone biosynthesis by directly binding to an ethylene-responsive element (GCC-box) of the SmKSL1 and SmCPS1 promoters and activating their transcription. Overexpression of SmERF6 in the hairy roots increased their tanshinone accumulation, and SmERF6 silencing by RNAi led to a lower tanshinone content. Furthermore, tanshinone accumulation maintained homeostasis with the total phenolic acid and flavonoid contents in S. miltiorrhiza. These findings elucidated how SmERF6 directly co-regulates the transcription of SmCPS1 and SmKSL1 and modulates tanshinone synthesis to accelerate the metabolic flux of tanshinone accumulation in S. miltiorrhiza.
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http://dx.doi.org/10.1007/s00425-018-2884-zDOI Listing
July 2018

Is a patient-specific drill template via a cortical bone trajectory safe in cervical anterior transpedicular insertion?

J Orthop Surg Res 2018 Apr 18;13(1):91. Epub 2018 Apr 18.

Department of Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, School of Basic Medical Sciences, Southern Medical University, 1063 Shatai Nan Road, Baiyun District, Guangzhou, Guangdong, China.

Background: This study aimed to develop patient-specific drill templates by computer numerical control or three-dimensional printing via two cortical bone trajectories (CBTs) and to evaluate their efficacies and accuracies in cervical anterior transpedicular insertion.

Methods: Preoperative CT images of 20 cadaveric cervical vertebrae (C3-C7) were obtained. After image processing, patient-specific drill templates were randomly assigned to be constructed via two CBTs (CBT0 and CBT0.7) and manufactured by two methods (computer numerical control and three-dimensional printing). Guided by patient-specific drill templates, 3.5-mm-diameter screws were inserted into the pedicles. Postoperative CT scans were performed to evaluate the screw deviation in the entry point and midpoint of the pedicle. The screw positions were also graded.

Results: Computer numerical control patient-specific drill templates had a significantly shorter manufacturing time compared to three-dimensional-printed patient-specific drill templates (p < 0.01). Absolute deviations at the entry point and midpoint of the pedicle had no significant differences on the transverse and sagittal planes (p > 0.05). There were no significant differences in screw positions (p = 0.3). However, three screw positions were in grade 3 in CBT0, while the others were in grade 1.

Conclusions: CBT0.7 appears to be a safe and feasible trajectory for cervical anterior transpedicular insertion. Bio-safe computer numerical control patient-specific drill templates can facilitate cervical anterior transpedicular insertion with good feasibility and accuracy.
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http://dx.doi.org/10.1186/s13018-018-0810-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907449PMC
April 2018

Corticosteroids for the prevention of bronchopulmonary dysplasia in preterm infants: a network meta-analysis.

Arch Dis Child Fetal Neonatal Ed 2018 Nov 23;103(6):F506-F511. Epub 2018 Feb 23.

Department of Pharmacy, West China Second University Hospital, Sichuan University, Chengdu, China.

Objective: To determine the comparative efficacy and safety of corticosteroids in the prevention of bronchopulmonary dysplasia (BPD) in preterm infants.

Study Design: We systematically searched PubMed, EMBASE and the Cochrane Library. Two reviewers independently selected randomised controlled trials (RCTs) of postnatal corticosteroids in preterm infants. A Bayesian network meta-analysis and subgroup analyses were performed.

Results: We included 47 RCTs with 6747 participants. The use of dexamethasone at either high dose or low dose decreased the risk of BPD (OR 0.29, 95% credible interval (CrI) 0.14 to 0.52; OR 0.58, 95% CrI 0.39 to 0.76, respectively). High-dose dexamethasone was more effective than hydrocortisone, beclomethasone and low-dose dexamethasone. Early and long-term dexamethasone at either high dose or low dose decreased the risk of BPD (OR 0.11, 95% CrI 0.02 to 0.4; OR 0.37, 95% CrI 0.16 to 0.67, respectively). There were no statistically significant differences in the risk of cerebral palsy (CP) between different corticosteroids. However, high-dose and long-term dexamethasone ranked lower than placebo and other regimens in terms of CP. Subgroup analyses indicated budesonide was associated with a decreased risk of BPD in extremely preterm and extremely low birthweight infants (OR 0.60, 95% CrI 0.36 to 0.93).

Conclusions: Dexamethasone can reduce the risk of BPD in preterm infants. Of the different dexamethasone regimens, aggressive initiation seems beneficial, while a combination of high-dose and long-term use should be avoided because of the possible adverse neurodevelopmental outcome. Dexamethasone and inhaled corticosteroids need to be further evaluated in large-scale RCTs with long-term follow-ups.
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http://dx.doi.org/10.1136/archdischild-2017-313759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278655PMC
November 2018

Development of indicators for assessing rational drug use to treat community-acquired pneumonia in children in hospitals and clinics: A modified Delphi study.

Medicine (Baltimore) 2017 Dec;96(51):e9308

Department of Pharmacy Evidence-Based Pharmacy Center, West China Second University Hospital, Sichuan University Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education (Sichuan University) West China School of Pharmacy, Sichuan University Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, China.

Community-acquired pneumonia (CAP) is a common infectious disease in children. Rational drug use (RDU) is an important approach to reducing the disease burden and mortality rate of CAP in children. There are no monitoring indicators for assessing RDU in children. This study aimed to develop a set of indicators to assess RDU to treat CAP in children in hospitals and clinics using a modified Delphi method.Initial indicators were generated based on a systematic review of guidelines and studies investigating CAP in children. A 3-round modified Delphi process in the form of an email survey combined with round-table discussion was then carried out, and an analytic hierarchy process (AHP) was applied to determine the weight of each indicator.A total of 24 and 8 experts were invited to participate in the email survey and round-table discussion, respectively. A consensus was reached after 3 rounds of the Delphi survey. Three first-rank indicators and 23 second-rank indicators were developed, and each indicator was weighted. The first-rank indicators comprised drug choice (45.5%), drug usage and dosage (36.4%), and the duration of drug therapy (18.2%); the second-rank indicators were indicators related to antibiotics (63.6%), antiviral agents (18.2%), traditional Chinese medicines (4.5%), and adjuvant drugs (13.6%). The weight value of drug selection was the highest, followed by the values of drug usage and dosage and the duration of drug therapy.The developed indicator set constitutes the first set intended to assess RDU to treat CAP in children in hospitals (including community hospitals) and clinics. The indicators were based on drug selection, drug usage and dosage and duration of drug therapy, which are associated with most therapeutic drugs for CAP in children. Monitoring these indicators will guide people towards the promotion of RDU in the absence of drug monitoring indicators for CAP. Furthermore, the indicator set constitutes a methodological reference for the development of other indicator sets.
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http://dx.doi.org/10.1097/MD.0000000000009308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5758202PMC
December 2017
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