Publications by authors named "Wenlu Li"

97 Publications

Neural mechanism of noise affecting face recognition.

Neuroscience 2021 Jun 17. Epub 2021 Jun 17.

Brainnetome Center, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; National Laboratory of Pattern Recognition, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; School of Artificial Intelligence, University of Chinese Academy of Sciences, Beijing 100049, China; Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China; Key Laboratory for Neuro Information of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China; Queensland Brain Institute, University of Queensland 4072, Brisbane, Australia. Electronic address:

Face recognition is one of the most important cognitive functions for humans in social activities. The ability will be negatively affected when the face images deteriorate. However, the neural process of extracting facial information under challenging conditions is still poorly understood. Therefore, it is necessary to further understand the neurophysiological relevance of this effect. We examined patients with multiple subdural electrodes (ECoG) monitored for clinical purposes. During the experimental task, the patients were presented with face and house images with different noise levels and were asked to recognize the faces. We found a striking increase in high gamma band power (HGP; 60-160 Hz) when face images were shown. We localized the face-specific electrodes to the fusiform gyrus (FG) and surrounding cortices. For each subject, the behavioral performance and magnitudes of the HGP for the face-specific sites significantly both fit a sigmoid function and showed similar changes. Additionally, the curve profile of the average HGP magnitude across the face-specific sites was almost equal to the average behavior curve; the former could precisely track the behavioral performance. In general, these results suggest that the HGP in the FG is closely related to the performance of face image recognition.
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http://dx.doi.org/10.1016/j.neuroscience.2021.06.017DOI Listing
June 2021

Soybean residue based biochar prepared by ball milling assisted alkali activation to activate peroxydisulfate for the degradation of tetracycline.

J Colloid Interface Sci 2021 Oct 19;599:631-641. Epub 2021 Apr 19.

College of Environmental Science and Engineering, Hunan University, Lushan South Road, Yuelu District, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Lushan South Road, Yuelu District, Changsha 410082, PR China. Electronic address:

The advanced oxidation process (AOPs) has caused great concern in recent years. Among them, biochar has been widely studied as a catalyst for advanced oxidation process because of its low price and low environmental risk. In this study, a novel ball milling assisted KOH activation biochar (MKBC) was prepared and applied in peroxydisulfate (PDS) activation to degrade tetracycline hydrochloride (TC-H). In comparison with the oxidation (3.48%) by PDS alone and adsorption (36.19%) by MKBC alone, the removal rate of TC-H was increased to 84.15% in the MKBC/PDS system, indicating that MKBC can successfully activate PDS. Besides, the catalytic activity of the MKBC to activate PDS for the degradation of TC-H is 58.33% higher than that of pristine biochar (PBC). In addition, MKBC has outstanding stability that after three repeated experiments, the removal rate of TC-H by the MKBC/PDS system still remains 77.35%. Meanwhile, the mechanism was investigated that the singlet oxygen (O) seized the principal position in the degradation of TC-H in the PDS/MKBC system. This study explored a novel, solvent-free and economic method to propose this extraordinary biochar, which provided a new strategy for the future research of biochar.
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http://dx.doi.org/10.1016/j.jcis.2021.04.074DOI Listing
October 2021

Circadian Biology and Stroke.

Stroke 2021 Jun 4;52(6):2180-2190. Epub 2021 May 4.

CIRCA consortium (E.H.L., G.W.A., M.D., G.D., E.E., R.F., D.W.H., Y-G.H., X.J., E.B.K., S.L., W.L., D.S.L., I.L., E.T.M., M.A.M., M.N., D.R., S.S., J.L.S., F.A.J.L.S., M.S., S.T., F.Z., A.M.B.), Massachusetts General Hospital, Harvard Medical School, Boston.

Circadian biology modulates almost all aspects of mammalian physiology, disease, and response to therapies. Emerging data suggest that circadian biology may significantly affect the mechanisms of susceptibility, injury, recovery, and the response to therapy in stroke. In this review/perspective, we survey the accumulating literature and attempt to connect molecular, cellular, and physiological pathways in circadian biology to clinical consequences in stroke. Accounting for the complex and multifactorial effects of circadian rhythm may improve translational opportunities for stroke diagnostics and therapeutics.
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http://dx.doi.org/10.1161/STROKEAHA.120.031742DOI Listing
June 2021

Cloning and Characterization of , a Member of the Stress-Associated Protein Family in Common Wheat.

Front Plant Sci 2021 22;12:609351. Epub 2021 Mar 22.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Stress association proteins (SAPs) are A20/AN1 zinc-finger domain proteins, which play important roles in plant adaptation to abiotic stress and plant development. The functions of SAPs in some plants were reported, but little is known about it in wheat ( L.). In this study, we characterized a novel 2AN1-type stress association protein gene , which was mapped to chromosome 5A in wheat. Subcellular localization indicated that TaSAP7-A was distributed in the nucleus and cytoplasm. Unlike previously known A20/AN1-type SAP genes, was negatively regulated to abiotic stress tolerance. Overexpressing lines were hypersensitive to ABA, osmotic and salt stress at germination stage and post-germination stage. Overexpression of plants accelerated the detached leaves' chlorophyll degradation. Association analysis of haplotypes and agronomic traits showed that was significantly associated with higher chlorophyll content at jointing stage and grain-filling stage. These results jointly revealed that is related to the chlorophyll content in the leaves of and wheat. Both and experiments demonstrated that TaSAP7-A interacted with TaS10B, which was the component of regulatory subunit in 26S proteasome. In general, was a regulator of chlorophyll content, and favorable haplotypes should be helpful for improving plant chlorophyll content and grain yield of wheat.
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http://dx.doi.org/10.3389/fpls.2021.609351DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020846PMC
March 2021

Unique MIL-53(Fe)/PDI Supermolecule Composites: Z-Scheme Heterojunction and Covalent Bonds for Uprating Photocatalytic Performance.

ACS Appl Mater Interfaces 2021 Apr 1;13(14):16364-16373. Epub 2021 Apr 1.

College of Environmental Science and Engineering, Hunan University, Lushan South Road, Yuelu District, Changsha 410082, People's Republic of China.

It is important to find an effective way to enhance the photocatalytic efficiency of metal-organic frameworks. In this work, an organic supermolecule perylene diimide (PDI) semiconductor with a carboxyl terminal was added into the synthesis process of MIL-53(Fe) crystals. The PDI/MIL-53(Fe) (PM) composite photocatalyst was first obtained. The TC-H photodegradation rate of the most efficient 5PM was nearly 94.08% within 30 min, whose apparent reaction rate constant () is 4 times that of PDI and 33 times that of MIL-53(Fe), respectively. By investigation and characterization, it has been found that PDI nanofibers were dispersed and fixed in MIL-53(Fe) and bonded to each other by covalent bonds. The radical trap experiments and electron spin resonance analysis illustrated that hydroxyl radical (·OH), superoxide radical (·O), and photogenerated holes (h) were active species. Combined with the band structure of PDI and MIL-53(Fe), it is proposed that the PM photocatalyst was a Z-scheme heterojunction mechanism. Therefore, PM photocatalysts showed excellent charge separation and transfer ability. The performance improvement of 5PM is due to enhanced visible light absorption, efficient charge separation, and excellent redox potential. Five cyclic photocatalytic tests and experiments further demonstrate that the 5PM photocatalyst has a promising future for pollutant removal.
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http://dx.doi.org/10.1021/acsami.1c01308DOI Listing
April 2021

Driving style recognition and comparisons among driving tasks based on driver behavior in the online car-hailing industry.

Accid Anal Prev 2021 May 23;154:106096. Epub 2021 Mar 23.

Jiangsu Key Laboratory of Urban ITS, School of Transportation, Southeast University, No. 2, Southeast University Road, Jiangning District, Nanjing, 211189, China. Electronic address:

As a product of the shared economy, online car-hailing platforms can be used effectively to help maximize resources and alleviate traffic congestion. The driver's behavior is characterized by his or her driving style and plays an important role in traffic safety. This paper proposes a novel framework to classify driving styles (defined as aggressive, normal, and cautious) based on online car-hailing data to investigate the distinct characteristics of drivers when performing various driving tasks (defined as cruising, ride requests, and drop-off) and undergoing certain maneuvers (defined as turning, acceleration, and deceleration). The proposed model is constructed based on the detection and classification of driving maneuvers using a threshold-based endpoint detection approach, principal component analysis, and k-means clustering. The driving styles that the driver exhibits for the different driving tasks are compared and analyzed based on the classified maneuvers. The empirical results for Nanjing, China demonstrate that the proposed framework can detect driving maneuvers and classify driving styles accurately. Moreover, according to this framework, driving tasks lead to variations in driving style, and the variations in driving style during the different driving tasks differ significantly for turning, acceleration, and deceleration maneuvers.
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http://dx.doi.org/10.1016/j.aap.2021.106096DOI Listing
May 2021

ErbB3 is a critical regulator of cytoskeletal dynamics in brain microvascular endothelial cells: Implications for vascular remodeling and blood-brain-barrier modulation.

J Cereb Blood Flow Metab 2021 Feb 14:271678X20984976. Epub 2021 Feb 14.

Neuroprotection Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, USA.

Neuregulin (NRG)1 - ErbB receptor signaling has been shown to play an important role in the biological function of peripheral microvascular endothelial cells. However, little is known about how NRG1/ErbB signaling impacts brain endothelial function and blood-brain barrier (BBB) properties. NRG1/ErbB pathways are affected by brain injury; when brain trauma was induced in mice in a controlled cortical impact model, endothelial ErbB3 gene expression was reduced to a greater extent than that of other NRG1 receptors. This finding suggests that ErbB3-mediated processes may be significantly compromised after injury, and that an understanding of ErbB3 function would be important in the of study of endothelial biology in the healthy and injured brain. Towards this goal, cultured brain microvascular endothelial cells were transfected with siRNA to ErbB3, resulting in alterations in F-actin organization and microtubule assembly, cell morphology, migration and angiogenic processes. Importantly, a significant increase in barrier permeability was observed when ErbB3 was downregulated, suggesting ErbB3 involvement in BBB regulation. Overall, these results indicate that neuregulin-1/ErbB3 signaling is intricately connected with the cytoskeletal processes of the brain endothelium and contributes to morphological and angiogenic changes as well as to BBB integrity.
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http://dx.doi.org/10.1177/0271678X20984976DOI Listing
February 2021

Blood-Brain Barrier Mechanisms in Stroke and Trauma.

Handb Exp Pharmacol 2021 Feb 13. Epub 2021 Feb 13.

Neuroprotection Research Laboratories, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

The brain microenvironment is tightly regulated. The blood-brain barrier (BBB), which is composed of cerebral endothelial cells, astrocytes, and pericytes, plays an important role in maintaining the brain homeostasis by regulating the transport of both beneficial and detrimental substances between circulating blood and brain parenchyma. After brain injury and disease, BBB tightness becomes dysregulated, thus leading to inflammation and secondary brain damage. In this chapter, we overview the fundamental mechanisms of BBB damage and repair after stroke and traumatic brain injury (TBI). Understanding these mechanisms may lead to therapeutic opportunities for brain injury.
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http://dx.doi.org/10.1007/164_2020_426DOI Listing
February 2021

Steering Electron-Hole Migration Pathways Using Oxygen Vacancies in Tungsten Oxides to Enhance Their Photocatalytic Oxygen Evolution Performance.

Angew Chem Int Ed Engl 2021 Apr 4;60(15):8236-8242. Epub 2021 Mar 4.

Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China.

The overall water splitting efficiency is mainly restricted by the slow kinetics of oxygen evolution. Therefore, it is essential to develop active oxygen evolution catalysts. In this context, we designed and synthesized a tungsten oxide catalyst with oxygen vacancies for photocatalytic oxygen evolution, which exhibited a higher oxygen evolution rate of 683 μmol h  g than that of pure WO (159 μmol h  g ). Subsequent studies through transient absorption spectroscopy found that the oxygen vacancies can produce electron trapping states to inhibit the direct recombination of photogenerated carriers. Additionally, a Pt cocatalyst can promote electron trap states to participate in the reaction to improve the photocatalytic performance further. This work uses femtosecond transient absorption spectroscopy to explain the photocatalytic oxygen evolution mechanism of inorganic materials and provides new insights into the design of high-efficiency water-splitting catalysts.
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http://dx.doi.org/10.1002/anie.202016170DOI Listing
April 2021

MiR-770 promotes oral squamous cell carcinoma migration and invasion by regulating the Sirt7/Smad4 pathway.

IUBMB Life 2021 Jan 16;73(1):264-272. Epub 2020 Dec 16.

Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Oral squamous cell carcinoma (OSCC) is a common malignant cancer with unfavorable prognosis, and the epithelial-to-mesenchymal transition (EMT) is a critical contributor to OSCC metastasis. Recently, we have shown that sirtuin 7 (Sirt7) is associated with EMT and OSCC metastasis by acetylating small mother against decapentaplegic 4 (Smad4). Nonetheless, the mechanism of Sirt7 downregulation in OSCC cells remains unknown. This study analyzed the potential microRNAs that were predicted to regulate Sirt7 expression by online databases. We identified miR-770 as an upstream regulator of Sirt7 that targets its 3'-untranslated region. The expression of miR-770 was observed to be negatively correlated with the mRNA expression of Sirt7 in metastatic OSCC tumors, and higher miR-770 expression was correlated with poorer OSCC patient survival. Our in vitro data indicated that miR-770 promoted OSCC cell migration and invasion, and this process was dependent on Sirt7/Smad4 signaling. Furthermore, in vivo metastasis experiments indicated that miR-770 overexpression led to more prominent OSCC metastasis and downregulated Sirt7 expression. Collectively, our results revealed a new role of Sirt7 downregulation in metastatic OSCC and suggested that miR-770 is a potential target in counteracting OSCC metastasis.
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http://dx.doi.org/10.1002/iub.2426DOI Listing
January 2021

EphrinB2-EphB2 signaling for dendrite protection after neuronal ischemia and oxygen-glucose deprivation .

J Cereb Blood Flow Metab 2021 Jul 16;41(7):1744-1755. Epub 2020 Dec 16.

Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA.

In order to rescue neuronal function, neuroprotection should be required not only for the neuron soma but also the dendrites. Here, we propose the hypothesis that ephrin-B2-EphB2 signaling may be involved in dendritic degeneration after ischemic injury. A mouse model of focal cerebral ischemia with middle cerebral artery occlusion (MCAO) method was used for EphB2 signaling test in vivo. Primary cortical neuron culture and oxygen-glucose deprivation were used to assess EphB2 signaling in vitro. siRNA and soluble ephrin-B2 ectodomain were used to block ephrin-B2-Ephb2 signaling. In the mouse model of focal cerebral ischemia and in neurons subjected to oxygen-glucose deprivation, clustering of ephrin-B2 with its receptor EphB2 was detected. Phosphorylation of EphB2 suggested activation of this signaling pathway. RNA silencing of EphB2 prevented neuronal death and preserved dendritic length. To assess therapeutic potential, we compared the soluble EphB2 ectodomain with the NMDA antagonist MK801 in neurons after oxygen-glucose deprivation. Both agents equally reduced lactate dehydrogenase release as a general marker of neurotoxicity. However, only soluble EphB2 ectodomain protected the dendrites. These findings provide a proof of concept that ephrin-B2-EphB2 signaling may represent a novel therapeutic target to protect both the neuron soma as well as dendrites against ischemic injury.
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http://dx.doi.org/10.1177/0271678X20973119DOI Listing
July 2021

A graphene oxide Cookbook: Exploring chemical and colloidal properties as a function of synthesis parameters.

J Colloid Interface Sci 2021 Apr 30;588:725-736. Epub 2020 Nov 30.

Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, MO 63130, USA; Department of Chemical and Environmental Engineering, Yale University, CT 06520, USA. Electronic address:

Herein, we describe the synthesis of graphene oxide (GO) over a large range of conditions, exploring the effects of reaction temperature, reaction time, oxidant ratio, and sonication time on the chemical and colloidal properties of the product. As a function of reaction parameters, modified from Hummers' method, GO products were characterized and described via a suite of spectroscopic, structural, and morphological techniques, including TEM, UV-vis spectroscopy, XPS, Raman spectroscopy, FTIR, and DLS. Average carbon oxidation state and the yield (upon sonication) were chosen as the two criteria to evaluate synthesized GO materials. It was observed that as reaction temperature increased, GO oxidation state and yield of the sonication step both increased. Further, increasing reaction time and oxidant ratio not only increased the oxidation state, but also had a pronounced effect on the final yield. As synthesized, GO with higher degrees of oxidization exhibited higher negative ζ-potential, slightly smaller hydrodynamic diameter, and higher critical coagulation concentration(s). Data sets collectively demonstrate that carbon oxidation state, functional group ratios, and the aggregation kinetics of GO products can be readily controlled by varying processing time and conditions with expected changes in aqueous behavior(s), including stability/aggregation.
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http://dx.doi.org/10.1016/j.jcis.2020.11.102DOI Listing
April 2021

Rapid removal of organic pollutants by a novel persulfate/brochantite system: Mechanism and implication.

J Colloid Interface Sci 2021 Mar 8;585:400-407. Epub 2020 Dec 8.

College of Environmental Science and Engineering, Hunan University, Lushan South Road, Yuelu District, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Lushan South Road, Yuelu District, Changsha 410082, PR China.

Using natural minerals as persulfate activators can develop effective and economical in situ chemical oxidation technology for environmental remediation. Yet, few natural minerals can provide a high activation efficiency. Here, we demonstrate that brochantite (CuSO(OH)), a natural mineral, can be used as a persulfate activator for the rapid degradation of tetracycline hydrochloride (TC-H). Approximately 70% of TC-H was removed in CuSO(OH)/PDS within 5 min, which much higher than that of CuP (61.99%), CuO (29.75%), CNT (25.83%), FeO, (14.48%) and MnO (9.76%). Experiments and theoretical calculations suggested that surface copper acts as active sites induce the production of free radicals. The synergistic effect of Cu/S promotes the cycle between Cu/Cu. Sulfate radicals and hydroxyl radicals are the main reactive oxygen species that are responsible for the rapid removal of TC-H. The findings of this work show a novel persulfate/brochantite system and provide useful information for the environmental remediation.
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http://dx.doi.org/10.1016/j.jcis.2020.11.106DOI Listing
March 2021

Intrapore energy barriers govern ion transport and selectivity of desalination membranes.

Sci Adv 2020 Nov 25;6(48). Epub 2020 Nov 25.

Department of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA.

State-of-the-art desalination membranes exhibit high water-salt selectivity, but their ability to discriminate between ions is limited. Elucidating the fundamental mechanisms underlying ion transport and selectivity in subnanometer pores is therefore imperative for the development of ion-selective membranes. Here, we compare the overall energy barrier for salt transport and energy barriers for individual ion transport, showing that cations and anions traverse the membrane pore in an independent manner. Supported by density functional theory simulations, we demonstrate that electrostatic interactions between permeating counterion and fixed charges on the membrane substantially hinder intrapore diffusion. Furthermore, using quartz crystal microbalance, we break down the contributions of partitioning at the pore mouth and intrapore diffusion to the overall energy barrier for salt transport. Overall, our results indicate that intrapore diffusion governs salt transport through subnanometer pores due to ion-pore wall interactions, providing the scientific base for the design of membranes with high ion-ion selectivity.
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http://dx.doi.org/10.1126/sciadv.abd9045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688318PMC
November 2020

Transcriptomic characterization of microglia activation in a rat model of ischemic stroke.

J Cereb Blood Flow Metab 2020 12 17;40(1_suppl):S34-S48. Epub 2020 Jun 17.

Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA.

Microglia are key regulators of inflammatory response after stroke and brain injury. To better understand activation of microglia as well as their phenotypic diversity after ischemic stroke, we profiled the transcriptome of microglia after 75 min transient focal cerebral ischemia in 3-month- and 12-month-old male spontaneously hypertensive rats. Microglia were isolated from the brains by FACS sorting on days 3 and 14 after cerebral ischemia. GeneChip Rat 1.0ST microarray was used to profile the whole transcriptome of sorted microglia. We identified an evolving and complex pattern of activation from 3 to 14 days after stroke onset. M2-like patterns were extensively and persistently upregulated over time. M1-like patterns were only mildly upregulated, mostly at day 14. Younger 3-month-old brains showed a larger microglial response in both pro- and anti-inflammatory pathways, compared to older 12-month-old brains. Importantly, our data revealed that after stroke, most microglia are activated towards a wide spectrum of novel polarization states beyond the standard M1/M2 dichotomy, especially in pathways related to TLR2 and dietary fatty acid signaling. Finally, classes of transcription factors that might potentially regulate microglial activation were identified. These findings should provide a comprehensive database for dissecting microglial mechanisms and pursuing neuroinflammation targets for acute ischemic stroke.
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http://dx.doi.org/10.1177/0271678X20932870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687036PMC
December 2020

Effects of O-GlcNAcylation on functional mitochondrial transfer from astrocytes.

J Cereb Blood Flow Metab 2021 Jul 5;41(7):1523-1535. Epub 2020 Nov 5.

Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA.

Mitochondria may be transferred from cell to cell in the central nervous system and this process may help defend neurons against injury and disease. But how mitochondria maintain their functionality during the process of release into extracellular space remains unknown. Here, we report that mitochondrial protein O-GlcNAcylation is a critical process to support extracellular mitochondrial functionality. Activation of CD38-cADPR signaling in astrocytes robustly induced protein O-GlcNAcylation in mitochondria, while oxygen-glucose deprivation and reoxygenation showed transient and mild protein modification. Blocking the endoplasmic reticulum - Golgi trafficking with Brefeldin A or slc35B4 siRNA reduced O-GlcNAcylation, and resulted in the secretion of mitochondria with decreased membrane potential and mtDNA. Finally, loss-of-function studies verified that O-GlcNAc-modified mitochondria demonstrated higher levels of neuroprotection after astrocyte-to-neuron mitochondrial transfer. Collectively, these findings suggest that post-translational modification by O-GlcNAc may be required for supporting the functionality and neuroprotective properties of mitochondria released from astrocytes.
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http://dx.doi.org/10.1177/0271678X20969588DOI Listing
July 2021

Assessment of Quality of Life of Free Anterolateral Thigh Flap for Reconstruction of Tissue Defects of Total or Near-Total Glossectomy.

J Oncol 2020 10;2020:2920418. Epub 2020 Oct 10.

Department of Oral and Maxillofacial Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.

Background: The aim of this study was to evaluate quality of life of free anterolateral thigh flap (ALTFF) for reconstruction of tissue defects of total or near-total glossectomy.

Methods: Quality of life was assessed by means of the University of Washington Quality of Life (UW-QOL) and the 14-item Oral Health Impact Profile (OHIP-14), after 12 months postoperatively.

Results: 65 of 79 questionnaires were returned (82.27%). In the UW-QOL, the best-scoring domain was "shoulder," whereas the lowest scores were for "chewing" and "pain." In the OHIP-14, the lowest-scoring domain was "handicap," followed by "Social disability" and "Psychological disability."

Conclusion: Free anterolateral thigh perforator flaps for reconstruction of total or near-total glossectomy defects after cancer resection would have significantly influenced the patients' oral functions and quality of life.
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http://dx.doi.org/10.1155/2020/2920418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569442PMC
October 2020

HOXC13-AS accelerates cell proliferation and migration in oral squamous cell carcinoma via miR-378g/HOXC13 axis.

Oral Oncol 2020 12 4;111:104946. Epub 2020 Aug 4.

Department of Stomatology, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Road, Zhengzhou 450000, Henan, China.

Oral squamous cell carcinoma (OSCC) is an aggressive cancer type in head and neck. A number of long non-coding RNAs (lncRNAs) are discovered to serve regulatory roles in OSCC. HOXC13 antisense RNA (HOXC13-AS) has been proved to behave as a tumor-facilitator in nasopharyngeal carcinoma, but its regulatory role in OSCC has never been investigated. In this study, GEPIA indicated that HOXC13-AS and its neighbor gene HOXC13 were upregulated in HNSC samples, and we consistently unveiled their upregulation in OSCC tissues and cell lines. Silencing HOXC13-AS abrogated OSCC cell proliferation, migration, and epithelial-to-mesenchymal transition (EMT). Moreover, HOXC13 overexpression rescued the influences of HOXC13-AS silence on OSCC cellular processes and in vivo tumor growth. Mechanistically, HOXC13-AS upregulated HOXC13 expression in OSCC through sequestering miR-378g, which was proved to exert suppressive functions in the malignant behaviors of OSCC cells. Further, HOXC13 was revealed to be positively correlated with HOXC13-AS and negatively with miR-378g in expression in OSCC samples. In sum, our findings suggested that HOXC13-AS functioned as a ceRNA to accelerate the malignant behaviors of OSCC cells via miR-378g/HOXC13 axis, shedding a new light on the lncRNA-targeted treatment for OSCC.
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http://dx.doi.org/10.1016/j.oraloncology.2020.104946DOI Listing
December 2020

Interfacial internal electric field and oxygen vacancies synergistically enhance photocatalytic performance of bismuth oxychloride.

J Hazard Mater 2021 Jan 15;402:123470. Epub 2020 Jul 15.

Department of Chemistry, Tsinghua University, Beijing, 100084, PR China. Electronic address:

Solar-to-chemical energy conversion is valuable and sustainable strategy for energy and environmental crisis through photocatalysis. The amorphous SnO modified BiOCl (Sn-BiOCl) full-spectrum-responsive catalysts were designed and synthesized through solvothermal method. The introduced Sn regulates the growth of BiOCl to form ultrathin nanosheets with surface oxygen vacancies. And the surface modification of SnO induces interfacial internal electric field via charge redistribution on the interface of BiOCl and SnO to accelerate the photogenerated charge separation. The modification of SnO decreased work function of Sn-BiOCl and thus elevated its conduction band and valence band simultaneously, leading enhanced photocatalytic reducibility with the improved generation rate of ·O. The surface SnO and oxygen vacancies of Sn-BiOCl broadened light absorption range and enhanced photocatalytic performance synergistically, resulting in 14-fold increased photodegradation rate of phenol compared with pure BiOCl under full spectrum. This method is also able to expand to other metal ions (such as Fe, In and Sb). This work provides a valuable concept in structure regulating for enhanced photocatalytic performance in the removal of organic pollutants by interfacial internal electric field and surface oxygen vacancies.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123470DOI Listing
January 2021

Leaky memories: Impact of on blood-brain barrier and dementia.

Authors:
Wenlu Li Eng H Lo

J Cereb Blood Flow Metab 2020 09 29;40(9):1912-1914. Epub 2020 Jun 29.

Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

A new study suggests that the leading genetic risk factor for Alzheimer's disease, apolipoprotein E4 (), is linked to blood-brain barrier breakdown and subsequent cognitive decline. These findings broaden our understanding of how cerebrovascular mechanisms contribute to cognitive impairment and should stimulate new directions for pursuing therapeutic approaches for Alzheimer's disease and related dementias.
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http://dx.doi.org/10.1177/0271678X20938146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7446559PMC
September 2020

Author Correction: Potential circadian effects on translational failure for neuroprotection.

Nature 2020 Jul;583(7814):E14

Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41586-020-2427-1DOI Listing
July 2020

Potential circadian effects on translational failure for neuroprotection.

Nature 2020 06 3;582(7812):395-398. Epub 2020 Jun 3.

Neuroprotection Research Laboratories, Departments of Radiology and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Neuroprotectant strategies that have worked in rodent models of stroke have failed to provide protection in clinical trials. Here we show that the opposite circadian cycles in nocturnal rodents versus diurnal humans may contribute to this failure in translation. We tested three independent neuroprotective approaches-normobaric hyperoxia, the free radical scavenger α-phenyl-butyl-tert-nitrone (αPBN), and the N-methyl-D-aspartic acid (NMDA) antagonist MK801-in mouse and rat models of focal cerebral ischaemia. All three treatments reduced infarction in day-time (inactive phase) rodent models of stroke, but not in night-time (active phase) rodent models of stroke, which match the phase (active, day-time) during which most strokes occur in clinical trials. Laser-speckle imaging showed that the penumbra of cerebral ischaemia was narrower in the active-phase mouse model than in the inactive-phase model. The smaller penumbra was associated with a lower density of terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive dying cells and reduced infarct growth from 12 to 72 h. When we induced circadian-like cycles in primary mouse neurons, deprivation of oxygen and glucose triggered a smaller release of glutamate and reactive oxygen species, as well as lower activation of apoptotic and necroptotic mediators, in 'active-phase' than in 'inactive-phase' rodent neurons. αPBN and MK801 reduced neuronal death only in 'inactive-phase' neurons. These findings suggest that the influence of circadian rhythm on neuroprotection must be considered for translational studies in stroke and central nervous system diseases.
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http://dx.doi.org/10.1038/s41586-020-2348-zDOI Listing
June 2020

From in vitro to in vivo reprogramming for neural transdifferentiation: An approach for CNS tissue remodeling using stem cell technology.

J Cereb Blood Flow Metab 2020 09 19;40(9):1739-1751. Epub 2020 May 19.

iPSC-based Drug Discovery and Development Team, RIKEN BioResource Research Center (BRC), Kyoto, Japan.

Advances in stem cell technology have provided three approaches to address the demanding issue of the treatment of intractable neurological disease. One of the approaches is the screening of compounds attenuating pathological phenotypes in stem-cell based models. A second approach consists of exogenous-targeted cell supplementation to the lesion with stem cell-derived differentiated cells. A third approach involves in vivo direct programming to transdifferentiate endogenous somatic cells and to boost CNS tissue remodeling. In this review, we outline research advances in stem cell technology of direct reprogramming in vitro and in vivo and discuss the future challenge of tissue remodeling by neural transdifferentiation.
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http://dx.doi.org/10.1177/0271678X20910324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7446571PMC
September 2020

Formation and Transport of Cr(III)-NOM-Fe Colloids upon Reaction of Cr(VI) with NOM-Fe(II) Colloids at Anoxic-Oxic Interfaces.

Environ Sci Technol 2020 04 20;54(7):4256-4266. Epub 2020 Mar 20.

Department of Energy, Environmental, and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.

Natural organic matter-iron (NOM-Fe) colloids are ubiquitous at anoxic-oxic interfaces of subsurface environments. Fe(II) or NOM can chemically reduce Cr(VI) to Cr(III), and the formation of Cr(III)-NOM-Fe colloids can control the fate and transport of Cr. We explored the formation and transport of Cr(III)-humic acid (HA)-Fe colloids upon reaction of Cr(VI) with HA-Fe(II) colloids over a range of environmentally relevant conditions. Cr(VI) was completely reduced by HA-Fe(II) complexes under anoxic conditions, and the formation of Cr(III)-HA-Fe colloids depended on HA concentration (or molar C/Fe ratio) and redox conditions. No colloids formed at HA concentrations below 3.5 mg C/L (C/Fe ratio below 1.6), but Cr(III)-HA-Fe colloids formed at higher HA concentrations. In column experiments, Cr(III)-HA-Fe(III) colloids formed under oxic conditions were readily transported through sand-packed porous media. Colloidal stability measurements further suggest that Cr(III)-HA-Fe colloids are highly stable and persist for at least 20 days without substantial change in particle size. This stability is attributed to the enrichment of free HA adsorbed on the Cr(III)-HA-Fe colloid surfaces, intensifying the electrostatic and/or steric repulsion interactions between particles. The new insights provided here are important for evaluating the long-term fate and transport of Cr in organic-rich redox transition zones.
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http://dx.doi.org/10.1021/acs.est.9b07934DOI Listing
April 2020

Impact of the Body Mass Index on Hemorrhage After Surgery for Thyroid Cancer.

Cancer Manag Res 2020 23;12:557-565. Epub 2020 Jan 23.

Department of Head Neck and Thyroid Surgery, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China.

Objective: To investigate the effects of different values of the body mass index (BMI) on postoperative hemorrhage (PH) in thyroid cancer (TC) and its clinical management.

Methods: This retrospective cohort study selected 43 patients with hemorrhage after TC surgery in 7413 cases. Patients were divided based on the BMI (kg/m) into normal (24), overweight group (24 ≤BMl<28) and obese (≥28) groups. Clinical and pathologic data, bleeding cause, bleeding site, treatment and prognosis were assessed.

Results: BMI (=0.038) is an independent risk factor for PH of TC, related to hypertension (=0.004) and coronary heart disease (=0.001) in the three groups. Preoperative weight loss was not noted (=0.477). Hemorrhage in 60.47% of patients occurred between 4 h and 8 h after surgery. The higher the BMI, the longer was the operative time (≥1 h, 65.12%) (=0.017), which resulted in greater intraoperative blood loss (≥20 mL, 74.42%) (=0.025), postoperative hypoparathyroidism (=0.015) and the probability of injury to the recurrent laryngeal nerve (=0.026). The main causes of bleeding were incomplete vascular ligation (30.23%), severe postoperative cervical activity (16.28%) and long-term use of anticoagulant drugs (11.63%). Overall survival at 5 years in the obese group was poor (=0.015). Forty patients (93.02%) underwent surgical exploration and hemostasis and two patients (4.65%) underwent tracheotomy. All PH complications disappeared completely after active postoperative treatment, and all patients were discharged from hospital.

Conclusion: Obesity is closely associated with PH in TC patients. Therefore, in obese patients, active prevention preoperatively, complete hemostasis intraoperatively, early detection and timely treatment postoperatively are the key factors to reduce PH risk.
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http://dx.doi.org/10.2147/CMAR.S239264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986174PMC
January 2020

Characteristics and disinfection byproducts formation potential of dissolved organic matter released from fast-growing Eucalyptus urophylla leaves.

Chemosphere 2020 Jun 27;248:126017. Epub 2020 Jan 27.

School of Resources, Environment and Materials, Guangxi University, Nanning, 530004, China.

Every year, the harvesting of Eucalyptus generates a large amount of abandoned Eucalyptus leaves (ELs), which may release dissolved organic matter (DOM) when immersed in water. If these substances are carried by surface runoff directly to the source of drinking water, some components in the DOM tend to form disinfection byproducts (DBPs) within the water-supply system, posing risk to human health. In this study, the characteristics of DOM released from leaves of Eucalyptus urophylla were studied and the potential of DBPs formation of the EL-released DOM during the chlorination process was investigated. The results showed that the EL-released DOM was mainly composed of small molecules and hydrophobic substances. Of the total EL-released DOM, the proportion with molecular weight less than 10 kDa accounted for over 80% and the hydrophobic substances took up over 62%. The DOM showed strong absorbance at UV and the fluorescence response corresponding to humic acid-like (HA-like) fractions, soluble microbial byproduct-like, aromatic protein and fulvic acid-like (FA-like) material, which have been considered to be related to the potential precursors of chlorinated DBPs. Non-targeted screening demonstrated the presence of phenolics, carbohydrates, and amino acids. The analysis of products generated in chlorination process revealed the formation of trichloromethane (TCM) and the total organic halogen (TOX). The present study fully confirms that the DOM released from Eucalyptus urophylla leaves has great potential for the generation of chlorinated DBPs.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126017DOI Listing
June 2020

LncRNA LINC00662 promotes colon cancer tumor growth and metastasis by competitively binding with miR-340-5p to regulate CLDN8/IL22 co-expression and activating ERK signaling pathway.

J Exp Clin Cancer Res 2020 Jan 3;39(1). Epub 2020 Jan 3.

Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, 45000, Henan Province, China.

Background: LncRNA LINC00662 is closely related to the occurrence and development of cancer. This study aims to explore the effect of LINC00662 on colon cancer tumor growth and metastasis and its molecular mechanism.

Methods: CCK8, colony formation, transwell, scratch wound, TUNEL, flow cytometry, RT-PCR, western blotting and immunohistochemistry assays were used to detect the proliferation, apoptosis, invasion and migration of colon cancer cell and mRNA and protein expressions. Luciferase reporter and RNA pull down assays were used to detect the combination of LINC00662 and miR-340-5p or IL22 and the combination of miR-340-5p and CLDN8/IL22. Co-immunoprecipitation were used to detect the co-expression of CLDN8 and IL22 in colon cell lines. The targets of LINC00662 were predicated by Starbase v2.0. The target genes of miR-340-5p were predicated by miRDB and TargetScan. GO and KEGG enrichment analysis were performed by DAVID website.

Results: LINC00662 was up-regulation in colon cancer tissues and cell lines. Univariate Cox regression analysis showed that the LINC00662 expression level was related to the poor prognosis. LINC00662-WT and miR-340-5p mimics co-transfection depressed luciferase activity and IL22/CLDN8-WT and miR-340-5p inhibitors co-transfection memorably motivated luciferase activity. LINC00662 overexpression promoted cell proliferation, invasion and migration, and inhibited cell apoptosis in colon cancer. In vivo xenograft studies in nude mice manifested that LINC00662 overexpression prominently accelerate tumor growth. There was an opposite reaction in the biological functions of colon cells and tumor growth between LINC00662 overexpression and LINC00662 inhibition in vitro and in vivo. The functions of miR-340-5p mimics regulating the biological functions of colon cells and tumor growth were consistent with those of LINC00662 inhibition. CLDN8 and IL22, as target genes of miR-340-5p, reversed the functions of LINC00662 affecting the biological functions of colon cells and the protein levels of Bax, Bcl-2, XIAP, VEGF, MMP-2, E-cadherin and N-cadherin. Co-immunoprecipitation experiments indicated that CLDN8 directly interact with IL22 in colon cell lines. LINC00662 regulated CLDN8 and IL22 expressions and the activation of ERK signaling pathway via targeting miR-340-5p.

Conclusion: LINC00662 overexpression promoted the occurrence and development of colon cancer by competitively binding with miR-340-5p to regulate CLDN8/IL22 co-expression and activating ERK signaling pathway.
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http://dx.doi.org/10.1186/s13046-019-1510-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942292PMC
January 2020

Vascular Macrophages in Atherosclerosis.

J Immunol Res 2019 1;2019:4354786. Epub 2019 Dec 1.

Department of Surgical Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Atherosclerosis is the main pathological basis for the occurrence of most cardiovascular diseases, the leading global health threat, and a great burden for society. It has been well established that atherosclerosis is not only a metabolic disorder but also a chronic, sterile, and maladaptive inflammatory process encompassing both innate and adaptive immunity. Macrophages, the major immune cell population in atherosclerotic lesions, have been shown to play critical roles in all stages of atherosclerosis, including the initiation and progression of advanced atherosclerosis. Macrophages have emerged as a novel potential target for antiatherosclerosis therapy. In addition, the macrophage phenotype is greatly influenced by microenvironmental stimuli in the plaques and presents complex heterogeneity. This article reviews the functions of macrophages in different stages of atherosclerosis, as well as the phenotypes and functions of macrophage subsets. New treatment strategies based on macrophage-related inflammation are also discussed.
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http://dx.doi.org/10.1155/2019/4354786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914912PMC
May 2020

Correlation Between Endorectal Ultrasound and Magnetic Resonance Imaging for Predicting the Circumferential Resection Margin in Patients With Mid-Low Rectal Cancer Without Preoperative Chemoradiotherapy.

J Ultrasound Med 2020 Mar 16;39(3):569-577. Epub 2019 Oct 16.

Department of Ultrasound, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Objectives: To assess the correlation between endorectal ultrasound (ERUS) and magnetic resonance imaging (MRI) in predicting the circumferential resection margin (CRM) status of patients with mid-low rectal cancer without preoperative chemoradiotherapy.

Methods: Twenty patients with rectal cancer who did not receive preoperative chemoradiotherapy and underwent ERUS and MRI examinations before total mesorectal excision from May 2018 to April 2019 were included in this study. The patient and tumor characteristics, lymph nodes, tumor stages, ERUS and MRI predictors of the CRM status, and postoperative pathologic results were recorded. The closest distance between the deepest portion of lesion invasion and the mesorectal fascia was independently measured on MRI and ERUS images by 2 observers. The observers were blinded to the pathologic results. Measurements from ERUS and MRI were compared.

Results: The mean distance between the distal edge of the lesion and the anal verge was 5.7 cm (range, 3.1-8.1 cm). The ERUS and pathologic evaluations of CRM involvement were consistent in 90% of the cases. The MRI and pathologic evaluations of CRM involvement were concordant in 95% of the cases. The Cohen κ coefficient of ERUS and MRI was 0.608 (P = .007). The correlation coefficient of ERUS and MRI for assessing the closest distance from the edge of cancer invasion to the mesorectal fascia was 0.99 (P = .0005).

Conclusions: Endorectal ultrasound and MRI assessments of the preoperative CRM status appear to be highly consistent. Endorectal ultrasound can be used as a complementary tool with MRI to predict the CRM status of patients with mid-low rectal cancer without preoperative chemoradiotherapy.
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http://dx.doi.org/10.1002/jum.15135DOI Listing
March 2020

Cr(VI) Adsorption on Engineered Iron Oxide Nanoparticles: Exploring Complexation Processes and Water Chemistry.

Environ Sci Technol 2019 Oct 26;53(20):11913-11921. Epub 2019 Sep 26.

Department of Energy, Environmental and Chemical Engineering , Washington University in St. Louis , St. Louis , Missouri 63130 , United States.

Surface-functionalized magnetic nanoparticles are promising adsorbents due to their large surface areas and ease of separation after contaminant removal. In this work, the affinity of Cr(VI) adsorption to 8 nm surface-functionalized superparamagnetic magnetite nanoparticles was determined for surface coatings with amine (trimethyloctadecylammonium bromide, CTAB) and carboxyl (stearic acid, SA) functional groups. Cr(VI) adsorbed more strongly to the CTAB-coated nanoparticles than to the SA-coated materials due to electrostatic interactions between positively charged CTAB and anionic Cr(VI) species. The adsorption of Cr(VI) by CTAB- and SA-coated nanoparticles increased with decreasing pH (4.5-10), which could be simulated by a surface complexation model. Cr(VI) removal performance by the nanocomposite was evaluated for two realistic drinking water compositions. The co-occurrence of divalent cations (Ca and Mg) and Cr(VI) resulted in decreased Cr(VI) adsorption as particles were destabilized, leading to the aggregation and lower effective surface area, confirming the importance of the overall water composition on the performance of novel engineered nanomaterials for water treatment applications.
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http://dx.doi.org/10.1021/acs.est.9b03796DOI Listing
October 2019