Publications by authors named "Wenli Liu"

191 Publications

First report of norovirus sequences isolated from raccoon dogs in mainland China.

Virus Res 2021 Aug 13;305:198546. Epub 2021 Aug 13.

College of Life Science and Technology, Beijing University of Chemical Technology, China. Electronic address:

Noroviruses can infect humans and a wide variety of other mammalian hosts, causing varying degrees of diarrhea. In this study, two novel norovirus genomes were identified for the first time in farmed raccoon dogs, designated as raccoon dog noroviruses BUCT-K1 and BUCT-K4. Neither the farmers nor the raccoon dogs had symptoms (e.g., diarrhea) at the time of sample collection. We collected 14 stool samples from two farms, and 85.7% (12/14) of the samples were norovirus positive by RT-PCR. The two norovirus genomes have the highest identity to Dog/Z7/19/CH, suggesting that the norovirus might have been transmitted from dogs to raccoon dogs. Genomic and evolutionary analyses indicated that different directions of evolution occurred following the spread of the norovirus to the raccoon dogs. This study has increased knowledge of norovirus-infected animal species and has provided additional information on the norovirus family.
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http://dx.doi.org/10.1016/j.virusres.2021.198546DOI Listing
August 2021

spp. Omp25 Promotes Proteasome-Mediated cGAS Degradation to Attenuate IFN-β Production.

Front Microbiol 2021 29;12:702881. Epub 2021 Jul 29.

College of Veterinary Medicine, Northwest A&F University, Yangling, China.

Type I interferons (IFN), a family of cytokines widely expressed in various tissues, play important roles in anti-infection immunity. Nevertheless, it is not known whether spp. could interfere with IFN-I production induced by other pathogens. This study investigated the regulatory roles of outer membrane protein (Omp)25 on the IFN-I signaling pathway and found that Omp25 inhibited the production of IFN-β and its downstream IFN-stimulated genes induced by various DNA viruses or IFN-stimulatory DNA in human, murine, porcine, bovine, and ovine monocyte/macrophages or peripheral blood mononuclear cells. Omp25 suppressed the phosphorylation of stimulator of IFN genes (STINGs) and IFN regulatory factor 3 and nuclear translocation of phosphorylated IFN regulatory factor 3 in pseudorabies virus- or herpes simplex virus-1-infected murine, human, or porcine macrophages. Furthermore, we found that Omp25 promoted cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) degradation the proteasome-dependent pathway, resulting in a decreased cyclic guanosine monophosphate-adenosine monophosphate production and downstream signaling activation upon DNA virus infection or IFN-stimulatory DNA stimulation. Mapping the predominant function domain of Omp25 showed that the amino acids 161 to 184 of Omp25 were required for Omp25-induced cGAS degradation, among which five amino acid residues (R176, Y179, R180, Y181, and Y184) were required for the inhibitory effect of Omp25 on IFN-β induction. Altogether, our results demonstrated that Omp25 inhibits cGAS STING signaling pathway-induced IFN-β facilitating the ubiquitin-proteasome-dependent degradation of cGAS in various mammalian monocyte/macrophages.
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http://dx.doi.org/10.3389/fmicb.2021.702881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358459PMC
July 2021

Association between cytochrome P450 2C19 polymorphism and clinical outcomes in clopidogrel-treated Uygur population with acute coronary syndrome: a retrospective study.

BMC Cardiovasc Disord 2021 08 12;21(1):391. Epub 2021 Aug 12.

Department of Pharmacy, People's Hospital of Xinjiang Uygur Autonomous Region, No, 91 Tianchi Road, Tianshan District, Urumqi, 830001, Xinjiang, China.

Background: Acute coronary syndrome (ACS) has become a vital disease with high mortality in the Uygur populations. Clopidogrel plays an important role in reducing the risk of recurrent cardiovascular events after ACS; however, it is a prodrug that requires biotransformation by cytochrome P450 (CYP450).

Objectives: To determine the effect of genetic polymorphisms in CYP2C19*2, *3, and *17, and along with clinical, demographic factors, on variation in response to clinical outcomes in Uygur patients.

Methods: A total of 351 patients with ACS were treated with clopidogrel and aspirin for at least 12 months; we recorded major adverse cardiovascular events (MACE) or bleeding within 1 year. Multivariable logistic regression analyses were carried out to identify factors associated with MACE or bleeding.

Results: We analyze risk factors include age, BMI (body mass index), smoking, alcohol intake, NSTEMI (non-ST-segment elevation myocardial infarction), hypertension, dyslipidemia, concomitant medication, CYP2C19*2 carriers, CYP2C19*17 carriers and metabolizer phenotype. CYP2C19*2 carriers had an odds of having MACE of 2.51 (95% CI: 1.534-4.09) compared with noncarriers (P < .001). However, no factors were significantly associated with bleeding (P > 0.05).

Conclusion: The CYP2C19*2 gene polymorphism contributes to the risk of MACE in dual clopidogrel-treated Uygur population with ACS with or without PCI (percutaneous coronary intervention). These data may provide valuable insights into the genetic polymorphisms affecting clopidogrel metabolism among minority groups in China.
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http://dx.doi.org/10.1186/s12872-021-02201-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359105PMC
August 2021

A tomato LATERAL ORGAN BOUNDARIES transcription factor, , predominantly regulates cell wall and softening components of ripening.

Proc Natl Acad Sci U S A 2021 Aug;118(33)

Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853;

Fruit softening is a key component of the irreversible ripening program, contributing to the palatability necessary for frugivore-mediated seed dispersal. The underlying textural changes are complex and result from cell wall remodeling and changes in both cell adhesion and turgor. While a number of transcription factors (TFs) that regulate ripening have been identified, these affect most canonical ripening-related physiological processes. Here, we show that a tomato fruit ripening-specific () TF, , up-regulates a suite of cell wall-associated genes during late maturation and ripening of locule and pericarp tissues. repression in transgenic fruit impedes softening, while overexpression throughout the plant under the direction of the 35s promoter confers precocious induction of cell wall gene expression and premature softening. Transcript and protein levels of the wall-loosening protein EXPANSIN1 () are strongly suppressed in RNA interference lines, while is induced in -overexpressing transgenic leaves and fruit. In contrast to the role of ethylene and previously characterized ripening TFs, which are comprehensive facilitators of ripening phenomena including softening, participates in a regulatory subcircuit predominant to cell wall dynamics and softening.
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http://dx.doi.org/10.1073/pnas.2102486118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379924PMC
August 2021

Emotion matters: Face ensemble perception is affected by emotional states.

Psychon Bull Rev 2021 Jul 29. Epub 2021 Jul 29.

Department of Applied Psychology, College of Education, Hunan Agricultural University, Changsha, Hunan Province, 410128, People's Republic of China.

Ensemble coding is the ability of the visual system to extract a summary statistic from a set of stimuli. For example, observers often spontaneously extract an average face identity from a set of faces. Ensemble coding is known to operate in the frame of a distributed/global attention model. Because both attention and holistic processing are modulated by emotion - where positive emotions broaden the scope of attention and facilitate global processing, whereas negative emotions narrow the scope of attention and promote local processing - the current research explored whether emotional states could affect visual averaging of multiple face identities. Participants completed an ensemble-coding task before and after their emotion was induced via film clips. In the ensemble-coding task, a set of four face identities was shown briefly, followed by a probe face. Participants judged whether the probe face was presented in the preceding set. Evidence for ensemble coding was indexed by responses that treated an average face of the preceding set as a member of that set. The results showed that the tendency to choose this average was modulated by emotional states. Visual averaging increased after seeing positive film clips, but decreased after seeing negative film clips. These results support Frederickson's broaden-and-built theory, and extended its application to ensemble perception.
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http://dx.doi.org/10.3758/s13423-021-01987-wDOI Listing
July 2021

Zwint facilitates melanoma progression by promoting c-Myc expression.

Exp Ther Med 2021 Aug 2;22(2):818. Epub 2021 Jun 2.

Department of Dermatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.

ZW10 interactor (Zwint) is upregulated in various types of tumors and exerts a carcinogenic effect. However, little is known about the expression profile, function and molecular mechanisms of action of Zwint in melanoma. Therefore, the aim of the present study was to investigate the expression levels of Zwint in melanoma cell lines and tissues. It was revealed that Zwint was highly expressed in melanoma samples. Functional experiments indicated that Zwint knockdown suppressed the proliferation and migration of A375 melanoma cells. Further mechanistic studies demonstrated that Zwint knockdown decreased the protein expression levels of c-Myc, MMP-2, Slug, mTOR, phosphorylated (p)-mTOR, p-p38 and fibronectin, while it increased the protein expression levels of E-cadherin and MMP-9. Among these genes, c-Myc, MMP-2 and Slug were overexpressed to investigate their effects on cell proliferation following Zwint knockdown. The results demonstrated that overexpression of c-Myc, but not MMP-2 or Slug, rescued the effects of Zwint knockdown on melanoma cell proliferation and migration. Taken together, the results of the present study suggested that Zwint may act as an oncogene in melanoma by regulating c-Myc expression.
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http://dx.doi.org/10.3892/etm.2021.10250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193213PMC
August 2021

Cyst(e)ine in nutrition formulation promotes colon cancer growth and chemoresistance by activating mTORC1 and scavenging ROS.

Signal Transduct Target Ther 2021 May 28;6(1):188. Epub 2021 May 28.

Department of the Second Medical Oncology, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China.

Weight loss and cachexia are common problems in colorectal cancer patients; thus, parenteral and enteral nutrition support play important roles in cancer care. However, the impact of nonessential amino acid components of nutritional intake on cancer progression has not been fully studied. In this study, we discovered that gastrointestinal cancer patients who received cysteine as part of the parenteral nutrition had shorter overall survival (P < 0.001) than those who did not. Cystine indeed robustly promotes colon cancer cell growth in vitro and in immunodeficient mice, predominately by inhibiting SESN2 transcription via the GCN2-ATF4 axis, resulting in mTORC1 activation. mTORC1 inhibitors Rapamycin and Everolimus block cystine-induced cancer cell proliferation. In addition, cystine confers resistance to oxaliplatin and irinotecan chemotherapy by quenching chemotherapy-induced reactive oxygen species via synthesizing glutathione. We demonstrated that dietary deprivation of cystine suppressed colon cancer xenograft growth without weight loss in mice and boosted the antitumor effect of oxaliplatin. These findings indicate that cyst(e)ine, as part of supplemental nutrition, plays an important role in colorectal cancer and manipulation of cyst(e)ine content in nutritional formulations may optimize colorectal cancer patient survival.
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http://dx.doi.org/10.1038/s41392-021-00581-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160199PMC
May 2021

Acupuncture for Cancer-Related Anorexia: a Review of the Current Evidence.

Curr Oncol Rep 2021 May 5;23(7):82. Epub 2021 May 5.

Department of Palliative, Rehabilitation and Integrative Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX, 77030, USA.

Purpose Of Review: Loss of appetite/anorexia is extremely common among cancer patients, affecting as many as half of newly diagnosed patients and 70% of patients with advanced disease. Effective management of this disabling symptom of cancer remains a major challenge in the field of oncology. We conducted a systematic review of the current evidence on acupuncture and/or moxibustion as an intervention for cancer-related anorexia.

Recent Findings: Acupuncture, as a part of traditional Chinese medicine practice, has demonstrated effectiveness in managing many cancer- and treatment-related symptoms, especially chemotherapy-induced or postoperative nausea. However, the efficacy of acupuncture in treating cancer-related anorexia/loss of appetite is not clear. The current level of evidence is insufficient to make a definitive conclusion on the benefit of acupuncture/moxibustion for treating chronic cancer-related anorexia/appetite problems. Future large randomized controlled trials of high methodological quality are needed.
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http://dx.doi.org/10.1007/s11912-021-01067-1DOI Listing
May 2021

miR-139-5p Loss-Mediated WTAP Activation Contributes to Hepatocellular Carcinoma Progression by Promoting the Epithelial to Mesenchymal Transition.

Front Oncol 2021 15;11:611544. Epub 2021 Apr 15.

Clinical Laboratory Diagnostics, Medical Technology College, Beihua University, Jilin, China.

Hepatocellular carcinoma (HCC) is a primary aggressive gastrointestinal neoplasm that affects patients worldwide. It has been shown that Wilms' tumor 1-associating protein (WTAP) is frequently upregulated in various cancers. However, the potential role of WTAP in HCC remains largely unknown. The expression levels of WTAP in human HCC tissues were determined by the western blotting and immunohistochemical (IHC) staining. A correlation between the WTAP expression, clinicopathological features, and the HCC prognosis was analyzed. The WTAP expression was silenced by short hairpin RNA (shRNA), and effects of the knockdown of WTAP on the proliferation and invasion of HCC cells were assessed. The microRNAs (miRNAs) involved in the regulation of the WTAP expression were identified by a bioinformatics analysis and further confirmed by assays. The expression levels of WTAP in liver cancer tissues were significantly elevated and compared with those in the adjacent normal tissues and significantly correlated with the clinical stage and prognosis in patients with HCC. Further investigation revealed that the knockdown of WTAP drastically suppressed HCC cell proliferation and invasion abilities. Luciferase reporter assay and validation experiments confirmed that WTAP was a direct target of miR-139-5p. Moreover, the overexpression of WTAP could partly abolish the inhibitory effects of miR-139-5p on the HCC cell growth and invasion. Mechanistically, we revealed that the miR-139-5p/WTAP axis regulated the HCC progression by controlling the epithelial to mesenchymal transition (EMT). In summary, the results indicate that WTAP is a potential oncogene in HCC and miR-139-5p negatively regulates the WTAP expression. MiR-139-5p/WTAP can be utilized as a potential therapeutic target for HCC.
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http://dx.doi.org/10.3389/fonc.2021.611544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083052PMC
April 2021

Experimental Investigation of the Attachment Performance between Coal Particle and Bubble.

ACS Omega 2021 Mar 18;6(12):7979-7987. Epub 2021 Mar 18.

School of Chemical and Environmental Engineering, China University of Mining & Technology (Beijing), Beijing 100083, China.

Attachment behavior is a key component of flotation and has a decisive influence on flotation performance, and the experiment research on the attachment between mineral particles and bubbles still needs further research. In this work, a particle-bubble attachment apparatus and multiple target tracking software were developed. Coal particles were used as the subjects, and the effect of particle properties on the attachment performance was studied from the perspective of the particle group. The particle-bubble attachment experiments indicated that the collision position had an effect on the attachment efficiency, and the attachment efficiency decreased with an increase in the collision angle. The efficiency-weighted attachment angle was proposed to quantitatively describe the attachment performance of coal samples. The efficiency-weighted attachment angle of low-density coal samples was greater than that of high-density coal samples. For particles with different sizes, the efficiency-weighted attachment angle of fine particles was greater than that of coarse particles. Furthermore, SDS weakened the attachment performance between coal particles and bubbles via adsorption on the bubble, and the efficiency-weighted attachment angle decreased as the concentration of the SDS solution increased. CTAB adsorbed on coal particles and bubbles, and the efficiency-weighted attachment angle first increased and then decreased with increasing CTAB concentration.
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http://dx.doi.org/10.1021/acsomega.0c04093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014942PMC
March 2021

Circ_0084043 promotes cell proliferation and glycolysis but blocks cell apoptosis in melanoma via circ_0084043-miR-31-KLF3 axis.

Open Life Sci 2020 22;15(1):774-786. Epub 2020 Oct 22.

Department of Oncology, Affiliated Nanhua Hospital, University of South China, No. 336, Dongfang South Road, Zhuhui District, Hengyang 421000, Hunan, China.

Melanoma is an aggressive malignant tumor. The crucial role of circular RNAs has been documented in many types of cancer, including melanoma. The objective of this study was to uncover the function of circ_0084043 in the biological process of melanoma and associated mechanism of action. The expression of circ_0084043, miR-31, and Krüppel-like factor 3 (KLF3) was determined by qRT-PCR. Cell proliferation and apoptosis were monitored by the MTT assay and flow cytometry assay, respectively. The progression of glycolysis was evaluated according to the levels of glucose consumption, lactate production, and ATP concentration using appropriate detection kits. The relationship between miR-31 and circ_0084043 or KLF3 was predicted by the bioinformatics tool and ascertained by the dual-luciferase reporter assay. The protein levels of KLF3 and glucose transporter 1 (Glut1) were quantified by western blot. A xenograft model was established to ascertain the role of circ_0084043 . As a result, circ_0084043 expression was reinforced in melanoma tissues and cells. Circ_0084043 knockdown inhibited cell proliferation, induced cell apoptosis, and restrained glycolysis. MiR-31 was a target of circ_0084043, and miR-31 deficiency reversed the role of circ_0084043 knockdown. KLF3 was targeted by miR-31, and KLF3 upregulation abolished the effects of miR-31 enrichment. Moreover, circ_0084043 knockdown impeded tumor growth and suppressed the level of Glut1 by modulating miR-31 and KLF3. Circ_0084043 promoted cell proliferation and glycolysis, and blocked apoptosis through the circ_0084043-miR-31-KLF3 regulatory axis in melanoma.
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http://dx.doi.org/10.1515/biol-2020-0071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747509PMC
October 2020

Bone marrow stem cells secretome accelerates simulated birth trauma-induced stress urinary incontinence recovery in rats.

Aging (Albany NY) 2021 03 31;13(7):10517-10534. Epub 2021 Mar 31.

Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.

Stress urinary incontinence (SUI) is defined as involuntary urine leakage during physical activities that increase the intra-abdominal pressure on the bladder. We studied bone marrow stem cell (BMSC) secretome-induced activation of anterior vaginal wall (AVW) fibroblasts and its ability to accelerate SUI recovery following vaginal distention (VD) in a rat model of birth trauma using BMSC-conditioned medium (BMSC-CM) and concentrated conditioned medium (CCM). BMSC-CM enhanced the proliferation, migration, and collagen synthesizing abilities of fibroblasts. Differentially expressed genes in BMSC-CM-induced fibroblasts were mainly enriched for cell adhesion, extracellular fibril organization and angiogenesis. Treatment with the JAK2 inhibitor AG490 reversed BMSC-CM-induced activation of the JAK2/STAT4 pathway. Periurethral injection with BMSC-CCM markedly enhanced the abdominal leak point pressure (LPP) in rats after VD. Histological analysis revealed increased numbers of fibroblasts, improved collagen fibers arrangement and elevated collagens content in the AVW of rats receiving BMSC-CCM. These findings suggest the BMSC secretome activates AVW fibroblasts and contributes to the functional and anatomic recovery of simulated birth trauma-induced SUI in rats.
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http://dx.doi.org/10.18632/aging.202812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064190PMC
March 2021

Alterations in the human oral and gut microbiomes and lipidomics in COVID-19.

Gut 2021 07 31;70(7):1253-1265. Epub 2021 Mar 31.

State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China

Objective: To characterise the oral microbiome, gut microbiome and serum lipid profiles in patients with active COVID-19 and recovered patients; evaluate the potential of the microbiome as a non-invasive biomarker for COVID-19; and explore correlations between the microbiome and lipid profile.

Design: We collected and sequenced 392 tongue-coating samples, 172 faecal samples and 155 serum samples from Central China and East China. We characterised microbiome and lipid molecules, constructed microbial classifiers in discovery cohort and verified their diagnostic potential in 74 confirmed patients (CPs) from East China and 37 suspected patients (SPs) with IgG positivity.

Results: Oral and faecal microbial diversity was significantly decreased in CPs versus healthy controls (HCs). Compared with HCs, butyric acid-producing bacteria were decreased and lipopolysaccharide-producing bacteria were increased in CPs in oral cavity. The classifiers based on 8 optimal oral microbial markers (7 faecal microbial markers) achieved good diagnostic efficiency in different cohorts. Importantly, diagnostic efficacy reached 87.24% in the cross-regional cohort. Moreover, the classifiers successfully diagnosed SPs with IgG antibody positivity as CPs, and diagnostic efficacy reached 92.11% (98.01% of faecal microbiome). Compared with CPs, 47 lipid molecules, including sphingomyelin (SM)(d40:4), SM(d38:5) and monoglyceride(33:5), were depleted, and 122 lipid molecules, including phosphatidylcholine(36:4p), phosphatidylethanolamine (PE)(16:0p/20:5) and diglyceride(20:1/18:2), were enriched in confirmed patients recovery.

Conclusion: This study is the first to characterise the oral microbiome in COVID-19, and oral microbiomes and lipid alterations in recovered patients, to explore their correlations and to report the successful establishment and validation of a diagnostic model for COVID-19.
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http://dx.doi.org/10.1136/gutjnl-2020-323826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042598PMC
July 2021

The landscape and prognostic value of immune characteristics in uterine corpus endometrial cancer.

Biosci Rep 2021 04;41(4)

Department of Gynecologic Oncology, The First Affiliated Hospital of Henan Polytechnic University, Jiaozuo 454000, China.

In the present study, we explored the clinical and immunological characteristics of 575 uterine corpus endometrial carcinoma (UCEC) samples obtained from The Cancer Genome Atlas (TCGA) using the ESTIMATE and CIBERSORT algorithms. First, Kaplan-Meier and univariate Cox regression analyses indicated that the immune cell score was a prognostic factor for overall survival (OS) and recurrence-free survival (RFS). Multivariate Cox regression analysis further revealed that the immune cell score was an independent prognostic factor for UCEC patients. Second, we investigated the correlation between the infiltration levels of 22 types of immune cells and the immune score. Survival analysis based on the 22 immune cell types showed that higher levels of regulatory T cell, activated NK cell, and follicular helper T-cell infiltration were associated with longer OS, while higher levels of CD8+ T cell and naive B-cell infiltration were associated with longer RFS. Next, we performed differential expression and prognosis analyses on 1534 immune-related genes and selected five from 14 candidate genes to construct a prognostic prediction model. The area under the receiver-operating characteristic (ROC) curve (AUC) for 3- and 5-year survival were 0.711 and 0.728, respectively. Further validation using a stage I-II subgroup showed similar results, presenting AUC values for 3- and five-year survival of 0.677 and 0.692, respectively. Taken together, the present study provides not only a deeper understanding of the relationship between UCEC and the immune landscape but also guidance for the future development of UCEC immunotherapy.
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http://dx.doi.org/10.1042/BSR20202321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062954PMC
April 2021

The AIM2 inflammasome exacerbates atherosclerosis in clonal haematopoiesis.

Nature 2021 04 17;592(7853):296-301. Epub 2021 Mar 17.

Division of Molecular Medicine, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA.

Clonal haematopoiesis, which is highly prevalent in older individuals, arises from somatic mutations that endow a proliferative advantage to haematopoietic cells. Clonal haematopoiesis increases the risk of myocardial infarction and stroke independently of traditional risk factors. Among the common genetic variants that give rise to clonal haematopoiesis, the JAK2 (JAK2) mutation, which increases JAK-STAT signalling, occurs at a younger age and imparts the strongest risk of premature coronary heart disease. Here we show increased proliferation of macrophages and prominent formation of necrotic cores in atherosclerotic lesions in mice that express Jak2 selectively in macrophages, and in chimeric mice that model clonal haematopoiesis. Deletion of the essential inflammasome components caspase 1 and 11, or of the pyroptosis executioner gasdermin D, reversed these adverse changes. Jak2 lesions showed increased expression of AIM2, oxidative DNA damage and DNA replication stress, and Aim2 deficiency reduced atherosclerosis. Single-cell RNA sequencing analysis of Jak2 lesions revealed a landscape that was enriched for inflammatory myeloid cells, which were suppressed by deletion of Gsdmd. Inhibition of the inflammasome product interleukin-1β reduced macrophage proliferation and necrotic formation while increasing the thickness of fibrous caps, indicating that it stabilized plaques. Our findings suggest that increased proliferation and glycolytic metabolism in Jak2 macrophages lead to DNA replication stress and activation of the AIM2 inflammasome, thereby aggravating atherosclerosis. Precise application of therapies that target interleukin-1β or specific inflammasomes according to clonal haematopoiesis status could substantially reduce cardiovascular risk.
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http://dx.doi.org/10.1038/s41586-021-03341-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038646PMC
April 2021

Soil and ambient air mercury as an indicator of coal-fired power plant emissions: a case study in North China.

Environ Sci Pollut Res Int 2021 Feb 26. Epub 2021 Feb 26.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, China.

Coal-fired power plants (CFPPs) are an important anthropogenic mercury (Hg) source in China, and it is crucial to understand the environmental impacts of this detrimental element emitted from this source. In the present study, field experiments were conducted for measuring Hg in ambient atmosphere and upland agricultural soils within a radius of 10 km surrounding a large scale coal-fired power plant (1550 MW) in Tangshan, Hebei province. Short-term (20 min) average of gaseous elemental mercury (GEM or Hg) in ambient air varying from 1.5 to 9.0 ng/m and total Hg (THg) in surface agricultural soil (0-20 cm) varying from 9.2 to 43.5 μg/kg at different sites were observed. THg in two soil cores decreased with depth, with concentrations being 2-2.5 times higher in the surface layer than that in the deep layer (50-60 cm), indicating the possibility of the atmospheric input of Hg. Based on the information of the total atmospheric Hg emission since this CFPP's operation in 1970s and the increased THg in nearby soils, it was estimated that about 3.9% discharged Hg has accumulated in the nearby agricultural soils. The low retention rate of the total emitted Hg by soils is a result of high proportion of Hg (79.5%) in stack gas emission and potential loss of Hg from soil surface reemission. The positive shifting (~ 0.5‰) of Hg isotopic signature (δHg) from deep soil to surface soil reflected Hg deposition from nearby CFPP emissions that are featured with much heavier Hg isotopic signatures inherited from feed coal (δHg: -0.50‰) and different combustion products (δHg: -0.95 to 3.71‰) compared with that in deep soil layer (δHg: ca -1.50‰). Overall, this study demonstrated that this CFPP has a slight but distinguishable effect on the elevation of ambient GEM and agricultural soil THg in the local environment.
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http://dx.doi.org/10.1007/s11356-021-12842-9DOI Listing
February 2021

Highly paralleled emulsion droplets for efficient isolation, amplification, and screening of cancer biomarker binding phages.

Lab Chip 2021 03 8;21(6):1175-1184. Epub 2021 Feb 8.

The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, Department of Chemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Based on the linkage of genotype and phenotype, display technology has been widely used to generate specific ligands for profiling, imaging, diagnosis and therapy applications. However, due to the lack of effective monoclonal manipulation and affinity evaluation methods, traditional display technology has to undergo tedious steps of selection, clone isolation, amplification, sequencing, synthesis and characterization to obtain the binding sequences. To directly acquire high-affinity clones, we propose a double monoclonal display approach (dm-Display) for peptide screening based on highly paralleled monoclonal manipulation in emulsion droplets. dm-Display can monoclonally link the genotype, phenotype and affinity to realize integrated monoclonal separation, amplification, recognition and staining in one droplet so that discrete high-affinity clones can be quickly extracted. Monoclonal manipulations highly-parallelly occur in millions of droplets so that molecular screening of a highly diverse phage library is achieved. We have screened specific peptide ligands against CD71 and GPC1, proving the feasibility and generality of dm-Display. As a highly efficient ligand screening platform, dm-Display will promote the further development of molecular screening.
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http://dx.doi.org/10.1039/d0lc01146kDOI Listing
March 2021

Long-term urbanization impacts the eastern golden frog () in Shanghai City: Demographic history, genetic structure, and implications for amphibian conservation in intensively urbanizing environments.

Evol Appl 2021 Jan 7;14(1):117-135. Epub 2020 Nov 7.

School of Life Sciences East China Normal University Shanghai China.

Understanding the mechanisms of how urbanization influences the evolution of native species is vital for urban wildlife ecology and conservation in the Anthropocene. With thousands of years of agriculture-dominated historical urbanization followed by 40 years of intensive and rapid urbanization, Shanghai provides an ideal environment to study how the two-stage urbanization process influences the evolution of indigenous wildlife, especially of anuran species. Therefore, in this study, we used mitochondrial gene, microsatellite (SSR), and single nucleotide polymorphism (SNP) data to evaluate the demographic history and genetic structure of the eastern golden frog (), by sampling 407 individuals from 15 local populations across Shanghai, China. All local populations experienced bottlenecks during historical urbanization, while the local populations in urban areas maintained comparable contemporary effective population sizes ( ) and genetic diversity with suburban and rural populations. Nevertheless, the rapid modern urbanization has already imposed significant negative effects to the integrity of populations. The 15 local populations were differentiated into eight genetic clusters, showing a spatial distribution pattern consistent with the current urbanization gradient and island-mainland geography. Although moderate gene flow still occurred from the rural peripheral cluster to urban and suburban clusters, population fragmentation was more serious in the urban and suburban populations, where higher urbanization levels within 2-km radius areas showed significant negative relationships to the and genetic diversity of local populations. Therefore, to protect urban wildlife with limited dispersal ability, improving conditions in fragmented habitat remnants might be most essential for local populations living in more urbanized areas. Meanwhile, we highlight the need to preserve large unfragmented rural habitats and to construct corridor networks to connect discrete urban habitat remnants for the long-term wildlife conservation in intensively urbanizing environments.
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http://dx.doi.org/10.1111/eva.13156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819575PMC
January 2021

Transcriptome analysis of cepharanthine against a SARS-CoV-2-related coronavirus.

Brief Bioinform 2021 03;22(2):1378-1386

Department of Rheumatology and Immunology, West China Hospital, Sichuan University.

Antiviral therapies targeting the pandemic coronavirus disease 2019 (COVID-19) are urgently required. We studied an already-approved botanical drug cepharanthine (CEP) in a cell culture model of GX_P2V, a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-related virus. RNA-sequencing results showed the virus perturbed the expression of multiple genes including those associated with cellular stress responses such as endoplasmic reticulum (ER) stress and heat shock factor 1 (HSF1)-mediated heat shock response, of which heat shock response-related genes and pathways were at the core. CEP was potent to reverse most dysregulated genes and pathways in infected cells including ER stress/unfolded protein response and HSF1-mediated heat shock response. Additionally, single-cell transcriptomes also confirmed that genes of cellular stress responses and autophagy pathways were enriched in several peripheral blood mononuclear cells populations from COVID-19 patients. In summary, this study uncovered the transcriptome of a SARS-CoV-2-related coronavirus infection model and anti-viral activities of CEP, providing evidence for CEP as a promising therapeutic option for SARS-CoV-2 infection.
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http://dx.doi.org/10.1093/bib/bbaa387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929461PMC
March 2021

ZFPM2-AS1 facilitates cell proliferation and migration in cutaneous malignant melanoma through modulating miR-650/NOTCH1 signaling.

Dermatol Ther 2021 03 23;34(2):e14751. Epub 2021 Jan 23.

Department of Dermatology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Aberrant expression of long non-coding RNA (lncRNA) zinc finger protein, FOG family member 2 antisense RNA 1 (ZFPM2-AS1) has been identified in many tumors, but its role in cutaneous malignant melanoma remains largely obscure. Our present study was intended to unveil the role and potential mechanism of ZFPM2-AS1 in cutaneous malignant melanoma. RT-qPCR was utilized to analyze ZFPM2-AS1 expression in cutaneous malignant melanoma cells. Cell counting kit-8 (CCK-8), colony formation, flow cytometry, and transwell analyses were utilized to assess ZFPM2-AS1 function on cell proliferation, apoptosis, and migration. Luciferase reporter, RNA immunoprecipitation, and RNA-pull down assays were applied to probe the regulatory mechanism of ZFPM2-AS1 in cutaneous malignant melanoma cells. Up-regulation of ZFPM2-AS1 was discovered in cutaneous malignant melanoma cells. ZFPM2-AS1 deletion restrained cell proliferation, migration, and elevated cell apoptosis in cutaneous malignant melanoma. ZFPM2-AS1 regulated notch receptor 1 (NOTCH1) to activate the NOTCH pathway. ZFPM2-AS1 acted as a competing endogenous RNA (ceRNA) to affect NOTCH1 expression via sponging miR-650. Collectively, ZFPM2-AS1 exerted an oncogenic role in cutaneous malignant melanoma progression via targeting miR-650/NOTCH1 signaling. Our study might offer a novel sight for cutaneous malignant melanoma treatment.
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http://dx.doi.org/10.1111/dth.14751DOI Listing
March 2021

Overcoming Barriers for Clinical Research of Acupuncture.

Med Acupunct 2020 Dec 16;32(6):348-351. Epub 2020 Dec 16.

Department of Palliative, Rehabilitation & Integrative Medicine, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Acupuncture is increasingly being used in the management of a wide range of symptoms. Despite decades of acupuncture research, a significant gap remains in translating evidence into specific and clear clinical guidelines for acupuncture practice. In this article, the authors discuss the gap between acupuncture research and clinical practice and exploring options to overcome the limitations of current clinical research of acupuncture.
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http://dx.doi.org/10.1089/acu.2020.1480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755839PMC
December 2020

Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis.

Front Cell Dev Biol 2020 25;8:566494. Epub 2020 Nov 25.

Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Glycogen metabolism plays a key role in tumorigenesis. High expression levels of glycogen phosphorylase B (PYGB) were reported in several cancers and might be served as a prognostic biomarker for cancer from precancerous lesions. Previous studies indicated the high expression of PYGB in hepatocellular carcinoma (HCC) tissues. However, the detailed roles of PYGB in HCC, as well as the regulatory mechanisms, are still unclear. In this study, we confirmed that PYGB was overexpressed in HCC tissues. PYGB overexpression was significantly associated with an aggressive tumor phenotype and poor prognosis of HCC patients. Functionally, PYGB knockdown suppressed HCC cell proliferation, migration and invasion , as well as tumorigenesis and metastasis . Bioinformatics analysis indicated that PYGB overexpression might enhance epithelial to mesenchymal transition (EMT) in HCC. Moreover, miR-101-3p was identified to post-transcriptionally inhibit the expression of PYGB via binding to 3'-UTR of PYGB. Overexpression of PYGB antagonized the regulatory effect of miR-101-3p on cell proliferation, migration and invasion in HCC cells. In summary, our results suggest that miR-101-3p/PYGB axis has an important role in HCC and PYGB could be served as a novel prognostic biomarker and therapeutic target for improving the prognosis of HCC patients.
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http://dx.doi.org/10.3389/fcell.2020.566494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723997PMC
November 2020

Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYC.

Sci Rep 2020 12 14;10(1):21924. Epub 2020 Dec 14.

Molecular and Clinical Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg. 10, Room 9N119, 9000 Rockville Pike, Bethesda, MD, 20892, USA.

Olfactomedin 4 (OLFM4) is expressed in normal prostate epithelial cells and immortalized normal human prostate epithelial cells (RWPE1), but the identity of OLFM4-expressing cells within these populations and OLFM4's physiological functions in these cells have not been elucidated. Using single-cell RNA sequencing analysis, we found here that OLFM4 was expressed in multiple stem/progenitor-like cell populations in both the normal prostate epithelium and RWPE1 cells and was frequently co-expressed with KRT13 and LY6D in RWPE1 cells. Functionally, OLFM4-knockout RWPE1 cells exhibited enhanced proliferation of the stem/progenitor-like cell population, shifts stem/progenitor-like cell division to favor symmetric division and differentiated into higher levels PSA expression cells in organoid assays compared with OLFM4-wild RWPE1 cells. Bulk-cell RNA sequencing analysis pinpointed that cMYC expression were enhanced in the OLFM4-knockout RWPE1 cells compared with OLFM4-wild cells. Molecular and signaling pathway studies revealed an increase in the WNT/APC/MYC signaling pathway gene signature, as well as that of MYC target genes that regulate multiple biological processes, in OLFM4-knockout RWPE1 cells. These findings indicated that OLFM4 is co-expressed with multiple stem/progenitor cell marker genes in prostate epithelial cells and acts as a novel mediator in prostate stem/progenitor cell proliferation and differentiation.
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http://dx.doi.org/10.1038/s41598-020-78774-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7736579PMC
December 2020

Dominant Loss Mechanisms of Whispering Gallery Mode RF-MEMS Resonators with Wide Frequency Coverage.

Sensors (Basel) 2020 Dec 8;20(24). Epub 2020 Dec 8.

Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.

This work investigates the dominant energy dissipations of the multi-frequency whispering gallery mode (WGM) resonators to provide an insight into the loss mechanisms of the devices. An extensive theory for each loss source was established and experimentally testified. The squeezed film damping (SFD) is a major loss for all the WGMs at atmosphere, which is distinguished from traditional bulk acoustic wave (BAW) resonators where the high-order modes suffer less from the air damping. In vacuum, the SFD is negligible, and the frequency-dependent Akhiezer damping (AKE) has significant effects on different order modes. For low-order WGMs, the AKE is limited, and the anchor loss behaves as the dominant loss. For high-order modes with an extended nodal region, the anchor loss is reduced, and the AKE determines the values. Substantial enhancements over four times and an excellent × product up to 6.36 × 10 at 7 K were achieved.
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http://dx.doi.org/10.3390/s20247017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764441PMC
December 2020

The Use of Mobile-technologies to Deliver Integrative Medicine During and Beyond the COVID-19 World Pandemic.

Glob Adv Health Med 2020 29;9:2164956120977437. Epub 2020 Nov 29.

Department of Palliative, Rehabilitation, and Integrative Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas.

COVID-19 Pandemic has catalyzed the use of tele-medicine technologies in health care, especially in cancer. The recent relaxation of guidelines has enabled rapid expansion of these platforms many have been seeking for years. We review the advantages and challenges of delivering integrative oncology care using telemedicine. The article concludes with recommendations for areas that need to be addressed so that future practice can consider a hybrid model.
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http://dx.doi.org/10.1177/2164956120977437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705776PMC
November 2020

Rapid droplet multiple displacement amplification based on the droplet regeneration strategy.

Anal Chim Acta 2021 Jan 19;1141:173-179. Epub 2020 Oct 19.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. Electronic address:

Multiple displacement amplification (MDA) is a popular whole genome amplification method for its simplicity and high coverage on whole genome DNA. Yet it suffers from uneven magnification and long reaction time. Here we present the cyclical concentration adjusting concept to speed up MDA and realize the droplet dilution steps with a novel droplet re-generation strategy. The increased initial concentration shortened inefficient time and the repeated high-speed period maintained the reaction efficient for long. This rapid droplet MDA method could save more than 1/2 of the total reaction time and exhibited higher accuracy than the conventional method. The cyclical concentration adjusting method alters nothing but the concentration of reactants in different periods of reaction. Therefore, it may be widely utilized to accelerating MDA-like time-consuming reactions without concerns of compatibility.
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http://dx.doi.org/10.1016/j.aca.2020.10.031DOI Listing
January 2021

Alterations of the Human Gut Microbiome in Chronic Kidney Disease.

Adv Sci (Weinh) 2020 Oct 2;7(20):2001936. Epub 2020 Sep 2.

State Key Laboratory for Diagnosis and Treatment of Infectious Disease National Clinical Research Center for Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China.

Gut microbiota make up the largest microecosystem in the human body and are closely related to chronic metabolic diseases. Herein, 520 fecal samples are collected from different regions of China, the gut microbiome in chronic kidney disease (CKD) is characterized, and CKD classifiers based on microbial markers are constructed. Compared with healthy controls (HC, = 210), gut microbial diversity is significantly decreased in CKD ( = 110), and the microbial community is remarkably distinguished from HC. Genera and are enriched, while and are reduced in CKD. Fifty predicted microbial functions including tryptophan and phenylalanine metabolisms increase, while 36 functions including arginine and proline metabolisms decrease in CKD. Notably, five optimal microbial markers are identified using the random forest model. The area under the curve (AUC) reaches 0.9887 in the discovery cohort and 0.9512 in the validation cohort (49 CKD vs 63 HC). Importantly, the AUC reaches 0.8986 in the extra diagnosis cohort from Hangzhou. Moreover, and are increased with CKD progression. Thirteen operational taxonomy units are correlated with six clinical indicators of CKD. In conclusion, this study comprehensively characterizes gut microbiome in non-dialysis CKD and demonstrates the potential of microbial markers as non-invasive diagnostic tools for CKD in different regions of China.
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http://dx.doi.org/10.1002/advs.202001936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578882PMC
October 2020

Hypomagnesemia and incidence of osteoradionecrosis in patients with head and neck cancers.

Head Neck 2021 02 23;43(2):613-621. Epub 2020 Oct 23.

Department of Head and Neck Surgery, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA.

Background: We aimed to determine whether hypomagnesemia predicts osteoradionecrosis development in patients with squamous cell carcinoma of the oropharynx and oral cavity who received platinum-based concurrent chemoradiation with or without induction therapy.

Methods: We reviewed data from patients with head and neck cancers who had undergone chemoradiation with weekly cisplatin/carboplatin between January 1, 2010 and December 31, 2014 at our institution. Pathologic features, laboratory test results, disease stage, and social histories were recorded. The association between hypomagnesemia and osteoradionecrosis was analyzed controlling for known confounding factors.

Results: Hypomagnesemia during cancer treatment was associated with osteoradionecrosis development (HR = 2.72, P = .037) independent of total radiation dose (HR = 1.07, P = .260) and smoking history (HR = 2.05, P = .056) among the patients who received platinum-based induction chemotherapy followed by concurrent chemoradiation.

Conclusions: Hypomagnesemia was predictive of the development of osteoradionecrosis in patients with cancers of the oropharynx and oral cavity receiving platinum-based induction followed by concurrent chemoradiation.
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http://dx.doi.org/10.1002/hed.26510DOI Listing
February 2021

Hypomagnesemia and survival in patients with head and neck cancers who received primary concurrent chemoradiation.

Cancer 2021 02 21;127(4):528-534. Epub 2020 Oct 21.

Department of Emergency Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas.

Background: Prior research has confirmed that persistent hypomagnesemia was predictive of shorter survival among patients with ovarian cancer who received carboplatin-based chemotherapy. In the current retrospective study, the authors examined the association between hypomagnesemia and survival in patients with head and neck cancer who received concurrent chemoradiation with weekly infusions of cisplatin and/or carboplatin.

Methods: Patients with head and neck cancers who had undergone chemoradiation with cisplatin and/or carboplatin between January 1, 2010, and December 31, 2014, were included. Patients were aged ≥18 years with pathology of squamous cell carcinoma of the larynx, oral cavity, or oropharynx who had received at least 30 fractions of radiotherapy with concurrent weekly cisplatin and/or carboplatin. Pathology features, laboratory results, Eastern Cooperative Oncology Group performance status, social histories, and survival were recorded. The association between hypomagnesemia and survival was analyzed controlling for known prognostic factors.

Results: The final cohort consisted of 439 patients with a median age of 59 years. A greater frequency of hypomagnesemia during the treatment course was found to be significantly associated with shorter survival (hazard ratio [HR], 1.13; P = .033) independent of age (HR, 1.65; P = .042), cancer site (nonoropharynx vs oropharynx: HR, 2.15 [P = .003]), Eastern Cooperative Oncology Group performance status (>1 vs ≤1: HR, 2.64 [P < .001]), and smoking history (smoker vs nonsmoker: HR, 1.88 [P = .012]). In addition, more severe hypomagnesemia was associated with shorter survival compared with the milder form.

Conclusions: The frequency and severity of hypomagnesemia during treatment are prognostic of survival for patients with head and neck cancers who are receiving concurrent chemoradiation with cisplatin and/or carboplatin. A prospective study is needed to investigate the impact of the prevention of hypomagnesemia on survival in this patient population.
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http://dx.doi.org/10.1002/cncr.33283DOI Listing
February 2021

Construction of Molecular Model and Adsorption of Collectors on Bulianta Coal.

Molecules 2020 Sep 3;25(17). Epub 2020 Sep 3.

School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China.

To study the effects of different oxygen functional groups on the quality of flotation clean low-rank coal, two kinds of collectors with different oxygen-containing functional groups, methyl laurate, and dodecanol, were selected and their flotation behaviors were investigated. The Bulianta coal was the typical sub-bituminous coal in China, and the coal molecular model of which was constructed based on proximate analysis, ultimate analysis, C-NMR, and XPS. The chemical structure model of the coal molecule was optimized, and the periodic boundary condition was added via the method of molecular dynamics methods. The different combined systems formed by collectors, water, and a model surface of Bulianta coal have been studied using molecular dynamics simulation. The simulation results of dodecanol and methyl laurate on the surface of Bulianta coal show that dodecanol molecules are not evenly adsorbed on the surface of coal, and have higher adsorption capacity near carboxyl and hydroxyl groups, but less adsorption capacity near carbonyl and ether bonds. Methyl laurate can completely cover the oxygen-containing functional groups on the coal surface. Compared with dodecanol, methyl laurate can effectively improve the hydrophobicity of the Bulianta coal surface, which is consistent with the results of the XPS test and the flotation test.
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http://dx.doi.org/10.3390/molecules25174030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504768PMC
September 2020
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