Publications by authors named "Wenli Feng"

91 Publications

Experimental study on abnormal thyroid function in patients with Hashimoto's Thyroiditis caused by interference of thyroid hormone autoantibodies.

Endokrynol Pol 2021 Dec 2. Epub 2021 Dec 2.

Department of Clinical laboratory, Tianjin Medical University General Hospital, No.154 Anshan Road, Heping District, 300052 Tianjin, China.

Introduction: Thyroid hormone autoantibody (THAAb) is one of the important factors affecting the measurement of thyroid function. By studying the results of a patient suffered with Hashimoto's thyroiditis, we fully communicated with the clinician, looked for reasons, and achieved the purpose of restoring the truth and sorting out solutions.

Material And Methods: During routine examination by ADVIA-Centaur XP system, we found the test of a case was inconsistent with her clinical manifestations, with abnormal elevation of free thyroxine (FT4) that did not conform to the rule of the hypothalamic-pituitary-thyroid axis. Then, different platforms and demonstration of THAAbs with polyethylene glycol (PEG) precipitation were performed to eliminate the influence of THAAbs.

Results: The results showed that the thyroid function of the patient was consistent with the clinical manifestations and conformed to the law of the hypothalamic-pituitary-thyroid axis at Architect-i2000sr platform and Roche-Cobas-601 system. The content of FT4 was significantly reduced and lower than the normal reference range, after the patients' serum was treated with PEG, which was in line with the clinical practice. The serum THAAb titer of the patients was nearly 100 times higher than that of the control group.

Conclusions: Once the thyroid function of the patients do not conform to their own laws and clinical manifestations, laboratory staff should consider the interference of THAAbs. It is necessary to change the detection platform and retest the serum after PEG treatment while communicating with the clinicians, which is of great significance to provide a true and accurate result to clinicians and patients.
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http://dx.doi.org/10.5603/EP.a2021.0090DOI Listing
December 2021

Silica-supported near-infrared carbon dots and bicarbonate nanoplatform for triple synergistic sterilization and wound healing promotion therapy.

J Colloid Interface Sci 2021 Oct 28;608(Pt 2):1308-1322. Epub 2021 Oct 28.

Jiangsu Collaborative Innovation Center for Biological Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China; Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Engineering Research Center for Biomedical Function Materials, Nanjing 210023, China. Electronic address:

Widespread bacterial infection and the emergence of antibiotic resistance exhibit an increasing threat to public health. Additionally, chronic wounds caused by bacterial infection have become a major challenge and threat in medical. Therefore, it is of great significance to explore effective and safe nanomaterials which possess antibacterial and wound healing promotion performance. Herein, we developed silica-supported near-infrared carbon dots ([email protected]) and bicarbonate (BC) nanoplatform (BC/[email protected]@PDA), which possess triple synergistic antibacterial including quaternary ammonium compounds (QACs), photothermal therapy (PTT), and photodynamic therapy (PDT). Meanwhile, the nanoplatform realized the controlled release of CO in situ triggered by 808 nm laser irradiation for wound healing. In vitro and in vivo antibacterial assays displayed that the BC/[email protected]@PDA possess excellent antibacterial property, the antibacterial rate up to 99.6% and 99.99% to Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), respectively. Wound healing evaluation proved that suitable release of CO could promote the process of infected wound healing, and the wound healing rate up to 100% after treatment for 14 days. Additionally, the cellular imaging experiment revealed that the BC/[email protected]@PDA could be considered as fluorescence probe. Together, these results demonstrated that the BC/[email protected]@PDA have great potential in biomedical field.
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http://dx.doi.org/10.1016/j.jcis.2021.10.147DOI Listing
October 2021

LncRNA OIP5-AS1 Promotes the Autophagy-Related Imatinib Resistance in Chronic Myeloid Leukemia Cells by Regulating miR-30e-5p/ATG12 Axis.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211052150

12550Chongqing Medical University, Chongqing, China.

Resistance to tyrosine kinase inhibitors (TKIs) in patients with chronic myeloid leukemia (CML) remains a problem in clinical treatment, and the mechanism has not been fully clarified. Autophagy can protect cancer cells under chemotherapeutic stimulation. Long noncoding RNAs (lncRNAs) are critical in drug resistance of CML. The role of lncRNAs in autophagy and drug resistance of CML needs to be further explored. Western blot and immunofluorescence were used to evaluate the autophagy activity in the drug-resistant CML cell line K562/G01 and its parental cell line K562. Then the sensitivity of K562/G01 cells to the first generation TKI imatinib (IM) after autophagy inhibition was determined by CCK-8 assays. The lncRNA OIP5-AS1 related to the drug resistance of CML cells was determined by Gene Expression Omnibus database analysis. Western blot and drug-sensitivity assays were used to detect changes in autophagy and sensitivity to the IM in resistant CML cells after OIP5-AS1 knockdown. The interactions of OIP5-AS1, miR-30e-5p, and ATG12 were explored by RNA immunoprecipitation and dual-luciferase reporter assays. In this study, we found that autophagy was associated with drug resistance in CML cells. Moreover, the upregulation of OIP5-AS1 in K562/G01 cells was related to the enhancement of autophagy. Knockdown of OIP5-AS1 suppressed autophagy and enhanced the sensitivity of K562/G01 cells to IM. Furthermore, OIP5-AS1 regulated ATG12 by competitively binding miR-30e-5p, thereby affecting autophagy-related drug resistance. Our study reveals that OIP5-AS1 promotes the autophagy-related IM resistance in CML cells by regulating miR-30e-5p/ATG12 axis, providing new insights into the drug resistance mechanism of CML.
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http://dx.doi.org/10.1177/15330338211052150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564130PMC
November 2021

Self-Assembled Nanosized Vehicles from Amino Acid-Based Amphiphilic Polymers with Pendent Carboxyl Groups for Efficient Drug Delivery.

Biomacromolecules 2021 11 12;22(11):4871-4882. Epub 2021 Oct 12.

State Key Laboratory of Organic-Inorganic Composites, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.

Developing safe and efficient delivery vehicles for chemotherapeutic drugs has been a long-standing demanding. Amino acid-based polymers are promising candidates to address this challenge due to their excellent biocompatibility and biodegradation. Herein, a series of well-defined amphiphilic block copolymers were prepared by PET-RAFT polymerization of -acryloyl amino acid monomers. By altering monomer types and the block ratio of the copolymers, the copolymers self-assembled into nanostructures with various morphologies, including spheres, rod-like, fibers, and lamellae via hydrophobic and hydrogen bonding interactions. Significantly, the nanoparticles (NPs) assembled from amphiphilic block copolymers poly(-acryloyl-valine)--poly(-acryloyl-aspartic acid) (PV--PD) displayed an appealing cargo loading efficiency (21.8-32.6%) for a broad range of drugs (paclitaxel, doxorubicin (DOX), cisplatin, etc.) due to strong interactions. The DOX-loaded PV--PD NPs exhibited rapid cellular uptake (within 1 min) and a great therapeutic performance. These drug delivery systems provide new insights for regulating the controlled morphologies and improving the efficiency of drug delivery.
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http://dx.doi.org/10.1021/acs.biomac.1c01164DOI Listing
November 2021

Pancreatic neuroendocrine tumor producing vasopressin: A case report.

Medicine (Baltimore) 2021 Oct;100(40):e27453

Department of Endocrinology and Metabolism, Tianjin Medical University General Hospital, Tianjin, China.

Rationale: Functional pancreatic neuroendocrine tumors (pNETs) rarely produce vasopressin. Here, we reported a case of pNET producing vasopressin in a 78-year-old man with hyponatremia.

Patient Concerns: The patient presented with anorexia approximately 4 years ago, and the laboratory test results indicated hyponatremia. He was hospitalized 3 times subsequently due to anorexia in the past 4 years, during which laboratory tests consistently indicated severe hyponatremia.

Diagnosis: Upon admission, his serum osmolarity, urine osmolarity, urine sodium level, and 24-hour urine sodium level was 277 mOsm/kg H2O, 465 mOsm/kg H2O, 82.5 mmol/L, and 140.25 mmol, respectively. Gallium-68-labeled tetraazacyclododecanetetraacetic acid-Dphel-Tyr3-octreotate positron emission tomography-computed tomography showed a high uptake lesion measuring approximately 1 cm in diameter in the pancreatic body, and the possibility of pNET was considered. Besides, laboratory tests showed that adrenocorticotropic hormone, follicle-stimulating hormone, and luteinizing hormone released by the pituitary was insufficient in the case of low levels of cortisol, estradiol, progesterone, and testosterone. Thus, the diagnosis of the syndrome of inappropriate antidiuresis (SIAD) was considered along with hypopituitarism.

Interventions: The patient underwent surgery, and pNET was confirmed by pathology examination. The immunohistochemical study showed that the tumor cells were positive for somatostatin receptors 2 and vasopressin.

Outcomes: In the last follow-up 17 months after surgery, the patient was in good condition, taking methylprednisolone 4 mg every other day, and had been free of anorexia or hyponatremia episodes.

Lessons: This case illustrated the potential ectopic production of vasopressin resulting in SIAD in pNETs, highlighting the adoption of gallium-68-labeled tetraazacyclododecanetetraacetic acid-Dphel-Tyr3-octreotate positron emission tomography-computed tomography and vasopressin immunohistochemical staining in the evaluation of the etiology of SIAD.
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http://dx.doi.org/10.1097/MD.0000000000027453DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500607PMC
October 2021

RanBP3 Regulates Proliferation, Apoptosis and Chemosensitivity of Chronic Myeloid Leukemia Cells Mediating SMAD2/3 and ERK1/2 Nuclear Transport.

Front Oncol 2021 24;11:698410. Epub 2021 Aug 24.

Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, Department of Clinical Hematology, Chongqing Medical University, Chongqing, China.

Abnormal subcellular localization of proteins is an important cause of tumorigenesis and drug resistance. Chromosome region maintenance 1 (CRM1), the nuclear export regulator of most proteins, has been confirmed to be over-expressed in various malignancies and is regarded as an efficient target. But the potential role of the CRM1 cofactor RanBP3 (Ran Binding Protein 3) is left unrevealed in chronic myeloid leukemia (CML). Here, we first detected the level of RanBP3 in CML and found an elevated RanBP3 expression in CML compared with control. Then we used shRNA lentivirus to down-regulated RanBP3 in imatinib sensitive K562 cells and resistant K562/G01 cells and found RanBP3 silencing inhibited cell proliferation by up-regulating p21, induced caspase3-related cell apoptosis, and enhanced the drug sensitivity of IM . Notably, we observed that RanBP3 silencing restored imatinib sensitivity of K562 cells in NOD/SCID mice. Mechanistically, the nuclear aggregation of SMAD2/3 revealed that tumor suppressor axis (TGF-β)-SMAD2/3-p21 was the anti-proliferation program related to RanBP3 knockdown, and the decrease of cytoplasmic ERK1/2 caused by RanBP3 interference leaded to the down-regulation of anti-apoptosis protein p(Ser112)-BAD, which was the mechanism of increased cell apoptosis and enhanced chemosensitivity to imatinib in CML. In summary, this study revealed the expression and potential role of RanBP3 in CML, suggesting that targeting RanBP3 alone or combined with TKIs could improve the clinical response of CML.
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http://dx.doi.org/10.3389/fonc.2021.698410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421687PMC
August 2021

Intracellular delivery of anti-BCR/ABL antibody by PLGA nanoparticles suppresses the oncogenesis of chronic myeloid leukemia cells.

J Hematol Oncol 2021 09 6;14(1):139. Epub 2021 Sep 6.

Department of Clinical Hematology, School of Laboratory Medicine, Chongqing Medical University, No. 1, Yixueyuan Road, Yuzhong District, Chongqing, 400016, China.

Background: The pathogenesis of chronic myeloid leukemia (CML) is the formation of the BCR/ABL protein, which is encoded by the bcr/abl fusion gene, possessing abnormal tyrosine kinase activity. Despite the wide application of tyrosine kinase inhibitors (TKIs) in CML treatment, TKIs drug resistance or intolerance limits their further usage in a subset of patients. Furthermore, TKIs inhibit the tyrosine kinase activity of the BCR/ABL oncoprotein while failing to eliminate the pathologenic oncoprotein. To develop alternative strategies for CML treatment using therapeutic antibodies, and to address the issue that antibodies cannot pass through cell membranes, we have established a novel intracellular delivery of anti-BCR/ABL antibodies, which serves as a prerequisite for CML therapy.

Methods: Anti-BCR/ABL antibodies were encapsulated in poly(D, L-lactide-co-glycolide) nanoparticles (PLGA NPs) by a double emulsion method, and transferrin was labeled on the surface of the nanoparticles ([email protected] NPs). The characteristics of nanoparticles were measured by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Cellular uptake of nanoparticles was measured by flow cytometry (FCM). The effect of nanoparticles on the apoptosis and proliferation of CML cells was testified by FCM and CCK-8 assay. In addition, the anti-cancer impact of nanoparticles was evaluated in mouse models of CML.

Results: The results demonstrated that the [email protected] NPs functioned as an intracellular deliverer of antibodies, and exhibited an excellent effect on degrading BCR/ABL oncoprotein in CML cells via the Trim-Away pathway. Treatment with [email protected] NPs inhibited the proliferation and induced the apoptosis of CML cells in vitro as well as impaired the oncogenesis ability of CML cells in vivo.

Conclusions: In conclusion, our study indicated that this approach achieved safe and efficient intracellular delivery of antibodies and degraded BCR/ABL oncoprotein via the Trim-Away pathway, which provides a promising therapeutic strategy for CML patients, particularly those with TKI resistance.
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http://dx.doi.org/10.1186/s13045-021-01150-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422775PMC
September 2021

A novel fusion circular RNA F-circBA1 derived from the fusion gene displayed an oncogenic role in chronic myeloid leukemia cells.

Bioengineered 2021 12;12(1):4816-4827

Department of Clinical Hematology, School of Laboratory Medicine, Chongqing Medical University, Chongqing, China.

The fusion gene plays a crucial role in the leukemogenesis of chronic myeloid leukemia (CML). The BCR-ABL oncoprotein encoded by this fusion gene has been extensively studied. However, research on whether also affects circular RNAs (circRNAs) is limited. This study aimed to explore the new fusion circRNAs produced by and their role in CML cells. In this study, we identified a novel fusion circRNA, named F-circBA1, originating from in K562 and K562/G01 cells using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and Sanger sequencing. qRT-PCR of the nuclear RNA and cytoplasmic RNA were separated, indicating that F-circBA1 was mainly localized in the cytoplasm. Cell counting kit-8 assay and flow cytometry showed that F-circBA1 knockdown by shRNA prevented the proliferation of K562 and K562/G01 cells, and the cell cycle was arrested at G2/M. Mechanically, dual-luciferase reporter assay and western blotting assay showed that F-circBA1 sponged miR-148-3p and F-circBA1 silencing decreased CDC25B expression in vitro. Furthermore, the results of the murine leukemogenesis model showed that F-circBA1 knockdown suppressed leukemogenesis in vivo. Besides, we found the existence of F-circBA1 in some patients with -positive CML. In conclusion, these results demonstrate the presence of F-circBA1 and its oncogenic role in CML cells.
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http://dx.doi.org/10.1080/21655979.2021.1957749DOI Listing
December 2021

Effects of combined soil amendments on Cd accumulation, translocation and food safety in rice: a field study in southern China.

Environ Geochem Health 2021 Jul 19. Epub 2021 Jul 19.

Institute of Environmental Engineering, School of Metallurgy and Environment, Central South University, Changsha, 410083, China.

Excessive Cd content and high Cd/Zn ratio in rice grains threaten human health. To study the reduction effects of combined soil amendments on Cd content and Cd/Zn ratio in rice planting in soils with different Cd contamination levels, we conducted field trials in three regions of Hunan province, China. Six field treatments were designed in each study area, including control (CK), lime alone (L), lime combined with sepiolite (LS), phosphate fertilizer (LP), organic fertilizer (LO) and phosphate fertilizer + organic fertilizer (LPO). The application of the combined amendments reduced the Cd content in rice grains to less than the Food Health Standard of China (0.2 mg/kg) and the Cd/Zn ratio to less than the safety threshold of 0.015. The average reduction rates of grain Cd content under the combined treatments among the three regions increased with the increase in Cd content in the soil. Meanwhile, the amendments also decreased the soil available Cd and Zn concentration significantly. The LO had the highest efficiency on decreasing Cd content in rice grains among these amendments, which is ranged from 44.6% to 52.8% in the three regions compared with CK. Similarly, high reduction rates of Cd/Zn ratio were found in the LO treatment, with an average value of 57.3% among the three regions. The grain Cd contents and Cd/Zn ratios were significantly correlated with the soil available Cd concentrations, plant uptake factor and the straw to rice grain translocation factor (TF) (P < 0.05). The results indicated that the combined soil amendments, especially lime combined with organic fertilizer, would be an effective way to control Cd content in rice.
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http://dx.doi.org/10.1007/s10653-021-01033-7DOI Listing
July 2021

Effect of HSP90AB1 and CC domain interaction on Bcr-Abl protein cytoplasm localization and function in chronic myeloid leukemia cells.

Cell Commun Signal 2021 07 3;19(1):71. Epub 2021 Jul 3.

Department of Clinical Hematology, Key Laboratory of Laboratory Medical Diagnostics Designated By Ministry of Education, School of Laboratory Medicine, Chongqing Medical University, No.1, Yixueyuan Road, Yuzhong District, Chongqing, 400016, China.

Background: The fusion oncoprotein Bcr-Abl is mostly located in the cytoplasm, which causes chronic myeloid leukemia (CML). After moving into the nucleus, the fusion protein can induce apoptosis of CML cells. The coiled-coil domain (CC domain) of Bcr-Abl protein plays a central role in the subcellular localization. However, how CC domain affects subcellular localization of Bcr-Abl remains unclear.

Methods: Herein, the key proteins interacting with the Bcr-Abl CC domain were screened by immunoprecipitation binding mass spectrometry. The specific site of Bcr-Abl CC domain binding to target protein was predicted by Deep Viewer. Immunoprecipitation assay was used to confirmed the specific sites of protein binding. IF and western blot were used to observe the subcellular localization of target protein. Western blot was used to examine the protein changes. CCK-8, clonal formation test and FCM cycle detection were used to observe the effect of inhibitor on the proliferation ability of CML cells. FCM apoptosis detection was used to observe the level of cells apoptosis.

Results: HSP90AB1 interacts with Bcr-Abl CC domain via N-terminal domain (NTD), preventing the transport of Bcr-Abl protein to the nucleus and maintaining the activation of Bcr-Abl tyrosine kinase. The nucleus-entrapped Bcr-Abl markedly inhibits the proliferation and induces apoptosis of CML cells by activating p73 and repressing the expression of cytoplasmic oncogenic signaling pathways mediated by Bcr-Abl. Moreover, the combination of 17AAG (Tanespimycin) with Leptomycin B (LMB) considerably decreased the proliferation of CML cells.

Conclusion: Our study provides evidence that it is feasible to transport Bcr-Abl into the nucleus as an alternative strategy for the treatment of CML, and targeting the NTD of HSP90AB1 to inhibit the interaction with Bcr-Abl is more accurate for the development and application of HSP90 inhibitor in the treatment of CML and other Bcr-Abl-addicted malignancies. Video abstract.
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http://dx.doi.org/10.1186/s12964-021-00752-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254927PMC
July 2021

Targeting FEN1 Suppresses the Proliferation of Chronic Myeloid Leukemia Cells Through Regulating Alternative End-Joining Pathways.

DNA Cell Biol 2021 Aug 22;40(8):1101-1111. Epub 2021 Jun 22.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Chronic myeloid leukemia (CML) is characterized by the formation of the fusion gene. The BCR-ABL protein leads to an increased level of reactive oxygen species, which is a major cause of endogenous DNA double-strand breaks (DSBs). CML cells are prone to rely on a highly mutagenic alternative end-joining (Alt-EJ) pathway to cope with enhanced DSBs, which aggravates chromosomal instability. Hence, targeting dysregulated DNA repair proteins provides new insights into cancer treatment. In this study, we discovered the abnormal upregulation of Flap endonuclease 1 (FEN1) in CML, as well as FEN1's participation in the error-prone Alt-EJ repair pathway and its interplay with DNA Ligase1 and proliferating cell nuclear antigen in DSB repair. Knockdown of FEN1 by shRNA not only inhibited the proliferation and induced apoptosis but also enhanced the efficacy of imatinib (IM) in drug-resistant CML cell K562/G01. Moreover, excessive DSB accumulation was detected after FEN1 inhibition. In summary, our results demonstrated that FEN1 is a promising therapeutic target in CML treatment. This work extends the understanding of regulating abnormal DSB repair for cancer treatment.
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http://dx.doi.org/10.1089/dna.2021.0239DOI Listing
August 2021

Cotreatment with Aspirin and Azole Drugs Increases Sensitivity of in vitro.

Infect Drug Resist 2021 2;14:2027-2038. Epub 2021 Jun 2.

The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Purpose: This study aimed to investigate the effects of aspirin (acetyl salicylic acid [ASA]) combined with fluconazole (FCA), itraconazole (ITR), or voriconazole (VRC) on under planktonic and biofilm conditions.

Methods: A total of 39 clinical strains were used to perform the in vitro drug sensitivity assay under different conditions using the M27-A4 broth microdilution method. The minimal inhibitory concentrations (MICs) and fractional inhibitory concentration index (FICI) values were calculated. ZY23 was chosen for the further analyses.

Results: Under planktonic conditions, the half maximal MIC (MIC) values of FCA, ITR, and VRC were 64-0.5 μg/mL, 32-0.0625 μg/mL, and 16-0.125 μg/mL, respectively, when applied, whereas in combination with ASA, the values decreased to 32-0.25 μg/mL, 8-0.0313 μg/mL, and 8-0.0313 μg/mL, respectively. Under biofilm conditions, FCA, ITR, or VRC alone showed MIC values of 128-8 μg/mL, 32-4 μg/mL, and 32-0.5 μg/mL, whereas in combination with ASA the values were decreased to 32-0.5 μg/mL, 16-0.5 μg/mL, and 8-0.0625 μg/mL, respectively. Analysis of the FICI showed that the sensitization rate of ASA to FCA, ITR, and FCA under planktonic conditions was 43.59%, whereas the sensitization rates of ASP to FCA, ITR, and FCA under biofilm conditions were 46.15%, 46.15%, and 48.72%, respectively. Additionally, the time-growth and time-kill curves of ZY23 further verified the synergistic effects of ASA on azole drugs.

Conclusion: ASA may act as an enhancer of the inhibitory effects of azole drugs on the growth of clinical under planktonic and biofilm conditions.
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http://dx.doi.org/10.2147/IDR.S314538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180266PMC
June 2021

Functional characterization of a loss-of-function mutant I324M of arginine vasopressin receptor 2 in X-linked nephrogenic diabetes insipidus.

Sci Rep 2021 05 26;11(1):11057. Epub 2021 May 26.

Department of Endocrinology and Metabolism, Tianjin Medical University General Hospital, Tianjin, 300052, China.

X-linked nephrogenic diabetes insipidus (X-linked NDI) is a rare inherited disease mainly caused by lost-of-function mutations in human AVPR2 gene encoding arginine vasopressin receptor 2 (V2R). Our focus of the current study is on exploration of the functional and biochemical properties of Ile324Met (I324M) mutation identified in a pedigree showing as typical recessive X-linked NDI. We demonstrated that I324M mutation interfered with the conformation of complex glycosylation of V2R. Moreover, almost all of the I324M-V2R failed to express on the cell surface due to being captured by the endoplasmic reticulum control system. We further examined the signaling activity of DDAVP-medicated cAMP and ERK1/2 pathways and the results revealed that the mutant receptor lost the ability in response to DDAVP stimulation contributed to the failure of accumulation of cAMP and phosphorylated ERK1/2. Based on the characteristics of molecular defects of I324M mutant, we selected two reagents (SR49059 and alvespimycin) to determine whether the functions of I324M-V2R can be restored and we found that both compounds can significantly "rescue" I324M mutation. Our findings may provide further insights for understanding the pathogenic mechanism of AVPR2 gene mutations and may offer some implications on development of promising treatments for patients with X-linked NDI.
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http://dx.doi.org/10.1038/s41598-021-90736-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154955PMC
May 2021

The effects of secreted aspartyl proteinase inhibitor ritonavir on azoles-resistant strains of Candida albicans as well as regulatory role of SAP2 and ERG11.

Immun Inflamm Dis 2021 09 5;9(3):667-680. Epub 2021 May 5.

The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.

Background: Candida albicans, the main human fungal pathogen, can cause fungal infection and seriously affect people's health and life. This study aimed to investigate the effects of ritonavir (RIT) on C. albicans and the correlation between SAP2 as well as ERG11 and drug resistance.

Results: Secreted aspartyl proteinases (Saps) activities and pathogenicity of C. albicans with different drug resistance were measured. M27-A4 broth microdilution method was used to analyze the drug sensitivity of RIT combined with fluconazole (FCA) on C. albicans. After that, SAP2 and ERG11 mutations were examined by polymerase chain reaction (PCR) and sequencing, and quantitative real-time PCR was utilized to determine the expression of the two genes. By analyzing pz values, the Saps activity of cross-resistant strains was the highest, followed by voriconazole (VRC)-resistant strains, FCA-resistant strains, itraconazole (ITR)-resistant strains, and sensitive strains. The pathogenicity of C. albicans in descending order was as follows: cross-resistant strains, VRC-resistant strains, ITR-resistant strains, FCA-resistant strains, and sensitive strains. With the increase of RIT concentrations, the Saps activity was gradually inhibited. Drug sensitivity results showed that there was no synergistic effect between RIT and FCA. Additionally, no gene mutation sites were found in SAP2 sequencing, and 17 synonymous mutations and 6 missense mutations occurred in ERG11 sequencing. Finally, the expression of SAP2 and ERG11 was significantly higher in the resistant strains compared with the sensitive strains, and there was a positive liner correlation between SAP2 and ERG11 messenger RNA expression (r = .6655, p < .001).

Conclusion: These findings may help to improve our understanding of azole-resistant mechanisms of C. albicans and provide a novel direction for clinical therapeutics of C. albicans infection.
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http://dx.doi.org/10.1002/iid3.415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342201PMC
September 2021

Poly(amino acid)s-based star AIEgens for cell uptake with pH-response and chiral difference.

Colloids Surf B Biointerfaces 2021 Jun 9;202:111687. Epub 2021 Mar 9.

Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address:

Chiral aggregation-induced emission luminogens (AIEgens) are the new-generation chiral sensors that regulate chiral signals from the molecular level to the macroscopic assembly. Expanding applications of chiral AIEgens and in-depth understanding of their chiral recognition in biological systems are meaningful. Herein, two star chiral AIEgens, consisting of tetraphenylethene (TPE) as core and poly(N-acryloyl-L(D) valine) (PLV or PDV) as arms, were precisely synthesized via atom transfer radical polymerization (ATRP) technique and named TPE-PLV and TPE-PDV. They possessed typical AIE characteristics and exhibited an increase in concentration-dependent fluorescence intensity. The two AIEgens were pH-responsive and had strong AIE-related emission in acidic solution. Importantly, AIEgens can enter the living cells by ATP dependent endocytosis, then light them up. The interactions between the AIEgens and living human hepatocarcinoma (HepG2) cells revealed that the internalization process of TPE-PLV and TPE-PDV was both chiral-dependent and pH-responsive. This novel strategy for synthesizing poly(amino acid)s functionalized AIEgens could inspire the development of promising fluorescent materials with chirality.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111687DOI Listing
June 2021

Peritoneal resident macrophages in mice with MLL-AF9-induced acute myeloid leukemia show an M2-like phenotype.

Ann Transl Med 2021 Feb;9(3):266

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Background: Acute myeloid leukemia (AML) is a devastating disease with a poor prognosis. Innate and adaptive immunity is closely related to the progression of leukemia. Macrophages within the leukemic microenvironment have a tendency toward a leukemia-permissive phenotype. However, the characteristics of macrophages in leukemia, including their kinetics, gene expression, and functional roles have not been fully illuminated.

Methods: In the current study, the characteristics of peritoneal resident macrophages, which were large peritoneal macrophages (LPM), from mice with mixed lineage leukemia (MLL)-AF9-induced AML were investigated. AML-associated large macrophages (AML-LPM) were gated as F4/80 MHC-II by flow cytometry. To further investigate the relationship between the leukemic microenvironment and macrophage characteristics, RNA sequencing was performed. Meanwhile, apoptosis, killing ability, and phagocytic function of peritoneal resident macrophages in MLL-AF9-induced AML were assessed.

Results: The results suggested that AML microenvironment was found to affect the kinetics and morphology of peritoneal resident macrophages. The results of RNA sequencing suggested that the gene expression of AML-LPMs differed significantly from that of normal LPMs. The AML microenvironment also had effects on the apoptosis, killing ability, and phagocytic function of peritoneal resident macrophages.

Conclusions: These data suggest that peritoneal resident macrophages in mice with AML induced by MLL-AF9 show an M2-like phenotype. The reversal of macrophage polarization in the leukemic microenvironment may potentially enhance the immunotherapeutic effect in AML.
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http://dx.doi.org/10.21037/atm-21-139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940882PMC
February 2021

Virulence of "white-gray-opaque" tri-stable transformation in clinical Candida albicans in vitro and in vivo.

Microb Pathog 2021 May 6;154:104825. Epub 2021 Mar 6.

The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, China. Electronic address:

The study aimed to induce the white-opaque-gray tri-stable transformation in clinical C. albicans and to explore their potential pathogenicity. Sixty-four clinical strains were used to induce the white, opaque and gray cells of C. albicans. Secreted aspartyl proteinases (Sap) activity of the three phenotypes was then measured, and a vulvovaginal candidiasis (VVC) animal model was constructed. Of the 64 clinical strains, only 3 strains successfully underwent white-gray-opaque tri-stable transformation, and the three strains all belonged to MTL homozygous strains. Pz values in white, opaque and gray phenotypes were 0.834 ± 0.012, 0.707 ± 0.036, and 0.628 ± 0.002, respectively, which indicated that the cells with gray phenotype had higher Sap activity. After inoculation of different fungal suspension, the fungal colony count in descending order was as follows: gray phenotype, opaque phenotype and white phenotype. After treated with fluconazole for 3 days or 10 days, the fungal colony counts were significantly decreased compared with that before treatment (P < 0.05). The Sap activity and pathogenicity of gray cells in C. albicans were the strongest, followed by opaque cells and white cells. Additionally, white, gray and opaque phenotypic cells were all susceptible to fluconazole.
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http://dx.doi.org/10.1016/j.micpath.2021.104825DOI Listing
May 2021

TCF7 knockdown inhibits the imatinib resistance of chronic myeloid leukemia K562/G01 cells by neutralizing the Wnt/β‑catenin/TCF7/ABC transporter signaling axis.

Oncol Rep 2021 02 27;45(2):557-568. Epub 2020 Nov 27.

Department of Clinical Hematology, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.

Clinical resistance to ABL tyrosine kinase inhibitor (TKI) imatinib remains a critical issue in the treatment of chronic myeloid leukemia (CML). Transcription factor 7 (TCF7) is one of the main Wnt/β‑catenin signaling mediators. Previous studies have shown that TCF7 is vital for tumor initiation, and targeting TCF7 can reduce drug resistance in many types of cancer. However, the role of TCF7 in CML imatinib‑resistant cells is unclear. In the present study, we analyzed the transcriptomic data from CML clinical samples in the Gene Expression Omnibus (GEO) and performed experimental verification in the CML imatinib‑resistant cell line K562/G01. We found that the expression of TCF7 was independent of BCR‑ABL1 activity. Silencing of TCF7 downregulated the expression levels of CTNNB1, CCND1, and ABCC2, and therefore inhibited proliferation, weakened colony formation, and increased the drug sensitivity of imatinib‑resistant cells. After analyzing the transcriptomic data of four groups (Scramble, TCF7_KD, Scramble+imatinib, and TCF7_KD+imatinib) using bioinformatics, we noted that Wnt/β‑catenin and ATP‑binding cassette (ABC) transporter signaling pathways were upregulated in imatinib‑resistant cells under conventional dose of imatinib, and TCF7 knockdown could neutralize this effect. Next, using ChIP‑qPCR, we demonstrated that TCF7 was recruited to the promoter region of ABCC2 and activated gene transcription. In summary, our results highlight that the upregulation of Wnt/β‑catenin and ABC transporter signaling pathways induced by imatinib treatment of resistant cells confers imatinib resistance, and reveal that targeting TCF7 to regulate the Wnt/β‑catenin/TCF7/ABC transporter signaling axis may represent an effective strategy for overcoming imatinib resistance.
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http://dx.doi.org/10.3892/or.2020.7869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757089PMC
February 2021

A dual recognition strategy for accurate detection of CTCs based on novel branched PtAuRh trimetallic nanospheres.

Biosens Bioelectron 2021 Mar 15;176:112893. Epub 2020 Dec 15.

Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address:

Accurate detection of circulating tumor cells (CTCs) has a pivotal role in the metastasis monitoring and prognosis of tumor. In this work, an ultrasensitive electrochemical cytosensor was developed based on excellent electrocatalytic materials and a dual recognition strategy. Herein, novel branched PtAuRh trimetallic nanospheres (b-PtAuRh TNS) were synthesized for the first time by a facile one-pot method, which had a huge specific surface area and outstanding catalytic activity. B-PtAuRh TNS linked with aptamers targeting mucin1 (MUC1) were served as signal tags to amplify the signal. As electrode modified material, the nanocomposites of Cabot carbon black (BP2000) and AuNPs were used to improve the electron transfer efficiency of electrode. In addition to using b-PtAuRh TNS labeled anti-MUC1 aptamers as signal probes, anti-EpCAM antibodies were worked as capture probes to achieve dual recognition of target cells. In other words, only cells expressing both MUC1 and EpCAM could produce electrochemical signal. The constructed cytosensor presented a wide linear range (5 - 1 × 10 cells mL) and a low detection limit (1 cell mL). It was worth noting that the proposed cytosensor could detect CTCs in clinical blood samples. To sum up, the developed cytosensor might become a promising detection platform for cancer diagnosis and tumor metastasis.
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http://dx.doi.org/10.1016/j.bios.2020.112893DOI Listing
March 2021

Catalytic hydrothermal liquefaction of Gracilaria corticata macroalgae: Effects of process parameter on bio-oil up-gradation.

Bioresour Technol 2021 Jan 25;319:124163. Epub 2020 Sep 25.

Department of Chemical and Biomedical Engineering, Center for Innovation in Gas Research and Utilization, West Virginia University, Morgantown, WV 26506, USA.

Hydrothermal liquefaction (HTL) of Gracilaria corticata (GC) macroalgae was studied over a series of nickel-iron-layered double oxides (NiFe-LDO) supported on activated bio-char catalysts at 280 °C and different solvents medium. Maximum bio-oil yield (56.2 wt%) was found with 5%Ga/NiFe-LDO/AC catalyst at 280 °C under ethanol solvent. The catalytic HTL up-gradation decreased the bio-char yield significantly. However the bio-oil quality significantly improved with using the 5%Ga/NiFe-LDO/AC catalyst. Also, improved performance with higher amount of bio-oil and lower amounts of bio-char and gas were achieved, which is due the several reactions happening during the HTL process. Catalytic HTL also revealed that introducing NiFe-LDO nanosheets into the activated char could result in NiFe-LDO/AC catalysts of higher surface area and increased active sites. Being impregnated by 5%Ga, catalysts with improved acid sites and thereby, advanced deoxygenation and aromatization activities were achieved. Hence Ga/NiFe-LDO/AC could be considered as a promising catalyst HTL bio-oil upgrading.
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http://dx.doi.org/10.1016/j.biortech.2020.124163DOI Listing
January 2021

Aspirin and verapamil increase the sensitivity of Candida albicans to caspofungin under planktonic and biofilm conditions.

J Glob Antimicrob Resist 2021 03 23;24:32-39. Epub 2020 Nov 23.

Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, No. 382 Wuyi Road, Taiyuan 030001, Shanxi, China.

Objectives: This study aimed to investigate the effects of caspofungin (CAS) combined with aspirin (ASP) or verapamil (VPL) on the sensitivity of Candida albicans under planktonic and biofilm conditions.

Methods: A total of 39 C. albicans clinical strains were used to construct biofilms. Sensitivity to ASP or VPL combined with CAS was analysed by broth microdilution. MIC values were obtained and the fractional inhibitory concentration index (FICI) was calculated. Subsequently, C. albicans ZY22 was selected for time-growth curve analysis and strains ZY15 and ZY22 were used for time-kill curve analysis.

Results: Under planktonic condition the MIC of CAS was 0.0313-8 μg/mL following treatment with CAS alone, whereas it decreased to 0.0313-4 μg/mL following CAS combined with ASP or VPL. Under biofilm condition the MIC of CAS was 0.125-16 μg/mL following treatment with CAS alone, whereas it decreased to 0.0625-16 μg/mL or 0.0625-8 μg/mL following CAS combined with ASP or VPL. FICI results showed synergistic interactions between CAS and ASP under planktonic and biofilm conditions in 17 and 16 strains, respectively. However, synergistic interactions between CAS and VPL under planktonic and biofilm conditions were observed in 19 and 23 strains, respectively. Additionally, 8000 μg/mL ASP or 8 μg/mL VPL combined with CAS had better inhibitory effects on C. albicans.

Conclusion: ASP and VPL may be a sensitiser for CAS, and the antifungal effects of CAS may be sensitised by 8000 μg/mL ASP or 8 μg/mL VPL against C. albicans under planktonic and biofilm conditions.
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http://dx.doi.org/10.1016/j.jgar.2020.11.013DOI Listing
March 2021

Biological behaviors of mutant proinsulin contribute to the phenotypic spectrum of diabetes associated with insulin gene mutations.

Mol Cell Endocrinol 2020 12 8;518:111025. Epub 2020 Sep 8.

Department of Endocrinology and Metabolism, Tianjin Medical University General Hospital, Tianjin, China. Electronic address:

Insulin gene mutation is the second most common cause of neonatal diabetes (NDM). It is also one of the genes involved in maturity-onset diabetes of the young (MODY). We aim to investigate molecular behaviors of different INS gene variants that may correlate with the clinical spectrum of diabetes phenotypes. In this study, we concentrated on two previously uncharacterized MODY-causing mutants, proinsulin-p.Gly44Arg [G(B20)R] and p.Pro52Leu [P(B28)L] (a novel mutant identified in one French family), and an NDM causing proinsulin-p.(Cys96Tyr) [C(A7)Y]. We find that these proinsulin mutants exhibit impaired oxidative folding in the endoplasmic reticulum (ER) with blocked ER export, ER stress, and apoptosis. Importantly, the proinsulin mutants formed abnormal intermolecular disulfide bonds that not only involved the mutant proinsulin, but also the co-expressed WT-proinsulin, forming misfolded disulfide-linked proinsulin complexes. This impaired the intracellular trafficking of WT-proinsulin and limited the production of bioactive mature insulin. Notably, although all three mutants presented with similar defects in folding, trafficking, and dominant negative behavior, the degrees of these defects appeared to be different. Specifically, compared to MODY mutants G(B20)R and P(B28)L that partially affected folding and trafficking of co-expressed WT-proinsulin, the NDM mutant C(A7)Y resulted in an almost complete blockade of the ER export of WT-proinsulin, decreasing insulin production, inducing more severe ER stress and apoptosis. We thus demonstrate that differences in cell biological behaviors among different proinsulin mutants correlate with the spectrum of diabetes phenotypes caused by the different INS gene mutations.
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http://dx.doi.org/10.1016/j.mce.2020.111025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734662PMC
December 2020

Spatial distribution, risk assessment and influence factors of terrestrial gamma radiation dose in China.

J Environ Radioact 2020 Oct 17;222:106325. Epub 2020 Jun 17.

School of Chemistry and Chemical Engineering, Zhoukou Normal University, Zhoukou, 466001, China.

The current spatial distribution of the risk of terrestrial gamma radiation in China were investigated by using spatial interpolation. And the driving factors influence on the terrestrial gamma radiation dose (TGRD) distribution were identified using the geographic detector, a new statistical method based on the nonlinear hypothesis. The results showed that the values of TGRD were range from 60 to 195 nGy h with the average of 86.5 nGy h, and the higher values were recorded in Qingahi-Tibet Plateau, which were all within the range of background value in China. In addition, the radiological indices, ELCR (Excess Lifetime Cancer Risk), TGRD and AEDE (Annual Effective Dose Equivalent) were also within the acceptable range of values by risk assessment. The results by use of the geographic detector showed that sunshine duration, atmosphere pressure, altitude, and rainfall condition have closely related to the TGRD distribution. In addition, these meteorological factors and altitude had more impact on TGRD than the air pollution-related factors. Our study can provide useful information on studying the influence mechanism of the TGRD distribution, the variability of the natural terrestrial gamma radiation in China, and exposure data for risk assessment from low dose chronic exposures.
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http://dx.doi.org/10.1016/j.jenvrad.2020.106325DOI Listing
October 2020

Monocyte-Derived Leukemia-Associated Macrophages Facilitate Extramedullary Distribution of T-cell Acute Lymphoblastic Leukemia Cells.

Cancer Res 2020 09 10;80(17):3677-3691. Epub 2020 Jul 10.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.

Macrophages play important roles in both physiologic and pathologic processes and arise from successive waves of embryonic and adult hematopoiesis. Monocyte-derived macrophages (MOMF) exert distinct functions under pathologic conditions, and leukemia-associated macrophages (LAM) show considerable diversities in activation and functional phenotype. However, their origin and pathologic roles have not been well elucidated. Here we used wild-type and CCR2 mice to study the pathologic roles of monocyte-derived LAM in extramedullary tissues in models of Notch1-induced T-cell acute lymphoblastic leukemia (T-ALL). MOMF existed in the resting liver and spleen. In the spleen, Ly6C monocytes gave rise to the Ly6C macrophage subset. Furthermore, an increase of monocyte-derived LAM, including the Ly6C subset, was detected in the extramedullary tissues in leukemic mice. More monocyte-derived LAM, including Ly6C LAM, was detected in the spleens of leukemic mice transplanted with exogeneous mononuclear cells. Moreover, Ly6C LAM exhibited increased M1-related characteristics and contributed to sterile inflammation. In CCR2 leukemic mice, reduced Ly6C LAM, relieved sterile inflammation, and reduced distribution of leukemia cells were detected in extramedullary tissues. In addition, monocyte-derived Ly6C LAM expressed high levels of CCL8 and CCL9/10. Blocking CCR1 and CCR2 relieved hepatosplenomegaly and inhibited the extramedullary distribution of leukemia cells in T-ALL mice. Collectively, our findings reveal the multifaceted pathologic roles of monocyte-derived LAM in T-ALL progression. SIGNIFICANCE: This study links monocyte-derived leukemia-associated macrophages with noninfectious inflammation and extramedullary distribution of leukemia cells during leukemia progression, providing new insight into macrophage-based immunotherapy in leukemia.
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http://dx.doi.org/10.1158/0008-5472.CAN-20-0034DOI Listing
September 2020

A dynamic model to evaluate the critical loads of heavy metals in agricultural soil.

Ecotoxicol Environ Saf 2020 Jul 15;197:110607. Epub 2020 Apr 15.

School of Metallurgy and Environment, Central South University, Changsha, 410083, China.

Estimation of critical load (CL) is important for soil environmental management and pollution prevention. We developed a mass balance-based dynamic critical load (DCL) model, which improved the model performance, applicability and functionality compared with the traditional one. Paddy soils in two typical fields in central south China and two scenarios were chosen as case studies. The result of case study showed that atmospheric deposition was the main source of Cd, Cu, Pb, and Zn in the soils, with percentage contributions ranging from 59.9 to 79.8%. Crop uptake, particularly the rice straw harvest, was the primary output pathway, accounting for 35.1-71.2% of the total output flux. The critical loads also known as annual input limits (I) of heavy metals in the paddy soils were calculated by the developed DCL model. For example, the Imax of Cd was recommended as 0.05 kg ha in the paddy soils under the default scenario for a protection period of 40 years, and that became 0.12 kg ha and 0.17 kg ha under the straw removal scenario in the two typical fields, respectively. The scenario simulation suggested that the straw removal strategy reduced the total concentrations of heavy metals (C) in the soils and notably increased the I. Meanwhile, the sensitivity analysis indicated that the changes of C and I can be controlled by adjusting the partition coefficient (K), plant uptake factor (PUF) and input flux. The mass balance-based DCL model provides a reference method to establish the standard for controlling heavy metal inputs to agricultural soil, this will be helpful to develop strategies for the prevention of soil contamination.
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http://dx.doi.org/10.1016/j.ecoenv.2020.110607DOI Listing
July 2020

Induction of CML-specific immune response through cross-presentation triggered by CTP-mediated BCR-ABL-derived peptides.

Cancer Lett 2020 07 10;482:44-55. Epub 2020 Apr 10.

Department of Clinical Hematology, Key Laboratory of Laboratory Medical Diagnostics Designated By Ministry of Education, School of Laboratory Medicine, Chongqing Medical University, Chongqing, China. Electronic address:

Although targeted therapy using tyrosine kinase inhibitors (TKIs) has made remarkable progress in treating chronic myeloid leukemia (CML), this disease remains largely incurable, warranting further investigation of new therapeutic strategies. BCR-ABL is a highly specific tumor antigen in CML and provides an attractive opportunity for vaccination therapy. Exogenous antigens must be presented on MHC class I molecules-via a process termed cross-presentation-to activate specific cytotoxic T lymphocyte response. The relative efficiency of cross-presentation is determined in part by the ability of dendritic cells (DCs) to internalize and present antigens. Here, we present a novel tool that uses cytoplasmic transduction peptide (CTP) to facilitate the internalization of antigens by DCs in an endocytosis-independent manner, which greatly enhances the efficiency of antigen presentation, thereby inducing stronger cytotoxic activity to ensure the elimination of CML cells. The data suggest that CTP-fused CML-specific peptides can be applied in vaccination therapies for CML patients.
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http://dx.doi.org/10.1016/j.canlet.2020.04.010DOI Listing
July 2020

Green synthesis of a novel Mn-Zn ferrite/biochar composite from waste batteries and pine sawdust for Pb removal.

Chemosphere 2020 Aug 19;252:126529. Epub 2020 Mar 19.

School of Petroleum and Environmental Engineering, Yan'an University, Yan'an, 716000, PR China.

Magnetic ferrite/biochar composites are a kind of promising adsorbents due to their high adsorption efficiency and facile magnetic separation; however, their synthesis is associated with high cost and secondary environmental impacts. In this study, a novel Mn-Zn ferrite/biochar composite (MZF-BC) is synthesized via a green two-step biocheaching and hydrothermal method using waste batteries and pine sawdust. Characterization results indicate that the introduced Mn-Zn ferrite particles are successfully embedded and coated on biochar (BC), and synthesized MZF-BC with 50% BC content exhibits best performance with a specific surface area of 138.5 m g, the saturation magnetization of 27.5 emu g and CEC value of 53.2 mmol 100 g. The maximum adsorption capacity of Pb is 99.5 mg g based on the Langmuir sorption isotherm study at 298 K, and pseudo-second-order model accurately describes the adsorption process. Regeneration test suggests that MZF-BC can be efficiently reused for 6 cycles. In addition, it exhibits a good selective Pb and Cd removal performance in lead-acid battery wastewater. The results illustrate that this newly developed material has low cost and rapid remediation of Pb as good application potential.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126529DOI Listing
August 2020

P2X7 promotes the progression of MLL-AF9 induced acute myeloid leukemia by upregulation of Pbx3.

Haematologica 2021 05 1;106(5):1278-1289. Epub 2021 May 1.

Institute of Hematology and Blood Diseases Hospital, Peking Union Medical College, China.

Nucleotides mediate intercellular communication by activating purinergic receptors and take part in various physiological and pathological processes. Abnormal purinergic signaling plays important roles in malignant progression. P2X7, which belongs to the P2X family of purinergic receptors, is abnormally expressed in various types of malignancies including leukemia. However, its role and molecular mechanism in leukemia have not been elucidated. Here, we analyzed the correlation between P2X7 expression and AML clinical outcome; explored the role and mechanism of P2X7 in AML progression by using mouse acute myeloid leukemia (AML), nude mouse xenograft and patient-derived xenograft models. High levels of P2X7 expression were correlated with worse survival in AML. P2X7 was highly expressed in MLL-rearranged AML. Furthermore, P2X7 accelerated the progression of MLL-rearranged AML by both promoting cell proliferation and increasing leukemia stem cell (LSC) levels. Moreover, P2X7 caused upregulation of Pbx3 accounts for its pro-leukemic effects. The P2X7-Pbx3 pathway might also contribute to the progression of other types of leukemia as well as solid tumors with high levels of P2X7 expression. Our study provides new insights into the malignant progression caused by abnormal purinergic signaling.
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http://dx.doi.org/10.3324/haematol.2019.243360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094107PMC
May 2021

PdIrBP mesoporous nanospheres combined with superconductive carbon black for the electrochemical determination and collection of circulating tumor cells.

Mikrochim Acta 2020 03 11;187(4):216. Epub 2020 Mar 11.

Department of Laboratory Medicine, Key Laboratory of Medical Diagnostics of Ministry of Education, Chongqing Medical University, No. 1 Yi Xue Yuan Road, Chongqing, 400016, People's Republic of China.

An integrated electrochemical immunoassay is described for the determination of circulating tumor cells (CTCs). For the first time, Ketjen black (KB), which is a superconductive carbon material, was incorporated with Au nanoparticles (AuNPs) and used to modify the surface of gold electrodes. A cocktail of anti-epithelial cell adhesion molecules (EpCAM) and anti-vimentin antibodies was chosen to capture the CTCs. Palladium-iridium-boron-phosphorus alloy-modified mesoporous nanospheres (PdIrBPMNS) served as a catalytic tag to amplify the current signal. Glycine-HCl (Gly-HCl) was used as an antibody eluent to release and collect the captured CTCs from the electrodes for further clinical research without compromising cell viability. The response of the method increased linearly from 10 to 1 × 10 cells mL CTCs, while the detection limit was calculated to be as low as 2 cells mL. This method was successfully used to determine CTCs in spiked blood samples and demonstrated good recovery. Graphical abstractKetjen black/AuNPs was incorporated in the electrochemical platform to enhance the electron transfer ability of the electrode surface. PdIrBP mesoporous nanospheres were used to amplify DPV signal in this assay. The introduction of Gly-HCl realized nondestructive recovery of circulating tumor cells.
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http://dx.doi.org/10.1007/s00604-020-4213-zDOI Listing
March 2020

Effects of Solid Acid Supports on the Bifunctional Catalysis of Levulinic Acid to γ-Valerolactone: Catalytic Activity and Stability.

Chem Asian J 2020 Apr 10;15(8):1182-1201. Epub 2020 Mar 10.

School of Environmental Science and Engineering, Tianjin University, Tianjin, 300350, P.R. China.

Heterogeneous transformation of levulinic acid (LA) to γ-valerolactone (GVL) is regarded as a critical process of the lignocellulose-based biorefinery system. Substantial progress on the catalytic conversion of LA to GVL has been continuously achieved recently. However, the traditional research paradigm typically emphasizes the metal-catalyzed hydrogenation step, but lacks profound insights into the potential impacts of catalyst supports. Herein, an overview of the bifunctional catalytic system classified by representative solid acid supports for LA conversion to GVL is presented, and effects of critical factors on metal- and acid- catalyzed processes are discussed. Particularly, impacts of key issues on catalytic stability are thoroughly summarized and analyzed. Challenges and suggestions are also proposed from the perspective of increases in both catalytic activity and stability. This review potentially contributes to the rational design of high-efficiency catalysts used in the biomass valorization for renewable energy production.
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http://dx.doi.org/10.1002/asia.202000006DOI Listing
April 2020
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