Publications by authors named "Wenjun Zhou"

226 Publications

Visible-light-promoted direct C3-trifluoromethylation and perfluoroalkylation of imidazopyridines.

Org Biomol Chem 2021 Sep 21. Epub 2021 Sep 21.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.

An efficient method for direct trifluoromethylation and perfluoroalkylation at C3 of imidazopyridines through visible light-promoted C-H bond functionalization was developed. Under the irradiation of a blue LED, a series of C3-perfluoroalkyl-substituted imidazopyridines were synthesized from the corresponding imidazopyridines and perfluoroalkyl iodides in moderate to good yields at room temperature. It should be mentioned that this reaction proceeded in the absence of any transition-metal catalyst, oxidant and photocatalyst.
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http://dx.doi.org/10.1039/d1ob01417jDOI Listing
September 2021

Altered albedo dominates the radiative forcing changes in a subtropical forest following an extreme snow event.

Glob Chang Biol 2021 Sep 15. Epub 2021 Sep 15.

Indian Institute of Tropical Meteorology, Ministry of Earth Sciences, Pune, 411008, India.

Subtropical forests are important ecosystems globally due to their extensive role in carbon sequestration. Extreme climate events are known to introduce disturbances in the ecosystem that cause long-term changes in carbon balance and radiation reflectance. However, how these ecosystem function changes contribute to global warming in terms of radiative forcing (RF), especially in the years following a disturbance, still needs to be investigated. We studied an extreme snow event that occurred in a subtropical evergreen broad-leaved forest in southwestern China in 2015 and used 9 years (2011-2019) of net ecosystem CO exchange (NEE) and surface albedo (α) data to investigate the effect of the event on the ecosystem RF changes. In the year of the disturbance, leaf area index (LAI) declined by 40% and α by 32%. The annual NEE was -718 ± 128 gC m as a sink in the pre-disturbance years (2011-2014), but after the event, the sink strength dropped significantly by 76% (2015). Both the vegetation, indicated by LAI, and α recovered to pre-disturbance levels in the fourth post-disturbance year (2018). However, the NEE recovery lagged and occurred a year later in 2019, suggesting a more severe and lasting impact on the ecosystem carbon balance. Overall, the extreme event caused a positive (warming effect) net RF which was predominantly caused by changes in α (90-93%) rather than those in NEE. This result suggests that, compared to the climate effect caused by forest carbon sequestration changes, the climate effect of α alterations can be more sensitive to vegetation damage induced by natural disturbances. Moreover, this study demonstrates the important role of vegetation recovery in driving canopy reflectance and ecosystem carbon balance during the post-disturbance period, which determines the ecosystem feedbacks to the climate change.
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http://dx.doi.org/10.1111/gcb.15885DOI Listing
September 2021

Multi-exposure interferometric diffusing wave spectroscopy.

Opt Lett 2021 Sep;46(18):4498-4501

We present multi-exposure interferometric diffusing wave spectroscopy (MiDWS), which measures brain blood flow index (BFI) continuously and non-invasively. MiDWS employs interferometry to detect low light levels, probing the optical field autocorrelation indirectly by varying the sensor exposure time. Here MiDWS is compared with conventional interferometric diffusing wave spectroscopy and speckle contrast optical spectroscopy in phantoms. Notably, the MiDWS approach enables the use of low frame rate, two-dimensional complementary metal-oxide semiconductor cameras in a short exposure time regime, where detector noise greatly exceeds the sample photon count. Finally, we show that MiDWS can monitor the BFI simultaneously at two source-collector separations (1 and 3 cm) on the adult human head on a single camera, enabling the use of superficial signal regression techniques to improve brain specificity.
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http://dx.doi.org/10.1364/OL.427746DOI Listing
September 2021

Examining gender and the longitudinal effect of weight conscious drinking dimensions on body mass index among a college freshman cohort.

J Am Coll Health 2021 Sep 1:1-9. Epub 2021 Sep 1.

Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida, USA.

Objective: This study aims to: (1) examine gender differences for weight conscious drinking among college students accounting for the broader phenomenon (e.g. including the Alcohol Effects dimension); and (2) longitudinally examine the effect of weight conscious drinking behaviors on body mass index (BMI). United States freshmen students from eight participating universities (N= 1,149). Structural equation modeling was used to model the effect of gender on weight conscious drinking dimensions at 7-month follow-up. Findings suggest a significant effect of gender on Alcohol Effects (β = -.15, SE = .05,  = .005) at 7-month follow-up among college freshmen. Weight conscious drinking dimensions predicted no significant change in BMI at 7-month follow-up among college freshmen. Findings contribute to weight conscious drinking theory and provide campus weight conscious drinking prevention initiatives with evidence to tailor their programming to address female tendencies to engage in compensatory strategies to enhance the psychoactive effects of alcohol.
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http://dx.doi.org/10.1080/07448481.2021.1943410DOI Listing
September 2021

From Unimolecular Template to Silver Nanocrystal Clusters: An Effective Strategy to Balance Antibacterial Activity and Cytotoxicity.

ACS Appl Mater Interfaces 2021 Aug 13;13(33):39806-39818. Epub 2021 Aug 13.

School of Materials Science and Engineering, Henan Key Laboratory of Advanced Nylon Materials and Application, Zhengzhou University, Zhengzhou 450001, China.

Silver nanomaterials have attracted a great deal of interest due to their broad-spectrum antimicrobial activity. However, it is still challenging to balance the high antibacterial efficiency with low damage to biological cells of silver nanostructures, especially when the diameter decreases to less than 10 nm. Here, we developed a new type of Ag nanohybrid material via a unimolecular micelle template method, which presents amazing antibacterial activities and almost noncytotoxicity. First, water-soluble multiarm star-shaped brushlike copolymer α-CD--[(PEO--PAA)--PEO] was precisely synthesized and its micelle behavior in different solvents was revealed. Then, nanocrystal clusters assembled by Ag grains ([email protected] NCs) were prepared through an in situ redox route using the unimolecular micelle of α-CD--[(PEO--PAA)--PEO] as the soft template, AgNO as a precursor, and tetrabutylammonium borohydride (TBAB) as the reducing agent. The overall size of the achieved [email protected] NCs is controlled by the template structure at around 40 nm ( in DMF), and the size of the Ag grain can be easily regulated from ∼1 to ∼5 nm by adjusting the feeding ratio of AgNO/acrylic acid (AA) units in the template from 1:10 to 1:1. Benefitting from the structural design of the template, all [email protected] NCs prepared here exhibit excellent dispersibility and chemical stability in different aqueous environments (neutral, pH = 5.5, and 0.9% NaCl physiological saline solution), which play a crucial role in the long-term storage and potential application in a complex physiological environment. The antibacterial and cytotoxicity tests indicate that [email protected] NCs display much better performance than Ag nanoparticles (Ag NPs), which have a comparable overall size of ∼25 nm. The inhibitory capability of [email protected] NCs to bacteria strongly depends on the grain size. Specifically, the [email protected] NC assembled by the smallest grains (1.6 ± 0.3 nm) presents the best antibacterial activity. For (-), the MIC value is as low as 5 μg/mL (0.36 μg/mL of Ag), while for (+), the value is around 10 μg/mL (0.72 μg/mL of Ag). The survival rate of L02 cells and lactate dehydrogenase assay together illustrate the low cytotoxicity possessed by the prepared [email protected] NCs. Therefore, the proposed [email protected] NC structure successfully resolves the high reactivity, instability, and fast oxidation issues of the ultrasmall Ag nanoparticles, and integrates high antibacterial efficiency and nontoxicity to biological cells into one platform, which implies its broad potential application in biomedicine.
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http://dx.doi.org/10.1021/acsami.1c07986DOI Listing
August 2021

FHL1 Inhibits the Progression of Colorectal Cancer by Regulating the Wnt/β-Catenin Signaling Pathway.

J Cancer 2021 3;12(17):5345-5354. Epub 2021 Jul 3.

Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

This study aims to explore the FHL1 expression level in colorectal cancer (CRC) patients, analyze its association with patient survival and investigate the role of FHL1 in CRC. We used secondary sequencing to profile mRNA expression in CRC tissue and corresponding adjacent normal tissue from four CRC patients. We focus on FHL1 and analyzed the association between its expression level and clinical indicators. Furthermore, we explored the functional role of FHL1 in colorectal cancer tumorigenesis by transfecting cells with siRNA or overexpression plasmids. Hierarchical clustering revealed significantly differentially expressed mRNAs. FHL1 expression was significantly lower in CRC tissue than in adjacent normal tissue as well as in CRC cell lines relative to NCM460. Low FHL1 expression in CRC tissue correlated with poor patient survival. Our data demonstrated that overexpression of FHL1 inhibited the proliferation, colony formation potential, and expression of CdK4 and Cyclin D1, whereas ablating FHL1 promoted their proliferation and colony formation potential, suggesting that FHL1 acts as a tumor suppressor in CRC. Moreover, we showed that FHL1 inhibited the proliferation of colorectal cancer cells by negatively regulating the Wnt/β-catenin signaling pathway. FHL1 is a potential tumor suppressor gene in colorectal cancer, and regulation of the FHL1-Wnt/β-catenin pathway may be part of its antitumor mechanism.
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http://dx.doi.org/10.7150/jca.60543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317513PMC
July 2021

Recent Advance in Ionic-Liquid-Based Electrolytes for Rechargeable Metal-Ion Batteries.

Adv Sci (Weinh) 2021 Jul 2;8(13):2004490. Epub 2021 May 2.

Department of Materials Science and Engineering City University of Hong Kong Hong Kong 999077 China.

From basic research to industry process, battery energy storage systems have played a great role in the informatization, mobility, and intellectualization of modern human society. Some potential systems such as Li, Na, K, Mg, Zn, and Al secondary batteries have attracted much attention to maintain social progress and sustainable development. As one of the components in batteries, electrolytes play an important role in the upgrade and breakthrough of battery technology. Since room-temperature ionic liquids (ILs) feature high conductivity, nonflammability, nonvolatility, high thermal stability, and wide electrochemical window, they have been widely applied in various battery systems and show great potential in improving battery stability, kinetics performance, energy density, service life, and safety. Thus, it is a right time to summarize these progresses. In this review, the composition and classification of various ILs and their recent applications as electrolytes in diverse metal-ion batteries (Li, Na, K, Mg, Zn, Al) are outlined to enhance the battery performances.
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http://dx.doi.org/10.1002/advs.202004490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261505PMC
July 2021

Expression of miR-451a in Prostate Cancer and Its Effect on Prognosis.

Iran J Public Health 2021 Apr;50(4):772-779

Department of Urology, Changshu Hospital Affiliated to Soochow University, Changshu No.1 People's Hospital, Changshu 215500, P.R. China.

Background: To investigate the expression of miR-451a in prostate cancer tissues and its effect on prognosis.

Methods: Each of 78 specimens of prostate cancer tissues and corresponding adjacent normal tissues were collected from patients in Changshu Hospital Affiliated to Soochow University, Changshu, China from Apr 2014 to Jun 2015. Real-time quantitative RT-PCR (qRT-PCR) was used to detect the expression of miR-451a in tissues. The relationship between the expression of miR-451a and clinical pathological parameters was analyzed. The median expression of miR-451a in the experimental group was used to distinguish the high and low expressions of miR-451a in the experimental group. Kaplan-Meier was used to analyze the survival of miR-451a high and low expression groups.

Results: The expressions of miR-451a in the patient's tissues and serum were decreased, and the correlation analysis found that they were positively correlated. ROC curve analysis showed that miR-451a had a high clinical value in the diagnosis of prostate cancer and the area under the curve was 0.921. The incidence of stage III+IV lymph node metastasis, Gleason score of >7 points and a serum Prostate-specific antigen (PSA) level of >20 ng/ml in patients of the low expression group increased significantly. The 5-yr survival rate of patients with low expression was significantly lower than that of those with high expression (=0.005). MiR-451a was an independent factor affecting the prognosis of patients.

Conclusion: miR-451a is lowly expressed in prostate cancer, and patients with low expression have a poor prognosis.
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http://dx.doi.org/10.18502/ijph.v50i4.6002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219609PMC
April 2021

Quantitative Profiling of Oxylipin Reveals the Mechanism of Pien-Tze-Huang on Alcoholic Liver Disease.

Evid Based Complement Alternat Med 2021 1;2021:9931542. Epub 2021 Jun 1.

Institute of Digestive Diseases, Longhua Hospital, China-Canada Center of Research for Digestive Diseases (ccCRDD), Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

Alcoholic liver disease (ALD) is a liver disease caused by long-term alcohol consumption. ROS-mediated oxidative stress is the leading cause of ALD. Pien-Tze-Huang (PZH), a traditional formula, is famous in China. This study was designed to evaluate the effects and explore the potential mechanisms of PZH in ALD. Forty mice were randomly divided into five groups: control group (normal diet + vehicle), model group (ethanol diet + vehicle), PZH-L group (ethanol diet + PZH (0.125 g/kg)), PZH-M group (ethanol diet + PZH (0.25 g/kg)), and PZH-H group (ethanol diet + PZH (0.5 g/kg)). The mice were sacrificed, and their liver and blood samples were preserved. Liver steatosis, triglyceride (TG), total cholesterol, serum alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels were assayed. Malondialdehyde (MDA), glutathione peroxidase (GSH-PX), and total superoxide dismutase were identified using commercial kits. Oxylipins were profiled, and the data were analyzed. The AMPK/ACC/CPT1A pathway was identified using real-time polymerase chain reaction and western blotting. The PZH-H intervention significantly alleviated hepatic steatosis and injury and reduced the levels of liver TG and serum ALT and AST. In addition, MDA levels were markedly reduced, and GSH-PX activity significantly increased after PZH-H intervention. Finally, PZH-H increased the levels of 17-HETE, 15-HEPE, 9-HOTrE, 13-HOTrE, and 5,6-dihydroxy-8Z,11Z,14Z,17Z-eicosatetraenoic acid, and reduced PGE2 levels. PZH-H intervention also promoted the phosphorylation of AMPK and ACC, and the expression of CPT1A. In conclusion, PZH reduced oxidative stress and alleviated hepatic steatosis and injury. The mechanism was correlated with the oxylipin metabolites/AMPK/ACC/CPT1A axis.
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http://dx.doi.org/10.1155/2021/9931542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187045PMC
June 2021

The Role of Alcohol-Related Proactive Dietary Restriction to Feel the Psychoactive Effects of Alcohol Faster on Binge Drinking Frequency among Freshmen College Students.

Subst Use Misuse 2021 19;56(9):1266-1274. Epub 2021 May 19.

Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida, USA.

Background: There is a positive cross-sectional relationship between alcohol-related proactive dietary restriction to feel the effects of alcohol faster (APDR) and binge drinking, a health and safety issue impacting college students. To examine: 1) the longitudinal predictive ability of varying levels of APDR on binge drinking frequency; and 1a) the strength of the relationship between varying levels of APDR and binge drinking frequency during freshman year of college ( = 1,149).

Methods: Ordinal logistic regression was used to model the relationship between APDR and binge drinking frequency.

Results: Main findings suggest APDR of students who reported eating less than usual (low APDR) prior to drinking to feel the effects of alcohol faster was a significant predictor of binge drinking frequency (1.27 (95% CI, 0.06 to 0.42), Wald (1) = 8.46, =.009) at baseline, but not at 7-month follow-up (1.02 (95% CI, -0.18 to 0.23), Wald (1) = .51, =.83). APDR for students who reported skipping one or more meals (high APDR) to feel the effects of alcohol faster was not a significant predictor of binge drinking frequency at baseline nor at 7-month follow-up.

Conclusion: Low APDR is a significant predictor of binge drinking frequency that is established early in the first semester of college with no significant change occurring in binge drinking frequency over the course of students' freshman year at 7-month follow-up. Campus health professionals are urged to emphasize the detrimental health effects of low APDR early in the first semester of college.
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http://dx.doi.org/10.1080/10826084.2021.1914104DOI Listing
August 2021

Functional interferometric diffusing wave spectroscopy of the human brain.

Sci Adv 2021 May 12;7(20). Epub 2021 May 12.

Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA.

Cerebral blood flow (CBF) is essential for brain function, and CBF-related signals can inform us about brain activity. Yet currently, high-end medical instrumentation is needed to perform a CBF measurement in adult humans. Here, we describe functional interferometric diffusing wave spectroscopy (fiDWS), which introduces and collects near-infrared light via the scalp, using inexpensive detector arrays to rapidly monitor coherent light fluctuations that encode brain blood flow index (BFI), a surrogate for CBF. Compared to other functional optical approaches, fiDWS measures BFI faster and deeper while also providing continuous wave absorption signals. Achieving clear pulsatile BFI waveforms at source-collector separations of 3.5 cm, we confirm that optical BFI, not absorption, shows a graded hypercapnic response consistent with human cerebrovascular physiology, and that BFI has a better contrast-to-noise ratio than absorption during brain activation. By providing high-throughput measurements of optical BFI at low cost, fiDWS will expand access to CBF.
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http://dx.doi.org/10.1126/sciadv.abe0150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115931PMC
May 2021

Salvia-Nelumbinis naturalis extract protects mice against MCD diet-induced steatohepatitis via activation of colonic FXR-FGF15 pathway.

Biomed Pharmacother 2021 Jul 14;139:111587. Epub 2021 Apr 14.

Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. Electronic address:

Salvia-Nelumbinis naturalis (SNN) formula is a traditional Chinese medicine prescription, and has been confirmed to be effective in treating non-alcoholic steatohepatitis (NASH), but the underlying mechanisms are still unknown. Here we showed that 4-week SNN administration alleviated methionine-choline-deficiency (MCD) diet-induced hepatic steatosis and inflammation as well as serum levels of alanine transaminase (ALT) increase in C57BL/6 mice. Fecal 16S rDNA sequencing indicated that SNN altered the structure of gut microbiota and partially reversed the gut dysbiosis. Simultaneously, we analyzed the fecal BA profile using liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-TQMS) -based metabolomics, and found that SNN modulated fecal BA profile, predominantly increased the microbiomes related BA species (e.g. nordeoxycholic acid) which in turn, activated farnesoid X receptor (FXR)-fibroblast growth factor 15 (FGF15) signaling pathway in the colon but not the ileum. The activation of intestinal FXR-FGF15 signaling was accompanied by increase of liver protein kinase B (PKB/Akt) phosphorylation, and decrease of p-65 subunit of NF-κB phosphorylation, resulting in less liver CD68 positive macrophages, and inflammatory cytokine IL-1β and TNF-α expression. Our results established the link between SNN treatment, gut microbiota, BA profile and NASH, which might shed light into the mechanisms behind the beneficial effects of SNN on NASH, thus provide evidence for the clinical application of SNN.
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http://dx.doi.org/10.1016/j.biopha.2021.111587DOI Listing
July 2021

Inter- and Transgenerational Effects of Paternal Exposure to Inorganic Arsenic.

Adv Sci (Weinh) 2021 04 18;8(7):2002715. Epub 2021 Feb 18.

Division of Endocrinology Department of Medicine Baylor College of Medicine Houston TX 77030 USA.

The rise of metabolic disorders in modern times is mainly attributed to the environment. However, heritable effects of environmental chemicals on mammalian offsprings' metabolic health are unclear. Inorganic arsenic (iAs) is the top chemical on the Agency for Toxic Substances and Disease Registry priority list of hazardous substances. Here, we assess cross-generational effects of iAs in an exclusive male-lineage transmission paradigm. The exposure of male mice to 250 ppb iAs causes glucose intolerance and hepatic insulin resistance in F1 females, but not males, without affecting body weight. Hepatic expression of glucose metabolic genes, glucose output, and insulin signaling are disrupted in F1 females. Inhibition of the glucose 6-phosphatase complex masks the intergenerational effect of iAs, demonstrating a causative role of hepatic glucose production. F2 offspring from grandpaternal iAs exposure show temporary growth retardation at an early age, which diminishes in adults. However, reduced adiposity persists into middle age and is associated with altered gut microbiome and increased brown adipose thermogenesis. In contrast, F3 offspring of the male-lineage iAs exposure show increased adiposity, especially on a high-calorie diet. These findings have unveiled sex- and generation-specific heritable effects of iAs on metabolic physiology, which has broad implications in understanding gene-environment interactions.
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http://dx.doi.org/10.1002/advs.202002715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025034PMC
April 2021

REV-ERB in GABAergic neurons controls diurnal hepatic insulin sensitivity.

Nature 2021 Apr 24;592(7856):763-767. Epub 2021 Mar 24.

Department of Medicine, Division of Diabetes, Endocrinology and Metabolism, Baylor College of Medicine, Houston, TX, USA.

Systemic insulin sensitivity shows a diurnal rhythm with a peak upon waking. The molecular mechanism that underlies this temporal pattern is unclear. Here we show that the nuclear receptors REV-ERB-α and REV-ERB-β (referred to here as 'REV-ERB') in the GABAergic (γ-aminobutyric acid-producing) neurons in the suprachiasmatic nucleus (SCN) (SCN neurons) control the diurnal rhythm of insulin-mediated suppression of hepatic glucose production in mice, without affecting diurnal eating or locomotor behaviours during regular light-dark cycles. REV-ERB regulates the rhythmic expression of genes that are involved in neurotransmission in the SCN, and modulates the oscillatory firing activity of SCN neurons. Chemogenetic stimulation of SCN neurons at waking leads to glucose intolerance, whereas restoration of the temporal pattern of either SCN neuron firing or REV-ERB expression rescues the time-dependent glucose metabolic phenotype caused by REV-ERB depletion. In individuals with diabetes, an increased level of blood glucose after waking is a defining feature of the 'extended dawn phenomenon'. Patients with type 2 diabetes with the extended dawn phenomenon exhibit a differential temporal pattern of expression of REV-ERB genes compared to patients with type 2 diabetes who do not have the extended dawn phenomenon. These findings provide mechanistic insights into how the central circadian clock regulates the diurnal rhythm of hepatic insulin sensitivity, with implications for our understanding of the extended dawn phenomenon in type 2 diabetes.
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http://dx.doi.org/10.1038/s41586-021-03358-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085086PMC
April 2021

Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.

J Cell Physiol 2021 Oct 8;236(10):6948-6962. Epub 2021 Mar 8.

Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.

ACSS1/2 converts acetate into acetyl-coenzyme A, which contributes to histone acetylation in the mitochondria and cytoplasm. Zygotic genome activation (ZGA) is critical for embryo development involving drastic histone modification. An efficient crRNAs-Cas13a targeting strategy was employed to investigate the ACSS1/2 function during ZGA. The results showed that nuclear accumulation of ACSS1 and ACSS2 occurs during ZGA. Knockdown of ACSS1/2 did not affect blastocyst formation when using a normal medium. On culturing embryos in a medium with acetate and no pyruvate (-P + Ace), knockdown of ACSS1 did not affect histone acetylation levels but significantly reduced ATP levels, whereas knockdown of ACSS2 significantly reduced histone acetylation levels in porcine embryos. Inhibition of fatty acid beta-oxidation by etomoxir significantly reduced ATP levels, which could be restored by acetate. The histone acetylation levels in the ACSS1 and ACSS2 knockdown groups both decreased considerably after etomoxir treatment. Moreover, acetate showed dose-dependent effects on SIRT1 and SIRT3 levels when under metabolic stress. The C-terminus of ACSS1 regulated the nuclear translocation. In conclusion, ACSS1/2 helps to maintain ATP and histone acetylation levels in porcine early embryos under metabolic stress during ZGA.
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http://dx.doi.org/10.1002/jcp.30355DOI Listing
October 2021

Topography-related controls on NO emission and CH uptake in a tropical rainforest catchment.

Sci Total Environ 2021 Jun 9;775:145616. Epub 2021 Feb 9.

Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303 Mengla, Yunnan, China; Center of Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, 666303 Xishuangbanna, Yunnan, China; University of Chinese Academy of Sciences, 100039 Beijing, China. Electronic address:

Forest soils in the warm-humid tropics significantly contribute to the regional greenhouse gas (GHG) budgets. However, spatial heterogeneity of GHG fluxes is often overlooked. Here, we present a study of NO and CH fluxes over 1.5 years, along a topographic gradient in a rainforest catchment in Xishuangbanna, SW China. From the upper hillslope to the foot of the hillslope, and further to the flat groundwater discharge zone, we observed a decrease of NO emission associated with an increase of soil water-filled-pore-space (WFPS), which we tentatively attribute to more complete denitrification to N at larger WFPS. In the well-drained soils on the hillslope, denitrification at anaerobic microsites or under transient water-saturation was the potential NO source. Negative CH fluxes across the catchment indicated a net soil CH sink. As the oxidation of atmospheric CH is diffusion-limited, soil CH consumption rates were negatively related to WFPS, reflecting the topographic control. Our observations also suggest that during dry seasons NO emission was significantly dampened (<10 μg NO-N m h) and CH uptake was strongly enhanced (83 μg CH-C m h) relative to wet seasons (17 μg NO-N m h and 56 μg CH-C m h). In a post-drought period, several rain episodes induced exceptionally high NO emissions (450 μg NO-N m h) in the groundwater discharge zone, likely driven by flushing of labile organic carbon accumulated during drought. Considering the global warming potential associated with both GHGs, we found that NO emissions largely offset the C sink contributed by CH uptake in soils (more significant in the groundwater discharge zone). Our study illustrates important topographic controls on NO and CH fluxes in forest soils. With projected climate change in the tropics, weather extremes may interact with these controls in regulating forest GHG fluxes, which should be accounted for in future studies.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145616DOI Listing
June 2021

Gut Microbiota and Related Metabolites Were Disturbed in Ulcerative Colitis and Partly Restored After Mesalamine Treatment.

Front Pharmacol 2020 18;11:620724. Epub 2021 Jan 18.

Institute of Digestive Diseases, China-Canada Center of Research for Digestive Diseases (ccCRDD), Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Mesalamine has been well used in the improvement of ulcerative colitis (UC) in clinics, however, the underlying mechanisms were not well illustrated. To explore its efficacy from the perspective of gut microbiota and related metabolites, we employed 16S rRNA sequencing and metabolomics approaches in stool samples across 14 normal healthy controls (NC group), 10 treatment-naïve UC patients (UC group) and 14 UC patients responded to mesalamine treatment (mesalamine group). We noted that the gut microbiota diversity and community composition were remarkably perturbed in UC group and partially restored by mesalamine treatment. The relative abundance of 192 taxa in genus level were significantly changed in UC group, and 168 genera were significantly altered after mesalamine intervention. Meanwhile, a total of 127 metabolites were significantly changed in UC group and 129 metabolites were significantly altered after mesalamine treatment. Importantly, we observed that many candidates including 49 genera (such as and ) and 102 metatoblites (such as isoleucine, cholic acid and deoxycholic acid) were reversed by mesalamine. Spearman correlation analysis revealed that most of the candidates were significantly correlated with Mayo score of UC, and the relative abundance of specific genera were significant correlated with the perturbation of metabolites. Pathway analysis demonstrated that genera and metabolites candidates were enriched in many similar molecular pathways such as amino acid metabolism and secondary metabolites biosynthesis. Importantly, ROC curve analysis identified a gut microbiota signature composed of five genera including and [] which might be used to distinguish UC group from both NC and mesalamine group. In all, our results suggested that mesalamine might exert a beneficial role in UC by modulating gut microbiota signature with correlated metabolites in different pathways, which may provide a basis for developing novel candidate biomarkers and therapeutic targets of UC.
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http://dx.doi.org/10.3389/fphar.2020.620724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898679PMC
January 2021

Therapeutic effect of Jianpi Liqi Fang combined with transcatheter arterial chemoembolization in patients with hepatocellular carcinoma and spleen deficiency syndrome.

J Tradit Chin Med 2021 02;41(1):157-166

Research Center for Traditional Chinese Medicine Complexity System, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Objective: To investigate the therapeutic effect of the Jianpi Liqi Fang ( ,JPLQF) combined with transcatheter arterial chemoembolization (TACE) in patients with hepatocellular carcinoma (HCC) and spleen deficiency syndrome (SDS) and identify a potential indicator of efficacy.

Methods: Ninety-nine patients with HCC who were diagnosed with SDS, non-spleen deficiency syndrome (NSDS), or no syndrome (NS) were treated with JPLQF combined with TACE for three periods. Therapeutic efficacy was compared among the groups. Plasma proteins were screened using label-free discovery analysis and verified via enzyme-linked immunosorbent assay (ELISA). Furthermore, receiver operating characteristic (ROC) curves were analyzed to evaluate therapeutic indicators.

Results: After treatment, the Karnofsky Performance Status was significantly improved in the SDS group and significantly better than that in the NS group. The Traditional Chinese Medicine (TCM) syndrome scores were lower in the SDS group after treatment and lower than those in the NSDS group. However, alanine aminotransferase, carbohydrate antigen 19-9, alpha-fetoprotein, and carcinoembryonic antigen levels and white blood cell and platelet counts did not differ among the groups. Serum aspartate aminotransferase levels in the SDS group were significantly lower after treatment than before treatment, and total bilirubin levels were significantly lower in the SDS group than in the NSDS group. Label-free analysis identified 24 differentially expressed proteins (DEPs) between the SDS and NS groups, including 17 and 7 upregulated and downregulated proteins, respectively. Fibulin-5 (FBLN5) displayed the largest difference in expression between the groups. ELISA confirmed that FBLN5 levels were significantly lower in the NSDS and NS groups than in the SDS group. Following treatment with JPLQF and TACE, FBLN5 expression was upregulated only in the SDS group. Furthermore, ROC curve analysis indicated that FBLN5 may serve as a potential indicator of the efficacy of JPLQF combined with TACE in patients with HCC and SDS.

Conclusion: JPLQF combined with TACE improved quality of life, clinical TCM symptoms, and liver function in patients with HCC and SDS. FBLN5 expression was significantly altered by treatment with JPLQF and TACE in patients with HCC and SDS.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2021.01.018DOI Listing
February 2021

AgRP neurons trigger long-term potentiation and facilitate food seeking.

Transl Psychiatry 2021 01 5;11(1):11. Epub 2021 Jan 5.

Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, 77030, USA.

Sufficient feeding is essential for animals' survival, which requires a cognitive capability to facilitate food seeking, but the neurobiological processes regulating food seeking are not fully understood. Here we show that stimulation of agouti-related peptide-expressing (AgRP) neurons triggers a long-term depression (LTD) of spontaneous excitatory post-synaptic current (sEPSC) in adjacent pro-opiomelanocortin (POMC) neurons and in most of their distant synaptic targets, including neurons in the paraventricular nucleus of the thalamus (PVT). The AgRP-induced sEPCS LTD can be enhanced by fasting but blunted by satiety signals, e.g. leptin and insulin. Mice subjected to food-seeking tasks develop similar neural plasticity in AgRP-innervated PVT neurons. Further, ablation of the majority of AgRP neurons, or only a subset of AgRP neurons that project to the PVT, impairs animals' ability to associate spatial and contextual cues with food availability during food seeking. A similar impairment can be also induced by optogenetic inhibition of the AgRP→PVT projections. Together, these results indicate that the AgRP→PVT circuit is necessary for food seeking.
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http://dx.doi.org/10.1038/s41398-020-01161-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791100PMC
January 2021

Synthesis of oxygen vacancy-enriched N/P co-doped CoFeO for high-efficient degradation of organic pollutant: Mechanistic insight into radical and nonradical evolution.

Environ Pollut 2021 Feb 13;270:116092. Epub 2020 Dec 13.

Department of Environmental Science, Zhejiang University, Hangzhou, Zhejiang 310058, China; The Key Laboratory of Organic Pollution Process and Control, Zhejiang Province, Hangzhou, Zhejiang 310058, China. Electronic address:

Oxygen vacancy-enriched N/P co-doped cobalt ferrite (NPCFO) was synthesized using ionic liquid as N and P sources, and then the catalytic performance and mechanism of NPCFO upon peroxymonosulfate (PMS) activation for the degradation of organic pollutants were investigated. The as-synthesized NPCFO-700 exhibited excellent catalytic performance in activating PMS, and the degradation rate constant of 4-chlorophenol (4-CP) increased with the increase of OV concentration in NPCFO-x. EPR analysis confirmed the existence of ·OH, SO, and O in the NPCFO-700/PMS system, in which OV could induce the generation of O by PMS adsorption and successive capture, and also served as electronic transfer medium to accelerate the redox cycle of M/M (M denotes Co or Fe) for the generation of radical to synergistically degrade organic pollutants. In addition, the contribution of free radical and nonradical to 4-CP degradation was observed to be strongly dependent on solution pH, and SO was the major ROS in 4-CP degradation under acid and alkaline condition, while O was involved in the degradation of 4-CP under neutral condition due its selective oxidation capacity, as evidenced by the fact that such organic pollutants with ionization potential (IP) below 9.0 eV were more easily attacked by O. The present study provided a novel insight into the development of transition metal-based heterogeneous catalyst containing massive OV for high-efficient PMS activation and degradation of organic pollutants.
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http://dx.doi.org/10.1016/j.envpol.2020.116092DOI Listing
February 2021

CircRNA_0000392 promotes colorectal cancer progression through the miR-193a-5p/PIK3R3/AKT axis.

J Exp Clin Cancer Res 2020 Dec 14;39(1):283. Epub 2020 Dec 14.

Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.

Background: Circular RNAs (circRNAs), important members of the noncoding RNA family, have been recently revealed to play a role in the pathogenic progression of diseases, particularly in the malignant progression of cancer. With the application of high-throughput sequencing technology, a large number of circRNAs have been identified in tumor tissues, and some circRNAs have been demonstrated to act as oncogenes. In this study, we analyzed the circRNA expression profile in colorectal cancer (CRC) tissues and normal adjacent tissues by high-throughput sequencing. We focused on circRNA_0000392, a circRNA with significantly increased expression in CRCtissues, and further investigated its function in the progression of colorectal cancer.

Methods: The expression profile of circRNAs in 6 pairs of CRC tissues and normal adjacent tissues was analyzed by RNA sequencing. We verified the identified differentially expressed circRNAs in additional samples by qRT-PCR and selected circRNA_0000392 to evaluate its associations with clinicopathological features. Then, we knocked down circRNA_0000392 in CRC cells and investigated the in vitro and in vivo effects using functional experiments. Dual luciferase and RNA pull-down assays were performed to further explore the downstream potential molecular mechanisms.

Results: CircRNA_0000392 was significantly upregulated in CRC compared with normal adjacent tissues and cell lines. The expression level of circRNA_0000392 was positively correlated with the malignant progression of CRC. Functional studies revealed that reducing the expression of circRNA_0000392 could inhibit the proliferation and invasion of CRC both in vitro and in vivo. Mechanistically, circRNA_0000392 could act as a sponge of miR-193a-5p and regulate the expression of PIK3R3, affecting the activation of the AKT-mTOR pathway in CRC cells.

Conclusions: CircRNA_0000392 functions as an oncogene through the miR-193a-5p/PIK3R3/Akt axis in CRC cells, suggesting that circRNA_0000392 is a potential therapeutic target for the treatment of colorectal cancer and a predictive marker for CRC patients.
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http://dx.doi.org/10.1186/s13046-020-01799-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7735421PMC
December 2020

Berberine compounds improves hyperglycemia via microbiome mediated colonic TGR5-GLP pathway in db/db mice.

Biomed Pharmacother 2020 Dec 1;132:110953. Epub 2020 Nov 1.

Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China. Electronic address:

Berberine compounds (BC), consisting of berberine (BBR), oryzanol and vitamin B, have been used to treat diabetes and hyperlipidemia in recent years, but the potential mechanisms under the effects have not been well determined. In this study, we evaluated the effect of BC in db/db mice, and found that BC treatment reversed the increased levels of fasting glucose and hemoglobin A1c in db/db mice, which was superior to BBR treatment. Fecal 16S rRNA gene sequencing indicated that BC increased relative abundance of microbiomes Bacteroidaceae and Clostridiaceae, which may promote conversion of primary bile acid cholic acid (CA) into secondary bile acid deoxycholic acid (DCA). Gas chromatography/mass spectrometry (GC/MS)-based metabolomics revealed that BC treatment increased fecal DCA level. Since DCA processes the potential to activate bile acid receptor-takeda G protein-coupled receptor 5 (TGR5) and induce glucagon-like peptide (GLP) secretion, we detected TGR5 expression, and found that BC-treatment significantly increased the colonic TGR5 and serum GLP-1/-2 levels in db/db mice. Modulation of TGR5-GLP pathway may also affect metabolomic profiles of serum and liver, and BC treatment showed effects on restoring the altered carbohydrate, lipid, amino acid and nucleotide metabolism. Our study suggested that BC improved hyperglycemia, the effect might attribute to the increased microbiome mediated DCA production, which up-regulated colonic TGR5 expression and GLP secretion, and improved glucose, lipid and energy metabolism in db/db mice.
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http://dx.doi.org/10.1016/j.biopha.2020.110953DOI Listing
December 2020

AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.

J Cell Sci 2020 12 7;133(23). Epub 2020 Dec 7.

Department of Animal Sciences, Chungbuk National University, Chungbuk, Cheongju 361-763, Republic of Korea

Activator of G-protein signaling 3 (AGS3, also known as GPSM1) regulates the trans-Golgi network. The AGS3 GoLoco motif binds to Gαi and thereby regulates the transport of proteins to the plasma membrane. Compaction of early embryos is based on the accumulation of E-cadherin (Cdh1) at cell-contacted membranes. However, how AGS3 regulates the transport of Cdh1 to the plasma membrane remains undetermined. To investigate this, AGS3 was knocked out using the Cas9-sgRNA system. Both trans-Golgi network protein 46 (TGN46, also known as TGOLN2) and transmembrane p24-trafficking protein 7 (TMED7) were tracked in early mouse embryos by tagging these proteins with a fluorescent protein label. We observed that the majority of the -edited embryos were developmentally arrested and were fragmented after the four-cell stage, exhibiting decreased accumulation of Cdh1 at the membrane. The trans-Golgi network and TMED7-positive vesicles were also dispersed and were not polarized near the membrane. Additionally, increased Gαi1 (encoded by ) expression could rescue -overexpressed embryos. In conclusion, AGS3 reinforces the dynamics of the trans-Golgi network and the transport of TMED7-positive cargo containing Cdh1 to the cell-contact surface during early mouse embryo development.
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http://dx.doi.org/10.1242/jcs.243238DOI Listing
December 2020

ZIF-8 derived Fe‒N coordination moieties anchored carbon nanocubes for efficient peroxymonosulfate activation via non-radical pathways: Role of FeN sites.

J Hazard Mater 2021 Mar 14;405:124199. Epub 2020 Oct 14.

Department of Environmental Science, Zhejiang University, Hangzhou, Zhejiang 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Hangzhou, Zhejiang 310058, China. Electronic address:

Developing high-efficient hybrids carbon catalysts for PMS-based advanced oxidation process (AOPs) are crucial in the field of environmental remediation. In this work, novel carbon nanocubes (xFe‒N‒C) with three-dimensional porous structure and abundant well-dispersed FeN sites were obtained via a skillful cage-encapsulated-precursor pyrolysis strategy. The as-synthesized xFe‒N‒C exhibited superb activity for phenol degradation by activating peroxymonosulfate (PMS). Besides, the catalytic system not only possessed good recycling performance, wide pH adaptation and relatively low activation energy, but also had high resistance to environmental interference. Singlet oxygen (O) dominated non-radical process was responsible for phenol degradation rather than traditional radical pathways. Impressively, the doping level of Fe could regulate FeN contents in catalysts, and the catalytic activity of xFe‒N‒C was greatly enhanced with increasing FeN contents. Based on density functional theory calculations (DFT), the introduction of FeN sites regulated the electronic structure of catalysts. Such electron-deficient Fe center acted as electron acceptor to receive electrons transmitted by the adsorbed PMS, thus generating highly reactive O for rapid phenol oxidation. This work provides a new insight into the innovation in transition metal-nitrogen hybrid carbon catalysts and highlights the pivotal roles of FeN sites in O generation during PMS activation process.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124199DOI Listing
March 2021

Coronavirus: A possible cause of reduced male fertility.

Andrology 2021 01 11;9(1):80-87. Epub 2020 Oct 11.

Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Changsha, China.

In lately December 2019, a novel coronavirus (SARS-CoV-2) outbreak occurred in Wuhan, PR China. It is a high contagious virus that has threatened human health worldwide. SARS-CoV-2 infection, termed COVID-19, causes rapidly developing lung lesions that can lead to multiple organ failure in a short period. Whenever a novel virus emerges, reproductive risk assessments should be performed after infection. In this review, we show that male fertility might be damaged by coronavirus associated with (i) direct cytopathic effects derived from viral replication and viral dissemination in the testis; and (ii) indirect damage to male fertility derived from immunopathology. In this review, we briefly describe the impaired fertility of humans and animals infected with coronaviruses to deduce the impact of the new coronavirus on male fertility. Together with information related to other coronaviruses, we extrapolate this knowledge to the new coronavirus SARS-CoV-2, which may have a significant impact on our understanding of the pathophysiology of this new virus.
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http://dx.doi.org/10.1111/andr.12907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537192PMC
January 2021

Experimental study on the permeability of Pb-contaminated silt solidified by CFG.

Environ Technol 2020 Oct 2:1-13. Epub 2020 Oct 2.

School of Civil Engineering and Architecture, Anhui University of Science and Technology, Huainan, People's Republic of China.

The permeability of soil is a fundamental parameter in hydrological and geotechnical studies, nevertheless, the investigations on the silt especially the heavy mental contaminated silt have rarely been reported. The research introduces the effect of the different factors, including the osmotic pressure, curing time, the content of binders and the concentration of lead, on the permeability and microscopic properties of lead-contaminated silt, which is solidified by a novel curing agent, formed by mixing cement, fly ash and desulphurization gypsum (CFG). According to the tests of the permeability and scanning electron microscope (SEM), the permeability of samples is investigated under different influencing factors. The results demonstrate that the permeability coefficient of the contaminated silt increases with the increasing osmotic pressure and lead ion concentration, while decreases with the increasing CFG content and the curing time, additonally, there are interactions among various factors, which jointly affect the permeability of samples. Moreover, the statistical analysis shows that osmotic pressure has the most significant effect on permeability among various factors. Besides, the influence of lead ion concentration, osmotic pressure and CFG content on the permeability coefficient at the curing time of 14d is more significant than that of 28d, and the influence order of factors was slightly different at different curing time. Furthermore, the SEM test proves the conclusion of the statistical analysis, which also explains the common reasons for the enhancement of the impermeability and unconfined compressive strength (UCS) of the lead-contaminated silt with the increasing curing time.
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http://dx.doi.org/10.1080/09593330.2020.1825532DOI Listing
October 2020

Accuracy of self-reported BMI using objective measurement in high school students.

J Nutr Sci 2020 12;9:e35. Epub 2020 Aug 12.

Department of Business Analytics and Statistics, University of Tennessee, 916 Volunteer Blvd., Knoxville, TN 37996-0532, USA.

Self-reported measures for body mass index (BMI) are considered a limitation in research design, especially when they are a primary outcome. Studies have found some populations to be quite accurate when self-reporting BMI; however, there is mixed research on the accuracy of self-reported measurements in adolescents. The aim of this study is to examine the accuracy of self-reported BMI by comparing it with measured BMI in a sample of U.S. adolescents and to understand gender differences. This cross-sectional study collected self-reported height and weight measurements of students from five high schools in four states (Tennessee, South Dakota, Kansas and Florida). Trained researchers took height and weight of students for an objective measurement. BMI was calculated from both sources and categorized (underweight, normal, overweight and obese) using the Centers for Disease Control and Prevention's BMI-for-age percentiles. Participants ( 425; 51⋅0 % female) had a mean age of 16⋅3 years old, and the majority were White (47⋅5 %). Limits of agreement (LOA) analysis revealed that BMI and weight were underreported, and height was overreported in the overall sample, in females, and in males. LOA analysis was fair for BMI in all three groups. Overall agreement in BMI categorisation was considered substantial ( 0⋅71, < 0⋅001). As BMI increased, more height and weight inaccuracies led to decreased accuracy in BMI categorisation, and the specificity of obese participants was low (50⋅0 %). This study's findings suggest that using self-reported values to categorize BMI is more accurate than using continuous BMI values when self-reported measures are used in health-related interventions.
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http://dx.doi.org/10.1017/jns.2020.28DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443770PMC
August 2021

Acylation modification of konjac glucomannan and its adsorption of Fe (Ⅲ) ion.

Carbohydr Res 2020 Nov 14;497:108133. Epub 2020 Aug 14.

College of Chemistry and Chemical Engineering, Neijiang Normal University, Neijiang, 641112, China. Electronic address:

A biodegradable adsorbent, modified konjac glucomannan (MKGM), was prepared by konjac glucomannan (KGM) acylated with phthalic anhydride catalyzed using concentrated sulfuric acid. The modified conditions such as reaction temperature, mass ratio of phthalic anhydride to KGM, catalyst dosage and reaction time were investigated, respectively. MKGM exhibited preferable adsorption performance for the removal of Fe (Ⅲ) ion. The adsorption behavior was discussed using the Langmuir and Freundlich isotherm models. The results showed that the Freundlich linear model was suitable for describing the adsorption process of Fe (Ⅲ). The maximum adsorption capacity of MKGM for Fe (Ⅲ) ion was 31.87 mg g at 298 K. The kinetics studies suggested that adsorption process followed the pseudo-second-order model and the adsorption process was mainly controlled by both surface reactivity and intra-particle diffusion. Together with the evaluation of the thermodynamic parameters such as Gibbs free energy, enthalpy and entropy changes, the results indicated that the adsorption process of Fe (Ⅲ) was endothermic, feasible, and spontaneous in nature. Hence, as a bioadsorbent, the MKGM has a promising potential for the removal of Fe (Ⅲ) ion from aqueous solutions.
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http://dx.doi.org/10.1016/j.carres.2020.108133DOI Listing
November 2020

Development and Validation of the Short Healthy Eating Index Survey with a College Population to Assess Dietary Quality and Intake.

Nutrients 2020 Aug 27;12(9). Epub 2020 Aug 27.

Department of Family, Youth, and Community Sciences, University of Florida, Gainesville, FL 32611, USA.

Because diet quality (DQ) is associated with risk of chronic disease and is a common construct assessed in health-related research, validated tools to assess DQ are needed that have low respondent and researcher burden. Thus, content experts develop the Short Healthy Eating Index (sHEI) tool and an associated scoring system. The sHEI scoring system was then refined using a classification and regression tree (CRT) algorithm methodology with an iterative feedback process with expert review and input. The sHEI scoring system was then validated using a concurrent criterion validation process that included the sHEI DQ scores (calculated from responses from 50 participants) being compared to the participants' Healthy Eating Index scores derived from 24 h recalls. The total HEI score from the CRT algorithm highly correlated with the 24 h recall HEI score (0.79). For individual food group items, the correlation between the CRT algorithm scoring and the 24 h recall data scoring ranged from 0.44 for refined grains to 0.64 for whole fruits. The sHEI appears to be a valid tool for estimating overall dietary quality and individual items (with correlations > 0.49) for fruits, vegetables, dairy, added sugar, sugar from sugar-sweetened beverages, and calcium.
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http://dx.doi.org/10.3390/nu12092611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551037PMC
August 2020
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