Publications by authors named "Wenjun Zhao"

102 Publications

Description of sp. nov., a phytopathogenic actinobacterium isolated from barley seeds, causing leaf brown spot and decline.

Int J Syst Evol Microbiol 2021 May;71(5)

Canadian Food Inspection Agency (CFIA), Charlottetown Laboratory, Charlottetown, PE C1A 5T1, Canada.

is a Gram-stain-positive bacterium with eight subspecies, five of which have been redefined as different species on the basis of their genome sequence data. On the basis of the results of phylogenetic analysis of gene sequences, strains of members of the genus isolated from barley have been grouped in a separate clade from other species and subspecies of the genus . In this study, the biochemical, physiological, fatty acids and genetic characteristics of strains DM1 and DM3, which represented the barley isolates, were examined. On the basis of results from multi-locus sequence typing and other biochemical and physiological features, including colony colour, carbon source utilisation and enzyme activities, DM1 and DM3 are categorically differentiated from the aforementioned eight species and subspecies of the genus . Moreover, the results of genomic analysis reveal that the DNA G+C contents of DM1 and DM3 are 73.7 and 73.5 %, respectively, and the average nucleotide identity (ANI) values between DM1 and DM3 and other species and subspecies range from 90.4 to 92.0 %. The ANI value between DM1 and DM3 is 98.0 %. These results indicate that DM1 and DM3 are distinct from other known species and subspecies of the genus . Therefore, we propose a novel species, , with DM1 (=CFCC 16553 =LMG 31970) as the type strain.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004786DOI Listing
May 2021

Comparison of ELF-EMFs stimulation with current stimulation on the regulation of LTP of SC-CA1 synapses in young rat hippocampus.

Int J Radiat Biol 2021 May 27:1-10. Epub 2021 May 27.

Shanghai Key Laboratory of Multidimensional Information Processing, East China Normal University, Shanghai, China.

Background: Long-term potentiation (LTP) is an important functional indicator for synaptic plasticity. Extremely low frequency electromagnetic fields (ELF-EMFs) are a physical means to regulate LTP, which induce induced currents. It is unknown whether induced current is the key factor when LTP is regulated by ELF-EMFs.New Method: A method is proposed for calculating the current value induced by ELF-EMFs. Then, a comparison of ELF-EMFs with current on the regulation of theta-burst or high-frequency stimulation (TBS/HFS)-LTP was performed.

Results: The LTP after ELF-EMFs and μA current regulation was significantly reduced. The regulatory effect of 0.1 μA current on LTP was similar with 100 Hz/2 mT ELF-EMFs, while 0.2 μA had a stronger regulatory effect than 200 Hz/2 mT on HFS-LTP.Comparison with Existing Methods: Most of the existing methods were used to calculate the induced current in human models, while we present a more accurate model for calculating the induced current induced by ELF-EMFs in the rat brain slices.

Conclusions: This work indicated that μA current and ELF-EMFs stimulation reduced LTP. Also, we demonstrated that the regulatory effect of ELF-EMFs on LTP is not entirely deriving from the induced current, since its magnetic mechanism might have played a certain role.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09553002.2021.1928781DOI Listing
May 2021

Seeded Growth of [email protected] Core-Shell Mesoporous Nanospheres and Their Photocatalytic Properties.

Front Chem 2021 23;9:671220. Epub 2021 Apr 23.

Department of Chemistry and Chemical Engineering, Jining University, Qufu, China.

We report a facile synthesis of [email protected] core-shell mesoporous nanospheres with tunable size in the aqueous phase seeded growth. The success of the current work relies on the use of a halide-free copper (Cu) precursor and n-oleyl-1,3-propanediamine as a capping agent to facilitate the formation of a copperish oxide shell with a mesoporous structure and the presence of mixed oxidation states of Cu. By varying the amount of spherical Au seeds while keeping other parameters unchanged, their diameters could be readily tuned without noticeable change in morphology. As compared with commercial CuO, the as-prepared [email protected] core-shell mesoporous nanospheres exhibit the higher adsorption ability, enhanced activity, and excellent stability toward photocatalytic degradation of methyl orange (MO) under visible light irradiation, indicating their potential applications in water treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2021.671220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103172PMC
April 2021

One-Stage Anchor-Free 3D Vehicle Detection from LiDAR Sensors.

Sensors (Basel) 2021 Apr 9;21(8). Epub 2021 Apr 9.

Beijing Laboratory of Intelligent Information Technology, School of Computer Science, Beijing Institute of Technology, Beijing 100081, China.

Recent one-stage 3D detection methods generate anchor boxes with various sizes and orientations in the ground plane, then determine whether these anchor boxes contain any region of interest and adjust the edges of them for accurate object bounding boxes. The anchor-based algorithm calculates the classification and regression label for each anchor box during the training process, which is inefficient and complicated. We propose a one-stage, anchor-free 3D vehicle detection algorithm based on LiDAR point clouds. The object position is encoded as a set of keypoints in the bird's-eye view (BEV) of point clouds. We apply the voxel/pillar feature extractor and convolutional blocks to map an unstructured point cloud to a single-channel 2D heatmap. The vehicle's Z-axis position, dimension, and orientation angle are regressed as additional attributes of the keypoints. Our method combines SmoothL1 loss and IoU (Intersection over Union) loss, and we apply (cosθ,sinθ) as angle regression labels, which achieve high average orientation similarity (AOS) without any direction classification tricks. During the target assignment and bounding box decoding process, our framework completely avoids any calculations related to anchor boxes. Our framework is end-to-end training and stands at the same performance level as the other one-stage anchor-based detectors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21082651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069010PMC
April 2021

[A Meta-analysis of risk factors related to the lymph nodes posterior to the right recurrent laryngeal nerve in thyroid micropapillary carcinoma].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Mar;35(3):245-251

Department of Thyroid Surgery,the First Hospital of Shanxi Medical University.

To evaluate the risk factors for metastasis of the lymph nodes posterior to the right recurrent laryngeal nerve in thyroid micropapillary carcinoma(PTMC) by Meta-analysis. We searched domestic and foreign databases for relevant studies published up to June, 2020. Two independent reⅥewers performed literature screening and data extraction according to the inclusion and exclusion criteria. Then, Meta-analysis was performed using Revman 5.3 software. A total of 5 articles were included, with a total of 1884 subjects.The results of the study showed that the rate of lymph node metastasis in PTMC Ⅵb area was 10.8% (203/1884). The risk factors for metastasis included patients' age <45 years old, male, right lobe tumor Diameter ≥0.5 cm, invading the envelope, Ⅵa lymph node metastasis. Ⅵb lymph node dissection is recommended when there exists age<45 years, male sex, right lobe tumor≥0.5 cm, capsular invasion or Ⅵa lymph node metastasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13201/j.issn.2096-7993.2021.03.012DOI Listing
March 2021

Effect of neuromuscular electrical stimulation associated with swallowing-related muscle training for post-stroke dysphagia: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Mar;100(11):e25108

Chengde Central Hospital, Chengde, Hebei Province, China.

Background: Swallowing dysfunction is a common dysfunction after stroke, and its incidence exceeds 50%. Aspiration pneumonia and malnutrition induced by dysphagia not only cause psychological shock to patients after stroke, but also burden the medical payment. Neuromuscular electrical stimulation, which stimulates the cortex and cortical bulb pathways to improve swallowing function, has been one of the emerging treatments for the post-stroke deglutition disorder. These therapy operators require the proficiency in professional knowledge, limiting clinical large sample studies, so there is an absence of evidence-based medicine. The research is to evaluate the effectiveness of neuromuscular electrical stimulations combined with swallowing-related muscle training to treat swallowing dysfunction after stroke.

Methods: Computer retrieval performed in the 9 databases, including PubMed, Embase, Web of science, Cochrane Library, ClinicalTrials, China Biomedical Literature Database (CBM), China Knowledge Network Database (CNKI), Wanfang Database (WanFang), and China VIP Database (VIP). Taking the published literature from the establishment of the database until December 20, 2020. Literature searching is related to neuromuscular electrical stimulation randomized controlled trials on the effect of swallowing in stroke. In addition, we will do the manual search in Baidu Academic and Google Academic database as a supplementary search. The correlative randomized controlled clinical studies retrieval time range from the establishment of the database to December 20, 2020. Two investigators will screen the literature according to the inclusion and exclusion criteria independently, during that period they will evaluate the quality of the included studies and extract data from studies. The extracted data are dichotomous data will be represented by relative risk, continuous data will be represented by mean difference or standard mean deviation. If there exists heterogeneity and the final data summary analysis select random effect model. On the contrary, the fixed effect model is selected. Then, RevMan5.3 software was used when analyzing included literature. Meanwhile, the analysis results were illustrated by drawing.

Results: This review will summarize available trials aimed at providing a comprehensive estimation of effectiveness of neuromuscular electrical stimulation associated with swallowing muscle training for post-stroke dysphagia.

Conclusion: This review based on a comprehensive analysis of currently published randomized controlled trials on post-stroke dysphagia, that provide reliable evidence-based medicine evidence for the efficacy of neuromuscular electrical stimulation associated with swallowing rehabilitation training.

Registration Number: INPLASY202110009.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982218PMC
March 2021

Treatment of common iliac vein aneurysms: case report and literature review.

Ann Transl Med 2021 Feb;9(4):360

Taizhou Hospital of Zhejiang Province, Affiliated to Wenzhou Medical University, Linhai, China.

In this study we report on a patient with a left common iliac vein aneurysm; a condition rarely seen in vascular surgery. A 49-year-old man with no history of trauma or surgery underwent computed tomography (CT) for the evaluation of lumbago. A subsequent 64-slice CT angiogram revealed a left common iliac vein aneurysm. Surgery was performed due to the possibility of rupture. The aneurysm was successfully treated with clipping and sutures and a pre-discharge CT showed the aneurysm had shrunk. We combine our experience treating this patient with a review of the characteristics of other reported cases and methods used to treat iliac vein aneurysms. A definitive diagnosis is recommended in patients in whom an iliac vein aneurysm is suspected, with a CT angiogram being a dependable diagnostic method. There is still no unified standard for the treatment of aneurysms. Conservative treatment can be chosen for some small aneurysms in early stage, but close follow-up is needed. Surgical treatment is still recommended for some large aneurysms. Because we know that if an aneurysm ruptures, it can be life-threatening. If appropriate artificial blood vessels or coated stents are available, aneurysms resection combined with artificial blood vessel reconstruction or directly coated stents are good choices for aneurysms isolation. For some very large aneurysms, I personally think that lateral wall resection and suture of our aneurysms is also a method. Early follow-up results also proved that the method was effective. Surgery should be performed as early as possible. Our method of surgery may be used as a selective surgical method if the aneurysm is large or saccular in morphology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-8189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944257PMC
February 2021

Deoxycytidine Kinase (DCK) Mutations in Human Acute Myeloid Leukemia Resistant to Cytarabine.

Acta Haematol 2021 Feb 24:1-8. Epub 2021 Feb 24.

Translational Medical Research Center, The Affiliated Wuxi No. 2 Peoples Hospital of Nanjing Medical University, Wuxi, China,

Resistance to cytarabine is an important cause of therapy failure in persons with acute myeloid leukemia (AML). Deoxycytidine kinase, encoded by DCK, catalyzes phosphorylation of cytarabine to cytarabine monophosphate, a necessary step for eventual incorporation of cytarabine triphosphate into DNA and for clinical efficacy. Whether DCK mutations make AML cells resistant to cytarabine is controversial. We studied DCK mutations and messenger RNA (mRNA) concentrations in leukemia cells from 10 subjects with AML who received cytarabine-based therapy and relapsed and in 2 artificially induced cytarabine-resistant AML cell lines. DCK mutations were detected in 4 subjects with AML relapsing after achieving a complete remission and receiving high-dose cytarabine postremission therapy. Most mutations were in exons 4-6 and were not present before therapy. DCK was also mutated in cytarabine-resistant but not parental AML cell lines. DCK mRNA concentrations were significantly decreased in cytarabine-resistant K562 and SHI-1 cells compared with cytarabine-sensitive parental cells. Mutation frequency of DCK and mRNA concentration did not correlate with the extent of cytarabine resistance indicating other factors operate. Overexpression of wild-type DCK restored cytarabine sensitivity to previously resistant leukemia cell lines. Our data contribute to the understanding of cytarabine resistance in persons with AML.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000513696DOI Listing
February 2021

Exploring the N Adsorption and Activation Mechanisms over the 2H/1T Mixed-Phase Ultrathin MoWS Nanosheets for Boosting N Photosynthesis.

ACS Appl Mater Interfaces 2021 Feb 8;13(6):7127-7134. Epub 2021 Feb 8.

College of Resource and Environmental Engineering, Guizhou University, Guiyang 550025, China.

Solar-driven conversion of nitrogen (N) to ammonia (NH) is highly appealing, yet in its infancy, the low photocatalytic efficiency and unclear adsorption and activation mechanisms of N are still issues to be addressed. In this study, ultrathin alloyed MoWS nanosheets with tunable hexagonal (2H)/trigonal (1T) phase ratios were proposed to boost photoreduction N efficiency, while the mechanisms of N adsorption and activation were explored simultaneously. The alloyed MoWS nanosheets for the 1T phase concentration of 33.6% and Mo/W = 0.68:0.32 were proven to reach about 111 μmol g h under visible light, which is 3.7 (or 3)-fold higher than that of pristine MoS (or WS). With the aid of density functional theory calculations and N adsorption X-ray absorption near-edge fine structure techniques, the adsorption and activation behaviors of N over the interface of MoWS nanosheets were investigated during the N reduction process. The results show that the W doping causes a higher electron density state in W 5d orbitals, which can further polarize the adsorbed N molecules for adsorption and activation. This work provides a new insight into the adsorption and activation mechanisms for the NH synthesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c19282DOI Listing
February 2021

FOXA3 induction under endoplasmic reticulum stress contributes to non-alcoholic fatty liver disease.

J Hepatol 2021 Feb 4. Epub 2021 Feb 4.

The Key Laboratory of Metabolism and Molecular Medicine of the Ministry of Education, Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai, China. Electronic address:

Background & Aims: Chronic endoplasmic reticulum (ER) stress in the liver has been shown to play a causative role in non-alcoholic fatty liver disease (NAFLD) progression, yet the underlying molecular mechanisms remain to be elucidated. Forkhead box A3 (FOXA3), a member of the FOX family, plays critical roles in metabolic homeostasis, although its possible functions in ER stress and fatty liver progression are unknown.

Methods: Adenoviral delivery, siRNA delivery, and genetic knockout mice were used to crease FOXA3 gain- or loss-of-function models. Tunicamycin (TM) and a high-fat diet (HFD) were used to induce acute or chronic ER stress in mice. ChIP-seq, luciferase assay, and adenoviral-mediated downstream gene manipulations were performed to reveal the transcriptional axis involved. Key axis protein levels in livers from healthy donors and patients with NAFLD were assessed via immunohistochemical staining.

Results: FOXA3 transcription is specifically induced by XBP1s upon ER stress. FOXA3 exacerbates the excessive lipid accumulation caused by the acute ER-inducer TM, whereas FOXA3 deficiency in hepatocytes and mice alleviates it. Importantly, FOXA3 deficiency in mice reduced diet-induced chronic ER stress, fatty liver, and insulin resistance. In addition, FOXA3 suppression via siRNA or adeno-associated virus delivery ameliorated the fatty liver phenotype in HFD-fed and db/db mice. Mechanistically, ChIP-Seq analysis revealed that FOXA3 directly regulates Period1 (Per1) transcription, which in turn promotes the expression of lipogenic genes, including Srebp1c, thus enhancing lipid synthesis. Of pathophysiological significance, FOXA3, PER1, and SREBP1c levels were increased in livers of obese mice and patients with NAFLD.

Conclusion: The present study identified FOXA3 as the bridging molecule that links ER stress and NAFLD progression. Our results highlighted the role of the XBP1s-FOXA3-PER1/Srebp1c transcriptional axis in the development of NAFLD and identified FOXA3 as a potential therapeutic target for fatty liver disease.

Lay Summary: The molecular mechanisms linking endoplasmic reticulum stress to non-alcoholic fatty liver disease (NAFLD) progression remain undefined. Herein, via in vitro and in vivo analysis, we identified Forkhead box A3 (FOXA3) as a key bridging molecule. Of pathophysiological significance, FOXA3 protein levels were increased in livers of obese mice and patients with NAFLD, indicating that FOXA3 could be a potential therapeutic target in fatty liver disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhep.2021.01.042DOI Listing
February 2021

Acute acrylonitrile exposure inhibits endogenous HS biosynthesis in rat brain and liver: The role of CBS/3-MPST-HS pathway in its astrocytic toxicity.

Toxicology 2021 03 21;451:152685. Epub 2021 Jan 21.

Department of Preventive Medicine and Public Health Laboratory Science, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, China; Center for Experimental Research, Affiliated Kunshan Hospital to Jiangsu University School of Medicine, Kunshan, Suzhou, Jiangsu, 215132, China. Electronic address:

Hydrogen sulfide (HS) as the third gasotransmitter molecule serves various biological regulatory roles in health and disease. Acrylonitrile (AN) is a common occupational toxicant and environmental pollutant, causing brain and liver damage in mammals. The biotransformation of AN is dependent-upon reduced glutathione (GSH), cysteine and other sulfur-containing compounds. However, the effects of AN on the endogenous HS biosynthesis pathway have yet to be determined. Herein, we demonstrated that a single exposure to AN (at 25, 50, or 75 mg/kg for 1, 6 or 24 h) decreased the endogenous HS content and HS-producing capacity in a dose-dependent manner, both in the cerebral cortex and liver of rats in vivo. In addition, the inhibitory effects of AN (1, 2.5, 5, 10 mM for 12 h) on the HS content and/or the expression of HS-producing enzymes were also found both in primary rat astrocytes and rat liver cell line (BRL cells). Impairment in the HS biosynthesis pathway was also assessed in primary rat astrocytes treated with AN. It was found that inhibition of the cystathionine-β-synthase (CBS)/3-mercaptopyruvate sulfurtransferase (3-MPST)-HS pathway with the CBS inhibitor or 3-MPST-targeted siRNA significantly increased the AN-induced (5 mM for 12 h) cytotoxicity in astrocytes. In turn, CBS activation or 3-MPST overexpression as well as exogenous NaHS supplementation significantly attenuated AN-induced cytotoxicity. Taken together, endogenous HS biosynthesis pathway was disrupted in rats acutely exposed to AN, which contributes to acute AN neurotoxicity in primary rat astrocytes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tox.2021.152685DOI Listing
March 2021

Effects of exogenous nerve growth factor on the expression of BMP-9 and VEGF in the healing of rabbit mandible fracture with local nerve injury.

J Orthop Surg Res 2021 Jan 21;16(1):74. Epub 2021 Jan 21.

Oral Maxillofacial Trauma and Orthognathic Surgery, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi, Guizhou, China.

Background: Mandibular fracture healing is a complex process involving nerves and growth factors. Nerve growth factor (NGF) not only facilitates the maintenance of sympathetic neurite growth but also stimulates other growth factors that can promote the essential osteogenesis and angiogenesis for fracture healing. Therefore, it is necessary to analyze the combined effects of NGF, bone morphogenic protein-9 (BMP-9), and vascular endothelial growth factor (VEGF) to accelerate the healing of mandible fractures.

Methods: The models of mandible fracture with local nerve injury established in 48 rabbits were randomly divided into nerve growth factor group (NGF group), gelatin sponge group (GS group), blank group, and intact group. The recovery of nerve reflex was assessed by observing the number of rabbits with lower lip responses to acupuncture. The fracture healing was observed with visual and CBCT, and then callus tissues from the mandibular fracture area were collected for hematoxylin and eosin (HE) staining observation, and the expression of BMP-9 and VEGF in callus at different stages was detected by quantitative real-time PCR (qRT-PCR).

Results: Needling reaction in the lower lip showed the number of animals with nerve reflex recovery was significantly higher in the NGF group than that in the GS and blank groups at the 2nd and 4th weeks after the operation. The combined results of macroscopic observation, CBCT examination, and histological analysis showed that a large number of osteoblasts and some vascular endothelial cells were found around the trabecular bone in the NGF group and the amount of callus formation and reconstruction was better than that in the GS group at the 2nd week after the operation. The qRT-PCR results indicated that the expression levels of BMP-9 and VEGF in the four groups reached the highest values at the 2nd week, while the expression levels of both in the NGF group were significantly higher than that in the GS group.

Conclusion: The exogenous NGF could accelerate the healing of mandible fractures. This work will provide a new foundation and theoretical basis for clarifying the mechanism of fracture healing, thereby promoting fracture healing and reducing the disability rate of patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-021-02220-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818757PMC
January 2021

The short-term outcome of residual thrombus of the lower extremity after pharmacomechanical catheter-directed thrombolysis for deep vein thrombosis.

Ann Transl Med 2020 Aug;8(16):1001

Department of Vascular Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Background: This study aimed to assess the short-term effect of residual thrombus of the lower extremity after pharmacomechanical catheter-directed thrombolysis (PCDT) on the incidence of post-thrombotic syndrome (PTS) and iliofemoral vein patency rate in patients who underwent PCDT.

Methods: There were 94 continuous patients with severe deep vein thrombosis (DVT) of the lower extremities admitted to our hospital between March 2016 and June 2018; 73 cases receiving PCDT and verified with iliac vein compression syndrome (IVCS) were assigned into two groups. Thirty-nine patients without thrombus of the popliteal and infrapopliteal veins were assigned to the proximal DVT group. The remaining 34 cases were placed in the extensive DVT group. The thrombus scores, including venous registry index (VRI) score, Marder score, and Society for Vascular Surgery (SVS) score before and after PCDT, the primary two years' cumulative rate of iliofemoral patency, and the rate of PTS, and complications were analyzed retrospectively.

Results: Ultimately, 27 patients in the proximal DVT group and 26 cases in the extensive DVT group completed the study. The two groups had no significant differences in terms of basic characteristics, complication and the rate of PTS (P>0.05). However, there were significant differences in terms of postoperative mean thrombus score and the mean degree of thrombosis removal score by Marder and SVS scores, residual thrombus by lower thrombosis classification (LET) and the two years' cumulative iliofemoral vein patency rate (P<0.05). And the logistic regression analysis demonstrated the residual thrombus of LET class II [odds ratio (OR), 4.619, 95% confidence interval (CI), 1.090-19.567, P=0.038] was an independent risk factor for iliofemoral vein occlusion.

Conclusions: The residual thrombus of LET class II is an independent risk factor for iliofemoral vein occlusion. It is very important to keep the patency of the popliteal vein when deciding to use a stent to maintain iliofemoral vein patency. Furthermore, the anterior tibial vein approach and crisscross technique may be two important alternative methods that can be used to address the thrombus of popliteal vein for patients with extensive DVT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-5459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475480PMC
August 2020

Genomic amplification of long noncoding RNA HOTAIRM1 drives anaplastic thyroid cancer progression via repressing miR-144 biogenesis.

RNA Biol 2021 04 20;18(4):547-562. Epub 2020 Sep 20.

Department of General Surgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.

Genomic aberrations are frequently found in anaplastic thyroid cancer (ATC). However, the functional genes in aberrantly genomic regions are largely unclear. In this study, we identified a long noncoding RNA (lncRNA) HOTAIRM1, whose encoding gene was amplified and expression was upregulated in ATC compared with papillary thyroid cancer and normal thyroid. Increased genomic copy number and expression of were both correlated with poor survival of ATC patients. Functional assays revealed that HOTAIRM1 promoted proliferation, inhibited apoptosis, and promoted migration and invasion of ATC cells , and promoted ATC tumour growth and metastasis . HOTAIRM1 was found to bind ILF3, repress the binding between ILF3 and precursor miR-144 (pre-miR-144), block the effects of ILF3 on stabilizing pre-miR-144, and therefore downregulate pre-miR-144. Intriguingly, HOTAIRM1 was also found to directly bind primary miR-144 (pri-miR-144), repress the binding between pri-miR-144 and DROSHA, block the processing of pri-miR-144 by DROSHA, and therefore upregulate pri-miR-144 and downregulate pre-miR-144. Thus, HOTAIRM1 remarkably downregulated pre-miR-144 and further downregulated miR-144. Knockdown of ILF3 and DROSHA abolished the effects of HOTAIRM1 on pre-miR-144 and miR-144. The expression of miR-144 was downregulated and reversely correlated with HOTAIRM1 in ATC. Via repressing miR-144 biogenesis, HOTAIRM1 upregulated MET and activated AKT signalling. miR-144 overexpression reversed the oncogenic roles of HOTAIRM1 in ATC. Altogether, these findings identified a genomic copy number amplified and highly expressed lncRNA HOTAIRM1, which exerted oncogenic roles via repressing miR-144 biogenesis in ATC. Our data suggested HOTAIRM1 as a potential prognostic biomarker and therapeutic target for ATC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/15476286.2020.1819670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971326PMC
April 2021

LINC00893 inhibits papillary thyroid cancer by suppressing AKT pathway via stabilizing PTEN.

Cancer Biomark 2021 ;30(3):277-286

Department of General Surgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.

Long non-coding RNAs (lncRNAs) are important to the occurrence and advancement of human cancers. We found through GEPIA that LINC00893 was lowly expressed in thyroid carcinoma (THCA) tissues, whereas the specific functions of LINC00893 has never been reported in PTC. In the current study, we confirmed that LINC00893 was expressed at a low level in PTC cells. Through gain-of-function assays, we determined that LINC00893 overexpression abrogated proliferation and migration abilities of PTC cells. Through signal transduction reporter array we found that LINC00893 potentially modulated the signals of phosphatase and tensin homolog (PTEN)/AKT pathway. In addition, overexpression of LINC00893 increased the expression of PTEN but reduced the levels of phosphorylated AKT in PTC. Additionally, mechanism assays unveiled that LINC00893 stabilized PTEN mRNA via recruiting Fused in sarcoma (FUS) protein. Finally, rescue assays demonstrated that LINC00893 hampered the proliferation and migration of PTC cells via PTEN/AKT pathway. Together, our study first clarified that LINC00893 functions as a tumor suppressor in PTC by blocking AKT pathway through PTEN upregulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3233/CBM-190543DOI Listing
January 2021

Prolonged infusion of linezolid is associated with improved pharmacokinetic/pharmacodynamic (PK/PD) profiles in patients with external ventricular drains.

Eur J Clin Pharmacol 2021 Jan 18;77(1):79-86. Epub 2020 Aug 18.

Department of Emergency Internal Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, China.

Objective: We previously investigated the pharmacokinetic and pharmacodynamic (PK/PD) parameters of routine linezolid infusions (1 h) in patients with external ventricular drains (EVD). The aim of the study was to determine whether extended linezolid infusions (200 mg/h for 3 h) were more efficacious than short linezolid infusions (600 mg/h for 1 h).

Methods: We collected cerebrospinal fluid (CSF) and plasma samples from 10 patients who received linezolid infusions after cerebral hemorrhage surgery with EVDs. Linezolid concentrations were measured by high-performance liquid chromatography (HPLC). A Monte Carlo simulation was used to measure the probability of target attainments (PTA) and the PK/PD indexes at four minimum inhibitory concentrations (MIC).

Results: When the same dose (600 mg) was given as an extended infusion (3 h), linezolid reached its maximum concentrations in the plasma and CSF at 3.00 h and 4.40 h, respectively. The mean penetration of linezolid in CSF was 41.31%. Using the parameter of AUC/MIC ≥ 100, the plasma PTA provided good coverage at > 90% when MIC was ≤ 1 μg/mL, while the values were 0 in CSF. Using the parameter %T (time) > MIC ≥ 85%, the PTA in both the plasma and CSF provided good coverage when MIC ≤ 2 μg/mL. Compared with routine infusions, prolonged infusion times (3 h) showed increased PTA of linezolid.

Conclusions: Prolonged infusion times increased the concentration of linezolid in the plasma, leading to improved therapeutic outcomes. However, this improvement did not exist in CSF. Lastly, the PK/PD indicator AUC/MIC ≥ 100 may be used to achieve improved outcomes in patients with critical infections.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00228-020-02978-xDOI Listing
January 2021

Circulating miRNA-21 as a diagnostic biomarker in elderly patients with type 2 cardiorenal syndrome.

Sci Rep 2020 03 17;10(1):4894. Epub 2020 Mar 17.

Department of Geriatric Cardiology, Hebei General Hospital, Shijiazhuang, Hebei, China.

Circulating miRNAs have attracted attention as serum biomarkers for several diseases. In this study, we aimed to evaluate the diagnostic value of circulating miRNA-21 (miR-21) as a novel biomarker for elderly patients with type 2 cardiorenal syndrome (CRS-2). A total of 157 elderly patients with chronic heart failure (CHF) were recruited for the study. According to an estimated glomerular filtration rate (eGFR) cut-off of 60 ml/min/1.73 m, 84 patients (53.5%) and 73 patients (46.5%) were assigned to the CRS group and the CHF group, respectively. Expression levels of serum miR-21 and biomarkers for CRS, such as kidney injury factor-1 (KIM-1), neutrophil gelatinase-related apolipoprotein (NGAL), cystatin C (Cys C), amino-terminal pro-B-type natriuretic peptide (NT-proBNP), N-acetyl-κ-D-glucosaminidase (NAG), and heart-type fatty acid-binding protein (H-FABP), were detected. Serum miR-21, KIM-1, NGAL, Cys C, NT-proBNP and H-FABP levels were significantly higher in the CRS group than in the CHF group (P < 0.01), whereas NAG expression was not significantly different between the two groups (P > 0.05). Cys C, H-FABP and eGFR correlated significantly with miR-21 expression, but correlations with miR-21 were not significant for NT-proBNP, NGAL, NAG and KIM-1. Moreover, multivariate logistic regression found that serum miR-21, increased serum Cys C, serum KIM-1, hyperlipidaemia and ejection fraction (EF) were independent influencing factors for CRS (P < 0.05). The AUC of miR-21 based on the receiver operating characteristic (ROC) curve was 0.749, with a sensitivity of 55.95% and a specificity of 84.93%. Furthermore, combining miR-21 with Cys C enhanced the AUC to 0.902, with a sensitivity of 88.1% and a specificity of 83.6% (P < 0.001). Our findings suggest that circulating miR-21 has medium diagnostic value in CRS-2. The combined assessment of miR-21 and Cys C has good clinical value in elderly patients with CRS-2.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-61836-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7078306PMC
March 2020

N -Methyladenosine Reader Protein YT521-B Homology Domain-Containing 2 Suppresses Liver Steatosis by Regulation of mRNA Stability of Lipogenic Genes.

Hepatology 2021 01 25;73(1):91-103. Epub 2020 Oct 25.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, People's Republic of China.

Background And Aims: Nonalcoholic fatty liver disease (NAFLD) is characterized by accumulation of excessive triglycerides (TGs) in hepatocytes. Obesity is a major risk factor for developing fatty liver, although the intracellular molecular basis remains largely unclear. N -methyladenosine (m A) RNA methylation is the most common internal modification in eukaryotic mRNA.

Approach And Results: In the present study, by m A sequencing and RNA sequencing, we found that both m A enrichment and mRNA expression of lipogenic genes were significantly increased in leptin-receptor-deficient db/db mice. Importantly, our results showed that YT521-B homology domain-containing 2 (Ythdc2), an m A reader, was markedly down-regulated in livers of obese mice and NAFLD patients. Suppression of Ythdc2 in livers of lean mice led to TG accumulation, whereas ectopic overexpression of Ythdc2 in livers of obese mice improved liver steatosis and insulin resistance. Mechanistically, we found that Ythdc2 could bind to mRNA of lipogenic genes, including sterol regulatory element-binding protein 1c, fatty acid synthase, stearoyl-CoA desaturase 1, and acetyl-CoA carboxylase 1, to decrease their mRNA stability and inhibit gene expression.

Conclusions: Our findings describe an important role of the m A reader, Ythdc2, for regulation of hepatic lipogenesis and TG homeostasis, which might provide a potential target for treating obesity-related NAFLD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/hep.31220DOI Listing
January 2021

Electrochemical removal of levofloxacin using conductive graphene/polyurethane particle electrodes in a three-dimensional reactor.

Environ Pollut 2020 May 31;260:114101. Epub 2020 Jan 31.

State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun, Jilin 130024, China. Electronic address:

The conductive polyurethane/polypyrrole/graphene (CPU/PPy/Gr) particle electrode was prepared by an in-situ oxidative polymerization method and used as particle electrodes to degrade levofloxacin (LEV) in a three-dimensional electrode reactor. The prepared CPU/PPy/Gr electrode was characterized systematically and the effects of initial pH, initial LEV concentration, aeration volume, voltage, and electrolyte concentration on the degradation efficiency were investigated. Results showed that more than 90% LEV was degraded and the energy consumption was 20.12 kWh/g LEV under conditions of pH 7, 6 V voltage, 2.0 L/min aeration volume, 20 mg/L initial LEV concentration, and 7 mM concentration of electrolyte (NaSO). A possible electrochemical oxidation pathway of LEV by the CPU/PPy/Gr electrode was proposed. In addition, the biotoxicity of LEV and its oxidation products was calculated using ECOSAR (Ecological Structure Activity Relationships) program in EPISuite. Toxicity evaluation using luminescent bacteria showed that the toxicities of some intermediates were higher than the parent compound. But the toxicity of degradation processes for LEV was effective decreasing. A possible reactive mechanism in the three-dimensional reactor was also recommended. In brief, the prepared CPU/PPy/Gr particle electrode constitutes an insight into the promising practical application in the wastewater treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2020.114101DOI Listing
May 2020

Clinical significance of vasculogenic mimicry, vascular endothelial cadherin and SOX4 in patients with esophageal squamous cell carcinoma.

Int J Clin Exp Pathol 2019 1;12(7):2462-2473. Epub 2019 Jul 1.

Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College Bengbu, Anhui Province, China.

Background: Vasculogenic mimicry (VM) plays an important role in invasion and metastasis of malignant tumor. High expression of vascular endothelial cadherin (VE-cahderin) in malignant tumor cells can promote the formation of VM. High expression of SOX4 (sex-determining region Y-related high-mobility group box 4) was found in esophageal squamous cell carcinoma (ESCC). It can promote the development of epithelial stromal transformation. Then, SOX4 can promote the formation of VM in ESCC.

Methods: Paraffin-embedded specimens of ESCC (with complete clinicopathological data) and normal esophageal mucosa adjacent to carcinoma (> 5 cm) were collected from January to December 2013. CD34/PAS was used to detect VM. The expression of VE-cadherin and SOX4 was used by immunohistochemistry. The patients were followed up in detail (survival time and survival status).

Results: SOX4, VM, and VE-cadherin were highly expressed in ESCC. Moreover, they were positively correlated. Survival analysis shows that the expressions of SOX4, VM, and VE-cadherin are associated with the patient's prognosis and can be independent prognostic factors for ESCC.

Conclusions: Studies suggests that SOX4, which is highly expressed in ESCC, is involved in the formation of VM. The combined detection of SOX4, VE-cadherin and VM expression can be used as biomarkers for invasion and metastasis of ESCC. These three markers can be used as powerful prognostic factors in patients with ESCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949576PMC
July 2019

Evaluation of the correlativity of gender determining region Y-box 4, N-cadherin, CD44 and E-cadherin expression in the prognosis of esophageal squamous cell carcinoma.

Int J Clin Exp Pathol 2019 1;12(5):1745-1756. Epub 2019 May 1.

Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College Bengbu 233003, Anhui Province, China.

Background: SOX4 is highly expressed in many different tumor types, and SOX4 has been reported in the literature to participate in tumor proliferation, damaging and movement by leading Epithelial-Mesenchymal Transition. Cancer vital cells and Epithelial-Mesenchymal Transition have been repeatedly confirmed to participate during the proliferation, damaging and movement of cancer. This research examined the association of the Epithelial-Mesenchymal Transition-related molecules E-cadherin, N-cadherin, CD44, and SOX4 in the ESCC and aimed for providing inspiration for clinical treatment as well as to indicate a new direction for detecting invasion and forecasting the prospect of affected role using ESCC.

Methods: Immunohistochemistry was utilized to observe the expression of the S0X4, N-cadherin, CD44 and E-cadherin proteins. Survival analysis of the positive and negative SOX4, E-cadherin, N-cadherin and CD44 protein expression groups was performed by the Kaplan-Meier approach.

Outcomes: A confirming relationship was observed among the expression of SOX4, N-cadherin or CD44 and tumor diameter, distant metastasis, deepness of damaging, lymph node metastasis, pTNM stage and histological grade (<0.05). Spearman correlativity calculation displayed that the expression of the SOX4 protein was obviously responded with the expression of the N-cadherin and CD44 proteins. Moreover, the expression of the N-cadherin and CD44 proteins was also positively correlated. The E-cadherin protein was negatively correlated with SOX4, N-cadherin and CD44 protein expression in ESCC. SOX4, N-cadherin, CD44, E-cadherin, age and distant metastasis were determined to be separate elements that influenced the prognosis of patients with ESCC.

Conclusions: We found that suppression of ESCC providers can suppress the growth of bad tumors and change therapeutic results for ESCC patient since CD44 supports the induction of Epithelial-Mesenchymal Transition in ESCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947138PMC
May 2019

Fowl adenoviruse-4 infection induces strong innate immune responses in chicken.

Comp Immunol Microbiol Infect Dis 2020 Feb 18;68:101404. Epub 2019 Dec 18.

College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China. Electronic address:

Fowl adenovirus (FAdV), as the causative agent of hepatitis-hydropericardium syndrome (HHS), poses a significant threat to the poultry industry in China in recent years. In this study, we investigated the immunopathogenesis of a FAdV-4 strain HN/151025 in 60-day-old chickens. The virus was highly virulent in chickens, with a broader tissue tropism in chickens, causing 60 % mortality. Postmortem findings of dead chickens showed mild HHS and liver degeneration and necrosis. Importantly, FAdV-4 infection induced significant upregulation of genes encoding most toll-like receptors, some cytokines (interleukin-1β, 2, 6, 8, and 18, and interferon-γ), most of avian β-defensins, myeloid differentiation primary response protein 88, p38 mitogen-activated protein kinases, and inducible nitric oxide synthase, in tissues of infected chicken, especially in spleen and bursa of Fabricius. There was also a significant positive correlation between FAdV-4 genome load and the mRNA expression levels of most of these factors in specific infected tissues. The results indicated the potential role of these proteins in host immune response against FAdV-4 infection. However, overexpression of these proteins might contribute to tissue damage of FAdV-4 infected chickens, and eventually lead to chicken death.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cimid.2019.101404DOI Listing
February 2020

Comparison of blast lung injury in infant and adult rabbits.

Pediatr Pulmonol 2020 02 3;55(2):474-483. Epub 2019 Dec 3.

Daping Hospital, Army Medical Center of the PLA, Research Institute of Surgery, Army Medical University, Chongqing, China.

Background: A great number of children suffer from blast lung injury (BLI) worldwide, but there is a little basic study on the topic.

Methods: Infant (4-week-old) and adult New Zealand rabbits were selected. Comparison of BLI characteristics: 16 infant rabbits were randomly divided into a 4.0 MPa group (n = 8, "IRG4.0MPa") and a 4.5 MPa group (n = 8, "IRG4.5MPa") and exposed to shock waves of those magnitudes. Eight adult rabbits were exposed to 4.0 MPa ("ARG4.0MPa"). The severity of BLI was compared among these three groups. Comparison of impact characteristics between infant and adult rabbits at the same scale of BLI: infant and adult rabbits were randomly divided into a control group (n = 8 per age group) and a blast injury group (n = 40 infants, "IRG4.0MPa"; n = 40 adults, "ARG4.5MPa"). The vital signs, physiological indicators, gross anatomy, and light microscope pathology were observed.

Results: The differences in lung injury severity and in abbreviated injury scale (AIS) scores between IRG4.5MPa (87.5%, AIS 4.13 ± 0.64) and ARG4.0MPa (75.5%, AIS 4.18 ± 0.75) were not statistically significant (P > .05); IRG4.0MPa (12.5%, AIS 3.38 ± 0.52) showed a lower proportion of lung injury and lower AIS scores than the others (P < .01). All rabbits showed languor immediately after the injury. The lungs showed tissue rupture, extensive patchy hemorrhage, edema, and inflammatory infiltration. The increase in lung water content was much higher in the IRG than in the ARG (P < .01).

Conclusion: Infant rabbits show better tolerance to lung injury caused by shock waves; infant rabbits show more disturbance on pathophysiological response than adult rabbits.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ppul.24593DOI Listing
February 2020

Reducing antigenicity of bovine whey proteins by Kluyveromyces marxianus fermentation combined with ultrasound treatment.

Food Chem 2020 May 16;311:125893. Epub 2019 Nov 16.

Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, PR China. Electronic address:

This work investigated the reduction of bovine whey proteins antigenicity by ultrasonic pretreatment and microbial fermentation. Firstly, bovine whey proteins was pretreated by ultrasonic techniques, and its secondary structure was detected by circular dichroism. The pretreated whey proteins was used as the fermentation substrate by Kluyveromyces marxianus for microbial transformation. The single factor design and Box-Behnken Design (BBD) were carried out with the aim to optimize culture temperature, initial pH, inoculum volume and rotation speed. After optimization process, culture temperature, initial pH, inoculum volume and rotation speed were determined. Under culture temperature 35 °C, pH 7.25, inoculum level 10% and shaking speed 150 rpm, the α-LA and β-LG antigenicity in bovine whey proteins were reduced by 29% and 53%, respectively. The findings showed that combined with microbial fermentation for hydrolysis of whey proteins, ultrasonic pretreatment can be used in order to produce hypoallergenic bovine whey proteins.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2019.125893DOI Listing
May 2020

The value of ultrasound for detecting hand fractures: A meta-analysis.

Medicine (Baltimore) 2019 Nov;98(44):e17823

Department of Health Management Center.

Backgrounds: Hand fractures are the second most common upper-extremity fractures. The standard X-ray has shortcomings, such as exposure to radiation. Ultrasound has been reported as an alternative method of detecting hand fractures. In this study, we used meta-analysis to assess the diagnostic value of ultrasound for hand fractures.

Methods: Web of Science, PubMed, Embase, and Cochrane Library databases were searched for relative citations up to June 2019. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), area under the summary receiver operating characteristic curve (AUC), and summary receiver operating characteristic (SROC) curve were estimated.

Results: Seven studies including 842 participants (845 examined hands) met our inclusion criteria. The pooled sensitivity, specificity, PLR, and NLR of ultrasound for detecting hand fractures were 91%, 96%, 20.66, and 0.09, respectively. The pooled DOR was 231.17, indicating a very powerful diagnostic ability of ultrasound. Meta-regression showed that there was no heterogeneity with respect to age, cut-off, the performer of the ultrasound, and the types of hand fractures.

Conclusions: Our results showed that ultrasound had an excellent diagnostic value for hand fractures. In clinic, we proposed using ultrasound as a first-line and radiation-free modality in detecting hand fractures, including phalanx and metacarpal fractures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000017823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946312PMC
November 2019

C/EBPδ-Slug-Lox1 axis promotes metastasis of lung adenocarcinoma via oxLDL uptake.

Oncogene 2020 01 27;39(4):833-848. Epub 2019 Sep 27.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, 500 Dong-Chuan Road, 200241, Shanghai, China.

Cancer cells undergo significant lipid metabolic reprogramming to ensure sufficient energy supply for survival and progression. However, how cancer cells integrate lipid metabolic signaling with cancer progression is not well understood. In the present study, we demonstrated that C/EBPδ, a critical lipid metabolic regulator, is a TGF-β1 downstream gene and promotes lung adenocarcinoma metastasis. Importantly, C/EBPδ caused significant oscillations in both lipid metabolic and epithelial to mesenchymal transition (EMT) gene networks. Mechanistically, we demonstrated that C/EBPδ recruited oncogene NCOA3 to transcriptionally activate Slug, a canonical EMT transcription factor, which in turn induced oxLDL receptor-1 (Lox1) expression and enhanced oxLDL uptake to promote cancer metastasis, which could be blocked with LOX1 neutralizing antibody. In summary, our results unveiled a previously unappreciated interplay between lipid metabolic and metastatic program, as well as the existence of a pivotal C/EBPδ-Slug-Lox1 transcription axis to promote oxLDL levels and cancer metastasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41388-019-1015-zDOI Listing
January 2020

Continuous flow electrosorption-microbial fuel cell system for efficient removal of oxytetracycline without external electrical supply.

Bioresour Technol 2019 Oct 3;290:121751. Epub 2019 Jul 3.

State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration, School of Environment, Northeast Normal University, Changchun 130117, China. Electronic address:

The removal of antibiotics from wastewater has attracted much attention. In this research, an intimately coupled autarkical electrosorption (ES)-microbial fuel cell (MFC) system was developed for real-time removal of the antibiotic oxytetracycline (OTC) from wastewater. The removal efficiency was founded to be up to 98.8% at an OTC concentration of 2 mg/L with 3 g/L sodium acetate (NaAC) as co-substrate and 3 MFCs as power supply. The removal efficiencies increased in the ES unit and decreased in the MFC unit with increasing treating time. The adsorption of OTC on activated carbon fibers (ACFs) in the ES unit proceeds via chemical adsorption resulting from electrostatic attraction and cation exchange. The OTC degradation pathways in the MFC unit were proposed by identifying the intermediates with HPLC-MS/MS. The ACFs in the ES unit were proven to be recyclable and the coupled ES-MFC system is applicable for the removal of antibiotics from wastewater.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.121751DOI Listing
October 2019

[One case of hepatosplenic artery aneurysm with hepato-splenomesenteric trunk and literature review].

Zhejiang Da Xue Xue Bao Yi Xue Ban 2018 Dec;47(6):601-605

Department of Vascular Surgery, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Taizhou 317000, Zhejiang Province, China.

An asymptomatic 71-year-old woman was admitted to the hospital due to aneurysm of visceral artery. CT angiography revealed that she possessed a hepatosplenic artery aneurysm with hepatosplenomesenteric trunk anomaly. The aneurysm was big with diameter about 28 mm, and is very adjacent to the superior mensenteric artery. The neck of the aneurysm is wide (the diameter of the neck was 5.5-6.0 mm) and short (length of the proximal landing zone was about 2.0 mm). The patient received endovascular reconstruction of the hepatosplenic artery and coil embolization of the aneurysm, and got satisfactory result.
View Article and Find Full Text PDF

Download full-text PDF

Source
December 2018

Silver nanoparticles inhibit beige fat function and promote adiposity.

Mol Metab 2019 04 24;22:1-11. Epub 2019 Jan 24.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China. Electronic address:

Objective: Obesity is a complex chronic disease of high prevalence worldwide. Multiple factors play integral roles in obesity development, with rising interest focusing on the contribution of environmental pollutants frequent in modern society. Silver nanoparticles (AgNPs) are widely used for bactericidal purpose in various applications in daily life. However, their potential toxicity and contribution to the obesity epidemic are not clear.

Methods: Beige adipocytes are newly discovered adipocytes characterized by high thermogenic and energy dissipating capacity upon activation and the "browning" process. In the present study, we assess the impact of AgNPs exposure on beige adipocytes differentiation and functionality both in vitro and in vivo. We also systematically investigate the influence of AgNPs on adiposity and metabolic performance in mice, as well as the possible underlying molecular mechanism.

Results: The results showed that, independent of particle size, AgNPs inhibit the adipogenic, mitochondrial, and thermogenic gene programs of beige adipocytes, thus suppressing their differentiation ability, mitochondrial activity, and thermogenic response. Importantly, exposure to AgNPs in mice suppresses browning gene programs in subcutaneous fat, leading to decreased energy expenditure and increased adiposity in mice. Mechanistically, we found that AgNPs increase reactive oxidative species (ROS) levels and specifically activate MAPK-ERK signaling in beige adipocytes. The negative impacts of AgNPs on beige adipocytes can be ameliorated by antioxidant or ERK inhibitor FR180204 treatment.

Conclusions: Taken together, these results revealed an unexpected role of AgNPs in promoting adiposity through the inhibition of beige adipocyte differentiation and functionality, possibly by disrupting ROS homeostasis and ERK phosphorylation. Future assessments on the health risk of AgNPs applications and their safe dosages are warranted.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.molmet.2019.01.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437600PMC
April 2019

Circular RNA EIF6 (Hsa_circ_0060060) sponges miR-144-3p to promote the cisplatin-resistance of human thyroid carcinoma cells by autophagy regulation.

Aging (Albany NY) 2018 12;10(12):3806-3820

Department of General Surgery, First Hospital of Shanxi Medical University, Taiyua, Shanxi 030001, China.

Anaplastic thyroid carcinoma (ATC) responds for the majority of death of thyroid carcinoma and often causes chemotherapy resistance. We investigated the influence of circEIF6 (Hsa_circ_0060060) on the cisplatin-sensitivity in papillary thyroid carcinoma (PTC) and ATC cells, and explored its regulation to downstream molecules miR-144-3p and Transforming Growth Factor α (TGF-α). Differentially expressed circRNAs in PTC were analyzed using the GSE93522 data downloaded. Expressions of circEIF6, miR-144-3p, TGF-α, autophagy-related proteins and apoptosis-related proteins were determined using qRT-PCR or western blot. RNA pull-down assay and dual luciferase report assay were applied to reveal the target relationships. Autophagy marker LC3 and cell proliferation marker ki67 were evaluated by immunofluorescence and immunohistochemistry. Cell viability was evaluated with MTT assay and cell apoptosis was assessed by flow cytometric analysis. CircEIF6, could promote autophagy induced by cisplatin, thus inhibiting cell apoptosis and enhancing the resistance of PTC and ATC cells to cisplatin. Has-miR-144-3p was the target of circEIF6 and was regulated by circEIF6. Besides, circEIF6 promoted autophagy by regulating miR-144-3p/TGF-α axis, enhancing the cisplatin-resistance in PTC and ATC cells. CircEIF6 promoted tumor growth by regulating miR-144-3p/TGF-α and circEIF6 knock-down enhanced cisplatin sensitivity . CircEIF6 could provide a target for therapy of cisplatin-resistance in thyroid carcinoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.101674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326687PMC
December 2018