Publications by authors named "Wenjun Li"

716 Publications

D. Don Polysaccharides Inhibit High Glucose-Induced Proliferation and Angiogenesis of Retinal Vascular Endothelial Cells.

Diabetes Metab Syndr Obes 2021 31;14:2431-2440. Epub 2021 May 31.

Department of Ophthalmology, Jingmen NO.2 People's Hospital, Jingmen, 448000, People's Republic of China.

Purpose: The traditional Chinese medicine D. Don () has been reported to exhibit anti-cancer and anti-inflammation activities. The ethanol extract of has been confirmed to attenuate diabetic retinopathy (DR). This study aimed to investigate the effects and underlying mechanisms of the polysaccharides isolated from (PSB) on the proliferation and angiogenesis of retinal vascular endothelial cells (RVECs) in DR.

Methods: Human RVECs (HRVECs) were cultured in normal glucose (NG, 5.5 mM), mannitol (MA, 30 mM), high glucose (HG, 30 mM) and HG plus 40 μg/mL PSB, respectively. Then, cell proliferation, migration and angiogenesis were evaluated. The cell proliferation was also estimated in the presence of SLIGKV, which was used to induce the phosphorylation of ERK (p-ERK).

Results: PSB reduced normal and HG-induced HRVECs cell viability in a concentration-dependent manner. The protein expression of proliferating cell nuclear antigen (PCNA) and proliferating antigen KI67 (Ki67), the migration rate and tube formation ability, which were increased by HG treatment, were significantly decreased by PSB. PSB also inhibited the phosphorylation of Raf, MEK and ERK in HG-stimulated HRVECs. Moreover, the application of SLIGKV recovered cell viability and the expression of p-ERK, PCNA and Ki67, in HG plus PSB-treated cells. Finally, the HG-enhanced expression of VE-cadherin, Frizzed, β-catenin, MMP-2 and MMP-9 was all reversed by PSB.

Conclusion: PSB could inhibit HG-induced HRVECs proliferation, migration and neovascularization, and these effects might work through blocking the activation of MEK/ERK pathway and VEGF/VE-cadherin axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/DMSO.S296164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180288PMC
May 2021

Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins.

J Food Sci 2021 May 30. Epub 2021 May 30.

Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, PR China.

Hydrolysates containing angiotensin I-converting enzyme (ACE)-inhibitory peptide were prepared from protein of Alaska pollack skins using alcalase and trypsin. The protein hydrolysate was separated by ultrafiltration, Sephadex G-25 gel filtration chromatography and reversed phase high-performance liquid chromatography (HPLC), from which a novel purified peptide was obtained. Both random coil structure and β-sheet in the purified peptide were revealed in Fourier transform infrared spectrum. The amino sequence of the purified peptide was identified as GPLGVP, VLYPVK, VFLENVLR, and FEEF by HPLC-Q-TOF-MS (HPLC-quadrupole time-of-flight mass spectrometry). The peptide GPLGVP whose molecular weight was 538.31 Da showed the highest ACE inhibitory activity (IC = 105.8 µM). The purified peptide featured a noncompetitive inhibition kinetic mechanism was shown in the Lineweaver-Burk plots and was susceptible to enzymes as indicated in the studies on stability of gastrointestinal proteases. Moreover, the peptide GPLGVP can combine ACE catalytic pocket through hydrogen bonds and other forces with high binding power as disclosed in molecular docking simulation, which provides the inhibitory effect of GPLGVP on ACE.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1750-3841.15754DOI Listing
May 2021

Innate immunity in lung transplantation.

J Heart Lung Transplant 2021 Mar 26. Epub 2021 Mar 26.

Department of Surgery, Washington University School of Medicine, Saint Louis, Missouri; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, Missouri. Electronic address:

Innate immune pathways early after pulmonary transplantation have been shown to cause primary graft dysfunction (PGD) and also predispose to late graft failure. Recent studies in animal models have elucidated critical mechanisms governing such innate immune responses. Here, we discuss pathways of inflammatory cell death, triggers for sterile and infectious inflammation, and signaling cascades that mediate lung injury early after transplantation. These studies highlight potential avenues for lung-specific therapies early following lung transplantation to dampen innate immune responses and improve outcomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.healun.2021.03.017DOI Listing
March 2021

AKR1C3 decreased CML sensitivity to Imatinib in bone marrow microenvironment via dysregulation of miR-379-5p.

Cell Signal 2021 Aug 11;84:110038. Epub 2021 May 11.

Department of Physiology, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, Jiangsu, China. Electronic address:

Background: Drug resistance is an important cause of death for most patients with chronic myeloid leukemia (CML). The bone marrow microenvironment is believed to be mainly responsible for resistance to BCR-ABL tyrosine kinase inhibitors. The mechanism involved, however, is still unclear.

Methods: Bioinformatic analysis from GEO database of AKR1C3 was utilized to identify the AKR1C3 expression in CML cells under bone marrow microenvironment. Western blot and qPCR were performed to detect the AKR1C3 expression in two CML cell lines K562 and KU812 cultured +/- bone microenvironment derived stromal cells. CCK-8, soft agar colony assay, and Annexin V/PI assay were performed to detect the sensitivity of CML cells (K562 and KU812) to Imatinib under a gain of or loss of function of AKR1C3 treatment. The CML murine model intravenous inoculated with K562-OE-vector and K562-OE-AKR1C3 cells were established to estimate the effect of AKR1C3 inhibitor Indomethacin on Imatinib resistance. The bioinformatic analysis of miRNA databases was used to predict the potential miRNAs targeting AKR1C3. And the luciferase assay was utilized to validate the target relationship between miR-379-5p and AKR1C3. And, the soft agar colony assay and Annexin V/PI were used to validate the effect of miR-379-5p in AKR1C3 induced Imatinib resistance.

Results: In present study, we investigated AKR1C3 was highly expressed in CML under bone marrow microenvironment. AKR1C3 decreased Imatinib activity in K562 and KU812 cells, while inhibition of AKR1C3 could enhance Imatinib sensitivity in vitro study. Furthermore, murine model results showed combination use of AKR1C3 inhibitor Indomethacin effectively prolong mice survival, indicating that AKR1C3 is a promising target to enhance Imatinib treatment. Mechanically, AKR1C3 was found to be suppressed by miR-379-5p, which was down-expression in bone marrow microenvironment. Besides, we found miR-379-5p could bind AKR1C3 3'UTR but not degrade its mRNA level. Further, gain of miR-379-5p rescued the imatinib resistance induced by AKR1C3 overexpression in CML cells.

Conclusions: Altogether, our study identifies a novel signaling regulation of miR-379-5p/AKR1C3/EKR axis in regulating IM resistance in CML cell, and provides a scientific base for exploring AKR1C3 as a biomarker in impeding IM resistance in CML.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cellsig.2021.110038DOI Listing
August 2021

Insights into the endophytic bacterial community comparison and their potential role in the dimorphic seeds of halophyte Suaeda glauca.

BMC Microbiol 2021 May 12;21(1):143. Epub 2021 May 12.

State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Science, Sun Yat-Sen University, Guangzhou, 510275, China.

Background: Seed dimorphism has been thought to be a bet-hedging strategy that helps plants survive in the disturbed environment and has been widely studied for its ecological adaptation mechanism. Many studies showed that seed-associated microorganisms play an important role in enhancing plant fitness, but information regarding endophytic bacteria associated with dimorphic seeds is limited. This study explores the influence of seed coat structure and seed phytochemical properties on the community composition and diversity of endophytic bacteria of dimorphic seeds of Suaeda glauca. In this study, we used 16S rRNA high-throughput gene sequencing method to compare the community composition and bacterial diversity between brown and black seeds of Suaeda glauca.

Results: A significant difference was observed in seed coat structure and phytochemical properties between brown and black seeds of S. glauca. Total 9 phyla, 13 classes, 31 orders, 53 families, 102 genera were identified in the dimorphic seeds. The dominant phyla were Proteobacteria, Firmicutes, and Actinobacteria. The results showed that seed dimorphism had little impact on the diversity and richness of endophytic bacterial communities but significantly differs in the relative abundance of the bacterial community between brown and black seeds. At the phylum level, Actinobacteria tend to be enriched significantly in brown seeds. At the genus level, Rhodococcus, Ralstonia, Pelomonas and Bradyrhizobium tend to be enriched significantly in brown seeds, while Marinilactibacillus was mainly found in black seeds. Besides, brown seeds harbored a large number of bacteria with plant-growth-promoting traits, whereas black seeds presented bacteria with enzyme activities (i.e., pectinase, cellulolytic and xylanolytic activities).

Conclusion: The endophytic bacterial community compositions were significantly different between dimorphic seeds of Suaeda glauca, and play an important role in the ecological adaptation of dimorphic seeds by performing different biological function roles. The endophytic bacterial communities of the dimorphic seeds may be influenced mainly by the seed coat structureand partly by the seed phytochemical characteristics. These findings provide valuable information for better understanding of the ecological adaptation strategy of dimorphic seeds in the disturbed environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12866-021-02206-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114534PMC
May 2021

Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals.

Nat Commun 2021 05 7;12(1):2563. Epub 2021 May 7.

National Centre for Gene Research, CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), Centre of Excellence for Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS), Shanghai, China.

Non-random gene organization in eukaryotes plays a significant role in genome evolution. Here, we investigate the origin of a biosynthetic gene cluster for production of defence compounds in oat-the avenacin cluster. We elucidate the structure and organisation of this 12-gene cluster, characterise the last two missing pathway steps, and reconstitute the entire pathway in tobacco by transient expression. We show that the cluster has formed de novo since the divergence of oats in a subtelomeric region of the genome that lacks homology with other grasses, and that gene order is approximately colinear with the biosynthetic pathway. We speculate that the positioning of the late pathway genes furthest away from the telomere may mitigate against a 'self-poisoning' scenario in which toxic intermediates accumulate as a result of telomeric gene deletions. Our investigations reveal a striking example of adaptive evolution underpinned by remarkable genome plasticity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-22920-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105312PMC
May 2021

Prevalence of Frailty and Associations with Oral Anticoagulant Prescribing in Atrial Fibrillation.

J Gen Intern Med 2021 May 4. Epub 2021 May 4.

Department of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, MA, USA.

Background: Frailty is often cited as a factor influencing oral anticoagulation (OAC) prescription in patients with non-valvular atrial fibrillation (NVAF). We sought to determine the prevalence of frailty and its association with OAC prescription in older veterans with NVAF.

Methods: We used ICD-9 codes in Veterans Affairs (VA) records and Medicare claims data to identify patients with NVAF and CHADSVASC ≥2 receiving care between February 2010 and September 2015. We examined rates of OAC prescription, further stratified by direct oral anticoagulant (DOAC) or vitamin K antagonist (VKA). Participants were characterized into 3 categories: non-frail, pre-frail, and frail based on a validated 30-item EHR-derived frailty index. We examined relations between frailty and OAC receipt; and frailty and type of OAC prescribed in regression models adjusted for factors related to OAC prescription.

Results: Of 308,664 veterans with NVAF and a CHADSVASC score ≥2, 121,839 (39%) were prescribed OAC (73% VKA). The mean age was 77.7 (9.6) years; CHADSVASC and ATRIA scores were 4.6 (1.6) and 5.0 (2.9) respectively. Approximately a third (38%) were frail, another third (32%) were pre-frail, and the remainder were not frail. Veterans prescribed OAC were younger, had higher bleeding risk, and were less likely to be frail than participants not receiving OAC (all p's<0.001). After adjustment for factors associated with OAC use, pre-frail (OR: 0.89, 95% CI: 0.87-0.91) and frail (OR: 0.66, 95% CI: 0.64-0.68) veterans were significantly less likely to be prescribed OAC than non-frail veterans. Of those prescribed OAC, pre-frail (OR:1.27, 95% CI: 1.22-1.31) and frail (OR: 1.75, 95% CI: 1.67-1.83) veterans were significantly more likely than non-frail veterans to be prescribed a DOAC than a VKA.

Conclusions: There are high rates of frailty among older veterans with NVAF. Frailty using an EHR-derived index is associated with decreased OAC prescription.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11606-021-06834-1DOI Listing
May 2021

Metagenome-assembled genomes infer potential microbial metabolism in alkaline sulphidic tailings.

Environ Microbiome 2021 Apr 29;16(1). Epub 2021 Apr 29.

Hebei Key Laboratory of Soil Ecology, Key Laboratory for Agricultural Water Resource, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, China.

Background: Mine tailings are hostile environment. It has been well documented that several microbes can inhabit such environment, and metagenomic reconstruction has successfully pinpointed their activities and community structure in acidic tailings environments. We still know little about the microbial metabolic capacities of alkaline sulphidic environment where microbial processes are critically important for the revegetation. Microbial communities therein may not only provide soil functions, but also ameliorate the environment stresses for plants' survival.

Results: In this study, we detected a considerable amount of viable bacterial and archaeal cells using fluorescent in situ hybridization in alkaline sulphidic tailings from Mt Isa, Queensland. By taking advantage of high-throughput sequencing and up-to-date metagenomic binning technology, we reconstructed the microbial community structure and potential coupled iron and nitrogen metabolism pathways in the tailings. Assembly of 10 metagenome-assembled genomes (MAGs), with 5 nearly complete, was achieved. From this, detailed insights into the community metabolic capabilities was derived. Dominant microbial species were seen to possess powerful resistance systems for osmotic, metal and oxidative stresses. Additionally, these community members had metabolic capabilities for sulphide oxidation, for causing increased salinity and metal release, and for leading to N depletion.

Conclusions: Here our results show that a considerable amount of microbial cells inhabit the mine tailings, who possess a variety of genes for stress response. Metabolic reconstruction infers that the microbial consortia may actively accelerate the sulphide weathering and N depletion therein.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40793-021-00380-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086273PMC
April 2021

Magnetically Recyclable Wool Keratin Modified Magnetite Powders for Efficient Removal of Cu Ions from Aqueous Solutions.

Nanomaterials (Basel) 2021 Apr 21;11(5). Epub 2021 Apr 21.

Research Centre for Functional Textile Materials, School of Textile Science and Engineering, Xi'an Polytechnic University, Xi'an, Shaanxi 710048, China.

The treatment of wastewater containing heavy metals and the utilization of wool waste are very important for the sustainable development of textile mills. In this study, the wool keratin modified magnetite (FeO) powders were fabricated by using wool waste via a co-precipitation technique for removal of Cu ions from aqueous solutions. The morphology, chemical compositions, crystal structure, microstructure, magnetism properties, organic content, and specific surface area of as-fabricated powders were systematically characterized by various techniques including field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), thermogravimetric (TG) analysis, and Brunauer-Emmett-Teller (BET) surface area analyzer. The effects of experimental parameters such as the volume of wool keratin hydrolysate, the dosage of powder, the initial Cu ion concentration, and the pH value of solution on the adsorption capacity of Cu ions by the powders were examined. The experimental results indicated that the Cu ion adsorption performance of the wool keratin modified FeO powders exhibited much better than that of the chitosan modified ones with a maximum Cu adsorption capacity of 27.4 mg/g under favorable conditions (0.05 g powders; 50 mL of 40 mg/L CuSO; pH 5; temperature 293 K). The high adsorption capacity towards Cu ions on the wool keratin modified FeO powders was primarily because of the strong surface complexation of -COOH and -NH functional groups of wool keratins with Cu ions. The Cu ion adsorption process on the wool keratin modified FeO powders followed the Temkin adsorption isotherm model and the intraparticle diffusion and pseudo-second-order adsorption kinetic models. After Cu ion removal, the wool keratin modified FeO powders were easily separated using a magnet from aqueous solution and efficiently regenerated using 0.5 M ethylene diamine tetraacetic acid (EDTA)-HSO eluting. The wool keratin modified FeO powders possessed good regenerative performance after five cycles. This study provided a feasible way to utilize waste wool textiles for preparing magnetic biomass-based adsorbents for the removal of heavy metal ions from aqueous solutions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano11051068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143369PMC
April 2021

Chromosome-scale assembly and analysis of biomass crop Miscanthus lutarioriparius genome.

Nat Commun 2021 04 28;12(1):2458. Epub 2021 Apr 28.

National Center for Gene Research, State Key Laboratory of Plant Molecular Genetics, CAS Center of Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200233, China.

Miscanthus, a rhizomatous perennial plant, has great potential for bioenergy production for its high biomass and stress tolerance. We report a chromosome-scale assembly of Miscanthus lutarioriparius genome by combining Oxford Nanopore sequencing and Hi-C technologies. The 2.07-Gb assembly covers 96.64% of the genome, with contig N50 of 1.71 Mb. The centromere and telomere sequences are assembled for all 19 chromosomes and chromosome 10, respectively. Allotetraploid origin of the M. lutarioriparius is confirmed using centromeric satellite repeats. The tetraploid genome structure and several chromosomal rearrangements relative to sorghum are clearly demonstrated. Tandem duplicate genes of M. lutarioriparius are functional enriched not only in terms related to stress response, but cell wall biosynthesis. Gene families related to disease resistance, cell wall biosynthesis and metal ion transport are greatly expanded and evolved. The expansion of these families may be an important genomic basis for the enhancement of remarkable traits of M. lutarioriparius.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-22738-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080599PMC
April 2021

A novel linker-immunodominant site (LIS) vaccine targeting the SARS-CoV-2 spike protein protects against severe COVID-19 in Syrian hamsters.

Emerg Microbes Infect 2021 Dec;10(1):874-884

CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, People's Republic of China.

The Coronavirus Disease 2019 (COVID-19) pandemic is unlikely to abate until sufficient herd immunity is built up by either natural infection or vaccination. We previously identified ten linear immunodominant sites on the SARS-CoV-2 spike protein of which four are located within the RBD. Therefore, we designed two linkerimmunodominant site (LIS) vaccine candidates which are composed of four immunodominant sites within the RBD (RBD-ID) or all the 10 immunodominant sites within the whole spike (S-ID). They were administered by subcutaneous injection and were tested for immunogenicity and protective efficacy in a hamster model for COVID-19. We showed that the S-ID vaccine induced significantly better neutralizing antibody response than RBD-ID and alum control. As expected, hamsters vaccinated by S-ID had significantly less body weight loss, lung viral load, and histopathological changes of pneumonia. The S-ID has the potential to be an effective vaccine for protection against COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/22221751.2021.1921621DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118541PMC
December 2021

A Framework for Automatic Burn Image Segmentation and Burn Depth Diagnosis Using Deep Learning.

Comput Math Methods Med 2021 7;2021:5514224. Epub 2021 Apr 7.

The People's Hospital of Jianggan District, Hangzhou, Zhejiang, China.

Burn is a common traumatic disease with high morbidity and mortality. The treatment of burns requires accurate and reliable diagnosis of burn wounds and burn depth, which can save lives in some cases. However, due to the complexity of burn wounds, the early diagnosis of burns lacks accuracy and difference. Therefore, we use deep learning technology to automate and standardize burn diagnosis to reduce human errors and improve burn diagnosis. First, the burn dataset with detailed burn area segmentation and burn depth labelling is created. Then, an end-to-end framework based on deep learning method for advanced burn area segmentation and burn depth diagnosis is proposed. The framework is firstly used to segment the burn area in the burn images. On this basis, the calculation of the percentage of the burn area in the total body surface area (TBSA) can be realized by extending the network output structure and the labels of the burn dataset. Then, the framework is used to segment multiple burn depth areas. Finally, the network achieves the best result with IOU of 0.8467 for the segmentation of burn and no burn area. And for multiple burn depth areas segmentation, the best average IOU is 0.5144.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5514224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046560PMC
April 2021

Exenatide twice daily plus glargine vs. aspart 70/30 twice daily in type 2 diabetic patients with inadequate glycaemic control on premixed human insulin and metformin.

Endocr Pract 2021 Apr 5. Epub 2021 Apr 5.

Division of Endocrinology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Background: Many type 2 diatetic patients treated with premixed insulin gradually have inadequate glycaemic control and switch to a basal-bolus regimen, which raises some concerns for weight gain and increased hypoglycaemic risk. Switching to combination use of glp-1 agonist and basal insulin may be an alternative option.

Methods: After a 12-week premixed human insulin 70/30 dosage optimization period, 200 patients with HbA1c of 7.0% to 10.0% were randomized into 24-week treatment groups with exenatide BID plus glargine or with aspart 70/30 BID.

Results: After 24 weeks, the patients receiving exenatide plus glargine (n = 90) had improved HbA1c control compared with those receiving aspart 70/30 (n = 90) (least squares mean change: -0.59 vs. -0.13%; difference [95% CI]: -0.45 [-0.74 to -0.17]) in the full analysis set population. Weight decreased 3.5 kg with exenatide and decreased 0.4 kg with aspart 70/30 (P < 0.001). The insulin dose was reduced 10.7 units/day (95% CI, -12.2 to -9.2 units; P < 0.001) with exenatide, and increased 9.7 units/day (95% CI, 8.2 to 11.2 units; P < 0.001) with aspart 70/30. The most common adverse events were gastrointestinal adverse effects in the exenatide group [nausea (21%), vomiting (16%), diarrhea (13%)]. The incidence of hypoglycaemia was similar in two groups (27% for exenatide and 38% for aspart 70/30, respectively; P = 0.1).

Conclusions: In premixed human insulin-treated type 2 diabetic patients with inadequate glycaemic control, switching to exenatide BID plus glargine was superior to aspart 70/30 BID for glycaemic and weight control.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.eprac.2021.03.015DOI Listing
April 2021

Eriodictyol inhibits glioblastoma migration and invasion by reversing EMT via downregulation of the P38 MAPK/GSK-3β/ZEB1 pathway.

Eur J Pharmacol 2021 Jun 31;900:174069. Epub 2021 Mar 31.

Department of Pharmacy, The Third Affiliated Hospital of Chongqing Medical University (Gener Hospital), Chongqing, 401120, PR China. Electronic address:

Eriodictyol (ERD) is a natural flavonoid that exists in many vegetables and fruits, especially citrus fruits. It has been proven to have many pharmacological effects, such as antioxidative, anti-inflammatory and neuroprotective effects. Our previous study showed that eriodictyol could inhibit the proliferation and induce the apoptosis of glioblastoma cells by downregulating the PI3K/Akt/NF-κB pathway and restraining its migration and invasion. However, the mechanism by which eriodictyol prevents glioblastoma metastasis is still unknown. Epithelial-mesenchymal transition (EMT) is a key process for many cancer metastases; it also confers locomotivity to tumor cells, including glioblastoma. In this study, we found that eriodictyol can suppress the migration and invasion of glioblastoma A172 and U87 MG cell lines by suppressing the EMT markers - N-cadherin and E-cadherin through Wound healing and Transwell assays, Western blot, RT-qPCR, immunofluorescence and immunohistochemistry. Further research revealed that the mechanism could be connected with downregulation of the P38 MAPK/GSK-3β/ZEB1 signaling pathway. These findings can provide a new idea for the treatment of glioblastoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejphar.2021.174069DOI Listing
June 2021

Confining copper nanoclusters on exfoliation-free 2D boehmite nanosheets: Fabrication of ultra-sensitive sensing platform for α-glucosidase activity monitoring and natural anti-diabetes drug screening.

Biosens Bioelectron 2021 Jun 26;182:113198. Epub 2021 Mar 26.

College of Chemistry, Jilin University, Changchun, 130012, China; Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Sciences, Jilin University, Changchun, 130012, China. Electronic address:

α-Glucosidase (α-Glu) and its inhibitors play critical roles in diabetes therapy. Herein, a simple and ultra-sensitive fluorescence sensing approach was fabricated for α-Glu activity monitoring and natural inhibitor screening by electrostatically confining negatively charged glutathione-capped copper nanoclusters (GSH-CuNCs) on exfoliation-free and positively charged 2D boehmite (Boe) nanosheets. Boe significantly improved the fluorescence emission/stability of GSH-CuNCs and simultaneously led to an obvious blue-shift of the excitation peak of CuNCs from 365 nm to 330 nm. As a result, the fluorescence emission of [email protected] was efficiently quenched by 4-nitrophenyl-α-D-glucopyranoside (PNPG) with a maximum absorbance peak (λ) at 310 nm via inner filter effect, and sequentially recovered by α-Glu through the hydrolysis of PNPG to p-nitrophenol (λ = 410 nm). Accordingly, an ultra-sensitive fluorescence assay for the determination of α-Glu activity was proposed by using [email protected] as fluorescence probes. The detection limit of 0.43 U/L was achieved, which was lower than most of other α-Glu activity assays. Furthermore, this method was capable of screening α-Glu inhibitors originated from actinomycetes, peanut, sophora flower, celery, and orange as potential anti-diabetes drugs. Taken together, this work provided a promising strategy for clinical treatment of diabetes and discovery of anti-diabetes drugs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2021.113198DOI Listing
June 2021

Casting Light on the Adaptation Mechanisms and Evolutionary History of the Widespread Sumerlaeota.

mBio 2021 03 30;12(2). Epub 2021 Mar 30.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, China

Sumerlaeota is a mysterious, putative phylum-level lineage distributed globally but rarely reported. As such, their physiology, ecology, and evolutionary history remain unknown. The 16S rRNA gene survey reveals that Sumerlaeota is frequently detected in diverse environments globally, especially cold arid desert soils and deep-sea basin surface sediments, where it is one dominant microbial group. Here, we retrieved four Sumerlaeota metagenome-assembled genomes (MAGs) from two hot springs and one saline lake. Including another 12 publicly available MAGs, they represent six of the nine putative Sumerlaeota subgroups/orders, as indicated by 16S rRNA gene-based phylogeny. These elusive organisms likely obtain carbon mainly through utilization of refractory organics (e.g., chitin and cellulose) and proteinaceous compounds, suggesting that Sumerlaeota act as scavengers in nature. The presence of key bidirectional enzymes involved in acetate and hydrogen metabolisms in these MAGs suggests that they are acetogenic bacteria capable of both the production and consumption of hydrogen. The capabilities of dissimilatory nitrate and sulfate reduction, nitrogen fixation, phosphate solubilization, and organic phosphorus mineralization may confer these heterotrophs great advantages to thrive under diverse harsh conditions. Ancestral state reconstruction indicated that Sumerlaeota originated from chemotrophic and facultatively anaerobic ancestors, and their smaller and variably sized genomes evolved along dynamic pathways from a sizeable common ancestor (2,342 genes), leading to their physiological divergence. Notably, large gene gain and larger loss events occurred at the branch to the last common ancestor of the order subgroup 1, likely due to niche expansion and population size effects. In recent years, the tree of life has expanded substantially. Despite this, many abundant yet uncultivated microbial groups remain to be explored. The candidate phylum Sumerlaeota is widely distributed in various harsh environments. However, their physiology, adaptation mechanisms, and evolution remain elusive due to a lack of pure cultures and limited available genomes. Here, we used genomes from uncultivated members of Sumerlaeota to disclose why these taxa can thrive under diverse harsh conditions and how they evolved from a chemotrophic and facultatively anaerobic common ancestor. This study deeply explored the biology of Sumerlaeota and provided novel insights into their possible roles in global biogeochemical cycles, adaptation mechanisms, ecological significance, and evolutionary history.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/mBio.00350-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092238PMC
March 2021

Aquiflexum lacus sp. nov., isolated from a lake sediment sample.

Arch Microbiol 2021 Mar 24. Epub 2021 Mar 24.

State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People's Republic of China.

A novel Gram-staining negative, crescent-like or rod-shaped, non-motile bacterium, designated strain CUG 91378, was isolated from a sediment sample of Qinghai Lake, Qinghai Province, China. The strain was red-colored, and catalase- and oxidase-positive. Strain CUG 91378 was able to grow at 15-37 °C (optimum, 28 °C), pH 7-9 (pH 7.0) and in the presence of up to 3.0% (w/v) NaCl (0-2%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CUG 91378 formed a well-supported monophyletic clade with Aquiflexum balticum DSM 16537 (95.4%) and Aquiflexum aquatile Z0201 (93.2%). The DNA G + C content of CUG 91378 was 39.0%. Low (< 87%) average nucleotide identity (ANI) and (< 26%) digital DNA-DNA hybridization (dDDH) values were observed between strain CUG 91378 and its closest species on the phylogenetic trees. The sole respiratory quinone of strain CUG 91378 was MK-7. The predominant fatty acids (> 5.0%) were iso-C (19.1%), iso-C (12.0%), iso-C H (10.9%), iso-C 3OH (9.2%), iso-C 3OH (7.7%), Cω6c (6.1%) and anteiso-C (5.8%). Strain CUG 91378 contained as phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and four unidentified lipids (L1, L2, L3 and L4). Based on the data from the current polyphasic study, the isolate represents a novel species of the genus Aquiflexum for which the name Aquiflexum lacus is proposed. The type strain of the proposed new taxon is CUG 91378 (= KCTC 62637 = CGMCC 1.13988).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00203-021-02280-zDOI Listing
March 2021

A preliminary study on pollution characteristics of surfactant substances in fine particles in the Beibu Gulf Region of China.

J Environ Sci (China) 2021 Apr 20;102:363-372. Epub 2020 Oct 20.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100021, China.

The pollution characteristics of surfactant substances in fine particles (PM) in spring were studied in the Beibu Gulf Region of China, 68 samples of PM were collected at Weizhou Island in Beihai City from March 12 to April 17, 2015. The Anionic Surfactant Substances (ASS) and Cationic Surfactant Substances (CSS) in the samples were analyzed using Byethyl Violet Spectrophotometry and Disulfide Blue Spectrophotometry, respectively. Combined with the data from backward trajectory simulation, the effects of air pollutants from remote transport on the pollution characteristics of surfactant substances in PM in the Beibu Gulf Region were analyzed and discussed. The results showed that the daily mean concentrations of ASS and CSS in spring in the Beibu Gulf Region were 165.20 pmol/m and 8.05pmol/m, and the variation ranges were 23.21-452.55 pmol/m and 0.65-31.31 pmol/m, accounting for 1.82‰ ± 1.65‰ and 0.12‰ ± 0.11‰ of the mass concentration of PM, respectively. These concentrations were lower than those in comparable regions around the world. There was no clear correlation between the concentrations of ASS and CSS in PM and the mass concentrations of PM. Tourism and air transport had a positive contribution on the concentrations of ASS. The concentration of surfactant substances in PM was significantly impacted by wind speed and wind direction. Atmospheric temperature, air pressure and precipitation had little effect on the concentrations of surfactant substances. Surfactant substances in PM significantly impacted visibility. Results also showed that the main sources of surfactant substances were from the southern China and Southeast Asia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jes.2020.08.008DOI Listing
April 2021

Modeling driver behavior in the dilemma zone based on stochastic model predictive control.

PLoS One 2021 24;16(2):e0247453. Epub 2021 Feb 24.

Department of Traffic and Transportation, Jilin University, Changchun, China.

Driver behavior is considered one of the most important factors in the genesis of dilemma zones and the safety of driver-vehicle-environment systems. An accurate driver behavior model can improve the traffic signal control efficiency and decrease traffic accidents in signalized intersections. This paper uses a mathematical modeling method to study driver behavior in a dilemma zone based on stochastic model predictive control (SMPC), along with considering the dynamic characteristics of human cognition and execution, aiming to provide a feasible solution for modeling driver behavior more accurately and potentially improving the understanding of driver-vehicle-environment systems in dilemma zones. This paper explores the modeling framework of driver behavior, including the perception module, decision-making module, and operation module. The perception module is proposed to stimulate the ability to perceive uncertainty and select attention in the dilemma zone. An SMPC-based driver control modeling method is proposed to stimulate decision-making behavior in the dilemma zone. The operation module is proposed to stimulate the execution ability of the driver. Finally, CarSim, the well-known vehicle dynamics analysis software package, is used to verify the proposed models of this paper. The simulation results show that the SMPC-based driver behavior model can effectively and accurately reflect the vehicle motion and dynamics under driving in the dilemma zone.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247453PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904219PMC
February 2021

Crosstalk between nonclassical monocytes and alveolar macrophages mediates transplant ischemia-reperfusion injury through classical monocyte recruitment.

JCI Insight 2021 Mar 22;6(6). Epub 2021 Mar 22.

Division of Thoracic Surgery and.

Primary graft dysfunction (PGD) is the predominant cause of early graft loss following lung transplantation. We recently demonstrated that donor pulmonary intravascular nonclassical monocytes (NCM) initiate neutrophil recruitment. Simultaneously, host-origin classical monocytes (CM) permeabilize the vascular endothelium to allow neutrophil extravasation necessary for PGD. Here, we show that a CCL2-CCR2 axis is necessary for CM recruitment. Surprisingly, although intravital imaging and multichannel flow cytometry revealed that depletion of donor NCM abrogated CM recruitment, single cell RNA sequencing identified donor alveolar macrophages (AM) as predominant CCL2 secretors. Unbiased transcriptomic analysis of murine tissues combined with murine KOs and chimeras indicated that IL-1β production by donor NCM was responsible for the early activation of AM and CCL2 release. IL-1β production by NCM was NLRP3 inflammasome dependent and inhibited by treatment with a clinically approved sulphonylurea. Production of CCL2 in the donor AM occurred through IL-1R-dependent activation of the PKC and NF-κB pathway. Accordingly, we show that IL-1β-dependent paracrine interaction between donor NCM and AM leads to recruitment of recipient CM necessary for PGD. Since depletion of donor NCM, IL-1β, or IL-1R antagonism and inflammasome inhibition abrogated recruitment of CM and PGD and are feasible using FDA-approved compounds, our findings may have potential for clinical translation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1172/jci.insight.147282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026186PMC
March 2021

Differences in Performance of Immunosensors Constructed Based on CeO-Simulating Auxiliary Enzymes.

ACS Biomater Sci Eng 2021 03 22;7(3):1058-1064. Epub 2021 Feb 22.

School of Chemistry and Chemical Engineering, Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, No. 221, Beisi Road, Xinjiang Uygur Autonomous Region, Shihezi 832000, China.

The morphology effect of cerium oxide (CeO) has always been the focus of catalysis research. Few people have reported the relationship between the morphology of CeO and electrochemical performance in sensors. In this paper, a polyaniline (PANI) matrix is used as the dispersant and stabilizer, ultrafine Au nanoparticles (NPs) ([email protected]) are uniformly embedded in the PANI matrix, and Au [email protected] is fixed on the surface of CeO with different morphologies and sizes (Spindle CeO:(SCe), octahedron CeO (OCe)). The morphology and crystal structure of CeO were adjusted under different ratios of ethanol and water, and the effect of CeO was evaluated. The synthesized [email protected] has different morphologies, sizes, and electrochemical properties. The electrochemical catalytic behavior of [email protected] was studied by using hydrogen peroxide (HO) as the reaction substrate. The instantaneous current method (-) was used to further study the electrochemical amplification effect, and the best performance was obtained.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsbiomaterials.0c01680DOI Listing
March 2021

Synergistic effects of Indian hedgehog and sonic hedgehog on chondrogenesis during cartilage repair.

J Mol Histol 2021 Apr 17;52(2):407-418. Epub 2021 Feb 17.

Department of Orthopaedics, Shanghai Tenth People's Hospital, Tongji University, Shanghai, 200072, China.

Sonic hedgehog (Shh) and Indian hedgehog (Ihh) have been shown to control the induction of early cartilaginous differentiation. However, it is unclear whether Ihh and Shh exert synergistic effects on chondrogenesis during articular cartilage repair. Herein, we investigate the effects of chondrogenesis of bone-derived mesenchymal stem cells (BMSCs) following co-transfection with Shh and Ihh via adenoviral vectors in vitro and in vivo. A rotary cell culture system (RCCS) and Cytodex 3 microcarriers were used to create a stereoscopic dynamic environment for cell culture. In the RCCS environment, BMSCs co-transfected with Ihh and Shh displayed stronger chondrogenic differentiation and chondrogenesis than BMSCs transfected with Ihh or Shh alone, and exhibited higher expression levels of Sox 9, ACAN and collagen II, stronger toluidine blue and collagen II immunohistochemical staining. After transplanted into the osteochondral defect at 8 weeks, Ihh/Shh co-transfected BMSCs showed a significantly better cartilage repair than BMSCs transfected with Ihh or Shh alone. Ihh and Shh have synergistic effects on the induction of chondrogenic differentiation and chondrogenesis under a microgravity environment, and help to repair damaged cartilage and reverse subchondral defects during the early stages.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10735-021-09964-2DOI Listing
April 2021

The Rice Small Auxin-Up RNA Gene Regulates Seed Vigor via Sugar Pathway during Early Seed Germination.

Int J Mol Sci 2021 Feb 4;22(4). Epub 2021 Feb 4.

The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, Guangdong Laboratory of Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.

Seed vigor affects seed germination and seedling emergence, and therefore is an important agronomic trait in rice. Small auxin-up RNAs () function in a range of developmental processes, but their role in seed vigor remains unclear. Here, we observed that disruption of resulted in reduced germination rates and low seed uniformity in early germination. Expression of was higher in mature grains and early germinating seeds. RNA-seq analysis revealed that modulated seed vigor by affecting the mobilization of stored reserves during germination. Disruption of increased the soluble sugar content in dry mature grains and seeds during early germination. OsSAUR33 interacted with the sucrose non-fermenting-1-related protein kinase OsSnRK1A, a regulator of the sugar signaling pathway, which influences the expression of sugar signaling-related genes during germination. Disruption of increased sugar-sensitive phenotypes in early germination, suggesting OsSAUR33 likely affects seed vigor through the sugar pathway. One elite haplotype of associated with higher seed vigor was identified mainly in accessions. This study provides insight into the effects of OsSAUR33 on seed vigor in rice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms22041562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913900PMC
February 2021

Dihydromyricetin Alleviates High Glucose-Induced Oxidative Stress and Apoptosis in Human Retinal Pigment Epithelial Cells by Downregulating miR-34a Expression.

Diabetes Metab Syndr Obes 2021 27;14:387-397. Epub 2021 Jan 27.

Department of Ophthalmology, Jingmen No. 2 People's Hospital, Jingmen 448000, People's Republic of China.

Background: Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes mellitus, which leads to neuronal and vascular dysfunction in the retina with a final outcome of complete loss of vision. The aim of the present study was to investigate the effects of dihydromyricetin (DHM), a natural flavanol compound, on diabetic retinopathy (DR) and identify its potential mechanisms.

Methods: Retinal pigment epithelial cell line (ARPE-19) treated with high glucose (HG) was used to simulate the DR model in vitro. After treatment with different concentrations of DHM, the cell viability, production of reactive oxygen species (ROS) and the levels of oxidative stress-related markers in the in vitro model were detected using corresponding kits. Cell apoptosis was determined using terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) staining, and the expression of apoptotic proteins was examined using Western blot analysis. Subsequently, microRNA (miR)-34a expression was measured by reverse transcription-quantitative PCR (RT-qPCR). The levels of oxidative stress and apoptosis were evaluated after miR-34a overexpression.

Results: Results indicated that DHM dose-dependently elevated the decreased cell viability induced by HG. Moreover, the content of ROS was significantly reduced in HG-stimulated ARPE-19 cells, accompanied by enhanced activities of superoxide dismutase (SOD) and catalase (CAT) antioxidases, as well as concentration of glutathione (GSH). Furthermore, remarkably decreased apoptosis of ARPE-19 cells induced by HG was observed following DHM intervention. Importantly, HG stimulation notably upregulated miR-34a expression, which was reversed by DHM treatment. Importantly, the inhibitory effects of DHM on HG-induced oxidative stress and apoptosis of ARPE-19 cells were restored following miR-34a overexpression.

Conclusion: Taken together, this work demonstrated that DHM exerts protective effects on HG-induced oxidative stress and apoptotic damage in ARPE-19 cells via inhibition of miR-34a expression, providing a promising therapeutic agent for the treatment of DR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/DMSO.S290633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850407PMC
January 2021

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration.

Front Bioeng Biotechnol 2020 13;8:595096. Epub 2021 Jan 13.

Department of Stomatology, First Affiliated Hospital, Dalian Medical University, Dalian, China.

Native dental pulp extracellular matrix (DPEM) has proven to be an effective biomaterial for dental pulp regeneration. However, as a significant extracellular matrix glycoprotein, partial laminins were lost during the decellularization process, which were essential for odontoblast differentiation. Thereby, this study investigated the feasibility of LN supplementation to improve the surface of DPEM for odontoblast layer regeneration. The influences of laminin on cell adhesion and odontogenic differentiation were evaluated in vitro. Then, we fabricated laminin-modified DPEM based on the physical coating strategy and observed the location and persistency of laminin coating by immunofluorescent staining. Finally, laminin-modified DPEM combined with treated dentin matrix (TDM) was transplanted in orthotopic jaw bone of beagles ( = 3) to assess the effect of LNs on dental pulp tissue regeneration. The in vitro results showed that laminins could improve the adhesion of dental pulp stem cells (DPSCs) and promoted DPSCs toward odontogenic differentiation. Continuous odontoblastic layer-like structure was observed in laminin-modified DPEM group, expressing the markers for odontoblastogenesis, dentine matrix protein-1 (DMP-1) and dentin sialophosphoprotein (DSPP). Overall, these studies demonstrate that the supplementation of laminins to DPEM contributes to the odontogenic differentiation of cells and to the formation of odontoblast layer in dental pulp regeneration.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2020.595096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838611PMC
January 2021

Ultrasensitive electrochemical immunosensor based on the signal amplification strategy of the competitive reaction of Zn and ATP ions to construct a "signal on" mode GOx-HRP enzyme cascade reaction.

Mikrochim Acta 2021 Jan 29;188(2):61. Epub 2021 Jan 29.

Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Production and Construction Corps, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, 832003, People's Republic of China.

A GOx/[email protected] nanomaterial is proposed by coating GOx and HRP in ZIF-90 using a bio-simulated mineralization method to improve the tolerance of the enzyme to the external environment. In the detection process, the ZIF-90 is turned on under mild conditions by the competitive reaction of ATP with Zn and imidazole-2-carboxaldehyde (2-ICA), and the electrical signal of the system is amplified by the enzyme cascade reaction of GOx and HRP. Finally, based on the signal amplification strategy of the competitive reaction between Zn and ATP to construct a "signal on" mode, electrochemical immunosensor of GOx-HRP enzyme-linked cascade reaction was prepared. The proposed electrochemical immunosensor shows an excellent analytical performance when detecting CA-125, with good selectivity and stability, with a detection range of 0.1 pg mL-40 ng mL and a detection limit of 0.05 pg mL. The test has been performed using chronoamperometry under a constant voltage of -0.4 V. The immunosensor also shows an excellent performance when analysing human blood samples. The recovery of the immunosensor is 97.94-101.8%, with a relative standard deviation of 3.7-6.1%. The proposed sensor provides a novel idea for clinical use of GOx and HRP enzymes and a new method for the clinical detection of tumor markers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-021-04720-5DOI Listing
January 2021

Detection of Snore from OSAHS Patients Based on Deep Learning.

J Healthc Eng 2020 12;2020:8864863. Epub 2020 Dec 12.

The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang, China.

Obstructive sleep apnea-hypopnea syndrome (OSAHS) is extremely harmful to the human body and may cause neurological dysfunction and endocrine dysfunction, resulting in damage to multiple organs and multiple systems throughout the body and negatively affecting the cardiovascular, kidney, and mental systems. Clinically, doctors usually use standard PSG (Polysomnography) to assist diagnosis. PSG determines whether a person has apnea syndrome with multidimensional data such as brain waves, heart rate, and blood oxygen saturation. In this paper, we have presented a method of recognizing OSAHS, which is convenient for patients to monitor themselves in daily life to avoid delayed treatment. Firstly, we theoretically analyzed the difference between the snoring sounds of normal people and OSAHS patients in the time and frequency domains. Secondly, the snoring sounds related to apnea events and the nonapnea related snoring sounds were classified by deep learning, and then, the severity of OSAHS symptoms had been recognized. In the algorithm proposed in this paper, the snoring data features are extracted through the three feature extraction methods, which are MFCC, LPCC, and LPMFCC. Moreover, we adopted CNN and LSTM for classification. The experimental results show that the MFCC feature extraction method and the LSTM model have the highest accuracy rate which was 87% when it is adopted for binary-classification of snoring data. Moreover, the AHI value of the patient can be obtained by the algorithm system which can determine the severity degree of OSAHS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/8864863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787852PMC
December 2020

Individual Social Capital and Life Satisfaction among Mainland Chinese Adults: Based on the 2016 China Family Panel Studies.

Int J Environ Res Public Health 2021 01 8;18(2). Epub 2021 Jan 8.

Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, No. 1163 Xinmin Street, Changchun 130021, China.

Background: At present, most Chinese adults are under great psychological pressure, which seriously affects the improvement of life satisfaction. The purpose of this study was to shed light on the associations between individual social capital and life satisfaction among mainland Chinese adults.

Methods: We used a nationally representative dataset called 2016 China Family Panel Studies, and a total of 26,009 people suited our study requirements. Chi-squared test and binary regression analysis were used to determine the relationship between individual social capital and life satisfaction among mainland Chinese adults.

Results: The results indicated that cognitive social capital had significant effects on life satisfaction ( < 0.05), but the impact of structural social capital on life satisfaction became less significant when combined with sociodemographic variables and socioeconomic status (SES) ( > 0.05). Moreover, we also found that life satisfaction was better in married/cohabited ( < 0.05) and in over 65 age group people ( < 0.05). Self-rated income level, self-rated social status, and self-rated health all had positive effects on life satisfaction ( < 0.001).

Conclusions: Given the stabilizing effect of cognitive social capital at the individual level on life satisfaction, the government should attach great importance to this aspect when trying to improve adults' life satisfaction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph18020441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827718PMC
January 2021

In vivo evaluation of bending strengths and degradation rates of different magnesium pin designs for oral stapler.

J Appl Biomater Funct Mater 2020 Jan-Dec;18:2280800019836400

Center of Digital Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.

Magnesium alloys have been potential biodegradable implants in the areas of bone, cardiovascular system, gastrointestinal tract, and so on. The purpose of this study is to evaluate Mg-2Zn alloy degradation as a potential suture material. The study included Sprague-Dawley (SD) rats in vivo. In 24 male SD rats, tests in the leg muscle were conducted using traditional surgical incision and insertion of magnesium alloys of different designs into the tissue. The material degradation topography, elemental composition, and strength of the pins were analyzed. This paper explores magnesium pins with different cross-sectional shapes and diameters to establish a suitable pin diameter and shape for use as an oral stapler, which must have a good balance of degradation rate and strength. The results showed there were good bending strengths over different degradation periods in groups with diameters of 0.8 mm and 0.5 mm, and no significantly different bending strength between the groups of triangle and round cross-section shapes with same diameter of 0.3 mm, although the degradation rate still needs to be improved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/2280800019836400DOI Listing
December 2020

[Differential diagnosis of high altitude pulmonary edema and COVID-19 with computed tomography feature].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2020 Dec;37(6):1031-1036

Department of Diagnostic Radiology, Xinjiang General Hospital of PLA, Urumqi 830000, P.R.China.

To investigate the computed tomography (CT) characteristics and differential diagnosis of high altitude pulmonary edema (HAPE) and COVID-19, CT findings of 52 cases of HAPE confirmed in Medical Station of Sanshili Barracks, PLA 950 Hospital from May 1, 2020 to May 30, 2020 were collected retrospectively. The size, number, location, distribution, density and morphology of the pulmonary lesions of these CT data were analyzed and compared with some already existed COVID-19 CT images which come from two files, "Radiological diagnosis of COVID-19: expert recommendation from the Chinese Society of Radiology (First edition)" and "A rapid advice guideline for the diagnosis and treatment of 2019 novel corona-virus (2019-nCoV) infected pneumonia (standard version)". The simple or multiple ground-glass opacity (GGO) lesions are located both in the HAPE and COVID-19 at the early stage, but only the thickening of interlobular septa, called "crazy paving pattern" belongs to COVID-19. At the next period, some increased cloudy shadows are located in HAPE, while lesions of COVID-19 are more likely to develop parallel to the direction of the pleura, and some of the lesions show the bronchial inflation. At the most serious stage, both the shadows in HAPE and COVID-19 become white, but the lesions of HAPE in the right lung are more serious than that of left lung. In summary, some cloudy shadows are the feature of HAPE CT image, and "crazy paving pattern" and "pleural parallel sign" belong to the COVID-19 CT, which can be used for differential diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7507/1001-5515.202007043DOI Listing
December 2020