Publications by authors named "Wenjuan Zhang"

426 Publications

Risk of migraine contributed by genetic polymorphisms of ANKDD1B gene: a case-control study based on Chinese Han population.

Neurol Sci 2021 Oct 20. Epub 2021 Oct 20.

Department of Forensic Medicine, School of Medicine & Forensics, Xi'an Jiaotong University Health Science Center, 76 Yanta West Road, Xi'an, 710061, Shaanxi, China.

Early studies have indicated that the risk of migraine is contributed by both genetic and environmental factors. We aimed to evaluate the association between the risk of migraine and genetic polymorphisms in the ANKDD1B gene in a large sample of Chinese Han populations. A total of 882 patients with MO and 1,784 age-matched controls were recruited. A list of 12 tag SNPs located within the ANKDD1B gene region was genotyped. Distributions of SNP genotypes and alleles between patients and controls were examined to investigate the associations between the risk of migraine and genetic polymorphisms. The GTEx database was used to examine the effects of the significant SNPs on gene expressions. A stop-gain SNP, rs34358, was discovered to be significantly related with the risk of migraine (χ = 25.02, P = 5.66 × 10). The A allele of this SNP was significantly associated with a decreased risk of migraine (OR [95% CI] = 0.73 [0.65-0.83]). A dose-dependent pattern was identified in the genotypic analyses. The OR with 95% confidence interval for genotype AA versus GG was 0.55 [0.42-0.72], while for AG versus GG it was 0.74 [0.62-0.88]. Further bioinformatics analysis showed multiple significant signals (20 out of 47) for the association between SNP rs34358 and gene expression levels of ANKDD1B. In conclusion, we have provided population-based evidence for the association between genetic polymorphisms of the ANKDD1B gene and the risk of migraine. A protein-truncating variant was significantly associated with a decreased risk of migraine in the samples recruited from the Chinese Han population.
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http://dx.doi.org/10.1007/s10072-021-05645-wDOI Listing
October 2021

Kaempferide enhances antioxidant capacity to promote osteogenesis through FoxO1/β-catenin signaling pathway.

Eur J Pharmacol 2021 Oct 7;911:174555. Epub 2021 Oct 7.

Lab for Bone Metabolism, Xi'an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China. Electronic address:

Background: Forkhead box O1 (FoxO1)/β-catenin signaling pathway is a main oxidative defense pathway, which plays essential roles in the regulation of osteoporosis (OP). The natural products possess quality therapeutic effects and few side effects. It is used as a novel strategy in the treatment of OP. However, there is no systematic study in the natural antioxidant drug based on the FoxO1/β-catenin signaling pathway. This paper aims to discover pro-osteogenesis natural antioxidants for the prevention and treatment of OP.

Methods: Systems pharmacology; combined with reverse drug targeting, systems-ADME process, network analysis and molecular docking, was used to screen natural antioxidants based on the FoxO1/β-catenin signaling pathway. Then in vitro experiments were performed to evaluate the osteogenesis effects of screened natural antioxidants.

Results: Kaempferide was screened as the most potential antioxidant to improve osteogenesis by the regulation of the FoxO1/β-catenin signaling pathway. In vitro experiments showed that kaempferide significantly increased the expression of antioxidant genes and promoted osteogenic differentiation. Furthermore, kaempferide also improved the osteogenic differentiation inhibited by HO through the enhancement of antioxidant capacity. Notably, kaempferide promoted cell antioxidant capacity by the increased nuclear translocation of FoxO1 and β-catenin.

Conclusions: These findings suggest that kaempferide is the natural antioxidant to promote osteogenesis effectively through the FoxO1/β-catenin signaling pathway. Natural antioxidant therapy maybe a promising strategy for the prevention and treatment of OP.
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http://dx.doi.org/10.1016/j.ejphar.2021.174555DOI Listing
October 2021

Food-Origin Mycotoxin-Induced Neurotoxicity: Intend to Break the Rules of Neuroglia Cells.

Oxid Med Cell Longev 2021 28;2021:9967334. Epub 2021 Sep 28.

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Jinjing Road No. 22, Xiqing District, Tianjin 300384, China.

Mycotoxins are key risk factors in human food and animal feed. Most of food-origin mycotoxins could easily enter the organism and evoke systemic toxic effects, such as aflatoxin B1 (AFB1), ochratoxin A (OTA), T-2 toxin, deoxynivalenol (DON), zearalenone (ZEN), fumonisin B1 (FB1), and 3-nitropropionic acid (3-NPA). For the last decade, the researches have provided much evidences in vivo and in vitro that the brain is an important target organ on mycotoxin-mediated neurotoxic phenomenon and neurodegenerative diseases. As is known to all, glial cells are the best regulator and defender of neurons, and a few evaluations about the effects of mycotoxins on glial cells such as astrocytes or microglia have been conducted. The fact that mycotoxin contamination may be a key factor in neurotoxicity and glial dysfunction is exactly the reason why we reviewed the activation, oxidative stress, and mitochondrial function changes of glial cells under mycotoxin infection and summarized the mycotoxin-mediated glial cell proliferation disorders, death pathways, and inflammatory responses. The purpose of this paper is to analyze various pathways in which common food-derived mycotoxins can induce glial toxicity and provide a novel perspective for future research on the neurodegenerative diseases.
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http://dx.doi.org/10.1155/2021/9967334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492254PMC
September 2021

Structure-based classification of tauopathies.

Nature 2021 10 29;598(7880):359-363. Epub 2021 Sep 29.

MRC Laboratory of Molecular Biology, Cambridge, UK.

The ordered assembly of tau protein into filaments characterizes several neurodegenerative diseases, which are called tauopathies. It was previously reported that, by cryo-electron microscopy, the structures of tau filaments from Alzheimer's disease, Pick's disease, chronic traumatic encephalopathy and corticobasal degeneration are distinct. Here we show that the structures of tau filaments from progressive supranuclear palsy (PSP) define a new three-layered fold. Moreover, the structures of tau filaments from globular glial tauopathy are similar to those from PSP. The tau filament fold of argyrophilic grain disease (AGD) differs, instead resembling the four-layered fold of corticobasal degeneration. The AGD fold is also observed in ageing-related tau astrogliopathy. Tau protofilament structures from inherited cases of mutations at positions +3 or +16 in intron 10 of MAPT (the microtubule-associated protein tau gene) are also identical to those from AGD, suggesting that relative overproduction of four-repeat tau can give rise to the AGD fold. Finally, the structures of tau filaments from cases of familial British dementia and familial Danish dementia are the same as those from cases of Alzheimer's disease and primary age-related tauopathy. These findings suggest a hierarchical classification of tauopathies on the basis of their filament folds, which complements clinical diagnosis and neuropathology and also allows the identification of new entities-as we show for a case diagnosed as PSP, but with filament structures that are intermediate between those of globular glial tauopathy and PSP.
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http://dx.doi.org/10.1038/s41586-021-03911-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7611841PMC
October 2021

Brain distribution of berzosertib: an ATR inhibitor for the treatment of glioblastoma.

J Pharmacol Exp Ther 2021 Sep 23. Epub 2021 Sep 23.

Pharmaceutics, University of Minnesota, United States

The effective treatment of brain tumors is a considerable challenge in part due to the presence of the blood-brain barrier (BBB) that limits drug delivery. Glioblastoma (GBM) is an aggressive and infiltrative primary brain tumor with an extremely poor prognosis following standard of care therapy with surgery, radiation therapy (RT), and chemotherapy. DNA damage response (DDR) pathways play a critical role in DNA repair in cancer cells, and inhibition of these pathways can potentially augment RT and chemotherapy tumor cell toxicity. The Ataxia Telangiectasia and Rad3-Related (ATR) kinase is a key regulator of the DDR network and is potently and selectively inhibited by the ATR inhibitor, berzosertib. While in vitro studies demonstrate a synergistic effect of berzosertib in combination with temozolomide (TMZ), in vivo efficacy studies have yet to recapitulate this observation using intracranial tumor models. In the current study, we demonstrate that delivery of berzosertib to the brain is restricted by efflux at the BBB. Berzosertib has a high binding affinity to brain tissue compared to plasma, thereby leading to low free drug concentrations in the brain. Berzosertib distribution is heterogenous within the tumor, where concentrations are substantially lower in normal brain and invasive tumor rim (where the BBB is intact) when compared to the tumor core (where the BBB is leaky). These results demonstrate that high tissue binding and limited and heterogenous brain distribution of berzosertib may be important factors that influence the efficacy of berzosertib therapy in GBM. This study examined the brain delivery and efficacy of the potent ATR inhibitor - berzosertib, in PDX models of GBM. Berzosertib is actively effluxed at the BBB and is highly bound to brain tissue leading to low free drug concentrations in the brain. Berzosertib is heterogeneously distributed into different regions of the brain and tumor and was not efficacious in vivo when combined with TMZ. These factors inform the future clinical utility of berzosertib for GBM.
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http://dx.doi.org/10.1124/jpet.121.000845DOI Listing
September 2021

Aptamer-gold nanoparticle-signal probe bioconjugates amplify electrochemical signal for the detection of prostate specific antigen.

Anal Methods 2021 Sep 23;13(36):4150-4156. Epub 2021 Sep 23.

College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Normal University, Wuhu 241000, People's Republic of China.

In this study, we reported a simple and sensitive electrochemical immunosensor for the detection of PSA, a prostate cancer biomarker. In the design protocol, gold nanoparticles (Au NPs) were used a carrier to load an aptamer and the binding DNA labeled with methylene blue (MB, signal probe) for signal amplification (denoted as aptamer-Au NP-signal probe bioconjugate). The immunosensor was fabricated by immobilizing antibodies on the electrode surface modified with Au NPs to capture the PSA antigen, and then sandwiched with the aptamer-Au NP-signal probe (AASp) bioconjugates. Square wave voltammetry (SWV) was employed to record the detection signal in phosphate-buffered solution (PBS, pH 7.4). As a result, a well-shaped peak was obtained at about -0.45 V ( SCE) corresponding to the oxidation of MB, and the peak intensity was related to the concentration of PSA. Because of the amplification of the detection signal by the as-synthesized AASp bioconjugates, the immunosensor achieved a wide linear response range (0.001 to 75.0 ng mL) and a low detection limit of 3.0 pg mL (at S/N = 3). Further, the immunoassay exhibited excellent selectivity.
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http://dx.doi.org/10.1039/d1ay01175hDOI Listing
September 2021

Circular RNA WHSC1 exerts oncogenic properties by regulating miR-7/TAB2 in lung cancer.

J Cell Mol Med 2021 Oct 22;25(20):9784-9795. Epub 2021 Sep 22.

Department of Geriatrics, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Circular RNA is a newly discovered member of non-coding RNA (ncRNA) and regulates the target gene by acting as a micro-RNA sponge. It plays vital roles in various diseases. However, the functions of circular RNA in non-small cell lung cancer (NSCLC) remain still unclear. Our data showed that circ-WHSC1 was highly expressed in NSCLC cells and tissues. Both in vitro and in vivo experiments showed that circ-WHSC1 promoted NSCLC proliferation. circ-WHSC1 also promoted the migration and invasion of lung cancer cells. Through bioinformatic analysis and functional experiments, we showed that circ-WHSC1 could act as a sponge for micro-RNA-7 (miR-7) and regulate the expression of TAB2 (TGF-beta activated kinase one binding protein two). Inhibition of the circ-WHSC1/miR-7/TAB2 pathway could effectively attenuate lung cancer progression. In summary, this study confirmed the existence and oncogenic function of circ-WHSC1 in NSCLC. The research suggests that the circ-WHSC1/miR-7/TAB2 axis might be a potential target for NSCLC therapy.
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http://dx.doi.org/10.1111/jcmm.16925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505844PMC
October 2021

Comparison of the Variability of Small Extracellular Vesicles Derived from Human Liver Cancer Tissues and Cultured from the Tissue Explants Based on a Simple Enrichment Method.

Stem Cell Rev Rep 2021 Sep 22. Epub 2021 Sep 22.

The First School of Clinical Medicine, Gannan Medical University, Ganzhou, China.

A potential use of small extracellular vesicles (sEVs) for diagnostic and therapeutic purposes has recently generated a great interest. sEVs, when purified directly from various tissues with proper procedures, can reflect the physiological and pathological state of the organism. However, the quality of sEV is affected by many factors during isolation, including separation of sEV from cell and tissues debris, the use of enzymes for tissue digestion, and the storage state of tissues. In the present study, we established an assay for the isolation and purification of liver cancer tissues-derived sEVs (tdsEVs) and cultured explants-derived sEVs (cedsEVs) by comparing the quality of sEVs derived from different concentration of digestion enzyme and incubation time. The nano-flow cytometry (NanoFCM) showed that the isolated tdsEVs by our method are purer than those obtained from differential ultracentrifugation. Our study thus establishes a simple and effective approach for isolation of high-quality sEVs that can be used for analysis of their constituents.
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http://dx.doi.org/10.1007/s12015-021-10264-1DOI Listing
September 2021

Baicalin improves podocyte injury in rats with diabetic nephropathy by inhibiting PI3K/Akt/mTOR signaling pathway.

Open Med (Wars) 2021 2;16(1):1286-1298. Epub 2021 Sep 2.

Department of Neurology, Shenzhen Fuyong People's Hospital, Shenzhen, Guangdong 518103, China.

Objective: To investigate the effect of baicalin on diabetic nephropathy (DN) rats and podocytes and its mechanism.

Methods: The rat models with DN were established by high-fat and high-sugar diet and intraperitoneal injection of streptozotocin. The fasting blood glucose (FBG) and weight of rats in each group were measured at 0, 1, 2, 3, and 4 weeks. Their biochemical indicators, expression of inflammatory, and antioxidant factors were measured using an automatic biochemical analyzer together with ELISA. Hematoxylin-eosin staining and periodic acid-schiff staining were used to observe the morphological changes in the kidneys of rats in each group. Finally, the expressions of related molecules and PI3K/Akt/mTOR signaling pathway proteins in renal tissues and podocytes were examined by qRT-PCR and Western blot.

Results: Compared with the DN group, the FBG and weight, serum creatinine, blood urea nitrogen, total cholesterol, triacylglycerol, microalbumin, and albumin/creatinine ratio were all significantly decreased in the Baicalin treatment groups in a concentration-dependent manner. The levels of inflammatory factors in kidney tissue and podocytes were decreased. In addition, the activities of lactate dehydrogenase and malondialdehyde in tissue were decreased, while the superoxide dismutase was increased. The pathological sections showed that glomerular atrophy and glomerular basement membrane thickening caused by hyperglycemia were improved in the Baicalin treatment groups. Meanwhile, baicalin inhibited the downregulation of Nephrin and Podocin expressions and upregulation of Desmin expression caused by DN, and inhibited the expressions of p-PI3K, p-Akt, and p-mTOR proteins.

Conclusion: Baicalin slows down podocyte injury caused by DN by inhibiting the activity of PI3K/Akt/mTOR signaling pathway.
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http://dx.doi.org/10.1515/med-2021-0335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415539PMC
September 2021

Changes in the vasculature of human brain tumors: Implications for treatment.

Neuro Oncol 2021 Sep 13. Epub 2021 Sep 13.

Brain Barriers Research Center, Department of Pharmaceutics, College of Pharmacy, University of Minnesota, Minneapolis, Minnesota, USA.

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http://dx.doi.org/10.1093/neuonc/noab220DOI Listing
September 2021

Variation of bitter components of the asparagus juices during lactic acid bacteria fermentation.

Biosci Biotechnol Biochem 2021 Oct;85(11):2300-2310

Key Lab of Dairy Biotechnology and Safety Control, College of Food Science and Engineering, Yangzhou University, Jiangsu, China.

To investigate the bitterness status of asparagus juices during lactic acid fermentation, Limosilactobacillus fermentum Xd, Lacticaseibacillus paracasei Yd, Lactiplantibacillus plantarum 5-7-3, and their various combinations were used for single and mixed fermentation of asparagus juices. The fermentation characteristics and variation of the main bitter substances were studied. For the single and cofermented samples, the viable counts, pH value, and acidity were ranged from 8.33-8.65 lg CFU/mL, 3.58-3.86, and 6.29-6.52 g/kg, respectively. By sensory evaluation, the bitterness of every fermented sample was continuously reduced by at least 77% during fermentation, and the corresponding content of total saponins, flavonoids, and 9 bitter amino acids showed varying degrees of declination. These results suggested that it was feasible to develop novel low-bitter asparagus juices fermented by the lactic acid bacteria used in this study.
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http://dx.doi.org/10.1093/bbb/zbab158DOI Listing
October 2021

Co-treatment of copper smelting flue dust and arsenic sulfide residue by a pyrometallurgical approach for simultaneous removal and recovery of arsenic.

J Hazard Mater 2021 08 24;416:126149. Epub 2021 May 24.

State Key Laboratory of Advanced Metallurgy, University of Science and Technology Beijing, Beijing 100083, China; School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing 100083, China. Electronic address:

As the typical hazardous arsenic pollutants, copper smelting flue dust (CSFD) and arsenic sulfide residue (ASR) are produced extensively during copper smelting process, which pose significant pressure on environmental protection and green development of the copper industry. This work proposed an economic, efficient, and applicable approach to treat waste with waste, in which the simultaneous removal and recovery of As from CSFD and ASR were realized by a roasting process, with adding sulfuric acid, at a relatively low temperature (300-350 ℃). The thermodynamic analysis and experiments confirmed that the main phases of AsS and S in the ASR were used as a reductant for reducing As(Ⅴ) in the CSFD, and the introduction of sulfuric acid favorably enhanced the thermodynamic driving force and greatly lowered the reaction temperature. The results indicated that removal and behavior of As were highly dependent on the mass ratio of ASR to CSFD, roasting temperature, and HSO dosage. By regulating the parameters, the species AsS, AsO, and arsenate were all converted to volatile AsO, which could be captured and deposited in cold water. In the optimized co-treatment, a satisfied As removal efficiency of 96.12% was achieved, while getting the 97.03% pure AsO.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126149DOI Listing
August 2021

The Relationship Between Circulating Bone Morphogenetic Protein-4 and Inflammation Cytokines in Patients Undergoing Thoracic Surgery: A Prospective Randomized Study.

J Inflamm Res 2021 21;14:4069-4077. Epub 2021 Aug 21.

Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People's Republic of China.

Background: Bone morphogenetic protein-4 (BMP4) has been identified as an inflammation regulator in the diseases of arteries and other organs. However, the relationship between circulating BMP4 and perioperative inflammation remains unclear.

Patients And Methods: Forty patients undergoing lobectomy were randomly allocated into the Control group (not receiving flurbiprofen) and the Flurb group (received 100mg flurbiprofen during surgery). Arterial blood was obtained before surgery (T1), at the end of surgery (T2), and 24 hours after surgery (T3) to test the plasma concentrations of BMP4, its antagonist Noggin, interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), and IL-10. The relationship between BMP4 and other variables and the effects of flurbiprofen on BMP4 changes were investigated.

Results: A total of 35 patients were included. Circulating BMP4 was positively correlated with IL-1β (<0.01, r=0.575) and TNF-α (<0.01, r=0.491), negatively correlated with IL-10 (<0.01, r=-0.675), but not correlated with Noggin. The plasma concentrations of BMP4, IL-1β, and TNF-α increased at T2 (<0.01, compared with T1) and decreased at T3 (<0.05, compared with T2). BMP4 concentrations at T3 were significantly higher than at T1 in the Control group (<0.05), while showing no significant difference in the Flurb group. However, in the Flurb group, the relative changes of BMP4 and IL-1β at T2 and T3 were significantly lower than those in the Control group.

Conclusion: Circulating BMP4 was elevated during surgery and highly correlated with inflammation cytokines. The elevation of BMP4 and inflammatory cytokines could be alleviated by flurbiprofen, indicating that BMP4 may exert pro-inflammatory properties via cyclooxygenase-II signaling pathways.
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http://dx.doi.org/10.2147/JIR.S324775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387642PMC
August 2021

Preclinical Risk Evaluation of Normal Tissue Injury With Novel Radiosensitizers.

Int J Radiat Oncol Biol Phys 2021 Aug 14. Epub 2021 Aug 14.

Department of Radiation Oncology, Mayo Clinic, Rochester, Minnesota. Electronic address:

Genotoxic damage induced by radiation triggers a highly coordinated DNA damage response, and molecular inhibitors of key nodes within this complex response network can profoundly enhance the antitumor efficacy of radiation. This is especially true for drugs targeting the catalytic subunit of DNA-dependent protein kinase, which is a core component of the nonhomologous end-joining DNA repair pathway, and ataxia telangiectasia mutated, which coordinates cell cycle arrest, apoptosis, and DNA repair functionalities after radiation exposure. Unlike the more modest in vitro radiosensitizing effects seen with classic sensitizing agents such as cisplatin, 5-fluorouracil, or taxanes, DNA-dependent protein kinase or ataxia telangiectasia mutated inhibitors provide much more robust sensitizing effects in vitro, as might be anticipated from targeting these key DNA repair modulators. However, patients with homozygous inactivating mutations of ataxia telangiectasia mutated or mice with homozygous defects in DNA-dependent protein kinase (severe combined immunodeficiency) have profoundly enhanced acute normal tissue radiation reactions. Therefore, there is significant potential that the combination of small molecule inhibitors of these kinases with radiation could cause similar dose-limiting acute normal tissue toxicities. Similarly, although less understood, inhibition of these DNA repair response pathways could markedly increase the risk of late radiation toxicities. Because these potent radiosensitizers could be highly useful to improve local control of otherwise radiation-resistant tumors, understanding the potential for elevated risks of radiation injury is essential for optimizing therapeutic ratio and developing safe and informative clinical trials. In this review, we will discuss 2 straightforward models to assess the potential for enhanced mucosal toxicity in the oral cavity and small intestine established in our laboratories. We also will discuss similar strategies for evaluating potential drug-radiation interactions with regard to increased risks of debilitating late effects.
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http://dx.doi.org/10.1016/j.ijrobp.2021.08.003DOI Listing
August 2021

Long-term porosity and retreatability of oval-shaped canals obturated using two different methods with a novel tricalcium silicate sealer.

Clin Oral Investig 2021 Aug 14. Epub 2021 Aug 14.

Division of Endodontics, Department of Oral Biological & Medical Sciences, Faculty of Dentistry, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.

Objective: To evaluate the percentage volume of voids and gaps in oval-shaped canals obturated using two different methods with a tricalcium silicate-based sealer after short- or long-term storage. The long-term effect of storage on the efficiency of removing filling material was also investigated.

Materials And Methods: Forty premolar teeth with oval-shaped canals were instrumented to Reciproc R25 and obturated using single cone obturation (SCO) or warm vertical compaction (WVC) techniques with gutta-percha and HiFlow sealer. The specimens were stored at 100% humidity and 37°C for 2 weeks or 6 months and scanned using micro-computed tomography. Initial retreatment was performed up to a Reciproc R40, and the operating time was recorded. The residual material in the canal received a supplementary procedure using XP-endo Finisher R (XPFR) files. After each retreatment procedure, the specimens were rescanned.

Results: The percentage volume of voids and gaps in the SCO group was higher than that of the WVC group at both 2 weeks and 6 months (P < 0.05). The percentage volume of the filling material removed after initial retreatment and XPFR cleaning was significantly higher in the 6-month group than in the 2-week groups (P < 0.05). The proportion of the residual material decreased significantly when XPFR files were used, compared to the initial retreatment group (P < 0.05) in both storage times.

Conclusion: The efficiency of retreatment in the oval-shaped canal was closely related to the storage time rather than the filling technique using a tricalcium silicate sealer. The XPFR instrument proved effective in the removal of the remaining materials from the oval-shaped canal.

Clinical Relevance: Obturation of the oval-shaped canal with TSBS using the SCO technique in the coronal area needs to be optimized. The retreatment was less efficacious in freshly filled canals than aged filled canals.
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http://dx.doi.org/10.1007/s00784-021-04088-zDOI Listing
August 2021

Effect of intrathecal administration of exogenous noggin on neuropathic pain.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Jul;46(7):673-679

Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.

Objectives: To explore the effect of intrathecal administration of exogenous noggin (NOG) on the pain behavior in the neuropathic pain (NP) rats through L spinal nerve ligation (SNL), and to examine the regulative role of NOG in astrocyte activation, inflammatory cytokines and downstream signals.

Methods: A total of 40 adult male Sprague Dawley (SD) rats were randomly divided into 3 groups: a control group (=10), a SNL group (SNL+intrathecal injection of artificial cerebrospinal fluid, =15), and a SNL+NOG group (SNL+intrathecal injection of recombinant NOG protein, =15). Von-Frey filaments were used to test the changes of paw withdrawal threshold (PWT) at Day 1 before operation, and Day 1, Day 4, Day 7 and Day 14 after operation in each group. Immunofluorescence was used to observe the activation of astrocyte located in the dorsal horn of spinal cord in the 3 groups. Western blotting was conducted to detect the expression levels of glial fibrillary acidic protein (GFAP), interleukin-6 (IL-6), signal transducer and activator of transcription (STAT3) and phosphorylation STAT3 (p-STAT3).

Results: Compared with the control group, the PWT in the SNL group was markedly decreased at each time point, together with the increase in GFAP, IL-6 and the ratio of p-STAT3/STAT3 (all <0.05). Meanwhile, compared with the SNL group, the PWT in the lumbar swelling of spinal cord in the SNL+NOG group was elevated at Day 4 and lasted to Day 14 (<0.05), accompanied by the decrease in GFAP, IL-6 and the ratio of p-STAT3/STAT3 (all <0.05).

Conclusions: The intrathecal administration of NOG may alleviate NP in the SNL rats through inhibiting astrocyte activation and down-regulating the STAT3 signal pathway.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200715DOI Listing
July 2021

Time-concentration-dependent profile of histone modifications on human hepatocytes treated by trichloroacetic acid.

Int J Environ Health Res 2021 Aug 7:1-9. Epub 2021 Aug 7.

Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou, P.R. China.

Trichloroacetic acid (TCA) is a common non-volatile by-product of chlorination disinfection for drinking water. It is necessary to know the epigenetic toxicity and mechanisms for establishing safe exposure limit for environmental TCA exposure. This study explored the histone modification variations of TCA-treated human hepatocytes L-02 at different time and concentrations. TCA (0.1 mM, 0.3 mM and 0.9 mM) had an inhibitory effect on the growth of L-02 cells, with no significant changes in morphology. Treated with TCA for 24 h and 48 h, L-02 cells showed decreased mRNA and protein level of histone deacetylases (HDACs), but increased after 72 h. The downregulation of HDACs in early stage of TCA exposure might be one of the important reasons for the increase of H3K9ac level. These changes of histone modification may serve as early epigenetic biomarkers for TCA exposure and the related diseases, offering the safe environmental exposure concentration reference.
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http://dx.doi.org/10.1080/09603123.2021.1964448DOI Listing
August 2021

Association of the classification and severity of heart failure with the incidence of contrast-induced acute kidney injury.

Sci Rep 2021 07 28;11(1):15348. Epub 2021 Jul 28.

Department of Cardiology, Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, 3 East Qingchun Road, Hangzhou, Zhejiang Province, 310016, People's Republic of China.

Congestive heart failure (HF) is a known risk factor of contrast-induced acute kidney injury (CI-AKI). However, the relationship of the classification and severity of HF with CI-AKI remains under-explored. From January 2009 to April 2019, we recruited patients undergoing elective PCI who had complete pre- and post-operative creatinine data. According to the levels of ejection fraction (EF), HF was classified as HF with reduced EF (HFrEF) [EF < 40%], HF with mid-range EF (HFmrEF) [EF 40-49%] and HF with preserved EF (HFpEF) [EF ≥ 50%]. CI-AKI was defined as an increase of either 25% or 0.5 mg/dL (44.2 μmoI/L) in serum baseline creatinine level within 72 h following the administration of the contrast agent. A total of 3848 patients were included in the study; mean age 67 years old, 33.9% females, 48.1% with HF, and 16.9% with CI-AKI. In multivariate logistic regression analysis, HF was an independent risk factor for CI-AKI (OR 1.316, p value < 0.05). Among patients with HF, decreased levels of EF (OR 0.985, p value < 0.05) and elevated levels of N-terminal pro b-type natriuretic peptide (NT-proBNP) (OR 1.168, p value < 0.05) were risk factors for CI-AKI. These results were consistent in subgroup analysis. Patients with HFrEF were more likely to develop CI-AKI than those with HFmrEF or HFpEF (OR 0.852, p value = 0.031). Additionally, lower levels of EF were risk factors for CI-AKI in the HFrEF and HFmrEF groups, but not in the HFpEF group. NT-proBNP was an independent risk factor for CI-AKI in the HFrEF, HFmrEF and HFpEF groups. Elevated levels of NT-proBNP are independent risk factors for CI-AKI irrespective of the classification of HF. Lower levels of EF were risk factors for CI-AKI in the HFrEF and HFmrEF groups, but not in the HFpEF group.
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http://dx.doi.org/10.1038/s41598-021-94910-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319404PMC
July 2021

Novel Diethyl Ether Gas Sensor Based on Cataluminescence on Nano-Pd/ZnNiAlO.

ACS Omega 2021 Jul 29;6(27):17576-17583. Epub 2021 Jun 29.

Biochemical Engineering College, Beijing Union University, Beijing 100023, China.

A sensitive diethyl ether gas sensor based on cataluminescence on nano-Pd/ZnNiAlO at a temperature lower than 150 °C was reported. The composition of the sensitive material was determined by energy-dispersive spectrometry, and a particle size of less than 50 nm was shown by transmission electron microscopy. When the atomic percentage of Pd in the sensing material is 0.8-1.3%, it is beneficial to the low-temperature and high-selective cataluminescence of diethyl ether. The signal response and recovery of diethyl ether on the sensitive material can be completed quickly in 0.5 s, and the relative standard deviation of the signal within 500 h of continuous operation is not more than 2.5%. There is good linear relationship between the luminescence intensity and the concentration of diethyl ether in the range of 0.08-75 mg/m. The detection limit (3σ) is 0.04 mg/m. The working conditions optimized by the response surface methodology were an analytical wavelength of 548.86 nm, a reaction temperature of 109.18 °C, and a carrier gas velocity of 125.88 mL/min. The sensitivity of the method can be increased by 4.5% under the optimized working conditions. The optimization method is universal for many multi-parameter processes.
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http://dx.doi.org/10.1021/acsomega.1c02098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280636PMC
July 2021

Specific Borrmann classification in advanced gastric cancer by an ensemble multilayer perceptron network: a multicenter research.

Med Phys 2021 Sep 4;48(9):5017-5028. Epub 2021 Aug 4.

CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, China.

Purpose: Borrmann classification in advanced gastric cancer (AGC) is necessarily associated with personalized surgical strategy and prognosis. But few radiomics research studies have focused on specific Borrmann classification, and there is yet no consensus regarding what machine learning methods should be the most effective.

Methods: A combined size of 889 AGC patients was retrospectively enrolled from two centers. Radiomic features were extracted from tumors manually delineated on preoperative computed tomography images. Two classification experiments (Borrmann I/II/III vs. IV and Borrmann II vs. III) were conducted. In each task, we combined three common feature selection methods and five typical machine learning classifiers to construct 15 basic classification models, and then fed the 15 predictions to a designed multilayer perceptron (MLP) network.

Results: In internal and external validation cohorts, the proposed ensemble MLP yielded good performance with area under curves of 0.767 and 0.702 for Borrmann I/II/III vs. IV, as well as 0.768 and 0.731 for Borrmann II vs. III. Considering the imbalanced distribution of four Borrmann types (I, 2.9%; II, 12.8%; III, 69.5%; IV, 14.7%), the ensemble MLP surpassed the overfitting barrier and attained fine specificity (0.667 and 0.750 for Borrmann I/II/III vs. IV; 0.714 and 0.620 for Borrmann II vs. III) and sensitivity (0.795 and 0.610 for Borrmann I/II/III vs. IV; 0.652 and 0.703 for Borrmann II vs. III). Also, survival analysis showed that patients could be significantly risk stratified by MLP predicted types in both experiments (p < 0.0001, log-rank test).

Conclusions: This study proposed an MLP-based ensemble learning architecture, which could identify Borrmann type IV automatically and improve the differentiation of Borrmann type II from III. The study provided a new view for specific Borrmann classification in clinical practice.
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http://dx.doi.org/10.1002/mp.15094DOI Listing
September 2021

Effect of statins on post-contrast acute kidney injury: a multicenter retrospective observational study.

Lipids Health Dis 2021 Jul 5;20(1):63. Epub 2021 Jul 5.

Department of Cardiovascular Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, No 3 East of Qinchun Road, Hangzhou, Zhejiang, 310000, People's Republic of China.

Background: Post-contrast acute kidney injury (PC-AKI) is a severe complication of coronary angiography (CAG) and percutaneous coronary intervention (PCI). Currently, the effect of statins on PC-AKI and its mechanism remains unclear.

Methods: This multicenter retrospective observational study included 4386 patients who underwent CAG or PCI from December 2006 to December 2019 in Sir Run Run Shaw Hospital and its medical consortium hospitals. Serum creatinine pre- or post-procedure within 72 h after PCI was recorded. Multivariate logical regression was used to explore whether preoperative use of statins was protective from PC-AKI. The path analysis model was then utilized to look for the mediation factors of statins.

Results: Four thousand three hundred eighty-six patients were enrolled totally. The median age of the study population was 68 years old, 17.9% with PC-AKI, and 83.3% on preoperative statins therapy. The incidence of PC-AKI was significantly lower in group of patients on statins therapy. Multivariate regression indicated that preoperative statins therapy was significantly associated with lower percentage of elevated creatinine (β: -0.118, P < 0.001) and less PC-AKI (OR: 0.575, P < 0.001). In the preoperative statins therapy group, no statistically significant difference was detected between the atorvastatin and rosuvastatin groups (OR: 1.052, P = 0.558). Pathway model analysis indicated a direct protective effect of preoperative statins therapy on PC-AKI (P < 0.001), but not through its lipid-lowering effect (P = 0.277) nor anti-inflammatory effect (P = 0.596). Furthermore, it was found that "low-density lipoprotein cholesterol (LDL-C)→C-reactive protein (CRP)" mediated the relationship between preoperative statins therapy and PC-AKI (P = 0.007). However, this only explained less than 1% of the preoperative protective effects of statins on PC-AKI.

Conclusion: Preoperative statins therapy is an independent protective factor of PC-AKI, regardless of its type. This protective effect is not achieved by lipid-lowering effect or anti-inflammatory effect. These findings underscore the potential use of statins in preventing PC-AKI among those at risk.
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http://dx.doi.org/10.1186/s12944-021-01489-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258930PMC
July 2021

Optogenetic Dissection of Neural Circuits Underlying Stress-Induced Mood Disorders.

Front Psychol 2021 17;12:600999. Epub 2021 Jun 17.

Mental Health Education Center, Xidian University, Xi'an, China.

This review aims to (i) summarize the literature on optogenetic applications of different stress-induced mood disorder models of the medial prefrontal cortex (mPFC) and its projection circuits, and (ii) examine methodological variability across the literature and how such variations may influence the underlying circuits of stress-induced mood disorders. A variety of databases (PubMed, Web of Science, Elsevier, Springer, and Wiley) were systematically searched to identify optogenetic studies that applied to mood disorders in the context of stress. Eleven studies on optogenetic stimulation of the mPFC and the effect of its efferent circuitry on anxiety- and depression-like behaviors in different rodent models were selected. The results showed that the optogenetics (i) can provide insights into the underlying circuits of mood disorders in the context of stress (ii) and also points out new therapeutic strategies for treating mood disorders. These findings indicate a clear role for the mPFC in social avoidance, and highlight the central role of stress reactivity circuitry that may be targeted for the treatment of stress-induced mood disorders.
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http://dx.doi.org/10.3389/fpsyg.2021.600999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249197PMC
June 2021

mTORC1 is a mechanosensor that regulates surfactant function and lung compliance during ventilator-induced lung injury.

JCI Insight 2021 Jul 22;6(14). Epub 2021 Jul 22.

Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, and.

The acute respiratory distress syndrome (ARDS) is a highly lethal condition that impairs lung function and causes respiratory failure. Mechanical ventilation (MV) maintains gas exchange in patients with ARDS but exposes lung cells to physical forces that exacerbate injury. Our data demonstrate that mTOR complex 1 (mTORC1) is a mechanosensor in lung epithelial cells and that activation of this pathway during MV impairs lung function. We found that mTORC1 is activated in lung epithelial cells following volutrauma and atelectrauma in mice and humanized in vitro models of the lung microenvironment. mTORC1 is also activated in lung tissue of mechanically ventilated patients with ARDS. Deletion of Tsc2, a negative regulator of mTORC1, in epithelial cells impairs lung compliance during MV. Conversely, treatment with rapamycin at the time MV is initiated improves lung compliance without altering lung inflammation or barrier permeability. mTORC1 inhibition mitigates physiologic lung injury by preventing surfactant dysfunction during MV. Our data demonstrate that, in contrast to canonical mTORC1 activation under favorable growth conditions, activation of mTORC1 during MV exacerbates lung injury and inhibition of this pathway may be a novel therapeutic target to mitigate ventilator-induced lung injury during ARDS.
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http://dx.doi.org/10.1172/jci.insight.137708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8410036PMC
July 2021

The Hierarchical Structure and Predictive Validity of the Personality Inventory for in Chinese Nonclinical Adolescents.

Assessment 2021 Jun 17:10731911211022835. Epub 2021 Jun 17.

Beijing Normal University, Beijing, China.

To evaluate the factor structure, reliability, and validity of the Personality Inventory for (PID-5) in Chinese nonclinical adolescents, a total of 1,442 Chinese middle school youths ( = 14.85, girls = 52.4%) were recruited in the present study. All the participants completed the full-length 220-item PID-5. Some participants ( = 1,003) were administered adolescents' social adjustment as a criterion measure at the same time and 236 participants took part in longitudinal assessment of the PID-5 and adolescents' social adjustment 6 months later. First, exploratory structural equation modeling analyses supported a six-factor structure of the PID-5 in our present sample. Second, Negative Affectivity, Detachment, Antagonistic, and Disinhibition domains had positive correlations with negative social adjustment, and negative correlations with positive social adjustment concurrently and longitudinally, with the exception of Constraint and Psychoticism. Third, Cronbach's alpha for the PID-5 traits ranged from .57 to .91 in the full sample. The 6-month test-retest reliability by indexes of interclass correlation coefficient showed poor to good stability. As a whole, our findings provided preliminary evidence of the PID-5 as a reliable and valid measure of adolescents' maladaptive personality traits in mainland China.
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http://dx.doi.org/10.1177/10731911211022835DOI Listing
June 2021

Thermal chiral anomaly in the magnetic-field-induced ideal Weyl phase of BiSb.

Nat Mater 2021 Jun 7. Epub 2021 Jun 7.

Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH, USA.

The chiral anomaly is the predicted breakdown of chiral symmetry in a Weyl semimetal with monopoles of opposite chirality when an electric field is applied parallel to a magnetic field. It occurs because of charge pumping between monopoles of opposite chirality. Experimental observation of this fundamental effect is plagued by concerns about the current pathways. Here we demonstrate the thermal chiral anomaly, energy pumping between monopoles, in topological insulator bismuth-antimony alloys driven into an ideal Weyl semimetal state by a Zeeman field, with the chemical potential pinned at the Weyl points and in the absence of any trivial Fermi surface pockets. The experimental signature is a large enhancement of the thermal conductivity in an applied magnetic field parallel to the thermal gradient. This work demonstrates both pumping of energy and charge between the two Weyl points of opposite chirality and that they are related by the Wiedemann-Franz law.
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http://dx.doi.org/10.1038/s41563-021-00983-8DOI Listing
June 2021

Histone hypoacetylation contributes to neurotoxicity induced by chronic nickel exposure in vivo and in vitro.

Sci Total Environ 2021 Aug 10;783:147014. Epub 2021 Apr 10.

Department of Occupational Health, Army Medical University, 400038 Chongqing, People's Republic of China. Electronic address:

Nickel (Ni) is a heavy metal that is both an environmental pollutant and a threat to human health. However, the effects of Ni on the central nervous system in susceptible populations have not been well established. In the present study, the neurotoxicity of Ni and its underlying mechanism were investigated in vivo and in vitro. Ni exposure through drinking water (10 mg Ni/L, 12 weeks) caused learning and memory impairment in mice. Reduced dendrite complexity was observed in both Ni-exposed mouse hippocampi and Ni-treated (200 μM, 72 h) primary cultured hippocampal neurons. The levels of histone acetylation, especially at histone H3 lysine 9 (H3K9ac), were reduced in Ni-exposed mouse hippocampi and cultured neurons. RNA sequencing and chromatin immunoprecipitation (ChIP) sequencing analyses revealed that H3K9ac-modulated gene expression were downregulated. Treatment with sodium butyrate, a histone deacetylase inhibitor, attenuated Ni-induced H3K9 hypoacetylation, neural gene downregulation and dendrite complexity reduction in cultured neurons. Sodium butyrate also restored Ni-induced memory impairment in mice. These results indicate that Ni-induced H3K9 hypoacetylation may be a contributor to the neurotoxicity of Ni. The finding that Ni disturbs histone acetylation in the nervous system may provide new insight into the health risk of chronic Ni exposure.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147014DOI Listing
August 2021

Chicken Heat Shock Protein 70 Is an Essential Host Protein for Infectious Bursal Disease Virus Infection In Vitro.

Pathogens 2021 May 28;10(6). Epub 2021 May 28.

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.

Infectious bursal disease virus (IBDV) infection causes pathogenicity and mortality in chickens, leading to huge economic losses in the poultry industry worldwide. Studies of host-virus interaction can help us to better understand the viral pathogenicity. As a highly conservative host factor, heat shock protein 70 (Hsp70) is observed to be involved in numerous viral infections. However, there is little information about the role of chicken Hsp70 (cHsp70) in IBDV infection. In the present study, the increased expression of cHsp70 was observed during IBDV-infected DF-1 cells. Further studies revealed that Hsp70 had similar locations with the viral double-stranded RNA (dsRNA), and the result of pull-down assay showed the direct interaction between cHsp70 with dsRNA, viral proteins (vp)2 and 3, indicating that maybe cHsp70 participates in the formation of the replication and transcription complex. Furthermore, overexpression of cHsp70 promoted IBDV production and knockdown of cHsp70 using small interfering RNAs (siRNA) and reducedviral production, implying the necessity of cHsp70 in IBDV infection. These results reveal that cHsp70 is essential for IBDV infection in DF-1 cells, suggesting that targeting cHsp70 may be applied as an antiviral strategy.
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http://dx.doi.org/10.3390/pathogens10060664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229272PMC
May 2021

Insomnia symptoms are associated with metabolic syndrome in patients with severe psychiatric disorders.

Sleep Med 2021 07 19;83:168-174. Epub 2021 May 19.

Department of Sleep Medicine, Shantou University Mental Health Center, Shantou, Guangdong, China; Sleep Medicine Center, Shantou University Medical College, Shantou, Guangdong, China. Electronic address:

Objective: To examine the relationship between insomnia symptoms and metabolic syndrome in patients with severe psychiatric disorders.

Methods: We conducted a cross-sectional study including 272 inpatients (mean age: 34.06 ± 11.52 years, 67.3% males) with severe psychiatric disorders consecutively admitted in Shantou University Mental Health Center Inpatient Department. All patients underwent a psychiatric evaluation. Insomnia symptoms were assessed by the Pittsburgh Sleep Quality Index (PSQI) and defined present if PSQI>7. The diagnosis of metabolic syndrome was defined using the new International Diabetes Federation definition based on clinical and laboratory evaluation.

Results: Among the 272 patients, 94 (34.6%) presented insomnia symptoms. Overall, patients with insomnia symptoms had significantly higher percentage of metabolic syndrome (23.4% vs. 12.4%, p = 0.019) and hypertriglyceridemia (30.9% vs. 19.1%, p = 0.029), and marginally significantly higher levels of fasting insulin (58.75 ± 37.22 pmol/L vs. 51.72 ± 34.09 pmol/L, p = 0.050), homeostasis model assessment of insulin resistance (1.83 ± 1.31 vs. 1.62 ± 1.25, p = 0.055) and percentage of insulin resistance (55.3% vs. 44.4%, p = 0.086) compared to those without insomnia symptoms. Multiple logistic regressions showed that patients with insomnia symptoms had significantly higher odds for metabolic syndrome [odds ratio (OR) = 2.99, 95% confidence interval (CI) = 1.25-7.14], central obesity (OR = 3.02, 95% CI = 1.18-7.76), hypertriglyceridemia (OR = 2.46, 95% CI = 1.28-4.76) and marginally significantly higher odds for insulin resistance (OR = 1.68, 95% CI = 0.93-3.02) after controlling for potential confounders.

Conclusions: Within severely mentally ill patients, insomnia symptoms are associated with metabolic syndrome and insulin resistance. It appears that insomnia symptoms are independent clinical indicators of underlying metabolic syndrome in patients with severe psychiatric disorders.
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http://dx.doi.org/10.1016/j.sleep.2021.03.030DOI Listing
July 2021

Mutation of Gemin5 Causes Defective Hematopoietic Stem/Progenitor Cells Proliferation in Zebrafish Embryonic Hematopoiesis.

Front Cell Dev Biol 2021 30;9:670654. Epub 2021 Apr 30.

Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Fate determination and expansion of Hematopoietic Stem and Progenitor Cells (HSPCs) is tightly regulated on both transcriptional and post-transcriptional level. Although transcriptional regulation of HSPCs have achieved a lot of advances, its post-transcriptional regulation remains largely underexplored. The small size and high fecundity of zebrafish makes it extraordinarily suitable to explore novel genes playing key roles in definitive hematopoiesis by large-scale forward genetics screening. Here, we reported a novel zebrafish mutant line with a point mutation in gene obtained by ENU mutagenesis and genetic screening, causing an earlier stop codon next to the fifth WD repeat. Gemin5 is an RNA-binding protein with multifunction in post-transcriptional regulation, such as regulating the biogenesis of snRNPs, alternative splicing, stress response, and translation control. The mutants displayed specific deficiency in definitive hematopoiesis without obvious defects during primitive hematopoiesis. Further analysis showed the impaired definitive hematopoiesis was due to defective proliferation of HSPCs. Overall, our results indicate that Gemin5 performs an essential role in regulating HSPCs proliferation.
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http://dx.doi.org/10.3389/fcell.2021.670654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120239PMC
April 2021

No obvious association exists between mean platelet volume and hypertension subtypes.

Biomark Med 2021 06 14;15(8):577-584. Epub 2021 May 14.

Tianjin Key Laboratory of Lung Cancer Metastasis & Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, 300052, PR China.

To determine the association between mean platelet volume (MPV) and hypertension subtypes. 44,281 Chinese individuals were enrolled in this cross-sectional study. The mean blood pressure decreased with increasing MPV in females (p = 0.001) and increased MPV seemed to be a potential protective factor for isolated diastolic hypertension in models 1 and 2. The OR (CI) was 0.878 (0.789-0.976) for model 1 and 0.880 (0.789-0.981) for model 2 in males and 0.646 (0.495-0.841) for model 1 and 0.657 (0.503-0.858) for model 2 in females, when MPV was analyzed as a categorical variable. The OR (CI) was 0.947 (0.911-0.985) for Model 1 and 0.947 (0.910-0.985) for Model 2 in males, and 0.886 (0.807-0.973) for Model 1 and 0.892 (0.813-0.978) for Model 2 in females when MPV was analyzed as a continuous variable. However, the statistical difference of OR disappeared when we added blood-related covariates in Model 3. No obvious association exists between MPV and hypertension subtypes. Other blood parameters might have a greater impact on hypertension subtypes.
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http://dx.doi.org/10.2217/bmm-2020-0305DOI Listing
June 2021
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