Publications by authors named "Wenjing Cheng"

43 Publications

PirB functions as an intrinsic suppressor in hippocampal neural stem cells.

Aging (Albany NY) 2021 06 13;13(12):16062-16071. Epub 2021 Jun 13.

Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China.

Neural stem cells play pivotal roles during prenatal development and throughout life. Here, we report that Paired immunoglobulin-like receptor B (PirB) functions as a suppressor during brain neurogenesis in the adult mouse. PirB expression increased with age during development, and its deficiency promoted neural stem cell proliferation and differentiation and . Furthermore, we detected an increase in Type 1 neural stem cells in PirB-deficient mice compared to their wild-type littermates. PirB deficiency promoted stemness marker gene expression of Sox2 and KLF4 by activating Akt1 phosphorylation. These findings suggest that PirB inhibits the self-renewal and differentiation capacities of neural stem cells. Thus, PirB may have the potential to serve as a therapeutic target for treatment of reduced neurogenesis in adults due to aging or other pathological conditions.
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http://dx.doi.org/10.18632/aging.203134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266311PMC
June 2021

Acupuncture Can Play an Antidepressant Role by Regulating the Intestinal Microbes and Neurotransmitters in a Rat Model of Depression.

Med Sci Monit 2021 May 27;27:e929027. Epub 2021 May 27.

Department of Traditional Chinese Medicine, School of Medicine, Xiamen University, Xiamen, Fujian, China (mainland).

BACKGROUND Acupuncture, which has many good effects and few adverse effects, is widely recognized as an alternative therapy for depression in clinical practice. This study aimed to explore the mechanism of acupuncture in antidepressant treatment. MATERIAL AND METHODS In this experiment, Sprague-Dawley rats were randomly divided into 4 groups: control, chronic unpredictable mild stress (CUMS), acupuncture, and fluoxetine groups. The CUMS, acupuncture, and fluoxetine groups were orphaned and subjected to chronic unpredictable stress for 6 weeks, and the acupuncture and fluoxetine groups were treated with their respective intervention in weeks 4-6. The body weight of rats was monitored weekly. After behavioral tests were completed, serum, feces, and hippocampal tissue of rats were collected. RESULTS The results showed that the acupuncture and fluoxetine treatments could alleviate the behavioral changes caused by CUMS. The treatments increased the total distance of rat crossing in the open-field test, prolonged the activity time of the open cross maze in the open arm, and improved the rate of sucrose consumption in the sucrose preference test. In addition, both the decreased level of dopamine (DA) and 5-hydroxytryptamine (5-HT) in serum and hippocampus caused by CUMS were improved after the treatments with acupuncture and fluoxetine, and the decreased expression of brain-derived neurotrophic factor signaling and the astrocytes in the hippocampus caused by CUMS were increased after the treatments with acupuncture and fluoxetine. Acupuncture and fluoxetine also decreased the ß isoform of calmodulin-dependent protein kinase II in the hippocampus, which was increased by CUMS. Furthermore, acupuncture regulated intestinal microbial disorders caused by CUMS, which reduced the relative abundance ratio of Bacteroidetes/Firmicutes in rats. CONCLUSIONS Our experimental results indicate that acupuncture can alleviate depression-like performance in CUMS rats by regulating intestinal microbes and neurotransmitters.
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http://dx.doi.org/10.12659/MSM.929027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168287PMC
May 2021

miRNA-467b inhibits Th17 differentiation by targeting eIF4E in experimental autoimmune encephalomyelitis.

Mol Immunol 2021 05 20;133:23-33. Epub 2021 Feb 20.

Department of Immunology and Microbiology, Shanghai JiaoTong University School of Medicine, Shanghai Institute of Immunology, Shanghai 200025, China. Electronic address:

Multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE) are neuroinflammatory autoimmune diseases characterized by the axonal loss, demyelination, and neurodegeneration of the central nervous system. Overactivation of CD4 T cells, especially the migration of the Th1 and Th17 subsets into the central nervous system (CNS), leads to the secretion of inflammatory mediators and destruction of the contact between neurons and activated macrophages, which can then result in a series of neurocognitive and motor deficits. In this study, we intended to explore the role of miRNA-467b in regulating Th cell development in EAE. We found that the level of miRNA-467b was decreased and eukaryotic initiation factor 4 F (eIF4E) was increased in lymph nodes and the CNS at EAE peak. eIF4E was confirmed as the direct target of miRNA467b. Overexpression of miRNA-467b could suppress a percentage of CD4 IL-17 cells in EAE CD4 + T cells in vitro. In addition, we also identified miRNA-467b, which could suppress Th17 cell differentiation by targeting eIF4E in vitro. Furthermore, injecting miRNA-467b mimics into the caudal vein of EAE mice contributed to less inflammation in the peripheral lymphoid organs and CNS and alleviated disease severity. Taken together, our findings imply that miRNA-467b inhibits the differentiation and function of Th17 cells by targeting eIF4E, thereby alleviating EAE.
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http://dx.doi.org/10.1016/j.molimm.2021.02.008DOI Listing
May 2021

Paraventricular Nucleus P2X7 Receptors Aggravate Acute Myocardial Infarction Injury via ROS-Induced Vasopressin-V1b Activation in Rats.

Neurosci Bull 2021 May 23;37(5):641-656. Epub 2021 Feb 23.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

The present study was designed to investigate the mechanisms by which P2X7 receptors (P2X7Rs) mediate the activation of vasopressinergic neurons thereby increasing sympathetic hyperactivity in the paraventricular nucleus (PVN) of the hypothalamus of rats with acute myocardial ischemia (AMI). The left anterior descending branch of the coronary artery was ligated to induce AMI in rats. The rats were pretreated with BBG (brilliant blue G, a P2X7R antagonist), nelivaptan (a vasopressin V1b receptor antagonist), or diphenyleneiodonium (DPI) [an nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor]. Hemodynamic parameters of the heart were monitored. Myocardial injury and cardiomyocyte apoptosis were assessed. In the PVN of AMI rats, P2X7R mediated microglial activation, while reactive oxygen species (ROS) and NADPH oxidase 2 (NOX2) were higher than in the sham group. Intraperitoneal injection of BBG effectively reduced ROS production and vasopressin expression in the PVN of AMI rats. Moreover, both BBG and DPI pretreatment effectively reduced sympathetic hyperactivity and ameliorated AMI injury, as represented by reduced inflammation and apoptosis of cardiomyocytes. Furthermore, microinjection of nelivaptan into the PVN improved cardiac function and reduced the norepinephrine (AE) levels in AMI rats. Collectively, the results suggest that, within the PVN of AMI rats, P2X7R upregulation mediates microglial activation and the overproduction of ROS, which in turn activates vasopressinergic neuron-V1b receptors and sympathetic hyperactivity, hence aggravating myocardial injury in the AMI setting.
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http://dx.doi.org/10.1007/s12264-021-00641-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099953PMC
May 2021

Acid-etched Fe/FeO nanoparticles encapsulated into carbon cloth as a novel voltammetric sensor for the simultaneous detection of Cd and Pb.

Analyst 2021 Jan 19;146(2):691-697. Epub 2020 Nov 19.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, National Demonstration Center for Experimental Chemistry Education, College of Chemistry & Material Science, Northwest University, Xi'an 710127, China.

A portable electrode with usability, availability, and high-sensitivity is of great significance for effective on-site detection in practical situations. In this paper, a novel flexible, disposable sensor for Cd and Pb with ultrahigh sensitivity and a fast response, based on acid-etched Fe/FeO encapsulated into a disposable carbon cloth electrode, has been successfully fabricated. Differential pulse anode stripping voltammetry (DPASV) was used to investigate the stripping behavior of Cd and Pb, achieving high sensitivity for Cd and Pb (338.7 and 408.0 μA mM cm) with limits of detection (LODs) of 0.42 ppb and 0.50 ppb, respectively. Meanwhile, remarkable stability and reproducibility were obtained. Such an electrode can detect Cd and Pb in actual water samples so this is a good candidate to act as a simple and convenient sensor for general applications. More importantly, the novel disposable electrode exhibited the unique advantages of convenience, portability, and reliability compared to a conventional electrode, which may make it an alternative advantageous choice for practical on-site detection.
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http://dx.doi.org/10.1039/d0an01861aDOI Listing
January 2021

Portable electrochemical carbon cloth analysis device for differential pulse anodic stripping voltammetry determination of Pb.

Mikrochim Acta 2020 Oct 17;187(11):613. Epub 2020 Oct 17.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, National Demonstration Center for Experimental Chemistry Education, College of Chemistry and Material Science, Northwest University, Xi'an, 710127, China.

A novel electrochemical carbon cloth (CC) analysis device (eCAD) is proposed for the determination of Pb in environmental water samples, which was assembled using a single-step functional CC as both the sensing and the substrate material. The modified CC was characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, Raman spectra, and electrochemical impedance spectroscopy. The increase in electrochemical activity is due to the increased defective extent and excellent electrochemical activity of CC. Under optimum conditions (viz. a pH value of 4.5, deposition time of 160 s), the sensor is capable of determining Pb by differential pulse anodic stripping voltammetry (DPASV) at a typical working potential of - 1.0 V (vs. Ag/AgCl). Response is linear from 5.0 × 10 to 3.0 × 10 M Pb, and the detection limit is 4.8 nM (at S/N = 3). The sensor was successfully applied to the determination of Pb in real samples, with apparent recoveries from 96.0 to 102.0% and a relative standard deviation of less than 3.4%. In addition, the integration of the sensor with signal collection components has enabled us to realize on-site analysis of Pb, which is highlighted as a new generation of electrode platform for the development of a portable analysis device.Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04549-4DOI Listing
October 2020

infection in China: a retrospective report of 28 cases.

Emerg Microbes Infect 2020 Dec;9(1):2348-2357

Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xian, People's Republic of China.

infection is a rare and fatal disease. We have recorded 28 cases of infection during the past 20 years. Eighteen patients (64%) were male and 10 (36%) were female. Patient age ranged from 3 to 74 (mean, 27) years. Patient locations were distributed among 12 Provinces in China. Twenty-seven (96%) patients lived in rural areas, and 17 (61%) patients reported a history of trauma before the appearance of skin lesions. All cases presented with skin lesions as the primary symptom, and 16 (57%) cases developed encephalitis. Histopathology of skin lesions revealed granulomatous changes with histiocytes, lymphocytes, and plasma cells infiltration. Amebas were identified in all cases with immunohistochemical staining. Follow-up information was available in 27 (96%) cases. Fifteen (56%) patients died due to encephalitis and 12 (44%) were free of disease after treatment. Our results show that the clinical characteristics of infection in China are very different from those in the US. Infection of traumatized skin may play an important role in the pathogenesis of the disease in China. Encephalitis usually develops 3-4 years after skin lesions in Chinese cases. Patients with only skin lesions have a higher cure rate than patients with encephalitis.
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http://dx.doi.org/10.1080/22221751.2020.1835447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599003PMC
December 2020

Formation and characteristics of filamentous granular sludge.

Water Sci Technol 2020 Jul;82(2):364-372

School of Ecology and Environment, Zhengzhou University, Zhengzhou, 100 Science Avenue, 450001, China E-mail:

Aerobic granular sludge process as a promising biotechnology has been one of the research hotspots in the area of wastewater treatment during the last two decades. In our study, after around 60 days' operation, filamentous granular sludge (FGS) was formed under low aeration (SAV = 0.085 cm/s) and multi-feeding conditions. The characteristics of FGS and the performance of the FGS system for organic matter and nutrients removal were investigated. The results showed that chemical oxygen demand (COD) and total organic carbon (TOC) removal efficiencies were relatively stable, while COD removal efficiency increased from 82% to 94% in the presence of sulfamethoxazole (SMZ) at low concentration (1 mg/L). At the same time, the TP removal efficiency could be improved and maintained at around 75%, while TN removal efficiency was flocculated at around 50%. The analysis of microbial diversity showed that Thiothrix and Trichococcus as typical filamentous species were detected and dominant in the FGS system. The abundance of Thiothrix increased from 15% to 34%, while Trichococcus decreased from 23% to 3% in the presence of SMZ.
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http://dx.doi.org/10.2166/wst.2020.278DOI Listing
July 2020

Macroscopic macroporous titanosilicate constructed of a micro-mesoporous ultrathin nanofilm.

Chem Commun (Camb) 2020 Mar;56(20):3027-3030

State Key Laboratory Base of Novel Functional Materials and Preparation Science, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.

A novel micro-mesoporous nanofilm-constructed macroscopic macroporous titanosilicate (MNCMM-TiSi) has been successfully prepared by a templating approach. The resultant materials exhibit greatly improved TOFs in bulky alkene epoxidation compared to the conventional TS-1 zeolite and Ti-MCM-41.
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http://dx.doi.org/10.1039/c9cc09556jDOI Listing
March 2020

SlCAND1, encoding cullin-associated Nedd8-dissociated protein 1, regulates plant height, flowering time, seed germination, and root architecture in tomato.

Plant Mol Biol 2020 Mar 8;102(4-5):537-551. Epub 2020 Jan 8.

Bioengineering College, Chongqing University, Chongqing, 400044, China.

Key Message: Silencing of SlCAND1 expression resulted in dwarfish, loss of apical dominance, early flowering, suppression of seed germination, and abnormal root architecture in tomato Cullin-RING E3 ligases (CRLs)-dependent ubiquitin proteasome system mediates degradation of numerous proteins that controls a wide range of developmental and physiological processes in eukaryotes. Cullin-associated Nedd8-dissociated protein 1 (CAND1) acts as an exchange factor allowing substrate recognition part exchange and plays a vital role in reactivating CRLs. The present study reports on the identification of SlCAND1, the only one CAND gene in tomato. SlCAND1 expression is ubiquitous and positively regulated by multiple plant hormones. Silencing of SlCAND1 expression using RNAi strategy resulted in a pleiotropic and gibberellin/auxin-associated phenotypes, including dwarf plant with reduced internode length, loss of apical dominance, early flowering, low seed germination percentage, delayed seed germination speed, short primary root, and increased lateral root proliferation and elongation. Moreover, application of exogenous GA or IAA could partly rescue some SlCAND1-silenced phenotypes, and the expression levels of gibberellin/auxin-related genes were altered in SlCAND1-RNAi lines. These facts revealed that SlCAND1 is required for gibberellin/auxin-associated regulatory network in tomato. Although SlCAND1 is crucial for multiple developmental processes during vegetative growth stage, SlCAND1-RNAi lines didn't exhibit visible effect on fruit development and ripening. Meanwhile, we discussed that multiple physiological functions of SlCAND1 in tomato are different to previous report of its ortholog in Arabidopsis. Our study adds a new perspective on the functional roles of CAND1 in plants, and strongly supports the hypothesis that CAND1 and its regulated ubiquitin proteasome system are pivotal for plant vegetative growth but possibly have different roles in diverse plant species.
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http://dx.doi.org/10.1007/s11103-020-00963-7DOI Listing
March 2020

A pH-Induced Reversible Assembly System with Resveratrol-Controllable Loading and Release for Enhanced Tumor-Targeting Chemotherapy.

Nanoscale Res Lett 2019 Sep 6;14(1):305. Epub 2019 Sep 6.

Department of Hematopathology, Jiaozuo People's Hospital, Jiaozuo, Henan, 454000, China.

In this report, we present a pH-induced reversible assembly system (PIRAS) based on ferritin (Ft) for targeted tumor therapy. It has been developed to easily load and release of the anticancer drug resveratrol (RV), based on its natural pH-sensitivity and unique hollow cavity of Ft. A tumor-specific target peptide Arg-Gly-Asp (RGD) was conjugated onto the surface of RV-loaded Ft ([email protected]), to form biocompatible nanoparticles ([email protected]). The pH-sensitivity of Ft allows it to be denatured into a hollow porous nanosphere under acidic condition and renatured into a sealed hollow nanosphere under neutral condition. Using pH manipulation, [email protected], with a ~ 21 nm diameter, showed a high RV loading ratio of 79.6%. pH-triggered RV release was then measured at a ratio of 50.3% at pH5.0 over 24 h. Under neutral condition, the [email protected] showed excellent stability over 20 days. Confocal fluorescence imaging showed that [email protected] had a high cell uptake ratio and co-localization with the lysosome, mainly due to the RGD-mediated target effect. Based on the high drug loading, pH-triggered release, and tumor cell targeting effect, [email protected] showed great cell-killing ability in vitro and in vivo. The biocompatibility in vitro and in vivo was also demonstrated to be excellent, without systematic toxicity. The design concept of PIRAS based on Ft significantly inhibits tumor growth and simultaneously further broadens the application of Ft in nanomedicine.
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http://dx.doi.org/10.1186/s11671-019-3139-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6730982PMC
September 2019

AIE Nanoassemblies for Discrimination of Glycosaminoglycans and Heparin Quality Control.

Anal Chem 2019 08 10;91(15):10295-10301. Epub 2019 Jul 10.

Jiangsu Key Laboratory of Pesticide Science, Department of Chemistry, College of Sciences , Nanjing Agricultural University , Nanjing , Jiangsu 210095 , People's Republic of China.

The discrimination of glycosaminoglycans (GAGs) is a challenging task but of great importance to ensure their safe use in clinics. Herein, four supramolecular AIE nanoassemblies denoted as , , , and were synthesized by loading different amounts of the negatively charged AIEgen onto the surface of the positively charged polymer . These AIE nanoassemblies were utilized for the construction of a fluorescent sensor array, which was able to discriminate various GAGs on the basis of a compaction to displacement reaction mechanism. LDA and HCA results revealed that GAGs including Hep, Chs, HA, CTS, DS, and OSCS can be discriminated with 100% accuracy. The four-sensor array was then simplified to a three-sensor array using , , and as the sensors, which was determined to be still powerful in discrimination of various GAGs, accurate identification of unknown GAG samples, sensitive detection of trace OSCS contaminant in Hep, and identification of Hep and other biologically abundant anions. Moreover, the three-sensor array was even successfully applied for differentiating GAGs in serum media.
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http://dx.doi.org/10.1021/acs.analchem.9b02516DOI Listing
August 2019

Infiltrating T helper 17 cells in the paraventricular nucleus are pathogenic for stress-induced hypertension.

Biochem Biophys Res Commun 2019 07 24;515(1):169-175. Epub 2019 May 24.

School of Life Science, Shanghai University, Shanghai, 200444, China. Electronic address:

Central neuroinflammation produced by both innate and adaptive immunities plays a major role in the development of stress-induced hypertension (SIH), but successful T cell immunoregulation for SIH requires that the T cells can access brain tissues. So far, both the effects of T helper 17 (Th17) cells on SIH and the pathway for T cells entry into the brain were unknown. Here we show that the blood pressure (BP), heart rate (HR) and the norepinephrine(NE) of the SIH rats were considerably higher, the numbers of Th17 cells and IL-17 were higher, relative to control. Anti-IL-17 attenuated the elevation of BP and HR of the SIH rats when microinjected into the paraventricular nucleus (PVN).Alb-FITC, after infusion into the carotid artery, were found in the brain parenchyma of the PVN in the SIH rats. We concluded that Th17 cells infiltrated the parenchyma of the paraventricular nucleus (PVN) via a compromised blood brain barrier (BBB) in response to stress and Th17 cells and IL-17 play an important role in the pathophysiology of SIH.
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http://dx.doi.org/10.1016/j.bbrc.2019.05.121DOI Listing
July 2019

General Strategy for in Situ Generation of a Coumarin-Cu Complex for Fluorescent Water Sensing.

Anal Chem 2019 05 12;91(9):5817-5823. Epub 2019 Apr 12.

Jiangsu Key Laboratory of Pesticide Science, Department of Chemistry, College of Sciences , Nanjing Agricultural University , Nanjing , Jiangsu 210095 , China.

The detection of moisture in organic solvents is very important before their use in water-sensitive reactions. Herein, we report that coumarins D1 and D2 were able to generate the corresponding water-sensitive copper complexes D1-Cu and D2-Cu in common organic solvents, which can be used as efficient fluorescent turn-on sensors for water. Single-crystal diffraction analysis of the reaction product indicated that the sensing mechanism is based on the formation of a water-bridged 3D supramolecular hydrogen-bonding network. We demonstrated that a hydroxyl or amine group substituted at the 7-position of the coumarin framework played a key role in the water-sensing performance of D1-Cu and D2-Cu, by acting as a hydrogen bond acceptor in the supramolecular network. This provided a general strategy for designing such coumarin-based copper complexes for fluorescent water sensing. The water-sensing behavior of D1-Cu and D2-Cu were determined to be fast, pH tolerant, and sensitive. As low as 0.0525 wt % of water in methanol can be detected using D1-Cu as the sensor. Moreover, D1-Cu was successfully used for moisture sensing in real commercial products.
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http://dx.doi.org/10.1021/acs.analchem.9b00132DOI Listing
May 2019

Solvent directed selective and sensitive fluorescence detection of target ions using a coumarin-pyridine probe.

Analyst 2018 Nov;143(22):5583-5588

Jiangsu Key Laboratory of Pesticide Science, Department of Chemistry, College of Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.

The development of fluorescent probes to selectively and sensitively detect more than one target analyte under a given condition is a challenging task due to the lack of designing strategies. In this paper, we report the utilisation of the solvatochromic properties of a coumarin-pyridine based probe L1 to detect Mg2+ and PPi, respectively, by using different solvents such as acetonitrile, DMF and DMF-HEPES buffered solutions. When acetonitrile was used as the solvent, L1 was weakly fluorescent and showed a selective and sensitive fluorescence "turn on" response towards Mg2+ with a detection limit of 86 nM, while in DMF and DMF-HEPES buffered solutions, L1 was brightly fluorescent and was able to form a non-fluorescent L1-Fe3+ complex. The obtained L1-Fe3+ complex was further determined to be a selective and sensitive fluorescent "turn on" probe for PPi with a detection limit of 94 nM.
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http://dx.doi.org/10.1039/c8an01736kDOI Listing
November 2018

Asiatic acid protects differentiated PC12 cells from Aβ-induced apoptosis and tau hyperphosphorylation via regulating PI3K/Akt/GSK-3β signaling.

Life Sci 2018 Sep 11;208:96-101. Epub 2018 Jul 11.

Department of Neurology, No.1 People's Hospital of Jining City, Jining, Shandong, China. Electronic address:

Amyloid β (Aβ) peptide can cause neurotoxicity in Alzheimer's disease (AD). The main purpose of the present study is to investigate the protective role of asiatic acid (AA) against Aβ-induced neurotoxicity in neuronally differentiated PC12 cells. Differentiated PC12 cells were pretreated with 5, 10 or 20 μM AA before treatment with 20 μM Aβ. The viability and apoptosis of differentiated PC12 cells were determined by MTT assay and Annexin V-FITC/PI double staining, respectively. The mitochondrial membrane potential (MMP) of differentiated PC12 cells was analyzed by JC-1 staining. The expression levels of proteins were detected by western blot analysis. We found that AA significantly increased the viability of differentiated PC12 cells but attenuated the mitochondria-mediated apoptosis dose-dependently when challenging with Aβ. Besides, the results of western blot analysis showed that AA prevented IκBα degradation and p65 nuclear translocation, and promoted the phosphorylation of Akt and GSK-3β in Aβ-treated differentiated PC12 cells. Moreover, LY294002, a specific PI3K inhibitor, was found to abolish the beneficial effects of AA on Aβ-induced apoptosis and tau protein hyperphosphorylation. Our findings demonstrated that AA protects differentiated PC12 cells from Aβ-induced apoptosis and tau protein hyperphosphorylation, which might be partially mediated by the activation of the PI3K/Akt/GSK-3β signaling pathway.
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http://dx.doi.org/10.1016/j.lfs.2018.07.016DOI Listing
September 2018

Tomato (Solanum lycopersicum) SlIPT4, encoding an isopentenyltransferase, is involved in leaf senescence and lycopene biosynthesis during fruit ripening.

BMC Plant Biol 2018 Jun 5;18(1):107. Epub 2018 Jun 5.

Bioengineering College, Chongqing University, Chongqing, 400044, China.

Background: Lycopene is an important carotenoid pigment in red fruits and vegetables, especially in tomato. Although lycopene biosynthesis and catabolism have been found to be regulated by multiple factors including phytohormones, little is known about their regulatory mechanism. Cytokinins are crucial to various aspects of plant growth. Isopentenyltransferases (IPTs) catalyze the initial rate-limiting step of cytokinins biosynthesis, however, their roles in fruit ripening remain unclear.

Results: Here, the functions of SlIPT4, encoding an isopentenyltransferase, were characterized via RNAi-mediated gene silencing in tomato. As we expected, silencing of SlIPT4 expression resulted in accelerated leaf senescence. However, down-expression of SlIPT4 generated never-red orange fruits, corresponding with a dramatic reduction of lycopene. Among lycopene biosynthesis-related genes, the fact of remarkable decrease of ZISO transcript and upregulation of other genes, revealed that SlIPT4 regulates positively lycopene biosynthesis via directly affecting ZISO expression, and also supported the existence of regulatory loops in lycopene biosynthesis pathway. Meanwhile, the accumulation of abscisic acid (ABA) was reduced and the transcripts PSY1 were increased in SlIPT4-RNAi fruits, supporting the feedback regulation between ABA and lycopene biosynthesis.

Conclusion: The study revealed the crucial roles of SlIPT4 in leaf senescence and the regulatory network of lycopene biosynthesis in tomato, providing a new light on the lycopene biosynthesis and fruit ripening.
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http://dx.doi.org/10.1186/s12870-018-1327-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987576PMC
June 2018

Overexpression of an EIN3-binding F-box protein2-like gene caused elongated fruit shape and delayed fruit development and ripening in tomato.

Plant Sci 2018 Jul 20;272:131-141. Epub 2018 Apr 20.

Bioengineering College, Chongqing University, Chongqing 400044, China. Electronic address:

Ethylene signaling converges on the ETHYLENE-INSENSITIVE3 (EIN3)/EIN3-like (EIL) transcription factors to regulate a wide range of developmental processes in plants. EBF1/2 (EIN3-binding F-box protein 1 and 2) negatively regulate the ethylene signaling pathway by mediating the degradation of EIN3/EIL proteins. We uncovered previously that SlEBF1 and SlEBF2 are involved in ethylene response, plant senescence, and fruit ripening in tomato. The present study reports on the identification of a novel tomato F-box gene, designated as SlEBF2-like due that its encoded protein is greater similarity to SlEBF2. The SlEBF2-like promoter region contains three ethylene-response elements (EREs). SlEBF2-like is upregulated by ethylene and downregulated by ethylene inhibitors in tomato seedlings. It is dynamically expressed in flowers during bud-to-anthesis and anthesis-to-post-anthesis transitions, and at the onset of fruit ripening, suggesting its role in these situations where ethylene is required for flower opening and fruit ripening. SlEBF2-like overexpression leaded to fruit elongation, caused ripening and color change to start from fruit bottom and expand gradually to the pedicel, and strongly delayed fruit development and ripening in tomato. Our study indicates that the novel EBF gene, SlEBF2-like, is involved in fruit development and ripening via regulating the ethylene response in tomato.
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http://dx.doi.org/10.1016/j.plantsci.2018.04.016DOI Listing
July 2018

T-614 Promotes Osteoblastic Cell Differentiation by Increasing Dlx5 Expression and Regulating the Activation of p38 and NF-B.

Biomed Res Int 2018 19;2018:4901591. Epub 2018 Feb 19.

Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Immunology, 280 South Chongqing Road, Shanghai 200025, China.

Rheumatoid arthritis (RA) is an autoimmune inflammatory disease characterized by bone loss. Degree of inflammation has been identified as an important initiator of skeletal damage in RA. Iguratimod (T-614) is an anti-inflammatory agent which has been reported to show the inhibitory effect of bone destruction in RA. However, the role of T-614 in osteoblast differentiation is still not clear. In this study, we intended to find the effect of T-614 on the osteogenesis process. We detected osteogenesis markers and transcription factors associated with osteoblastic lineage and bone formation in the culture of mesenchymal stem cells which differentiate osteoblast. The contents and activity of alkaline phosphatase, levels of collagen type I and bone gla protein, and calcium nodule formation were increased significantly after T-614 treated. Meanwhile, the mRNAs expressions of and were also found to be increased significantly by real-time PCR. The changes of levels of phosphorylation of p38 and NF-B were also detected by Western blot. The results showed that T-614 promotes osteoblastic differentiation by increasing the expression of and and increasing the activation of P38. T-614 could advance the ectopic expression of NF-B to suppress inflammation, which indirectly inhibits the damage of the osteoblasts.
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http://dx.doi.org/10.1155/2018/4901591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5836304PMC
September 2018

IFN-β regulates Th17 differentiation partly through the inhibition of osteopontin in experimental autoimmune encephalomyelitis.

Mol Immunol 2018 01 8;93:20-30. Epub 2017 Nov 8.

Department of Immunology and Microbiology, Shanghai JiaoTong University School of Medicine, Shanghai Institute of Immunology, Shanghai 200025, China. Electronic address:

Multiple sclerosis (MS) and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), are chronic neuroinflammatory autoimmune diseases. Increased activation of CD4+T cells, especially the Th1 and Th17 subsets, is thought to play a causal role in this disease. IFN-β is widely used in the treatment of MS and is found to decrease IL-17 and OPN production in MS patients and EAE mice. However, a definitive molecular mechanism has not yet been fully elucidated. In this study, we investigated the immunomodulatory effect of IFN-β on the EAE model. We observed disease progression and determined the percentage of Th1/Th17 cells in the peripheral immune organs, brain, and spinal cord of mice. Furthermore, the levels of related cytokines and transcription factors were measured in splenocytes, and the effects of IFN-β on Th17 differentiation were assessed in vitro. Compared to the control group, IFN-β treatment significantly reduced the incidence of EAE and the associated pathological damage. Th1 and Th17 cells in IFN-β-treated mice were significantly reduced, and the levels of cytokines, such as IFN-γ, IL-17, and OPN, were significantly decreased in splenocyte supernatants as well as the levels of corresponding transcription factors. IFN-β inhibited downstream inflammatory cytokines through the inhibition of PI3K/AKT/NF-κB axis and p38, JNK-MAPK, as well as the regulation of mTOR complexes. Moreover, IFN-β inhibited Th17 differentiation and neutralizing OPN antibodies offset the inhibitory effect of IFN-β on Th17 cells. Meanwhile, IFN-β influenced the acetylation of the Il17a and Opn gene promoters. The findings described herein provide novel evidence for the role of IFN-β in Th17 differentiation partly through the inhibition of OPN.
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http://dx.doi.org/10.1016/j.molimm.2017.11.002DOI Listing
January 2018

Neuroinflammation contributes to autophagy flux blockage in the neurons of rostral ventrolateral medulla in stress-induced hypertension rats.

J Neuroinflammation 2017 Aug 23;14(1):169. Epub 2017 Aug 23.

Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai, 200032, People's Republic of China.

Background: Neuroinflammation plays hypertensive roles in the uninjured autonomic nuclei of the central nervous system, while its mechanisms remain unclear. The present study is to investigate the effect of neuroinflammation on autophagy in the neurons of the rostral ventrolateral medulla (RVLM), where sympathetic premotor neurons for the maintenance of vasomotor tone reside.

Methods: Stress-induced hypertension (SIH) was induced by electric foot-shock stressors with noise interventions in rats. Systolic blood pressure (SBP) and the power density of the low frequency (LF) component of the SAP spectrum were measured to reflect sympathetic vasomotor activity. Microglia activation and pro-inflammatory cytokines (PICs (IL-1β, TNF-α)) expression in the RVLM were measured by immunoblotting and immunostaining. Autophagy and autophagic vacuoles (AVs) were examined by autophagic marker (LC3 and p62) expression and transmission electron microscopy (TEM) image, respectively. Autophagy flux was evaluated by RFP-GFP-tandem fluorescent LC3 (tf-LC3) vectors transfected into the RVLM. Tissue levels of glutamate, gamma aminobutyric acid (GABA), and plasma levels of norepinephrine (NE) were measured by using high-performance liquid chromatography (HPLC) with electrochemical detection. The effects of the cisterna magna infused minocycline, a microglia activation inhibitor, on the abovementioned parameters were analyzed.

Results: SIH rats showed increased SBP, plasma NE accompanied by an increase in LF component of the SBP spectrum. Microglia activation and PICs expression was increased in SIH rats. TEM demonstrated that stress led to the accumulation of AVs in the RVLM of SIH rats. In addition to the Tf-LC3 assay, the concurrent increased level of LC3-II and p62 suggested the impairment of autophagic flux in SIH rats. To the contrary, minocycline facilitated autophagic flux and induced a hypotensive effect with attenuated microglia activation and decreased PICs in the RVLM of SIH rats. Furthermore, SIH rats showed higher levels of glutamate and lower level of GABA in the RVLM, while minocycline attenuated the decrease in GABA and the increase in glutamate of SIH rats.

Conclusions: Collectively, we concluded that the neuroinflammation might impair autophagic flux and induced neural excitotoxicity in the RVLM neurons following SIH, which is involved in the development of SIH.
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http://dx.doi.org/10.1186/s12974-017-0942-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569471PMC
August 2017

Spiraling elliptic solitons in lossy nonlocal nonlinear media.

Opt Express 2017 May;25(10):11717-11724

We address the propagation dynamics of the spiraling elliptic beams in nonlocal nonlinear media with losses based on the variational approach. It is found that the spiraling elliptic beams exhibit complicated behaviors, which result from the combined effects of the losses and orbital angular momentum (OAM). The OAM brings in an effective anisotropic diffraction and rotation for the spiraling elliptic beams. However, due to the losses, the rotation of the spiraling beams slows down. Besides, the ellipticity of the spiraling elliptic beams is greatly affected by the lossesand the OAM. When the OAM is not equal to its critical value, a periodic oscillation of the ellipticity is found in the presence of losses. However, when the OAM is equal to the critical one, the ellipticity of the spiraling elliptic beam remains unchanged during propagation regardless of the loss factor. The comparisons between our approximate analytic solutions and numerical simulations confirm our results.
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http://dx.doi.org/10.1364/OE.25.011717DOI Listing
May 2017

The adsorption and diffusion behavior of noble metal adatoms (Pd, Pt, Cu, Ag and Au) on a MoS monolayer: a first-principles study.

Phys Chem Chem Phys 2017 Aug;19(31):20713-20722

School of Electrical and Electronic Information, Shangqiu Normal University, Shangqiu 476000, P. R. China.

First-principles calculations based on periodic density functional theory (DFT) have been used to investigate the geometries, electronic structures, magnetic properties and diffusion behaviors of different noble metal adatoms (Pd, Pt, Cu, Ag and Au) on MoS monolayers. The results demonstrate that these adatoms can chemically adsorb on MoS monolayers. The band gaps of MoS monolayers with a Pd or Pt atom adsorbed are reduced owing to impurity states that emerge simultaneously within the gap region of the pristine MoS monolayer. The unpaired electrons in MoS monolayers with a Cu, Ag or Au atom adsorbed are spin polarized, resulting in total magnetic moments of 1.0 μ per supercell, which is caused by the strong hybridization between the metal adatoms and surrounding Mo or S atoms. Long-range antiferromagnetic (AFM) coupling has been observed between group IB metal adatoms. Due to charge transfer between adatoms and the MoS host, the work functions were modulated upon adsorption of noble metals. In addition, the diffusion behaviors of noble metal adatoms on the MoS monolayer suggest that Cu, Pd and Pt atoms favor the formation of a metal nanotemplate on the MoS monolayer, and Ag and Au are likely to form isolated particles in the initial growth stage. These findings may provide useful guidance to extend the potential applications of MoS in low-dimensional nanoelectronic and spintronic devices.
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http://dx.doi.org/10.1039/c7cp04021kDOI Listing
August 2017

Emodin alleviates bleomycin-induced pulmonary fibrosis in rats.

Toxicol Lett 2016 Nov 4;262:161-172. Epub 2016 Oct 4.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China; Shanghai Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention, Fudan University, Shanghai 200032, China. Electronic address:

Idiopathic pulmonary fibrosis (IPF) is a lethal lung disease with few treatment options and poor prognosis. Emodin, extracted from Chinese rhubarb, was found to be able to alleviate bleomycin (BLM)-induced pulmonary fibrosis, yet the underlying mechanism remains largely unknown. This study aimed to further investigate the effects of emodin on the inflammation and fibrosis of BLM-induced pulmonary fibrosis and the mechanism involved in rats. Our results showed that emodin improved pulmonary function, reduced weight loss and prevented death in BLM-treated rats. Emodin significantly relieved lung edema and fibrotic changes, decreased collagen deposition, and suppressed the infiltration of myofibroblasts [characterized by expression of α-smooth muscle actin (α-SMA)] and inflammatory cells (mainly macrophages and lymphocytes). Moreover, emodin reduced levels of TNF-α, IL-6, TGF-β1 and heat shock protein (HSP)-47 in the lungs of BLM-treated rats. In vitro, emodin profoundly inhibited TGF-β1-induced α-SMA, collagen IV and fibronectin expression in human embryo lung fibroblasts (HELFs). Emodin also inhibited TGF-β1-induced Smad2/3 and STAT3 activation, indicating that Smad2/3 and STAT3 inactivation mediates emodin-induced effects on TGF-β1-induced myofibroblast differentiation. These results suggest that emodin can exert its anti-fibrotic effect via suppression of TGF-β1 signaling and subsequently inhibition of inflammation, HSP-47 expression, myofibroblast differentiation and extracellular matrix (ECM) deposition.
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http://dx.doi.org/10.1016/j.toxlet.2016.10.004DOI Listing
November 2016

Response to 'Letter to "Application effect of sterile normal saline ice for post-partum hemorrhage at the time of cesarean delivery: A retrospective review": Is it safe?'

J Obstet Gynaecol Res 2016 Oct;42(10):1411

Department of Obstetrics and Gynecology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

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http://dx.doi.org/10.1111/jog.13134DOI Listing
October 2016

Variations of fine particle physiochemical properties during a heavy haze episode in the winter of Beijing.

Sci Total Environ 2016 Nov 25;571:103-9. Epub 2016 Jul 25.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China. Electronic address:

Chemical composition, morphology, size and mixture of fine particles were measured in a heavy haze and the post-haze air in Beijing in January 2012. With the occurrence of haze, the concentrations of gaseous and particulate pollutants including organics, sulfate, nitrate, and ammonium grew gradually. The hourly averaged PM2.5 concentration increased from 118μgm(-3) to 402μgm(-3) within 12h. In contrast, it was less than 10μgm(-3) in the post-haze air. Occupying approximately 46% in mass, organics were the major component of PM1 in both the haze and post-haze air. Analysis of individual particles in the size range of 0.2-1.1μm revealed that secondary-like particles and soot particles were always the majority, and most soot particles had a core-shell structure. The number ratio of secondary-like particles to soot particles in accumulation mode in the haze air was about 2:1, and that in the post-haze air was 8:1. These results indicate both secondary particle formation and primary emission contributed substantially to the haze. The mode size of the haze particles was about 0.7μm, and the mode size of the post-haze particles was 0.4μm, indicating the remarkable growth of particles in haze. However, the ratios of the core size to shell size of core-shell structure soot particles in the haze were similar to those in the post-haze air, suggesting a quick aging of soot particles in either the haze air or the post-haze air.
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http://dx.doi.org/10.1016/j.scitotenv.2016.07.147DOI Listing
November 2016

Application effect of sterile normal saline ice for post-partum hemorrhage at the time of cesarean delivery: A retrospective review.

J Obstet Gynaecol Res 2016 Oct 9;42(10):1286-1289. Epub 2016 Jun 9.

Department of Obstetrics and Gynecology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Aim: Post-partum hemorrhage (PPH) is a common complication of cesarean sections (CS) and affects maternal and newborn health. We used a new method to control bleeding and compared its efficacy with conventional methods.

Methods: Eighty-six women who experienced PPH with volume of bleeding over 1000 mL in CS between January 2008 and January 2012 were chosen as samples. Thirty-three underwent the new method in which normal saline ice blocks are placed in the uterus, and 53 underwent the conventional method. We evaluated blood loss, volume of transfusion and complications.

Results: Patients who were treated with ice blocks had better hemostatic efficacy than those who underwent the conventional method (1450 ± 251.9 mL vs 1800 ± 278.9 mL; P < 0.001); they also had less blood transfusion (806.1 ± 242.3 mL vs 1222.6 ± 308.0 mL; P < 0.001), lower rate of hysterectomy and infection (3.03% vs 5.66%; P = 0.971; 0% vs 7.55%; P = 0.276), and shorter duration of hospital stay (5.3 ± 0.5 days vs 7.6 ± 3.0 days; P < 0.001).

Conclusion: PPH in CS can be treated with peeled sterile normal saline ice blocks, a simple and reliable method for stopping bleeding. Nonetheless, there needs to be a large randomized control trial for confirmation.
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http://dx.doi.org/10.1111/jog.13047DOI Listing
October 2016

Regulatory Effect of Iguratimod on the Balance of Th Subsets and Inhibition of Inflammatory Cytokines in Patients with Rheumatoid Arthritis.

Mediators Inflamm 2015 2;2015:356040. Epub 2015 Dec 2.

Department of Immunology and Microbiology, Shanghai JiaoTong University School of Medicine, Shanghai Institute of Immunology, Shanghai 200025, China.

Objective: To expand upon the role of iguratimod (T-614) in the treatment of rheumatoid arthritis (RA), we investigated whether the Th1, Th17, follicular helper T cells (Tfh), and regulatory T cells (Treg) imbalance could be reversed by iguratimod and the clinical implications of this reversal.

Methods: In this trial, 74 patients were randomized into iguratimod-treated (group A) and control (broup B) group for a 24-week treatment period. In the subsequent 28 weeks, both groups were given iguratimod. Frequencies of Th1, Th17, Tfh, and Treg were quantified using flow cytometry, and serum cytokines were detected by enzyme-linked immunosorbent assay. mRNA expression of cytokines and transcriptional factor were quantified by RT-PCR. The composite Disease Activity Score, erythrocyte sedimentation rate, and C-reactive protein were assessed at each visit.

Result: The clinical scores demonstrated effective suppression of disease after treatment with iguratimod. In addition, iguratimod downregulated Th1, Th17-type response and upregulated Treg. Furthermore, the levels of Th1, Th17, and Tfh associated inflammatory cytokines and transcription factors were reduced after treatment with iguratimod, while the levels of Treg associated cytokines and transcription factors were increased.
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http://dx.doi.org/10.1155/2015/356040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4680115PMC
September 2016

Spiraling elliptic beam in nonlocal nonlinear media.

Opt Express 2015 Sep;23(19):24612-25

Analytically discussed is the dynamical properties of the spiraling elliptic beams in nonlocal nonlinear media. This class of spiraling elliptic beams carry the orbital angular momentum (OAM), and can rotate on the cross section perpendicular to the propagation direction during the propagations. The optical intensity, the beam width, and specially the angular velocity are both analytically and numerically discussed in details. We shown that both the deviations from the critical power and the deviations from the critical OAM can make the spiraling elliptic beams breathe. The decrease (increase) of the OAM or the increase (decrease) of the power can both make the spiraling elliptic breathers contract (diffract), however, there still exist differences between them. The rotating speed can be changed by the input optical power or the input OAM, which may have potential applications in the controlling of the optical beams.
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http://dx.doi.org/10.1364/OE.23.024612DOI Listing
September 2015

Berberine ameliorates TNBS induced colitis by inhibiting inflammatory responses and Th1/Th17 differentiation.

Mol Immunol 2015 Oct 26;67(2 Pt B):444-54. Epub 2015 Jul 26.

Department of Immunology and Microbiology, Shanghai JiaoTong University School of Medicine, Shanghai Institute of Immunology, Shanghai, China. Electronic address:

Th1 and Th17 cells, and their associated cytokines, have been associated with the pathogenesis of Crohn's disease. Berberine (BBR), a compound long used in traditional Chinese medicines, has been reported to have therapeutic effects in treating experimental colitis. In this study, we show that BBR had a protective effect on mice with TNBS-induced colitis. BBR inhibited levels of IFN-γ, IL-17, IL-6, IL-1β and TNF-α both in the local colon and sera, and transiently increased levels of IL-22. BBR also markedly increased sIgA expression in the colon. BBR had pronounced effects on macrophage populations. Treatment with BBR adjusted the M2/M1 ratio. In addition, BBR exerted effects on adaptive immunity by suppressing numbers of Th1 and Th17 cells, as well as expression levels of their associated cytokines and transcriptional factors. BBR downregulated STAT3 and STAT1 phosphorylation, and inhibited phosphorylation of NF-kB. In vitro experiments showed that BBR inhibited the differentiation of Th17 and, to a lesser degree, Th1 cells, without affecting regulatory T cells. Therefore, we conclude that BBR plays a regulatory role in modulating the balance of immune responses in TNBS-induced colitis. Our study will help us understand the regulatory mechanisms exerted by BBR in the treatment of IBD.
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http://dx.doi.org/10.1016/j.molimm.2015.07.013DOI Listing
October 2015
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