Publications by authors named "Wenjie Yang"

158 Publications

Maladaptive Perfectionism and Internet Addiction among Chinese College Students: A Moderated Mediation Model of Depression and Gender.

Int J Environ Res Public Health 2021 03 9;18(5). Epub 2021 Mar 9.

Mental Health Education Center for College Students, Zhejiang Gongshang University, Hangzhou 310018, China.

The association between perfectionism and addictive behaviors has been examined in previous literature; however, few pieces of research have investigated the mediating and moderating mechanisms underlying this relationship. Using a sample of 2016 Chinese college students, the present study examined the mediator of depression between maladaptive perfectionism and Internet addiction and the moderator of gender in such associations. The findings indicated that maladaptive perfectionism was directly related to students' Internet addiction and indirectly predicted students' Internet addiction via the mediator of depression. Gender moderated the direct effect, rather than the indirect effect, of maladaptive perfectionism on Internet addiction. Even though males reported a lower score on Internet addiction compared to females, the effect of maladaptive perfectionism on Internet addiction was stronger for males than for females. These findings revealed the psychological mechanisms from perfectionism to Internet addiction, which contributed to the theoretical development in addiction research and provided implications for interventions to reduce Internet addiction among Chinese college students.
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http://dx.doi.org/10.3390/ijerph18052748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967480PMC
March 2021

The oncogene Mct-1 promotes progression of hepatocellular carcinoma via enhancement of Yap-mediated cell proliferation.

Cell Death Discov 2021 Mar 22;7(1):57. Epub 2021 Mar 22.

Hepatobiliary Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.

Malignant T-cell-amplified sequence 1 (Mct-1) has been reported as an oncogene in multiple malignant diseases. However, the function of Mct-1 in hepatocellular carcinoma (HCC) and the molecular mechanisms underlying tumor progression have not been explored. In this study, Mct-1 expression levels in HCC tissues and cells were detected by quantitative real-time PCR and western blotting. Mct-1 shRNAs and overexpression vector were transfected into HCC cells to downregulate or upregulate Mct-1 expression. In vitro and in vivo assays were performed to investigate the function of Mct-1 in cell proliferation and apoptosis. RNA sequencing analysis (RNA-seq) was performed to explore differences in gene expression when silenced Mct-1 expression. Mct-1 was upregulated in HCC specimens and cell lines, and higher expression of Mct-1 was predictive of poor survival. Overexpression of Mct-1 was shown to promote cell proliferation and repress cell apoptosis both in vitro and in vivo. The results of RNA-seq indicated that knockdown of Mct-1 suppressed Yap expression, while the results of the luciferase assay also revealed that Mct-1 increases the activity of the Yap promoter. Restoration of Yap expression in Mct-1 knockdown cells partially recovered the promotion of cell proliferation and inhibition of apoptosis. Collectively, these results indicate that Mct-1 acts as a tumor promoter gene in HCC progression by up-regulating Yap expression and, thus, could serve a novel potential diagnostic and prognostic biomarker for HCC.
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http://dx.doi.org/10.1038/s41420-021-00413-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985373PMC
March 2021

Hydrogen sulfide treatment at the late growth stage of extends chronological lifespan.

Aging (Albany NY) 2021 Mar 19;13. Epub 2021 Mar 19.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, China.

Previous studies demonstrated that lifelong treatment with a slow HS releasing donor extends yeast chronological lifespan (CLS), but it is not clear when the action of HS benefits to CLS during yeast growth. Here, we show that short HS treatments by using NaHS as a fast HS releasing donor at 96 hours after inoculation extended yeast CLS while NaHS treatments earlier than 72 hours after inoculation failed to do so. To reveal the mechanism, we analyzed the transcriptome of yeast cells with or without the early and late NaHS treatments. We found that both treatments had similar effects on pathways related to CLS regulation. Follow-up qPCR and ROS analyses suggest that altered expression of some antioxidant genes by the early NaHS treatments were not stable enough to benefit CLS. Moreover, transcriptome data also indicated that some genes were regulated differently by the early and late HS treatment. Specifically, we found that the expression of , a human homolog and also a key regulator of the yeast cell wall synthesis, was significantly altered by the late NaHS treatment but not altered by the early NaHS treatment. Finally, the key role of in CLS regulation by HS is revealed by CLS data showing that the late NaHS treatment did not enhance the CLS of a knockout mutant. This study sheds light on the molecular mechanism of CLS extension induced by HS, and for the first time addresses the importance of HS treatment timing for lifespan extension.
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http://dx.doi.org/10.18632/aging.202738DOI Listing
March 2021

Imaging cerebral microbleeds in Cushing's disease evaluated by quantitative susceptibility mapping: an observational cross-sectional study.

Eur J Endocrinol 2021 Apr;184(4):565-574

Department of Neurosurgery, Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Design: Cushing's disease (CD) is a rare clinical syndrome characterized by chronic exposure to hypercortisolism due to an adrenocorticotropic hormone-secreting pituitary adenoma. The adverse effects of chronic exposure to hypercortisolism on the human brain remain unclear. The purpose of this study was to assess the prevalence of cerebral microbleeds (CMBs) in CD patients and their associations with clinical characteristics.

Methods: In this study, 48 active CD patients, 39 remitted CD patients, and 52 healthy control (HC) subjects underwent MRI. CD patients also underwent neuropsychological testing and clinical examinations. The number, locations, and volumes of CMBs were assessed on quantitative susceptibility mapping (QSM) images and with the Microbleed Anatomical Rating Scale. The correlation between CMBs and clinical characteristics was explored.

Results: The prevalence of CMBs among active and remitted CD patients was higher than that among HCs (16.3%, 20.5%, and 3.3%, respectively). Moreover, the age of CD patients with CMBs were much younger than HCs with CMBs. Furthermore, the increased number of CMBs in active CD patients was associated with increased cerebrospinal fluid (CSF) volumes in remitted CD patients.

Conclusions: Chronic exposure to hypercortisolism may be relevant to CMBs and significantly correlated with altered brain volumes in CD.
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http://dx.doi.org/10.1530/EJE-20-1139DOI Listing
April 2021

A perfusable, multifunctional epicardial device improves cardiac function and tissue repair.

Nat Med 2021 03 15;27(3):480-490. Epub 2021 Mar 15.

Department of Cardiovascular Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Despite advances in technologies for cardiac repair after myocardial infarction (MI), new integrated therapeutic approaches still need to be developed. In this study, we designed a perfusable, multifunctional epicardial device (PerMed) consisting of a biodegradable elastic patch (BEP), permeable hierarchical microchannel networks (PHMs) and a system to enable delivery of therapeutic agents from a subcutaneously implanted pump. After its implantation into the epicardium, the BEP is designed to provide mechanical cues for ventricular remodeling, and the PHMs are designed to facilitate angiogenesis and allow for infiltration of reparative cells. In a rat model of MI, implantation of the PerMed improved ventricular function. When connected to a pump, the PerMed enabled targeted, sustained and stable release of platelet-derived growth factor-BB, amplifying the efficacy of cardiac repair as compared to the device without a pump. We also demonstrated the feasibility of minimally invasive surgical PerMed implantation in pigs, demonstrating its promise for clinical translation to treat heart disease.
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http://dx.doi.org/10.1038/s41591-021-01279-9DOI Listing
March 2021

Dysregulation of miR-23b-5p promotes cell proliferation via targeting FOXM1 in hepatocellular carcinoma.

Cell Death Discov 2021 Mar 15;7(1):47. Epub 2021 Mar 15.

Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University; Key Laboratory of Liver Transplantation, Chinese Academy of Medical Sciences, NHC Key Laboratory of Liver Transplantation, Nanjing, China.

Growing evidence demonstrates that MicroRNAs (miRNAs) play an essential role in contributing to tumor development and progression. However, the underlying role and mechanisms of miR-23b-5p in hepatocellular carcinoma (HCC) formation remain unclear. Our study showed that miR-23b-5p was downregulated in the HCC tissues and cell lines, and lower expression of miR-23b-5p was associated with more severe tumor size and poorer survival. Gain- or loss-of-function assays demonstrated that miR-23b-5p induced G0/G1 cell cycle arrest and inhibited cell proliferation both in vitro and in vivo. qRT-PCR, western blot and luciferase assays verified that Mammalian transcription factor Forkhead Box M1 (FOXM1), upregulated in HCC specimens, was negatively correlated with miR-23b-5p expression and acted as a direct downstream target of miR-23b-5p. In addition, miR-23b-5p could regulate cyclin D1 and c-MYC expression by directly targeting FOXM1. Further study revealed that restoration of FOXM1 neutralized the cell cycle arrest and cell proliferation inhibition caused by miR-23b-5p. Taken together, our findings suggest that miR-23b-5p acted as a tumor suppressor role in HCC progression by targeting FOXM1 and may serve as a potential novel biomarker for HCC diagnosis and prognosis.
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http://dx.doi.org/10.1038/s41420-021-00440-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960996PMC
March 2021

Naoxintong accelerates diabetic wound healing by attenuating inflammatory response.

Pharm Biol 2021 Dec;59(1):252-261

State Key Laboratory of Component-based Chinese Medicine, Tianjin, China.

Context: Naoxintong (NXT), a prescribed traditional Chinese medicine, widely used in cerebrovascular and cardiovascular diseases, could be effective in diabetic wounds.

Objective: This study evaluates the wound healing activity of NXT by employing an excisional wound splinting model.

Materials And Methods: NXT was dissolved in saline and given daily by gavage. Wounds were induced at the dorsum of non-diabetic (db/+) and diabetic (db/db) mice and treated with saline or 700 mg/kg/d NXT for 16 days. Wound closure was measured every four days. Extracellular matrix (ECM) remodelling, collagen deposition, leukocyte infiltration and expression of Col-3, CK14, CXCL1, CXCL2, MPO, Ly6G, CD68, CCR7, CD206, p-JAK1, p-STAT3 and p-STAT6 was analysed.

Results: NXT significantly accelerated rate of wound closure increased from 70% to 84%, accompanied by up-regulation of collagen deposition and ECM at days 16 post-injury. Moreover, NXT alleviated neutrophil infiltration, accompanied by down-regulation of CXCL1 and CXCL2 mRNA expression. In addition, NXT markedly augmented neutrophil efferocytosis. In diabetic wounds, the levels of M1 marker gene (CCR7) increased, while M2 marker gene (CD206) decreased, demonstrating a pro-inflammatory shift. Application of NXT increased M2 macrophage phenotype in db/db mice. Mechanistically, NXT treatment increased expression level of p-STAT3 and p-STAT6 at days 3 post-injury, indicating NXT mediated macrophages towards M2 phenotype and alleviated inflammation in diabetic wounds by activation of STAT3 and STAT6.

Conclusions: Our study provides evidence that NXT accelerates diabetic wound healing by attenuating inflammatory response, which provides an important basis for use of NXT in the treatment of chronic diabetic wound healing.
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http://dx.doi.org/10.1080/13880209.2021.1877735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946048PMC
December 2021

Formation and Location of Pt Single Sites Induced by Pentacoordinated Al Species on Amorphous Silica-Alumina.

J Phys Chem Lett 2021 Mar 8;12(10):2536-2546. Epub 2021 Mar 8.

School of Engineering, Macquarie University, Sydney, New South Wales 2109, Australia.

Alumina and its mixed oxides are popular industrial supports for emerging supported metal catalysts. Pentacoordinated Al (Al) species are identified as key surface sites for anchoring and stabilizing metal single-site catalysts; however, Al is rare in conventional amorphous silica-alumina (ASA). Recently, we have developed Al-enriched ASA, which was applied as a support for the synthesis of Pt single-site catalysts in this work. Each preparation stage and the interaction between Pt and surface Al species were explored by H and Al solid-state nuclear magnetic resonance spectroscopy, and the formation of the dominant Pt single sites on the surface of Al-enriched ASA was confirmed by high-angle annular dark-field imaging scanning transmission electron microscopy and energy dispersive spectroscopy line scanning. On the surface of supports without a significant Al population (Pt/AlO and Pt/SiO), mainly Pt nanoparticles were formed. This indicates that Al contributes to the strong metal-support interaction to stabilize the Pt single sites on Pt/ASA, which was characterized by diffuse reflectance infrared Fourier transform spectroscopy combined with CO adsorption, X-ray photoelectron spectroscopy, and electron energy loss spectroscopy. Pt single sites supported on Al-enriched ASA exhibit excellent chemoselectivity in the hydrogenation of C═O groups, affording 2-3-fold higher yields compared to those of Pt nanoparticles supported on AlO and SiO.
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http://dx.doi.org/10.1021/acs.jpclett.1c00139DOI Listing
March 2021

Epigenetically modulated miR-1224 suppresses the proliferation of HCC through CREB-mediated activation of YAP signaling pathway.

Mol Ther Nucleic Acids 2021 Mar 16;23:944-958. Epub 2021 Jan 16.

Hepatobiliary/Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Liver Transplantation, Chinese Academy of Medical Sciences, Nanjing 210029, China.

Mounting evidence has demonstrated that microRNA-1224 (miR-1224) is commonly downregulated and serves as a tumor suppressor in multiple malignancies. However, the role and mechanisms responsible for miR-1224 in hepatocellular carcinoma (HCC) remain unclear. In this study, we found that the expression of miR-1224 was downregulated in HCC. Low miR-1224 expression was associated with poor clinicopathologic features and short overall survival. Moreover, the methylation status of putative CpG islands was also found to be an important part in the modulation of miR-1224 expression. miR-1224 could induce HCC cells to arrest in G0/G1 phase and inhibited the proliferation of HCC cells both and Mechanistic investigation showed that by binding with cyclic AMP (cAMP)-response element binding protein (CREB) miR-1224 could repress the transcription and the activation of Yes-associated protein (YAP) signaling pathway. Furthermore, the expression of miR-1224 was inhibited by CREB through EZH2-mediated histone 3 lysine 27 (H3K27me3) on miR-1224 promoter, thus forming a positive feedback circuit. Our findings identify a miR-1224/CREB feedback loop for HCC progression and that blocking this circuit may represent a promising target for HCC treatment.
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http://dx.doi.org/10.1016/j.omtn.2021.01.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868928PMC
March 2021

Geographical distribution of reference values of fibrinogen degradation products in healthy adults in China.

Int J Biometeorol 2021 Feb 8. Epub 2021 Feb 8.

Institute of Health Geography, School of Geography and Tourism, Shaanxi Normal University, Xi'an, 710119, China.

To explore the reference values of fibrinogen degradation products (FDP) of healthy adults in different regions of China, determine the relationship between them and geographic factors and determine the geographic distribution of FDP reference value healthy adults in China. The collected FDP reference values of 11,013 healthy adults in 209 Chinese units were firstly determined by spatial autocorrelation to correlate with geographic factors; secondly, using ridge regression analysis and principal component analysis to fit China 2322 FDP reference values of healthy adults in each city, and the selection of the optimal model through comparison; and finally, combined with geostatistical analysis, explore the geographical distribution of FDP reference values of healthy adults in China. The specific distribution of FDP reference values of healthy adults in different regions of China showed a trend of being lower to the north and higher to the south of the Qinling-Huaihe River. If the numerical value of geographical factors in a certain region of China is known, the ridge regression prediction equation can be combined from this: Ŷ = 3.30 - 0.002900X - 0.0001400X + 0.0001300X - 0.009040X + 0.0003500X - 0.002300X + 0.02149X - 0.01626X ± 0.89 calculates the reference value of FDP for healthy adults in the region and provides a scientific reference for the FDP reference value in different regions of China.
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http://dx.doi.org/10.1007/s00484-021-02082-4DOI Listing
February 2021

Metabolomic analysis of plasma from normal-weight adults with hypo-HDL cholesterolemia by UPLC-QTOF MS.

Biomed Chromatogr 2021 Jan 16:e5073. Epub 2021 Jan 16.

College of Public Health, Zhengzhou University, Zhengzhou, Henan, China.

High-density lipoprotein cholesterol (HDL-C) is negatively correlated with atherosclerotic cardiovascular disease. The prevalence of hypo-HDL cholesterolemia is as high as 33.9%. The plasma metabolomic differences between hypo-HDL cholesterolemia populations and normal controls were investigated using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Participants with hypo-HDL cholesterolemia and normal controls were clearly discriminated from each other on the orthogonal partial least squares-discriminant analysis score plot and a total of 90 differential metabolites were identified, including down-regulated phosphatidylserine [18:0/20:3(8Z,11Z,14Z)], phosphatidylcholine [19:0/18:3(6Z,9Z,12Z)], phosphatidylserine, phosphatidylethanolamine [18:0/20:4(5Z,8Z,11Z,13E) (15Ke)], etc., and up-regulated triglyceride [15:0/18:1(9Z)/18:3(9Z,12Z,15Z)][iso6], 13-methyl-1-tritriacontene, tridodecylamine, etc. Most of the changed metabolites were lipids, notably, a significant part of which were odd chain fatty acid incorporated lipids. Carnitine shuttle was the most significant metabolic pathway, except for the disturbed glycerophospholipid metabolism, glycosphingolipid metabolism and sphingolipid metabolism in participants with hypo-HDL cholesterolemia. We identified the key metabolites and metabolic pathways that may be changed in hypo-HDL cholesterolemia participants, providing useful clues for studying the metabolic mechanisms and for early prevention of hypo-HDL cholesterolemia and dyslipidemia.
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http://dx.doi.org/10.1002/bmc.5073DOI Listing
January 2021

Shallow Graph Convolutional Network for Skeleton-Based Action Recognition.

Sensors (Basel) 2021 Jan 11;21(2). Epub 2021 Jan 11.

Key Laboratory of Optical Engineering, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China.

Graph convolutional networks (GCNs) have brought considerable improvement to the skeleton-based action recognition task. Existing GCN-based methods usually use the fixed spatial graph size among all the layers. It severely affects the model's abilities to exploit the global and semantic discriminative information due to the limits of receptive fields. Furthermore, the fixed graph size would cause many redundancies in the representation of actions, which is inefficient for the model. The redundancies could also hinder the model from focusing on beneficial features. To address those issues, we proposed a plug-and-play channel adaptive merging module (CAMM) specific for the human skeleton graph, which can merge the vertices from the same part of the skeleton graph adaptively and efficiently. The merge weights are different across the channels, so every channel has its flexibility to integrate the joints. Then, we build a novel shallow graph convolutional network (SGCN) based on the module, which achieves state-of-the-art performance with less computational cost. Experimental results on NTU-RGB+D and Kinetics-Skeleton illustrates the superiority of our methods.
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http://dx.doi.org/10.3390/s21020452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827280PMC
January 2021

Complete response to immunotherapy combined with an antiangiogenic agent in multiple hepatic metastases after radical surgery for advanced gallbladder cancer: a case report.

Ann Transl Med 2020 Dec;8(23):1609

Hepatobiliary Center, the First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Liver Transplantation, Research Unit of Liver Transplantation and Transplant Immunology, Chinese Academy of Medical Sciences, NHC Key Laboratory of Living Donor Liver Transplantation, Nanjing, China.

Most advanced gallbladder cancers (GBCa) are unresectable or metastatic once diagnosed, and even patients who undergo surgery have a high risk of recurrence and metastasis. Immunotherapy, especially immune checkpoint inhibitors (ICIs), combined with an antiangiogenic agent, is an emerging prospective treatment for GBCa. However, the efficacy and safety of this combination therapy have not yet been investigated. We report the case of a 70-year-old female patient with recurrent metastatic GBCa (stage IVB) after radical surgery. Immunohistochemical examination revealed that 10% of the tumor cells expressed programmed cell death protein-1 (PD-1) and programmed cell death receptor ligand 1 (PD-L1). Whole-exome sequencing showed cancer tissues with a low tumor mutational burden (TMB) and microsatellite stability (MSS). The patient received Camrelizumab (200 mg, every three weeks) and Apatinib (40 mg/d). The clinical and immunological responses were observed, and the patient achieved a complete response after five cycles. This is the first case describing the efficacy and safety of Camrelizumab plus Apatinib in a GBCa patient with weak PD-1 and PD-L1 expression, and low TMB and MSS. The treatment had a tolerable safety profile and a complete response in the patient. Also, we found that the cluster of differentiation (CD)16+CD56+natural killer (NK) cell ratio in peripheral blood was increased after the combined treatment. Immunotherapy with antiangiogenic drugs may be a potential treatment option for patients with recurrent GBC or GBCa.
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http://dx.doi.org/10.21037/atm-20-4420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791255PMC
December 2020

Does soluble starch improve the removal of Cr(VI) by nZVI loaded on biochar?

Ecotoxicol Environ Saf 2021 Jan 8;208:111552. Epub 2020 Nov 8.

Chinese Academy for Environmental Planning, Beijing 100012, China; College of Renewable Energy, North China Electric Power University, Beijing 102206, China. Electronic address:

A novel material that nano zero valent iron (nZVI) loaded on biochar with stable starch stabilization (nZVI/SS/BC) was synthesized and used for the removal of hexavalent chromium [Cr(VI)] in simulated wastewater. It was indicated that as the pyrolysis temperature of rice straw increased, the removal rate of Cr(VI) by nZVI/SS/BC first increased and then decreased. nZVI/SS/BC made from biochar pyrolyzed at 600 °C (nZVI/SS/BC600) had the highest removal efficiency and was suitable for a wide pH range (pH 2.1-10.0). The results showed that 99.67% of Cr(VI) was removed by nZVI/SS/BC600, an increase of 45.93% compared to the control group, which did not add soluble starch during synthesis. The pseudo-second-order model and the Langmuir model were more in line with reaction. The maximum adsorption capacity for Cr(VI) by nZVI/SS/BC600 was 122.86 mg·g. The properties of the material were analyzed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) mapping, Brunauer-Emmett-Teller (BET), Fourier-transform infrared (FTIR), and X-ray diffraction (XRD). The results showed that the nZVI particles were uniformly supported on the biochar, and the BET surface areas of nZVI/SS/BC was 40.4837 m·g, an increase of 8.79 times compared with the control group. Mechanism studies showed that soluble starch reduced the formation of metal oxides, thereby improving the reducibility of the material, and co-precipitates were formed during the reaction. All results indicated that nZVI/SS/BC was a potential repair material that can effectively overcome the limitations of nZVI and achieve efficient and rapid repair of Cr(VI).
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http://dx.doi.org/10.1016/j.ecoenv.2020.111552DOI Listing
January 2021

A chromosome-scale reference genome of Lobularia maritima, an ornamental plant with high stress tolerance.

Hortic Res 2020 Dec 1;7(1):197. Epub 2020 Dec 1.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, 610065, Chengdu, China.

Lobularia maritima (L.) Desv. is an ornamental plant cultivated across the world. It belongs to the family Brassicaceae and can tolerate dry, poor and contaminated habitats. Here, we present a chromosome-scale, high-quality genome assembly of L. maritima based on integrated approaches combining Illumina short reads and Hi-C chromosome conformation data. The genome was assembled into 12 pseudochromosomes with a 197.70 Mb length, and it includes 25,813 protein-coding genes. Approximately 41.94% of the genome consists of repetitive sequences, with abundant long terminal repeat transposable elements. Comparative genomic analysis confirmed that L. maritima underwent a species-specific whole-genome duplication (WGD) event ~22.99 million years ago. We identified ~1900 species-specific genes, 25 expanded gene families, and 50 positively selected genes in L. maritima. Functional annotations of these genes indicated that they are mainly related to stress tolerance. These results provide new insights into the stress tolerance of L. maritima, and this genomic resource will be valuable for further genetic improvement of this important ornamental plant.
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http://dx.doi.org/10.1038/s41438-020-00422-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705659PMC
December 2020

Circular RNA circGSK3B Promotes Cell Proliferation, Migration, and Invasion by Sponging miR-1265 and Regulating Expression in Hepatocellular Carcinoma.

Front Oncol 2020 11;10:598256. Epub 2020 Nov 11.

Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, China.

Circular RNAs (circRNAs) have important regulatory roles in the development of various cancers. However, the biological functions and potential molecular mechanisms of circRNAs in hepatocellular carcinoma (HCC) are still unclear. In this study, we investigated the role of a new circRNA-circGSK3B (hsa_circ_0003763) and its molecular mechanism in HCC. We found that circGSK3B was highly expressed in HCC tissues and HCC cell lines. Additionally, the expression level of circGSK3B significantly correlated with HCC tumor size and vascular invasion. Functionally, we confirmed that circGSK3B can promote the proliferation, migration, and invasion of HCC cells and . In terms of mechanism, we demonstrated that circGSK3B acts as a miR-1265 sponge, positively regulates the target gene , and promotes the reprogramming of glutamine metabolism, thereby promoting the progression of HCC. Finally, the classic RNA binding protein QKI was observed to participate in the biogenesis of circGSK3B. In summary, we proved that the circGSK3B-miR-1265- axis can promote the proliferation, migration, invasion of HCC cells, indicating that circGSKB may serve as a promising diagnostic and prognostic marker in HCC.
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http://dx.doi.org/10.3389/fonc.2020.598256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688052PMC
November 2020

Disrupted Patterns of Rich-Club and Diverse-Club Organizations in Subjective Cognitive Decline and Amnestic Mild Cognitive Impairment.

Front Neurosci 2020 15;14:575652. Epub 2020 Oct 15.

Institute of Brain Functional Imaging, Nanjing Medical University, Nanjing, China.

Background: Subjective cognitive decline (SCD) and amnestic mild cognitive impairment (aMCI) were considered to be a continuum of Alzheimer's disease (AD) spectrum. The abnormal topological architecture and rich-club organization in the brain functional network can reveal the pathology of the AD spectrum. However, few studies have explored the disrupted patterns of diverse club organizations and the combination of rich- and diverse-club organizations in SCD and aMCI.

Methods: We collected resting-state functional magnetic resonance imaging data of 19 SCDs, 29 aMCIs, and 28 healthy controls (HCs) from the Alzheimer's Disease Neuroimaging Initiative. Graph theory analysis was used to analyze the network metrics and rich- and diverse-club organizations simultaneously.

Results: Compared with HC, the aMCI group showed altered small-world and network efficiency, whereas the SCD group remained relatively stable. The aMCI group showed reduced rich-club connectivity compared with the HC. In addition, the aMCI group showed significantly increased feeder connectivity and decreased local connectivity of the diverse club compared with the SCD group. The overlapping nodes of the rich club and diverse club showed a significant difference in nodal efficiency and shortest path length () between groups. Notably, the values of overlapping nodes in the SCD and aMCI groups were significantly associated with episodic memory.

Conclusion: The present study demonstrates that the network properties of SCD and aMCI have varying degrees of damage. The combination of the rich club and the diverse club can provide a novel insight into the pathological mechanism of the AD spectrum. The altered patterns in overlapping nodes might be potential biomarkers in the diagnosis of the AD spectrum.
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http://dx.doi.org/10.3389/fnins.2020.575652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593791PMC
October 2020

CCL5-dependent mast cell infiltration into the tumor microenvironment in clear cell renal cell carcinoma patients.

Aging (Albany NY) 2020 11 11;12(21):21809-21836. Epub 2020 Nov 11.

Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, P.R. China.

We investigated the mechanisms affecting tumor progression and survival outcomes in -mutated () clear cell renal cell carcinoma (ccRCC) patients. ccRCC tissues contained higher numbers of mast cells and lower numbers of CD8 and CD4 T cells than tissues from ccRCC patients. Hierarchical clustering, pathway enrichment and GSEA analyses demonstrated that mutations promote tumor progression by activating hypoxia inducible factor (HIF)-related signaling pathways and increasing expression of vascular endothelial growth factor family genes. ccRCC tissues also show increased expression of C-C motif chemokine ligand 5 (CCL5). PBRM1-silenced ccRCC cells exhibited greater Matrigel tube formation and cell proliferation than controls. In addition, HMC-1 human mast cells exhibited CCL5-dependent migration on Transwell plates. High CCL5 expression in ccRCC patients correlated with increased expression of genes encoding IFN-γ, IFN-α, IL-6, JAK-STAT3, TNF-α, and NF-ΚB. Moreover, high CCL5 expression was associated with poorer survival outcomes in ccRCC patients. These findings demonstrate that CCL5-dependent mast cell infiltration promotes immunosuppression within the tumor microenvironment, resulting in tumor progression and adverse survival outcomes in ccRCC patients.
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http://dx.doi.org/10.18632/aging.103999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695370PMC
November 2020

The chromosome-level genome sequence and karyotypic evolution of Megadenia pygmaea (Brassicaceae).

Mol Ecol Resour 2021 Apr 1;21(3):871-879. Epub 2020 Dec 1.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.

Karyotypic changes in chromosome number and structure are drivers in the divergent evolution of diverse plant species and lineages. This study aimed to reveal the origins of the unique karyotype (2n = 12) and phylogenetic relationships of the genus Megadenia (Brassicaceae). A high-quality chromosome-scale genome was assembled for Megadenia pygmaea using Nanopore long reads and high-throughput chromosome conformation capture (Hi-C). The assembled genome is 215.2 Mb and is anchored on six pseudochromosomes. We annotated a total of 25,607 high-confidence protein-coding genes and corroborated the phylogenetic affinity of Megadenia with the Brassicaceae expanded lineage II, containing numerous agricultural crops. We dated the divergence of Megadenia from its closest relatives to 27.04 (19.11-36.60) million years ago. A reconstruction of the chromosomal composition of the species was performed based on the de novo assembled genome and comparative chromosome painting analysis. The karyotype structure of M. pygmaea is very similar to the previously inferred proto-Calepineae karyotype (PCK; n = 7) of the lineage II. However, an end-to-end translocation between two ancestral chromosomes reduced the chromosome number from n = 7 to n = 6 in Megadenia. Our reference genome provides fundamental information for karyotypic evolution and evolutionary study of this genus.
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http://dx.doi.org/10.1111/1755-0998.13291DOI Listing
April 2021

Nogo-B is a key mediator of hepatic ischemia and reperfusion injury.

Redox Biol 2020 10 8;37:101745. Epub 2020 Oct 8.

Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University, Research Unit of Liver Transplantation and Transplant Immunology, Chinese Academy of Medical Sciences, Key Laboratory of Liver Transplantation, Chinese Academy of Medical Sciences, NHC Key Laboratory of Living Donor Liver Transplantation, Nanjing, 210029, China. Electronic address:

Nogo-B is an endoplasmic reticulum-residential protein with distinctive functions in different diseases. However, it remains unclear the role of Nogo-B in liver sterile inflammatory injury. This study aims to elucidate the functions and mechanisms in liver ischemia and reperfusion injury (IRI). The Nogo-B expression and liver function were analyzed in biopsy/serum specimens from 36 patients undergoing ischemia-related hepatectomy and in a mouse model of liver IRI. Human specimens were harvested prior to ischemia and post-reperfusion. The Nogo-B knockout (Nogo-B) and myeloid-specific Nogo-B knockout (Nogo-B) mice were used to analyze the function and mechanism of Nogo-B in a mouse model of liver IRI. In human specimens, the Nogo-B expression was positively correlated with higher levels of serum transaminase (sALT) and severe histopathological injury at one day post-hepatectomy. Moreover, Nogo-B is mainly expressed on macrophages in normal and ischemic liver tissues from human and mice. Unlike in controls, the Nogo-B or Nogo-B livers was protected against IRI, with reduced reactive oxygen species (ROS) production and liver inflammation in ischemic livers. In parallel in vitro studies, Nogo-B deficiency reduced M1 macrophage polarization and inhibited proinflammatory cytokines (TNF-α, IL-6, MCP-1 and iNOS) in response to LPS or HMGB-1 stimulation. Mechanistic studies showed that Nogo-B bound to MST1/2, increased MST1/2, LAST1, and YAP phosphorylation, leading to reduced YAP activity. Interestingly, disruption of macrophage YAP abolished Nogo-B deficiency-mediated cytoprotective effects in vitro and in vivo. Thus, YAP is crucial for the regulation of macrophage Nogo-B-triggered liver inflammation. Nogo-B promotes macrophage-related innate inflammation and contributes to IR-induced liver injury by activating the MST-mediated Hippo/YAP pathway, which provides a potential therapeutic target for clinical management in liver IRI.
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http://dx.doi.org/10.1016/j.redox.2020.101745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582106PMC
October 2020

Inhibition of Androgen Receptor Signaling Promotes Prostate Cancer Cell Migration via Upregulation of Annexin A1 Expression.

Arch Med Res 2021 02 13;52(2):174-181. Epub 2020 Oct 13.

Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, PR China; Key Laboratory for Tumor Precision Medicine of Shaanxi Province, Xi'an, PR China. Electronic address:

Background: Recent studies indicate that androgen deprivation therapy (ADT), the main therapeutic approach for metastatic prostate cancer (PCa), accelerates PCa invasion and metastasis. Annexin A1 (ANXA1) is a Ca-regulated phospholipid-binding protein that can promote PCa migration and invasion.

Aim Of The Study: The aim of this study is to determine whether ANXA1 is regulated by ADT and participates in PCa progression after ADT, and to explore the possible mechanism of ANXA1-mediated PCa migration.

Methods: Expression of ANXA1 and androgen receptor (AR) in PCa cell lines and tissues was detected, and the association between these two proteins were analyzed. Expression of ANXA1 was evaluated after AR knockdown or AR inhibition in PCa cell lines. Cell migration of PCa cell liness after ANXA1 knockdown or overexpression was determined by in vitro migration assay. Transcriptome analysis was used to explore the possible mechanism of ANXA1-mediated PCa migration.

Results: ANXA1 expression in PCa cell lines and tissues was reversely associated with AR. In vitro studies revealed an increase in ANXA1 expression after AR knockdown or treatment with AR antagonist. Moreover, functional assays indicated that ANXA1 knockdown in PCa cells significantly inhibited cell migration, while ANXA1 overexpression in PCa cells significantly accelerated cell migration. Transcriptome analysis showed that ANXA1 regulated multiple genes involved in cell junction organization, such as CADM1, LIMCH1 and PPM1F.

Conclusions: Our results indicate that ADT might accelerate PCa metastasis via ANXA1 expression and PCa cell migration.
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http://dx.doi.org/10.1016/j.arcmed.2020.10.005DOI Listing
February 2021

Correction: Pd-Catalyzed oxidative isomerization of propargylic acetates: highly efficient access to α-acetoxyenones alkenyl Csp-O bond-forming reductive elimination from Pd.

Chem Commun (Camb) 2020 Oct;56(85):13052

School of Chemical Engineering, Shandong University of Technology, 266 West Xincun Road, Zibo 255049, P. R. China. and College of Materials Science and Engineering, Hunan University, Changsha, Hunan 410082, China.

Correction for 'Pd-Catalyzed oxidative isomerization of propargylic acetates: highly efficient access to α-acetoxyenones via alkenyl Csp2-O bond-forming reductive elimination from PdIV' by Jun Li et al., Chem. Commun., 2016, 52, 10644-10647, DOI: 10.1039/C6CC04463H.
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http://dx.doi.org/10.1039/d0cc90452jDOI Listing
October 2020

PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape.

Life Sci 2020 Nov 21;260:118476. Epub 2020 Sep 21.

Hepatobiliary Center, the First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Liver Transplantation, Research Unit of Liver Transplantation and Transplant Immunology, Chinese Academy of Medical Sciences, NHC Key Laboratory of Living Donor Liver Transplantation, Nanjing 210029, China. Electronic address:

Hepatocellular carcinoma (HCC) is the sixth most common malignancy and has the third highest mortality rate among all tumors. Previous studies found that phosphatidylinositol glycan anchor biosynthesis class U (PIGU) was highly expressed in hepatocellular carcinoma (HCC), while the function of PIGU in HCC remains unknown. Here, we deeply investigated this issue. The expression levels of PIGU in HCC cells were measured by Western blotting. The functions of PIGU in HCC cells were assessed in vitro, followed by assessing the nuclear factor-kappa B (NF-κB) pathway-related protein levels. The xenograft mouse models were conducted to investigate the effects of PIGU in vivo. Moreover, the effects of PIGU downregulation on natural killer (NK)-92 cell-mediated cell killing were detected. The results showed that PIGU was highly expressed in HCC cells compared with normal liver cells. Functional studies showed that PIGU promoted viability, cell cycle progression, migration, and invasion and suppressed apoptosis in HCC cells. Mechanism studies indicated that PIGU silencing blocked the NF-κB pathway and the blockade of the NF-κB pathway reversed the effects of PIGU overexpression on HCC cell function, including cell viability, migration, invasion, and apoptosis. In vivo studies further verified the effects of PIGU on HCC cell function, and demonstrated that PIGU knockdown suppressed tumorigenesis. Additionally, we proved that PIGU downregulation significantly enhanced the sensitivity of HCC cells to NK-92 cell cytolysis. Collectively, PIGU may promote HCC progression through activating the NF-κB pathway and promoting immune escape, indicating that PIGU may serve as a promising therapeutic target for HCC treatment.
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http://dx.doi.org/10.1016/j.lfs.2020.118476DOI Listing
November 2020

Can the Wall Shear Stress Values of Left Internal Mammary Artery Grafts during the Perioperative Period Reflect the One-Year Patency?

Thorac Cardiovasc Surg 2020 12 16;68(8):723-729. Epub 2020 Sep 16.

Department of Radiology, Ruijin Hospital affiliated to School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Purpose: The left internal mammary artery (LIMA) is the preferred graft for coronary artery bypass grafting, but the reasoning for LIMA occlusion is unclear. We sought to examine whether the wall shear stress (WSS) values of LIMA grafts during the perioperative period reflected the 1-year patency by using combining computational fluid dynamics (CFD) and coronary computed tomography angiography (CCTA) images.

Methods: CCTA was performed in 233 patients with LIMA graft perioperatively and 1 year later from October 2014 to May 2017. LIMA occlusion was detected in six patients at the 1-year follow-up CCTA. Two patients were excluded due to poor imaging quality. The remaining four patients were enrolled as occlusive (OCC) group, and eight patients with patent LIMA were recruited as patent (PAT) group. The WSS values of LIMA during perioperative period were calculated. LIMA graft was artificially divided into three even segments, proximal (pLIMA), middle (mLIMA) and distal (dLIMA) segments. The independent samples -test and the Student-Newman-Keuls test were used.

Results: The WSS values of dLIMA were significantly higher in the PAT group than in the OCC group (4.43 vs. 2.56,  < 0.05). The WSS values of dLIMA in the PAT group were significantly higher than pLIMA, which was absent in the OCC group.

Conclusions: A higher WSS value of the distal segment of LIMA and a higher WSS value of the distal segment compared with the proximal segment of LIMA in the PAT were observed; this tendency might be helpful in predicting the 1-year patency of LIMA.
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http://dx.doi.org/10.1055/s-0040-1714385DOI Listing
December 2020

6-Gingerol suppresses tumor cell metastasis by increasing YAP phosphorylation in renal cell carcinoma.

J Biochem Mol Toxicol 2021 Jan 14;35(1):e22609. Epub 2020 Sep 14.

Department of Reproductive Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

According to the World Health Organization, the incidence and mortality rates of renal cell carcinoma (RCC) are rapidly increasing worldwide. Serious side effects caused by immune therapy and resistance to targeted drug therapy are urgent clinical problems facing kidney treatment. There is increasing global interest in developing natural products with a reduced number of side effects as adjunctive therapeutic options for RCC. Ginger is a spice and herbal remedy used worldwide, and 6-gingerol is a major pharmacologically active ingredient in ginger. In our study, we found that 6-gingerol suppressed RCC cell migration and metastasis in vitro and in vivo. Moreover, reduction in MMP2, Slug, and Vimentin protein levels was observed following 6-gingerol treatment of 786-O and ACHN cells. Furthermore, we revealed the mechanisms underlying the ability of 6-gingerol to inhibit RCC cell migration and metastasis. 6-Gingerol increased yes-associated protein (YAP) phosphorylation and reduced YAP levels in cell nuclei. We also used a series of loss-of-function and gain-of-function experiments to support our results. Western blot results showed that MMP2, Slug, and Vimentin protein expression was downregulated in YAP-silenced cells and upregulated in YAP-overexpressing cells. Transwell data demonstrated that YAP suppressed RCC migration ability. Immunofluorescence images showed that 6-gingerol decreased YAP levels, leading to disordered F-actin and a reduction in cell lamellipodia. Overall, our results indicated that 6-gingerol is a potential antimetastatic compound for use in kidney therapy.
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http://dx.doi.org/10.1002/jbt.22609DOI Listing
January 2021

The Genome Sequence of Alpine Identifies Species-Specific Whole-Genome Duplication.

Front Genet 2020 3;11:812. Epub 2020 Aug 3.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Sciences, Sichuan University, Chengdu, China.

(Brassicaceae), a plant found the high mountains of southwest China at high altitudes (3000-4800 m), is used as a vegetable or medicine. Here, we report a draft genome for this species. The assembly genome of is 883 Mb, and 61.59% of the genome is composed of repeat sequences. Annotation of the genome identified a total of 41,114 protein-coding genes. We found that experienced an independent whole-genome duplication (WGD), paralleling those independent WGDs in , , and in the early Miocene. Phylogenetic analyses based on the single-copy genes confirmed the position of the genus within the expanded lineage II of the family and resolved its basal divergence to a subclade consisting of , and Species-specific and fast-evolving genes in are mainly involved in "DNA repair" and "response to UV-B radiation." These genetic changes may together help this species survive in high-altitude environments. The reference genome reported here provides a valuable resource for studying adaptation of this and other alpine plants to the high-altitude habitats.
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http://dx.doi.org/10.3389/fgene.2020.00812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416671PMC
August 2020

COVID-19 patients with progressive and non-progressive CT manifestations.

Radiol Infect Dis 2020 Sep 15;7(3):97-105. Epub 2020 Jul 15.

Department of Radiology, Shanghai Jiao Tong University Medical School Affiliated Ruijin Hospital, Shanghai 200025, China.

Objective: To explore the clinical and radiological characteristics of COVID-19 patients with progressive and non-progressive CT manifestations.

Methods: 160 patients with COVID-19 were retrospectively included from Wenzhou and Wuhan, China. CT features including lesion position, attenuation, form and total scores (0-4) at the segment level were evaluated. Other images signs were also assessed. 65 patients were classified as progressive (group 1) and 95 as non-progressive CT (group 2) groups according to score changes between the initial and second CT.

Results: Symptoms onset-initial CT interval time in group 1 [5 (2, 7) days] were significantly shorter than that in group 2 [10 (8, 14) days] ( < 0.001). Group 2 had higher radiological scores, with more lobes and segments affected, and other CT signs ( < 0.05). In group 1, radiological scores, the number of lobes and segments affected as well as lesions in both peripheral and central distribution, mixed ground grass opacity and consolidation density, and patchy form increased in the second CT ( < 0.05). More reticular pattern, subpleural linear opacity and bronchial dilatation were also found ( < 0.05).

Conclusion: Typically radiological characteristics of progressive CT patients could potentially help to predict changes and increase understanding of the natural history of COVID-19.
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http://dx.doi.org/10.1016/j.jrid.2020.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362871PMC
September 2020

Clinical remission of a critically ill COVID-19 patient treated by human umbilical cord mesenchymal stem cells: A case report.

Medicine (Baltimore) 2020 Jul;99(31):e21429

Intervention and Cell Therapy Center, Peking University Shenzhen Hospital, Shenzhen.

Rationale: The COVID-19 cases increased very fast in January and February 2020. The mortality among critically ill patients, especially the elder ones, is relatively high. Considering many patients died of severe inflammation response, it is urgent to develop effective therapeutic strategies for these patients. The human umbilical cord mesenchymal stem cells (hUCMSCs) have shown good capabilities to modulate the immune response and repair the injured tissue. Therefore, investigating the potential of hUCMSCs to the treatment of COVID-19 critically ill patients is necessary.

Patient Concerns: A 65-year-old woman felt fatigued and had a fever with body temperature of 38.2C, coughed up white foaming sputum. After 1 day, she had chest tightness with SPO2 of 81%, and blood pressure of 160/91 mm Hg.

Diagnose: According to the guideline for the diagnosis and treatment of 2019 novel coronavirus infected pneumonia (Trial 4th Edition), COVID-19 was diagnosed, based on the real-time RT-PCR test of SARS-CoV-2.

Interventions: After regular treatment for 12 days, the inflammation symptom of the patient was still very severe and the potential side effects of corticosteroid were observed. Then, allogenic hUCMSCs were given 3 times (5 × 10 cells each time) with a 3-day interval, together with thymosin α1 and antibiotics daily injection.

Outcomes: After these treatments, most of the laboratory indexes and CT images showed remission of the inflammation symptom. The patient was subsequently transferred out of ICU, and the throat swabs test reported negative 4 days later.

Lessons: These results indicated the clinical outcome and good tolerance of allogenic hUCMSCs transfer.
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http://dx.doi.org/10.1097/MD.0000000000021429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7402800PMC
July 2020

Reply to "On the Role of Chest Radiography and CT in the Coronavirus Disease (COVID-19) Pandemic".

AJR Am J Roentgenol 2020 10 22;215(4):W45. Epub 2020 Jul 22.

Shanghai Jiao Tong University, School of Medicine, Shanghai, China

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http://dx.doi.org/10.2214/AJR.20.24159DOI Listing
October 2020

Snail promotes prostate cancer migration by facilitating SPOP ubiquitination and degradation.

Biochem Biophys Res Commun 2020 08 20;529(3):799-804. Epub 2020 Jul 20.

Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, PR China. Electronic address:

Prostate cancer (PCa) is the second leading cause of cancer-associated mortality in men. Speckle-type pox virus and zinc finger protein (SPOP), the most frequently mutated gene in PCa, functions as a tumor suppressor via degradation of cancer-promoting substrates. However, its upstream regulation in PCa metastasis remains poorly determined. Here, in a Snail-induced metastatic PCa model, we observed an accelerated degradation of SPOP protein in cells, which is crucial for the PCa migration and activation of the AKT signaling pathway. Mechanistically, we demonstrated that binding to Snail promoted SPOP ubiquitination and degradation. Moreover, the bric-a-brac/tramtrack/broad complex (BTB) domain of SPOP is turned out to be essential for Snail-mediated SPOP degradation. Thus, our findings reveal a post-translational level regulation of SPOP expression that facilitates the metastasis of PCa cells.
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http://dx.doi.org/10.1016/j.bbrc.2020.05.187DOI Listing
August 2020