Publications by authors named "Wenjie Li"

408 Publications

Discovery and biosynthesis of cyclic plant peptides via autocatalytic cyclases.

Nat Chem Biol 2021 Nov 22. Epub 2021 Nov 22.

Department of Medicinal Chemistry, University of Michigan, Ann Arbor, MI, USA.

Many bioactive plant cyclic peptides form side-chain-derived macrocycles. Lyciumins, cyclic plant peptides with tryptophan macrocyclizations, are ribosomal peptides (RiPPs) originating from repetitive core peptide motifs in precursor peptides with plant-specific BURP (BNM2, USP, RD22 and PG1beta) domains, but the biosynthetic mechanism for their formation has remained unknown. Here, we characterize precursor-peptide BURP domains as copper-dependent autocatalytic peptide cyclases and use a combination of tandem mass spectrometry-based metabolomics and plant genomics to systematically discover five BURP-domain-derived plant RiPP classes, with mono- and bicyclic structures formed via tryptophans and tyrosines, from botanical collections. As BURP-domain cyclases are scaffold-generating enzymes in plant specialized metabolism that are physically connected to their substrates in the same polypeptide, we introduce a bioinformatic method to mine plant genomes for precursor-peptide-encoding genes by detection of repetitive substrate domains and known core peptide features. Our study sets the stage for chemical, biosynthetic and biological exploration of plant RiPP natural products from BURP-domain cyclases.
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http://dx.doi.org/10.1038/s41589-021-00892-6DOI Listing
November 2021

Vitamin D receptor methylation attenuates the association between physical activity and type 2 diabetes mellitus: a case-control study.

J Diabetes 2021 Nov 8. Epub 2021 Nov 8.

College of Public Health, Zhengzhou University, Zhengzhou, China.

Background: Physical activity and vitamin D receptor (VDR) were associated with Type 2 Diabetes Mellitus (T2DM). However, the associations of VDR methylation with T2DM and physical activity remain unknown. We aimed to investigate whether VDR methylation was a link between physical activity and T2DM.

Methods: A 1:1 matching case-control study was designed based on the Henan Rural Cohort Study, including 272 pairs of T2DM patients and non-patients. Physical activity level was assessed using the International Physical Activity Questionnaire. High resolution melt (HRM) method was applied to determine the methylation level of the promoter region of VDR. Association between physical activity and T2DM was analyzed with conditional logistic regression model. The effect modification of VDR methylation levels on the association between physical activity and T2DM was conducted. Multivariate correlation analysis model was applied to investigate correlations of VDR methylation with insulin sensitivity.

Results: Physical activity level was associated with T2DM risk (crude model: OR: 0.611, 95% CI: 0.416-0.897; adjusted model: OR: 0.619, 95% CI: 0.418-0.917). In effect modification analysis, the effects of physical activity on T2DM were stronger for low VDR methylation level than high (P=0.025). Besides, VDR methylation levels was associated with insulin (r=-0.089, P=0.039), as well as HOMA-IR (r=-0.098, P=0.022).

Conclusions: Methylation status of VDR promoter is associated with the secretion and sensitivity of insulin. VDR methylation attenuates the association between physical activity and T2DM, indicating that proactively physical activity may reduce the risk of T2DM, especially in people with low VDR methylation level.
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http://dx.doi.org/10.1111/1753-0407.13239DOI Listing
November 2021

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J Pharmacol Exp Ther 2021 Nov 8. Epub 2021 Nov 8.

Department of Neurosurgery, Qilu Hospital, Institute of Brain and Brain-Inspired Science, China

Thiabendazole (TBZ), approved by the U.S. Food and Drug Administration (FDA) for human oral use, elicits a potential anti-cancer activity on cancer cells and in animal models. Here, we evaluated the efficacy of TBZ in the treatment of human glioblastoma multiforme (GBM). TBZ reduced the viability of GBM cells (P3, U251, LN229, A172, and U118MG) relative to controls in a dose- and time-dependent manner. However, normal human astrocytes (NHA) exhibited a greater IC50 than tumor cells lines and were thus, more resistant to its cytotoxic effects. EdU positive cells and the number of colonies formed was decreased in TBZ-treated cells (at 150 μM, < 0.05 and at 150 μM, < 0.001, respectively). This decrease in proliferation was associated with a G2/M arrest as assessed with flow cytometry, and the downregulation of G2/M check point proteins. In addition, TBZ suppressed GBM cell invasion. Analysis of RNA sequencing data comparing TBZ treated cells with controls yielded a group of differentially expressed genes, the functions of which were associated with the cell cycle and DNA replication. The most significantly downregulated gene in TBZ-treated cells was mini-chromosome maintenance protein 2 (MCM2). SiRNA knockdown of MCM2 inhibited proliferation, causing a G2/M arrest in GBM cell lines and suppressed invasion. Taken together, our results demonstrated that TBZ inhibited proliferation and invasion in GBM cells through targeting of MCM2. TBZ inhibits the proliferation and invasion of glioblastoma cells by downregulating the expression of MCM2. These results support the repurposing of TBZ as a possible therapeutic drug in the treatment of GBM.
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http://dx.doi.org/10.1124/jpet.121.000852DOI Listing
November 2021

Toxic effects of formaldehyde and the protective effect of docosahexaenoic acid in .

Transl Neurosci 2021 Jan 4;12(1):351-361. Epub 2021 Oct 4.

Department of Human Anatomy, Medical College of Soochow University, 199 Ren-Ai Road, Suzhou 215123, PR China.

Formaldehyde (FA) is a commercially important chemical applied in industry and scientific research. However, FA has a distinct impact on learning and memory. Although the mechanisms of FA toxicity have been well studied, additional research is required to establish the mechanisms of neuroprotection in cases of FA exposure. Docosahexaenoic acid (DHA) is a polyunsaturated fatty acid with a variety of health benefits, including the enhancement of learning and memory. In this study, we investigated the neuroprotective effects of DHA in that had ingested FA. Our data suggested that DHA enhanced reproductive processes, leading to an increase in the number of eggs, larvae, and adults. Surprisingly, we found that DHA had a mild protective effect against FA-induced impairments in learning and memory.
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http://dx.doi.org/10.1515/tnsci-2020-0186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491587PMC
January 2021

Identifying Circular RNAs in HepG2 Expressing Genotype IV Swine Hepatitis E Virus ORF3 Via Whole Genome Sequencing.

Cell Transplant 2021 Jan-Dec;30:9636897211055042

College of Veterinary Medicine, Southwest University, Chongqing, China.

Swine hepatitis E (SHE) is a new type of zoonotic infectious disease caused by swine hepatitis E virus (SHEV). Open reading frame 3 (ORF3) is a key regulatory and virulent protein of SHEV. Circular RNAs (circRNAs) are a special kind of non-coding RNA molecule, which has a closed ring structure. In this study, to identify the circRNA profile in host cells affected by SHEV ORF3, adenovirus ADV4-ORF3 mediated the overexpression of ORF3 in HepG2 cells, whole genome sequencing was used to investigate the differentially expressed circRNAs, GO and KEGG were performed to enrichment analyze of differentially expressed circRNA-hosting gene, and Targetscan and miRanda softwares were used to analyze the interaction between circRNA and miRNA. The results showed adenovirus successfully mediated the overexpression of ORF3 in HepG2 cells, 1,105 up-regulation circRNAs and 1,556 down-regulation circRNAs were identified in ADV4-ORF3 infection group compared with the control. GO function enrichment analysis of differentially expressed circRNAs-hosting genes classified three main categories (cellular component, biological process and molecular function). KEGG pathway enrichment analysis scatter plot showed the pathway term of top20. The circRNAs with top10 number of BS sites for qRT-PCR validation were selected to confirmed, the results indicated that the up-regulated hsa_circ_0001423 and hsa_circ_0006404, and down-regulated of hsa_circ_0004833 and hsa_circ_0007444 were consistent with the sequencing data. Our findings first preliminarily found that ORF3 protein may affect triglyceride activation (GO:0006642) and riboflavin metabolism (ko00740) in HepG2 cells, which provides a scientific basis for further elucidating the effect of ORF3 on host lipid metabolism and the mechanism of SHEV infection.
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http://dx.doi.org/10.1177/09636897211055042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552397PMC
October 2021

Conformal High-Power-Density Half-Heusler Thermoelectric Modules: A Pathway toward Practical Power Generators.

ACS Appl Mater Interfaces 2021 Nov 26;13(45):53935-53944. Epub 2021 Oct 26.

Department of Materials Science and Engineering, Pennsylvania State University, University Park, Pennsylvania 16802, United States.

Thermoelectric generators (TEGs) exploiting the Seebeck effect provide a promising solution for waste heat recovery. Among the large number of thermoelectric (TE) materials, half-Heusler (hH) alloys are leading candidates for medium- to high-temperature power generation applications. However, the fundamental challenge in this field has been inhomogeneous material properties at large wafer diameters, insufficient power output from the modules, and rigid form factors of TE modules. This has restricted the transition of TEGs in practical applications for over three decades. Here, we successfully demonstrate large diameter wafers with uniform TE properties, high-power conformal hH TE modules for high-temperature application, and their direct integration on flue gas platforms, such as cylindrical tubes, to form large area flexible TEGs. This new conformal architecture design provides a breakthrough toward medium-/high-temperature TEGs over the conventional BiTe- and polymer-based flexible TEG design. A variable fill factor and greater flexibility due to the conformal design result in higher device performance as compared to conventional rigid TEG devices. Modules with 72-couple hH legs exhibit a device high-power-density of 3.13 W cm and a total output power of 56.6 W under a temperature difference of 570 °C. These results provide a promising pathway toward widespread utilization of thermoelectric technology into the waste heat recovery application and will have a significant impact on the development of practical thermal to electrical converters.
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http://dx.doi.org/10.1021/acsami.1c16117DOI Listing
November 2021

Genomic Epidemiology of SARS-CoV-2 in Pakistan.

Genomics Proteomics Bioinformatics 2021 Oct 22. Epub 2021 Oct 22.

China National Center for Bioinformation, Beijing 100101, China; National Genomics Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

COVID-19 has swept globally and Pakistan is no exception. To investigate the initial introductions and transmissions of the SARS-CoV-2 in Pakistan, we performed the largest genomic epidemiology study of COVID-19 in Pakistan and generated 150 complete SARS-CoV-2 genome sequences from samples collected before June 2, 2020. We identified a total of 347 mutated positions, 31 of which were over-represented in Pakistan. Meanwhile, we found over 1000 intra-host single-nucleotide variants (iSNVs). Several of them occurred concurrently, indicating possible interactions among them or coevolution. Some of the high-frequency iSNVs in Pakistan were not observed in the global population, suggesting strong purifying selections. The genomic epidemiology revealed five distinctive spreading clusters. The largest cluster consisted of 74 viruses which were derived from different geographic locations of Pakistan and formed a deep hierarchical structure, indicating an extensive and persistent nation-wide transmission of the virus that was probably attributed to a signature mutation (G8371T in ORF1ab) of this cluster. Furthermore, 28 putative international introductions were identified, several of which are consistent with the epidemiological investigations. In all, this study has inferred the possible pathways of introduction and transmissions of SARS-CoV-2 in Pakistan, which could aid ongoing and future viral surveillance and COVID-19 control.
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http://dx.doi.org/10.1016/j.gpb.2021.08.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546014PMC
October 2021

The Hippo pathway: a renewed insight in the craniofacial diseases and hard tissue remodeling.

Int J Biol Sci 2021 24;17(14):4060-4072. Epub 2021 Sep 24.

Xiangya School of Stomatology, Central South University, Changsha 410008, China.

The Hippo pathway plays an important role in many pathophysiological processes, including cell proliferation and differentiation, cell death, cell migration and invasion. Because of its extensive functions, Hippo pathway is closely related to not only growth and development, but also many diseases, including inflammation and cancer. In this study, the role of Hippo pathway in craniofacial diseases and hard tissue remodeling was reviewed, in attempting to find new research directions.
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http://dx.doi.org/10.7150/ijbs.63305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495397PMC
September 2021

Abundance, diversity and diffusion of antibiotic resistance genes in cat feces and dog feces.

Environ Pollut 2022 Jan 11;292(Pt A):118364. Epub 2021 Oct 11.

Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

The ARG profiles in pet feces, such as cat and dog feces, and their potential threat to environmental safety are still unclear. In this study, ARGs in 45 cat and 28 dog fecal samples were detected, and a diffusion experiment was performed to assess the risk of ARGs diffusion into the air. The results showed that the abundances of ARGs in cat feces and dog feces were high, and the abundance in dog feces (0.89 ± 0.17 copies/bacterial cell) was significantly higher than that in cat feces (0.46 ± 0.09 copies/bacterial cell) (P < 0.05). The bacterial community, especially Firmicutes and Desulfobacterota in cat feces, and Proteobacteria in dog feces, was the main factor affecting the variation in the ARG profiles, contributing to 31.6% and 32.4% of the variation in cat feces and dog feces, respectively. Physicochemical factors (especially NH-N) and age also indirectly affected the variation in the ARG profiles by affecting the bacterial community. In addition, the ARGs in cat feces and dog feces diffused into the air, but there was no evidence that this diffusion posed a threat to environmental safety and human health. These results can provide reference data for healthy animal breeding and the prevention and control of ARG pollution.
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http://dx.doi.org/10.1016/j.envpol.2021.118364DOI Listing
January 2022

Homography-based robust pose compensation and fusion imaging for augmented reality based endoscopic navigation system.

Comput Biol Med 2021 11 15;138:104864. Epub 2021 Sep 15.

Beijing Engineering Research Center of Mixed Reality and Advanced Display, School of Optics and Photonics, Beijing Institute of Technology, Beijing, 100081, China.

Background: Augmented reality (AR) based fusion imaging in endoscopic surgeries rely on the quality of image-to-patient registration and camera calibration, and these two offline steps are usually performed independently to get the target transformation separately. The optimal solution can be obtained under independent conditions but may not be globally optimal. All residual errors will be accumulated and eventually lead to inaccurate AR fusion.

Methods: After a careful analysis of the principle of AR imaging, a robust online calibration framework was proposed for an endoscopic camera to enable accurate AR fusion. A 2D checkerboard-based homography estimation algorithm was proposed to estimate the local pose of the endoscopic camera, and the least square method was used to calculate the compensation matrix in combination with the optical tracking system.

Results: In comparison with conventional methods, the proposed compensation method improved the performance of AR fusion, which reduced physical error by up to 82%, reduced pixel error by up to 83%, and improved target coverage by up to 6%. Experimental results of simulating mechanical noise revealed that the proposed compensation method effectively corrected the fusion errors caused by the rotation of the endoscopic tube without recalibrating the camera. Furthermore, the simulation results revealed the robustness of the proposed compensation method to noises.

Conclusions: Overall, the experiment results proved the effectiveness of the proposed compensation method and online calibration framework, and revealed a considerable potential in clinical practice.
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http://dx.doi.org/10.1016/j.compbiomed.2021.104864DOI Listing
November 2021

Critical Current Density and Vortex Dynamics in Pristine and Irradiated KCaFeAsF.

Materials (Basel) 2021 Sep 14;14(18). Epub 2021 Sep 14.

Department of Applied Physics, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

We report the critical current density () and vortex pinning properties in single crystals of a novel iron-based superconductor (IBS) KCaFeAsF with large in the pristine state, before and after introduction of artificial defects by swift-particle irradiation. The effects of 2.6 GeV U and 3 MeV proton irradiations in KCaFeAsF single crystals on transition temperature and , including its dose dependence, are systematically studied. ~8 MA/cm under a self-field at 2 K in the pristine crystal is strongly enhanced up to 19.4 and 17.5 MA/cm by irradiation of 2.6 GeV U-ions and 3 MeV protons, respectively. Suppression of and dose dependence of in KCaFeAsF is different from that in a representative IBS of (Ba,K)FeAs, which can be explained by considering the presence of embedded defects in pristine KCaFeAsF. The vortex dynamics in the pristine and proton irradiated KCaFeAsF single crystals are also investigated from the analyses of the field dependence of and the normalized magnetic relaxation rate. In addition to the contribution of embedded defects, weak collective pinning is considered for comprehensive analyses. Vortex dynamics in KCaFeAsF is similar to those in (Ba,K)FeAs to some extent, and different from that in anisotropic LiFeOHFeSe. Large anisotropy, due to the presence of insulating blocking layers in KCaFeAsF, which leads to much lower irreversibility field () compared with 122-type IBSs, strongly affect the vortex dynamics.
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http://dx.doi.org/10.3390/ma14185283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465300PMC
September 2021

Combined effects of progesterone and SOCS3 DNA methylation on T2DM: a case-control study.

Clin Epigenetics 2021 09 26;13(1):181. Epub 2021 Sep 26.

Department of Occupational and Environmental Health Sciences, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, Henan, People's Republic of China.

Background: This study aims to investigate the independent and combined effects of progesterone and suppressor of cytokine signaling (SOCS)-3 DNA methylation on type 2 diabetes mellitus (T2DM) among men and postmenopausal women in rural China.

Methods: A case-control study with 914 participants (329 T2DM, 585 controls) was conducted. Serum progesterone was detected with liquid chromatography-tandem mass spectrometry. DNA methylation of SOCS3 was determined by MethylTarget™. Linear regression was applied to evaluate the associations of progesterone and SOCS3 methylation with marks of glucose metabolism. Logistic regression was employed to investigate the independent and combined effects of progesterone and SOCS3 methylation with T2DM in men and postmenopausal women.

Results: After multiple adjustment, progesterone was positively associated with T2DM in both men (odds ratio (OR) (95% confidence interval (CI)): 2.77 (1.79, 4.29)) and postmenopausal women (OR (95% CI): 1.85 (1.26, 2.72)). Methylation level of Chr17:76,356,190 or Chr17:76,356,199 (SOCS3) was negatively associated with T2DM in both men (OR (95% CI): 0.58 (0.39, 0.86) or 0.27 (0.14, 0.51)) and postmenopausal women (OR (95% CI): 0.43 (0.29, 0.65) or 0.53 (0.28, 0.99)). Subjects with high progesterone and low Chr17:76,356,190 or Chr17:76,356,199 methylation were more susceptible to have a higher prevalence of T2DM (men: OR (95% CI): 5.20 (2.49, 10.85) or 5.62 (2.74, 11.54); postmenopausal women: OR (95% CI): 3.66 (1.85, 7.26) or 3.27 (1.66, 6.45)).

Conclusions: The independent and combined effects of progesterone and SOCS3 methylation on T2DM were found among men and postmenopausal women, suggesting that ensuring low levels of progesterone and high methylation of SOCS3 could reduce the prevalence of T2DM. Trial registration The Chinese Clinical Trial registration: The Henan Rural Cohort Study, ChiCTR-OOC-15006699. Registered 06 July 2015, http://www.chictr.org.cn/showproj.aspx?proj=11375.
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http://dx.doi.org/10.1186/s13148-021-01172-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474856PMC
September 2021

Ubiquilin 1 suppresses the cancer stem cell-like traits of non-small cell lung cancer cells by regulating reactive oxygen species homeostasis.

Bioengineered 2021 12;12(1):7143-7155

Department of Geriatric Medicine, Ningbo First Hospital, Ningbo, China.

Cancer stem cell (CSC) has been confirmed to trigger tumor occurrence and progression and CSC can develop strategies to maintain a lower reactive oxygen species (ROS) level compared to cancer cells. However, the mechanisms contributing to ROS homeostasis in CSC are still lacking key elements. In the current study, we found that reductive redox states and ROS levels were suppressed in non-adherent spheres formed by non-small cell lung cancer (NSCLC) cells, which were confirmed to hold CSC-like traits. However, mitochondria DNA content and cellular oxygen consumption rate analyses revealed fewer numbers of mitochondria in NSCLC spheres. Further exploration attributed this result to decreased mitochondrial biogenesis, likely resulted from the accelerated degradation of peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α). Mechanistic studies indicated that Ubiquilin 1 (UBQLN1) increased PGC1α protein stability via reducing the ubiquitination of PGC1α protein. Moreover, UBQLN1 was lowly expressed in NSCLC spheres compared to that in parental NSCLC cells and UBQLN1 overexpression suppressed the CSC-like traits of NSCLC cells, which was characterized as the decrease of ALDH1 activity, sphere-formation ability, and CSC marker expression. Finally, clinical investigations further demonstrated that UBQLN1 level was positively correlated with patient's survival of lung adenocarcinoma, but not squamous cell carcinoma of lung. Taken together, our results revealed a novel mechanism involving ROS homeostasis and mitochondrial biogenesis in non-small cell lung CSCs, which may provide novel potential targets and methods for NSCLC patients.
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http://dx.doi.org/10.1080/21655979.2021.1979353DOI Listing
December 2021

Effect of growth hormone on thin endometrium via intrauterine infusion.

Ann Transl Med 2021 Aug;9(16):1325

Center of Reproductive Medicine, the First Affiliated Yijishan Hospital of Wannan Medical College, Wuhu, China.

Background: A thin endometrium has become a common reason for the repeated implantation failure. Growth hormone (GH) can regulate the proliferation and metabolism of endometrial cells. The aim of this study was to explore the effect of GH on thin endometrium.

Methods: A total of 48 female Sprague-Dawley (SD) rats were randomly assigned to the following 4 groups with 12 rats in each group: blank control, model, subcutaneous, and GH groups. The blank control group was untreated and maintained in a routine manner. The model, subcutaneous, and GH groups were intrauterine perfused with 95% ethanol during estrus. After 6-8 h, the model group was intrauterine perfused with 0.2 mL normal saline, the subcutaneous group received subcutaneous injection of 0.12 mg/kg GH dissolved in 0.2 mL normal saline, and the GH group was intrauterine perfused with 0.12 mg/kg GH dissolved in 0.2 mL normal saline. Hematoxylin and eosin (HE) staining was used to examine the thickness of the endometrium. The expression of cytokeratin and vimentin was detected by western blotting and immunohistochemistry.

Results: The intima thickness in the GH group and blank control group was increased compared with that in the model group (P<0.01). The intima thickness in the subcutaneous group was increased compared with that in the model group, but there was no significant difference. The expression of vimentin and cytokeratin was increased in the GH (P<0.01) and blank control (P<0.01) groups compared with that in the model group.

Conclusions: Intrauterine perfusion of GH can promote the regeneration and repair of thin endometrium in rats. The therapeutic effect of uterine infusion of GH is better than that of subcutaneous injection of GH.
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http://dx.doi.org/10.21037/atm-21-3583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422125PMC
August 2021

Gratitude and depressive symptoms in Chinese nurses: A longitudinal cross-lagged study.

Appl Psychol Health Well Being 2021 Sep 8. Epub 2021 Sep 8.

School of Psychology, Shaanxi Normal University, Xi'an, China.

Nurses have a relatively higher rate of depressive symptoms compared to other people. Previous studies have reported that gratitude is correlated with depressive symptoms among healthcare workers. The purpose of this study was to explore the causal relationship between gratitude and depressive symptoms among Chinese nurses. We collected data over 4 months, with 393 nurses at Time 1 and 241 at Time 2. All participants completed online questionnaires. We employed the structural equation models to test the hypotheses. The results revealed that gratitude at Time 1 could predict depressive symptoms at Time 2, and depressive symptoms at Time 1 could predict gratitude at Time 2. These results remained significant after controlling for gender, age, education, marital status, professional title, general positive affect, and negative affect. The findings provide initial evidence about the causal relationship between gratitude and depressive symptoms among Chinese nurses. Hospital administrators can intervene in depressive symptoms of nurses by developing gratitude interventions. Furthermore, nurses with lower levels of depressive symptoms feel more grateful.
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http://dx.doi.org/10.1111/aphw.12305DOI Listing
September 2021

Newborn screening with targeted sequencing: a multicenter investigation and a pilot clinical study in China.

J Genet Genomics 2021 Aug 30. Epub 2021 Aug 30.

Beijing Key Laboratory for Genetics of Birth Defects, Beijing Pediatric Research Institute, Beijing 100045, China; MOE Key Laboratory of Major Diseases in Children, Beijing 100045, China; Rare Disease Center, National Center for Children's Health, Beijing 100045, China; Beijing Children's Hospital, Capital Medical University, Beijing 100045, China. Electronic address:

Different newborn screening (NBS) programs have been practiced in many countries since the 1960s. It is of considerable interest whether next-generation sequencing is applicable in NBS. We have developed a panel of 465 causative genes for 596 early-onset, relatively high incidence, and potentially actionable severe inherited diseases in our Newborn Screening with Targeted Sequencing (NESTS) program to screen 11,484 babies in 8 Women and Children's hospitals nationwide in China retrospectively. The positive rate from preliminary screening of NESTS was 7.85% (902/11,484). With 45.89% (414/902) follow-up of preliminary positive cases, the overall clinically confirmative diagnosis rate of monogenic disorders was 12.07% (50/414), estimating an average of 0.95% (7.85% × 12.07%) clinical diagnosis rate, suggesting that monogenic disorders account for a considerable proportion of birth defects. The disease/gene spectrum varied in different regions of China. NESTS was implemented in a hospital by screening 3923 newborns to evaluate its clinical application. The turn-around time of a primary report, including the sequencing period of < 7 days, was within 11 days by our automatic interpretation pipeline. Our results suggest that NESTS is feasible and cost-effective as a first-tier NBS program, which will change the status of current clinical practice of NBS in China.
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http://dx.doi.org/10.1016/j.jgg.2021.08.008DOI Listing
August 2021

Comparative efficacy of non-pharmacological interventions on behavioural and psychological symptoms in elders with dementia: A network meta-analysis.

Nurs Open 2021 11 2;8(6):2922-2931. Epub 2021 Sep 2.

Innovation Center of Nursing Research, West China School of Medicine/West China Hospital, Sichuan University, Chengdu, China.

Aim: To explore the best non-pharmaceutical interventions for improving the behavioural and psychological symptoms in elders with dementia.

Design: Bayesian network meta-analysis.

Methods: A comprehensive electronic literature search was performed in five English databases and four Chinese databases to identify relevant randomized controlled trials (RCTs) that were published up to 31 October 2019.

Results: A total of 41 RCTs were included in this network meta-analysis involving 5 different non-pharmacological interventions: therapeutic recreation (TC), reminiscence therapy (RT), behaviour therapy (BT), massage therapy (MT) and individualized nursing (IN).The results of network meta-analysis showed that individualized nursing was the best in improving depression, cognitive function, and activities of daily living of behavioural and psychological symptoms of dementia. Behaviour therapy was the best in improving anxiety symptoms. Reminiscence therapy was the best in improving neuropsychiatric symptoms.
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http://dx.doi.org/10.1002/nop2.1049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510770PMC
November 2021

Design and Development of an Intelligent Skipping Rope and Service System for Pupils.

Healthcare (Basel) 2021 Jul 29;9(8). Epub 2021 Jul 29.

School of Design and Technology, London College of Fashion, University of the Arts London, London WC1V 7EY, UK.

Regular physical activity (PA) contributes to health, growth and development in childhood and it is essential for children to achieve appropriate PA levels (PAL). However, most children around the world fail to comply with the recommended PAL requirements. Rope skipping, as a highly accessible, enjoyable, and affordable physical activity for students, has been considered a sustainable afterschool physical activity to promote physical fitness of students by educators. The booming development of smart fitness product design and the advent of exergames have brought new possibilities for physical education and rope skipping: personalized guidance, intuitive and interesting feedback and visualized exercise data analysis-there is much room for optimization. In this study, an intelligent skipping rope and its service system were studied for primary school students (aged 7-12) who started to get involved in this sport. First, user needs, product functions, and system requirement were summarized by conducting observations and user interviews. Then, a prototype of the hardware and software interface were designed based on analysis of user research. Next, a usability test of the interactive prototype was carried out and optimization was finally made based on the feedback of the usability evaluation. The final system design includes combined innovations in software and hardware with the intention to increase children's participation in physical activity and assist them in skipping rope in the right way with proper equipment and programs.
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http://dx.doi.org/10.3390/healthcare9080954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394005PMC
July 2021

Customized eye modeling for optical quality assessment in myopic femto-LASIK surgery.

Sci Rep 2021 08 6;11(1):16049. Epub 2021 Aug 6.

School of Physics and Optoelectronic Engineering, Foshan University, Foshan, 528000, Guangdong, China.

Refractive surgery is recognized as an effective method for myopia treatment, but it can induce night vision disturbances such as glare. We present an eye modeling method for the optical quality assessment in response to the structural changes in the eyes by femto-LASIK surgery. Customized eye models were built from the measurements of 134 right eyes pre- and post-operatively. Optical performance was evaluated using spot diagrams, point spread functions (PSFs), modulation transfer functions (MTFs), and chromatic aberrations at various fields (0°-30°), different pupil diameters (2-6 mm), and initial myopias (- 1.25 to - 10.5 D). Pupil size and initial myopia are the two major factors that affect visual performance of post-operative eyes. The results of spot diagrams, PSFs, and MTFs indicated that post-operative visual performance deteriorated as the visual field and pupil size increased, and it was significantly influenced by initial myopia. Post-operative chromatic aberrations were also affected by initial myopia. As pupil size increased, the post-operative longitudinal chromatic aberrations tended to decrease slightly, while the transverse chromatic aberrations remained similar. The use of eye modeling for refractive surgery assessment could possibly provide a more personalized surgical approach, could improve the prediction accuracy of refractive surgery outcomes, and promote the invention and development of better surgical methods.
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http://dx.doi.org/10.1038/s41598-021-95730-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346559PMC
August 2021

Hydrogen sulfide and calcium effects on cadmium removal and resistance in the white-rot fungus Phanerochaete chrysosporium.

Appl Microbiol Biotechnol 2021 Aug 6;105(16-17):6451-6462. Epub 2021 Aug 6.

College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.

Hydrogen sulfide (HS), an emerging gas transmitter, has been shown to be involved in multiple intracellular physiological and biochemical processes. In this study, the effects of hydrogen sulfide coupled with calcium on cadmium removal and resistance in Phanerochaete chrysosporium were examined. The results revealed that HS enhanced the uptake of calcium by P. chrysosporium to resist cadmium stress. The removal and accumulation of cadmium by the mycelium was reduced by HS and Ca pretreatment. Moreover, oxidative damage and membrane integrity were alleviated by HS and Ca. Corresponding antioxidative enzyme activities and glutathione were also found to positively respond to HS and Ca, which played an important role in the resistance to cadmium-induced oxidative stress. The effects of hydroxylamine (HA; a hydrogen sulfide inhibitor) and ethylene glycol-bis-(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA; a calcium chelator) toward HS and Ca and their cross-interactions confirmed the positive roles and the potential crosstalk of HS and Ca in cadmium stress resistance. These findings imply that the protective effects of HS in P. chrysosporium under cadmium stress may occur through a reduction in the accumulation of cadmium and promotion of the antioxidant system, and the HS-regulated pathway may be associated with the intracellular calcium signaling system.Key points• Altered monoterpenoid tolerance mainly related to altered activity of efflux pumps.• Increased tolerance to geranic acid surprisingly caused by decreased export activity.• Reduction of export activity can be beneficial for biotechnological conversions.
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http://dx.doi.org/10.1007/s00253-021-11461-1DOI Listing
August 2021

Electrocaloric Performance of Multilayer Ceramic Chips: Effect of Geometric Structure Induced Internal Stress.

ACS Appl Mater Interfaces 2021 Aug 5;13(32):38508-38516. Epub 2021 Aug 5.

Department of Materials Science and Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.

Driven by an ever-growing demand for environmentally benign cooling systems, the past decade has witnessed the booming development in the field of electrocaloric (EC) cooling technology, which is considered as a promising solid-state cooling approach. Multilayer ceramic chip capacitors (MLCCs) represent the optimum structure for EC cooling elements because of large breakdown strengths, low driving voltages, and high macroscopic volumes of active EC materials. However, fundamental relationships between the geometric parameters of MLCCs and the EC coefficient are less understood. In this study, 0.92Pb(MgNb)O-0.08PbTiO (PMN-PT) MLCCs with controlled configurations, such as active/inactive layer thickness, number of layers, and active volume ratio, were fabricated, and their EC performance was evaluated. The electric properties of the MLCCs are confirmed to be closely related to the geometric structure, which influences not only the heat flow but also the internal stress, resulting in the variability of EC performance and reliability/breakdown strength. The internal stress arises due to the residual thermal stress originating from the densification-related shrinkage, thermal expansion mismatch during the sintering, and clamping stress arising from the inactive area due to the large strain from the active area under a high electric field. The geometric structure-based stress distribution and the magnitude of stress on the active layers in MLCCs were determined by finite element modeling (FEM) and correlated with the experimental EC coefficients. The results reveal that a low inactive volume percentage is beneficial toward increasing the breakdown field and enhancement of EC performance because of reduced clamping stress on active EC material.
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http://dx.doi.org/10.1021/acsami.1c09911DOI Listing
August 2021

Plastic and low-cost axial zero thermal expansion alloy by a natural dual-phase composite.

Nat Commun 2021 Aug 4;12(1):4701. Epub 2021 Aug 4.

Beijing Advanced Innovation Center for Materials Genome Engineering, Institute of Solid State Chemistry, Department of Physical Chemistry, University of Science and Technology Beijing, Beijing, China.

Zero thermal expansion (ZTE) alloys possess unique dimensional stability, high thermal and electrical conductivities. Their practical application under heat and stress is however limited by their inherent brittleness because ZTE and plasticity are generally exclusive in a single-phase material. Besides, the performance of ZTE alloys is highly sensitive to change of compositions, so conventional synthesis methods such as alloying or the design of multiphase to improve its thermal and mechanical properties are usually inapplicable. In this study, by adopting a one-step eutectic reaction method, we overcome this challenge. A natural dual-phase composite with ZTE and plasticity was synthesized by melting 4 atom% holmium with pure iron. The dual-phase alloy shows moderate plasticity and strength, axial zero thermal expansion, and stable thermal cycling performance as well as low cost. By using synchrotron X-ray diffraction, in-situ neutron diffraction and microscopy, the critical mechanism of dual-phase synergy on both thermal expansion regulation and mechanical property enhancement is revealed. These results demonstrate that eutectic reaction is likely to be a universal and effective method for the design of high-performance intermetallic-compound-based ZTE alloys.
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http://dx.doi.org/10.1038/s41467-021-25036-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8338949PMC
August 2021

The expression of constitutively active CPK3 impairs potassium uptake and transport in Arabidopsis under low K stress.

Cell Calcium 2021 09 19;98:102447. Epub 2021 Jul 19.

State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, Zhejiang 311300, China. Electronic address:

Potassium (K) is a vital cation and is involved in multiple physiological functions in plants. K uptake from outer medium by roots is a tightly regulated process and is mainly carried out by two high affinity K transport proteins AKT1 and HAK5. It has been shown that calcium (Ca) signaling plays important roles in the regulation of K transport in plants. Ca-dependent protein kinases (CPKs) are involved in regulation of multiple K channels in different tissues. However, it remains to be studied whether CPKs are involved in the regulation of AKT1 and, thereby, K transport. Here, we have shown that constitutively active version of CPK3 (CPK3CA) is involved in K transport in Arabidopsis via regulating AKT1 under low K conditions. The constitutively active version of CPK3 (CPK3CA), as well as CPK21 (CPK21CA), inhibited K currents of AKT1 in Xenopus oocytes. CPK3CA inhibited only channel conductance but had no effect on channel open probability. Further, CPK3 in vivo interacted with AKT1. Under low K conditions, cpk3 knock-out mutants had no distinct phenotype, while the seedlings of 35S-CPK3CA overexpressing lines died even at normal K concentration. Further, the transgenic lines expressing CPK3CA under AKT1 promoter (Pro-CPK3CA) exhibited the same phenotype as akt1 mutant with a defective root growth and leaf chlorosis. Moreover, Pro-CPK3CA transgenic lines had lower root and shoot K contents than Col. Overall, the data reported here demonstrate that the expression of constitutively active of CPK3 impairs potassium uptake and transports in Arabidopsis under low K stress by inhibiting the activity of AKT1.
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http://dx.doi.org/10.1016/j.ceca.2021.102447DOI Listing
September 2021

Electrochemical removal of NO by LaSrMnNiO electrodes in solid electrolyte cells: Role of Ni substitution.

J Hazard Mater 2021 10 14;420:126640. Epub 2021 Jul 14.

College of Environmental Science and Engineering, Nankai University, Tianjin 300350, PR China. Electronic address:

Electrochemical removal of nitrogen oxides (NO) by solid electrolyte cells (SECs) is a promising technology due to no required reductant. Herein, a series of LaSrMnNiO (0 ≤ x ≤ 0.5) perovskites were first synthesized and utilized as the electrode materials of SECs. The role of Ni substitution in electrode performance and NO reduction mechanism were revealed by various experimental characterization and first-principle calculations. The results indicate that the moderate Ni substitution (x ≤ 0.3) increased the NO conversion of electrodes while reduced the polarization resistance. The further investigation shows that this improvement was attributed to the more surface oxygen vacancies, better reducibility and higher Mn proportion of the Ni-substituted perovskites. The electrochemical impedance spectroscopy (EIS) shows that these changes facilitated the NO adsorption and dissociation processes on the electrode. According to first-principle calculations, the Ni-substituted perovskite had a lower formation energy of surface oxygen vacancy, while the NO molecule adsorbed on defect surface gained more electrons thus was easier to be reduced and dissociated. Finally, the electrode performance at different operating temperatures and the operational stability were verified.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126640DOI Listing
October 2021

Heritability Estimation and Environmental Risk Assessment for Type 2 Diabetes Mellitus in a Rural Region in Henan, China: Family-Based and Case-Control Studies.

Front Public Health 2021 8;9:690889. Epub 2021 Jul 8.

Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou, China.

The prevalence of type 2 diabetes mellitus (T2DM) varies greatly in different regions and populations. This study aims to assess the heritability and environmental risk factors of T2DM among rural Chinese adults. Thousand five hundred thirty three participants from 499 extended families, which included 24 nuclear families, were recruited in the family-based study to assess the heritable risk of T2DM. Heritability of T2DM was estimated by the Falconer method. Using conditional logistic regression model, couple case-control study involving 127 couples were applied to assess the environmental risk factors of T2DM. Compared with the Henan Rural Cohort, T2DM was significantly clustered in the nuclear families (OR: 8.389, 95% CI: 5.537-12.711, < 0.001) and heritability was 0.74. No association between the heredity of T2DM and sex was observed between the extended families and the Henan Rural Cohort. Besides, results from the couple case-control study showed that physical activity (OR: 0.482, 95% CI: 0.261-0.893, = 0.020) and fat intake (OR: 3.036, 95% CI: 1.070-8.610, = 0.037) was associated with T2DM, and the proportion of offspring engaged in medium and high physical activity was higher than that of mothers in mother-offspring pairs. People with a family history of T2DM may have a higher risk of developing T2DM, however, there was no difference in genetic risk between males and females. Adherence to active physical activity and low fat intake can reduce the risk of T2DM.
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http://dx.doi.org/10.3389/fpubh.2021.690889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295650PMC
August 2021

States Transitions Inference of Postpartum Depression Based on Multi-State Markov Model.

Int J Environ Res Public Health 2021 07 13;18(14). Epub 2021 Jul 13.

Health Science Center, Shenzhen University, Shenzhen 518060, China.

: Postpartum depression (PPD) has been recognized as a severe public health problem worldwide due to its high incidence and the detrimental consequences not only for the mother but for the infant and the family. However, the pattern of natural transition trajectories of PPD has rarely been explored. In this research, a quantitative longitudinal study was conducted to explore the PPD progression process, providing information on the transition probability, hazard ratio, and the mean sojourn time in the three postnatal mental states, namely normal state, mild PPD, and severe PPD. The multi-state Markov model was built based on 912 depression status assessments in 304 Chinese primiparous women over multiple time points of six weeks postpartum, three months postpartum, and six months postpartum. : Among the 608 PPD status transitions from one visit to the next visit, 6.2% (38/608) showed deterioration of mental status from the level at the previous visit; while 40.0% (243/608) showed improvement at the next visit. A subject in normal state who does transition then has a probability of 49.8% of worsening to mild PPD, and 50.2% to severe PPD. A subject with mild PPD who does transition has a 20.0% chance of worsening to severe PPD. A subject with severe PPD is more likely to improve to mild PPD than developing to the normal state. On average, the sojourn time in the normal state, mild PPD, and severe PPD was 64.12, 6.29, and 9.37 weeks, respectively. Women in normal state had 6.0%, 8.5%, 8.7%, and 8.8% chances of progress to severe PPD within three months, nine months, one year, and three years, respectively. Increased all kinds of supports were associated with decreased risk of deterioration from normal state to severe PPD (hazard ratio, HR: 0.42-0.65); and increased informational supports, evaluation of support, and maternal age were associated with alleviation from severe PPD to normal state (HR: 1.46-2.27). The PPD state transition probabilities caused more attention and awareness about the regular PPD screening for postnatal women and the timely intervention for women with mild or severe PPD. The preventive actions on PPD should be conducted at the early stages, and three yearly; at least one yearly screening is strongly recommended. Emotional support, material support, informational support, and evaluation of support had significant positive associations with the prevention of PPD progression transitions. The derived transition probabilities and sojourn time can serve as an importance reference for health professionals to make proactive plans and target interventions for PPD.
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http://dx.doi.org/10.3390/ijerph18147449DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304364PMC
July 2021

In vitro and in vivo antioxidant activities of soy protein isolate fermented with natto.

J Food Sci Technol 2021 Aug 30;58(8):3199-3204. Epub 2020 Sep 30.

College of Food Science, Shenyang Agricultural University, Shenyang, 110866 China.

The natto fermented soy protein isolate (FSPI) exhibited concentration-dependent scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS+·) and hydroxyl (·OH) free-radicals. In addition, FSPI administration significantly increased superoxide dismutase activity in mice liver and serum by 20.2% and 86.2%, and suppressed the production of malondialdehyde by 51.3% and 35.1%, respectively, compared to high-fat control (HFC) group. Notably, the movement of mice treated with FSPI was livelier and more active, and its weight gain was significantly lower than that of both NC and HFC groups. The production and accumulation of perirenal fat was also significantly inhibited by FSPI, however, no significant difference in TG and TC levels were observed between FSPI and HFC groups. The results revealed the great potential of FSPI applying in the development of health food or sports food.
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http://dx.doi.org/10.1007/s13197-020-04823-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249477PMC
August 2021

Endoscopic closure of tracheoesophageal fistula with a novel dumbbell-shaped occluder.

Endoscopy 2021 Jul 19. Epub 2021 Jul 19.

Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

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http://dx.doi.org/10.1055/a-1524-0761DOI Listing
July 2021

Changes in radiological parameters during reduction of femoral neck fractures: A radiographic evaluation of cadavers.

Injury 2021 Oct 10;52(10):2827-2834. Epub 2021 Jul 10.

Department of Orthopaedics Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, NO.109, XueYuan West Road, Luheng District, Wenzhou, Zhejiang Province, 325000, PR China. Electronic address:

Objective: To investigate changes in the Garden index and other radiological parameters during reduction of femoral neck fractures.

Methods: Ten healthy, human femoral specimens were obtained. A 2.0 mm diameter Kirschner wire was implanted in the centre of the femoral head. A perpendicular osteotomy was made in the middle of the femoral neck. The distal osteotomy surface was used as the angle of rotation (pronation and supination up to 90° at 10° intervals). Anterior-posterior and lateral view radiographs were taken at different angles. The Garden index and other relevant data were analysed using the picture archiving and communication system. Changes in the area of the femoral head fovea at different rotation angles were measured.

Results: There were no significant differences in the Garden index between 0-30° of pronation and supination (p > .05). For angles of 40-90°, there were statistically significant differences in the Garden index (p < .05). The area of femoral head fovea decreased with increasing pronation angle, and increased with increasing supination angle.

Conclusions: The Garden index does not change significantly if the angle of fracture rotation is 0-30° (in either pronation or supination) during femoral neck fracture reduction. Therefore, it is impossible to judge the rotation of fracture in this range of angles. The Garden index can detect the rotation of fracture for rotation angles of 40-90° (in either pronation or supination). Changes in the area of the femoral head fovea can help determine the rotation of femoral neck fractures.

Level Of Evidence: Level V.
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http://dx.doi.org/10.1016/j.injury.2021.07.011DOI Listing
October 2021
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