Publications by authors named "Wenhui Tian"

3 Publications

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Protective effect against d-gal-induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion.

J Food Sci 2021 Jun 7;86(6):2736-2752. Epub 2021 May 7.

Institute of Agriculture and Food, Kunming University of Science and Technology, Kunming, Yunnan, P. R. China.

Defatted walnut kernel with pellicle (WKP) is an industrial byproduct during walnut oil extraction, which is rich in protein and polyphenols. WKP was hydrolyzed by simulated gastrointestinal digestion to obtain WKP hydrolysates (WKPHs). Results showed the protein recovery and hydrolysis degree of WKPH were 82.15 and 10.36%. The total phenol contents in WKP and WKPH were 4.90 and 40.70 mg gallic acid equivalent/g, respectively. The antiaging activity of WKPH was evaluated using a d-gal-induced aging mouse model. Results showed that WKPHs could recover the activities of SOD and T-AOC and the content of MDA in tissues and serum of the aging mice. The histological morphology of liver and kidney sections and the immunohistochemistry of TNF-α, IL-1β, and IL-6 in liver were observed. WKPH could effectively protect the tissue structure of the liver and kidney and reduce the inflammatory expression of liver in aging mice. The polypeptides and polyphenols in WKPH were further analyzed. Fifty polypeptides were identified and 12 of these peptides had Leu-Arg at the C-terminal. Forty-two polyphenols were detected, and most phenolic compounds belonged to ellagitannins. This study provided a theoretical basis for the improved processing and high-value utilization of walnut byproducts. PRACTICAL APPLICATION: Defatted walnut kernel with pellicle was hydrolyzed by simulated gastrointestinal digestion to obtain its hydrolysates. The hydrolysates have good antiaging activity in vivo. Fifty polypeptides were identified and 12 of these peptides had Leu-Arg at the C-terminal. Forty-two polyphenols were detected, and most phenolic compounds belonged to ellagitannins. This study could provide a theoretical basis for high-value utilization of walnut byproducts.
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http://dx.doi.org/10.1111/1750-3841.15744DOI Listing
June 2021

In vivo acute and subacute toxicities of phenolic extract from rambutan (Nephelium lappaceum) peels by oral administration.

Food Chem 2020 Aug 15;320:126618. Epub 2020 Mar 15.

Faculty of Agriculture and Food, Kunming University of Science and Technology, No. 727 South Jingming Road, Kunming, Yunnan 650500, China. Electronic address:

Acute and subacute studies of rambutan peel phenolic (RPP) extract were conducted by oral administration on Kunming mice and Sprague-Dawley (SD) rats, respectively. Acute toxicity study (14 days) results revealed that the LD value of RPP extract was more than 5000 mg/kg bw in vivo. For the subacute study, four different doses were administered to SD rats by daily gavage for 28 days. Subacute toxicity study results indicated that RPP extract did not show any obvious adverse effect at doses of 312 and 625 mg/kg bw. The bw gain was significantly inhibited at 2500 mg/kg bw of RPP extract. RPP extract at doses of 1250 and 2500 mg/kg bw showed toxicities to liver, kidney, and spleen in SD rats according to the results of hematological and biochemical analyses. Furthermore, RPP extract at 2500 mg/kg bw showed toxicity on different tissues according to the results of histopathological analyses.
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http://dx.doi.org/10.1016/j.foodchem.2020.126618DOI Listing
August 2020

Transcriptome sequencing of from a cotton farm reveals positive correlation between virulence and tolerance of sugar-induced hyperosmosis.

PeerJ 2019 11;7:e8035. Epub 2019 Nov 11.

College of Life Science, Shihezi University, Shihezi, China.

causes disease symptoms in its host plants; however, due to its rapid variability, is difficult to control. To analyze the reason for this pathogenic differentiation, 22 strains with different virulence were isolated from a cotton farm. The genetic diversity of cotton varieties make cotton cultivars have different resistance, so the Xinluzao 7 (susceptible to ), Zhongmian 35 (tolerant), and Xinluzao 33 (resistant) were used to investigate the pathogenicity of the strains in a green house. Vegetative compatibility groups (VCGs) assays, Internal Transcribed Spacer (ITS) PCR, and pathogenicity analysis showed that SHZ-4, SHZ-5, and SHZ-9 had close kinship and significantly different pathogenicity. Transcriptome sequencing of the three strains identified 19 of 146 unigenes in SHZ-4_vs_ SHZ-5, SHZ-5_vs_ SHZ-9, and SHZ-4_vs_ SHZ-9. In these unigenes, three proteinase and four polysaccharide degrading hydrolases were found to be associated with the pathogenicity. However, due to a number of differentially expressed genes in the transport, these unigenes not only played a role in nutrition absorption but might also contribute to the resistance of sugar-induced hyperosmosis. Moreover, the tolerance ability was positively related to the pathogenicity of . This resistance to sugar-induced hyperosmosis might help to access the nutrition of the host. The pathogenicity of correlated with the resistance of sugar-induced-hyperosmosis, which provides clues for the cultivation of resistant varieties.
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http://dx.doi.org/10.7717/peerj.8035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6855202PMC
November 2019