Publications by authors named "Wenhui Liu"

222 Publications

Freeze-thaw condition limits the fermentation process and accelerates the aerobic deterioration of oat () silage in the Qinghai-Tibet Plateau.

Front Microbiol 2022 19;13:944945. Epub 2022 Jul 19.

Sichuan Zoige Alpine Wetland Ecosystem National Observation and Research Station, Southwest Minzu University, Chengdu, China.

The objective of this study is to determine the effect of freeze-thaw condition on the fermentation characteristics, microbial community, and aerobic stability of oat () silage in the Qinghai-Tibet Plateau. Oat forage was harvested at milk ripening stage, ensiled in vacuum-sealed bags, and then stored at (1) a constant temperature of 20°C, as a control (20 group) or (2) subjected to freeze-thaw condition (alternating 20 and -5°C every 12 h; S group). The quality and microbial community in the silage were measured after 1, 3, 7, 14, and 60 days of ensiling, and the aerobic stability was measured after 60 days of ensiling at room temperature or at the two treatment temperatures. The results showed that the higher the pH, the lower the concentration of lactic acid and the ratio of lactic acid/acetic acid of the samples under freeze-thaw condition, as compared to those stored at 20°C. The dry matter content of 20 groups was significantly higher than S group ( < 0.05). While ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), and water-soluble carbohydrates (WSC) had no significant difference between two groups. spp., spp., and spp. were the most prevalent bacterial genera in all groups. The abundance of spp. in the 20 group was the highest on day 3 of ensiling ( < 0.05), and it reached the peak on day 14 in the S group, but the abundance in the S group did not exceed 50% during whole fermentation process. The abundance of Enterobacterales and the count of in the S group was significantly higher than 20 group ( < 0.05). Interestingly, the lactic acid concentration was significant correlated with spp. in 20 group, while correlated with spp. in S group. The aerobic stability of the S group was lower than that of the 20 group ( < 0.05). The present study indicates that the freeze-thaw condition led to insufficient fermentation degree of silage by limiting the fermentation of spp. and severely reduced the aerobic stability of oat silage.
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http://dx.doi.org/10.3389/fmicb.2022.944945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355534PMC
July 2022

Long-term watermelon continuous cropping leads to drastic shifts in soil bacterial and fungal community composition across gravel mulch fields.

BMC Microbiol 2022 08 2;22(1):189. Epub 2022 Aug 2.

School of Agriculture, Ningxia University, 750021, Yinchuan, China.

Despite the known influence of continuous cropping on soil microorganisms, little is known about the associated difference in the effects of continuous cropping on the community compositions of soil bacteria and fungi. Here, we assessed soil physicochemical property, as well as bacterial and fungal compositions across different years (Uncropped control, 1, 6, 11, 16, and 21 years) and in the watermelon system of a gravel mulch field in the Loess Plateau of China. Our results showed that long-term continuous cropping led to substantial shifts in soil bacterial and fungal compositions. The relative abundances of dominant bacterial and fungal genera (average relative abundance > 1.0%) significantly varied among different continuous cropping years (P < 0.05). Structural equation models demonstrated that continuous cropping alter soil bacterial and fungal compositions mainly by causing substantial variations in soil attributes. Variations in soil pH, nutrient, salinity, and moisture content jointly explained 73% and 64% of the variation in soil bacterial and fungal compositions, respectively. Variations in soil moisture content and pH caused by continuous cropping drove the shifts in soil bacterial and fungal compositions, respectively (Mantel R = 0.74 and 0.54, P < 0.01). Furthermore, the variation in soil bacterial and fungal composition showed significant correlation with watermelon yield reduction (P < 0.01). Together, long-term continuous cropping can alter soil microbial composition, and thereby influencing watermelon yield. Our findings are useful for alleviating continuous cropping obstacles and guiding agricultural production.
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http://dx.doi.org/10.1186/s12866-022-02601-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344729PMC
August 2022

Transient Motion Classification Through Turbid Volumes Parallelized Single-Photon Detection and Deep Contrastive Embedding.

Front Neurosci 2022 8;16:908770. Epub 2022 Jul 8.

Department of Biomedical Engineering, Duke University, Durham, NC, United States.

Fast noninvasive probing of spatially varying decorrelating events, such as cerebral blood flow beneath the human skull, is an essential task in various scientific and clinical settings. One of the primary optical techniques used is diffuse correlation spectroscopy (DCS), whose classical implementation uses a single or few single-photon detectors, resulting in poor spatial localization accuracy and relatively low temporal resolution. Here, we propose a technique termed , a new form of DCS that can probe and classify different decorrelating movements hidden underneath turbid volume with high sensitivity using parallelized speckle detection from a 32 × 32 pixel SPAD array. We evaluate our setup by classifying different spatiotemporal-decorrelating patterns hidden beneath a 5 mm tissue-like phantom made with rapidly decorrelating dynamic scattering media. Twelve multi-mode fibers are used to collect scattered light from different positions on the surface of the tissue phantom. To validate our setup, we generate perturbed decorrelation patterns by both a digital micromirror device (DMD) modulated at multi-kilo-hertz rates, as well as a vessel phantom containing flowing fluid. Along with a deep contrastive learning algorithm that outperforms classic unsupervised learning methods, we demonstrate our approach can accurately detect and classify different transient decorrelation events (happening in 0.1-0.4 s) underneath turbid scattering media, without any data labeling. This has the potential to be applied to non-invasively monitor deep tissue motion patterns, for example identifying normal or abnormal cerebral blood flow events, at multi-Hertz rates within a compact and static detection probe.
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http://dx.doi.org/10.3389/fnins.2022.908770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304989PMC
July 2022

Case Report: Combined Cataract Surgery and Minimally Invasive Glaucoma Surgery Provide an Alternative Treatment Approach for Lowe Syndrome.

Front Med (Lausanne) 2022 1;9:913229. Epub 2022 Jul 1.

Department of Ophthalmology, Qilu Hospital, Shandong University, Jinan, China.

We describe the case of a 4-month-old boy who presented with bilateral congenital cataract and high intraocular pressure (IOP) in the left eye, followed by mental retardation and delayed motor development. Genetic investigation revealed the boy had a splicing variant (c.940-11G>A) of the oculocerebrorenal syndrome of Lowe (OCRL) gene. The boy underwent a lensectomy for congenital cataract in his right eye, and lensectomy combined with a 360° suture trabeculotomy to remove the clouded lens and to control IOP of the left eye. During postoperative one-and-a-half-year follow-up, the boy exhibited an improved visual acuity and a well-controlled IOP without the use of topical IOP-lowering medications. Lowe syndrome is a rare multisystemic disorder that is diagnosed through clinical manifestation and genetic testing. The possibility of Lowe syndrome should be considered in patients presenting with typical triad, and genetic analysis should be performed in time to confirm the diagnosis. We recommend combined cataract surgery and minimally invasive glaucoma surgery (MIGS) as a safe, feasible, and efficient method to treat congenital cataract and glaucoma in Lowe syndrome patients.
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http://dx.doi.org/10.3389/fmed.2022.913229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283680PMC
July 2022

Analysis of bacterial diversity and genetic evolution of Lacticaseibacillus paracasei isolates in fermentation pit mud.

J Appl Microbiol 2022 Jul 8. Epub 2022 Jul 8.

Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang, People's Republic of China.

Aims: Since little is known about the genetic diversity of lactic acid bacteria (LAB) isolates from the fermentation pit mud (FPM), we sought to evaluate the bacterial structure, identify the LAB isolates and investigate the genotype and genetic diversity of the LAB isolates.

Methods And Results: Using high-throughput MiSeq sequencing, we identified seven dominant bacterial genera in FPM. Lactobacillus had the highest abundance. We isolated 55 LAB strains. These isolates were all identified as Lacticaseibacillus paracasei. Using an extant multilocus sequence typing (MLST) scheme, isolates were assigned to 18 sequence types (STs) and three clonal complexes. ST1, the largest group, mainly comprised FPM isolates. Niche-specific ST2 to ST18 only contained FPM isolates. Isolates could be divided into four lineages, with most assigned to Lineage 1. Only one FPM isolate was classified as L. paracasei subsp. paracasei. Other isolates could not be classified at the subspecies level using the seven MLST loci.

Conclusions: Lactobacilli account for a high proportion of bacteria in pit mud. Based on the traditional culture method, L. paracasei was the dominant species, and these isolates exhibit a high ethanol tolerance, high intraspecific diversity and specific genetic profiles.

Significance And Impact Of The Study: The study described the characterization of FPM bacterial diversity, giving an insight into the genetic diversity of L. paracasei strains present in FPM.
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http://dx.doi.org/10.1111/jam.15672DOI Listing
July 2022

High-Altitude Genetic Selection and Genome-Wide Association Analysis of Yield-Related Traits in L. Using SLAF Sequencing.

Front Plant Sci 2022 21;13:874409. Epub 2022 Jun 21.

The State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.

The genetic adaptations to harsh climatic conditions in high altitudes and genetic basis of important agronomic traits are poorly understood in L. In this study, an association population of 210 genotypes was used for population structure, selective sweep analysis, and genome-wide association study (GWAS) based on 88,506 single nucleotide polymorphisms (SNPs). We found 965 alleles under the natural selection of high altitude, which included 7 hub genes involved in the response to UV, and flavonoid and anthocyanin biosynthetic process based on the protein-protein interaction (PPI) analysis. Using a mixed linear model (MLM), the GWAS test identified a total of 1,825 significant loci associated with 12 agronomic traits. Based on the gene expression data of two wheat cultivars and the PPI analysis, we finally identified 12 hub genes. Especially, in plant height traits, the top hub gene (TOPLESS protein) encoding auxins and jasmonic acid signaling pathway, shoot apical meristem specification, and xylem and phloem pattern formation was highly overexpressed. These genes might play essential roles in controlling the growth and development of . Therefore, this study provides fundamental insights relevant to hub genes and will benefit molecular breeding and improvement in and other species.
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http://dx.doi.org/10.3389/fpls.2022.874409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253694PMC
June 2022

Detection of Secondary Corneal Lactoferrin Amyloidosis Based on Histologic Biopsies Combined with Mass Spectrometry: A Case Report.

Tohoku J Exp Med 2022 Aug 7;258(1):43-48. Epub 2022 Jul 7.

Department of Radiology, Nanjing Medical University Affiliated Wuxi Second Hospital.

Amyloidosis is characterized by systemic or local deposition of amyloid fibrils outside organs and tissues. Amyloidosis is rarely seen on cornea. A 30-year-old woman patient had had trichiasis in both eyes for 8 years. Trichiasis was observed, which touched the cornea. Slit lamp microscopy showed white gelatinous droplet-like eminences and trichiasis in the lower cornea of the right eye. Optical coherence tomography showed that the lesion involved most of the cornea. Hematoxylin and eosin staining showed that most of the stroma stained red, with scattered inflammatory cells. High expression of lactoferrin was detected by mass spectrometry, and the case was diagnosed as secondary corneal lactoferrin amyloidosis in the right eye.
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http://dx.doi.org/10.1620/tjem.2022.J054DOI Listing
August 2022

Urethane Improves the Response of Auditory Neurons to Tone.

Front Cell Neurosci 2022 15;16:855968. Epub 2022 Jun 15.

The Seventh Affiliated Hospital, Southern Medical University, Foshan, China.

Urethane has little effect on nervous system and is often used in neuroscience studies. However, the effect of urethane in neurons is not thoroughly clear. In this study, we investigated changes in neuron responses to tones in inferior colliculus during urethane anesthesia. As urethane was metabolized, the best and characteristic frequencies did not obviously change, but the minimal threshold (MT) remained relatively stable or was elevated. The frequency tuning bandwidth at 60 dB SPL (BW) remained unchanged or decreased, and the average evoked spike of effective frequencies at 60 dB SPL (ES) gradually decreased. Although the average evoked spike of effective frequencies at a tone intensity of 20 dB SPL above MT (ES) decreased, the frequency tuning bandwidth at a tone intensity of 20 dB SPL above MT (BW) did not change. In addition, the changes in MT, ES, BW, and ES increased with the MT in pre-anesthesia awake state (MT). In some neurons, the MT was lower, BW was broader, and ES and ES were higher in urethane anesthesia state than in pre-anesthesia awake state. During anesthesia, the inhibitory effect of urethane reduced the ES, but did not change the MT, characteristic frequency, or BW. In the recording session with the strongest neuron response, the first spike latency did not decrease, and the spontaneous spike did not increase. Therefore, we conclude that urethane can reduce/not change the MT, increase the evoked spike, or broaden/not change the frequency tuning range, and eventually improve the response of auditory neurons to tone with or without "pushing down" the tonal receptive field in thresholding model. The improved effect increases with the MT of neurons. The changes induced by the inhibitory and improved effects of urethane abide by similar regularities, but the change directions are contrary. The improvement mechanism may be likely due to the increase in the ratio of excitatory/inhibitory postsynaptic inputs to neurons.
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http://dx.doi.org/10.3389/fncel.2022.855968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240349PMC
June 2022

Anti-inflammatory withanolides from the aerial parts of Physalis minima.

Phytochemistry 2022 Jun 30;202:113301. Epub 2022 Jun 30.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300350, People's Republic of China. Electronic address:

Eight undescribed and two known withanolides were obtained from the aerial parts of Physalis minima. The structures of these compounds were defined by spectroscopic methods including 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD) data analysis. Physminin E was elucidated to be a rare 13,14-seco-withanolide. Inhibitory effects of these compounds on nitric oxide (NO) production were evaluated by using LPS-activated RAW264.7 macrophages, and physminin C was shown to be the most active with an IC value of 3.5 μM. The further mechanistic analysis of NO inhibition was performed by molecular docking and Western blotting.
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http://dx.doi.org/10.1016/j.phytochem.2022.113301DOI Listing
June 2022

Adverse childhood experiences in offspring living with parental mental illness: a controlled study from China.

J Ment Health 2022 Jul 1:1-10. Epub 2022 Jul 1.

Peking University Sixth Hospital, Peking University Institute of Mental Health, National Clinical Research Center for Mental Disorders, Beijing, PR China.

Background: Adverse childhood experiences (ACEs) affect children's development, and their harm to health is pervasive throughout the life course.

Aims: To identify ACEs and their risk factors in Chinese household with or without parental mental illness.

Methods: A controlled study was conducted among 181 young adults with parental mental illness (positive group) and 201 demographically matched individuals without parental mental illness (negative group). Univariate and multivariate analyses were performed to study the correlation between ACEs and their risk factors.

Results: The positive group suffered emotional abuse, domestic violence, bullying, and cumulative ACEs more frequently than the negative group. In the positive group, living in rural areas and having a low household economic status during childhood were identified as risk factors for cumulative ACEs, whereas a higher education level of the mother was a protective factor for cumulative ACEs in univariate analyses. Low household economic status remained an independent risk factor for cumulative ACEs in the positive group in multivariate analyses.

Conclusions: Children living with parental mental illness are more vulnerable to ACEs, and our findings highlight the importance of socioeconomic factors in increasing the risk of ACEs. To alleviate the deleterious impact of parental mental illness on offspring, multidimensional supports are needed.
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http://dx.doi.org/10.1080/09638237.2022.2091765DOI Listing
July 2022

Imaging Dynamics Beneath Turbid Media via Parallelized Single-Photon Detection.

Adv Sci (Weinh) 2022 Jun 24:e2201885. Epub 2022 Jun 24.

Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.

Noninvasive optical imaging through dynamic scattering media has numerous important biomedical applications but still remains a challenging task. While standard diffuse imaging methods measure optical absorption or fluorescent emission, it is also well-established that the temporal correlation of scattered coherent light diffuses through tissue much like optical intensity. Few works to date, however, have aimed to experimentally measure and process such temporal correlation data to demonstrate deep-tissue video reconstruction of decorrelation dynamics. In this work, a single-photon avalanche diode array camera is utilized to simultaneously monitor the temporal dynamics of speckle fluctuations at the single-photon level from 12 different phantom tissue surface locations delivered via a customized fiber bundle array. Then a deep neural network is applied to convert the acquired single-photon measurements into video of scattering dynamics beneath rapidly decorrelating tissue phantoms. The ability to reconstruct images of transient (0.1-0.4 s) dynamic events occurring up to 8 mm beneath a decorrelating tissue phantom with millimeter-scale resolution is demonstrated, and it is highlighted how the model can flexibly extend to monitor flow speed within buried phantom vessels.
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http://dx.doi.org/10.1002/advs.202201885DOI Listing
June 2022

Viral keratitis after the second and third doses of inactivated COVID-19 vaccination: A case report.

Hum Vaccin Immunother 2022 Jun 22:2090177. Epub 2022 Jun 22.

Department of ophthalmology, Qilu Hospital of Shandong University, Jinan, Shandong, China.

Rare cases of viral keratitis after coronavirus disease 2019 (COVID-19) vaccination have been reported. Furthermore, to our knowledge, cases of viral keratitis after two rounds of COVID-19 vaccination have not yet been reported. We report the case of a 19-year-old man without a history of keratitis, who developed viral keratitis soon after receiving the second and third doses of inactivated COVID-19 vaccines. Each time after the patient received treatment with topical and systemic drugs, his ocular symptoms were gradually relieved, and corrected visual acuity in both eyes returned to normal. COVID-19 vaccination may be associated with rare cases of the development of keratitis in individuals without a medical history of keratitis. Physicians should be aware of the possible relationship between ocular symptoms and adverse reactions to the COVID-19 vaccination. Despite the potential risks of COVID-19 vaccination, the benefits of immunization against the virus far outweigh these risks.
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http://dx.doi.org/10.1080/21645515.2022.2090177DOI Listing
June 2022

Patient Symptoms and Stress Testing After Elective Percutaneous Coronary Intervention in the Veterans Affairs Health Care System.

JAMA Netw Open 2022 06 1;5(6):e2217704. Epub 2022 Jun 1.

Division of Cardiology, University of Colorado Anschutz Medical Campus, Aurora.

Importance: Up to 60% of patients in the US receive a stress test within 2 years of percutaneous coronary intervention (PCI), prompting concerns about the possible overuse of stress testing.

Objective: To examine the proportion of patients who underwent stress testing within 2 years of elective PCI, proportion of patients who had symptoms that were consistent with coronary artery disease (CAD), timing of stress testing, and site-level variation in stress testing among symptomatic and asymptomatic patients.

Design, Setting, And Participants: This cohort study used administrative claims data and clinical records from the US Department of Veterans Affairs (VA) Clinical Assessment, Reporting, and Tracking program. Patients who underwent stress testing within 2 years of elective PCI for stable CAD between November 1, 2013, and October 31, 2015, at 64 VA facilities were included in the analysis. Patients who received stress testing for staging purposes, cardiac rehabilitation evaluation, or preoperative testing before high-risk surgery were excluded. Data were analyzed from June to December 2020.

Main Outcomes And Measures: The main outcome was the proportion of patients who underwent stress testing and had symptoms that were consistent with obstructive CAD, using definitions from the 2013 clinical practice guideline (Multimodality Appropriate Use Criteria for the Detection and Risk Assessment of Stable Ischemic Heart Disease). Secondary outcomes were the timing of stress testing (assessed using a cumulative incidence curve) and site-level variation in stress testing (assessed using multilevel logistic regression models).

Results: A total of 3705 consecutive patients (mean [SD] age 66.3 [7.6] years; 3656 men [98.7%]; 437 Black individuals [11.8%], 3175 White individuals [85.7%], and 93 individuals [2.5%] of other races and ethnicities [Asian, Hispanic or Latinx, or unknown]) had elective PCI. Of these patients, 916 (24.7%) received a stress test within 2 years, among whom 730 (79.7%) had symptoms that were consistent with obstructive CAD at the time of stress testing. Visual inspection of a cumulative incidence curve for stress testing showed no rapid increases in stress testing at 6 months or 1 year after PCI, which might coincide with routine clinical visits. The proportion of symptomatic patients who underwent stress testing at each VA site ranged from 67.7% to 100%, with no significant site-level variation in stress testing.

Conclusions And Relevance: Results of this study suggest that most veterans who underwent stress testing within 2 years after elective PCI had symptoms that were consistent with obstructive CAD. Therefore, measuring low-value stress testing using only administrative claims data may overestimate its prevalence, and concerns about overuse of post-PCI stress testing may be overstated.
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http://dx.doi.org/10.1001/jamanetworkopen.2022.17704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214585PMC
June 2022

Effects of the COVID-19 Lockdown on Air Pollutant Levels and Associated Reductions in Ischemic Stroke Incidence in Shandong Province, China.

Front Public Health 2022 27;10:876615. Epub 2022 May 27.

Department of Epidemiology, School of Public Health, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background: Local governments in China took restrictive measures after the outbreak of COVID-19 to control its spread, which unintentionally resulted in reduced anthropogenic emission sources of air pollutants. In this study, we intended to examine the effects of the COVID-19 lockdown policy on the concentration levels of particulate matter with aerodynamic diameters of ≤1 μm (PM), ≤2.5 μm (PM), and ≤10 μm (PM), nitrogen dioxide (NO), sulfur dioxide (SO), ozone (O), and carbon monoxide (CO) and the potential subsequent reductions in the incidence of ischemic and hemorrhagic stroke in Shandong Province, China.

Methods: A difference-in-difference model combining the daily incidence data for ischemic and hemorrhagic stroke and air pollutant data in 126 counties was used to estimate the effect of the COVID-19 lockdown on the air pollutant levels and ischemic and hemorrhagic stroke incident counts. The avoided ischemic stroke cases related to the changes in air pollutant exposure levels were further estimated using concentration-response functions from previous studies.

Results: The PM, PM, PM, NO, and CO levels significantly decreased by -30.2, -20.9, -13.5, -46.3, and -13.1%, respectively. The O level increased by 11.5% during the lockdown compared with that in the counterfactual lockdown phase of the past 2 years. There was a significant reduction in population-weighted ischemic stroke cases (-15,315, 95% confidence interval []: -27,689, -2,942), representing a reduction of 27.6% (95% : -49.9%, -5.3%). The change in the number of hemorrhagic stroke cases was not statistically significant. The total avoided PM-, PM-, PM-, NO-, and CO-related ischemic stroke cases were 739 (95% : 641, 833), 509 (95% : 440, 575), 355 (95% : 304, 405), 1,132 (95% : 1,024, 1,240), and 289 (95% : 236, 340), respectively.

Conclusion: The COVID-19 lockdown indirectly reduced the concentration levels of PM, PM, PM, NO, and CO and subsequently reduced the associated ischemic stroke incidence. The health benefits due to the lockdown are temporary, and long-term measures should be implemented to increase air quality and related health benefits in the post-COVID-19 period.
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http://dx.doi.org/10.3389/fpubh.2022.876615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197688PMC
May 2022

Gut Microbiota and Antidiabetic Drugs: Perspectives of Personalized Treatment in Type 2 Diabetes Mellitus.

Front Cell Infect Microbiol 2022 31;12:853771. Epub 2022 May 31.

Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China.

Alterations in the composition and function of the gut microbiota have been reported in patients with type 2 diabetes mellitus (T2DM). Emerging studies show that prescribed antidiabetic drugs distort the gut microbiota signature associated with T2DM. Even more importantly, accumulated evidence provides support for the notion that gut microbiota, in turn, mediates the efficacy and safety of antidiabetic drugs. In this review, we highlight the current state-of-the-art knowledge on the crosstalk and interactions between gut microbiota and antidiabetic drugs, including metformin, α-glucosidase inhibitors, glucagon-like peptide-1 receptor agonists, dipeptidyl peptidase-4 inhibitors, sodium-glucose cotransporter 2 inhibitors, traditional Chinese medicines and other antidiabetic drugs, as well as address corresponding microbial-based therapeutics, aiming to provide novel preventative strategies and personalized therapeutic targets in T2DM.
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http://dx.doi.org/10.3389/fcimb.2022.853771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194476PMC
May 2022

Identification of Flowering Regulatory Networks and Hub Genes Expressed in the Leaves of L. Using Comparative Transcriptome Analysis.

Front Plant Sci 2022 16;13:877908. Epub 2022 May 16.

The State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.

Flowering is a significant stage from vegetative growth to reproductive growth in higher plants, which impacts the biomass and seed yield. To reveal the flowering time variations and identify the flowering regulatory networks and hub genes in , we measured the booting, heading, and flowering times of 66 accessions. The booting, heading, and flowering times varied from 136 to 188, 142 to 194, and 148 to 201 days, respectively. The difference in flowering time between the earliest- and the last-flowering accessions was 53 days. Furthermore, transcriptome analyses were performed at the three developmental stages of six accessions with contrasting flowering times. A total of 3,526 differentially expressed genes (DEGs) were predicted and 72 candidate genes were identified, including transcription factors, known flowering genes, and plant hormone-related genes. Among them, four candidate genes (, and were significantly upregulated in late-flowering accessions. , and were identified as hub genes in the turquoise and blue modules which were related to the development time of flowering by weighted gene co-expression network analysis (WGCNA). A single-nucleotide polymorphism (SNP) of found by multiple sequence alignment may cause late flowering. The expression pattern of flowering candidate genes was verified in eight flowering promoters (, and ) and four flowering suppressors (, and ) under drought and salt stress by qRT-PCR. The results suggested that drought and salt stress activated the flowering regulation pathways to some extent. The findings of the present study lay a foundation for the functional verification of flowering genes and breeding of new varieties of early- and late-flowering .
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http://dx.doi.org/10.3389/fpls.2022.877908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150504PMC
May 2022

Glucagon-receptor-antagonism-mediated β-cell regeneration as an effective anti-diabetic therapy.

Cell Rep 2022 May;39(9):110872

NGM Biopharmaceuticals, South San Francisco, CA 94080, USA.

Type 1 diabetes mellitus (T1D) is a chronic disease with potentially severe complications, and β-cell deficiency underlies this disease. Despite active research, no therapy to date has been able to induce β-cell regeneration in humans. Here, we discover the β-cell regenerative effects of glucagon receptor antibody (anti-GcgR). Treatment with anti-GcgR in mouse models of β-cell deficiency leads to reversal of hyperglycemia, increase in plasma insulin levels, and restoration of β-cell mass. We demonstrate that both β-cell proliferation and α- to β-cell transdifferentiation contribute to anti-GcgR-induced β-cell regeneration. Interestingly, anti-GcgR-induced α-cell hyperplasia can be uncoupled from β-cell regeneration after antibody clearance from the body. Importantly, we are able to show that anti-GcgR-induced β-cell regeneration is also observed in non-human primates. Furthermore, anti-GcgR and anti-CD3 combination therapy reverses diabetes and increases β-cell mass in a mouse model of autoimmune diabetes.
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http://dx.doi.org/10.1016/j.celrep.2022.110872DOI Listing
May 2022

Therapeutic Effects of Synthetic Triblock Amphiphilic Short Antimicrobial Peptides on Human Lung Adenocarcinoma.

Pharmaceutics 2022 Apr 24;14(5). Epub 2022 Apr 24.

Department of Pathology and Pathophysiology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China.

Because of their unique properties, antimicrobial peptides (AMPs) represent a potential reservoir of novel anticancer therapeutic agents. However, only a few AMPs can kill tumors with high efficiency, and obtaining inexpensive anticancer AMPs with strong activity is still a challenge. In our previous work, a series of original short amphiphilic triblock AMP (KFK) analogues were developed which were demonstrated to exert excellent effects on bacterial infection, both in vitro and in vivo. Herein, the overall objectives were to assess the potent tumoricidal capacities of these analogues against human lung cancer cell line A549 and the underlying mechanism. The results of the CCK-8 assay revealed that the precise modification of the peptides' primary sequences could modulate their tumoricidal potency. In the tumoricidal progress, positive charge and hydrophobicity were the key driving forces. Among these peptides, KFK displayed the most remarkable tumoricidal activity. Furthermore, the excellent anticancer capacity of KFK was proven by the live/dead cell staining, colony formation assay, and tumor growth observations on xenografted mice, which indicated that KFK might be a promising drug candidate for lung cancer, with no significant adverse effects in vitro or in vivo. In addition, the cell apoptosis assay using flow cytometry, the morphology observations using the optical microscope, confocal microscopy using CellMask™ Deep Red staining, and scanning electron microscope suggested that membrane disruption was the primary mechanism of its antitumor action. Through analyzing the structure-activity relationship, it was found that the amount of positive charge required for KFK to exert its optimal tumoricidal effect was more than that needed for the antimicrobial activity, while the optimal proportion of hydrophobicity was less. Our findings suggest that further analysis of the structure-activity relationship of AMPs' primary sequence variations will be beneficial. Hopefully, this work can provide guiding principles in designing peptide-based therapeutics for lung cancer.
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http://dx.doi.org/10.3390/pharmaceutics14050929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143638PMC
April 2022

Wavelength Modulation Characteristics of Metal Gratings on Si-Based Blocked-Impurity-Band (BIB) Terahertz Detectors.

Micromachines (Basel) 2022 May 23;13(5). Epub 2022 May 23.

Shanghai Microwave Technology Research Institute, Shanghai 200063, China.

In this work, the wavelength selection characteristics of metal gratings on Si-based blocked-impurity-band (BIB) detectors in the terahertz band were studied by performing experiments and a finite difference time domain (FDTD) simulation. The transmission spectra of metal gratings with different periods on 130 μm intrinsic Si substrates were measured. When the metal grating period increased from 16 to 20 to 32 μm, the peak position of the spectrum moved from 21.71 to 24.50 to 36.59 μm, which is in good agreement with the FDTD simulation results. The structure with the period of 32 μm shows the best wavelength selective transmission characteristics. Then, the bare Si-based BIB devices and metal grating/Si-based BIB hybrid devices with different thicknesses of blocking layers of 2 and 5 μm were fabricated. By covering different periods of metal gratings for the devices with a thicker blocking layer of 2 μm, we obtained more effective wavelength selection characteristics and stronger response spectra enhancement ratios that were about 1.3, 2.4, or 1.9 times. This was mainly due to the localized optical field enhancement effect of the plasmons resonance in metal gratings, which decays exponentially in a vertical direction. Our results demonstrate a new approach for the Si-based BIB detector to realize multiband selective detection applications.
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http://dx.doi.org/10.3390/mi13050811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144587PMC
May 2022

Complete Chloroplast Genome Sequence of , Insights into Comparative Chloroplast Genomics, Divergence Time Estimation and Phylogenetic Relationships among Dipsacales.

Genes (Basel) 2022 05 23;13(5). Epub 2022 May 23.

Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.

, ( L., Caprifoliaceae, Dipsacales) are widely distributed in China while mainly occurrs in northeast China. Few reports have been determined on the genus . In the present research, we sequenced 2 chloroplast genomes of and analyzed 18 chloroplast genomes, trying to explore the sequence variations and phylogeny of genus in the order Dipsacales. The chloroplast genomes of the genus ranged from 154,579 bp to 157,178 bp, consisting of 132 genes (86 protein-coding genes, 38 transfer RNA genes, and 8 ribosomal RNA genes). Comparative analyses and phylogenetic analysis supported the division of Dipsacales into two clades, Adoxaceae and six other families. Among the six families, a clade of Valerianaceae+Dipsacaceae was recovered as a sister to a clade of Morinaceae+Linnaeaceae. A closer relationship of and among three species was revealed. Our research supported that and was closely related. had a closer relationship with and than Morinaceae. The divergence between and two other species in was dated to 13.4 mya.
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http://dx.doi.org/10.3390/genes13050933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141360PMC
May 2022

Association between short-term exposure to ambient PM and PM and forced vital capacity in Chinese children and adolescents.

Environ Sci Pollut Res Int 2022 May 23. Epub 2022 May 23.

Department of Epidemiology, School of Public Health, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

This study aims to examine the association between short-term exposure to ambient PM, PM, and PM and forced vital capacity (FVC). Population data were obtained from a school-based cross-sectional survey in Shandong in 2014. Distributed lag non-linear models were used to examine the association between exposure to PM, PM, and PM and FVC at the day of FVC measurement and the previous 6 days (lag 0 to 6 days). A total of 35,334 students aged 9 to 18 years were included in the study, and the mean exposure concentrations of ambient PM, PM, and PM for them were 47.4 (standard deviation [SD] = 21.3) μg/m, 32.8 (SD = 32.2) μg/m, and 80.1 (SD = 47.7) μg/m, respectively. An inter-quartile range (IQR, 24 μg/m) increment in exposure to PM was significantly associated with a lower FVC at lag 0 and lag 1 day (β =  - 80 mL, 95% CI =  - 119, - 42, and β =  - 37 mL, 95% CI =  - 59, - 16, respectively), and an IQR (54 μg/m) increment in exposure to PM was significantly associated with a lower FVC at lag 0 and lag 1 day (β =  - 57 mL, 95% CI =  - 89, - 18, and β =  - 34 mL, 95% CI =  - 56, - 12, respectively) after adjustment for gender, age, body mass index category, residence, month of the survey, intake of eggs, intake of milk, physical activity, and screen time. No significant associations were observed for PM. The inverse associations of PM and PM with FVC were larger in males, younger children, those overweight or obese, and those with insufficient physical activity levels. Short-term exposure to ambient PM and PM was associated with decreased FVC, and PM may be the primary fraction of PM causing the adverse pulmonary effects. Our findings emphasize the need to address ambient PM, especially PM, pollution for affecting pulmonary health in children and adolescents.
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http://dx.doi.org/10.1007/s11356-022-20842-6DOI Listing
May 2022

Physicochemical and structural properties of dietary fiber from pomace by steam explosion.

J Food Sci Technol 2022 Jun 16;59(6):2381-2391. Epub 2021 Sep 16.

Beijing Key Laboratory of Forest Food Process and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, No.35, Qinghua East Road, Beijing, 100083 People's Republic of China.

pomace was treated by steam explosion (SE) at 0.87 MPa for 97 s. After SE treatment, the Insoluble dietary fiber (IDF) content of pomace decreased from 45.13 ± 0.23 to 30.01 ± 0.15%, and the soluble dietary fiber (SDF) content increased from 9.31 ± 0.07 to 15.82 ± 0.31%. The structure of IDF and SDF after SE showed that the original compact structures were destroyed, and the specific surface areas increased. Thermal analysis showed that the thermal stability of the modified SDF was improved. However, SE did not change the crystal structure and functional group composition of IDF and SDF. Physicochemical analysis indicated that IDF had better hydration capacity after SE treatment, and the oil-holding capacities of IDF and SDF were also significantly improved. SE is an effective method to improve the utilization of pomace and a feasible method for modification of dietary fiber.
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http://dx.doi.org/10.1007/s13197-021-05254-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114244PMC
June 2022

The activated synaptic terminals beyond the light illumination range affect the results of optogenetics.

Neuroreport 2022 05 8;33(7):281-290. Epub 2022 Apr 8.

Department of Physiology, School of Basic Medical Sciences, Key Laboratory of Psychiatric Disorders of Guangdong Province, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Key Laboratory of Mental Health of the Ministry of Education, Southern Medical University, Guangzhou and.

Objectives: Optogenetics is widely applied to study complex brain networks. However, recent studies have found that light alone can produce effects that are unrelated to optogenetics, and it is still unclear whether this can affect the results of optogenetic experiments.

Methods: We explored the characteristics of projection of interneurons to excitatory neurons in the auditory cortex with optogenetics, transgenic mice and patch-clamp recording.

Results: We discovered that postsynaptic responses can be induced when we stimulated a blank area adjacent to the edge of brain slice. Similar results can be observed after blocking the polysynaptic responses by drugs. Together with the results of control experiments, we found that the false response is caused by activating the synaptic terminals beyond the range of the blue light (470 nm). Also, there was a linear relationship between the response and the stimulus distance for all data, which suggested that these false responses may be related to other factors, such as light scattering.

Conclusions: The LED-light-evoked response cannot reflect microcircuit of the recorded neuron and the activated neurons within the illumination range accurately. Together, these results confirm that light alone can affect neural activity, but this can be unrelated to the genuine 'optogenetic effect'.
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http://dx.doi.org/10.1097/WNR.0000000000001785DOI Listing
May 2022

Establishment of a scoring model for early diagnosis of infection associated with liver failure.

Ann Hepatol 2022 Jul-Aug;27(4):100713. Epub 2022 Apr 29.

Department of Infectious Diseases, First Affiliated Hospital, Soochow University, Suzhou, 215006, Jiangsu Province, China. Electronic address:

Introduction And Objectives: Infection is a common complication of liver failure. Serum inflammatory markers used to diagnose infection have sufficient diagnostic sensitivity but low specificity. This study aimed to improve the early diagnosis of infections in liver failure patients by developing a diagnostic model and evaluating its predictive ability.

Patients And Methods: A retrospective analysis of clinical data from liver failure patients. Cases were divided into infected and non-infected groups according to their clinical diagnosis. Nine infection-related predictors (age, body temperature, neutrophil ratio (NE%), procalcitonin (PCT), C-reactive protein (CRP), lactic acid (Lac), serum albumin (Alb), model of end-stage liver disease (MELD) score, and sequential organ failure assessment (SOFA) score) were included in multivariate logistic regression analysis. The diagnostic model was validated, and the receiver operating characteristic (ROC) curve was used to analyze its predictive accuracy.

Results: In the model group, multivariate logistic regression analysis showed that age, body temperature, PCT, CRP, Lac, and SOFA score were independent predictors of infection associated with liver failure (P < 0.05). The area under the ROC curve (AUC) of the model was 0.899 (95% confidence interval [CI] 0.846-0.939), and the sensitivity and specificity were 86.2% and 80.4%, respectively. The AUC for the validation group was 0.953 (95% CI 0.899-0.983), and the sensitivity and specificity were 91.7% and 84.2%, respectively.

Conclusions: This study reports a model for early diagnosis of infection in liver failure patients. The model had high overall accuracy and showed good reproducibility and reliability in patients from different centers in the same region.
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http://dx.doi.org/10.1016/j.aohep.2022.100713DOI Listing
July 2022

Short-term effects of exposure to ambient PM, PM, and PM on ischemic and hemorrhagic stroke incidence in Shandong Province, China.

Environ Res 2022 09 27;212(Pt C):113350. Epub 2022 Apr 27.

Department of Epidemiology, School of Public Health, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. Electronic address:

Background: Short-term exposure to ambient PM and PM is associated with increased risk of mortality and hospital admissions for stroke. However, there is less evidence regarding the effect of exposure to PM on stroke incidence. We estimated the incidence risk of stroke and the attributable fractions related to short-term exposure to ambient PM, PM and PM in China.

Methods: County-specific incidence of stroke was obtained from health statistics in years 2014-2019. We linked county-level mean daily concentrations of PM, PM and PM with stroke incidence. We used the time stratified case-crossover design to estimate the associations between stroke incidence and exposure to PM, PM and PM. We also estimated the disease burden fractions attributable to PM, PM, and PM.

Results: The study included a total of 2,193,954 stroke, from which 1,861,331 were ischemic and 332,623 were hemorrhagic stroke. PM, PM, and PM levels were associated with increased risks of total stroke and ischemic stroke at when assessing the associations in exposure at lag0-4 days. The increase of 10 μg/m in PM, PM, and PM was associated with total stroke, and the relative risks were 1.012 (95% confidence interval: 1.008, 1.015), 1.006 (1.004, 1.007) and 1.003 (1.002, 1.004), while the associations with ischemic stroke were 1.013 (1.010, 1.017), 1.006 (1.005, 1.008) and 1.003 (1.002, 1.004), respectively. There was no significant association between PM and risk of hemorrhagic stroke. The attributable fractions of total stroke were 6.9% (5.1%, 8.5%), 5.6% (4.2%, 6.8%) and 5.6% (3.9%, 7.1%) for PM, PM, and PM, respectively.

Conclusions: PM showed a stronger association with stroke, with a larger attributable fraction of outcomes, than PM and PM. Clean air policies should target the whole scope of PM, including PM.
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http://dx.doi.org/10.1016/j.envres.2022.113350DOI Listing
September 2022

Leukemia inhibitory factor protects against liver steatosis in nonalcoholic fatty liver disease patients and obese mice.

J Biol Chem 2022 06 18;298(6):101946. Epub 2022 Apr 18.

Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, China; Guangdong Provincial Key Laboratory of Shock and Microcirculation, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address:

Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide. However, the molecular mechanisms that promote dysregulation of hepatic triglyceride metabolism and lead to NAFLD are poorly understood, and effective treatments are limited. Leukemia inhibitory factor (LIF) is a member of the interleukin-6 cytokine family and has been shown to regulate a variety of physiological processes, although its role in hepatic triglyceride metabolism remains unknown. In the present study, we measured circulating LIF levels by ELISA in 214 patients with biopsy-diagnosed NAFLD as well as 314 normal control patients. We further investigated the potential role and mechanism of LIF on hepatic lipid metabolism in obese mice. We found that circulating LIF levels correlated with the severity of liver steatosis. Patients with ballooning, fibrosis, lobular inflammation, and abnormally elevated liver injury markers alanine transaminase and aspartate aminotransferase also had higher levels of serum LIF than control patients. Furthermore, animal studies showed that white adipose tissue-derived LIF could ameliorate liver steatosis through activation of hepatic LIF receptor signaling pathways. Together, our results suggested that targeting LIF-LIF receptor signaling might be a promising strategy for treating NAFLD.
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http://dx.doi.org/10.1016/j.jbc.2022.101946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123280PMC
June 2022

Polyethyleneimine Functionalized Mesoporous Magnetic Nanoparticles with Enhanced Antibacterial and Antibiofilm Activity in an Alternating Magnetic Field.

ACS Appl Mater Interfaces 2022 Apr 14;14(16):18794-18805. Epub 2022 Apr 14.

Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang 110819, China.

Despite a lot of research on the antibacterial effect of FeO nanoparticles, their interactions with biofilm matrix have not been well understood. The surface charge of nanoparticles mainly determines their ability to adhere on the biofilm. In this work, negatively charged FeO nanoparticles were synthesized via a trisodium citrate-assisted solvothermal method and then the surfaces were functionalized using polyethyleneimine (PEI) to obtain positively charged FeO nanoparticles. The antibacterial and antibiofilm activities of both negatively and positively charged FeO nanoparticles in an alternating magnetic field were then systematically investigated. The positively charged FeO nanoparticles showed a strong self-adsorbed attachment ability to the planktonic and sessile cells, resulting in a better antibacterial activity and enhanced biofilm eradication performance compared to the conventional FeO nanoparticles with negative charges. [email protected] nanoparticles produced physical stress and thermal damage in response to an alternating magnetic field, inducing the accumulation of intracellular reactive oxygen species into live bacterial cells, bacterial membrane damage, and biofilm dispersion. Utilizing an alternating magnetic field along with positively charged nanoparticles leads to a synergistic antibacterial approach to improve the antibiofilm performance of magnetic nanoparticles.
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http://dx.doi.org/10.1021/acsami.1c24148DOI Listing
April 2022

Silencing of MsD14 Resulted in Enhanced Forage Biomass through Increasing Shoot Branching in Alfalfa ( L.).

Plants (Basel) 2022 Mar 30;11(7). Epub 2022 Mar 30.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Branching is one of the key determinants of plant architecture that dramatically affects crop yield. As alfalfa is the most important forage crop, understanding the genetic basis of branching in this plant can facilitate breeding for a high biomass yield. In this study, we characterized the strigolactone receptor gene in alfalfa and demonstrated that was predominantly expressed in flowers, roots, and seedpods. Furthermore, we found that expression could significantly respond to strigolactone in alfalfa seedlings, and its protein was located in the nucleus, cytoplasm, and cytomembrane. Most importantly, transformation assays demonstrated that silencing of in alfalfa resulted in increased shoot branching and forage biomass. Significantly, MsD14 could physically interact with AtMAX2 and MsMAX2 in the presence of strigolactone, suggesting a similarity between MsD14 and AtD14. Together, our results revealed the conserved D14-MAX2 module in alfalfa branching regulation and provided candidate genes for alfalfa high-yield molecular breeding.
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http://dx.doi.org/10.3390/plants11070939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9003486PMC
March 2022

Trends in Timing of and Glycemia at Initiation of Second-line Type 2 Diabetes Treatment in U.S. Adults.

Diabetes Care 2022 06;45(6):1335-1345

Medicine Service, U.S. Department of Veterans Affairs, Eastern Colorado Health Care System, Aurora, CO.

Objective: Therapeutic inertia threatens the potential long-term benefits of achieving early glycemic control after type 2 diabetes diagnosis. We evaluated temporal trends in second-line diabetes medication initiation among individuals initially treated with metformin.

Research Design And Methods: We included data from 199,042 adults with type 2 diabetes in the U.S. Department of Veterans Affairs health care system initially treated with metformin monotherapy from 2005 to 2013. We used multivariable Cox proportional hazards and linear regression to estimate associations of year of metformin monotherapy initiation with time to second-line diabetes treatment over 5 years of follow-up (primary outcome) and with hemoglobin A1c (HbA1c) at the time of second-line diabetes treatment initiation (secondary outcome).

Results: The cumulative 5-year incidence of second-line medication initiation declined from 47% among metformin initiators in 2005 to 36% in 2013 counterparts (P < 0.0001) despite a gradual increase in mean HbA1c at the end of follow-up (from 6.94 ± 1.28% to 7.09 ± 1.42%, Ptrend < 0.0001). In comparisons with metformin monotherapy initiators in 2005, adjusted hazard ratios for 5-year initiation of second-line diabetes treatment ranged from 0.90 (95% CI 0.87, 0.92) for 2006 metformin initiators to 0.68 (0.66, 0.70) for 2013 counterparts. Among those receiving second-line treatment within 5 years of metformin initiation, HbA1c at second-line medication initiation increased from 7.74 ± 1.66% in 2005 metformin initiators to 8.55 ± 1.92% in 2013 counterparts (Ptrend < 0.0001).

Conclusions: We observed progressive delays in diabetes treatment intensification consistent with therapeutic inertia. Process-of-care interventions early in the diabetes disease course may be needed to reverse adverse temporal trends in diabetes care.
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http://dx.doi.org/10.2337/dc21-2492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210868PMC
June 2022

Upregulation of IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression That Is Related to Cell Proliferation, Metastasis and Tumor-Infiltrating Immune Cells.

Front Oncol 2022 1;12:809589. Epub 2022 Mar 1.

Department of Stomatology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

The strong invasive and metastatic abilities of oral squamous cell carcinoma (OSCC) cells in the early stage are the main reason for its poor prognosis. The early diagnosis and treatment of OSCC may reduce the metastasis rate and improve the survival rate. The aim of this study was to explore candidate biomarkers related to the prognosis and progression of OSCC. We performed weighted gene coexpression network analysis to identify key modules and genes associated with OSCC and intersected the differentially expressed genes (DEGs) in The Cancer Genome Atlas (TCGA)-OSCC and GSE30784 datasets. Next, we performed survival analysis and immunohistochemistry to screen and validate the hub gene insulin-like growth factor 2 (IGF2) mRNA binding protein 2 IGF2BP2. We also used TCGA pan-cancer data to verify that IGF2BP2 was expressed at high levels in a variety of cancers and was related to a poor prognosis in patients. Furthermore, we divided patients with OSCC into high and low expression groups based on the median expression level of IGF2BP2. Gene set enrichment analysis (GSEA) showed that IGF2BP2 led to a poor prognosis in OSCC by affecting cancer-related (epithelial-mesenchymal transition, glycolysis, cell cycle, etc.) and immune-related biological functions and pathways. Single-sample GSEA (ssGSEA), CIBERSORT, and xCell algorithms helped reveal that high IGF2BP2 expression was accompanied by a significant reduction in the immune score, stromal score, and microenvironment score and a decrease in the number of infiltrating CD8+ T cells in OSCC. In addition, silencing IGF2BP2 suppressed the proliferation, migration, and invasion of OSCC cells. In general, IGF2BP2 is a potential biomarker for the progression, immunotherapy response, and prognosis of OSCC.
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http://dx.doi.org/10.3389/fonc.2022.809589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8921988PMC
March 2022
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