Publications by authors named "Wenfeng Chen"

82 Publications

Deciphering the structure, function, expression and regulation of aquaporin-5 in cancer evolution.

Oncol Lett 2021 Apr 21;21(4):309. Epub 2021 Feb 21.

Department of Respiratory, Weifang People's Hospital, Weifang, Shandong 261000, P.R. China.

In recent years, the morbidity rate resulting from numerous types of malignant tumor has increased annually, and the treatment of tumors has been attracting an increasing amount of attention. A number of recent studies have revealed that the water channel protein aquaporin-5 (AQP5) has become a major player in multiple types of cancer. AQP5 is abnormally expressed in a variety of tumor tissues or cells and has multiple effects on certain biological functions of tumors, such as regulating the proliferation, apoptosis and migration of tumor cells. It has been suggested that AQP5 may play an important role in the process of tumor development, opening up a new field of tumor research. The present review highlighted the structure of AQP5 and its role in tumor progression. Furthermore, the expression of AQP5 in different malignant neoplasms was summarized. In addition, the influence of not only drugs, but also different compounds on AQP5 were summarized. In conclusion, according to the findings in the present review, AQP5 has potential as a novel therapeutic target in human cancer, and other AQPs should be similarly investigated.
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http://dx.doi.org/10.3892/ol.2021.12571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905525PMC
April 2021

Comprehensive estimation of lake volume changes on the Tibetan Plateau during 1976-2019 and basin-wide glacier contribution.

Sci Total Environ 2021 Jun 30;772:145463. Epub 2021 Jan 30.

LEGOS, Université de Toulouse CNES, CNRS, IRD, UPS, F-31400 Toulouse, France.

Volume changes and water balances of the lakes on the Tibetan Plateau (TP) are spatially heterogeneous and the lake-basin scale drivers remain unclear. In this study, we comprehensively estimated water volume changes for 1132 lakes larger than 1 km and determined the glacier contribution to lake volume change at basin-wide scale using satellite stereo and multispectral images. Overall, the water mass stored in the lakes increased by 169.7 ± 15.1 Gt (3.9 ± 0.4 Gt yr) between 1976 and 2019, mainly in the Inner-TP (157.6 ± 11.6 or 3.7 ± 0.3 Gt yr). A substantial increase in mass occurred between 1995 and 2019 (214.9 ± 12.7 Gt or 9.0 ± 0.5 Gt yr), following a period of decrease (-45.2 ± 8.2 Gt or -2.4 ± 0.4 Gt yr) prior to 1995. A slowdown in the rate of water mass increase occurred between 2010 and 2015 (23.1 ± 6.5 Gt or 4.6 ± 1.3 Gt yr), followed again by a high value between 2015 and 2019 (65.7 ± 6.7 Gt or 16.4 ± 1.7 Gt yr). The increased lake-water mass occurred predominately in glacier-fed lakes (127.1 ± 14.3 Gt) in contrast to non-glacier-fed lakes (42.6 ± 4.9 Gt), and in endorheic lakes (161.9 ± 14.0 Gt) against exorheic lakes (7.8 ± 5.8 Gt) over 1976-2019. Endorheic and glacier-fed lakes showed strongly contrasting patterns with a remarkable storage increase in the northern TP and slight decrease in the southern TP. The ratio of excess glacier meltwater runoff to lake volume increase between 2000 and ~2019 was less than 30% for the entire Inner-TP based on several independent data sets. Among individual lake-basins, 14 showed a glacier contribution to lake volume increase of 0.3% to 29.1%. The other eight basins exhibited a greater glacier contribution of 116% to 436%, which could be explained by decreased net precipitation. The lake volume change and basin scale glacier contribution reveal that the enhanced precipitation predominantly drives lake volume increase but it is spatially heterogeneous.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145463DOI Listing
June 2021

Pharmaceuticals and personal care products in the seawater around a typical subtropical tourist city of China and associated ecological risk.

Environ Sci Pollut Res Int 2021 Jan 10. Epub 2021 Jan 10.

Ministry of Natural Resources of China, Third Institute of Oceanography, No. 178, Daxue Road, Siming District, Xiamen, 361005, Fujian, China.

Pharmaceuticals and personal care products (PPCPs) in the sea area surrounding a densely populated tourist city in southeastern China were investigated. In total, 32 PPCP pollutants classified into 23 categories were detected. Different spatial distribution patterns of PPCPs indicated possible contamination from runoff and multiple local sources. The labile-to-conservative ratios of PPCPs showed the influence of untreated domestic sewage. In addition, increased concentrations of ciprofloxacin, enrofloxacin, and erythromycin around aquaculture farms imply that aquaculture cannot be neglected as a source. The concentrations of oxytetracycline, ranitidine, ciprofloxacin, miconazole, and sulfamethizole were higher in the wet season than those in the dry season, and the difference in pharmaceutical consumption was suspected to be the main driving factor of this seasonal variation. The risk quotients calculated with the maximum concentrations of miconazole, triclosan, dehydronifedipine, and triclocarban exceeded 0.1, indicating potential moderate or high risks. Antibacterial agents in daily chemicals and azole broad-spectrum antifungals were associated with the highest risks in this study; this might be another significant pollution characteristic in the sea area around this subtropical tourist city.
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http://dx.doi.org/10.1007/s11356-020-12335-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797026PMC
January 2021

Noninferiority of cetuximab every-2-weeks versus standard once-weekly administration schedule for the first-line treatment of RAS wild-type metastatic colorectal cancer.

Eur J Cancer 2021 Feb 28;144:291-301. Epub 2020 Dec 28.

Department of Medical Oncology, Internal Medicine 1, General Hospital - Medical University of Vienna, Vienna, Austria.

Aim: This study assessed whether cetuximab 500 mg/m administered every 2 weeks (Q2W), when combined with chemotherapy as a first-line (1L) treatment, was noninferior to the approved dose (400 mg/m followed by 250 mg/m once weekly [Q1W]) for overall survival (OS) in adults with RAS wild-type metastatic colorectal cancer (mCRC).

Methods: This pooled analysis included patients receiving 1L treatment with cetuximab Q1W or Q2W in combination with chemotherapy from post-authorisation studies with patient-level data available to the sponsor. Baseline characteristics were adjusted with a propensity score using inverse probability of treatment weighting (IPTW). Noninferiority in terms of OS was tested with a noninferiority margin for the hazard ratio (HR) of 1.25 using a Cox proportional hazards regression model. Secondary outcomes were progression-free survival (PFS), overall response rate (ORR) and rates of lung/liver metastases resection and serious adverse events.

Results: OS time was noninferior in the Q2W cohort (n = 554) compared to the Q1W cohort (n = 763), with a HR after IPTW (95% confidence interval) of 0.827 (0.715-0.956) and median OS times of 24.7 (Q1W) and 27.9 (Q2W) months. There were no major differences in PFS (HR: 0.915 [0.804-1.042]). The odds ratios (ORs) after IPTW for ORR (1.292 [1.031-1.617]) and the rates of lung/liver metastases resection (1.419 [1.043-1.932]) favoured the Q2W regimen. No differences were noted in the occurrence rate of any SAE between groups; the OR after IPTW was 1.089 (0.858-1.382).

Conclusions: The cetuximab Q2W regimen was noninferior to the Q1W regimen for OS in the 1L treatment of mCRC.
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http://dx.doi.org/10.1016/j.ejca.2020.11.013DOI Listing
February 2021

Extended RAS Analysis of the Phase III EPIC Trial: Irinotecan + Cetuximab Versus Irinotecan as Second-Line Treatment for Patients with Metastatic Colorectal Cancer.

Oncologist 2021 02 14;26(2):e261-e269. Epub 2020 Dec 14.

Tennessee Oncology Sarah Cannon Research Institute, Nashville, Tennessee, USA.

Background: The multicenter, open-label, randomized, phase III EPIC study (EMR 062202-025) investigated cetuximab plus irinotecan versus irinotecan in patients with epidermal growth factor receptor-detectable metastatic colorectal cancer (mCRC) that progressed on first-line fluoropyrimidine- and oxaliplatin-based chemotherapy; we report the outcomes of patients with RAS-wild-type (wt) disease.

Materials And Methods: Available DNA samples from RAS-unselected patients (n = 1,164 of 1,298 [89.7%]) were reanalyzed for RAS mutations using beads, emulsion, amplification, and magnetics. Baseline characteristics, efficacy, safety, and poststudy therapy were assessed. RAS-wt status was defined as a mutated RAS allele frequency of ≤5%, with all relevant alleles being analyzable.

Results: Baseline characteristics were comparable between the groups (n = 452 patients with RAS-wt mCRC; cetuximab plus irinotecan n = 231, irinotecan n = 221) and between the RAS-wt and RAS-unselected populations. In the cetuximab plus irinotecan versus irinotecan arms, median overall survival was 12.3 versus 12.0 months, median progression-free survival (PFS) was 5.4 versus 2.6 months, and objective response rate (ORR) was 29.4% versus 5.0%, respectively. Quality of life (QoL) was improved in the cetuximab plus irinotecan arm. Serious adverse events occurred in 45.4% (cetuximab plus irinotecan) and 42.4% (irinotecan) of patients. In total, 47.1% of patients in the irinotecan arm received subsequent cetuximab therapy.

Conclusion: PFS, ORR, and QoL were improved with cetuximab plus irinotecan as a second-line treatment in patients with RAS-wt mCRC, confirming that cetuximab-based therapy is suitable in this population. Almost half of patients in the irinotecan arm received poststudy cetuximab, masking a potential overall survival benefit of cetuximab addition.

Implications For Practice: Cetuximab is approved for the treatment of RAS-wild-type metastatic colorectal cancer (mCRC). In this retrospective analysis of the phase III EPIC study (cetuximab plus irinotecan vs. irinotecan alone as second-line treatment in patients with RAS-unselected mCRC), the subgroup of patients with RAS-wild-type mCRC who received cetuximab plus irinotecan had improved progression-free survival, objective response rate, and quality of life compared with the RAS-unselected population. These findings suggest that cetuximab-based therapy is a suitable second-line treatment for patients with RAS-wild-type mCRC.
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http://dx.doi.org/10.1002/onco.13591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873334PMC
February 2021

Paradoxical Mitophagy Regulation by PINK1 and TUFm.

Mol Cell 2020 11 27;80(4):607-620.e12. Epub 2020 Oct 27.

School of Life Sciences, Tianjin University, Tianjin 300072, China.

Aberrant mitophagy has been implicated in a broad spectrum of disorders. PINK1, Parkin, and ubiquitin have pivotal roles in priming mitophagy. However, the entire regulatory landscape and the precise control mechanisms of mitophagy remain to be elucidated. Here, we uncover fundamental mitophagy regulation involving PINK1 and a non-canonical role of the mitochondrial Tu translation elongation factor (TUFm). The mitochondrion-cytosol dual-localized TUFm interacts with PINK1 biochemically and genetically, which is an evolutionarily conserved Parkin-independent route toward mitophagy. A PINK1-dependent TUFm phosphoswitch at Ser222 determines conversion from activating to suppressing mitophagy. PINK1 modulates differential translocation of TUFm because p-S222-TUFm is restricted predominantly to the cytosol, where it inhibits mitophagy by impeding Atg5-Atg12 formation. The self-antagonizing feature of PINK1/TUFm is critical for the robustness of mitophagy regulation, achieved by the unique kinetic parameters of p-S222-TUFm, p-S65-ubiquitin, and their common kinase PINK1. Our findings provide new mechanistic insights into mitophagy and mitophagy-associated disorders.
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http://dx.doi.org/10.1016/j.molcel.2020.10.007DOI Listing
November 2020

Mesorhizobium jarvisii is a dominant and widespread species symbiotically efficient on Astragalus sinicus L. in the Southwest of China.

Syst Appl Microbiol 2020 Sep 13;43(5):126102. Epub 2020 Jun 13.

State Key Laboratory of Agrobiotechnology, Beijing 100193, PR China; College of Biological Sciences and Rhizobium Research Center, China Agricultural University, Beijing, 100193, PR China.

In order to identify rhizobia of Astragalus sinicus L. and estimate their geographic distribution in the Southwest China, native rhizobia nodulating A. sinicus were isolated and their genetic diversity were studied at 13 sites cultivated in four Chinese provinces. A total of 451 rhizobial isolates were trapped with A. sinicus plants from soils and classified into 8 different genotypes defined by PCR-based restriction fragment length polymorphism (RFLP) of 16S-23S rRNA intergenic spacer (IGS). Twenty-one representative strains were further identified into three defined Mesorhizobium species by phylogenetic analyses of 16S rRNA genes and housekeeping genes (glnII and atpD). M. jarvisii was dominant accounting for 76.3% of the total isolates, 22.8% of the isolates were identified as M. huakuii and five strains belonged to M. qingshengii. All representatives were assigned to the symbiovar astragali by sharing high nodC sequence similarities of more than 99%. Furthermore, the biogeography distribution of these rhizobial genotypes and species was mainly affected by contents of available phosphorus, available potassium, total salts and pH in soils. The most remarkable point was the identification of M. jarvisii as a widespread and predominant species of A. sinicus in southwest of China. These results revealed a novel geographic pattern of rhizobia associated with A. sinicus in China.
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http://dx.doi.org/10.1016/j.syapm.2020.126102DOI Listing
September 2020

MicroRNA-146b correlates with decreased acute respiratory distress syndrome risk, reduced disease severity, and lower 28-day mortality in sepsis patients.

J Clin Lab Anal 2020 Dec 26;34(12):e23510. Epub 2020 Aug 26.

Department of Intensive Care Unit, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: This study aimed to investigate the predictive value of microRNA-146b (miR-146b) on acute respiratory distress syndrome (ARDS) risk, and the correlation of miR-146b with disease severity and 28-day mortality in sepsis patients.

Methods: A total of 104 sepsis patients and 100 healthy controls (HCs) were consecutively enrolled, and miR-146b relative expression in their plasma samples was detected by reverse transcription-quantitative polymerase chain reaction. In sepsis patients, disease severity was assessed using Acute Physiology and Chronic Health Evaluation II (APACHE II) score and Sequential Organ Failure Assessment (SOFA) score. ARDS occurrence and 28-day mortality were recorded.

Results: MiR-146b was decreased in sepsis patients compared to HCs. ARDS occurred in 30 (28.8%) sepsis patients, and miR-146b was reduced in ARDS sepsis patients compared to non-ARDS sepsis patients. Meanwhile, miR-146b distinguished ARDS sepsis patients from non-ARDS sepsis patients (area under the curve (AUC): 0.728, 95% confidence interval (CI): 0.627-0.829). Subsequent multivariate logistic regression showed that miR-146b, age, smoke, respiratory infection, and serum creatinine predicted ARDS risk independently, and their combination well-discriminated ARDS sepsis patients from non-ARDS sepsis patients (AUC: 0.863, 95% CI: 0.792-0.934). Additionally, miR-146b was negatively correlated with serum creatinine, white blood cell, C-reactive protein, APACHE II score, and SOFA score, while positively correlated with albumin. Regarding prognosis, miR-146b was decreased in 28-day sepsis deaths compared to 28-day sepsis survivors, and it discriminated 28-day sepsis deaths from 28-day sepsis survivors (AUC: 0.785, 95% CI: 0.680-0.890).

Conclusion: MiR-146b might serve as a potential biomarker for ARDS prevention and prognostic reflection in sepsis.
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http://dx.doi.org/10.1002/jcla.23510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755760PMC
December 2020

Comparative efficacy of social media delivered health education on glycemic control: A meta-analysis.

Int J Nurs Sci 2020 Jul 28;7(3):359-368. Epub 2020 May 28.

Department of Family Health Care Nursing, University of California San Francisco, San Francisco, CA, United States.

Objective: To compare outcomes associated with patient education about glycemic control via group chat versus patient education as usual among individuals with diabetes in China.

Methods: We searched the following databases both in English and in Chinese languages: PubMed, CNKI, Wanfang database, VIP database, and CBM for articles published up to Jan 1, 2018. The studies were screened by two independent reviewers. Using criteria from the risk of bias assessment tool developed by Cochrane Collaboration to assess the risk of bias of eligible studies. A meta-analysis of studies was performed using comprehensive meta-analysis version 3.0.

Results: Twenty-five unique randomized clinical trials, including 2,838 patients, were identified. The education delivered via group chat had large overall pooled effect sizes in improving glucose control measured by hemoglobin A1c [Hedges' g = -0.81, 95% CI: (-0.98, -0.64)], fasting blood glucose [Hedges' g = -1.11, 95% CI: (-1.37, -0.85)], and 2 h postprandial blood glucose [Hedges' g = -0.98, 95% CI: (-1.20, -0.76)]. Additionally, patient education delivered via group chat has shown consistently superior outcomes in glucose control in short-term (0-3 months), mid-term (3-6 months) and longer-term (6-12 months).

Conclusions: Educational interventions via group chat had a superior outcome in blood glucose control compared to education as usual in China. Educational interventions via group chat had superior short-term, mid-term, and longer-term outcomes in blood glucose control compared to education as usual in China.
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http://dx.doi.org/10.1016/j.ijnss.2020.04.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7424160PMC
July 2020

Perceptual Advantage of Animal Facial Attractiveness: Evidence From b-CFS and Binocular Rivalry.

Front Psychol 2020 10;11:1670. Epub 2020 Jul 10.

Department of Psychology, Bournemouth University, Poole, United Kingdom.

Research has shown that attractive human faces enjoy an advantage in both conscious and preconscious processing. Here we examined whether this preference for attractiveness is exclusive to human faces by measuring participants' sensitivity to the attractiveness of cat and tiger faces. Experiment 1 measured the time taken to break continuous flash suppression (b-CFS), whereas Experiment 2 measured the dominant time in binocular rivalry (BR). The results showed that attractive cat faces were detected more quickly (Experiment 1) and dominated for longer time in visual awareness (Experiment 2). However, no effect of attractiveness was found for tiger faces in Experiment 1, while attractive tiger faces also dominated for longer time in visual awareness in Experiment 2. The results provide first evidence that the preference for attractive animal faces can be shown involuntarily or without apparent conscious control. The findings suggest that human preference for facial attractiveness may contain an aesthetic element rather than being a purely adaptive means for mate choice.
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http://dx.doi.org/10.3389/fpsyg.2020.01670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367084PMC
July 2020

Attenuation of epigenetic regulator SMARCA4 and ERK-ETS signaling suppresses aging-related dopaminergic degeneration.

Aging Cell 2020 09 4;19(9):e13210. Epub 2020 Aug 4.

Institute of Life Sciences, Fuzhou University, Fuzhou, Fujian, China.

How complex interactions of genetic, environmental factors and aging jointly contribute to dopaminergic degeneration in Parkinson's disease (PD) is largely unclear. Here, we applied frequent gene co-expression analysis on human patient substantia nigra-specific microarray datasets to identify potential novel disease-related genes. In vivo Drosophila studies validated two of 32 candidate genes, a chromatin-remodeling factor SMARCA4 and a biliverdin reductase BLVRA. Inhibition of SMARCA4 was able to prevent aging-dependent dopaminergic degeneration not only caused by overexpression of BLVRA but also in four most common Drosophila PD models. Furthermore, down-regulation of SMARCA4 specifically in the dopaminergic neurons prevented shortening of life span caused by α-synuclein and LRRK2. Mechanistically, aberrant SMARCA4 and BLVRA converged on elevated ERK-ETS activity, attenuation of which by either genetic or pharmacological manipulation effectively suppressed dopaminergic degeneration in Drosophila in vivo. Down-regulation of SMARCA4 or drug inhibition of MEK/ERK also mitigated mitochondrial defects in PINK1 (a PD-associated gene)-deficient human cells. Our findings underscore the important role of epigenetic regulators and implicate a common signaling axis for therapeutic intervention in normal aging and a broad range of age-related disorders including PD.
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http://dx.doi.org/10.1111/acel.13210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511865PMC
September 2020

Genomic diversity of chickpea-nodulating rhizobia in Ningxia (north central China) and gene flow within symbiotic Mesorhizobium muleiense populations.

Syst Appl Microbiol 2020 Jul 20;43(4):126089. Epub 2020 May 20.

The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.

Diversity and taxonomic affiliation of chickpea rhizobia were investigated from Ningxia in north central China and their genomic relationships were compared with those from northwestern adjacent regions (Gansu and Xinjiang). Rhizobia were isolated from root-nodules after trapping by chickpea grown in soils from a single site of Ningxia and typed by IGS PCR-RFLP. Representative strains were phylogenetically analyzed on the basis of the 16S rRNA, housekeeping (atpD, recA and glnII) and symbiosis (nodC and nifH) genes. Genetic differentiation and gene flow were estimated among the chickpea microsymbionts from Ningxia, Gansu and Xinjiang. Fifty chickpea rhizobial isolates were obtained and identified as Mesorhizobium muleiense. Their symbiosis genes nodC and nifH were highly similar (98.4 to 100%) to those of other chickpea microsymbionts, except for one representative strain (NG24) that showed low nifH similarities with all the defined Mesorhizobium species. The rhizobial population from Ningxia was genetically similar to that from Gansu, but different from that in Xinjiang as shown by high chromosomal gene flow/low differentiation with the Gansu population but the reverse with the Xinjiang population. This reveals a biogeographic pattern with two main populations in M. muleiense, the Xinjiang population being chromosomally differentiated from Ningxia-Gansu one. M. muleiense was found as the sole main chickpea-nodulating rhizobial symbiont of Ningxia and it was also found in Gansu sharing alkaline-saline soils with Ningxia. Introduction of chickpea in recently cultivated areas in China seems to select from alkaline-saline soils of M. muleiense that acquired symbiotic genes from symbiovar ciceri.
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http://dx.doi.org/10.1016/j.syapm.2020.126089DOI Listing
July 2020

Response of glacial lakes to glacier and climate changes in the western Nyainqentanglha range.

Sci Total Environ 2020 Sep 22;735:139607. Epub 2020 May 22.

Key Laboratory of Tibetan Environmental Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.

The western Nyainqentanglha (WNT) range, located in the transition zone between large-scale atmospheric circulations, has an abundance of glacial lakes and glaciers. In the warmer climate of recent decades, the glacial lakes and glaciers in the WNT range could have changed substantially. Here, glacial lake and glacier changes for the entire WNT range between 1976 and 2018 are examined. The results show that, between 1976 and 2018, the number of glacial lakes (>0.0036 km) increased by 56% from 192 to 299 and their total area increased by 35% from 6.75 ± 0.13 km to 9.12 ± 0.13 km. The glacial lakes expanded faster in 2001-2018 (0.08 km/yr) than in 1976-2001 (0.04 km/yr), and faster on the southern side of the mountain range than on the northern side. Correspondingly, the rates of glacier area shrinkage and surface elevation thinning between 2000 and 2018 were -4.15 km/yr and -0.29 ± 0.06 m/yr, respectively, greater than the respective rates of -3.91 km/yr and -0.24 ± 0.11 m/yr in 1976-2000. The glacier retreat and elevation thinning to the south of the main divide are greater than to the north, with the exception of the thinning rate in 2000-2014 which is possibly due to precipitation differences. With the rapid retreat of glaciers, many glacial lakes are changing from proglacial lakes to unconnected glacial lakes. The increases in the number and area of glacial lakes are moving toward higher elevations, accompanying the retreat of glaciers and the increase in the mean elevation of glaciers. The observed increased precipitation also supports the increases of glacial lakes. The accelerating glacier loss and lake expansion in the WNT range imply a strong impact of climate change on the cryosphere over the past few decades.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139607DOI Listing
September 2020

Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean.

ISME J 2020 08 27;14(8):1915-1928. Epub 2020 Apr 27.

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Road, Hongshan District, Wuhan, 430070, Hubei, China.

Rhizobia-legume symbiosis is an important type of plant-microbe mutualism; however, the establishment of this association is complicated and can be affected by many factors. The soybean rhizosphere has a specific microbial community, yet whether these organisms affect rhizobial nodulation has not been well investigated. Here, we analyzed the compositions and relationships of soybean rhizocompartment microbiota in three types of soil. First, we found that the rhizosphere community composition of soybean varied significantly in different soils, and the association network between rhizobia and other rhizosphere bacteria was examined. Second, we found that some rhizosphere microbes were correlated with the composition of bradyrhizobia and sinorhizobia in nodules. We cultivated 278 candidate Bacillus isolates from alkaline soil. Finally, interaction and nodulation assays showed that the Bacillus cereus group specifically promotes and suppresses the growth of sinorhizobia and bradyrhizobia, respectively, and alleviates the effects of saline-alkali conditions on the nodulation of sinorhizobia as well as affecting its colonization in nodules. Our findings demonstrate a crucial role of the bacterial microbiota in shaping rhizobia-host interactions in soybean, and provide a framework for improving the symbiotic efficiency of this system of mutualism through the use of synthetic bacterial communities.
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http://dx.doi.org/10.1038/s41396-020-0648-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367843PMC
August 2020

Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species.

Nucleic Acids Res 2020 06;48(10):e57

State Key Laboratory of Biomembrane and Membrane Engineering, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.

Site-specific DNA double-strand breaks have been used to generate knock-in through the homology-dependent or -independent pathway. However, low efficiency and accompanying negative impacts such as undesirable indels or tumorigenic potential remain problematic. In this study, we present an enhanced reduced-risk genome editing strategy we named as NEO, which used either site-specific trans or cis double-nicking facilitated by four bacterial recombination factors (RecOFAR). In comparison to currently available approaches, NEO achieved higher knock-in (KI) germline transmission frequency (improving from zero to up to 10% efficiency with an average of 5-fold improvement for 8 loci) and 'cleaner' knock-in of long DNA fragments (up to 5.5 kb) into a variety of genome regions in zebrafish, mice and rats. Furthermore, NEO yielded up to 50% knock-in in monkey embryos and 20% relative integration efficiency in non-dividing primary human peripheral blood lymphocytes (hPBLCs). Remarkably, both on-target and off-target indels were effectively suppressed by NEO. NEO may also be used to introduce low-risk unrestricted point mutations effectively and precisely. Therefore, by balancing efficiency with safety and quality, the NEO method reported here shows substantial potential and improves the in vivo gene-editing strategies that have recently been developed.
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http://dx.doi.org/10.1093/nar/gkaa195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261186PMC
June 2020

Are China's water bodies (lakes) underestimated?

Proc Natl Acad Sci U S A 2020 03 3;117(12):6308-6309. Epub 2020 Mar 3.

Division of Geodetic Science, School of Earth Sciences, The Ohio State University, Columbus, OH 43210.

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http://dx.doi.org/10.1073/pnas.1922250117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104340PMC
March 2020

Optimizing pH-sensitive and time-dependent polymer formula of colonic pH-responsive pellets to achieve precise drug release.

Asian J Pharm Sci 2019 Jul 28;14(4):413-422. Epub 2018 Aug 28.

School of Pharmacy, Guangdong Medical University, No.1, Xincheng Road, Dongguan 523808, China.

Time-sensitive and pH-dependent polymers are generally employed to prepare colon-site delivery system, and their coating thickness and order are very important in controlling the drug release. The traditional colon-site delivery systems consist of time-dependent polymers as inner layer and pH-sensitive polymers as outer layer. However, they suffer from low drug-loading rate and immature drug release. In this study, total alkaloids of sophora alopecuroides(TASA)-loaded pellets were prepared by extrusion-spheronization method and coated with Eudragit RS30D and Eudragit S100. Pellets using Eudragit RS30D as inner layer and Eudragit S100 as outer layer were named as ERS-ES100 TCO, while pellets with Eudragit S100 as inner layer and Eudragit RS30D as outer layer were ES100-ERS NCO. Both types of formulations with varying coating ratios and orders of Eudragit S100 and Eudragit RS30D were designed and prepared. The following drug release and SEM studies indicated that ERS-ES100 TCO(F2) with 12.8% Eudragit RS30D as inner layer and 21% Eudragit S100 as outer layer released up to 42% drug in 5 h. Interestingly, ES100-ERS NCO (F4) coated with 12.8% Eudragit S100 and 14.8% Eudragit RS30D showed optimal drug release in colon. In conclusion, ES100-ERS NCO colonic delivery system achieved reduced coating thickness and improved colonic targeting compared with traditional delivery system (ERS-ES100 TCO). In addition, the similarity factors ( ) value of sophoridine and matrine for investigated formulation were within 50-100 and > 80, demonstrating that sophoridine and matrine in all formulations achieved a synchronous release.
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http://dx.doi.org/10.1016/j.ajps.2018.05.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7032081PMC
July 2019

Perceptual integration and the composite face effect.

Q J Exp Psychol (Hove) 2020 Jul 30;73(7):1101-1114. Epub 2020 Jan 30.

Department of Psychology, Renmin University of China, Beijing, P.R. China.

The composite face paradigm is widely used to investigate holistic perception of faces. In the paradigm, parts from different faces (usually the top and bottom halves) are recombined. The principal criterion for holistic perception is that responses involving the component parts of composites in which the parts are aligned into a face-like configuration are disrupted compared with the same parts in a misaligned (not face-like) format. This is often taken as evidence that seeing a whole face in the aligned condition interferes with perceiving its separate parts, but the extent to which the effect is perceptually driven remains unclear. We used salient perceptual categories of gender (male or female) and race (Asian or Caucasian appearance) to create composite stimuli from parts of faces that varied orthogonally on these characteristics. In Experiment 1, participants categorised the gender of the parts of aligned composite and misaligned images created from parts with the same (congruent) or different (incongruent) gender and the same (congruent) or different (incongruent) race. In Experiment 2, the same stimuli were used but the task changed to categorising race. In both experiments, there was a strong influence of the task-relevant manipulation on the composite effect, with slower responses to aligned stimuli with incongruent gender in Experiment 1 and incongruent race in Experiment 2. In contrast, the task-irrelevant variable (race in Experiment 1, gender in Experiment 2) did not exert much influence on the composite effect in either experiment. These findings show that although holistic integration of salient visual properties makes a strong contribution to the composite face effect, it clearly also involves targeted processing of an attended visual characteristic.
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http://dx.doi.org/10.1177/1747021819899531DOI Listing
July 2020

High-Salt Diet Causes Sleep Fragmentation in Young Through Circadian Rhythm and Dopaminergic Systems.

Front Neurosci 2019 29;13:1271. Epub 2019 Nov 29.

Institute of Life Sciences, College of Biological Science and Engineering, Fuzhou University, Fuzhou, China.

Salt (sodium chloride) is an essential dietary requirement, but excessive consumption has long-term adverse consequences. A high-salt diet (HSD) increases the risk of chronic diseases such as cardiovascular conditions and diabetes and is also associated with poor sleep quality. Little is known, however, about the neural circuit mechanisms that mediate HSD-induced sleep changes. In this study, we sought to identify the effects of HSD on the sleep and related neural circuit mechanisms of . Strikingly, we found that HSD causes young to exhibit a fragmented sleep phenotype similar to that of normal aging individuals. Importantly, we further showed that HSD slightly impairs circadian rhythms and that the HSD-induced sleep changes are dependent on the circadian rhythm system. In addition, we demonstrated that HSD-induced sleep changes are dopaminergic-system dependent. Together, these results provide insight into how elevated salt in the diet can affect sleep quality.
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http://dx.doi.org/10.3389/fnins.2019.01271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895215PMC
November 2019

The Role of Gender in the Preconscious Processing of Facial Trustworthiness and Dominance.

Front Psychol 2019 15;10:2565. Epub 2019 Nov 15.

Department of Psychology, Renmin University of China, Beijing, China.

The present study adopted the breaking continuous flash suppression paradigm (b-CFS) to investigate how Chinese participants process trustworthiness (Experiment 1) and dominance (Experiment 2) at the preconscious level. In addition, we tested whether the gender of a face and the gender of a participant can influence the preconscious processing of facial trustworthiness and dominance. Experiment 1 showed that the least and most trustworthy faces both took significantly less time to break into awareness than neutral faces. In Experiment 2, for female faces, neutral faces took significantly less time to break into awareness than the least and most dominant faces. In both experiments, female faces broke through suppression faster than male faces. In summary, for Chinese participants, the preconscious processing of trustworthiness was not different between male and female faces. However, the preconscious processing of dominance was different between male and female faces.
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http://dx.doi.org/10.3389/fpsyg.2019.02565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6872516PMC
November 2019

Monitoring Differential Subsidence along the Beijing-Tianjin Intercity Railway with Multiband SAR Data.

Int J Environ Res Public Health 2019 11 13;16(22). Epub 2019 Nov 13.

Beijing Institute of Hydrogeology and Engineering Geology, Beijing 100195, China.

High-speed railways have strict standards of infrastructure deformation and post-construction settlement. The interferometric synthetic aperture radar (InSAR) has the ability to detect ground deformation with a high accuracy and wide coverage and is becoming a useful tool for monitoring railway health. In this study, we analyzed the Beijing-Tianjin Intercity Railway (BTIR) track using InSAR time-series analysis with different data sets. First, by using RADARSAT-2 images, we examined the areas along the BTIR with significant subsidence. Then, we characterized these areas by means of X-band TerraSAR-X data. We adopted the expectation (Ex) and entropy (En) method, combined with GIS spatial analysis, to analyze the ground settlement differences on both sides of the railway. The results show that the area with the most severe differential settlement occurs between 12 and 20 km along the railway and within 120 to 20 m on both sides of the Chaoyang-Tongzhou section (CTS). Thereafter, we analyzed the reasons for the large difference in this area by considering different factors, e.g., regional land subsidence, groundwater level changes, and the dynamic load. In addition, we studied the impact of regional subsidence on the safe operation of the BTIR. The results show that the maximum different settlement along the BTIR is within the safe range, according to the high-speed railway design standard between 2010 and 2015. This study aims to provide technical support for assessing the impact of subsidence on the safety of railway operations.
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http://dx.doi.org/10.3390/ijerph16224453DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888550PMC
November 2019

miR-263b Controls Circadian Behavior and the Structural Plasticity of Pacemaker Neurons by Regulating the LIM-Only Protein Beadex.

Cells 2019 08 18;8(8). Epub 2019 Aug 18.

Department of Biology, University of Nevada Reno, Reno, NV 89557, USA.

Circadian clocks drive rhythmic physiology and behavior to allow adaption to daily environmental changes. In , the small ventral lateral neurons (sLNvs) are primary pacemakers that control circadian rhythms. Circadian changes are observed in the dorsal axonal projections of the sLNvs, but their physiological importance and the underlying mechanism are unclear. Here, we identified as an important regulator of circadian rhythms and structural plasticity of sLNvs in . Depletion of () in flies dramatically impaired locomotor rhythms under constant darkness. Indeed, is required for the structural plasticity of sLNvs. regulates circadian rhythms through inhibition of expression of the LIM-only protein Consistently, overexpression of or loss-of-function mutation () phenocopied and overexpression in behavior and molecular characteristics. In addition, mutating the binding sites in the 3' UTR using CRISPR/Cas9 recapitulated the circadian phenotypes of flies. Together, these results establish as an important regulator of circadian locomotor behavior and structural plasticity.
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http://dx.doi.org/10.3390/cells8080923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721658PMC
August 2019

ATP1A1 mutations cause intermediate Charcot-Marie-Tooth disease.

Hum Mutat 2019 12 23;40(12):2334-2343. Epub 2019 Aug 23.

Department of Neurology and Institute of Neurology, First Affiliated Hospital, Fujian Medical University, Fuzhou, China.

Intermediate Charcot-Marie-Tooth (CMT) disease is a heterogeneous group of inherited neuropathies characterized by progressive muscle weakness and atrophy of the distal extremities, distal sensory loss. There were still a large proportion of causative genes for intermediate CMT failed to be identified. Here, using whole-exome sequencing technique, we identified two novel missense mutations in ATP1A1 gene, c.620C>T (p.S207F) and c.2629G>A (p.G877S), in two Chinese CMT families. Further functional analysis revealed that these mutations led to the loss function of the ATP1A1 protein. The two mutations did not affect the levels of messenger RNA but possessed a damaging effect on ATP1A1 protein expression and they downregulated the protein levels of ATP1A1 by promoting its proteasome degradation. Taken together, we confirmed ATP1A1 as a novel causative gene for intermediate CMT.
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http://dx.doi.org/10.1002/humu.23886DOI Listing
December 2019

Editorial: Recognizing Microexpression: An Interdisciplinary Perspective.

Front Psychol 2019 4;10:1318. Epub 2019 Jun 4.

Department of Psychology, Renmin University of China, Beijing, China.

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http://dx.doi.org/10.3389/fpsyg.2019.01318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558206PMC
June 2019

Self-Construal Priming Modulates Ensemble Perception of Multiple-Face Identities.

Front Psychol 2019 16;10:1096. Epub 2019 May 16.

Department of Psychology, Renmin University of China, Beijing, China.

This study explored the modulatory role of independent/interdependent self-construal on ensemble perception. Two experiments were conducted to study the effect of self-construal on ensemble coding of multiple-face identities (Experiment 1) and dot size (Experiment 2) separately. Before the implicit ensemble perception task, participants in both experiments were either primed with independent or interdependent self-construal a well-validated pronoun circle task, in which they were exposed to either singular ("," "," and "") or plural ("," "," and "") pronouns in essays. The results showed that interdependent self-construal (vs. independent self-construal) featured as global processing and emphasizing interconnectedness with others enhanced the ensemble coding of high-level features (e.g., identity in Experiment 1) but not of low-level features (e.g., size in Experiment 2). To the best of our knowledge, this study was the first to investigate the role of self-construal on ensemble representations. In sum, the results of the current study supported the domain-specific mechanism of ensemble perception on one hand, and extended the effect of self-construal on single face recognition to multiple face recognition on the other hand.
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http://dx.doi.org/10.3389/fpsyg.2019.01096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532433PMC
May 2019

Phase II APEC trial: The impact of primary tumor side on outcomes of first-line cetuximab plus FOLFOX or FOLFIRI in patients with RAS wild-type metastatic colorectal cancer.

Asia Pac J Clin Oncol 2019 Aug 15;15(4):225-230. Epub 2019 May 15.

National Taiwan University Hospital, Taipei, Taiwan.

Aim: The open-label, nonrandomized, phase II APEC study enrolled 167 patients with RAS wild-type (wt) metastatic colorectal cancer (mCRC) to investigate the safety and efficacy of first-line, every-2-weeks cetuximab plus investigator's choice of FOLFIRI or FOLFOX in this patient population.

Methods: A subgroup analysis of the APEC study population by primary tumor location was performed.

Results: A total of 130 patients (81.8%) had left-sided and 29 (18.2%) had right-sided mCRC. Median progression-free survival (PFS), overall survival (OS) and overall response rate (ORR) were 14.0 months, 30.6 months and 68.5% for patients with left-sided tumors and 8.9 months, 24.6 months and 51.7% for patients with right-sided mCRC, concurring with pivotal phase III trial results. In patients with right-sided tumors, median PFS was 15.4 months vs 8.3 months with cetuximab plus FOLFIRI vs cetuximab plus FOLFOX, respectively; median OS was 32.1 months vs 21.8 months with cetuximab plus FOLFIRI vs cetuximab plus FOLFOX, respectively.

Conclusion: The APEC tumor-location subgroup analysis results were largely consistent with available literature regarding the equivalent efficacy of cetuximab plus FOLFIRI/FOLFOX in patients with left-sided RAS wt mCRC. A trend toward improved efficacy with cetuximab plus FOLFIRI compared with cetuximab plus FOLFOX was observed in patients with right-sided tumors; however, a direct comparison between groups cannot be made due to the nonrandomized study design. Nevertheless, the similar ORR observed with either chemotherapy backbone in patients with right-sided RAS wt mCRC suggests a potential role for both regimens in this patient population when cytoreduction is a treatment goal.
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http://dx.doi.org/10.1111/ajco.13154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852115PMC
August 2019

Loss of in Circadian Cells Decreases the Amplitude of the Circadian Locomotor Rhythm in .

Front Cell Neurosci 2019 1;13:76. Epub 2019 Mar 1.

Department of Biology, University of Nevada, Reno, Reno, NV, United States.

The circadian system, which has a period of about 24 h, is import for organismal health and fitness. The molecular circadian clock consists of feedback loops involving both transcription and translation, and proper function of the circadian system also requires communication among intracellular organelles. As important hubs for signaling in the cell, mitochondria integrate a variety of signals. Mitochondrial dysfunction and disruption of circadian rhythms are observed in neurodegenerative diseases and during aging. However, how mitochondrial dysfunction influences circadian rhythm is largely unknown. Here, we report that , which localizes to the mitochondrial matrix, most likely affects the function of certain clock neurons.Deletion of in flies caused decreased expression of mitochondrial transcription factor TFAM and reductions in levels of mitochondrial DNA, which resulted in mitochondrial dysfunction. Loss of decreased the amplitude of circadian rhythms.In addition, we showed that depletion of mtDNA by overexpression of a mitochondrially targeted restriction enzyme mitoXhoI also decreased the robustness of circadian rhythms. Our work demonstrates that is important for mitochondrial function thus involved in the regulation of circadian rhythms.
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http://dx.doi.org/10.3389/fncel.2019.00076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405476PMC
March 2019

Identification and Characterization of Three New Cytochrome P450 Genes and the Use of RNA Interference to Evaluate Their Roles in Antioxidant Defense in Fabricius.

Front Physiol 2018 15;9:1608. Epub 2018 Nov 15.

College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.

Cytochrome P450s play critical roles in maintaining redox homeostasis and protecting organisms from the accumulation of toxic reactive oxygen species (ROS). The biochemical functions of the P450 family have essentially been associated with the metabolism of xenobiotics. Here, we sequenced and characterized three P450 genes, , and , from Fabricius; these genes play a critical role in maintaining biodiversity. Quantitative PCR (qPCR) analysis indicated that the three genes were all predominantly expressed in the epidermis (EP), followed by the brain (BR) and midgut (MG). In addition, the highest expression levels were detected in the dark-eyed pupae and adult stages. The three genes were induced by temperature (4°C and 44°C), heavy metals (CdCl and HgCl), pesticides (DDV, deltamethrin, and paraquat) and UV treatments. Furthermore, Western blot analysis indicated that the protein expression levels could be induced by some abiotic stressors, a result that complements the qPCR results. We analyzed the silencing of these three genes and found that silencing these genes enhanced the enzymatic activities of peroxidase (POD) and catalase (CAT). Additionally, we investigated the expression of other antioxidant genes and found that some were upregulated, while others were downregulated, suggesting that the upregulated genes may be involved in compensating for the silencing of , and . Our findings suggest that , and may play very significant roles in the antioxidant defense against damage caused by ROS.
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http://dx.doi.org/10.3389/fphys.2018.01608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250095PMC
November 2018

Genetic divergence among Bradyrhizobium strains nodulating wild and cultivated Kummerowia spp. in China.

Syst Appl Microbiol 2019 Mar 12;42(2):223-231. Epub 2018 Oct 12.

State Key Laboratory of Agrobiotechnology, Beijing 100193, China; College of Biological Sciences and Rhizobium Research Center, China Agricultural University, Beijing 100193, China. Electronic address:

Distribution of rhizobial species is affected by geographical isolation and selected by leguminous hosts, however, little is known about the molecular evolution of rhizobia nodulating the same legume in different eco-environments. In present study, the microevolution of Bradyrhizobium associated with the leguminous grass Kummerowia grown in exurban areas and cultivated in urban areas in China was investigated. Total 14 genospecies, including seven new groups, were identified based on a concatenated sequence analysis of taxonomic markers (SMc00019, truA and thrA) for 94 representative strains. Results demonstrated that lower levels of nucleotide diversity were found in the strains isolated from urban areas compared with those isolated from exurban areas, based on the evolutional analyses of three housekeeping genes (atpD, glnII and recA), two symbiosis-related genes (nodC and nifH), and the taxonomic markers. Moreover, compared with urban areas, gene exchange and recombination occurred more frequently among the genospecies isolated from exurban areas, regardless of the geographical distribution. Finally, the evolutionary lineage of Bradyrhizobium strains isolated from urban areas was independent of that of the strains isolated from exurban areas. In summary, the evolutionary history of Kummerowia bradyrhizobia may have been gradually segregated to different evolutionary lineages, irrespective of distinct biogeography.
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http://dx.doi.org/10.1016/j.syapm.2018.10.003DOI Listing
March 2019

The auxin-inducible degradation system enables conditional PERIOD protein depletion in the nervous system of Drosophila melanogaster.

FEBS J 2018 12 25;285(23):4378-4393. Epub 2018 Oct 25.

Department of Biology, University of Nevada Reno, NV, USA.

Tools that allow inducible and reversible depletion of target proteins are critical for biological studies. The plant-derived auxin-inducible degradation system (AID) enables the degradation of target proteins tagged with the AID motif. This system has been recently employed in mammalian cells as well as in Caenorhabditis elegans and Drosophila. To test the utility of the AID approach in the nervous system, we used circadian locomotor rhythms as a model and applied the AID method to temporally and spatially degrade PERIOD (PER), a critical pacemaker protein in Drosophila. We found that the period locus can be efficiently tagged with the AID motif by CRISPR/Cas9-based genome editing without disrupting PER function. Moreover, we demonstrated that the AID system could be used to induce rapid and efficient protein degradation in the nervous system as shown by effects on circadian and sleep behaviors. Furthermore, the protein degradation by AID was rapidly reversible after auxin removal. Together, our results show that the AID system provides a powerful tool for behavior studies in Drosophila.
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http://dx.doi.org/10.1111/febs.14677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289745PMC
December 2018