Publications by authors named "Wendell B Jones"

2 Publications

  • Page 1 of 1

Capsulorhexis challenge with long anterior lens zonules.

Am J Ophthalmol Case Rep 2020 Sep 27;19:100756. Epub 2020 May 27.

Illinois Eye Institute, Department of Clinical Education, Illinois College of Optometry, 3241, S. Michigan Ave, Chicago, IL, USA.

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http://dx.doi.org/10.1016/j.ajoc.2020.100756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7276380PMC
September 2020

Translational profiling of hypocretin neurons identifies candidate molecules for sleep regulation.

Genes Dev 2013 Mar 21;27(5):565-78. Epub 2013 Feb 21.

Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA.

Hypocretin (orexin; Hcrt)-containing neurons of the hypothalamus are essential for the normal regulation of sleep and wake behaviors and have been implicated in feeding, anxiety, depression, and reward. The absence of these neurons causes narcolepsy in humans and model organisms. However, little is known about the molecular phenotype of these cells; previous attempts at comprehensive profiling had only limited sensitivity or were inaccurate. We generated a Hcrt translating ribosome affinity purification (bacTRAP) line for comprehensive translational profiling of all ribosome-bound transcripts in these neurons in vivo. From this profile, we identified >6000 transcripts detectably expressed above background and 188 transcripts that are highly enriched in these neurons, including all known markers of the cells. Blinded analysis of in situ hybridization databases suggests that ~60% of these are expressed in a Hcrt marker-like pattern. Fifteen of these were confirmed with double labeling and microscopy, including the transcription factor Lhx9. Ablation of this gene results in a >30% loss specifically of Hcrt neurons, without a general disruption of hypothalamic development. Polysomnography and activity monitoring revealed a profound hypersomnolence in these mice. These data provide an in-depth and accurate profile of Hcrt neuron gene expression and suggest that Lhx9 may be important for specification or survival of a subset of these cells.
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http://dx.doi.org/10.1101/gad.207654.112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605469PMC
March 2013