Publications by authors named "Wenbo Wei"

32 Publications

A microfluidic array device for single cell capture and intracellular Ca2+ response analysis induced by dynamic biochemical stimulus.

Biosci Rep 2021 Jul;41(7)

First Affiliated Hospital of Shenzhen University (Shenzhen Second People's Hospital), Shenzhen 518035, China.

A microfluidic array was constructed for trapping single cell and loading identical dynamic biochemical stimulation for gain a better understanding of Ca2+ signaling at single cell resolution in the present study. This microfluidic array consists of multiple radially aligned flow channels with equal intersection angles, which was designed by a combination of stagnation point flow and physical barrier. Numerical simulation results and trajectory analysis have shown the effectiveness of this single cell trapping device. Fluorescent experiment results demonstrated the effects of flow rate and frequency of dynamic stimulus on the profiles of biochemical concentration which exposed on captured cells. In this microarray, the captured single cells in each trapping channels were able to receive identical extracellular dynamic biochemical stimuli which being transmitted from the entrance in the middle of the microfluidic array. Besides, after loading dynamic Adenosine Triphosphate (ATP) stimulation on captured cells by this device, consistent average intracellular Ca2+ dynamics phase and cellular heterogeneity were observed in captured single K562 cells. Furthermore, this device is able to be used for investigating cellular respond on single cell resolution to temporally varying environments by modulating the stimulation signal in terms of concentration, pattern, and duration of exposure.
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http://dx.doi.org/10.1042/BSR20210719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319492PMC
July 2021

AZnGaSe (A = K, Rb, or Cs): Infrared Nonlinear Optical Materials with Simultaneous Large Second Harmonic Generation Responses and High Laser-Induced Damage Thresholds.

Inorg Chem 2021 Jul 17;60(13):10038-10046. Epub 2021 Jun 17.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.

Despite the fact that nonlinear optical (NLO) crystals such as AgGaS and AgGaSe have been widely used in the infrared (IR) range due to their large second harmonic generation (SHG) coefficients and wide range of IR transparency windows, the small laser-induced damage threshold (LIDT) remains a great issue hindering their high-power applications. Herein, three noncentrosymmetric (NCS) chalcogenides AZnGaSe (A = K, Rb, or Cs) are successfully obtained through an appropriate flux method after the extensive design and synthesis of the A/Zn/Ga/Q system. Single-crystal X-ray diffraction data demonstrate that they adopt trigonal space group 3 (No. 146) with three-dimensional diamond-like frameworks composed of [MSe] layers (M = Zn or Ga) stacking in the same direction and filled by charge-balancing A cations. Noticeably, they all exhibit strong powder SHG responses (2.8-3.7 × AgGaS) and amazing LIDTs (19.2-23.4 × AgGaS). In addition, theoretical calculations are performed to further determine the relationship between NCS structures and NLO properties. This work provides effective solutions for overcoming the trade-off between strong SHG and high LIDT in IR-NLO materials.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01359DOI Listing
July 2021

Long noncoding RNA promotes migration of breast cancer via FXR 1.

Cell Adh Migr 2021 12;15(1):140-151

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, China.

LncRNA plays a critical role in tumor progression. However, the role it executes in breast cancer is still unclear. Here, we report a newly discovered lncRNA, , which could be remarkably up-regulated in breast cancer cells and tissues. We found that the expression of was positively correlated with tumor size, lymph node metastasis and HER2. More interesting, overexpression of significantly increased cell migration, while specific knockdown led to the opposite. RNA pull-down and RNA immunoprecipitation assays demonstrated that could directly bind to FXR1 to promote tumor metastasis. and FXR1 were positively correlated. FXR1 was also significantly up-regulated in breast tumors. Taken together, we propose that regulates FXR1 to promote breast cancer metastasis.
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http://dx.doi.org/10.1080/19336918.2021.1921402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168597PMC
December 2021

Ordered Macroporous Superstructure of Nitrogen-Doped Nanoporous Carbon Implanted with Ultrafine Ru Nanoclusters for Efficient pH-Universal Hydrogen Evolution Reaction.

Adv Mater 2021 Mar 18;33(12):e2006965. Epub 2021 Feb 18.

AIST-Kyoto University Chemical Energy Materials Open Innovation Laboratory (ChEM-OIL), National Institute of Advanced Industrial Science and Technology (AIST), Sakyo-ku, Kyoto, 6068501, Japan.

The electrochemical hydrogen evolution reaction (HER) is an attractive technology for the mass production of hydrogen. Ru-based materials are promising electrocatalysts owing to the similar bonding strength with hydrogen but much lower cost than Pt catalysts. Herein, an ordered macroporous superstructure of N-doped nanoporous carbon anchored with the ultrafine Ru nanoclusters as electrocatalytic micro/nanoreactors is developed via the thermal pyrolysis of ordered macroporous single crystals of ZIF-8 accommodating Ru(III) ions. Benefiting from the highly interconnected reticular macro-nanospaces, this superstrucure affords unparalleled performance for pH-universal HER, with order of magnitude higher mass activity compared to the benchmark Pt/C. Notably, an exceptionally low overpotential of only 13 [email protected] mA cm is required for HER in alkaline solution, with a low Tafel slope of 40.41 mV dec and an ultrahigh turnover frequency value of 1.6 H s at 25 mV, greatly outperforming Pt/C. Furthermore, the hydrogen generation rates are almost twice those of Pt/C during practical overall alkaline water splitting. A solar-to-hydrogen system is also demonstrated to further promote the application. This research may open a new avenue for the development of advanced electrocatalytic micro/nanoreactors with controlled morphology and excellent performance for future energy applications.
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http://dx.doi.org/10.1002/adma.202006965DOI Listing
March 2021

Quaternary Chalcohalides CdSnSX (X = Cl or Br) with Neutral Layers: Syntheses, Structures, and Photocatalytic Properties.

Inorg Chem 2021 Mar 17;60(5):3431-3438. Epub 2021 Feb 17.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.

Inorganic chalcohalides are attracting a tremendous amount of attention because of their remarkable structural variety and desirable physical properties. Although great advances have been made in recent years, functional inorganic chalcohalides with two-dimensional neutral layers are still rare. Herein, two novel chalcohalides CdSnSX (X = Cl or Br) with high yields were obtained by reacting CdX with SnS using a traditional solid-state method at 823 K. Both of these chalcohalides adopt orthorhombic space group (No. 63) with the following structural values: = 4.014(4)-4.064(2) Å, = 12.996(2)-13.746(3) Å, = 9.471(2)-9.621(2) Å, = 494.1(8)-537.5(2) Å, and = 4. The prominent architectural feature is the unique two-dimensional [CdSnSX] neutral layer consisting of composite [CdX] and [SnS] sublattices that are connected alternately through the Cd-S-Sn bonds along the plane. The [CdX] sublattice consists of a single octahedral chain of Cd-centered [CdXS] groups sharing -X edges, while the [SnS] sublattice consists of a bend-shaped chain of unusual [SnSX] units sharing vertices of S atoms. Significantly, each CdSnSX form (X = Cl or Br) shows high visible-light-induced photocatalytic activity for rhodamine B degradation, which is ∼7.0 times higher than that of nitrogen-doped TiO (TiON) under the same experimental conditions. This discovery enriches the categories of inorganic chalcohalides and provides more choices of candidate materials for photocatalytic applications.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00010DOI Listing
March 2021

A liver-on-a-chip for hepatoprotective activity assessment.

Biomicrofluidics 2020 Nov 30;14(6):064107. Epub 2020 Nov 30.

College of Pharmaceutical Science, Soochow University, 215123 Suzhou, China.

Hepatoprotectant is critical for the treatment of liver disease. This study first reported the application of a liver chip in the hepatoprotective effect assessment. We first established a biomimetic sinusoid-on-a-chip by laminating four types of hepatic cell lines (HepG2, HUVEC, LX-2, and U937 cells) in a single microchannel with the help of laminar flow in the microchannel and some micro-fences. This chip was straightforward to fabricate and operate and was able to be long-term cultured. It also demonstrated better hepatic activity (cell viability, albumin synthesis, urea secretion, and cytochrome P450 enzyme activities) over the traditional planar cell culture model. Then, we loaded three hepatoprotectants (tiopronin, bifendatatum, and glycyrrhizinate) into the chip followed by the addition of acetaminophen as a toxin. We successfully observed the hepatoprotective effect of these hepatoprotectants in the chip, and we also found that bifendatatum predominantly reduced alanine transaminase secretion, tiopronin predominantly reduced lactate dehydrogenase secretion, and glycyrrhizinate predominantly reduced aspartate transaminase secretion, which revealed the different mechanisms of these hepatoprotectants and provided a clue for following molecular biological study of the protecting mechanism.
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http://dx.doi.org/10.1063/5.0024767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710384PMC
November 2020

Efficient Carbon Dioxide Electroreduction over Ultrathin Covalent Organic Framework Nanolayers with Isolated Cobalt Porphyrin Units.

ACS Appl Mater Interfaces 2020 Aug 13;12(34):37986-37992. Epub 2020 Aug 13.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter (FJIRSM), Chinese Academy of Sciences (CAS), Fuzhou 350002, China.

Electrochemical CO reduction represents a sustainable approach for the conversion of CO into valuable fuels and chemicals. Here, we fabricated a series of composite nanomaterials through template-oriented polymerization of covalent organic frameworks (COFs) with isolated cobalt porphyrin units on amino-functionalized carbon nanotubes for efficient electrocatalytic CO reduction reaction (CORR). Compared with pure COFs, the hybrid form of ultrathin COF nanolayers wrapped on the conductive scaffold leads to distended current density and stable Faradaic efficiency for CO-to-CO conversion over a wide potential range. Specifically, the catalytic performances of the system can be finely optimized by the modification of the reticular structure with different functional groups. Our work gives a new strategy for the preparation of highly active and selective electrocatalysts for CORR.
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http://dx.doi.org/10.1021/acsami.0c06537DOI Listing
August 2020

Quantifying the impact of optical surface guidance in the treatment of cancers of the head and neck.

J Appl Clin Med Phys 2020 Jun 6;21(6):73-82. Epub 2020 Apr 6.

Department of Radiation Oncology, The Permanente Medical Group, Santa Clara, CA, USA.

Surface guided radiation therapy (SGRT) is increasingly being adopted for use in radiation treatment delivery for Head and Neck (H&N) cancer patients. This study investigated the improvement of patient setup accuracy and reduction of setup time for SGRT compared to a conventional setup. A total of 60 H&N cancer patients were retrospectively included. Patients were categorized into three groups: oral cavity, oropharynx and nasopharynx/sinonasal sites with 20 patients in each group. They were further separated into two (2) subgroups, depending on whether they were set up with the aid of SGRT. The Align-RT™ system was used for SGRT in this work. Positioning was confirmed by daily kV-kV imaging in conjunction with weekly CBCT scans. Translational and rotational couch shifts along with patient setup times were recorded. Imaging setup time, which was defined as the elapsed time from the acquisition of the first image set to the end of the last image set, was recorded. Average translational shifts were larger in the non-SGRT group. Vertical shifts showed the most significant reduction in the SGRT group for both oropharynx and oral cavity groups. Pitch corrections were significantly higher in the SGRT group for oropharynx patients and higher pitch corrections were also observed in the SGRT groups of oral cavity and nasopharynx/sinonasal patients. The average setup time when SGRT guidance was employed was shorter for all three treatment sites although this did not reach statistical significance. The largest time reduction between the SGRT and non-SGRT groups was seen in the nasopharynx/sinonasal group. This study suggests that the use of SGRT decreases the magnitude of translational couch shifts during patient setup. However, the rotational corrections needed were generally higher with SGRT group. When SGRT was employed, a definite reduction in patient setup time was observed for nasopharynx/sinonasal and hypopharynx cancer patients.
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http://dx.doi.org/10.1002/acm2.12867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324691PMC
June 2020

Ligand-assisted capping growth of self-supporting ultrathin FeNi-LDH nanosheet arrays with atomically dispersed chromium atoms for efficient electrocatalytic water oxidation.

Nanoscale 2020 Mar;12(10):5817-5823

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter (FJIRSM), Chinese Academy of Sciences (CAS), Fuzhou 350002, China.

Self-supporting ultrathin FeNi-layered double hydroxide nanosheet arrays with atomically dispersed Cr atoms were firstly fabricated from stainless steel mesh by a facile ligand-assisted capping growth approach. Their unique nanostructure and a strong synergetic effect between the atomically dispersed Cr dopants and the active sites afford an exceptional OER activity.
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http://dx.doi.org/10.1039/c9nr10781aDOI Listing
March 2020

Engineered Liver-on-a-Chip Platform to Mimic Liver Functions and Its Biomedical Applications: A Review.

Micromachines (Basel) 2019 Oct 7;10(10). Epub 2019 Oct 7.

College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.

Hepatology and drug development for liver diseases require in vitro liver models. Typical models include 2D planar primary hepatocytes, hepatocyte spheroids, hepatocyte organoids, and liver-on-a-chip. Liver-on-a-chip has emerged as the mainstream model for drug development because it recapitulates the liver microenvironment and has good assay robustness such as reproducibility. Liver-on-a-chip with human primary cells can potentially correlate clinical testing. Liver-on-a-chip can not only predict drug hepatotoxicity and drug metabolism, but also connect other artificial organs on the chip for a human-on-a-chip, which can reflect the overall effect of a drug. Engineering an effective liver-on-a-chip device requires knowledge of multiple disciplines including chemistry, fluidic mechanics, cell biology, electrics, and optics. This review first introduces the physiological microenvironments in the liver, especially the cell composition and its specialized roles, and then summarizes the strategies to build a liver-on-a-chip via microfluidic technologies and its biomedical applications. In addition, the latest advancements of liver-on-a-chip technologies are discussed, which serve as a basis for further liver-on-a-chip research.
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http://dx.doi.org/10.3390/mi10100676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843249PMC
October 2019

[Effectiveness of internal fixation with headless compression hollow embedding screws for intraarticular fracture of elbow].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2019 Aug;33(8):986-990

Department of Orthopedics, Shaanxi People's Hospital, Xi'an Shaanxi, 710068, P.R.China.

Objective: To evaluate the effectiveness of internal fixation with headless compression hollow embedding screws in the treatment of intraarticular fracture of elbow.

Methods: Between March 2012 and September 2018, 12 patients with intraarticular fracture of elbow were treated with internal fixation with headless compression hollow embedding screws. There were 7 males and 5 females with an average age of 50.3 years (range, 22-65 years). Cause of injury included falling in 7 cases, falling from high places in 4 cases, and traffic accident in 1 case. Ten patients were distal humerus fractures which were classified as type 13-B3 in 8 cases and type 13-C3 in 2 cases according to the International Association of Internal Fixation Research (AO/ASIF). Two patients were radial head fractures which were classified as type Ⅲ according to the modified Mason classifications. The preoperative visual analogue scale (VAS) score was 8.25±0.83. The time from injury to operation was 3-5 days (mean, 3.7 days).

Results: All incisions healed by first intention. All 12 patients were followed up 6-15 months, with an average of 8.4 months. The results of X-ray films and CT examination showed that the fracture ends were anatomic reduction, and the fractures healed at 6-11 months after operation, with an average of 7.8 months. One patient had heterotopic ossification at 4 months after operation. The VAS scores were 5.17±0.79 at 2 weeks after operation and 0.50±0.50 at last follow-up. There were significant differences between the time points ( <0.05). At last follow-up, the Mayo elbow function score was 68-95, with an average of 83.9. The activity of elbow joint recovered.

Conclusion: The intraarticular fracture of elbow can be firmly fixed by the headless compression hollow embedding screw, which can allow the early functional training of the elbow joint, reduce the incidence of heterotopic ossification, and obtain good effectiveness.
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http://dx.doi.org/10.7507/1002-1892.201902058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8337900PMC
August 2019

MiR-19b-3p attenuates IL-1β induced extracellular matrix degradation and inflammatory injury in chondrocytes by targeting GRK6.

Mol Cell Biochem 2019 Sep 21;459(1-2):205-214. Epub 2019 Jun 21.

Department of Orthopaedics, Shaanxi Provincial People's Hospital, NO. 256 Youyi Xi Road, Xi'an, 710068, Shaanxi, China.

Osteoarthritis (OA) is characterized by degradation of articular cartilage. MiRNAs are involved in the regulation of chondrogenesis and OA. We aimed to investigate effects and mechanisms of miR-19b-3p in regulating chondrocytes viability, cartilage degradation and inflammatory response. Primary chondrocytes were isolated from cartilages in control subjects and patients with OA. Murine ATDC5 cells were pre-conditioned with IL-1β in vitro. Expressions and interaction of miR-19b-3p with G protein-coupled receptor kinase 6 (GRK6), and their effects on inflammation, chondrocytes viability and cartilage degradation were determined after miR-19b-3p mimic or GRK6 siRNA transfection. MiR-19b-3p was significantly decreased in OA chondrocytes and IL-1β-stimulated ATDC5 cells, in paralleled with the elevated type-II-collagen, aggrecan, MMP13 and GRK6 expression. MiR-19b-3p mimic dramatically increased the viability of chondrocytes and suppressed cell apoptosis. It also increased type-II-collagen, aggrecan expression and glycosaminoglycan (sGAG) content, and decreased the expression of MMP-1 and MMP-13 that controlled by IL-1β. Overexpression of miR-19b-3p inhibited the production of IL-6 and IL-8 in ATDC5 cells. However, the protective effects of miR-19b-3p mimic on IL-1β induced cell death; IL-8 production and sGAG decrease were greatly discounted by GRK6 lentiviral vectors. Luciferase reporter assay confirmed that GRK6 gene was a direct target ofmiR-19b-3p. GRK6 siRNA transfection antagonized the IL-1β-induced chondrocytes injury, extracellular matrix degradation and inflammatory response. MiR-19b-3p mimic and GRK6 siRNA showed comparable inhibitory effect on IL-1β-provoked NF-κB as reflected by the expression of p-p65. NF-κB translocation inhibition with PS1154 reversed the effects of IL-1β on IL-8 and sGAG. Collectively, miR-19b-3p attenuated OA by targeting GRK6-NF-κB pathway.
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http://dx.doi.org/10.1007/s11010-019-03563-2DOI Listing
September 2019

Oriented Gold Nanorod Arrays: Self-Assembly and Optoelectronic Applications.

Angew Chem Int Ed Engl 2019 Aug 25;58(35):11956-11966. Epub 2019 Jun 25.

Department of Chemical and Biological Engineering, The University of New Mexico, Albuquerque, NM, 87131, USA.

Self-assembly of anisotropic plasmonic nanomaterials into ordered superstructures has become popular in nanoscience because of their unique anisotropic optical and electronic properties. Gold nanorods (GNRs) are a well-defined functional building block for fabrication of these superstructures. They possess important anisotropic plasmonic characteristics that result from strong local electric field and are responsive to visible and near-IR light. There are recent examples of assembling the GNRs into ordered arrays or superstructures through processes such as solvent evaporation and interfacial assembly. In this Minireview, recent progress in the development of the self-assembled GNR arrays is described, with focus on the formation of oriented GNR arrays on substrates. Key driving forces are discussed, and different strategies and self-assembly processes of forming oriented GNR arrays are presented. The applications of the oriented GNR arrays in optoelectronic devices are also overviewed, especially surface enhanced Raman scattering (SERS).
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http://dx.doi.org/10.1002/anie.201902620DOI Listing
August 2019

Surfactant-Assisted Cooperative Self-Assembly of Nanoparticles into Active Nanostructures.

iScience 2019 Jan 27;11:272-293. Epub 2018 Dec 27.

Department of Chemical and Biological Engineering, The University of New Mexico, Albuquerque, NM 87131, USA; Advanced Materials Laboratory, Sandia National Laboratories, Albuquerque, NM 87106, USA; Center for Integrated Nanotechnologies, Sandia National Laboratories, Albuquerque, NM 87185, USA. Electronic address:

Nanoparticles (NPs) of controlled size, shape, and composition are important building blocks for the next generation of devices. There are numerous recent examples of organizing uniformly sized NPs into ordered arrays or superstructures in processes such as solvent evaporation, heterogeneous solution assembly, Langmuir-Blodgett receptor-ligand interactions, and layer-by-layer assembly. This review summarizes recent progress in the development of surfactant-assisted cooperative self-assembly method using amphiphilic surfactants and NPs to synthesize new classes of highly ordered active nanostructures. Driven by cooperative interparticle interactions, surfactant-assisted NP nucleation and growth results in optically and electrically active nanomaterials with hierarchical structure and function. How the approach works with nanoscale materials of different dimensions into active nanostructures is discussed in details. Some applications of these self-assembled nanostructures in the areas of nanoelectronics, photocatalysis, and biomedicine are highlighted. Finally, we conclude with the current research progress and perspectives on the challenges and some future directions.
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http://dx.doi.org/10.1016/j.isci.2018.12.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327881PMC
January 2019

Iodine catalyzed reduction of quinolines under mild reaction conditions.

Chem Commun (Camb) 2018 Aug 18;54(62):8622-8625. Epub 2018 Jul 18.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P. R. China.

A reduction of quinolines to synthetically versatile tetrahydroquinoline molecules with I and HBpin is described. In the presence of iodine (20 mol%) as a catalyst, reduction of quinolines and other N-heteroarenes proceeded readily with hydroboranes as the reducing reagents. The broad functional-group tolerance, good yields and mild reaction conditions imply high practical utility.
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http://dx.doi.org/10.1039/c8cc04262dDOI Listing
August 2018

Fabrication of Large-Area Arrays of Vertically Aligned Gold Nanorods.

Nano Lett 2018 07 28;18(7):4467-4472. Epub 2018 Jun 28.

Department of Chemical and Biological Engineering , The University of New Mexico , Albuquerque , New Mexico 87131 , United States.

Anisotropic nanoparticles, such as nanorods and nanoprisms, enable packing of complex nanoparticle structures with different symmetry and assembly orientation, which result in unique functions. Despite previous extensive efforts, formation of large areas of oriented or aligned nanoparticle structures still remains a great challenge. Here, we report fabrication of large-area arrays of vertically aligned gold nanorods (GNR) through a controlled evaporation deposition process. We began with a homogeneous suspension of GNR and surfactants prepared in water. During drop casting on silicon substrates, evaporation of water progressively enriched the concentrations of the GNR suspension, which induces the balance between electrostatic interactions and entropically driven depletion attraction in the evaporating solution to produce large-area arrays of self-assembled GNR on the substrates. Electron microscopy characterizations revealed the formation of layers of vertically aligned GNR arrays that consisted of hexagonally close-packed GNR in each layer. Benefiting from the close-packed GNR arrays and their smooth topography, the GNR arrays exhibited a surface-enhanced Raman scattering (SERS) signal for molecular detection at a concentration as low as 10 M. Because of the uniformity in large area, the GNR arrays exhibited exceptional detecting reproducibility and operability. This method is scalable and cost-effective and could lead to diverse packing structures and functions by variation of guest nanoparticles in the suspensions.
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http://dx.doi.org/10.1021/acs.nanolett.8b01584DOI Listing
July 2018

Comparison of intravenous and topical tranexamic acid in total knee arthroplasty.

BMC Musculoskelet Disord 2018 Jun 13;19(1):191. Epub 2018 Jun 13.

Department of Pain, YangLing Demonstration Zone Hospital, No.15 Kangle street, Yang ling, Xi'an, 712100, China.

Background: To investigate the clinical effectiveness of intravenous (IV) and topical tranexamic acid (TXA) in patients undergoing total knee arthroplasty (TKA) by comparing safety, efficacy and patient-reported outcomes.

Methods: In this prospective single-blind clinical trial, 64 patients were randomized into two groups (n = 32 each). The Intravenous Group was administered TXA 10 mg/kg IV (Reyong, Shandong, China) 10 min prior to tourniquet deflation. In the Topical Group, 1.0 g TXA diluted in 50 ml of normal saline was injected into the surgical site, which was bathed in the solution for at least 5 min prior to tourniquet deflation. Outcomes included changes in hemoglobin levels, intra-operative, post-operative, and total blood loss, number of transfusions and number of transfused units, patient-reported postoperative Visual Analog Scale (VAS) score for knee pain, and complications.

Results: There were no significant differences in intra-operative blood loss, post-operative blood loss, total blood loss, or post-operative decrease in hemoglobin in the Intravenous Group versus the Topical Group. The number of transfused red blood cell units was significantly greater and-post-operative VAS score was significantly lower in the Intravenous Group. There were no differences in post-operative thromboembolic complications between groups.

Conclusions: Topical TXA is not inferior to IV administration in reducing perioperative blood loss in primary TKA. However, the influence of injection volume of locally applied TXA on post-operative knee pain warrants further investigation.

Trial Registration: Clinical ethics committee of Shaanxi People's Hospital (2009), No.125. ( ChiCTR 1,800,015,793 ) registered on 20/04/2018.
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http://dx.doi.org/10.1186/s12891-018-2122-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000941PMC
June 2018

Three MOF-Templated Carbon Nanocomposites for Potential Platforms of Enzyme Immobilization with Improved Electrochemical Performance.

ACS Appl Mater Interfaces 2018 May 17;10(17):14665-14672. Epub 2018 Apr 17.

Institute of Environmental and Food Safety , Wuhan Academy of Agricultural Science and Technology , Wuhan 430207 , People's Republic of China.

An efficient and facile metal-organic framework (MOF)-template strategy for preparing carbon nanocomposites has been developed. First of all, a series of metal ions, including Fe, Zr, and La, were respectively connected with 2-aminoterephthalate (HATA) to form three metal-organic frameworks (MOFs) and then three novel MOF-derived materials were obtained by annealing them at 550 °C under N atmosphere. The morphologies and microstructure results showed that they still retained the original structure of MOFs and formed carbon-supported metal oxide hybrid nanomaterials. Interestingly, it was found that La-MOF-NH and its derived materials were first reported, which had wool-ball-like structure formed by many streaky-shaped particles intertwining each other. Furthermore, these MOF-derived materials were all successfully used as effective immobilization matrixes of acetylcholinesterase (AChE) to construct biosensors for the detection of methyl parathion. Especially, [La-MOF-NH] with wool-ball-like structure not only provided more active sites of multicontents to increase AChE immobilization amount but also facilitated the accessibility of electron transfer and shorten their diffusion length on the surface of electrode. Under optimal conditions, the biosensor based on [La-MOF-NH] displayed the widest linear range of 1.0 × 10-5.0 × 10 g mL and the lowest detection limit of 5.8 × 10 g mL in three biosensors. This study illustrates the feasibility and the potential of a series of MOF-derived materials for biosensors with improved electrochemical performance.
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http://dx.doi.org/10.1021/acsami.8b00702DOI Listing
May 2018

A Phase I clinical trial of the knee to assess the correlation of gagCEST MRI, delayed gadolinium-enhanced MRI of cartilage and T2 mapping.

Eur J Radiol 2017 May 6;90:220-224. Epub 2017 Mar 6.

Wright Center of Innovation in Biomedical Imaging and Department of Radiology, The Ohio State University, Columbus, OH, United States. Electronic address:

Purpose: Osteoarthritis (OA) is associated with the loss of glycosaminoglycan (GAG) during disease progression, which can be detected by glycosaminoglycan chemical exchange-dependent saturation transfer (gagCEST) MRI. Delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) is considered one of the standard methods for GAG quantification in vivo. This Phase I study assessed the correlation between gagCEST MRI and dGEMRIC in determining cartilage GAG concentration. Standard T2 mapping was used as a comparator with the two other methods.

Materials And Methods: Eight athletic volunteers with no known knee diseases were recruited in this study. The sagittal images of both knees in each volunteer were obtained by a 3T MRI system. GAG concentration was calculated based on fixed charge density (FCD) within articular cartilage as calculated by T1 values obtained from dGEMRIC sequences. Magnetization transfer ratio asymmetry (MTR) of the CEST spectrum at 1ppm was determined with gagCEST MRI. T2 values were calculated using a multi-echo turbo spin echo (TSE) sequence. The Pearson correlations among MTR were calculated from gagCEST analysis.

Results: There was moderate correlation (correlation coefficient r=0.55) between dGEMRIC and gagCEST MRI results. T2 had a low correlation (r=-0.30) with gagCEST and no correlation with dGEMRIC (r=0.003). Both gagCEST and dGEMRIC were able to distinguish between high GAG concentration cartilage compartments (higher than 210mM) and low GAG cartilage compartments (lower than 210mM).

Conclusion: dGEMRIC was shown to be a more accurate and sensitive clinical imaging tool in evaluating cartilage GAG levels in vivo. While GagCEST showed less sensitivity to GAG concentration variations than dGEMRIC, further improvements may yet enable gagCEST to be a clinically robust methodology.
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http://dx.doi.org/10.1016/j.ejrad.2017.02.030DOI Listing
May 2017

ACL graft metabolic activity assessed by FDG PET-MRI.

Knee 2017 Aug 27;24(4):792-797. Epub 2017 May 27.

Wright Center of Innovation in Biomedical Imaging, The Ohio State University, Columbus, OH, United States; Department of Radiology, The Ohio State University, Columbus, OH, United States.

Background: To demonstrate the use of Fluorodeoxyglucose positron emission tomography (PET) and magnetic resonance imaging (MRI) in combination (FDG-PET) to assess the metabolic activity of ACL graft tissue and evaluate the utility of this technique for ligament imaging.

Methods: Twenty-one knees with intact ACL grafts in 19 patients at multiple time points following ACL reconstruction were recruited to participate. PET-MRI imaging was performed using a custom device to place knees in the same position for both studies. Images were co-registered for quantification of FDG-PET standardized uptake value (SUV) for the proximal, middle, and distal ACL was quantified. Signal in extra-articular muscle tissue in the index knee was also recorded as a control. Signal from each location was compared based on how far post-operative each knee was from ACL reconstruction (<6months, six to 12months, 12-24months, or >24months).

Results: Significant differences in FDG PET SUV between the four time points were observed in the proximal (p=0.02), middle (p=0.004), and distal (p=0.007) portions of the ACL graft. The greater than 24months group was noted to be different from other groups in each case. No difference in PET FDG SUV was noted in the extra-articular muscle in the index knee in each time group (p=0.61).

Conclusions: Metabolic activity was noted to be significantly lower in grafts imaged greater than two years post-reconstruction relative to those grafts that had been in place for shorter periods of time.
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http://dx.doi.org/10.1016/j.knee.2017.04.008DOI Listing
August 2017

Dynamic Contrast-Enhanced Magnetic Resonance Imaging of Ocular Melanoma as a Tool to Predict Metastatic Potential.

J Comput Assist Tomogr 2017 Sep/Oct;41(5):823-827

From the *Wright Center of Innovation in Biomedical Imaging and Department of Radiology, The Ohio State University, Columbus, OH; †Department of Physics and Astronomy, Louisiana State University, Baton Rouge, LA; ‡Department of Diagnostic, Interventional and Pediatric Radiology, Inselspital, University of Bern, Bern, Switzerland; §Department of Ophthalmology and Visual Science, and ∥Center for Biostatistics, Department of Biomedical Informatics, The Ohio State University, Columbus, OH; and ¶Department of Ophthalmology and Vision Science, The University of Arizona Medical Center, Tucson, AZ.

Purpose: This study explores the capability of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) to differentiate tumor characteristics of metastatic and nonmetastatic choroidal melanoma as a potential tool for patient management.

Materials And Methods: A total of 13 patients (69 ± 9 years) with choroidal melanoma were imaged using DCE-MRI on a 3-T MRI system with a 16-channel head coil. The Tofts 2-compartment model was chosen for quantification, and parameters K (the transfer constant from the blood plasma to the extracellular space) and Kep (the transfer constant from the extracellular space to the blood plasma) were calculated and compared. Metastasis was excluded by subsequent clinical work-up or confirmed by histology after targeted biopsy.

Results: Six patients were diagnosed with metastatic melanoma and 7 without. All orbital tumors were at least larger than 2 mm. A significant difference was identified in K between patients with (0.73 ± 0.18/min) and without (1.00 ± 0.21/min) metastatic melanoma (P = 0.03), whereas the difference was not significantly shown in Kep (2.58 ± 1.54/min of metastatic patients vs 2.98 ± 1.83/min of nonmetastatic patients, P = 0.67).

Conclusions: Dynamic contrast-enhanced magnetic resonance imaging has the potential to differentiate orbital melanomas with metastatic and nonmetastatic spread. Thus, DCE-MRI has the potential to be an in vivo imaging technique to predict early which patients are prone to metastatic disease.
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http://dx.doi.org/10.1097/RCT.0000000000000598DOI Listing
October 2017

Assessing the effect of football play on knee articular cartilage using delayed gadolinium-enhanced MRI of cartilage (dGEMRIC).

Magn Reson Imaging 2017 06 12;39:149-156. Epub 2017 Jan 12.

Wright Center of Innovation in Biomedical Imaging, Department of Radiology, The Ohio State University, Columbus, OH, United States. Electronic address:

The prevalence of cartilage lesions is much higher in football athletes than in the general population. Delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) has been shown to quantify regional variations of glycosaminoglycan (GAG) concentrations which is an indicator of early cartilage degeneration. The goal of this study is to determine whether dGEMRIC can be used to assess the influence in cartilage GAG concentration due to college level football play. Thirteen collegiate football players with one to four years of collegiate football play experience were recruited and both knee joints were scanned using a dedicated 8-channel phased array knee coil on a 3T MRI system. The contrast concentrations within cartilage were calculated based on the T values from dGEMRIC scans. No substantial differences were found in the contrast concentrations between the pre- and post-season across all the cartilage compartments. One year collegiate football players presented an average contrast concentration at the pre-season of 0.116±0.011mM and post-season of 0.116±0.011mM. In players with multiple years of football play, contrast uptake was elevated to 0.141±0.012mM at the pre-season and 0.139±0.012mM at the post-season. The pre-season 0.023±0.016mM and post-season 0.025±0.016mM increase in contrast concentration within the group with multiple years of experience presented with a >20% increase in contrast uptake. This may indicate the gradual, cumulative damage of football play to the articular cartilage over years, even though the effect may not be noticeable after a season of play. Playing collegiate football for a longer period of time may lead to cartilage microstructural alterations, which may be linked to early knee cartilage degeneration.
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http://dx.doi.org/10.1016/j.mri.2017.01.008DOI Listing
June 2017

Synthesis of reticulated hollow spheres structure NiCoS and its application in organophosphate pesticides biosensor.

Biosens Bioelectron 2017 Jun 24;92:563-569. Epub 2016 Oct 24.

School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.

Electrode materials play a key role in the development of electrochemical sensors, particularly enzyme-based biosensors. Here, a novel NiCoS with reticulated hollow spheres assembled from rod-like structures was prepared by a one-pot solvothermal method and its formation mechanism was discussed. Moreover, comparison of NiCoS materials from different experiment conditions as biosensors was investigated by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV), and the best one that was reticulated hollow spheres assembled from rod-like structures NiCoS has been successfully employed as a matrix of AChE immobilization for the special structure, superior conductivity and rich reaction active sites. When using common two kinds of organophosphate pesticides (OPs) as model analyte, the biosensors demonstrated a wide linear range of 1.0×10-1.0×10gmL with the detection limit of 4.2×10gmL for methyl parathion, and 1.0×10-1.0×10gmL with the detection limit of 3.5×10gmL for paraoxon, respectively. The proposed biosensors exhibited many advantages such as acceptable stability and low cost, providing a promising tool for analysis of OPs.
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http://dx.doi.org/10.1016/j.bios.2016.10.059DOI Listing
June 2017

Exploiting multi-function Metal-Organic Framework nanocomposite [email protected] as highly efficient immobilization matrixes for sensitive electrochemical biosensing.

Anal Chim Acta 2016 Aug 2;934:203-11. Epub 2016 Jun 2.

School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.

A novel multi-function Metal-Organic Framework composite [email protected] (zinc thiosalicylate, Zn(C7H4O2S), Zn-TSA) was synthesized as highly efficient immobilization matrixes of myoglobin (Mb)/glucose oxidase (GOx) for electrochemical biosensing. The electrochemical biosensors based on [email protected] composite and ionic liquid (IL) modified carbon paste electrode (CPE) were fabricated successfully. Furthermore, the properties of the sensors were discussed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and amperometric current-time curve, respectively. The results showed the proposed biosensors had wide linear response to hydrogen peroxide (H2O2) in the range of 0.3-20,000 μM, to nitrite (NO2(-)) for 1.3 μM-1660 μM and 2262 μM-1,33,000 μM, to glucose for 2.0-1022 μM, with a low detection limit of 0.08 μM for H2O2, 0.5 μM for NO2(-), 0.8 μM for glucose. The values of the apparent heterogeneous electron transfer rate constant (ks) for Mb and GOx were estimated as 2.05 s(-1) and 2.45 s(-1), respectively. Thus, [email protected] was a kind of ideal material as highly efficient immobilization matrixes for sensitive electrochemical biosensing. In addition, this work indicated that MOF nanocomposite had a great potential for constructing wide range of sensing interface.
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http://dx.doi.org/10.1016/j.aca.2016.05.040DOI Listing
August 2016

[PART-KESSLER TECHNIQUE WITH SUTURE ANCHOR IN REPAIR OF SPONTANEOUS Achilles TENDON RUPTURE].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2016 Feb;30(2):165-8

Objective: To summarize the application and experience of repairing spontaneous Achilles tendon rupture by part-Kessler technique with suture anchor.

Methods: Between January 2011 and December 2013, 31 patients with spontaneous Achilles tendon rupture were treated by part-Kessler technique with suture anchor. Of 31 cases, 23 were male and 8 were female, aged 16-53 years (mean, 38 years). The left side was involved in 15 cases and the right side in 16 cases. The causes of injury included sudden heel pain and walking weakness during sports in 22 cases; no surefooted down-stairs, slip, and carrying heavy loads in 9 cases. The distance from broken site to the calcaneus adhension of Achilles tendon was 3-6 cm (mean, 4.2 cm). The time from injury to operation was 7 hours to 4 days (mean, 36.8 hours).

Results: All incisions healed by first intention without nerve injury or adhering with skin. The patients were followed up 6-24 months (mean, 15 months). All patients could complete 25 times heel raising without difficulty at 6 months after operation. No Achilles tendon rupture occurred again during follow-up. At 6 months after operation, the range of motion of the ankle joint in dorsiflexion and plantar flexion showed no significant difference between normal and affected sides (t=0.648, P=0.525; t=0.524, P=0.605). The circumference of the affected leg was significantly smaller than that of normal leg at 6 months after operation (t=2.074, P=0.041), but no significant difference was found between affected and normal sides at 12 months after operation (t=0.905, P=0.426). The American Orthopedic Foot and Ankle Society (AOFAS) scores at 6, 12, 18, and 24 months after operation were significantly higher than preoperative score (P<0.05); the score at 6 months after operation was significantly lower than that at other time points (P<0.05), but no significant difference was shown between the other time points (P>0.05).

Conclusion: Repairing spontaneous Achilles tendon rupture by part-Kessler technique with suture anchor can supply strong strain and decrease the shear forces of suture. So part-Kessler technique with suture anchor is successful in repairing spontaneous Achilles tendon rupture.
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February 2016

Simple Spinning of Heterogeneous Hollow Microfibers on Chip.

Adv Mater 2016 Aug 17;28(31):6649-55. Epub 2016 May 17.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian, 116023, China.

A novel and simple chip-based microfluidic strategy is proposed for continuously controlled spinning of desirable hollow microfibers. These fabricated fiber-shaped materials exhibit extraordinary morphological and structural complexity, as well as a heterogeneous composition. The resulting specific hollow microfibers have potential applications in numerous chemical and biomedical fields.
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http://dx.doi.org/10.1002/adma.201601504DOI Listing
August 2016

Modeling type 2 diabetes-like hyperglycemia in C. elegans on a microdevice.

Integr Biol (Camb) 2016 Jan 14;8(1):30-8. Epub 2015 Dec 14.

Laboratory of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.

Caenorhabditis elegans (C. elegans) has been widely used as a model organism for biomedical research due to its sufficient homology with mammals at the molecular and genomic levels. In this work, we describe a microfluidic assay to model type 2 diabetes-like hyperglycemia in C. elegans to examine several aspects of this disease on a microdevice. The microdevice is characterized by the integration of long-term worm culture, worm immobilization, and precise chemical stimuli in a single device, thus enabling the multi-parameter analysis of individual worms at a single-animal resolution. With this device, the lifespan, oxidative stress responses, and lipid metabolism of individual worms in response to different glucose concentrations were characterized. It was found that the mean lifespan of worms was significantly reduced by as much as 29.0% and 30.8% in worms that were subjected to 100 mM and 200 mM glucose, respectively. The expression of oxidative stress protein gst-4 was increased, and the expression of hsp-70 (heat shock protein) and skn-1 (redox sensitive transcription factor) genes was down-regulated in worms treated with a high level of glucose. Moreover, fat storage was markedly increased in the bodies of VS29 worms (vha-6p::GFP::dgat-2) that were exposed to the high-glucose condition. The established approach is not only suitable for further elucidation of the mechanism of metabolic disorders involved in diabetes and its complications, but also facilitates the evaluation of anti-diabetic drugs in a high-throughput manner.
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http://dx.doi.org/10.1039/c5ib00243eDOI Listing
January 2016

Morphology-controlled self-assembly and synthesis of photocatalytic nanocrystals.

Nano Lett 2014 Dec 6;14(12):7175-9. Epub 2014 Nov 6.

Key Laboratory for Special Functional Materials of the Ministry of Education, Henan University , Kaifeng 475004, People's Repubic of China.

Abilities to control the size and shape of nanocrystals in order to tune functional properties are an important grand challenge. Here we report a surfactant self-assembly induced micelle encapsulation method to fabricate porphyrin nanocrystals using the optically active precursor zinc porphyrin (ZnTPP). Through confined noncovalent interactions of ZnTPP within surfactant micelles, nanocrystals with a series of morphologies including nanodisk, tetragonal rod, and hexagonal rod, as well as amorphous spherical particle are synthesized with controlled size and dimension. A phase diagram that describes morphology control is achieved via kinetically controlled nucleation and growth. Because of the spatial ordering of ZnTPP, the hierarchical nanocrystals exhibit both collective optical properties resulted from coupling of molecular ZnTPP and shape dependent photocatalytic activities in photo degradation of methyl orange pollutants. This simple ability to exert rational control over dimension and morphology provides new opportunities for practical applications in photocatalysis, sensing, and nanoelectronics.
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http://dx.doi.org/10.1021/nl503761yDOI Listing
December 2014

Chemical exchange saturation transfer MR imaging of articular cartilage glycosaminoglycans at 3 T: Accuracy of B0 Field Inhomogeneity corrections with gradient echo method.

Magn Reson Imaging 2014 Jan 9;32(1):41-7. Epub 2013 Oct 9.

Wright Center of Innovation in Biomedical Imaging and Department of Radiology, The Ohio State University, Columbus, OH, United States.

Glycosaminoglycan Chemical Exchange Saturation Transfer (gagCEST) is an important molecular MRI methodology developed to assess changes in cartilage GAG concentrations. The correction for B0 field inhomogeneity is technically crucial in gagCEST imaging. This study evaluates the accuracy of the B0 estimation determined by the dual gradient echo method and the effect on gagCEST measurements. The results were compared with those from the commonly used z-spectrum method. Eleven knee patients and three healthy volunteers were scanned. Dual gradient echo B0 maps with different ∆TE values (1, 2, 4, 8, and 10 ms) were acquired. The asymmetry of the magnetization transfer ratio at 1 ppm offset referred to the bulk water frequency, MTRasym(1 ppm), was used to quantify cartilage GAG levels. The B0 shifts for all knee patients using the z-spectrum and dual gradient echo methods are strongly correlated for all ∆TE values used (r = 0.997 to 0.786, corresponding to ∆TE = 10 to 1 ms). The corrected MTRasym(1 ppm) values using the z-spectrum method (1.34% ± 0.74%) highly agree only with those using the dual gradient echo methods with ∆TE = 10 ms (1.72% ± 0.80%; r = 0.924) and 8 ms (1.50% ± 0.82%; r = 0.712). The dual gradient echo method with longer ∆TE values (more than 8 ms) has an excellent correlation with the z-spectrum method for gagCEST imaging at 3T.
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http://dx.doi.org/10.1016/j.mri.2013.07.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5743012PMC
January 2014

Strongly coupled nanorod vertical arrays for plasmonic sensing.

Adv Mater 2013 Jul 31;25(28):3863-8. Epub 2013 May 31.

National Center for Nanoscience and Technology, Beijing 100190, PR China.

Due to their unique optical properties and facile processability, nanorods of noble metals are promising for highly effective nanoscale optical devices. Specifically, the local electric field enhancement brought about by plasmon coupling between nanorods in an array configuration shows great potential for optical sensing. Recent results demonstrate that vertical arrays of noble metal nanorods, used as substrates for surface enhanced Raman scattering, can achieve the sensitivity levels required for presymptomatic detection. Meanwhile, advancements in controlled fabrication methods can provide nanorod arrays with well-defined structures and properties, which lays the foundation for highly sensitive and reliable sensing. This research news focuses on this rapidly developing field by introducing the mechanisms, characteristics, and preparation methods of nanorod arrays used in plasmonic sensing, along with a perspective for future development and technical requirements.
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http://dx.doi.org/10.1002/adma.201301181DOI Listing
July 2013
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