Publications by authors named "Wen-Kai Wang"

9 Publications

  • Page 1 of 1

The antidepressant-like effects of Danggui Buxue Decoction in GK rats by activating CREB/BDNF/TrkB signaling pathway.

Phytomedicine 2021 May 21;89:153600. Epub 2021 May 21.

College of Traditional Chinese Medicine•College of Intergrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, 138 Xianlin Rd, Nanjing, China. Electronic address:

Background: High rates of co-morbidity have been reported in patients with diabetes mellitus with depression (DD). Danggui Buxue Decoction (DBD), a Traditional Chinese Medicine formula composed of Angelica and Astragalus, has been historically used for the treatment of diabetes.

Purpose: This study aimed to investigated whether DBD and its main active component, ferulic acid (FA) from Angelica, could ameliorate depression-like behavior in DD and the underlying mechanisms.

Methods: Goto-Kakizaki (GK) rats were administered DBD (4 or 8 g/kg) by oral gavage during a 4-week period of chronic unpredictable mild stress. After 4 weeks, blood glucose, glycated serum protein, serum insulin, oral glucose tolerance and depression-like behavior were examined, along with brain-derived neurotrophic factor (BDNF)-related signaling pathway proteins and the ultrastructure of hippocampal tissues. UPLC-QTOF-MS was adopted to detect the absorption of FA in the serum and hippocampus. Rat primary hippocampal cells were cultured in a DD model. Protein and mRNA levels of genes involved in BDNF-related signaling and neuroplasticity were analyzed.

Results: DBD effectively improved glucose tolerance in DD rats and relieved depression-like behavior. Upregulation of cAMP response element binding protein (CREB), BDNF, and tropomyosin receptor kinase B (TrkB) and improvement of the hippocampal neuron ultrastructure supported the antidepressant-Like effects of DBD on the hippocampal neurons. In addition, DBD enhanced the protein and mRNA levels of components of the CREB/BDNF/TrkB pathway in rat primary hippocampal cells induced by elevated glycemia and cortisol. Interestingly, FA, the main component of DBD absorbed in the blood and hippocampus, showed similar effects as DBD on primary hippocampal cells.

Conclusion: This study suggests that the TCM formula DBD effectively serves as a potential therapeutic agent for prevention of DD through regulatory effects on the CREB/BDNF/TrkB pathway to protect and remodel hippocampal neurons. Moreover, FA contributes significantly to the treatment effects of DBD.
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http://dx.doi.org/10.1016/j.phymed.2021.153600DOI Listing
May 2021

A SEM study of antennal sensilla in Maladera orientalis Motschulsky (Coleoptera: Scarabaeidae: Melolonthinae).

Micron 2019 04 10;119:17-23. Epub 2019 Jan 10.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

The external morphology of antenna and fine structure of its sensilla of Maladera orientalis were studied using scanning electron microscopy. The antenna consists of scape, pedicel, funicle and a club composed of three lamellae. Funicle has five, sometimes, four segments. Böhm bristles, three subtypes of sensilla chaetica, one subtype of sensilla trichodea, one subtype of sensilla coeloconica, four subtypes of sensilla basiconica, and one subtype of sensilla placodea were described. No sexual differences were found in number, type and distribution of sensilla on all antennomeres. The most notable feature of sensilla on antennae of M. orientalis is the presence of long and narrow strip-like sensilla placodea on both sides of all club lamellae. These porous sensilla placodea are slightly depressed below the lamella surface and tend to lie in parallel with the lamella axe. They are similar to sensilla placodea found in hymenopteran species but very different from those round plate-like sensilla placodea occurring on the lamellae of scarab beetles.
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http://dx.doi.org/10.1016/j.micron.2019.01.004DOI Listing
April 2019

[Thermodynamic analysis of water adsorption and desorption process of Chinese herbal decoction pieces].

Zhongguo Zhong Yao Za Zhi 2016 Sep;41(18):3490-3495

Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.

Based on the basic theory of thermodynamics, the thermodynamic parameters and related equations in the process of water adsorption and desorption of Chinese herbal decoction pieces were established, and their water absorption and desorption characteristics were analyzed. The physical significance of the thermodynamic parameters, such as differential adsorption enthalpy, differential adsorption entropy, integral adsorption enthalpy, integral adsorption entropy and the free energy of adsorption, were discussed in this paper to provide theoretical basis for the research on the water adsorption and desorption mechanism, optimum drying process parameters, storage conditions and packaging methods of Chinese herbal decoction pieces.
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http://dx.doi.org/10.4268/cjcmm20161828DOI Listing
September 2016

[Isothermal adsorption, desorptionand thermodynamic properties of Scutellaria baicalensis pieces].

Zhongguo Zhong Yao Za Zhi 2016 Mar;41(5):830-837

Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.

To study the characteristics of adsorption and desorption of Scutellaria baicalensis pieces. On the basic thermodynamic theory, thestatic method was adopted to obtain S. baicalensis pieces' isothermal adsorption and desorption data at 25, 35, 45 ℃, with the water activity between 0.10 and 0.85. Eight moisture models were selected to fit the data and then evaluated to determine the thermodynamic properties of S. baicalensis pieces. The results show that, among the eight adsorption models, Peleg fit the best, in which absolutely-safe and the relatively-safe moisture contents of S. baicalensis pieces were around 9.22% and 13.51% respectively; the net equivalent heat adsorption and desorption and the differential entropy of S. baicalensis pieces were closely related to moisture content, and decrease within crease of water content; when drying the pieces, 12.0% of moisture content can be taken as the drying end; both of adsorption and desorption processes were driven by enthalpy and non-spontaneous. The study on S. baicalensis pieces' isothermal adsorption and desorption rules can help advance studies on adsorption and desorption rules of Chinese herbal pieces, and play a good guiding role in optimizing storage conditions and drying process of Chinese herbal pieces.
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http://dx.doi.org/10.4268/cjcmm20160512DOI Listing
March 2016

Distal-less regulates eyespot patterns and melanization in Bicyclus butterflies.

J Exp Zool B Mol Dev Evol 2013 Jul 30;320(5):321-31. Epub 2013 Apr 30.

Department of Biological Sciences, University at Buffalo, Buffalo, New York, USA.

Butterfly eyespots represent novel complex traits that display substantial diversity in number and size within and across species. Correlative gene expression studies have implicated a large suite of transcription factors, including Distal-less (Dll), Engrailed (En), and Spalt (Sal), in eyespot development in butterflies, but direct evidence testing the function of any of these proteins is still missing. Here we show that the characteristic two-eyespot pattern of wildtype Bicyclus anynana forewings is correlated with dynamic progression of Dll, En, and Sal expression in larval wings from four spots to two spots, whereas no such decline in gene expression ensues in a four-eyespot mutant. We then conduct transgenic experiments testing whether over-expression of any of these genes in a wild-type genetic background is sufficient to induce eyespot differentiation in these pre-patterned wing compartments. We also produce a Dll-RNAi transgenic line to test how Dll down-regulation affects eyespot development. Finally we test how ectopic expression of these genes during the pupal stages of development alters adults color patters. We show that over-expressing Dll in larvae is sufficient to induce the differentiation of additional eyespots and increase the size of eyespots, whereas down-regulating Dll leads to a decrease in eyespot size. Furthermore, ectopic expression of Dll in the early pupal wing led to the appearance of ectopic patches of black scales. We conclude that Dll is a positive regulator of focal differentiation and eyespot signaling and that this gene is also a possible selector gene for scale melanization in butterflies.
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http://dx.doi.org/10.1002/jez.b.22503DOI Listing
July 2013

A review of the genus Sinodorcadion Gressitt, 1939 with description of three new species from China (Coleoptera: Cerambycidae: Lamiinae).

Zootaxa 2013 ;3709:581-90

College of Life Sciences, Hebei University, Baoding, Hebei, 071002, P. R. China.

The genus Sinodorcadion Gressitt, 1939 is reviewed. Three new species, S. jiangi sp. nov., S. magnispinicolle sp. nov. and S. punctuscapum sp. nov., are described. A key to all known Sinodorcadion species (five) is presented.
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http://dx.doi.org/10.11646/zootaxa.3709.6.7DOI Listing
September 2015

A novel cold-active lipase from Candida albicans: cloning, expression and characterization of the recombinant enzyme.

Int J Mol Sci 2011 14;12(6):3950-65. Epub 2011 Jun 14.

School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China; E-Mails: (D.-M.L.); (N.Y.); (W.-K.W.); (Y.-F.S.).

A novel lipase gene lip5 from the yeast Candida albicans was cloned and sequenced. Alignment of amino acid sequences revealed that 86-34% identity exists with lipases from other Candida species. The lipase and its mutants were expressed in the yeast Pichia pastoris, where alternative codon usage caused the mistranslation of 154-Ser and 293-Ser as leucine. 154-Ser to leucine resulted in loss of expression of Lip5, and 293-Ser to leucine caused a marked reduction in the lipase activity. Lip5-DM, which has double mutations that revert 154 and 293 to serine residues, showed good lipase activity, and was overexpressed and purified by (NH(4))(2)SO(4) precipitation and ion-exchange chromatography. The pure Lip5-DM was stable at low temperatures ranging from 15-35 °C and pH 5-9, with the optimal conditions being 15-25 °C and pH 5-6. The activation energy of recombinant lipase was 8.5 Kcal/mol between 5 and 25 °C, suggesting that Lip5-DM was a cold-active lipase. Its activity was found to increase in the presence of Zn(2+), but it was strongly inhibited by Fe(2+), Fe(3+), Hg(2+) and some surfactants. In addition, the Lip5-DM could not tolerate water-miscible organic solvents. Lip5-DM exhibited a preference for the short-and medium-chain length p-nitrophenyl (C4 and C8 acyl group) esters rather than the long chain length p-nitrophenyl esters (C12, C16 and C18 acyl group) with highest activity observed with the C8 derivatives. The recombinant enzyme displayed activity toward triacylglycerols, such as olive oil and safflower oil.
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http://dx.doi.org/10.3390/ijms12063950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131601PMC
January 2015

[Review on the methods of processing rhubarb].

Zhong Xi Yi Jie He Xue Bao 2008 Nov;6(11):1184-9

Department of Processing Herbs of Traditional Chinese Medicine, College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province 330006, China.

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http://dx.doi.org/10.3736/jcim20091115DOI Listing
November 2008

Photonic crystal directional couplers formed by InAlGaAs nano-rods.

Opt Express 2005 Jan;13(1):38-43

This study demonstrates the use of photonic crystal directional couplers to separate light of wavelengths 1.31 and 1.55microm. The photonic crystal structure consists of InAlGaAs nano-rods arranged in square lattice. The coupling length of the light in the directional coupler at a wavelength of 1.31microm was designed to be four times greater than that at 1.55microm. This behavior helps in designing devices to split the two wavelengths. The devices are fabricated by e-beam lithography and conventional photolithography. The measurement results confirm that 1.31microm/1.55microm directional couplers can be realized in PC structures formed by nano-rods.
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http://dx.doi.org/10.1364/opex.13.000038DOI Listing
January 2005
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