Publications by authors named "Wen-Jie Fan"

11 Publications

  • Page 1 of 1

All-Trans Retinoic Acid Potentiates Antitumor Efficacy of Cisplatin by Increasing Differentiation of Cancer Stem-Like Cells in Cervical Cancer.

Ann Clin Lab Sci 2021 Jan;51(1):22-29

Department of Radiology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Objective: All-trans retinoic acid (ATRA), a derivative of vitamin A, has been reported to exert its synergistic antitumor effect with chemotherapy in various cancer types; however, its effect in cervical cancer remains unclear. The objective of this study was to investigate the antitumor effect of ATRA with or without cisplatin and elucidate its potential mechanisms in cervical cancer cells.

Methods: Cell viability was determined by CCK-8 assay. Cell cycle and cell apoptosis were analyzed by flow cytometry. Caspase-3, cleaved caspase-3 and cell cycle-related proteins were detected by western blotting. Scratch wound-healing assay was performed to evaluate cell migration ability. The expression of CD44, a biomarker of cervical cancer stem-like cells, was determined by flow cytometry and western blotting. In addition, the activity of the Wnt signaling pathway was monitored by luciferase reporter assay and western blotting.

Results: Compared with cisplatin treatment alone, combined use of ATRA and cisplatin significantly inhibited cell proliferation (<0.01) and migration (<0.01), and induced G0/G1 phase arrest (<0.01) and apoptosis (<0.05) in cervical cancer Hela cells. Furthermore, ATRA treatment also effectively induced differentiation of cancer stem-like cells as represented by reduced expression of CD44 and inhibited Wnt signaling pathway activity.

Conclusions: ATRA significantly enhanced the antitumor effect of cisplatin in cervical cancer cells, the mechanism of which might be attributed to its effect of inducing the differentiation of cancer stem-like cells.
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January 2021

Design of novel conjugated microporous polymers for efficient adsorptive desulfurization of small aromatic sulfur molecules.

J Mol Graph Model 2020 12 3;101:107734. Epub 2020 Sep 3.

Experimental Centre of Chemistry, Faculty of Chemical, Environmental and Biological, Dalian University of Technology, Dalian, 116024, People's Republic of China. Electronic address:

This paper presents a computational study of the adsorptive desulfurization of small aromatic sulfur compounds by conjugated microporous polymers (CMPs). The density-functional tight-binding method augmented with an R dispersion correction is employed to investigate the physisorption binding mechanism and electronic properties of the CMP-aromatic sulfur complexes. We show that the widely extended π conjugation in the CMP skeletons is favorable for the non-covalent adsorption of aromatic thiophene and dibenzothiophene via π-π, H-π, and S-π interactions. The average binding energies are calculated to be -6.2 ∼ -15.2 kcal/mol for CMP- thiophene/dibenzothiophene systems. For the dibenzothiophene molecule with larger size and more extended conjugation, it binds more than twice stronger to CMP than the thiophene molecule. We show that the replacement of quinoline unit to the phenylene group in the network linker effectively enhances the average binding capacities by around 0.8-1.8 kcal/mol. Our calculations theoretically demonstrate that CMPs materials are kind of promising candidates for the adsorptive desulfurization of small aromatic sulfur compounds. This paper provides useful theoretical guidance for design of novel carbon-based adsorbents for adsorptive desulfurization.
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http://dx.doi.org/10.1016/j.jmgm.2020.107734DOI Listing
December 2020

Inhibition of Long Noncoding RNA CRNDE Increases Chemosensitivity of Medulloblastoma Cells by Targeting miR-29c-3p.

Oncol Res 2020 Feb 21;28(1):95-102. Epub 2019 Nov 21.

Department of Pediatric Neurosurgery, Qingdao Women and Children's HospitalQingdao, ShandongP.R. China.

Long noncoding RNA CRNDE (CRNDE) recently emerged as a carcinogenic promoter in various cancers including medulloblastoma. However, the functions and molecular mechanisms of CRNDE to the acquired drug resistance of medulloblastoma are still unclear. The transcript levels of CRNDE were examined in four medulloblastoma cell lines exposed to cisplatin treatment, and IC values were calculated. Effects of CRNDE knockdown or miR-29c-3p overexpression on cell viability, colony formation, apoptosis, migration, and invasion were assessed using the CCK-8, colony formation assay, flow cytometry, and Transwell assays, respectively. RNA pulldown and RNA-binding protein immunoprecipitation (RIP) were performed to confirm the molecular interactions between CRNDE and miR-29c-3p involved in medulloblastoma cells. The in vivo role of CRNDE knockdown or miR-29c-3p overexpression on tumor growth and apoptosis was evaluated in a xenograft mouse model of human medulloblastoma. The transcript levels of lncRNA CRNDE were significantly higher in cisplatin-treated tumor cells with higher IC values. Depletion of CRNDE inhibited tumor cell proliferation and colony formation, induced cell apoptosis, and suppressed migration and invasion in medulloblastoma cells. Moreover, overexpression of miR-29c-3p inhibited tumor cell proliferation and colony formation, migration, and invasion, and enhanced apoptosis and chemosensitivity to cisplatin. In addition, CRNDE was found to act as a miR-29c-3p sponge. Furthermore, in vivo experiments showed the CRNDE/miR-29c-3p interactions involved in medulloblastoma. Our study demonstrates that CRNDE acts as a critical mediator of proliferation, apoptosis, migration, invasion, and resistance to chemotherapeutics via binding to and negatively regulating miR-29c-3p in medulloblastoma cells. These results provide novel molecular targets for treatment of medulloblastoma.
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http://dx.doi.org/10.3727/096504019X15742472027401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851516PMC
February 2020

NF-κB is involved in the LPS-mediated proliferation and apoptosis of MAC-T epithelial cells as part of the subacute ruminal acidosis response in cows.

Biotechnol Lett 2016 Nov 22;38(11):1839-1849. Epub 2016 Jul 22.

Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, China.

Objectives: To determine the effect of NF-κB on cell proliferation and apoptosis, we investigate the expression of inflammation and apoptosis-related factors in the bovine mammary epithelial cell line, MAC-T.

Results: MAC-T cells were cultured in vitro and MTT and LDH assays used to determine the effects of lipopolysaccharide (LPS) on proliferation and cytotoxicity respectively. RT-PCR and western blotting were used to evaluate the effect of LPS and NF-κB inhibition [pyrrolidine dithiocarbamate (PDTC) treatment] on the expression of inflammation and apoptosis-related factors. LPS significantly inhibited MAC-T cell proliferation in a dose- and time-dependent manner. Furthermore, LPS promoted apoptosis while the NF-кB inhibitor PDTC attenuated this effect. After LPS treatment, the NF-кB signaling pathway was activated, and the expression of inflammation and apoptosis-related factors increased. When PDTC blocked NF-кB signaling, the expression of inflammation and apoptosis-related factors were decreased in MAC-T cells.

Conclusions: LPS activates the TLR4/NF-κB signaling pathway, inhibits proliferation and promotes apoptosis in MAC-T cells. NF-кB inhibition attenuates MAC-T cell apoptosis and TLR4/NF-κB signaling pathway. NF-кB inhibitor alleviating MAC-T cell apoptosis is presumably modulated by NF-кB.
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http://dx.doi.org/10.1007/s10529-016-2178-0DOI Listing
November 2016

Protective effects of dexmedetomidine on intestinal ischemia-reperfusion injury.

Exp Ther Med 2015 Aug 10;10(2):647-652. Epub 2015 Jun 10.

Department of Anesthesia, Qingdao Women and Children's Hospital, Qingdao, Shandong 266034, P.R. China.

Dexmedetomidine (DEX) has been hypothesized to possess anti-oxidative properties that may mitigate the damage caused by ischemia-reperfusion (IR) injury. The aim of the present study was to examine the effects of DEX on intestinal contractile activity, inflammation and apoptosis following intestinal IR injury. Intestinal IR injury was induced in rats by complete occlusion of the superior mesenteric artery for 60 min, followed by a 60-min reperfusion period. Rats received an intraperitoneal injection of 25 µg/kg DEX at 30 min prior to the mesenteric IR injury. Following reperfusion, segments of the terminal ileum were rapidly extracted and transferred into an isolated organ bath. The contractile responses to receptor-mediated acetylcholine (Ach) and non-receptor-mediated potassium chloride (KCl) were subsequently examined. Nitric oxide (NO) levels were determined and the expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, Bax and Bcl-2 were measured using an enzyme-linked immunosorbent assay. The levels of telomerase and caspase-3 were determined using reverse transcription-quantitative polymerase chain reaction. The results indicated that DEX treatment produced a significant reduction in the IR-induced contractile response to Ach and KCl in the intestinal tissue. Furthermore, DEX appeared to significantly ameliorate intestinal IR injury, in addition to reducing the production of NO. Similar reductions were observed in the intestinal expression levels of TNF-α and IL-6. In addition, DEX treatment resulted in a reduction in the expression levels of Bax in the intestinal tissues, while increasing those of Bcl-2, in addition to significantly increasing the mRNA levels of telomerase and caspase-3. Therefore, the present study indicated that NO, TNF-α and IL-6 may partially contribute to the pathogenesis of intestinal IR injury in addition to the increased expression levels of Bax, Bcl-2, telomerase and caspase-3. These findings suggest that DEX possesses beneficial anti-apoptotic and anti-inflammatory effects in intestinal tissue following bowel injury.
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http://dx.doi.org/10.3892/etm.2015.2561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509460PMC
August 2015

Homocysteine promotes intestinal fibrosis in rats with trinitrobenzene sulfonic acid-induced colitis.

Dig Dis Sci 2015 Feb 8;60(2):375-81. Epub 2014 Oct 8.

Department of Gastroenterology, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China,

Background And Aim: Previous studies have revealed significantly increased levels of plasma and mucosal homocysteine (Hcy) in patients with Crohn's disease (CD); however, whether Hcy is involved in intestinal fibrosis of CD remains unclear. This study aimed to investigate the effects of Hcy on intestinal fibrosis in TNBS/ethanol-induced colitis and to elucidate its potential mechanisms.

Methods: Sprague-Dawley rats were divided into 4 groups: normal control, normal + Hcy injection, TNBS model and TNBS model + Hcy injection. Hyperhomocysteinemia was induced by subcutaneous injection of Hcy. DAI, CMDI and HI were calculated to evaluate the severity of colitis. Masson trichrome staining was performed to assess the severity of fibrosis. The plasma and mucosal levels of Hcy were measured by HPLC-FD. The levels of IL-1β, IL-6, TNF-α, TGF-β1, CTGF, MMP-2,9 and collagen I, III in the colon were determined by ELISA, and the mRNA expressions of TGF-β1, MMP-2,9 and TIMP-1 were detected by RT-PCR.

Results: Hcy was found to increase the scores of DAI, CMDI and HI; levels of IL-1β, Il-6, TNF-α, TGF-β1, CTGF, MMP-2,9 and collagen I, III; and mRNA expressions of TGF-β1, MMP-2,9 and TIMP-1 in colonic tissue of rats with TNBS/ethanol-induced colitis.

Conclusions: Hcy promotes intestinal fibrosis in rats with TNBS/ethanol-induced colitis, the underlying mechanisms of which may be attributed to its effects of increasing inflammatory damage, promoting the expression of profibrogenic cytokines and influencing MMPs/TIMPs balance.
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http://dx.doi.org/10.1007/s10620-014-3379-9DOI Listing
February 2015

Electronic and vibrational properties of stable isomers of (SiO)n((0,±)) (n = 2-7) clusters.

J Phys Chem A 2014 Oct 17;118(39):8893-900. Epub 2014 Jun 17.

Department of Physics and Materials Science, City University of Hong Kong , Kowloon, Hong Kong SAR, China.

First-principles calculations based on density functional theory have been performed to explore the stable configurations, electronic structures, and vibrational spectra of neutral and charged silicon monoxide clusters (SiO)n((0,±)) (n = 2-7), which could be used as precursors in the synthesis of silicon nanowires. Our theoretical calculations provide new results on characteristic electron affinity, ionization potential, and vibrational spectroscopy, guiding future experiments in the synthesis of high-quality silicon nanowires. Specifically, as the number of SiO units n increases, IR spectra of (SiO)n(±) and Raman spectra of (SiO)n(-) show an evident blue shift, and Raman spectra of (SiO)n demonstrate a red shift. Moreover, most of the neutral silicon monoxide clusters have strong IR intensities and weak Raman activities, while most of the anionic counterparts have relatively weak IR intensities and strong Raman activities. Some other energetically competitive isomers of some (SiO)n((0,±)) species were also studied for comparison.
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http://dx.doi.org/10.1021/jp412682fDOI Listing
October 2014

Possible cage-like nanostructures formed by amino acids.

Org Biomol Chem 2012 Jul 23;10(26):5049-54. Epub 2012 May 23.

Department of Physics and Materials Sciences, City University of Hong Kong, Hong Kong SAR, China.

We report possible cage-like nanostructures formed by a representative amino acid, serine octamers and decamers, determined by binding energy calculations and molecular dynamic simulations using the density functional tight-binding method. We used the l-handed serine to construct complex conformers linked by hydrogen bonds. We found the structures linked by -COOH···O=C- to be the most stable conformers and the calculation of the vibrational modes of complexes further illustrated this result. We attempted to apply our cage-like structures to the delivery of C(20) and cycloserine as model molecules. Our results may shed light on the design of cage-like biocompatible complexes for drug delivery.
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http://dx.doi.org/10.1039/c2ob25558hDOI Listing
July 2012

[Flower species identification and coverage estimation based on hyperspectral remote sensing data in Hulunbeier grassland].

Guang Pu Xue Yu Guang Pu Fen Xi 2011 Oct;31(10):2778-83

RS and GIS Institute of Peking University, Beijing 100871, China.

Monitoring grassland species and area real-timely and accurately is of great significance in species diversity research, as well as in sustainable development of ecosystem. Flowers have their own unique spectral characteristics. Compared with the nutrient stage, species are more easily identified by florescence. So, florescence is a critical period for identification. In the present paper, spectral differences among such flowers as Galium verum Linn., Hemerocallis citrina Baroni, Serratula centauroides Linn., Clematis hexapetala Pall., Lilium concolor var. pulchellum, Lilium pumilum and Artemisia frigida Willd. Sp. Pl. were found, along with identification methods, by analyzing canopies spectra and parametrizing characteristics. Verification results showed that when the coverage of flowers was greater than 10%, the accuracy of identification methods would be higher than 90%. On this basis, linear unmixing model was adopted to calculate the area of flowers in quadrates. Results showed that linear unmixing model was an effective method for estimating the coverage of flowers in grassland because the accuracy was about 4%.
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October 2011

[Review of monitoring soil water content using hyperspectral remote sensing].

Guang Pu Xue Yu Guang Pu Fen Xi 2010 Nov;30(11):3067-71

Institute of Remote Sensing and Geographical Information System, Peking University, Beijing, China.

Soil water content is a key parameter in monitoring drought. In recent years, a lot of work has been done on monitoring soil water content based on hyperspectral remotely sensed data both at home and abroad. In the present review, theories, advantages and disadvantages of the monitoring methods using different bands are introduced first. Then the unique advantages, as well as the problems, of the monitoring method with the aid of hyperspectral remote sensing are analyzed. In addition, the impact of soil water content on soil reflectance spectrum and the difference between values at different wavelengths are summarized. This review lists and summarizes the quantitative relationships between soil water content and soil reflectance obtained through analyzing the physical mechanism as well as through statistical way. The key points, advantages and disadvantages of each model are also analyzed and evaluated. Then, the problems in experimental study are pointed out, and the corresponding solutions are proposed. At the same time, the feasibility of removing vegetation effect is discussed, when monitoring soil water content using hyperspectral remote sensing. Finally, the future research trend is prospected.
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November 2010

[Hyperspectral remote sensing monitoring of grassland degradation].

Guang Pu Xue Yu Guang Pu Fen Xi 2010 Oct;30(10):2734-8

Institute of Remote Sensing and Geographical Information System, Peking University, Beijing 100081, China.

The distributing of China's grassland is abroad and the status of grassland degradation is in serious condition. So achieving real-time and exactly grassland ecological monitoring is significant for the carbon cycle, as well as for climate and on regional economies. With the field measured spectra data as data source, hyperspectral remote sensing monitoring of grassland degradation was researched in the present article. The warm meadow grassland in Hulunbeier was chosen as a study object. Reflectance spectra of leaves and pure canopies of some dominant grassland species such as Leymus chinensis, Stipa krylovii and Artemisia frigid, as well as reflectance spectra of mixed grass community were measured. Using effective spectral feature parametrization methods, the spectral feature of leaves and pure canopies were extracted, so the constructive species and degenerate indicator species can be exactly distinguished. Verification results showed that the accuracy of spectral identification was higher than 95%. Taking it as the foundation, the spectra of mixed grass community were unmixed using linear mixing models, and the proportion of all the components was calculated, and the errors were less than 5%. The research results of this article provided the evidence of hyperspectral remote sensing monitoring of grassland degradation.
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October 2010